ATE483032T1 - Verfahren zur herstellung von oligonukleotiden - Google Patents

Verfahren zur herstellung von oligonukleotiden

Info

Publication number
ATE483032T1
ATE483032T1 AT06706145T AT06706145T ATE483032T1 AT E483032 T1 ATE483032 T1 AT E483032T1 AT 06706145 T AT06706145 T AT 06706145T AT 06706145 T AT06706145 T AT 06706145T AT E483032 T1 ATE483032 T1 AT E483032T1
Authority
AT
Austria
Prior art keywords
oligonucleotides
nicking
cassette
production
producing oligonucleotides
Prior art date
Application number
AT06706145T
Other languages
English (en)
Inventor
Jorn Koch
Magnus Stougaard
Jakob Schwalbe Lohmann
Original Assignee
Olink Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olink Ab filed Critical Olink Ab
Application granted granted Critical
Publication of ATE483032T1 publication Critical patent/ATE483032T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT06706145T 2005-04-12 2006-04-10 Verfahren zur herstellung von oligonukleotiden ATE483032T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200500521 2005-04-12
PCT/DK2006/050012 WO2006108423A2 (en) 2005-04-12 2006-04-10 Methods for production of oligonucleotides

Publications (1)

Publication Number Publication Date
ATE483032T1 true ATE483032T1 (de) 2010-10-15

Family

ID=36942313

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06706145T ATE483032T1 (de) 2005-04-12 2006-04-10 Verfahren zur herstellung von oligonukleotiden

Country Status (6)

Country Link
US (1) US8080393B2 (de)
EP (1) EP1871897B1 (de)
CN (1) CN101213310A (de)
AT (1) ATE483032T1 (de)
DE (1) DE602006017192D1 (de)
WO (1) WO2006108423A2 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008534015A (ja) * 2005-03-31 2008-08-28 ハミルトン ソーン バイオサイエンシーズ インコーポレイテッド 環状化可能な核酸プローブ及び増幅法
US8492098B2 (en) 2006-02-21 2013-07-23 The Trustees Of Tufts College Methods and arrays for target analyte detection and determination of reaction components that affect a reaction
GB0901593D0 (en) 2009-01-30 2009-03-11 Touchlight Genetics Ltd Production of closed linear DNA
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
GB201013153D0 (en) 2010-08-04 2010-09-22 Touchlight Genetics Ltd Primer for production of closed linear DNA
US9952237B2 (en) 2011-01-28 2018-04-24 Quanterix Corporation Systems, devices, and methods for ultra-sensitive detection of molecules or particles
US20120295801A1 (en) * 2011-02-17 2012-11-22 President And Fellows Of Harvard College High-Throughput In Situ Hybridization
JP6093498B2 (ja) * 2011-12-13 2017-03-08 株式会社日立ハイテクノロジーズ 核酸増幅方法
US9862994B2 (en) * 2012-02-09 2018-01-09 Dana-Farber Cancer Institute, Inc. Selective nucleic acid amplification from nucleic acid pools
US9193994B2 (en) 2012-04-20 2015-11-24 Samsung Electronics Co., Ltd. Polynucleotide and use thereof
KR101984700B1 (ko) * 2012-11-19 2019-05-31 삼성전자주식회사 폴리뉴클레오티드 및 그의 용도
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
KR102257912B1 (ko) 2013-03-13 2021-05-27 메소 스케일 테크놀러지즈, 엘엘시 개선된 분석 방법
CN103276074B (zh) * 2013-05-21 2015-08-19 昆山彭济凯丰生物科技有限公司 一种合成短单链脱氧核糖核苷酸探针的方法
US9828599B2 (en) 2013-09-18 2017-11-28 General Electric Company Methods of selecting binding-elements and uses thereof
US10359419B2 (en) 2013-10-02 2019-07-23 General Electric Company Methods for detection of target using affinity binding
GB201321123D0 (en) 2013-11-29 2014-01-15 Linea Ab Q Amplification of circular molecules
KR20230022268A (ko) 2014-05-15 2023-02-14 메소 스케일 테크놀러지즈, 엘엘시 개선된 분석 방법
WO2016130943A1 (en) 2015-02-13 2016-08-18 Rana Therapeutics, Inc. Hybrid oligonucleotides and uses thereof
GB201502645D0 (en) * 2015-02-17 2015-04-01 Touchlight Genetics Ltd Method
CN106554957B (zh) 2015-09-30 2020-04-21 中国农业科学院深圳农业基因组研究所 测序文库及其制备和应用
GB201621514D0 (en) 2016-12-16 2017-02-01 Q-Linea Ab Padlock probe detection method
GB201901583D0 (en) * 2019-02-05 2019-03-27 Ducani Cosimo Method and products for producing funtionaslised single stranded oligonucleotides
GB201905651D0 (en) * 2019-04-24 2019-06-05 Lightbio Ltd Nucleic acid constructs and methods for their manufacture
WO2022208326A1 (en) * 2021-03-30 2022-10-06 Pixelgen Technologies Ab Rolling circle amplification product having multiple free ends

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
WO1992001813A1 (en) 1990-07-25 1992-02-06 Syngene, Inc. Circular extension for generating multiple nucleic acid complements
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
AU8997991A (en) 1991-01-31 1992-08-06 Becton Dickinson & Company Exonuclease mediated strand displacement amplification
JP3080178B2 (ja) 1991-02-18 2000-08-21 東洋紡績株式会社 核酸配列の増幅方法およびそのための試薬キット
JP3085409B2 (ja) 1991-03-29 2000-09-11 東洋紡績株式会社 標的核酸配列の検出方法およびそのための試薬キット
GB9117910D0 (en) 1991-08-20 1991-10-09 Nat Res Dev Oligonucleotides and their use in an assay
JPH07500734A (ja) 1991-10-31 1995-01-26 ユニヴァーシティ オブ ピッツバーグ オリゴヌクレオチドプローブのポリマーを用いる核酸配列の検出方法
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US5733733A (en) 1992-08-04 1998-03-31 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US6180338B1 (en) 1992-08-04 2001-01-30 Beckman Coulter, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences
US5614389A (en) 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5714320A (en) 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US6077668A (en) 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
US6096880A (en) * 1993-04-15 2000-08-01 University Of Rochester Circular DNA vectors for synthesis of RNA and DNA
US7135312B2 (en) 1993-04-15 2006-11-14 University Of Rochester Circular DNA vectors for synthesis of RNA and DNA
WO1995035390A1 (en) 1994-06-22 1995-12-28 Mount Sinai School Of Medicine Of The City University Of New York Ligation-dependent amplification for the detection of infectious pathogens and abnormal genes
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5876924A (en) 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
USRE38442E1 (en) 1994-06-22 2004-02-24 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
JP3974941B2 (ja) 1995-11-21 2007-09-12 イェール ユニバーシティ 単分子セグメントの増幅および検出
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
ES2262902T3 (es) 1995-12-05 2006-12-01 Jorn Erland Koch Una reaccion en cascada de amplificacion de acido nucleico.
WO1999049079A1 (en) 1998-03-25 1999-09-30 Ulf Landegren Rolling circle replication of padlock probes
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
FR2794755A1 (fr) 1999-06-14 2000-12-15 Inst Nat Sante Rech Med Procede de circularisation d'oligonucleotides autour d'un acide nucleique en double brin, les structures obtenues et leurs applications
US6221603B1 (en) 2000-02-04 2001-04-24 Molecular Dynamics, Inc. Rolling circle amplification assay for nucleic acid analysis
AU2001293336A1 (en) 2000-04-11 2001-10-23 Ulf Landegren Nucleic acid detection medium
AU2002239679A1 (en) 2000-12-20 2002-07-01 The Regents Of The University Of California Rolling circle amplification detection of rna and dna
EP1314783B1 (de) * 2001-11-22 2008-11-19 Sloning BioTechnology GmbH Nukleinsäure-Linker und deren Verwendung in der Gensynthese
GB0230238D0 (en) 2002-12-28 2003-02-05 Fu Guoliang Combined exponential and linear amplification
US20040086892A1 (en) * 2002-11-06 2004-05-06 Crothers Donald M. Universal tag assay
EP1567669B1 (de) 2002-12-02 2010-03-24 Illumina Cambridge Limited Bestimmung der methylierung von nukleinsäuresequenzen
JP2007524395A (ja) 2003-06-25 2007-08-30 ソーマジェニックス インコーポレイテッド 標的依存性の環状化及びトポロジー的結合が可能なポリヌクレオチド
US20050069929A1 (en) * 2003-08-08 2005-03-31 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
US20050074804A1 (en) * 2003-09-26 2005-04-07 Youxiang Wang Amplification of polynucleotide sequences by rolling circle amplification

Also Published As

Publication number Publication date
EP1871897B1 (de) 2010-09-29
WO2006108423A3 (en) 2007-01-25
EP1871897A2 (de) 2008-01-02
DE602006017192D1 (de) 2010-11-11
CN101213310A (zh) 2008-07-02
US8080393B2 (en) 2011-12-20
US20100028953A1 (en) 2010-02-04
WO2006108423A2 (en) 2006-10-19

Similar Documents

Publication Publication Date Title
ATE483032T1 (de) Verfahren zur herstellung von oligonukleotiden
DE60323340D1 (de) Verfahren zur herstellung interferierender rna-moleküle in säugetierzellen und therapeutische anwendungen solcher moleküle
SG10201408300PA (en) Processing of amplified dna fragments for sequencing
AR039717A1 (es) Construcciones de arn de intrones cadena doble y usos de las mismas
ATE469983T1 (de) Rolling-circle rna-amplifikation
WO2005081714A3 (en) Use of interfering rna in the production of transgenic animals
ATE500337T1 (de) Verfahren zur durchführung homologer rekombinationen in pflanzen
ATE510930T1 (de) Zusammensetzungen und verfahren zur bearbeitung und amplifikation von dna mit verwendung mehrerer enzyme in einer einzigen reaktion
WO2009148560A8 (en) Methods and compositions for nucleic acid sequencing
MX2007004321A (es) Micro acidos ribonucleicos.
ATE474910T1 (de) L-glutaminsäure produzierender mikroorganismus sowie verfahren zur produktion von l- glutaminsäure
DE502006005991D1 (de) Verfahren zur herstellung von polyisocyanurat hartschaum
WO2007014075A3 (en) Rnai modulation of the rho-a gene in research models
ATE400650T1 (de) Verfahren zur herstellung von nukleinsäuremolekülen
WO2005110067A3 (en) Method and compositions for rna interference
DE602005017252D1 (de) Verfahren zur amplifikation von nukleinsäuren mittels promotor-templates
ATE446374T1 (de) Verfahren zur enantioselektiven enzymatischen reduktion von ketoverbindungen
ATE452881T1 (de) Katalysiertes verfahren zur herstellung von c-5- substituierten heterocyclischen inhibitoren von 11beta-hydroxysteroiddehydrogenase vom typ 1
ATE526312T1 (de) Verfahren zur herstellung von reinen 4- pyrrolidinophenylbenzyletherderivaten als maob- inhibitoren
ATE491688T1 (de) Verfahren zur herstellung von 2-substituierten 5- (1-alkylthio)alkylpyridinen
DE60123334D1 (de) Methode für die Produktion von Nukleotiden durch Fermentierung
DE602006013596D1 (de) Mutageneseverfahren
ATE491035T1 (de) Verfahren zur abtrennung selektionierbarer markergensequenzen
ATE529382T1 (de) Verbessertes verfahren zur herstellung von blausäure
WO2005074560A3 (en) Compositions and methods that enhance rna interference

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties