WO2023155853A1 - 3-甲基-2-甲氨基戊酸在促进植物生长上的应用 - Google Patents

3-甲基-2-甲氨基戊酸在促进植物生长上的应用 Download PDF

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WO2023155853A1
WO2023155853A1 PCT/CN2023/076582 CN2023076582W WO2023155853A1 WO 2023155853 A1 WO2023155853 A1 WO 2023155853A1 CN 2023076582 W CN2023076582 W CN 2023076582W WO 2023155853 A1 WO2023155853 A1 WO 2023155853A1
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plant
plant growth
acid
growth
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陈世国
王赫
王良省
房婉萍
郭爱平
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南京天秾生物技术有限公司
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

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  • the invention belongs to the field of agricultural biological pesticides and relates to the application of 3-methyl-2-aminovaleric acid in promoting plant growth.
  • Plant growth regulators refer to artificially synthesized or extracted compounds with natural plant hormone physiological activity, which can be used to regulate or control certain processes of plant growth and development, such as cell division and elongation, tissue and organ differentiation, seed dormancy and germination, Flowering and fruiting, maturity and aging, etc., to promote or inhibit seed germination, plant growth, fruit ripening, flower preservation or fruit thinning, improve plant immunity, help plants resist adverse environments, reduce diseases, and increase crop yields , Improve crop quality and other purposes. Because of its significant and efficient regulating effect, it has been widely used in various aspects such as grain, fruit trees, forest trees, vegetables, and flowers. At present, my country has become one of the countries where plant growth regulators are widely used in the world.
  • Plant growth regulators have played a huge role in regulating crop growth, solved many problems that cannot be solved by traditional agronomic means, and made important contributions to my country's agricultural production and development.
  • the food safety problems caused by the blind and excessive use of plant growth regulators still exist widely, and the international standards for the residue limit of plant growth regulators are becoming more and more stringent. Therefore, it is very important to develop low-toxicity, high-efficiency, and environment-friendly natural plant growth regulators to ensure green and safe agricultural production.
  • 3-Methyl-2-methylaminovaleric acid the molecular formula is C 7 H 15 NO 2 , the molecular weight is 145 g/mol, and it is a colorless crystal. It is a structural component of a variety of natural products. Fusarin A (Plattner & Nager, 1948), indole lactams produced by Streptomyces and Nocardiopsis (Irie et al., 1990; Sun et al., 1991) and cyanobacterial cyclic The structures of peptides (Luo et al., 2014) all contain 3-methyl-2-methylaminovaleric acid. In 1996, Li et al.
  • the object of the present invention is to address the above-mentioned deficiencies of the prior art, and to provide the application of 3-methyl-2-aminovaleric acid (I) as a growth regulator to promote plant growth.
  • the application of 3-methyl-2-methylaminovaleric acid to promote the occurrence and growth of root system of plant seedlings through hydroponic treatment As a preference of the present invention, the application of 3-methyl-2-methylaminovaleric acid to promote the occurrence and growth of root system of plant seedlings through hydroponic treatment.
  • the plant is preferably cucumber.
  • the plant is preferably cucumber.
  • 3-methyl-2-methylaminovaleric acid did not involve reports in the field of plant growth regulators.
  • my country is one of the major countries in the application of plant growth regulators.
  • 3-methyl-2-methylaminovaleric acid performed well in related experiments on promoting plant growth, and could significantly promote plant growth.
  • a method for improving plant growth characterized in that 10-1000nM of 3-methyl-2-aminovaleric acid is applied to target plants.
  • the natural product 3-methyl-2-aminovaleric acid is used to promote the rooting of cucumber seedlings. It can significantly promote Cucumber seedling growth; especially when the concentration was 100nM, compared with the blank control, the root length, lateral root number, root fresh weight and whole plant fresh weight of cucumber increased by 205%, 69%, 167% and 54% respectively.
  • 3-methyl-2-methylaminovaleric acid is used to promote the growth of cucumber seedlings.
  • concentration 100nM
  • the root irrigation method is used to treat cucumber soil-cultured seedlings, which can significantly promote the growth of cucumber seedlings. Growth; compared with the blank control group, plant height, leaf area of the first true leaf, leaf area of the second true leaf, The fresh weight of the whole plant, the fresh weight of the aboveground part, the fresh weight of the underground part and the root length increased by 22%, 62%, 94%, 54%, 40%, 132% and 48% respectively.
  • 3-Methyl-2-methylaminovaleric acid is a natural product with a simple structure and easy industrial production.
  • the present invention confirms that 3-methyl-2-methylaminovaleric acid can promote plant growth, and 3-methyl-2-methylaminovaleric acid can promote the growth and development of plants by doing hydroponics and root watering treatment, and can significantly improve the growth and development of plants.
  • 3-methyl-2-aminovaleric acid is a natural product with low dosage, high environmental and biological safety, and has the potential to be developed into a natural Potential as a plant growth regulator.
  • the inventor conducted research on the biological activity and crop safety of 3-methyl-2-methylaminovaleric acid, and found that the substance has a very unique role in regulating crop growth, and has the advantages of environmental friendliness, high activity, and safe use. , has the potential to be developed as a biopesticide.
  • the substantive features of the present invention can be realized from the following embodiments and examples, but these should not be regarded as any limitation to the invention.

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  • Life Sciences & Earth Sciences (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Agronomy & Crop Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

提供一种3-甲基-2-甲氨基戊酸在促进植物生长上的应用。将3-甲基-2-甲氨基戊酸作为天然活性物质开发为植物生长调节剂可用于促进植物植株的生长，能显著提高植物的株高、根长、侧根数、真叶面积、植株鲜重等。3-甲基-2-甲氨基戊酸具有安全、环保、高效的特点。

Description

3-甲基-2-甲氨基戊酸在促进植物生长上的应用 技术领域
本发明属于农业生物农药领域，涉及3-甲基-2-甲氨基戊酸在促进植物生长上的应用。
背景技术
植物生长调节剂是指人工合成或提取的具有天然植物激素生理活性的化合物，可用于调节或控制植物生长发育的某些过程，如细胞***与伸长、组织与器官分化、种子休眠与萌发、开花与结实、成熟与衰老等，以达到促进或抑制种子萌发、植物生长、果实成熟、保花保果或疏花疏果、提高植物免疫力、帮助植物抵抗不良环境、减轻病害、增加作物产量、改善作物品质等目的。因其显著、高效的调节效应，已被广泛地应用于粮食、果树、林木、蔬菜、花卉等各个方面。目前我国已成为世界上植物生长调节剂应用最广泛的国家之一。
植物生长调节剂在调控作物生长方面发挥了巨大的作用，解决了诸多传统农艺手段无法解决的问题，为我国农业生产和发展做出了重要的贡献，已经成为农业增产、提质和增效的主要措施之一。但盲目、过量使用植物生长调节剂所引起的食品安全问题仍然广泛存在，国际上对于植物生长调节剂残留限量标准也越来越严格。因此，开发低毒、高效、环境友好型的天然植物生长调节剂对于保证农业绿色安全生产十分重要。
3-甲基-2-甲氨基戊酸，分子式为C7H15NO2，分子量为145克/摩尔，为无色晶体。它是多种天然产物的结构组成部分。镰孢菌素A(Plattner & Nager,1948)、链霉菌和拟诺卡菌(Nocardiopsis)产生的吲哚内酰胺类化合物(Irie et al.,1990；Sun et al.,1991)和蓝藻环状多肽(Luo et al.,2014)的结构中均含有3-甲基-2-甲氨基戊酸。1996年，Li等人成功从菜豆(Phaseolus vulgaris)种子中分离得到了3-甲基-2-甲氨基戊酸(Li et al.,1996)。最近，我们从真菌链格孢菌中成功分离、纯化得到3-甲基-2-甲氨基戊酸。截至目前，还没有涉及该化合物促进植物生长活性方面的研究、报道和专利。
发明内容
本发明的目的是针对现有技术的上述不足，提供3-甲基-2-甲氨基戊酸(I)作为生长调节剂促进植物生长的应用。
本发明的目的可通过以下技术方案实现：
3-甲基-2-甲氨基戊酸在促进植物幼苗生长的应用。
作为本发明的一种优选,3-甲基-2-甲氨基戊酸通过水培处理促进植物幼苗根系的发生和生长的应用。
作为本发明的一种优选,3-甲基-2-甲氨基戊酸通过灌根处理促进植物幼苗生长的应用。
作为本发明的一种优选,所述的植物优选黄瓜。
3-甲基-2-甲氨基戊酸在制备植物生长调节剂中的应用。
作为本发明的一种优选,所述的植物优选黄瓜。
3-甲基-2-甲氨基戊酸的已有的相关研究并未涉及植物生长调节剂领域的报道。我国是植物生长调节剂的应用大国之一，随着公众对食品安全和健康的日益重视，开发低毒、高效、环境友好型的植物生长调节剂显得尤为迫切。因此，发展天然植物生长调节剂，并推动其产业化，对于保障食品安全、提高农产品竞争力具有重要的意义。3-甲基-2-甲氨基戊酸在促进植物生长的相关实验中表现良好，能够显著促进植物生长。
一种提高植物植株生长的方法，其特征在于向目标植物施加10-1000nM的3-甲基-2-甲氨基戊酸。
作为本发明的一种优选，天然产物3-甲基-2-甲氨基戊酸用于促进黄瓜幼苗生根的方法，其在浓度10-1000nM范围，采用水培的方式处理黄瓜幼苗，能够显著促进黄瓜幼苗生长；尤其是在浓度为100nM时，与空白对照相比黄瓜的根长、侧根数、根鲜重和整株鲜重分别提高了205％、69％、167％和54％。
作为本发明的一种优选，3-甲基-2-甲氨基戊酸用于促进黄瓜幼苗生长的方法，其在浓度为100nM时，采用灌根方式处理黄瓜土培幼苗，能够显著促进黄瓜幼苗生长；与空白对照组相比株高、第一片真叶叶面积、第二片真叶叶面积、 整株鲜重、地上部分鲜重、地下部分鲜重和根长分别提高了22％、62％、94％、54％、40％、132％和48％。
技术先进性和有益效果
本发明的主要优点和积极效果如下：
3-甲基-2-甲氨基戊酸是一种天然产物，结构简单，易于工业化生产。本发明确认了3-甲基-2-甲氨基戊酸能促进植物生长，3-甲基-2-甲氨基戊酸做水培和根部浇灌处理可以促进植物的生长发育，能显著提高植物的株高、根长、侧根数、真叶面积、植株鲜重等，3-甲基-2-甲氨基戊酸为天然产物，用量低，具有很高的环境和生物安全性，具有开发成天然植物生长调节剂的潜力。
具体实施方式
发明人对3-甲基-2-甲氨基戊酸进行了生物活性及作物安全性研究，发现该物质在调控作物生长方面具有非常独特的作用，并且具有环境友好、活性高、使用安全等优点，具备开发为生物农药的潜力。本发明的实质性特点可以从下述的实施方案和实施例中得以体现，但这些不应视为是对发明的任何限制。
实施例1：3-甲基-2-甲氨基戊酸对黄瓜幼苗生根的影响
为了研究3-甲基-2-甲氨基戊酸对植物根系生长的促进作用，一个研究侧根发生的模式***—去胚根的黄瓜幼苗体系被选用。在培养盒底部平铺四层湿润的纱布，挑选形态饱满、大小均一的黄瓜种子(品种为“初夏秋冠”，山东宁阳县鲁明种子有限公司)，75％酒精消毒，用蒸馏水冲洗干净后均匀的撒在纱布上，再用湿润的四层纱布盖上避光。在25℃培养室中避光24小时后，取出萌芽的黄瓜种子，埋入土壤下1厘米(cm)处。3天后，取长势一致，两片子叶完全舒展开的幼苗若干，用蒸馏水将根部冲洗干净，沿下胚轴处剪掉胚根，用海绵条固定植株，放置于培养盒中。每盒分别添加浓度为0、10、100和1000nM的3-甲基-2-甲氨基戊酸水溶液400毫升(mL)。将水培的植株放于25℃培养室，光照培养6天后，取出测量和记录根长、侧根数、根鲜重和整株鲜重。结果见表1。
表1 不同浓度3-甲基-2-甲氨基戊酸对黄瓜幼苗生根的影响

由表1可知，10-1000nM的3-甲基-2-甲氨基戊酸均能够促进黄瓜幼苗的生根。在浓度为10、100和1000nM时，与空白对照相比黄瓜的根长分别提高了102％、205％和103％；侧根数数分别提高了53％、69％和54％；根鲜重分别提高了67％、167％、33％；整株鲜重分别提高了39％、54％、22％。其中100nM 3-甲基-2-甲氨基戊酸处理的效果最佳。以上结果说明3-甲基-2-甲氨基戊酸能够显著促进黄瓜幼苗的根系的发生和生长。
实施例2：3-甲基-2-甲氨基戊酸对黄瓜幼苗生长的影响
在培养盒底部平铺四层湿润的纱布，挑选形态饱满、大小均一的黄瓜种子(品种为“初夏秋冠”，山东宁阳县鲁明种子有限公司)，75％酒精消毒，用蒸馏水冲洗干净后均匀的撒在纱布上，再用湿润的四层纱布盖上避光。在25℃培养室中避光24小时后，取出萌芽的黄瓜种子，埋入装满湿润(饱和水分)土壤的塑料盆(直径12cm)中，深度2-3cm，每盆5粒，放置在25℃培养室(光强200μmol m-2s-1)培养。3天后，保留1株子叶完全展开的健壮幼苗，去掉其他4株幼苗。然后，在每盆中沿幼苗茎的基部缓慢浇灌浓度为0(空白对照)和100nM的3-甲基-2-甲氨基戊酸50mL，在25℃培养室继续光照培养。在处理后第6和12天，观察并记录各处理黄瓜幼苗的株高和真叶生长情况；在第18天时，测量各处理黄瓜幼苗的株高、第一片真叶面积、第二片真叶面积、第三片真叶面积、整株鲜重、地上部分鲜重、地下部分鲜重和根长。结果见表2。
表2 3-甲基-2-甲氨基戊酸对黄瓜植株幼苗生长的影响

表2结果表明，与空白对照相比，3-甲基-2-甲氨基戊酸能够显著促进黄瓜幼苗生长。例如在第12天，空白对照组还未长出第二片真叶，而经过100nM的3-甲基-2-甲氨基戊酸处理的黄瓜幼苗已分别长出了叶面积为9.74cm2的第二片真叶；在第18天，空白对照组还未长出第三片真叶，而此时经100nM的3-甲基-2-甲氨基戊酸处理的黄瓜幼苗已经长出了叶面积为4.16cm2的第三片真叶。在第18天时，与空白对照相比，经100nM的3-甲基-2-甲氨基戊酸处理的黄瓜幼苗的株高、第一片真叶叶面积、第二片真叶叶面积、整株鲜重、地上部分鲜重、地下部分鲜重和根长分别提高了22％、62％、94％、54％、40％、132％和48％。这说明3-甲基-2-甲氨基戊酸能够显著促进幼苗的发育，促进根、茎、叶的发育和快速生长。

Claims (4)

  1. 3-甲基-2-甲氨基戊酸在制备植物生长调节剂中的应用。
  2. 3-甲基-2-甲氨基戊酸在促进植物生长中的应用。
  3. 根据权利要求1或2所述的应用，其特征在于，所述的植物，选自蔬菜。所述的蔬菜优选黄瓜。
  4. 一种提高植物植株生长的方法，其特征在于向目标植物施加10-1000 nM的3-甲基-2-甲氨基戊酸。
PCT/CN2023/076582 2022-02-18 2023-02-16 3-甲基-2-甲氨基戊酸在促进植物生长上的应用 WO2023155853A1 (zh)

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