AR029762A1 - Cassette de expresion; vector recombinante que lo comprende; microorganismo que comprende este ultimo; uso del vector o del microorganismo para transformar una planta; planta transgenica modificada; metodo de generacion de plantas transgenicas; uso del cassette de expresion, vector, microorganismo o - Google Patents

Cassette de expresion; vector recombinante que lo comprende; microorganismo que comprende este ultimo; uso del vector o del microorganismo para transformar una planta; planta transgenica modificada; metodo de generacion de plantas transgenicas; uso del cassette de expresion, vector, microorganismo o

Info

Publication number
AR029762A1
AR029762A1 ARP000103954A ARP000103954A AR029762A1 AR 029762 A1 AR029762 A1 AR 029762A1 AR P000103954 A ARP000103954 A AR P000103954A AR P000103954 A ARP000103954 A AR P000103954A AR 029762 A1 AR029762 A1 AR 029762A1
Authority
AR
Argentina
Prior art keywords
microorganism
vector
plant
expression cassette
plants
Prior art date
Application number
ARP000103954A
Other languages
English (en)
Inventor
Marcus Ebneth
Karin Herbers
Michael Geiger
Isolde Saalbach
Original Assignee
Sungene Gmbh & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sungene Gmbh & Co Kgaa filed Critical Sungene Gmbh & Co Kgaa
Publication of AR029762A1 publication Critical patent/AR029762A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un cassette de expresion que comprende: a) la secuencia de ácidos nucleicos de codificacion para 4-hidroxifenilpiruvato dioxigenasa (HPPD) o para un equivalente funcional de la misma; y/o b) al menos una secuencia HPPD de codificacion se enlaza funcionalmente a la secuencia de codificacion de un péptido de tránsito específico del organelo de una planta. Un vector recombinante que comprende al menos un cassette de expresion de acuerdo con lo descripto. Un microorganismo que comprende dicho vector recombinante y que pertenece al género Agrobacterium y en particular a la especie Agrobacterium tumefaiens. Uso del vector o de un microorganismo de acuerdo con lo descripto para la transformacion de plantas, células de plantas, tejido de plantas u organos de plantas para mejorar la síntesis de tocoferol. Las plantas se seleccionan entre los cultivos tales como cereales, maíz, sojas, arroz, algodon, cana de azucar, canola, girasoles, lino, papas, tabaco, tomates, colza, alfalfa, especies para ensaladas, como por ejemplo berro, y las distintas especies de árboles, nueces y vides. Un método de generacion de plantas transgénicas en donde las células de plantas, tejido u organos o protoplastos son transformados con un vector con un microorganismo de acuerdo a lo descripto. Uso de un cassette de expresion, un vector un microorganismo segun lo descripto para obtener una planta transgénica, de donde se obtienen metabolitos de plantas, en particular tocoferoles. Un proceso de preparacion de tocoferoles que comprende aislamiento del tocoferol de un cultivo de una planta transformada segun lo descripto.
ARP000103954A 1999-08-11 2000-07-31 Cassette de expresion; vector recombinante que lo comprende; microorganismo que comprende este ultimo; uso del vector o del microorganismo para transformar una planta; planta transgenica modificada; metodo de generacion de plantas transgenicas; uso del cassette de expresion, vector, microorganismo o AR029762A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19937957A DE19937957A1 (de) 1999-08-11 1999-08-11 Homogentisat-Dioxygenase

Publications (1)

Publication Number Publication Date
AR029762A1 true AR029762A1 (es) 2003-07-16

Family

ID=7917993

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP000103954A AR029762A1 (es) 1999-08-11 2000-07-31 Cassette de expresion; vector recombinante que lo comprende; microorganismo que comprende este ultimo; uso del vector o del microorganismo para transformar una planta; planta transgenica modificada; metodo de generacion de plantas transgenicas; uso del cassette de expresion, vector, microorganismo o

Country Status (7)

Country Link
EP (1) EP1200598A2 (es)
AR (1) AR029762A1 (es)
AU (1) AU6440900A (es)
BR (1) BR0013127A (es)
CA (1) CA2381316A1 (es)
DE (1) DE19937957A1 (es)
WO (1) WO2001012827A2 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE316578T1 (de) 1999-04-15 2006-02-15 Calgene Llc Nukleinsäuresequenzen für proteine, die an der tocopherolbiosynthese beteiligt sind
US6872815B1 (en) 2000-10-14 2005-03-29 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
AU2001290522B2 (en) 2000-08-07 2006-11-30 Monsanto Technology Llc Methyl-D-erythritol phosphate pathway genes
DE10046462A1 (de) * 2000-09-19 2002-05-29 Sungene Gmbh & Co Kgaa Verbesserte Verfahren zur Vitamin E Biosynthese
US7161061B2 (en) 2001-05-09 2007-01-09 Monsanto Technology Llc Metabolite transporters
DE60230608D1 (de) 2001-05-09 2009-02-12 Monsanto Technology Llc Tyra-gene und ihre verwendung
US7244877B2 (en) 2001-08-17 2007-07-17 Monsanto Technology Llc Methyltransferase from cotton and uses thereof
EP1444348B1 (en) 2001-10-25 2010-01-27 Monsanto Technology LLC Aromatic methyltransferases and uses thereof
WO2003080647A2 (en) 2002-03-19 2003-10-02 Monsanto Technology, Llc Homogentisate prenyl transferase ('hpt') nucleic acids and polypeptides, and uses thereof
US7230165B2 (en) 2002-08-05 2007-06-12 Monsanto Technology Llc Tocopherol biosynthesis related genes and uses thereof
GB2595633A (en) * 2020-04-15 2021-12-08 Univ Liverpool Anti-infective agents

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EE9900030A (et) * 1996-07-25 1999-08-16 American Cyanamid Company HPPD geen ja inhibiitorid
DE19730066A1 (de) * 1997-07-14 1999-01-21 Basf Ag DNA-Sequenz codierend für eine Hydroxyphenylpyruvatdioxygenase und deren Überproduktion in Pflanzen
FR2778527A1 (fr) * 1998-05-18 1999-11-19 Rhone Poulenc Agrochimie Nouvelle methode de production de tocopherols dans les plantes et plantes obtenues
CA2339519A1 (en) * 1998-08-05 2000-02-17 Sungene Gmbh & Co.Kgaa Dna sequence coding for a 1-deoxy-d-xylulose-5-phosphate synthase and the overproduction thereof in plants

Also Published As

Publication number Publication date
AU6440900A (en) 2001-03-13
WO2001012827A3 (de) 2001-08-23
EP1200598A2 (de) 2002-05-02
CA2381316A1 (en) 2001-02-22
WO2001012827A2 (de) 2001-02-22
BR0013127A (pt) 2002-04-23
DE19937957A1 (de) 2001-02-15
WO2001012827A9 (de) 2002-05-10

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