AR029762A1 - EXPRESSION CASSETTE; RECOMBINING VECTOR THAT UNDERSTANDS IT; MICROORGANISM THAT UNDERSTANDS THIS LAST; USE OF THE VECTOR OR MICROORGANISM TO TRANSFORM A PLANT; MODIFIED TRANSGENIC PLANT; METHOD OF GENERATION OF TRANSGENIC PLANTS; USE OF EXPRESSION CASSETTE, VECTOR, MICROORGANISM OR - Google Patents

EXPRESSION CASSETTE; RECOMBINING VECTOR THAT UNDERSTANDS IT; MICROORGANISM THAT UNDERSTANDS THIS LAST; USE OF THE VECTOR OR MICROORGANISM TO TRANSFORM A PLANT; MODIFIED TRANSGENIC PLANT; METHOD OF GENERATION OF TRANSGENIC PLANTS; USE OF EXPRESSION CASSETTE, VECTOR, MICROORGANISM OR

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Publication number
AR029762A1
AR029762A1 ARP000103954A ARP000103954A AR029762A1 AR 029762 A1 AR029762 A1 AR 029762A1 AR P000103954 A ARP000103954 A AR P000103954A AR P000103954 A ARP000103954 A AR P000103954A AR 029762 A1 AR029762 A1 AR 029762A1
Authority
AR
Argentina
Prior art keywords
microorganism
vector
plant
expression cassette
plants
Prior art date
Application number
ARP000103954A
Other languages
Spanish (es)
Inventor
Marcus Ebneth
Karin Herbers
Michael Geiger
Isolde Saalbach
Original Assignee
Sungene Gmbh & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sungene Gmbh & Co Kgaa filed Critical Sungene Gmbh & Co Kgaa
Publication of AR029762A1 publication Critical patent/AR029762A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un cassette de expresion que comprende: a) la secuencia de ácidos nucleicos de codificacion para 4-hidroxifenilpiruvato dioxigenasa (HPPD) o para un equivalente funcional de la misma; y/o b) al menos una secuencia HPPD de codificacion se enlaza funcionalmente a la secuencia de codificacion de un péptido de tránsito específico del organelo de una planta. Un vector recombinante que comprende al menos un cassette de expresion de acuerdo con lo descripto. Un microorganismo que comprende dicho vector recombinante y que pertenece al género Agrobacterium y en particular a la especie Agrobacterium tumefaiens. Uso del vector o de un microorganismo de acuerdo con lo descripto para la transformacion de plantas, células de plantas, tejido de plantas u organos de plantas para mejorar la síntesis de tocoferol. Las plantas se seleccionan entre los cultivos tales como cereales, maíz, sojas, arroz, algodon, cana de azucar, canola, girasoles, lino, papas, tabaco, tomates, colza, alfalfa, especies para ensaladas, como por ejemplo berro, y las distintas especies de árboles, nueces y vides. Un método de generacion de plantas transgénicas en donde las células de plantas, tejido u organos o protoplastos son transformados con un vector con un microorganismo de acuerdo a lo descripto. Uso de un cassette de expresion, un vector un microorganismo segun lo descripto para obtener una planta transgénica, de donde se obtienen metabolitos de plantas, en particular tocoferoles. Un proceso de preparacion de tocoferoles que comprende aislamiento del tocoferol de un cultivo de una planta transformada segun lo descripto.An expression cassette comprising: a) the nucleic acid sequence encoding for 4-hydroxyphenylpyruvate dioxygenase (HPPD) or for a functional equivalent thereof; and / or b) at least one HPPD coding sequence is functionally linked to the coding sequence of a specific transit peptide from the organelle of a plant. A recombinant vector comprising at least one expression cassette as described. A microorganism comprising said recombinant vector and belonging to the genus Agrobacterium and in particular to the species Agrobacterium tumefaiens. Use of the vector or a microorganism in accordance with what is described for the transformation of plants, plant cells, plant tissue or plant organs to improve the synthesis of tocopherol. Plants are selected from crops such as cereals, corn, soybeans, rice, cotton, sugar cane, canola, sunflowers, flax, potatoes, tobacco, tomatoes, rapeseed, alfalfa, salad species, such as cress, and different species of trees, nuts and vines. A method of generating transgenic plants where the cells of plants, tissue or organs or protoplasts are transformed with a vector with a microorganism as described. Using an expression cassette, a vector a microorganism as described to obtain a transgenic plant, from which plant metabolites are obtained, in particular tocopherols. A tocopherol preparation process comprising tocopherol isolation from a crop of a transformed plant as described.

ARP000103954A 1999-08-11 2000-07-31 EXPRESSION CASSETTE; RECOMBINING VECTOR THAT UNDERSTANDS IT; MICROORGANISM THAT UNDERSTANDS THIS LAST; USE OF THE VECTOR OR MICROORGANISM TO TRANSFORM A PLANT; MODIFIED TRANSGENIC PLANT; METHOD OF GENERATION OF TRANSGENIC PLANTS; USE OF EXPRESSION CASSETTE, VECTOR, MICROORGANISM OR AR029762A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19937957A DE19937957A1 (en) 1999-08-11 1999-08-11 Homogenate dioxygenase

Publications (1)

Publication Number Publication Date
AR029762A1 true AR029762A1 (en) 2003-07-16

Family

ID=7917993

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP000103954A AR029762A1 (en) 1999-08-11 2000-07-31 EXPRESSION CASSETTE; RECOMBINING VECTOR THAT UNDERSTANDS IT; MICROORGANISM THAT UNDERSTANDS THIS LAST; USE OF THE VECTOR OR MICROORGANISM TO TRANSFORM A PLANT; MODIFIED TRANSGENIC PLANT; METHOD OF GENERATION OF TRANSGENIC PLANTS; USE OF EXPRESSION CASSETTE, VECTOR, MICROORGANISM OR

Country Status (7)

Country Link
EP (1) EP1200598A2 (en)
AR (1) AR029762A1 (en)
AU (1) AU6440900A (en)
BR (1) BR0013127A (en)
CA (1) CA2381316A1 (en)
DE (1) DE19937957A1 (en)
WO (1) WO2001012827A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2253220T3 (en) 1999-04-15 2006-06-01 Calgene Llc SEQUENCES OF NUCLEIC ACID OF PROTEINS INVOLVED IN THE SYNTHESIS OF TOCOFEROL.
US6872815B1 (en) 2000-10-14 2005-03-29 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
DE60140312D1 (en) 2000-08-07 2009-12-10 Monsanto Technology Llc Am methyl-d-erythriol phosphat reaktionsweg beteiligte gene
DE10046462A1 (en) * 2000-09-19 2002-05-29 Sungene Gmbh & Co Kgaa Improved procedures for vitamin E biosynthesis
US7161061B2 (en) 2001-05-09 2007-01-09 Monsanto Technology Llc Metabolite transporters
DE60230608D1 (en) 2001-05-09 2009-02-12 Monsanto Technology Llc TYRA GENES AND ITS USE
EP1427832A4 (en) 2001-08-17 2006-09-06 Monsanto Technology Llc Methyltransferase genes and uses thereof
AU2002342114B2 (en) 2001-10-25 2008-07-31 Monsanto Technology Llc Aromatic methyltransferases and uses thereof
BRPI0308740B1 (en) 2002-03-19 2018-11-21 Monsanto Technology Llc nucleic acid molecule encoding a homogenous prenyl transferase (“hpt”) and method of producing plant having increased tocopherol level seed
US7230165B2 (en) 2002-08-05 2007-06-12 Monsanto Technology Llc Tocopherol biosynthesis related genes and uses thereof
GB2595633A (en) * 2020-04-15 2021-12-08 Univ Liverpool Anti-infective agents

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA199900150A1 (en) * 1996-07-25 2000-04-24 Америкэн Сиэнамид Компани HPPD gene and inhibitors
DE19730066A1 (en) * 1997-07-14 1999-01-21 Basf Ag DNA sequence coding for a hydroxyphenylpyruvate dioxygenase and its overproduction in plants
FR2778527A1 (en) * 1998-05-18 1999-11-19 Rhone Poulenc Agrochimie Producing tomato, cereal or oilseed plants, especially maize, oilseed rape, soya or sunflower plants, from which tocopherols can be extracted and purified
CA2339519A1 (en) * 1998-08-05 2000-02-17 Sungene Gmbh & Co.Kgaa Dna sequence coding for a 1-deoxy-d-xylulose-5-phosphate synthase and the overproduction thereof in plants

Also Published As

Publication number Publication date
DE19937957A1 (en) 2001-02-15
AU6440900A (en) 2001-03-13
BR0013127A (en) 2002-04-23
WO2001012827A9 (en) 2002-05-10
WO2001012827A3 (en) 2001-08-23
WO2001012827A2 (en) 2001-02-22
CA2381316A1 (en) 2001-02-22
EP1200598A2 (en) 2002-05-02

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