AR241658A1 - Procedimiento para la preparacion de una proteina que consiste de aminoacidos geneticamente codificables. - Google Patents

Procedimiento para la preparacion de una proteina que consiste de aminoacidos geneticamente codificables.

Info

Publication number
AR241658A1
AR241658A1 AR88310742A AR31074288A AR241658A1 AR 241658 A1 AR241658 A1 AR 241658A1 AR 88310742 A AR88310742 A AR 88310742A AR 31074288 A AR31074288 A AR 31074288A AR 241658 A1 AR241658 A1 AR 241658A1
Authority
AR
Argentina
Prior art keywords
protein
gene
preparation
streptomyces
transforming
Prior art date
Application number
AR88310742A
Other languages
English (en)
Original Assignee
Hoechst Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Application granted granted Critical
Publication of AR241658A1 publication Critical patent/AR241658A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

PREPARACION DE UNA PROTEINA QUE CONSISTE DE AMINOACIDOS GENETICAMENTE CODIFICABLES, EN EL QUE SE CONSTRUYE UN GEN QUE CODIFICA UNA PROTEINA DE FUSION QUE CONSISTE DE UNA PROTEINA BACTERIANA Y LAPROTEINA DESEADA, INTRODUCIENDO ESTE GEN EN UN VECTOR, TRANSFORMANDO CON ESTE, UNA CELULA HUESPED BACTERIANA, EFECTUANDO EN LA CELULA HUESPED LA PROTEINA DE FUSION, AISLANDO Y DISOCIANDOLA LIBERANDO ASI LA PROTEINA DESEADA, EN DONDE LA PROTEINA BACTERIANA ES TENDAMISTATO EN FORMA NO MODIFICADA O MODIFICADA, LA CELULA HUESPED ES UNA CELULA DE STREPTOMYCES, LA PROTEINA DE FUSION ES SECRETADE LA STREPTOMYCES Y AISLADA DEL MEDIO DE CULTIVO. ESTA PROTEINA MANIFIESTA UN EFECTO INHIBIDOR DE LA ALFA-AMILASA.
AR88310742A 1987-05-05 1988-05-03 Procedimiento para la preparacion de una proteina que consiste de aminoacidos geneticamente codificables. AR241658A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19873714866 DE3714866A1 (de) 1987-05-05 1987-05-05 Verfahren zur herstellung von fremdproteinen in streptomyceten

Publications (1)

Publication Number Publication Date
AR241658A1 true AR241658A1 (es) 1992-10-30

Family

ID=6326841

Family Applications (1)

Application Number Title Priority Date Filing Date
AR88310742A AR241658A1 (es) 1987-05-05 1988-05-03 Procedimiento para la preparacion de una proteina que consiste de aminoacidos geneticamente codificables.

Country Status (19)

Country Link
EP (1) EP0289936B1 (es)
JP (1) JP2671260B2 (es)
KR (1) KR970001235B1 (es)
CN (1) CN1029989C (es)
AR (1) AR241658A1 (es)
AT (1) ATE94905T1 (es)
AU (1) AU612144B2 (es)
CA (1) CA1338338C (es)
DE (2) DE3714866A1 (es)
DK (1) DK175525B1 (es)
ES (1) ES2045004T3 (es)
FI (1) FI97549C (es)
HU (1) HU204094B (es)
IE (1) IE62522B1 (es)
IL (1) IL86277A0 (es)
NO (1) NO178036C (es)
NZ (1) NZ224464A (es)
PT (1) PT87400B (es)
ZA (1) ZA883168B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4012818A1 (de) * 1990-04-21 1991-10-24 Hoechst Ag Verfahren zur herstellung von fremdproteinen in streptomyceten
US5426036A (en) * 1987-05-05 1995-06-20 Hoechst Aktiengesellschaft Processes for the preparation of foreign proteins in streptomycetes
EP0367163B1 (de) * 1988-11-03 1996-01-03 Hoechst Aktiengesellschaft Verfahren zur Herstellung eines Insulin-Vorprodukts in Streptomyceten
DE3844211A1 (de) * 1988-12-29 1990-07-05 Hoechst Ag Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung
DE3936876A1 (de) * 1989-11-06 1991-05-23 Hoechst Ag Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3331860A1 (de) * 1983-09-03 1985-03-21 Hoechst Ag, 6230 Frankfurt Verfahren zur herstellung von tendamistat
DE3418274A1 (de) * 1984-05-17 1985-11-21 Hoechst Ag, 6230 Frankfurt Signalpeptid fuer die exkretion von peptiden in streptomyceten
EP0196056B1 (en) * 1985-03-28 1991-05-22 Chiron Corporation Improved expression using fused genes providing for protein product
DE3707150A1 (de) * 1987-03-06 1988-09-15 Hoechst Ag Tendamistat-derivate
EP0367163B1 (de) * 1988-11-03 1996-01-03 Hoechst Aktiengesellschaft Verfahren zur Herstellung eines Insulin-Vorprodukts in Streptomyceten

Also Published As

Publication number Publication date
NO881942L (no) 1988-11-07
DE3884261D1 (de) 1993-10-28
CN1029989C (zh) 1995-10-11
DK175525B1 (da) 2004-11-22
CN88102568A (zh) 1988-11-23
DE3714866A1 (de) 1988-11-24
EP0289936B1 (de) 1993-09-22
NO178036B (no) 1995-10-02
NO881942D0 (no) 1988-05-04
EP0289936A3 (en) 1989-11-23
FI97549C (fi) 1997-01-10
PT87400B (pt) 1992-09-30
IL86277A0 (en) 1988-11-15
KR970001235B1 (ko) 1997-02-04
NZ224464A (en) 1990-02-26
IE881338L (en) 1988-11-05
JPS63301793A (ja) 1988-12-08
FI882059A0 (fi) 1988-05-03
JP2671260B2 (ja) 1997-10-29
FI97549B (fi) 1996-09-30
HUT46941A (en) 1988-12-28
KR880014102A (ko) 1988-12-22
IE62522B1 (en) 1995-02-08
ATE94905T1 (de) 1993-10-15
FI882059A (fi) 1988-11-06
DK241688A (da) 1988-11-06
ZA883168B (en) 1989-11-29
DK241688D0 (da) 1988-05-04
ES2045004T3 (es) 1994-01-16
HU204094B (en) 1991-11-28
PT87400A (pt) 1989-05-31
CA1338338C (en) 1996-05-21
EP0289936A2 (de) 1988-11-09
NO178036C (no) 1996-01-10
AU612144B2 (en) 1991-07-04
AU1555988A (en) 1988-11-10

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