ATE483810T1 - Entkopplung von dna-propagierung und proteinexpression für phagen-display - Google Patents

Entkopplung von dna-propagierung und proteinexpression für phagen-display

Info

Publication number
ATE483810T1
ATE483810T1 AT03784882T AT03784882T ATE483810T1 AT E483810 T1 ATE483810 T1 AT E483810T1 AT 03784882 T AT03784882 T AT 03784882T AT 03784882 T AT03784882 T AT 03784882T AT E483810 T1 ATE483810 T1 AT E483810T1
Authority
AT
Austria
Prior art keywords
uncoupling
phage
protein expression
propagation
phage display
Prior art date
Application number
AT03784882T
Other languages
English (en)
Inventor
Pietro Ciceri
Patrick Zarrinkar
Daniel Treiber
David Lockhart
Original Assignee
Ambit Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ambit Biosciences Corp filed Critical Ambit Biosciences Corp
Application granted granted Critical
Publication of ATE483810T1 publication Critical patent/ATE483810T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
AT03784882T 2002-08-07 2003-08-01 Entkopplung von dna-propagierung und proteinexpression für phagen-display ATE483810T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/214,654 US7112435B1 (en) 2002-08-07 2002-08-07 Uncoupling of DNA insert propagation and expression of protein for phage display
PCT/US2003/024221 WO2004015142A1 (en) 2002-08-07 2003-08-01 Uncoupling of dna propagation and expression of protein for phage display

Publications (1)

Publication Number Publication Date
ATE483810T1 true ATE483810T1 (de) 2010-10-15

Family

ID=31714248

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03784882T ATE483810T1 (de) 2002-08-07 2003-08-01 Entkopplung von dna-propagierung und proteinexpression für phagen-display

Country Status (10)

Country Link
US (1) US7112435B1 (de)
EP (1) EP1543160B1 (de)
JP (2) JP4870351B2 (de)
AT (1) ATE483810T1 (de)
AU (1) AU2003257132B2 (de)
CA (1) CA2493716A1 (de)
DE (1) DE60334466D1 (de)
DK (1) DK1543160T3 (de)
ES (1) ES2350575T3 (de)
WO (1) WO2004015142A1 (de)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7897381B2 (en) * 2002-08-07 2011-03-01 Ambit Biosciences Corporation Uncoupling of DNA insert propagation and expression of protein for phage display
US7833741B2 (en) * 2002-08-07 2010-11-16 Ambit Biosciences Corporation Uncoupling of DNA insert propagation and expression of protein for phage display
US20060068421A1 (en) * 2004-08-05 2006-03-30 Biosite, Inc. Compositions and methods for phage display of polypeptides
GB2428293A (en) * 2005-07-13 2007-01-24 Domantis Ltd Phage display libraries
ES2456275T3 (es) 2009-02-27 2014-04-21 Ambit Biosciences Corporation Derivados de quinazolina moduladores de la quinasa JAK y su uso en métodos
JP2013502429A (ja) 2009-08-19 2013-01-24 アムビト ビオスシエンセス コルポラチオン ビアリール化合物及びその使用方法
WO2011056764A1 (en) 2009-11-05 2011-05-12 Ambit Biosciences Corp. Isotopically enriched or fluorinated imidazo[2,1-b][1,3]benzothiazoles
WO2011150201A2 (en) 2010-05-27 2011-12-01 Ambit Biosciences Corporation Azolyl amide compounds and methods of use thereof
US9296722B2 (en) 2010-05-27 2016-03-29 Ambit Biosciences Corporation Azolyl urea compounds and methods of use thereof
US20130317045A1 (en) 2010-09-01 2013-11-28 Ambit Biosciences Corporation Thienopyridine and thienopyrimidine compounds and methods of use thereof
WO2012030924A1 (en) 2010-09-01 2012-03-08 Ambit Biosciences Corporation Azolopyridine and azolopyrimidine compounds and methods of use thereof
US20130225578A1 (en) 2010-09-01 2013-08-29 Ambit Biosciences Corporation 7-cyclylquinazoline derivatives and methods of use thereof
EP2611793A1 (de) 2010-09-01 2013-07-10 Ambit Biosciences Corporation 2-cyclochinazolinderivate und verwendungsverfahren dafür
US20130296363A1 (en) 2010-09-01 2013-11-07 Ambit Biosciences Corporation Quinoline and isoquinoline derivatives for use as jak modulators
CN103298805A (zh) 2010-09-01 2013-09-11 埃姆比特生物科学公司 喹唑啉化合物及其使用方法
JP5933554B2 (ja) 2010-09-01 2016-06-15 アムビト ビオスシエンセス コルポラチオン 光学活性のあるピラゾリルアミノキナゾリン及びその医薬組成物及び使用方法
EP2611794A1 (de) 2010-09-01 2013-07-10 Ambit Biosciences Corporation 4--azolylaminochinazolinderivate und verwendungsverfahren dafür
KR102066297B1 (ko) 2011-10-14 2020-01-14 암비트 바이오사이언시즈 코포레이션 헤테로사이클릭 화합물 및 이의 유형 3 수용체 티로신 키나아제의 조절인자로서의 용도
WO2017019804A2 (en) 2015-07-28 2017-02-02 Plexxikon Inc. Compounds and methods for kinase modulation, and indications therefor
WO2024020328A1 (en) 2022-07-16 2024-01-25 Eurofins Discoverx Corporation Binding assays using phage display technology and kits thereof

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Publication number Priority date Publication date Assignee Title
US5693489A (en) 1984-03-30 1997-12-02 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5498538A (en) 1990-02-15 1996-03-12 The University Of North Carolina At Chapel Hill Totally synthetic affinity reagents
US6172197B1 (en) 1991-07-10 2001-01-09 Medical Research Council Methods for producing members of specific binding pairs
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US5770356A (en) * 1992-09-04 1998-06-23 The Scripps Research Institute Phagemids coexpressing a surface receptor and a surface heterologous protein
CA2150262C (en) 1992-12-04 2008-07-08 Kaspar-Philipp Holliger Multivalent and multispecific binding proteins, their manufacture and use
US5766905A (en) 1996-06-14 1998-06-16 Associated Universities Inc. Cytoplasmic bacteriophage display system
US20030124555A1 (en) * 2001-05-21 2003-07-03 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
US6541011B2 (en) 1998-02-11 2003-04-01 Maxygen, Inc. Antigen library immunization
JP2002522029A (ja) * 1998-07-27 2002-07-23 ジェネンテック・インコーポレーテッド コートタンパク質の改変によるファージ提示における改良した形質転換効率
CA2383186A1 (en) 1999-09-03 2001-03-15 Yale University Method to identify gene function using small molecule probes
US7060506B2 (en) 2000-01-31 2006-06-13 Cyclacel, Ltd. Compositions and methods for monitoring the modification of modification dependent binding partner polypeptides
EP2796546B1 (de) 2001-04-19 2017-08-09 The Scripps Research Institute Aufnahme von unnatürlichen Aminosäuren
US20030186221A1 (en) 2002-04-02 2003-10-02 Lockhart David J. Phage display affinity filter and forward screen

Also Published As

Publication number Publication date
EP1543160B1 (de) 2010-10-06
AU2003257132A1 (en) 2004-02-25
US7112435B1 (en) 2006-09-26
JP4870351B2 (ja) 2012-02-08
DE60334466D1 (de) 2010-11-18
JP5389882B2 (ja) 2014-01-15
EP1543160A4 (de) 2007-03-21
WO2004015142A1 (en) 2004-02-19
AU2003257132B2 (en) 2011-12-15
DK1543160T3 (da) 2011-01-17
JP2005534333A (ja) 2005-11-17
CA2493716A1 (en) 2004-02-19
JP2012050444A (ja) 2012-03-15
ES2350575T3 (es) 2011-01-25
EP1543160A1 (de) 2005-06-22

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