AR123289A1 - Procesos de cultivo celular - Google Patents
Procesos de cultivo celularInfo
- Publication number
- AR123289A1 AR123289A1 ARP210102334A ARP210102334A AR123289A1 AR 123289 A1 AR123289 A1 AR 123289A1 AR P210102334 A ARP210102334 A AR P210102334A AR P210102334 A ARP210102334 A AR P210102334A AR 123289 A1 AR123289 A1 AR 123289A1
- Authority
- AR
- Argentina
- Prior art keywords
- medium
- cell culture
- recombinant protein
- feed
- mammalian cells
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 8
- 238000004113 cell culture Methods 0.000 title abstract 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 5
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 5
- 239000012526 feed medium Substances 0.000 abstract 5
- 238000012258 culturing Methods 0.000 abstract 4
- 239000001963 growth medium Substances 0.000 abstract 4
- 210000004962 mammalian cell Anatomy 0.000 abstract 4
- 238000004519 manufacturing process Methods 0.000 abstract 3
- 230000001502 supplementing effect Effects 0.000 abstract 3
- 239000002609 medium Substances 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Reivindicación 1: Un proceso para cultivar células de mamíferos que expresan una proteína recombinante, caracterizado porque el proceso comprende los pasos de cultivar las células de mamíferos en un medio de cultivo y complementar el cultivo celular durante una fase de producción con al menos un medio de alimentación, en donde el pH de este en al menos un medio de alimentación es de aproximadamente 5,0 a aproximadamente 6,3. Reivindicación 2: Un proceso para producir una proteína recombinante, caracterizado porque el proceso comprende los pasos de cultivar células de mamífero que expresan dicha proteína recombinante en un medio de cultivo, complementando el cultivo celular durante una fase de producción con al menos un medio de alimentación, en donde el pH de esta en al menos un medio de alimentación es de aproximadamente 5,0 a aproximadamente 6,3. Reivindicación 3: Un proceso para reducir o prevenir la precipitación en un medio de alimentación, caracterizado porque el proceso comprende los pasos de cultivar células de mamíferos que expresan una proteína recombinante en un medio de cultivo y complementar el cultivo celular durante una fase de producción con al menos un medio de alimentación, en el que el pH de este al menos un medio de alimentación es de aproximadamente 5,0 a aproximadamente 6,3. Reivindicación 14: Una proteína recombinante, caracterizada porque se produce por el proceso de acuerdo con cualquiera de las reivindicaciones 1 a 3 ó 5 a 10.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB2012991.2A GB202012991D0 (en) | 2020-08-20 | 2020-08-20 | Cell culture processes |
Publications (1)
Publication Number | Publication Date |
---|---|
AR123289A1 true AR123289A1 (es) | 2022-11-16 |
Family
ID=72660828
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP210102334A AR123289A1 (es) | 2020-08-20 | 2021-08-19 | Procesos de cultivo celular |
Country Status (13)
Country | Link |
---|---|
US (1) | US20230348850A1 (es) |
EP (1) | EP4200432A1 (es) |
JP (1) | JP2023538581A (es) |
KR (1) | KR20230051277A (es) |
CN (1) | CN115916814A (es) |
AR (1) | AR123289A1 (es) |
AU (1) | AU2021329034A1 (es) |
BR (1) | BR112023002377A2 (es) |
CA (1) | CA3190220A1 (es) |
GB (1) | GB202012991D0 (es) |
IL (1) | IL300509A (es) |
MX (1) | MX2023002054A (es) |
WO (1) | WO2022038250A1 (es) |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2243827B2 (en) | 1996-08-30 | 2017-11-22 | Life Technologies Corporation | Serum-free mammalian cell culture medium, and uses thereof |
WO2008013809A1 (en) | 2006-07-24 | 2008-01-31 | Amgen Inc. | Cell culture methods |
CA2687082C (en) | 2007-05-11 | 2014-01-14 | Amgen Inc. | Improved feed media |
US20110262965A1 (en) | 2010-04-23 | 2011-10-27 | Life Technologies Corporation | Cell culture medium comprising small peptides |
EP3330370B1 (en) | 2010-04-26 | 2021-02-24 | Novartis AG | Process for cultivation of cho cells |
WO2013158275A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Cell culture methods to reduce acidic species |
US20130281355A1 (en) | 2012-04-24 | 2013-10-24 | Genentech, Inc. | Cell culture compositions and methods for polypeptide production |
GB201411320D0 (en) | 2014-06-25 | 2014-08-06 | Ucb Biopharma Sprl | Antibody construct |
-
2020
- 2020-08-20 GB GBGB2012991.2A patent/GB202012991D0/en not_active Ceased
-
2021
- 2021-08-19 AR ARP210102334A patent/AR123289A1/es unknown
- 2021-08-19 MX MX2023002054A patent/MX2023002054A/es unknown
- 2021-08-19 CA CA3190220A patent/CA3190220A1/en active Pending
- 2021-08-19 EP EP21765907.7A patent/EP4200432A1/en active Pending
- 2021-08-19 BR BR112023002377A patent/BR112023002377A2/pt unknown
- 2021-08-19 AU AU2021329034A patent/AU2021329034A1/en active Pending
- 2021-08-19 JP JP2023512002A patent/JP2023538581A/ja active Pending
- 2021-08-19 KR KR1020237009032A patent/KR20230051277A/ko unknown
- 2021-08-19 IL IL300509A patent/IL300509A/en unknown
- 2021-08-19 CN CN202180051162.6A patent/CN115916814A/zh active Pending
- 2021-08-19 WO PCT/EP2021/073094 patent/WO2022038250A1/en active Application Filing
- 2021-08-19 US US18/021,820 patent/US20230348850A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CA3190220A1 (en) | 2022-02-24 |
EP4200432A1 (en) | 2023-06-28 |
JP2023538581A (ja) | 2023-09-08 |
US20230348850A1 (en) | 2023-11-02 |
WO2022038250A1 (en) | 2022-02-24 |
KR20230051277A (ko) | 2023-04-17 |
AU2021329034A1 (en) | 2023-03-09 |
BR112023002377A2 (pt) | 2023-03-21 |
MX2023002054A (es) | 2023-03-16 |
CN115916814A (zh) | 2023-04-04 |
GB202012991D0 (en) | 2020-10-07 |
IL300509A (en) | 2023-04-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CY1118734T1 (el) | Μεθοδοι παρασκευης γλυκοπρωτεϊνων σε κυτταροκαλλιεργειες θηλαστικου χρησιμοποιωντας γλυκοκορτικοειδη | |
MX2018002121A (es) | Elaboracion de fosfatasas alcalinas. | |
AR110864A2 (es) | Procesos para cultivos celulares | |
DE60335024D1 (de) | Produktqualitätsverbesserung in säugerzellkulturverfahrenzur proteinproduktion | |
AR095660A1 (es) | Métodos de fabricación para el control el contenido de lisina c-terminal, galactosa y ácido siálico en proteínas recombinantes | |
CA2797356A1 (en) | Improved cell culture medium | |
IL177493A (en) | Use of a serum-free cell culture medium for the production of il-18bp | |
WO2011062926A3 (en) | Methods for enhanced protein production | |
NZ598677A (en) | Production of proteins in glutamine-free cell culture media | |
US11104726B2 (en) | Methods of replicating a large scale eculizumab production cell culture | |
AR073113A1 (es) | Produccion de metionina sin n- acil -metionina | |
EP1505149A4 (en) | METHYLOTROPHER PRODUCING A SUGAR CHAIN OF THE MAMMAL TYPE | |
CL2020001536A1 (es) | Procedimiento de fabricación continuo para productos de anticuerpos biespecíficos. | |
AR086200A1 (es) | Hongos filamentosos que tienen un fenotipo de viscosidad alterado | |
PE20221184A1 (es) | Metodos de produccion de carne por cultivo celular in vitro | |
AR123289A1 (es) | Procesos de cultivo celular | |
AR123609A1 (es) | Líneas de células de mamífero con desactivación génica | |
RU2018130530A (ru) | Способ получения активного фактора роста гепатоцитов (hgf) | |
MX2020013633A (es) | Metodos para producir proteinas recombinantes. | |
AR118617A1 (es) | Medios de cultivo celular que comprenden cetoácidos | |
CN103525750B (zh) | 培养基及其应用 | |
BRPI0517942A (pt) | meio de cultura de células isento de soro para células de mamìferos | |
MX2018007025A (es) | Metodo de producción masiva del rotífero proales similis (genbank km078762, variedad mexicana). | |
BRPI0411125A (pt) | processo para a produção de células de tecido partindo de células-tronco pluripotentes derivadas de células epiteliais pigmentadas de ìris de animal e células de tecido obtidas pelo processo | |
AR125343A1 (es) | Procedimientos de cultivo celular |