ZA200506553B - Purification process for bacterial cytolysin - Google Patents

Info

Publication number

ZA200506553B

ZA200506553B ZA200506553A ZA200506553A ZA200506553B ZA 200506553 B ZA200506553 B ZA 200506553B ZA 200506553 A ZA200506553 A ZA 200506553A ZA 200506553 A ZA200506553 A ZA 200506553A ZA 200506553 B ZA200506553 B ZA 200506553B

Authority

ZA

South Africa

Prior art keywords

pneumolysin

bacterial

cytolysin

detergent

bacterial cytolysin

Prior art date

2003-03-13

Links

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• 102000004503 Perforin Human genes 0.000 title claims description 84
• 230000001580 bacterial effect Effects 0.000 title claims description 44
• 238000000746 purification Methods 0.000 title claims description 19
• 101710183389 Pneumolysin Proteins 0.000 claims description 208
• 238000000034 method Methods 0.000 claims description 125
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• 229960005486 vaccine Drugs 0.000 claims description 67
• 235000002639 sodium chloride Nutrition 0.000 claims description 57
• 102000004169 proteins and genes Human genes 0.000 claims description 52
• 108090000623 proteins and genes Proteins 0.000 claims description 52
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• 150000003839 salts Chemical class 0.000 claims description 48
• PVGATNRYUYNBHO-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 4-(2,5-dioxopyrrol-1-yl)butanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCCN1C(=O)C=CC1=O PVGATNRYUYNBHO-UHFFFAOYSA-N 0.000 claims description 47
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• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 17
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• PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims description 10
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• 125000003118 aryl group Chemical group 0.000 claims description 5
• NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 4
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