ZA856640B - New process for the disruption of bacteria cells containing recombinant polypeptides - Google Patents
New process for the disruption of bacteria cells containing recombinant polypeptidesInfo
- Publication number
- ZA856640B ZA856640B ZA856640A ZA856640A ZA856640B ZA 856640 B ZA856640 B ZA 856640B ZA 856640 A ZA856640 A ZA 856640A ZA 856640 A ZA856640 A ZA 856640A ZA 856640 B ZA856640 B ZA 856640B
- Authority
- ZA
- South Africa
- Prior art keywords
- cells containing
- disruption
- new process
- recombinant polypeptides
- containing recombinant
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
- C12N1/066—Lysis of microorganisms by physical methods
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
1. Process for isolating a polypeptide prepared by recombinant methods from bacterial cells, characterised in that bacterial cells containing a polypeptide prepared by genetic engineering, these cells being suspended in an aqueous acid at a pH of from 2.0 to 4.0, are mechanically destroyed by shear forces and subsequently the polypeptide now present in the resulting suspension is separated and further purified by known methods.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19843432196 DE3432196A1 (en) | 1984-09-01 | 1984-09-01 | NEW MECHANICAL DIGESTIVE METHOD OF BACTERIAL CELLS FOR ISOLATING RECOMBINANTLY PRODUCED PEPTIDES |
Publications (1)
Publication Number | Publication Date |
---|---|
ZA856640B true ZA856640B (en) | 1987-05-27 |
Family
ID=6244436
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ZA856640A ZA856640B (en) | 1984-09-01 | 1985-08-30 | New process for the disruption of bacteria cells containing recombinant polypeptides |
Country Status (12)
Country | Link |
---|---|
EP (1) | EP0173924B1 (en) |
JP (1) | JPH078238B2 (en) |
KR (1) | KR930002886B1 (en) |
AT (1) | ATE49231T1 (en) |
DE (2) | DE3432196A1 (en) |
DK (1) | DK165748C (en) |
ES (1) | ES8608038A1 (en) |
FI (1) | FI85979C (en) |
HU (1) | HU193806B (en) |
IE (1) | IE58521B1 (en) |
IL (1) | IL76264A (en) |
ZA (1) | ZA856640B (en) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5196323A (en) * | 1985-04-27 | 1993-03-23 | Boehringer Ingelheim International Gmbh | Process for preparing and purifying alpha-interferon |
US4675387A (en) * | 1985-07-26 | 1987-06-23 | E. I. Du Pont De Nemours And Company | Method for extracting protein with organic acid |
US4734362A (en) * | 1986-02-03 | 1988-03-29 | Cambridge Bioscience Corporation | Process for purifying recombinant proteins, and products thereof |
US5831022A (en) * | 1986-02-18 | 1998-11-03 | Hoffmann-La Roche Inc. | Purification of recombinant human IL-1α |
US4801686A (en) * | 1986-09-04 | 1989-01-31 | Immunex Corporation | Purification of recombinant interleukin-1 |
US5179199A (en) * | 1986-10-20 | 1993-01-12 | Genzyme Corporation | Protein purification |
US4912200A (en) * | 1987-05-11 | 1990-03-27 | Schering Corporation | Extraction of granulocyte macrophage colony stimulating factor from bacteria |
US4958007A (en) * | 1988-05-17 | 1990-09-18 | Schering-Plough Corp. | Extraction of human interleukin-4- from bacteria |
US6967241B2 (en) | 2002-09-06 | 2005-11-22 | Genentech, Inc. | Process for protein extraction |
JP4779537B2 (en) * | 2005-09-28 | 2011-09-28 | パナソニック株式会社 | Vacuum cleaner |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1432039A (en) * | 1972-11-10 | 1976-04-14 | Dai Nippon Sugar Mfg Co Ltd | Method of treating microorganisms |
JPS5530834A (en) * | 1978-08-25 | 1980-03-04 | Nec Corp | Method of forming ohmic contact in semiconductor pellet |
US4315852A (en) * | 1980-11-26 | 1982-02-16 | Schering Corporation | Extraction of interferon from bacteria |
JPS57118798A (en) * | 1980-11-26 | 1982-07-23 | Schering Plough Corp | Obtaining of interferon solution |
WO1983002461A1 (en) * | 1982-01-19 | 1983-07-21 | Cetus Corp | Multiclass hybrid interferons |
WO1984002129A1 (en) * | 1982-11-22 | 1984-06-07 | Takeda Chemical Industries Ltd | Human immune interferon protein and process for its preparation |
-
1984
- 1984-09-01 DE DE19843432196 patent/DE3432196A1/en not_active Withdrawn
-
1985
- 1985-08-23 EP EP85110599A patent/EP0173924B1/en not_active Expired - Lifetime
- 1985-08-23 AT AT85110599T patent/ATE49231T1/en not_active IP Right Cessation
- 1985-08-23 DE DE8585110599T patent/DE3575162D1/en not_active Expired - Fee Related
- 1985-08-29 ES ES546528A patent/ES8608038A1/en not_active Expired
- 1985-08-30 IE IE214185A patent/IE58521B1/en not_active IP Right Cessation
- 1985-08-30 FI FI853324A patent/FI85979C/en not_active IP Right Cessation
- 1985-08-30 ZA ZA856640A patent/ZA856640B/en unknown
- 1985-08-30 IL IL76264A patent/IL76264A/en not_active IP Right Cessation
- 1985-08-30 JP JP60191818A patent/JPH078238B2/en not_active Expired - Lifetime
- 1985-08-30 HU HU853309A patent/HU193806B/en not_active IP Right Cessation
- 1985-08-30 KR KR1019850006323A patent/KR930002886B1/en not_active IP Right Cessation
- 1985-08-30 DK DK396685A patent/DK165748C/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
KR930002886B1 (en) | 1993-04-12 |
DK396685A (en) | 1986-03-02 |
IE58521B1 (en) | 1993-10-06 |
JPH078238B2 (en) | 1995-02-01 |
DK165748C (en) | 1993-06-14 |
ES8608038A1 (en) | 1986-06-01 |
HUT39471A (en) | 1986-09-29 |
DK396685D0 (en) | 1985-08-30 |
IL76264A0 (en) | 1986-01-31 |
DK165748B (en) | 1993-01-11 |
DE3432196A1 (en) | 1986-03-06 |
DE3575162D1 (en) | 1990-02-08 |
FI853324A0 (en) | 1985-08-30 |
FI85979B (en) | 1992-03-13 |
ES546528A0 (en) | 1986-06-01 |
HU193806B (en) | 1987-12-28 |
IE852141L (en) | 1986-03-01 |
EP0173924B1 (en) | 1990-01-03 |
EP0173924A3 (en) | 1988-02-03 |
FI853324L (en) | 1986-03-02 |
IL76264A (en) | 1990-02-09 |
FI85979C (en) | 1992-06-25 |
ATE49231T1 (en) | 1990-01-15 |
KR860002571A (en) | 1986-04-26 |
EP0173924A2 (en) | 1986-03-12 |
JPS6170984A (en) | 1986-04-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU644359B2 (en) | Lymphokine production and purification | |
ZA856640B (en) | New process for the disruption of bacteria cells containing recombinant polypeptides | |
ZA887773B (en) | Interleukin-7 | |
ZA869487B (en) | Process for the improvement of the expression of eukaryont proteins | |
HUT38398A (en) | Process for the isolation of purified growth hormone produced by microorganisms modified by genetic engineering | |
AU2209988A (en) | Method for increasing the expression of polypeptides in recombinant cell culture | |
EP0300425A3 (en) | Method for the production of proteins in a soluble form | |
IE881779L (en) | Dna fragments, containing a lactic acid bacterium-specific¹regulator region for the expression of genes coding for¹normally heterologous proteins, recombinant plasmids based¹on such dna fragments, lactic acid bacteria which contain¹these recombinant plasmids and the heterologous proteins and¹products prepared with the aid of the above microorganisms | |
NZ216723A (en) | Production of insecticidal protein by genetic engineering methods | |
EP0201882A3 (en) | Fish prolactin polypeptide and derivatives thereof | |
CS78688A1 (en) | Method of foreign proteins' secretory production by means of transformed yeast cells cultivation |