EA200702009A1 - Усовершенствованный способ экспрессирующего клонирования в клетке-хозяине - Google Patents
Усовершенствованный способ экспрессирующего клонирования в клетке-хозяинеInfo
- Publication number
- EA200702009A1 EA200702009A1 EA200702009A EA200702009A EA200702009A1 EA 200702009 A1 EA200702009 A1 EA 200702009A1 EA 200702009 A EA200702009 A EA 200702009A EA 200702009 A EA200702009 A EA 200702009A EA 200702009 A1 EA200702009 A1 EA 200702009A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- host cell
- improved method
- cloning
- expressing
- interest
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Изобретение относится к промоторной последовательности ДНК, предназначенной для использования в усовершенствованном способе экспрессирующего клонирования для выделения ДНК-последовательностей, содержащих последовательность ДНК, кодирующую интересующий протеин в клетке-хозяине, и для усовершенствованного способа экспрессирующего клонирования, в котором применение осуществляется этим промотором. Выделенные последовательности ДНК являются пригодными в способах для продуцирования протеина, вызывающего интерес.
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP05102160 | 2005-03-18 | ||
EP05102159 | 2005-03-18 | ||
EP05102180 | 2005-03-18 | ||
EP05102184 | 2005-03-18 | ||
EP05102177 | 2005-03-18 | ||
PCT/EP2006/060864 WO2006097539A2 (en) | 2005-03-18 | 2006-03-20 | Improved method of expression cloning in a host cell |
Publications (1)
Publication Number | Publication Date |
---|---|
EA200702009A1 true EA200702009A1 (ru) | 2008-02-28 |
Family
ID=36942192
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA200702009A EA200702009A1 (ru) | 2005-03-18 | 2006-03-20 | Усовершенствованный способ экспрессирующего клонирования в клетке-хозяине |
Country Status (7)
Country | Link |
---|---|
US (1) | US20090209007A1 (ru) |
EP (1) | EP1859036A2 (ru) |
JP (1) | JP2008532535A (ru) |
AU (1) | AU2006224538A1 (ru) |
CA (1) | CA2599640A1 (ru) |
EA (1) | EA200702009A1 (ru) |
WO (1) | WO2006097539A2 (ru) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102007031947A1 (de) | 2007-07-06 | 2009-01-08 | Helmholtz-Zentrum Für Umweltforschung Gmbh - Ufz | Allergene aus Aspergillus versicolor und Verfahren zum Nachweis einer innenraumbedingten Schimmelpilzallergie |
IL214246B (en) * | 2011-07-21 | 2018-08-30 | Shafferman Avigdor | Vaccines produced from htra-defective Bacillus anthraxis |
ES2758096T3 (es) * | 2015-03-25 | 2020-05-04 | Senseup Gmbh | Sensores para la detección y cuantificación de secreción microbiológica de proteínas |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69838106T2 (de) * | 1997-12-22 | 2008-04-03 | Dsm Ip Assets B.V. | Expressionsklonierung in filamentösen pilzen |
-
2006
- 2006-03-20 US US11/886,585 patent/US20090209007A1/en not_active Abandoned
- 2006-03-20 AU AU2006224538A patent/AU2006224538A1/en not_active Abandoned
- 2006-03-20 EA EA200702009A patent/EA200702009A1/ru unknown
- 2006-03-20 JP JP2008501336A patent/JP2008532535A/ja not_active Withdrawn
- 2006-03-20 WO PCT/EP2006/060864 patent/WO2006097539A2/en active Application Filing
- 2006-03-20 CA CA002599640A patent/CA2599640A1/en not_active Abandoned
- 2006-03-20 EP EP06708799A patent/EP1859036A2/en not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
WO2006097539A3 (en) | 2006-11-30 |
EP1859036A2 (en) | 2007-11-28 |
CA2599640A1 (en) | 2006-09-21 |
AU2006224538A1 (en) | 2006-09-21 |
WO2006097539A2 (en) | 2006-09-21 |
JP2008532535A (ja) | 2008-08-21 |
US20090209007A1 (en) | 2009-08-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EA200901110A1 (ru) | Рекомбинантная клетка-хозяин для получения соединения, представляющего интерес | |
EA201070764A1 (ru) | Полипептиды, обладающие активностью, усиливающей лизис целлюлозы, и кодирующие их полинуклеотиды | |
DK1836299T4 (da) | Polypeptider, der har cellobiohydrolaseaktivitet, og polynukleotider, der koder for disse | |
DK1831362T3 (da) | Syreaktive svampeproteaser | |
MX2009012846A (es) | Polipeptidos que tienen actividad celulosica mejorada y polinucleotidos que los codifican. | |
EA201490216A1 (ru) | Варианты альфа-амилазы и кодирующие их полинуклеотиды | |
EP3805348A3 (en) | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same | |
EA201071323A1 (ru) | Axmi-115, axmi-113, axmi-005, axmi-163, axmi-184 инсектицидные белки и способы их применения | |
ATE512980T1 (de) | Polypeptide mit beta-glucosidase-aktivität sowie diese codierende polynukleotide | |
MX2014001383A (es) | Colageno 7 y metodos relacionados. | |
NO20075088L (no) | Vertscelle omfattende en vektor for fremstilling av proteiner som krever gamma-karboksylering | |
MX356647B (es) | Polipeptidos que tienen actividad de fosfolipasa c y polinucleotidos que codifican los mismos. | |
IL180531A0 (en) | Novel sequence for improving expression of nucleic acid | |
ATE533838T1 (de) | Verfahren zur verbesserten einführung von dna in bakterienzellen | |
WO2005047319A3 (en) | Dna sequence and expressed recombinant glycoproteins related to feline thyrotropin | |
DE502004011932D1 (de) | Verfahren zur herstellung von 3-methylamino-1-(thien-2-yl)-propan-1-ol | |
DK1877551T4 (da) | Polypeptider, der har endoglucanaseaktivitet, og polynukleotider, der koder for samme | |
ATE445009T1 (de) | Signalpeptid zur herstellung eines polypeptids | |
MX2010009071A (es) | Peptidos aislados que tienen actividad inhibitoria de fosfolipasa. | |
EA200702009A1 (ru) | Усовершенствованный способ экспрессирующего клонирования в клетке-хозяине | |
WO2010133668A3 (en) | Eukaryotic host cell comprising an expression enhancer | |
ATE427996T1 (de) | Verfahren zur herstellung von (s)-butan-2-ol | |
KR100717353B1 (ko) | 피키아 파스토리스 유래의 자동 유도성 nps 프로모터 및이를 이용한 이종 단백질의 제조방법 | |
WO2008002548A3 (en) | Novel human acidic mammalian chitinase and use thereof | |
MX2017016501A (es) | Aminopeptidasas para hidrolizados de proteinas. |