ATE547517T1 - Verfahren zum screening einer lipase mit verbesserter enzymatischer aktivität unter verwendung eines hefe- oberflächenpräsentationsvektors und der lipase - Google Patents

Verfahren zum screening einer lipase mit verbesserter enzymatischer aktivität unter verwendung eines hefe- oberflächenpräsentationsvektors und der lipase

Info

Publication number
ATE547517T1
ATE547517T1 AT03795460T AT03795460T ATE547517T1 AT E547517 T1 ATE547517 T1 AT E547517T1 AT 03795460 T AT03795460 T AT 03795460T AT 03795460 T AT03795460 T AT 03795460T AT E547517 T1 ATE547517 T1 AT E547517T1
Authority
AT
Austria
Prior art keywords
lipase
enzymatic activity
screening
mutant
yeast surface
Prior art date
Application number
AT03795460T
Other languages
English (en)
Inventor
Eui-Sung Choi
Jung-Hoon Sohn
So-Young Kim
Original Assignee
Korea Res Inst Of Bioscience
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Res Inst Of Bioscience filed Critical Korea Res Inst Of Bioscience
Application granted granted Critical
Publication of ATE547517T1 publication Critical patent/ATE547517T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT03795460T 2002-09-13 2003-09-04 Verfahren zum screening einer lipase mit verbesserter enzymatischer aktivität unter verwendung eines hefe- oberflächenpräsentationsvektors und der lipase ATE547517T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2002-0055575A KR100475133B1 (ko) 2002-09-13 2002-09-13 효모 표면 발현 벡터를 이용하여 변이 리파제를스크리닝하는 방법 및 신규 변이 리파제
PCT/KR2003/001820 WO2004024954A1 (en) 2002-09-13 2003-09-04 Method for screening of a lipase having improved enzymatic activity using yeast surface display vector and the lipase

Publications (1)

Publication Number Publication Date
ATE547517T1 true ATE547517T1 (de) 2012-03-15

Family

ID=31987425

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03795460T ATE547517T1 (de) 2002-09-13 2003-09-04 Verfahren zum screening einer lipase mit verbesserter enzymatischer aktivität unter verwendung eines hefe- oberflächenpräsentationsvektors und der lipase

Country Status (8)

Country Link
US (1) US7736643B2 (de)
EP (1) EP1546405B1 (de)
JP (1) JP4927332B2 (de)
KR (1) KR100475133B1 (de)
CN (1) CN100595283C (de)
AT (1) ATE547517T1 (de)
AU (1) AU2003261629A1 (de)
WO (1) WO2004024954A1 (de)

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DE102006016023A1 (de) * 2006-04-05 2007-10-11 Basf Ag Funktionale Expression von Triacylglycerol-Lipasen
JP5438259B2 (ja) * 2006-04-13 2014-03-12 Ｂｉｏ−ｅｎｅｒｇｙ株式会社 カンジダ・アンタークティカ由来リパーゼｂを細胞表層に提示する酵母
US9187737B2 (en) 2006-06-27 2015-11-17 Basf Se Mutants of a pseudomonas glumae butynol esterase
CN101541956B (zh) * 2006-11-28 2012-04-18 诺维信公司 脂解酶变体
DK2245146T3 (en) 2007-12-20 2015-06-29 Basf Se NEW CALB muteins AND THEIR USE
KR101005821B1 (ko) * 2007-12-28 2011-01-05 (주)바이오버드 배트록소빈의 변이 뉴클레오타이드 서열, α－인자 분비 시그널 서열 변이체 및 이를 이용한 재조합 단백질의 제조방법및 이를 이용한 배트록소빈의 제조방법
KR101087760B1 (ko) 2009-01-05 2011-11-30 한국생명공학연구원 신규한 재조합 효모 균주 및 이를 이용한 에탄올 및 목적단백질의 동시 생산 방법
JP2010183885A (ja) * 2009-02-13 2010-08-26 Kobe Univ タンパク質の製造方法およびそれに用いる発現ベクター
CN101565713B (zh) * 2009-06-01 2011-07-06 华南理工大学 南极假丝酵母脂肪酶b基因及其在酵母展示中的应用
KR101137026B1 (ko) * 2009-10-30 2012-04-19 한국생명공학연구원 효모 변이주 및 이를 이용한 스쿠알렌의 생산 방법
CN102604911B (zh) * 2009-11-11 2013-05-15 江南大学 高活力华根霉脂肪酶突变体
CN101899427B (zh) * 2009-11-11 2012-05-23 江南大学 通过定向进化构建的活力提高的脂肪酶突变体
CN102604912B (zh) * 2009-11-11 2013-03-13 江南大学 高活力华根霉脂肪酶突变体
CN102242097A (zh) * 2010-05-14 2011-11-16 浙江大学 一种提高beta葡聚糖酶活力持续性的方法
CN102242106A (zh) * 2010-05-14 2011-11-16 浙江大学 一种提高谷氨酸脱羧酶活力持续性的方法
CN101974499B (zh) * 2010-08-13 2012-07-11 江南大学 热稳定性提高的脂肪酶突变体
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
CN102212602B (zh) * 2011-04-28 2013-08-14 浙江大学 酵母展示脂肪酶催化合成维生素a乳酸酯的方法
CN102212578B (zh) * 2011-04-28 2014-01-29 浙江大学 酵母展示脂肪酶催化合成果糖棕榈酸酯的方法
CN102212601B (zh) * 2011-04-28 2013-08-14 浙江大学 酵母展示脂肪酶催化合成维生素a棕榈酸酯的方法
KR101460228B1 (ko) 2011-11-18 2014-11-10 한국생명공학연구원 활성이 증진된 변이 리파제 및 이를 이용한 바이오디젤의 생산방법
KR101692966B1 (ko) * 2013-06-21 2017-01-04 한국생명공학연구원 세포벽 결함 효모 변이주를 이용한 재조합 단백질 분비능이 향상된 효모 균주의 스크리닝 방법
US20170218382A1 (en) * 2014-07-31 2017-08-03 National University Corporation Kobe University Secretion Signal Peptide, And Protein Secretory Production And Cell Surface Display Using Said Secretion Signal Peptide
CN104195151B (zh) * 2014-07-31 2016-08-24 江南大学 一种基于全基因组结合生物信息学特异性筛选华根霉脂肪酶基因的方法
CN105062909A (zh) * 2015-09-18 2015-11-18 南京林业大学 双脂肪酶细胞表面共展示工程菌及其构建方法和应用
CN108374018B (zh) * 2018-02-26 2021-07-09 沈阳农业大学 提高α-凝集素锚定酿酒酵母表面展示α-半乳糖苷酶表达活性及稳定性的方法
CN112368375A (zh) * 2018-04-26 2021-02-12 巴斯夫欧洲公司 脂肪酶
KR102181315B1 (ko) * 2018-11-01 2020-11-20 한국생명공학연구원 활성 개선된 리조무코르 미에헤이 유래 변이 리파제 및 효모를 이용한 재조합 생산 방법
CN109576244B (zh) * 2018-12-06 2021-05-25 天津科技大学 一种新型脂肪酶及其制备与应用
CN111996132A (zh) * 2020-07-21 2020-11-27 郑州大学 一种解脂耶氏酵母表面展示方法
CN114058526A (zh) * 2021-11-18 2022-02-18 广西科学院 一种细胞表面展示碱性脂肪酶的酿酒酵母工程菌及其制备方法和使用方法
CN117070496B (zh) * 2023-10-16 2023-12-19 深圳昇瑞生物科技有限公司 一种南极假丝酵母脂肪酶b突变体及其制备方法和应用

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JP2002533087A (ja) * 1998-12-21 2002-10-08 ジェネンカー インターナショナル，インコーポレイティド 複数荷電変種を有する化学修飾酵素
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Also Published As

Publication number Publication date
KR100475133B1 (ko) 2005-03-10
EP1546405A1 (de) 2005-06-29
JP2005538711A (ja) 2005-12-22
WO2004024954A1 (en) 2004-03-25
JP4927332B2 (ja) 2012-05-09
US7736643B2 (en) 2010-06-15
AU2003261629A1 (en) 2004-04-30
EP1546405B1 (de) 2012-02-29
CN100595283C (zh) 2010-03-24
US20060275760A1 (en) 2006-12-07
CN1681942A (zh) 2005-10-12
EP1546405A4 (de) 2006-01-18
KR20040024074A (ko) 2004-03-20

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