ATE547532T1 - Verfahren zur erzeugung von hochgradig diversen bibliotheken - Google Patents

Verfahren zur erzeugung von hochgradig diversen bibliotheken

Info

Publication number
ATE547532T1
ATE547532T1 AT99932081T AT99932081T ATE547532T1 AT E547532 T1 ATE547532 T1 AT E547532T1 AT 99932081 T AT99932081 T AT 99932081T AT 99932081 T AT99932081 T AT 99932081T AT E547532 T1 ATE547532 T1 AT E547532T1
Authority
AT
Austria
Prior art keywords
nucleic acid
library
fragments
templates
strand
Prior art date
Application number
AT99932081T
Other languages
English (en)
Inventor
Richard Wagner
Martin Wright
Brent Kreider
Original Assignee
Bristol Myers Squibb Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bristol Myers Squibb Co filed Critical Bristol Myers Squibb Co
Application granted granted Critical
Publication of ATE547532T1 publication Critical patent/ATE547532T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT99932081T 1998-06-29 1999-06-29 Verfahren zur erzeugung von hochgradig diversen bibliotheken ATE547532T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9097098P 1998-06-29 1998-06-29
PCT/US1999/014776 WO2000000632A1 (en) 1998-06-29 1999-06-29 Methods for generating highly diverse libraries

Publications (1)

Publication Number Publication Date
ATE547532T1 true ATE547532T1 (de) 2012-03-15

Family

ID=22225171

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99932081T ATE547532T1 (de) 1998-06-29 1999-06-29 Verfahren zur erzeugung von hochgradig diversen bibliotheken

Country Status (13)

Country Link
EP (1) EP1092039B1 (de)
JP (1) JP4700805B2 (de)
KR (1) KR20010071613A (de)
CN (1) CN1308683A (de)
AT (1) ATE547532T1 (de)
AU (1) AU761570B2 (de)
CA (1) CA2331926C (de)
ES (1) ES2381824T3 (de)
IL (1) IL140100A0 (de)
NO (1) NO20006675L (de)
NZ (1) NZ508287A (de)
WO (1) WO2000000632A1 (de)
ZA (1) ZA200007261B (de)

Families Citing this family (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US6902918B1 (en) 1998-05-21 2005-06-07 California Institute Of Technology Oxygenase enzymes and screening method
DE69922978T2 (de) 1998-10-06 2005-12-08 Emalfarb, Mark Aaron, Jupiter Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen
US7488590B2 (en) 1998-10-23 2009-02-10 Amgen Inc. Modified peptides as therapeutic agents
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US8457903B1 (en) 1999-01-19 2013-06-04 Codexis Mayflower Holdings, Llc Method and/or apparatus for determining codons
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
US7873477B1 (en) 2001-08-21 2011-01-18 Codexis Mayflower Holdings, Llc Method and system using systematically varied data libraries
WO2001032712A2 (en) 1999-11-03 2001-05-10 Maxygen, Inc. Antibody diversity generation
US7115403B1 (en) 2000-05-16 2006-10-03 The California Institute Of Technology Directed evolution of galactose oxidase enzymes
US6539102B1 (en) 2000-09-01 2003-03-25 Large Scale Proteomics Reference database
US6980674B2 (en) 2000-09-01 2005-12-27 Large Scale Proteomics Corp. Reference database
EP1341911A1 (de) * 2000-12-06 2003-09-10 Novozymes A/S Verfahren zur erzeugung einer polynukleotidbibliothek
US6783941B2 (en) 2000-12-06 2004-08-31 Novozymes A/S Method for producing a polynucleotide library in vitro by mismatch repair of heteroduplexes
US6689568B2 (en) 2001-02-01 2004-02-10 Agilent Technologies, Inc. Capture arrays using polypeptide capture agents
WO2002079468A2 (en) * 2001-02-02 2002-10-10 Large Scale Biology Corporation A method of increasing complementarity in a heteroduplex polynucleotide
AU2002307340A1 (en) 2001-04-16 2002-10-28 California Institute Of Technology Peroxide-driven cytochrome p450 oxygenase variants
US20030032028A1 (en) * 2001-06-12 2003-02-13 Gayle Dace In vitro capture of nucleic acids via modified oligonucleotides and magnetic beads
US7226768B2 (en) 2001-07-20 2007-06-05 The California Institute Of Technology Cytochrome P450 oxygenases
WO2003062417A1 (fr) * 2002-01-22 2003-07-31 Mitsubishi Chemical Corporation Produit de ligation arn-adn, et utilisation correspondante
EP2278509B1 (de) 2002-03-01 2014-11-19 Codexis Mayflower Holdings, LLC Verfahren, Systeme und Software zur Erkennung von funktionalen Biomolekülen
US7620500B2 (en) 2002-03-09 2009-11-17 Maxygen, Inc. Optimization of crossover points for directed evolution
WO2003089620A2 (en) 2002-04-19 2003-10-30 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
CA2494798A1 (en) 2002-08-06 2005-01-13 Verdia, Inc. Ap1 amine oxidase variants
EP2194133B1 (de) 2003-03-06 2015-12-02 BASF Enzymes LLC Amylasen, Nukleinsäuren, die diese codieren, und Verfahren zu deren Herstellung und Verwendung
CA2889013C (en) 2003-03-07 2018-07-17 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
HUE030493T2 (en) 2003-04-04 2017-05-29 Basf Enzymes Llc Pectate lyases, nucleic acids and processes encoding them for their production and use
SG155063A1 (en) 2003-04-29 2009-09-30 Pioneer Hi Bred Int Novel glyphosate-n-acetyltransferase (gat) genes
WO2005017105A2 (en) 2003-06-17 2005-02-24 California University Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome p450
WO2005003319A2 (en) 2003-07-02 2005-01-13 Diversa Corporation Glucanases, nucleic acids encoding them and methods for making and using them
DK1660646T3 (en) 2003-08-11 2015-03-09 California Inst Of Techn Thermostable peroxide-driven cytochrome P450 oxygenase variants and methods of use
WO2005021714A2 (en) 2003-08-11 2005-03-10 Diversa Corporation Laccases, nucleic acids encoding them and methods for making and using them
JP2005065575A (ja) * 2003-08-22 2005-03-17 Japan Science & Technology Agency フレームシャッフリングによるタンパク質分子多様性集団の作製
WO2006009676A2 (en) 2004-06-16 2006-01-26 Diversa Corporation Compositions and methods for enzymatic decolorization of chlorophyll
WO2006099207A2 (en) 2005-03-10 2006-09-21 Diversa Corporation Lyase enzymes, nucleic acids encoding them and methods for making and using them
AU2006227965B2 (en) 2005-03-15 2013-01-31 Bp Corporation North America Inc. Cellulases, nucleic acids encoding them and methods for making and using them
US11214817B2 (en) 2005-03-28 2022-01-04 California Institute Of Technology Alkane oxidation by modified hydroxylases
US8715988B2 (en) 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
EP1987142A4 (de) 2006-02-02 2009-07-15 Verenium Corp Esterasen und damit zusammenhängende nukleinsäuren und verfahren
EP2450439B1 (de) 2006-02-10 2013-11-27 Verenium Corporation Cellulose abbauende Enzyme, Nukleinsäuren, die diese codieren, und Verfahren zu deren Herstellung und Verwendung
CA2946924A1 (en) 2006-02-14 2007-08-23 Bp Corporation North America Inc. Xylanases, nucleic acids encoding them and methods for making and using them
EP2112227B1 (de) 2006-03-07 2013-05-15 Cargill, Incorporated Aldolasen, Nukleinsäuren zu ihrer Kodierung sowie Verfahren zu ihrer Herstellung und Verwendung
AU2007223086B2 (en) 2006-03-07 2013-09-26 Basf Enzymes Llc Aldolases, nucleic acids encoding them and methods for making and using them
MX369001B (es) 2006-08-04 2019-10-24 Basf Enzymes Llc Glucanasas, acidos nucleicos que las codifican, y metodos para hacerlas y usarlas.
EP2069513A4 (de) 2006-08-04 2012-03-21 California Inst Of Techn Verfahren und systeme zur selektiven fluorinierung organischer moleküle
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
EA015591B1 (ru) 2006-09-21 2011-10-31 Верениум Корпорейшн Фосфолипазы, кодирующие их нуклеиновые кислоты и способы их получения и применения
US8877478B2 (en) 2006-09-21 2014-11-04 Verenium Corporation Phytases, nucleic acids encoding them and methods for making and using them
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
DK2069490T4 (en) 2006-12-21 2018-04-23 Syngenta Participations Ag Amylases and Glucoamylases, Nucleic Acids Encoding Them, and Methods of Preparation and Use thereof
BRPI0807132A2 (pt) 2007-01-30 2018-12-04 Syngenta Participations Ag enzimas para o tratamento de lignocelulósicos, ácidos nucléicos que codificam as mesmas, e metodos e uso das mesmas
US8551751B2 (en) 2007-09-07 2013-10-08 Dyadic International, Inc. BX11 enzymes having xylosidase activity
AU2008307371B2 (en) 2007-10-03 2015-05-28 Bp Corporation North America Inc. Xylanases, nucleic acids encoding them and methods for making and using them
PL2238242T3 (pl) 2008-01-03 2015-06-30 Basf Enzymes Llc Transferazy i oksydoreduktazy, kwasy nukleinowe kodujące je oraz sposoby ich wytwarzania i zastosowania
DK2238261T3 (da) 2008-01-03 2014-02-10 Verenium Corp Isomeraser, nukleinsyrer der koder for dem og fremgangsmåder til fremstilling og anvendelse deraf
AU2009248931A1 (en) 2008-05-23 2009-11-26 E. I. Du Pont De Nemours And Company Novel DGAT genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
CN102227501B (zh) 2008-09-26 2016-11-16 托卡根公司 基因治疗载体和胞嘧啶脱氨酶
DK2432797T3 (en) 2009-05-21 2017-01-16 Basf Enzymes Llc PHYTASES, NUCLEIC ACIDS, CODING THESE, AND METHODS OF PRODUCING AND USING THEREOF
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
BR112013003224A2 (pt) 2010-08-13 2016-06-07 Pioneer Hi Bred Int "construto de promotor quimérico, cassete de expressão, vetor de expressão, método de obtenção de uma planta, método para regular a expressão de um polinucleotídeo de interesse, polinucleotídeo, método para expressar um polinucleotídeo de interesse"
US9322007B2 (en) 2011-07-22 2016-04-26 The California Institute Of Technology Stable fungal Cel6 enzyme variants
RU2014148769A (ru) 2012-05-04 2016-06-27 Е.И. Дюпон Де Немур Энд Компани Композиции и способы, включающие последовательности, характеризующиеся мегануклеазной активностью
JP6004423B2 (ja) * 2012-05-07 2016-10-05 国立研究開発法人産業技術総合研究所 遺伝子連結法およびそれを用いた単鎖抗体作製方法
BR112015023286A2 (pt) 2013-03-14 2018-03-06 Arzeda Corp polipeptídeo recombinante com atividade da dicamba descarboxilase, construto de polinucleotídeo, célula, método de produção de uma célula hospedeira compreendendo um polinucleotídeo heterólogo que codifica um polipeptídeo tendo atividade da dicamba descarboxilase, método para descarboxilar dicamba, um derivado de dicamba ou um metabolito de dicamba, método para a detecção de um polipeptideo e método para a detecção da presença de um polinucleotideo que codifica um polipeptideo tendo atividade da dicamba descarboxilase
EP2970995A1 (de) 2013-03-14 2016-01-20 Pioneer Hi-Bred International, Inc. Zusammensetzungen mit dicamba-decarboxylaseaktivität und verfahren zur verwendung
WO2014150914A2 (en) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Phi-4 polypeptides and methods for their use
CN106232820A (zh) 2013-08-16 2016-12-14 先锋国际良种公司 杀昆虫蛋白及其使用方法
UA120598C2 (uk) 2013-09-13 2020-01-10 Піонір Хай-Бред Інтернешнл, Інк. Днк-конструкція та спосіб її застосування
US10544191B2 (en) 2013-12-23 2020-01-28 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
CN106536545A (zh) 2014-02-07 2017-03-22 先锋国际良种公司 杀昆虫蛋白及其使用方法
WO2015120270A1 (en) 2014-02-07 2015-08-13 Pioneer Hi Bred International, Inc. Insecticidal proteins and methods for their use
UA126192C2 (uk) 2014-10-16 2022-08-31 Піонір Хай-Бред Інтернешнл, Інк. Інсектицидний білок та спосіб його застосування
EP3262175A4 (de) * 2015-02-25 2018-10-31 Jumpcode Genomics, Inc. Verfahren und zusammensetzungen zur in-silico-sequenzierung langer leseabschnitte
BR112017019419B1 (pt) 2015-03-11 2022-10-11 E. I. Du Pont De Nemours And Company Construto de dna, método de obtenção de uma planta transgênica e método para controlar uma população de praga de verme da raiz do milho
CN116333064A (zh) 2015-05-19 2023-06-27 先锋国际良种公司 杀昆虫蛋白及其使用方法
UA124495C2 (uk) 2015-08-06 2021-09-29 Піонір Хай-Бред Інтернешнл, Інк. Інсектицидний білок рослинного походження та спосіб його застосування
CN108575091A (zh) 2015-12-18 2018-09-25 先锋国际良种公司 杀昆虫蛋白及其使用方法
US11339427B2 (en) 2016-02-12 2022-05-24 Jumpcode Genomics, Inc. Method for target specific RNA transcription of DNA sequences
WO2017192560A1 (en) 2016-05-04 2017-11-09 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN109788735A (zh) 2016-07-01 2019-05-21 先锋国际良种公司 来自植物的杀昆虫蛋白及其使用方法
WO2018084936A1 (en) 2016-11-01 2018-05-11 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN110088123B (zh) 2016-12-14 2023-10-20 先锋国际良种公司 杀昆虫蛋白及其使用方法
WO2018118811A1 (en) 2016-12-22 2018-06-28 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA3052794A1 (en) 2017-02-08 2018-08-16 Pioneer Hi-Bred International, Inc. Insecticidal combinations of plant derived insecticidal proteins and methods for their use
EP3622076A1 (de) 2017-05-11 2020-03-18 Pioneer Hi-Bred International, Inc. Insektizide proteine und verfahren zu deren verwendung
CN110158157B (zh) * 2018-02-13 2021-02-02 浙江大学 基于模板材料合成长度固定和特定末端序列dna文库的方法
CN113874392A (zh) 2019-03-28 2021-12-31 丹尼斯科美国公司 工程化抗体
BR112022027035A2 (pt) 2020-07-14 2023-04-11 Pioneer Hi Bred Int Proteínas inseticidas e métodos para uso das mesmas
WO2023173084A1 (en) 2022-03-11 2023-09-14 University Of Rochester Cyclopeptibodies and uses thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994379A (en) * 1983-03-09 1991-02-19 Cetus Corporation Modified signal peptides
US5447839A (en) * 1988-09-09 1995-09-05 Hoffmann-La Roche Inc. Detection of human papillomavirus by the polymerase chain reaction
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5580730A (en) * 1994-08-19 1996-12-03 Olympus America, Inc. Enzyme digestion method for the detection of amplified DNA
DE69627189T2 (de) * 1995-12-15 2004-02-05 Duke University Verfahren zum nachweis und zur entfernung von mutantsequenzen, die während enzymatischer amplifikation entstehen
DK0973940T3 (da) * 1997-03-18 2008-10-20 Novozymes As En in vitro-fremgangsmåde til konstruktion af et DNA-bibliotek
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers

Also Published As

Publication number Publication date
IL140100A0 (en) 2002-02-10
CA2331926A1 (en) 2000-01-06
CN1308683A (zh) 2001-08-15
WO2000000632A1 (en) 2000-01-06
CA2331926C (en) 2013-09-10
JP2002519038A (ja) 2002-07-02
EP1092039A1 (de) 2001-04-18
AU4847099A (en) 2000-01-17
EP1092039B1 (de) 2012-02-29
JP4700805B2 (ja) 2011-06-15
ZA200007261B (en) 2002-05-15
KR20010071613A (ko) 2001-07-28
ES2381824T3 (es) 2012-05-31
AU761570B2 (en) 2003-06-05
NO20006675D0 (no) 2000-12-28
WO2000000632A9 (en) 2000-10-26
NZ508287A (en) 2003-08-29
NO20006675L (no) 2000-12-28
EP1092039A4 (de) 2003-07-02

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