DK396685A - PROCEDURE FOR MECHANICAL SELECTION OF BACTERIA CELLS FOR ISOLATING PEPTIDES - Google Patents

PROCEDURE FOR MECHANICAL SELECTION OF BACTERIA CELLS FOR ISOLATING PEPTIDES Download PDF

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Publication number
DK396685A
DK396685A DK396685A DK396685A DK396685A DK 396685 A DK396685 A DK 396685A DK 396685 A DK396685 A DK 396685A DK 396685 A DK396685 A DK 396685A DK 396685 A DK396685 A DK 396685A
Authority
DK
Denmark
Prior art keywords
procedure
bacteria cells
mechanical selection
isolating peptides
isolating
Prior art date
Application number
DK396685A
Other languages
Danish (da)
Other versions
DK165748C (en
DK396685D0 (en
DK165748B (en
Inventor
Guenter Adolf
Gerhard Bodo
Wolfgang Gibley
Kurt Konopitzky
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Publication of DK396685D0 publication Critical patent/DK396685D0/en
Publication of DK396685A publication Critical patent/DK396685A/en
Publication of DK165748B publication Critical patent/DK165748B/en
Application granted granted Critical
Publication of DK165748C publication Critical patent/DK165748C/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

1. Process for isolating a polypeptide prepared by recombinant methods from bacterial cells, characterised in that bacterial cells containing a polypeptide prepared by genetic engineering, these cells being suspended in an aqueous acid at a pH of from 2.0 to 4.0, are mechanically destroyed by shear forces and subsequently the polypeptide now present in the resulting suspension is separated and further purified by known methods.
DK396685A 1984-09-01 1985-08-30 PROCEDURE FOR INSULATING A RECOMBINANT PREPARED POLYPEPTIDE FROM BACTERY CELLS DK165748C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3432196 1984-09-01
DE19843432196 DE3432196A1 (en) 1984-09-01 1984-09-01 NEW MECHANICAL DIGESTIVE METHOD OF BACTERIAL CELLS FOR ISOLATING RECOMBINANTLY PRODUCED PEPTIDES

Publications (4)

Publication Number Publication Date
DK396685D0 DK396685D0 (en) 1985-08-30
DK396685A true DK396685A (en) 1986-03-02
DK165748B DK165748B (en) 1993-01-11
DK165748C DK165748C (en) 1993-06-14

Family

ID=6244436

Family Applications (1)

Application Number Title Priority Date Filing Date
DK396685A DK165748C (en) 1984-09-01 1985-08-30 PROCEDURE FOR INSULATING A RECOMBINANT PREPARED POLYPEPTIDE FROM BACTERY CELLS

Country Status (12)

Country Link
EP (1) EP0173924B1 (en)
JP (1) JPH078238B2 (en)
KR (1) KR930002886B1 (en)
AT (1) ATE49231T1 (en)
DE (2) DE3432196A1 (en)
DK (1) DK165748C (en)
ES (1) ES8608038A1 (en)
FI (1) FI85979C (en)
HU (1) HU193806B (en)
IE (1) IE58521B1 (en)
IL (1) IL76264A (en)
ZA (1) ZA856640B (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5196323A (en) * 1985-04-27 1993-03-23 Boehringer Ingelheim International Gmbh Process for preparing and purifying alpha-interferon
US4675387A (en) * 1985-07-26 1987-06-23 E. I. Du Pont De Nemours And Company Method for extracting protein with organic acid
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
US5831022A (en) * 1986-02-18 1998-11-03 Hoffmann-La Roche Inc. Purification of recombinant human IL-1α
US4801686A (en) * 1986-09-04 1989-01-31 Immunex Corporation Purification of recombinant interleukin-1
US5179199A (en) * 1986-10-20 1993-01-12 Genzyme Corporation Protein purification
US4912200A (en) * 1987-05-11 1990-03-27 Schering Corporation Extraction of granulocyte macrophage colony stimulating factor from bacteria
US4958007A (en) * 1988-05-17 1990-09-18 Schering-Plough Corp. Extraction of human interleukin-4- from bacteria
US6967241B2 (en) 2002-09-06 2005-11-22 Genentech, Inc. Process for protein extraction
JP4779537B2 (en) * 2005-09-28 2011-09-28 パナソニック株式会社 Vacuum cleaner

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1432039A (en) * 1972-11-10 1976-04-14 Dai Nippon Sugar Mfg Co Ltd Method of treating microorganisms
JPS5530834A (en) * 1978-08-25 1980-03-04 Nec Corp Method of forming ohmic contact in semiconductor pellet
US4315852A (en) * 1980-11-26 1982-02-16 Schering Corporation Extraction of interferon from bacteria
JPS57118798A (en) * 1980-11-26 1982-07-23 Schering Plough Corp Obtaining of interferon solution
WO1983002461A1 (en) * 1982-01-19 1983-07-21 Cetus Corp Multiclass hybrid interferons
WO1984002129A1 (en) * 1982-11-22 1984-06-07 Takeda Chemical Industries Ltd Human immune interferon protein and process for its preparation

Also Published As

Publication number Publication date
KR930002886B1 (en) 1993-04-12
IE58521B1 (en) 1993-10-06
JPH078238B2 (en) 1995-02-01
DK165748C (en) 1993-06-14
ES8608038A1 (en) 1986-06-01
HUT39471A (en) 1986-09-29
DK396685D0 (en) 1985-08-30
IL76264A0 (en) 1986-01-31
DK165748B (en) 1993-01-11
DE3432196A1 (en) 1986-03-06
DE3575162D1 (en) 1990-02-08
FI853324A0 (en) 1985-08-30
FI85979B (en) 1992-03-13
ES546528A0 (en) 1986-06-01
HU193806B (en) 1987-12-28
IE852141L (en) 1986-03-01
EP0173924B1 (en) 1990-01-03
EP0173924A3 (en) 1988-02-03
FI853324L (en) 1986-03-02
ZA856640B (en) 1987-05-27
IL76264A (en) 1990-02-09
FI85979C (en) 1992-06-25
ATE49231T1 (en) 1990-01-15
KR860002571A (en) 1986-04-26
EP0173924A2 (en) 1986-03-12
JPS6170984A (en) 1986-04-11

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