AR084757A1 - Procedimiento de inmovilizacion de ligandos nucleicos - Google Patents

Procedimiento de inmovilizacion de ligandos nucleicos

Info

Publication number
AR084757A1
AR084757A1 ARP110105039A ARP110105039A AR084757A1 AR 084757 A1 AR084757 A1 AR 084757A1 AR P110105039 A ARP110105039 A AR P110105039A AR P110105039 A ARP110105039 A AR P110105039A AR 084757 A1 AR084757 A1 AR 084757A1
Authority
AR
Argentina
Prior art keywords
target molecule
support
complex
nucleic
immobilization
Prior art date
Application number
ARP110105039A
Other languages
English (en)
Inventor
Gerald Perret
Egisto Boscheti
Original Assignee
Lfb Biotechnologies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR1061366A external-priority patent/FR2970003B1/fr
Priority claimed from FR1159604A external-priority patent/FR2981651B1/fr
Application filed by Lfb Biotechnologies filed Critical Lfb Biotechnologies
Publication of AR084757A1 publication Critical patent/AR084757A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/289—Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204—Inorganic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3278—Polymers being grafted on the carrier
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Inorganic Chemistry (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

Reivindicación 1: Procedimiento de inmovilización de ligandos nucleicos que comprenden, al menos, una función amina primaria, por injerto sobre un soporte sólido que presenta grupos ácidos carboxílicos activados, que comprenden una etapa de acoplamiento covalente de dichos ácidos nucleicos sobre dicho soporte sólido a un pH inferior a 6.Reivindicación 13: Método de preparación de un soporte de afinidad que comprende la implementación del procedimiento de inmovilización tal como se ha definido en una cualquiera de las reivindicaciones 1 a 12. Reivindicación 19: Complejo resultante de la interacción de un ligando nucleico y una molécula diana, donde dicho complejo está formado en la superficie de un soporte sólido tal como se ha definido en una cualquiera de las reivindicaciones 14 a 18. Reivindicación 20: Utilización de un soporte de afinidad tal como se ha definido en una cualquiera de las reivindicaciones 14 a 18 para la purificación de una molécula diana. Reivindicación 22: Procedimiento de purificación de una molécula diana con un soporte de afinidad tal como se ha definido de acuerdo con una cualquiera de las reivindicaciones 14 a 18, que comprende las etapas siguientes: a) poner en contacto una composición por purificar que comprende la molécula diana de interés con un soporte sólido de afinidad tal como se ha definido en una de las reivindicaciones 14 a 18, con el objeto de formar un complejo entre (i) ligandos nucleicos injertados sobre dicho soporte y (ii) dicha molécula diana, y b) liberar dicha molécula diana a partir del complejo formado en la etapa a), y recuperar dicha molécula diana purificada.
ARP110105039A 2010-12-30 2011-12-30 Procedimiento de inmovilizacion de ligandos nucleicos AR084757A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1061366A FR2970003B1 (fr) 2010-12-30 2010-12-30 Procede d'immobilisation d'acides nucleiques
FR1159604A FR2981651B1 (fr) 2011-10-24 2011-10-24 Procede d'immobilisation de ligands nucleiques.

Publications (1)

Publication Number Publication Date
AR084757A1 true AR084757A1 (es) 2013-06-05

Family

ID=45531495

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110105039A AR084757A1 (es) 2010-12-30 2011-12-30 Procedimiento de inmovilizacion de ligandos nucleicos

Country Status (10)

Country Link
US (1) US9803184B2 (es)
EP (1) EP2658864A1 (es)
JP (1) JP6178727B2 (es)
KR (1) KR20140057187A (es)
CN (1) CN103403015A (es)
AR (1) AR084757A1 (es)
AU (1) AU2011350672B2 (es)
BR (1) BR112013016704A2 (es)
CA (1) CA2823030A1 (es)
WO (1) WO2012090183A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112013016704A2 (pt) 2010-12-30 2017-03-14 Laboratoire Français Du Fractionnement Et Des Biotechnologies método para imobilizar os ligandos nucléicos.
FR3011250A1 (fr) 2013-09-30 2015-04-03 Lab Francais Du Fractionnement Acides nucleiques se liant specifiquement au facteur ix/ixa humain, et utilisations
FR3021330A1 (fr) 2014-05-20 2015-11-27 Lab Francais Du Fractionnement Procede de dosage d'aptameres adn
GB201503578D0 (en) * 2015-03-03 2015-04-15 Ge Healthcare Bio Sciences Ab Sanitization method for affinity chromatography matrices
PT109480B (pt) * 2016-06-22 2020-03-13 Inst Superior Tecnico Polímero de polibenzimidazolo com cadeia espaçadora funcionalizada e seu método de obtenção para remoção de impurezas genotóxicas
WO2018007767A1 (fr) 2016-07-06 2018-01-11 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Fibrinogène liquide stable
EP3491136A1 (en) 2016-07-28 2019-06-05 Laboratoire Francais du Fractionnement et des Biotechnologies Societe Anonyme Anti- immunoglobulin g aptamers and uses thereof
CA3031386A1 (en) * 2016-07-28 2018-02-01 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Method for obtaining aptamers
CN106512958B (zh) * 2016-11-22 2019-03-08 华南师范大学 一种核酸适配体修饰壳聚糖纳米纤维的制备方法及应用
WO2018109213A1 (en) 2016-12-16 2018-06-21 Laboratoire Français Du Fractionnement Et Des Biotechnologies Aptamers directed against a kappa light chain-containing protein and uses thereof
GB201714563D0 (en) * 2017-09-11 2017-10-25 Life Tech As Coupling methods and compositions
US11598769B2 (en) * 2018-07-27 2023-03-07 Massachusetts Institute Of Technology Oligomerized protein-polymer conjugates
CN109806852B (zh) * 2019-03-04 2020-08-07 北京理工大学 一种腺嘌呤功能化聚丙二醇的应用
WO2023084369A1 (en) * 2021-11-12 2023-05-19 3M Innovative Properties Company Method of processing polynucleic acids

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DK641487A (da) * 1987-12-07 1989-06-08 Gluetech Aps Fremgangsmaade til modificering af polymeroverflader
US5215882A (en) * 1989-11-30 1993-06-01 Ortho Diagnostic Systems, Inc. Method of immobilizing nucleic acid on a solid surface for use in nucleic acid hybridization assays
US5660985A (en) 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US5756291A (en) * 1992-08-21 1998-05-26 Gilead Sciences, Inc. Aptamers specific for biomolecules and methods of making
US6037124A (en) * 1996-09-27 2000-03-14 Beckman Coulter, Inc. Carboxylated polyvinylidene fluoride solid supports for the immobilization of biomolecules and methods of use thereof
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BR112013016704A2 (pt) 2010-12-30 2017-03-14 Laboratoire Français Du Fractionnement Et Des Biotechnologies método para imobilizar os ligandos nucléicos.

Also Published As

Publication number Publication date
WO2012090183A1 (fr) 2012-07-05
EP2658864A1 (fr) 2013-11-06
CN103403015A (zh) 2013-11-20
KR20140057187A (ko) 2014-05-12
US20130344567A1 (en) 2013-12-26
CA2823030A1 (fr) 2012-07-05
JP2014505867A (ja) 2014-03-06
AU2011350672A1 (en) 2013-05-02
BR112013016704A2 (pt) 2017-03-14
JP6178727B2 (ja) 2017-08-09
US9803184B2 (en) 2017-10-31
AU2011350672B2 (en) 2016-06-02

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