BR0013127A - Expression cassette, recombinant vector, microorganism, transgenic plant, method for generating transgenic plants, uses of a microorganism vector, an expression cassette, or a transgenic plant, and process for the preparation of tocopherols - Google Patents

Expression cassette, recombinant vector, microorganism, transgenic plant, method for generating transgenic plants, uses of a microorganism vector, an expression cassette, or a transgenic plant, and process for the preparation of tocopherols

Info

Publication number
BR0013127A
BR0013127A BR0013127-0A BR0013127A BR0013127A BR 0013127 A BR0013127 A BR 0013127A BR 0013127 A BR0013127 A BR 0013127A BR 0013127 A BR0013127 A BR 0013127A
Authority
BR
Brazil
Prior art keywords
microorganism
expression cassette
transgenic plant
vector
protection
Prior art date
Application number
BR0013127-0A
Other languages
Portuguese (pt)
Inventor
Marcus Ebneth
Karin Herbers
Michael Geiger
Isolde Saalbach
Original Assignee
Sungene Gmgh & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sungene Gmgh & Co Kgaa filed Critical Sungene Gmgh & Co Kgaa
Publication of BR0013127A publication Critical patent/BR0013127A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"CASSETE DE EXPRESSãO, VETOR RECOMBINANTE, MICROORGANISMO, PLANTA TRANSGêNICA, MéTODO PARA GERAR PLANTAS TRANSGêNICAS, USOS DE UM VETOR, DE UM MICROORGANISMO, DE UM CASSETE DE EXPRESSãO, OU DE UMA PLANTA TRANSGêNICA, E, PROCESSO PARA A PREPARAçãO DE TOCOFERóIS". A invenção refere-se a um novo tipo de cassetes de expressão que, sob controle genético, contém seq³ências de ácido nucleico reguladoras a) seq³ência de ácido nucleico codificadora de 4-hidro-fenil-piruvato-dioxigenase (HPPD) ou de um de seus equivalentes funcionais; e/ou b) pelo menos uma seq³ência de ácido nucleico (anti-HGD), que pode inibir a atividade de homogentisato-dioxigenase (HGD). A invenção também se refere aos vetores que são adequados para a produção de plantas possuindo um conteúdo de tocoferol aumentado, às plantas transgênicas produzidas com os mesmos, e a um método para a produção de plantas transgênicas possuindo um conteúdo de tocoferol aumentado."EXPRESSION CASSETTE, RECOMBINANT VECTOR, MICROORGANISM, TRANSGENIC PLANT, METHOD FOR GENERATING TRANSGENIC PLANTS, USES OF A VECTOR, A MICROORGANISM, AN EXPRESSION CASSETTE, OR A TRANSGENIC PLANT FOR PROTECTION, AND PROCEDURE FOR PROTECTION, AND PROCEDURE FOR PROTECTION AND PROTECTION. The invention relates to a new type of expression cassettes that, under genetic control, contain regulatory nucleic acid sequences a) nucleic acid sequence encoding 4-hydro-phenyl-pyruvate-dioxigenase (HPPD) or one of its components functional equivalents; and / or b) at least one nucleic acid (anti-HGD) sequence, which can inhibit homogentisate-dioxigenase (HGD) activity. The invention also relates to vectors that are suitable for the production of plants having an increased tocopherol content, to transgenic plants produced therefrom, and to a method for the production of transgenic plants having an increased tocopherol content.

BR0013127-0A 1999-08-11 2000-08-10 Expression cassette, recombinant vector, microorganism, transgenic plant, method for generating transgenic plants, uses of a microorganism vector, an expression cassette, or a transgenic plant, and process for the preparation of tocopherols BR0013127A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19937957A DE19937957A1 (en) 1999-08-11 1999-08-11 Homogenate dioxygenase
PCT/EP2000/007807 WO2001012827A2 (en) 1999-08-11 2000-08-10 Method for production of transgenic plants with increased tocopherol content

Publications (1)

Publication Number Publication Date
BR0013127A true BR0013127A (en) 2002-04-23

Family

ID=7917993

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0013127-0A BR0013127A (en) 1999-08-11 2000-08-10 Expression cassette, recombinant vector, microorganism, transgenic plant, method for generating transgenic plants, uses of a microorganism vector, an expression cassette, or a transgenic plant, and process for the preparation of tocopherols

Country Status (7)

Country Link
EP (1) EP1200598A2 (en)
AR (1) AR029762A1 (en)
AU (1) AU6440900A (en)
BR (1) BR0013127A (en)
CA (1) CA2381316A1 (en)
DE (1) DE19937957A1 (en)
WO (1) WO2001012827A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2253220T3 (en) 1999-04-15 2006-06-01 Calgene Llc SEQUENCES OF NUCLEIC ACID OF PROTEINS INVOLVED IN THE SYNTHESIS OF TOCOFEROL.
US6872815B1 (en) 2000-10-14 2005-03-29 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
DE60140312D1 (en) 2000-08-07 2009-12-10 Monsanto Technology Llc Am methyl-d-erythriol phosphat reaktionsweg beteiligte gene
DE10046462A1 (en) * 2000-09-19 2002-05-29 Sungene Gmbh & Co Kgaa Improved procedures for vitamin E biosynthesis
US7161061B2 (en) 2001-05-09 2007-01-09 Monsanto Technology Llc Metabolite transporters
DE60230608D1 (en) 2001-05-09 2009-02-12 Monsanto Technology Llc TYRA GENES AND ITS USE
EP1427832A4 (en) 2001-08-17 2006-09-06 Monsanto Technology Llc Methyltransferase genes and uses thereof
AU2002342114B2 (en) 2001-10-25 2008-07-31 Monsanto Technology Llc Aromatic methyltransferases and uses thereof
BRPI0308740B1 (en) 2002-03-19 2018-11-21 Monsanto Technology Llc nucleic acid molecule encoding a homogenous prenyl transferase (“hpt”) and method of producing plant having increased tocopherol level seed
US7230165B2 (en) 2002-08-05 2007-06-12 Monsanto Technology Llc Tocopherol biosynthesis related genes and uses thereof
GB2595633A (en) * 2020-04-15 2021-12-08 Univ Liverpool Anti-infective agents

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA199900150A1 (en) * 1996-07-25 2000-04-24 Америкэн Сиэнамид Компани HPPD gene and inhibitors
DE19730066A1 (en) * 1997-07-14 1999-01-21 Basf Ag DNA sequence coding for a hydroxyphenylpyruvate dioxygenase and its overproduction in plants
FR2778527A1 (en) * 1998-05-18 1999-11-19 Rhone Poulenc Agrochimie Producing tomato, cereal or oilseed plants, especially maize, oilseed rape, soya or sunflower plants, from which tocopherols can be extracted and purified
CA2339519A1 (en) * 1998-08-05 2000-02-17 Sungene Gmbh & Co.Kgaa Dna sequence coding for a 1-deoxy-d-xylulose-5-phosphate synthase and the overproduction thereof in plants

Also Published As

Publication number Publication date
DE19937957A1 (en) 2001-02-15
AU6440900A (en) 2001-03-13
WO2001012827A9 (en) 2002-05-10
WO2001012827A3 (en) 2001-08-23
AR029762A1 (en) 2003-07-16
WO2001012827A2 (en) 2001-02-22
CA2381316A1 (en) 2001-02-22
EP1200598A2 (en) 2002-05-02

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