ATE196510T1 - Verwendung eines osmolyten zur verringerung oder aufhebung von nichtkovalenten bindungen biologischer moleküle an inerten oberflächen - Google Patents

Verwendung eines osmolyten zur verringerung oder aufhebung von nichtkovalenten bindungen biologischer moleküle an inerten oberflächen

Info

Publication number
ATE196510T1
ATE196510T1 AT96108278T AT96108278T ATE196510T1 AT E196510 T1 ATE196510 T1 AT E196510T1 AT 96108278 T AT96108278 T AT 96108278T AT 96108278 T AT96108278 T AT 96108278T AT E196510 T1 ATE196510 T1 AT E196510T1
Authority
AT
Austria
Prior art keywords
osmolyte
biological molecules
reduce
covalent bonding
remove non
Prior art date
Application number
AT96108278T
Other languages
English (en)
Inventor
Elmar Maier
Igor Ivanov
Original Assignee
Qiagen Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh filed Critical Qiagen Gmbh
Application granted granted Critical
Publication of ATE196510T1 publication Critical patent/ATE196510T1/de

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)
AT96108278T 1996-05-23 1996-05-23 Verwendung eines osmolyten zur verringerung oder aufhebung von nichtkovalenten bindungen biologischer moleküle an inerten oberflächen ATE196510T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP96108278A EP0808906B1 (de) 1996-05-23 1996-05-23 Verwendung eines Osmolyten zur Verringerung oder Aufhebung von nichtkovalenten Bindungen biologischer Moleküle an inerten Oberflächen

Publications (1)

Publication Number Publication Date
ATE196510T1 true ATE196510T1 (de) 2000-10-15

Family

ID=8222814

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96108278T ATE196510T1 (de) 1996-05-23 1996-05-23 Verwendung eines osmolyten zur verringerung oder aufhebung von nichtkovalenten bindungen biologischer moleküle an inerten oberflächen

Country Status (4)

Country Link
EP (1) EP0808906B1 (de)
JP (1) JP4113603B2 (de)
AT (1) ATE196510T1 (de)
DE (1) DE69610410T2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1004676B1 (de) * 1997-08-14 2006-03-15 Takara Bio Inc. Methoden zur dns vervielfältigung und material dafür
ES2381279T3 (es) 2005-05-06 2012-05-24 Gen-Probe Incorporated Métodos y productos para la captura de ácido nucleico diana
JP7096482B2 (ja) * 2017-12-05 2022-07-06 藤倉化成株式会社 感作された不溶性担体粒子を含有する免疫測定試薬の劣化防止手段

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0153875A3 (de) * 1984-03-01 1987-06-24 The State Of Victoria Festphase-Enzymimmunanalyse und Testsatz
DE3717402A1 (de) * 1987-05-23 1988-12-08 Behringwerke Ag Verfahren zur bestimmung von proteinen und mittel dazu
US5047326A (en) * 1988-10-07 1991-09-10 Eastman Kodak Company Immunmological reagent composition and its use in the determination of chlamydial or gonococcal antigens
ES2106341T3 (es) * 1992-05-01 1997-11-01 Univ Pennsylvania Estructuras de deteccion microfabricadas.
DE69531487T2 (de) * 1994-01-31 2004-06-17 The Regents Of The University Of California, Oakland Methode zur eliminierung von sequenzierartefakten
US5512462A (en) * 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
DE4411588C1 (de) * 1994-03-30 1995-09-28 Deutsches Rheuma Forschungszen Puffer für DNA- und RNA-Polymerase-Reaktionen

Also Published As

Publication number Publication date
DE69610410D1 (de) 2000-10-26
JPH1066596A (ja) 1998-03-10
EP0808906A1 (de) 1997-11-26
EP0808906B1 (de) 2000-09-20
JP4113603B2 (ja) 2008-07-09
DE69610410T2 (de) 2001-05-10

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