AR123289A1 - Procesos de cultivo celular - Google Patents

Procesos de cultivo celular

Info

Publication number
AR123289A1
AR123289A1 ARP210102334A ARP210102334A AR123289A1 AR 123289 A1 AR123289 A1 AR 123289A1 AR P210102334 A ARP210102334 A AR P210102334A AR P210102334 A ARP210102334 A AR P210102334A AR 123289 A1 AR123289 A1 AR 123289A1
Authority
AR
Argentina
Prior art keywords
medium
cell culture
recombinant protein
feed
mammalian cells
Prior art date
Application number
ARP210102334A
Other languages
English (en)
Inventor
Yahia Bassem Ben
Antoine Philippe Thomas Piednoir
Original Assignee
UCB Biopharma SRL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UCB Biopharma SRL filed Critical UCB Biopharma SRL
Publication of AR123289A1 publication Critical patent/AR123289A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Reivindicación 1: Un proceso para cultivar células de mamíferos que expresan una proteína recombinante, caracterizado porque el proceso comprende los pasos de cultivar las células de mamíferos en un medio de cultivo y complementar el cultivo celular durante una fase de producción con al menos un medio de alimentación, en donde el pH de este en al menos un medio de alimentación es de aproximadamente 5,0 a aproximadamente 6,3. Reivindicación 2: Un proceso para producir una proteína recombinante, caracterizado porque el proceso comprende los pasos de cultivar células de mamífero que expresan dicha proteína recombinante en un medio de cultivo, complementando el cultivo celular durante una fase de producción con al menos un medio de alimentación, en donde el pH de esta en al menos un medio de alimentación es de aproximadamente 5,0 a aproximadamente 6,3. Reivindicación 3: Un proceso para reducir o prevenir la precipitación en un medio de alimentación, caracterizado porque el proceso comprende los pasos de cultivar células de mamíferos que expresan una proteína recombinante en un medio de cultivo y complementar el cultivo celular durante una fase de producción con al menos un medio de alimentación, en el que el pH de este al menos un medio de alimentación es de aproximadamente 5,0 a aproximadamente 6,3. Reivindicación 14: Una proteína recombinante, caracterizada porque se produce por el proceso de acuerdo con cualquiera de las reivindicaciones 1 a 3 ó 5 a 10.
ARP210102334A 2020-08-20 2021-08-19 Procesos de cultivo celular AR123289A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB2012991.2A GB202012991D0 (en) 2020-08-20 2020-08-20 Cell culture processes

Publications (1)

Publication Number Publication Date
AR123289A1 true AR123289A1 (es) 2022-11-16

Family

ID=72660828

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP210102334A AR123289A1 (es) 2020-08-20 2021-08-19 Procesos de cultivo celular

Country Status (13)

Country Link
US (1) US20230348850A1 (es)
EP (1) EP4200432A1 (es)
JP (1) JP2023538581A (es)
KR (1) KR20230051277A (es)
CN (1) CN115916814A (es)
AR (1) AR123289A1 (es)
AU (1) AU2021329034A1 (es)
BR (1) BR112023002377A2 (es)
CA (1) CA3190220A1 (es)
GB (1) GB202012991D0 (es)
IL (1) IL300509A (es)
MX (1) MX2023002054A (es)
WO (1) WO2022038250A1 (es)

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2243827B2 (en) 1996-08-30 2017-11-22 Life Technologies Corporation Serum-free mammalian cell culture medium, and uses thereof
WO2008013809A1 (en) 2006-07-24 2008-01-31 Amgen Inc. Cell culture methods
CA2687082C (en) 2007-05-11 2014-01-14 Amgen Inc. Improved feed media
US20110262965A1 (en) 2010-04-23 2011-10-27 Life Technologies Corporation Cell culture medium comprising small peptides
EP3330370B1 (en) 2010-04-26 2021-02-24 Novartis AG Process for cultivation of cho cells
WO2013158275A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Cell culture methods to reduce acidic species
US20130281355A1 (en) 2012-04-24 2013-10-24 Genentech, Inc. Cell culture compositions and methods for polypeptide production
GB201411320D0 (en) 2014-06-25 2014-08-06 Ucb Biopharma Sprl Antibody construct

Also Published As

Publication number Publication date
CA3190220A1 (en) 2022-02-24
EP4200432A1 (en) 2023-06-28
JP2023538581A (ja) 2023-09-08
US20230348850A1 (en) 2023-11-02
WO2022038250A1 (en) 2022-02-24
KR20230051277A (ko) 2023-04-17
AU2021329034A1 (en) 2023-03-09
BR112023002377A2 (pt) 2023-03-21
MX2023002054A (es) 2023-03-16
CN115916814A (zh) 2023-04-04
GB202012991D0 (en) 2020-10-07
IL300509A (en) 2023-04-01

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