AR124445A1 - Proteínas inhibidoras de insectos novedosas - Google Patents
Proteínas inhibidoras de insectos novedosasInfo
- Publication number
- AR124445A1 AR124445A1 ARP210103592A ARP210103592A AR124445A1 AR 124445 A1 AR124445 A1 AR 124445A1 AR P210103592 A ARP210103592 A AR P210103592A AR P210103592 A ARP210103592 A AR P210103592A AR 124445 A1 AR124445 A1 AR 124445A1
- Authority
- AR
- Argentina
- Prior art keywords
- seq
- tic4029
- nucleic acid
- recombinant nucleic
- acid sequence
- Prior art date
Links
- 241000238631 Hexapoda Species 0.000 title abstract 2
- 108091006086 inhibitor proteins Proteins 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 abstract 11
- 108090000623 proteins and genes Proteins 0.000 abstract 11
- 230000000361 pesticidal effect Effects 0.000 abstract 9
- 150000007523 nucleic acids Chemical group 0.000 abstract 7
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 6
- 241000607479 Yersinia pestis Species 0.000 abstract 5
- 238000000034 method Methods 0.000 abstract 4
- 108020004707 nucleic acids Proteins 0.000 abstract 4
- 102000039446 nucleic acids Human genes 0.000 abstract 4
- 108091033319 polynucleotide Proteins 0.000 abstract 3
- 102000040430 polynucleotide Human genes 0.000 abstract 3
- 239000002157 polynucleotide Substances 0.000 abstract 3
- 206010061217 Infestation Diseases 0.000 abstract 2
- 230000001580 bacterial effect Effects 0.000 abstract 2
- 241000589158 Agrobacterium Species 0.000 abstract 1
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 abstract 1
- 241000555281 Brevibacillus Species 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 241000588698 Erwinia Species 0.000 abstract 1
- 241000588722 Escherichia Species 0.000 abstract 1
- 241000588748 Klebsiella Species 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 241000520272 Pantoea Species 0.000 abstract 1
- 241000589516 Pseudomonas Species 0.000 abstract 1
- 241000589180 Rhizobium Species 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/04—Insecticides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Environmental Sciences (AREA)
- Insects & Arthropods (AREA)
- Virology (AREA)
- Agronomy & Crop Science (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Dentistry (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Botany (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Se revelan proteínas pesticidas que presentan actividad tóxica contra especies de plaga Lepidopteran, e incluyen, aunque no se limitan a, TIC4029, TIC4029_1, y TIC4029_8. Se proporcionan constructos ADN que contienen una secuencia de ácido nucleico recombinante que codifica una o más de las proteínas pesticidas reveladas. Se proporcionan plantas transgénicas, células de plantas, semillas, y partes de plantas resistentes a infestación Lepidopteran infestación que contienen secuencias de ácido nucleico recombinante que codifican proteínas pesticida de la clase TIC4029. También se proporcionan métodos para detectar la presencia de las secuencias de ácido nucleico recombinante o las proteínas de la clase TIC4029 en una muestra biológica, y métodos para controlar plagas de especie Lepidopteran usando cualquiera de las proteínas pesticidas TIC4029, TIC4029_1, y TIC4029_8. Reivindicación 1: Una molécula de ácido nucleico recombinante que comprende un promotor heterólogo unido operativamente a un segmento de polinucleótidos que codifica una proteína pesticida o su fragmento, en donde, opcionalmente: a. dicha proteína pesticida comprende la secuencia de aminoácidos tal como se establece en SEQ ID Nº 2, SEQ ID Nº 4, o SEQ ID Nº 10; b. dicha proteína pesticida comprende una secuencia de aminoácidos que tiene al menos 87% o, 90%, o 95%, o 98%, o 99% o aproximadamente 100% de identidad con la secuencia de aminoácidos según se establece en SEQ ID Nº 2, SEQ ID Nº 4, o SEQ ID Nº 10; o c. dicho segmento de polinucléotidos hibrida bajo condiciones de hibridación rigurosas en un polinucleótido que tiene la secuencia de nucleótidos según se establece en SEQ ID Nº 1, SEQ ID Nº 3, SEQ ID Nº 5, SEQ ID Nº 6, SEQ ID Nº 7, SEQ ID Nº 8; o SEQ ID Nº 9. Reivindicación 3: La molécula de ácido nucleico recombinante de la reivindicación 1, presente dentro de una célula huésped, donde dicha célula huésped se selecciona del grupo integrado por una célula bacteriana y una célula vegetal. Reivindicación 4: La molécula de ácido nucleico recombinante de la reivindicación 3, donde dicha célula huésped bacteriana es de un género de bacteria que se selecciona del grupo integrado por: Agrobacterium, Rhizobium, Bacillus, Brevibacillus, Escherichia, Pseudomonas, Klebsiella, Pantoea, y Erwinia. Reivindicación 8: La molécula de ácido nucleico recombinante de la reivindicación 1, donde dicha proteína presenta actividad contra un insecto Lepidopteran. Reivindicación 24: Un método para controlar una plaga de especie Lepidopteran o infestación de plaga, dicho método comprende poner en contacto la plaga con una cantidad efectiva desde el punto de vista insecticida de: a. una proteína pesticida que comprende la secuencia de aminoácidos tal como se establece en SEQ ID Nº 2, SEQ ID Nº 4, o SEQ ID Nº 10; o b. una o más proteínas pesticidas que comprenden una secuencia de aminoácidos que tiene al menos 87% o, 90%, o 95%, o 98%, o 99% o aproximadamente 100% de identidad con la secuencia de aminoácidos según se establece en SEQ ID Nº 2, SEQ ID Nº 4, o SEQ ID Nº 10.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202063128775P | 2020-12-21 | 2020-12-21 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR124445A1 true AR124445A1 (es) | 2023-03-29 |
Family
ID=82158356
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP210103592A AR124445A1 (es) | 2020-12-21 | 2021-12-20 | Proteínas inhibidoras de insectos novedosas |
Country Status (8)
Country | Link |
---|---|
US (1) | US11825850B2 (es) |
EP (1) | EP4262400A1 (es) |
CN (1) | CN116670155A (es) |
AR (1) | AR124445A1 (es) |
AU (1) | AU2021409634A1 (es) |
CA (1) | CA3206159A1 (es) |
MX (1) | MX2023007427A (es) |
WO (1) | WO2022140227A1 (es) |
Family Cites Families (84)
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US10667524B2 (en) | 2013-09-13 | 2020-06-02 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2015120276A1 (en) | 2014-02-07 | 2015-08-13 | Pioneer Hi Bred International Inc | Insecticidal proteins and methods for their use |
CA2950947A1 (en) | 2014-06-20 | 2015-12-23 | Dow Agrosciences Llc | Vegetative insecticidal proteins useful for control of insect pests |
CA2964776A1 (en) | 2014-10-16 | 2016-04-21 | Monsanto Technology Llc | Novel chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests |
US10316329B2 (en) | 2014-10-16 | 2019-06-11 | Monsanto Technology Llc | Proteins toxic or inhibitory to lepidopteran insects |
CN106852147B (zh) | 2014-10-16 | 2021-08-27 | 孟山都技术有限公司 | 鳞翅目活性Cry1Da1氨基酸序列变异蛋白 |
US10487123B2 (en) * | 2014-10-16 | 2019-11-26 | Monsanto Technology Llc | Chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests |
MX2018002474A (es) | 2015-08-27 | 2018-06-15 | Monsanto Technology Llc | Proteinas inhibidoras de insectos novedosas. |
CA3039915A1 (en) * | 2016-10-10 | 2018-04-19 | Monsanto Technology Llc | Novel insect inhibitory proteins |
CA3229266A1 (en) | 2017-01-12 | 2018-07-19 | Monsanto Technology Llc | Pesticidal toxin proteins active against lepidopteran insects |
BR112020018654A2 (pt) | 2018-03-14 | 2020-12-29 | Pioneer Hi-Bred International, Inc. | Polipeptídeo inseticida recombinante; proteína de fusão; composição agrícola; polinucleotídeo recombinante; construto de dna; planta transgênica; método para inibir o crescimento ou exterminar uma praga de inseto ou população de praga; método para controlar os danos por praga de inseto a plantas; método para controlar a infestação de praga; e método para melhorar o rendimento de uma cultura |
EP3914608A4 (en) | 2019-01-22 | 2023-05-17 | Monsanto Technology LLC | NOVEL INSECT CONTROL PROTEINS |
UY39585A (es) * | 2020-12-23 | 2022-07-29 | Monsanto Technology Llc | Proteínas que exhiben actividad inhibidora de insectos frente a plagas con importancia agrícola de plantas de cultivo y semillas |
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2021
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- 2021-12-20 CN CN202180085702.2A patent/CN116670155A/zh active Pending
- 2021-12-20 EP EP21911957.5A patent/EP4262400A1/en active Pending
- 2021-12-20 MX MX2023007427A patent/MX2023007427A/es unknown
- 2021-12-20 US US17/556,428 patent/US11825850B2/en active Active
- 2021-12-20 WO PCT/US2021/064286 patent/WO2022140227A1/en active Application Filing
- 2021-12-20 AR ARP210103592A patent/AR124445A1/es unknown
- 2021-12-20 CA CA3206159A patent/CA3206159A1/en active Pending
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CA3206159A1 (en) | 2022-06-30 |
US20220256863A1 (en) | 2022-08-18 |
US11825850B2 (en) | 2023-11-28 |
EP4262400A1 (en) | 2023-10-25 |
CN116670155A (zh) | 2023-08-29 |
WO2022140227A1 (en) | 2022-06-30 |
MX2023007427A (es) | 2023-07-03 |
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