ATE469983T1 - Rolling-circle rna-amplifikation - Google Patents

Rolling-circle rna-amplifikation

Info

Publication number
ATE469983T1
ATE469983T1 AT03796961T AT03796961T ATE469983T1 AT E469983 T1 ATE469983 T1 AT E469983T1 AT 03796961 T AT03796961 T AT 03796961T AT 03796961 T AT03796961 T AT 03796961T AT E469983 T1 ATE469983 T1 AT E469983T1
Authority
AT
Austria
Prior art keywords
molecules
rna molecules
sequence
strand cdna
cdna
Prior art date
Application number
AT03796961T
Other languages
English (en)
Inventor
Gyanendra Kumar
Patricio Abarzua
Original Assignee
Qiagen Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh filed Critical Qiagen Gmbh
Application granted granted Critical
Publication of ATE469983T1 publication Critical patent/ATE469983T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT03796961T 2002-12-31 2003-12-11 Rolling-circle rna-amplifikation ATE469983T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/335,573 US6977153B2 (en) 2002-12-31 2002-12-31 Rolling circle amplification of RNA
PCT/US2003/039430 WO2004061119A2 (en) 2002-12-31 2003-12-11 Rolling circle amplification of rna

Publications (1)

Publication Number Publication Date
ATE469983T1 true ATE469983T1 (de) 2010-06-15

Family

ID=32655391

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03796961T ATE469983T1 (de) 2002-12-31 2003-12-11 Rolling-circle rna-amplifikation

Country Status (8)

Country Link
US (2) US6977153B2 (de)
EP (1) EP1585833B1 (de)
JP (1) JP2006512081A (de)
AT (1) ATE469983T1 (de)
AU (1) AU2003297891A1 (de)
CA (1) CA2512196A1 (de)
DE (1) DE60332873D1 (de)
WO (1) WO2004061119A2 (de)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US20040121338A1 (en) * 2002-12-19 2004-06-24 Alsmadi Osama A. Real-time detection of rolling circle amplification products
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US7955795B2 (en) 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
WO2004070053A2 (en) * 2003-02-03 2004-08-19 Amersham Biosciences Corporation cDNA AMPLIFICATION FOR EXPRESSION PROFILING
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US20040248103A1 (en) * 2003-06-04 2004-12-09 Feaver William John Proximity-mediated rolling circle amplification
WO2005010159A2 (en) * 2003-07-17 2005-02-03 Children's Hospital Medical Center Rolling circle amplification of micro-rna samples
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
JP2008506165A (ja) * 2004-06-18 2008-02-28 リール・トゥー・リミテッド データ集合の目録作成および探索のための方法およびシステム
WO2006003721A1 (en) * 2004-07-02 2006-01-12 Kabushiki Kaisha Dnaform Method for preparing sequence tags
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7302146B2 (en) * 2004-09-17 2007-11-27 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
WO2006076650A2 (en) * 2005-01-12 2006-07-20 Applera Corporation Compositions, methods, and kits for selective amplification of nucleic acids
EP1863908B1 (de) 2005-04-01 2010-11-17 Qiagen GmbH Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
EP2620510B2 (de) 2005-06-15 2020-02-19 Complete Genomics Inc. Einzelne Molekülarrays für genetische und chemische Analyse
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US7960104B2 (en) 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
US20070168197A1 (en) * 2006-01-18 2007-07-19 Nokia Corporation Audio coding
WO2007092538A2 (en) * 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
EP1994180A4 (de) 2006-02-24 2009-11-25 Callida Genomics Inc Genomsequenzierung auf dna-arrays mit hohem durchsatz
SG10201405158QA (en) 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
DE102006020885A1 (de) * 2006-05-05 2007-11-08 Qiagen Gmbh Einführung von Sequenzelementen in Nukleinsäuren
US20080035085A1 (en) * 2006-08-14 2008-02-14 Hendriksma Nick J Method and apparatus for controlling a switchable cam follower
US7910302B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US7897344B2 (en) 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US7901890B2 (en) 2007-11-05 2011-03-08 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
US8617811B2 (en) 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US8518640B2 (en) 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US8298768B2 (en) 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
FR2924440B1 (fr) * 2007-12-04 2015-01-09 Pf Medicament Nouveau procede de generation et de criblage d'une banque d'anticorps
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
CN104862383B (zh) 2008-03-28 2019-05-28 加利福尼亚太平洋生物科学股份有限公司 用于核酸测序的组合物和方法
EP2336358A1 (de) * 2008-05-06 2011-06-22 QIAGEN GmbH Gleichzeitiger Nachweis mehrerer Nukleinsäuresequenzen in einer Reaktion
US8962253B2 (en) 2009-04-13 2015-02-24 Somagenics Inc. Methods and compositions for detection of small RNAs
EP2248915B1 (de) * 2009-05-05 2013-09-18 Qiagen GmbH Detektion mehrerer Nukleinsäuresequenzen in einer Reaktionskartusche
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
CN110016499B (zh) 2011-04-15 2023-11-14 约翰·霍普金斯大学 安全测序***
KR102034721B1 (ko) * 2011-12-15 2019-10-22 삼성전자주식회사 시료 중 rna 종의 존재 비율을 결정하는 방법
US20130157259A1 (en) * 2011-12-15 2013-06-20 Samsung Electronics Co., Ltd. Method of amplifying dna from rna in sample and use thereof
US9422601B2 (en) 2011-12-15 2016-08-23 Samsung Electronics Co., Ltd. Method of determining a ratio of RNA species in a sample
EP2794926B1 (de) 2011-12-22 2018-01-17 SomaGenics Inc. Verfahren zur herstellung kleiner rna-bibliotheken und ihre verwendung zur expressionsprofilierung von ziel-rnas
WO2014004852A2 (en) * 2012-06-29 2014-01-03 General Electric Company Kit for isothermal dna amplification starting from an rna template
US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
KR101922124B1 (ko) * 2012-08-27 2018-11-26 삼성전자주식회사 시료 중 ｒｎａ로부터 ｄｎａ를 증폭하는 방법
CA2886605A1 (en) * 2012-09-28 2014-04-03 Cepheid Two-primer pcr for microrna multiplex assay
US11525163B2 (en) 2012-10-29 2022-12-13 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
JP6112652B2 (ja) * 2012-11-29 2017-04-12 国立大学法人広島大学 標的ｄｎａの特異的増幅方法
US20140302504A1 (en) 2013-03-15 2014-10-09 Theranos, Inc. Nucleic Acid Amplification
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
EP3587592A1 (de) * 2013-03-15 2020-01-01 Theranos IP Company, LLC Nukleinsäureamplifikation
AU2014233150A1 (en) 2013-03-15 2015-09-17 Theranos Ip Company, Llc Nucleic acid amplification
US10655167B2 (en) 2013-07-26 2020-05-19 General Electric Company Ligase-assisted nucleic acid circularization and amplification
US9217167B2 (en) * 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
JP2016537009A (ja) 2013-09-06 2016-12-01 セラノス， インコーポレイテッド 感染症の検出のためのシステム及び方法
US20160338421A1 (en) 2015-05-21 2016-11-24 Marla Hope Caprow-D'Avanzo Post-breast surgery bra
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
WO2017106683A2 (en) 2015-12-18 2017-06-22 Massachusetts Institute Of Technology Concatemeric rna molecules, compositions, and methods and uses thereof
US11014957B2 (en) 2015-12-21 2021-05-25 Realseq Biosciences, Inc. Methods of library construction for polynucleotide sequencing
WO2017192512A2 (en) 2016-05-02 2017-11-09 Massachusetts Institute Of Technology Amphiphilic nanoparticles for delivery of crispr based therapy
US10077459B2 (en) 2016-05-04 2018-09-18 General Electric Company Cell-free protein expression using rolling circle amplification product
DK3519612T3 (da) * 2016-10-01 2022-06-20 Berkeley Lights Inc Dna-stregkodesammensætninger og fremgansgmåder til in situ-identificering i en mikrofluidanordning
SG11202001010UA (en) 2017-08-07 2020-03-30 Univ Johns Hopkins Methods and materials for assessing and treating cancer
SG11202101934SA (en) 2018-07-30 2021-03-30 Readcoor Llc Methods and systems for sample processing or analysis
WO2021114135A1 (zh) * 2019-12-11 2021-06-17 清华大学 基于阻断型链式聚合扩增反应体外快速合成中高拷贝dna重复序列
CN111647576B (zh) * 2020-06-24 2021-02-09 南京诺唯赞生物科技股份有限公司 一种热稳定性逆转录酶突变体及其应用

Family Cites Families (101)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2264423A (en) * 1939-08-29 1941-12-02 Roy L Wingenroth Blast furnace receiving hopper
US3687808A (en) * 1969-08-14 1972-08-29 Univ Leland Stanford Junior Synthetic polynucleotides
US4469863A (en) * 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5023243A (en) * 1981-10-23 1991-06-11 Molecular Biosystems, Inc. Oligonucleotide therapeutic agent and method of making same
US4476301A (en) * 1982-04-29 1984-10-09 Centre National De La Recherche Scientifique Oligonucleotides, a process for preparing the same and their application as mediators of the action of interferon
US4994373A (en) * 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US5118800A (en) * 1983-12-20 1992-06-02 California Institute Of Technology Oligonucleotides possessing a primary amino group in the terminal nucleotide
US4748111A (en) * 1984-03-12 1988-05-31 Molecular Diagnostics, Inc. Nucleic acid-protein conjugate used in immunoassay
US5550111A (en) * 1984-07-11 1996-08-27 Temple University-Of The Commonwealth System Of Higher Education Dual action 2',5'-oligoadenylate antiviral derivatives and uses thereof
FR2567892B1 (fr) * 1984-07-19 1989-02-17 Centre Nat Rech Scient Nouveaux oligonucleotides, leur procede de preparation et leurs applications comme mediateurs dans le developpement des effets des interferons
US5367066A (en) * 1984-10-16 1994-11-22 Chiron Corporation Oligonucleotides with selectably cleavable and/or abasic sites
US5242794A (en) * 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
FR2575751B1 (fr) * 1985-01-08 1987-04-03 Pasteur Institut Nouveaux nucleosides de derives de l'adenosine, leur preparation et leurs applications biologiques
US5185444A (en) * 1985-03-15 1993-02-09 Anti-Gene Deveopment Group Uncharged morpolino-based polymers having phosphorous containing chiral intersubunit linkages
US5034506A (en) * 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5405938A (en) * 1989-12-20 1995-04-11 Anti-Gene Development Group Sequence-specific binding polymers for duplex nucleic acids
US5166315A (en) * 1989-12-20 1992-11-24 Anti-Gene Development Group Sequence-specific binding polymers for duplex nucleic acids
US5235033A (en) * 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5264423A (en) * 1987-03-25 1993-11-23 The United States Of America As Represented By The Department Of Health And Human Services Inhibitors for replication of retroviruses and for the expression of oncogene products
US5276019A (en) * 1987-03-25 1994-01-04 The United States Of America As Represented By The Department Of Health And Human Services Inhibitors for replication of retroviruses and for the expression of oncogene products
DE3851889T2 (de) * 1987-06-24 1995-04-13 Florey Howard Inst Nukleosid-derivate.
US4924624A (en) * 1987-10-22 1990-05-15 Temple University-Of The Commonwealth System Of Higher Education 2,',5'-phosphorothioate oligoadenylates and plant antiviral uses thereof
US5188897A (en) * 1987-10-22 1993-02-23 Temple University Of The Commonwealth System Of Higher Education Encapsulated 2',5'-phosphorothioate oligoadenylates
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
WO1989009221A1 (en) * 1988-03-25 1989-10-05 University Of Virginia Alumni Patents Foundation Oligonucleotide n-alkylphosphoramidates
US5278302A (en) * 1988-05-26 1994-01-11 University Patents, Inc. Polynucleotide phosphorodithioates
US5216141A (en) * 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5175273A (en) * 1988-07-01 1992-12-29 Genentech, Inc. Nucleic acid intercalating agents
US4984957A (en) * 1988-08-08 1991-01-15 Kubota, Ltd. Work-implement adapter for front loader
US5198543A (en) * 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5001050A (en) * 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5134066A (en) * 1989-08-29 1992-07-28 Monsanto Company Improved probes using nucleosides containing 3-dezauracil analogs
JP2581833B2 (ja) * 1989-09-11 1997-02-12 株式会社日立製作所 プラントの運転状態監視システム
US5591722A (en) 1989-09-15 1997-01-07 Southern Research Institute 2'-deoxy-4'-thioribonucleosides and their antiviral activity
US5399676A (en) * 1989-10-23 1995-03-21 Gilead Sciences Oligonucleotides with inverted polarity
US5264562A (en) * 1989-10-24 1993-11-23 Gilead Sciences, Inc. Oligonucleotide analogs with novel linkages
DK0942000T3 (da) * 1989-10-24 2004-11-01 Isis Pharmaceuticals Inc 2'-modificerede oligonukleotider
US5264564A (en) * 1989-10-24 1993-11-23 Gilead Sciences Oligonucleotide analogs with novel linkages
US5177198A (en) * 1989-11-30 1993-01-05 University Of N.C. At Chapel Hill Process for preparing oligoribonucleoside and oligodeoxyribonucleoside boranophosphates
US5130302A (en) * 1989-12-20 1992-07-14 Boron Bilogicals, Inc. Boronated nucleoside, nucleotide and oligonucleotide compounds, compositions and methods for using same
US5587361A (en) * 1991-10-15 1996-12-24 Isis Pharmaceuticals, Inc. Oligonucleotides having phosphorothioate linkages of high chiral purity
US5459255A (en) * 1990-01-11 1995-10-17 Isis Pharmaceuticals, Inc. N-2 substituted purines
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
US5321131A (en) * 1990-03-08 1994-06-14 Hybridon, Inc. Site-specific functionalization of oligodeoxynucleotides for non-radioactive labelling
US5470967A (en) * 1990-04-10 1995-11-28 The Dupont Merck Pharmaceutical Company Oligonucleotide analogs with sulfamate linkages
GB9009980D0 (en) * 1990-05-03 1990-06-27 Amersham Int Plc Phosphoramidite derivatives,their preparation and the use thereof in the incorporation of reporter groups on synthetic oligonucleotides
EP0745689A3 (de) * 1990-05-11 1996-12-11 Microprobe Corporation Teststab für einen Nukleinsäure-Hybridisierungstest
US5489677A (en) * 1990-07-27 1996-02-06 Isis Pharmaceuticals, Inc. Oligonucleoside linkages containing adjacent oxygen and nitrogen atoms
US5541307A (en) * 1990-07-27 1996-07-30 Isis Pharmaceuticals, Inc. Backbone modified oligonucleotide analogs and solid phase synthesis thereof
US5177196A (en) * 1990-08-16 1993-01-05 Microprobe Corporation Oligo (α-arabinofuranosyl nucleotides) and α-arabinofuranosyl precursors thereof
US5214134A (en) * 1990-09-12 1993-05-25 Sterling Winthrop Inc. Process of linking nucleosides with a siloxane bridge
US5561225A (en) * 1990-09-19 1996-10-01 Southern Research Institute Polynucleotide analogs containing sulfonate and sulfonamide internucleoside linkages
JPH06505704A (ja) 1990-09-20 1994-06-30 ギリアド　サイエンシズ，インコーポレイテッド 改変ヌクレオシド間結合
US5432272A (en) * 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5264567A (en) * 1990-10-22 1993-11-23 Mect Corporation GM3 analogous compound
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5489667A (en) 1991-03-20 1996-02-06 Atochem Deutschland Gmbh Polyetheresteramides and process for making and using the same
US5539082A (en) * 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5571799A (en) * 1991-08-12 1996-11-05 Basco, Ltd. (2'-5') oligoadenylate analogues useful as inhibitors of host-v5.-graft response
US5273638A (en) * 1991-09-30 1993-12-28 Beckman Instruments, Inc. Nucleotide sequence determination employing matched dideoxynucleotide terminator concentrations
ES2103918T3 (es) * 1991-10-17 1997-10-01 Ciba Geigy Ag Nucleosidos biciclicos, oligonucleotidos, procedimiento para su obtencion y productos intermedios.
US5594121A (en) 1991-11-07 1997-01-14 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified purines
US5412087A (en) * 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
US5484908A (en) * 1991-11-26 1996-01-16 Gilead Sciences, Inc. Oligonucleotides containing 5-propynyl pyrimidines
US5359044A (en) * 1991-12-13 1994-10-25 Isis Pharmaceuticals Cyclobutyl oligonucleotide surrogates
DE4216120A1 (de) * 1992-05-15 1993-11-18 Bayer Ag å-Phthalocyanine
US5434257A (en) * 1992-06-01 1995-07-18 Gilead Sciences, Inc. Binding compentent oligomers containing unsaturated 3',5' and 2',5' linkages
US5547843A (en) * 1992-07-17 1996-08-20 Associated Universities, Inc. Method for promoting specific alignment of short oligonucleotides on nucleic acids
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5476925A (en) * 1993-02-01 1995-12-19 Northwestern University Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups
GB9304618D0 (en) * 1993-03-06 1993-04-21 Ciba Geigy Ag Chemical compounds
ATE155467T1 (de) * 1993-03-30 1997-08-15 Sanofi Sa Acyclische nucleosid analoge und sie enthaltende oligonucleotidsequenzen
US6096880A (en) * 1993-04-15 2000-08-01 University Of Rochester Circular DNA vectors for synthesis of RNA and DNA
US5502177A (en) * 1993-09-17 1996-03-26 Gilead Sciences, Inc. Pyrimidine derivatives for labeled binding partners
JP3334279B2 (ja) * 1993-09-30 2002-10-15 カシオ計算機株式会社 ピッチ表示装置
US5429807A (en) * 1993-10-28 1995-07-04 Beckman Instruments, Inc. Method and apparatus for creating biopolymer arrays on a solid support surface
US5457187A (en) * 1993-12-08 1995-10-10 Board Of Regents University Of Nebraska Oligonucleotides containing 5-fluorouracil
US5446137B1 (en) * 1993-12-09 1998-10-06 Behringwerke Ag Oligonucleotides containing 4'-substituted nucleotides
US5519134A (en) * 1994-01-11 1996-05-21 Isis Pharmaceuticals, Inc. Pyrrolidine-containing monomers and oligomers
US5596091A (en) 1994-03-18 1997-01-21 The Regents Of The University Of California Antisense oligonucleotides comprising 5-aminoalkyl pyrimidine nucleotides
EP0684316B1 (de) * 1994-04-29 2004-10-20 Johnson & Johnson Clinical Diagnostics, Inc. Homogenes Verfahren zum Nachweis von Doppelstrang-Nukleinsäuren mittels fluoreszierender Farbstoffe und dafür nützliche Kits
US5525711A (en) * 1994-05-18 1996-06-11 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Pteridine nucleotide analogs as fluorescent DNA probes
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US5451203A (en) * 1994-07-29 1995-09-19 Orthopedic Systems, Inc. Traction mechanism
US5597909A (en) 1994-08-25 1997-01-28 Chiron Corporation Polynucleotide reagents containing modified deoxyribose moieties, and associated methods of synthesis and use
US5516134A (en) * 1994-09-20 1996-05-14 Perfect Season, Inc. Seat for a wheeled vehicle
US5451067A (en) * 1994-10-18 1995-09-19 Pieper; Julius A. Chuck key with integral holder
US5563912A (en) * 1995-02-27 1996-10-08 Nec Corporation High efficiency speech coding apparatus and transit switching system employing the same
US5648245A (en) * 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6203984B1 (en) * 1998-07-02 2001-03-20 Pacron, Inc. Proportional amplification of mRNA from a linear template in vitro
US6274320B1 (en) * 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
AU783841B2 (en) * 1999-11-26 2005-12-15 454 Life Sciences Corporation Nucleic acid probe arrays
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences

Also Published As

Publication number Publication date
JP2006512081A (ja) 2006-04-13
WO2004061119A3 (en) 2004-11-11
US20040126770A1 (en) 2004-07-01
EP1585833B1 (de) 2010-06-02
WO2004061119A2 (en) 2004-07-22
DE60332873D1 (de) 2010-07-15
US6977153B2 (en) 2005-12-20
EP1585833A2 (de) 2005-10-19
US20060188893A1 (en) 2006-08-24
CA2512196A1 (en) 2004-07-22
AU2003297891A1 (en) 2004-07-29
EP1585833A4 (de) 2007-02-14

Similar Documents

Publication Publication Date Title
ATE469983T1 (de) Rolling-circle rna-amplifikation
Van Dijk et al. Library preparation methods for next-generation sequencing: tone down the bias
BR0205268A (pt) Processos e composições para a mplificação de sequências de rna
DE60328214D1 (de) Verfahren und zusammensetzungen in verbindung mit gen-silencing
ATE483032T1 (de) Verfahren zur herstellung von oligonukleotiden
WO2003008538A3 (en) Gene expression profiling
ATE464381T1 (de) Rna-amplifikation auf ligationsbasis
ATE480644T1 (de) Verfahren zur quantifizierung kleiner rna- moleküle
ATE470726T1 (de) Polynukleotidamplifikation
WO2004092418A3 (en) Global amplification using a randomly primed composite primer
ATE461292T1 (de) Erstellung von expressionsprofilen unter verwendung von mikroarrays
CA2464691A1 (en) Methods and systems for dynamic gene expression profiling
DE60320780D1 (de) Funktionalisierte nanopartikel und verwendungsverfahren
CN106868005B (zh) 一种用于高效快速扩增cDNA末端的锚定引物及扩增方法
WO2006135684A3 (en) Methods and kits for sense rna synthesis
ATE488605T1 (de) Verfahren zur quantitativen bewertung einer umordnung oder einer gezielten genetischen rekombination bei einem individuum sowie verwendungen davon
AR030961A1 (es) Secuencias de nucleotidos que intervienen en la fertilidad masculina en plantas y metodo de uso de las mismas
ATE446384T1 (de) Verfahren zur amplifikation von nukleinsäuren mittels promotor-templates
CN102534813A (zh) 构建中小片段rna测序文库的方法
CN110157785A (zh) 一种单细胞rna测序文库构建方法
WO2006110161A3 (en) Method for identification and quantification of short or small rna molecules
CN108251503A (zh) 一种快速构建链特异性rna高通量测序文库的方法
WO2002083943A3 (de) Mikroarray-verfahren zur anreicherung von dna-fragmenten aus komplexen mischungen
CN105039501A (zh) 制备rna探针的方法及装置
CN109750092A (zh) 一种靶向富集高gc含量目标dna的方法和试剂盒

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties