WO2021223385A1 - Adjustable centrifugal rotary disc and use thereof - Google Patents
Adjustable centrifugal rotary disc and use thereof Download PDFInfo
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- WO2021223385A1 WO2021223385A1 PCT/CN2020/126039 CN2020126039W WO2021223385A1 WO 2021223385 A1 WO2021223385 A1 WO 2021223385A1 CN 2020126039 W CN2020126039 W CN 2020126039W WO 2021223385 A1 WO2021223385 A1 WO 2021223385A1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
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- This article relates to a centrifugal turntable, especially a centrifugal turntable with adjustable centrifugal radius.
- centrifugal technology and centrifuges have been widely used. Different components in the liquid have different sedimentation speeds, and they can be separated from each other under the action of centrifugal force.
- low-speed centrifugation can be used to settle particles or cells in a liquid
- a high-speed centrifuge can be used to settle subcellular structures from a liquid
- an ultra-high-speed centrifuge can be used to separate biological macromolecules with different molecular weights.
- microfluidic chip or microfluidic testing card technology has gradually been applied.
- Microfluidic chips have the advantages of small sample volume requirements, low testing reagent consumption, and suitable for high-throughput testing.
- Some microfluidic chips rely on capillary action for detection, while more microfluidic chips need to drive the directional or reciprocating flow of various liquids in the chip under the action of centrifugal force.
- Figure 1 shows an example of a microfluidic chip for blood group detection. In the process of designing a microfluidic chip or the use of a microfluidic chip, it is often necessary to study how much centrifugal force can be used to accurately control the flow of liquid in the microfluidic chip in various chambers and channels.
- the current research methods are mostly through experiments, adjusting the speed of the centrifuge several times to find a suitable centrifugal speed; or changing the centrifugal turntable many times to find a suitable centrifugal radius. This method is obviously time-consuming, laborious and costly.
- this article provides an adjustable centrifugal turntable, which includes a centrifugal turntable body and a plurality of positioning modules arranged on the centrifugal turntable body, the positioning module includes a plurality of positioning members sequentially arranged in the direction of the centrifugal radius The positioning member determines the centrifugal radius of the centrifugal member on the centrifugal turntable during centrifugation.
- the centrifuged part is a test card.
- the multiple positioning modules are symmetrically arranged on the centrifugal turntable.
- the positioning members are positioning holes arranged in pairs.
- the positioning module further includes a card slot for accommodating the detection card; the card slot is provided with a card that cooperates with the positioning member to fix the card slot on the centrifugal turntable. Slot positioning structure.
- the slot positioning structure is a pair of slot positioning holes or slot positioning pins.
- the centrifugal turntable further includes a plurality of fixing pins for fixing the slot with the slot positioning hole on the centrifugal turntable.
- the centrifugal turntable further includes a plurality of connecting handles with two positioning pins for fixing the slot with the positioning hole of the slot on the centrifugal turntable.
- this article provides the application of the above-mentioned adjustable centrifugal turntable for centrifuging the microfluidic chip.
- this article provides a centrifuge including the above-mentioned adjustable centrifugal turntable.
- the adjustable centrifugal turntable provided in this article can easily verify the influence of different centrifugal radii on the results of centrifugation experiments while avoiding batch-to-batch differences caused by changes in the centrifugal environment.
- Figure 1 is a schematic diagram of the shape of a microfluidic chip for blood group detection.
- Figure 2 is a schematic diagram of an embodiment of the adjustable centrifugal turntable of the present invention.
- Fig. 3 is a schematic diagram of another embodiment of the adjustable centrifugal turntable of the present invention.
- Fig. 4 is a partial enlarged view of the adjustable centrifugal turntable in Fig. 3, showing the structure of the card slot.
- Fig. 5 is a schematic diagram of using a fixing pin to fix the card slot.
- Fig. 6 is a schematic diagram of using a connecting handle with a fixing pin to fix the card slot.
- Figure 7 shows the matching relationship between the card slot with the protrusion and the radial slot provided on the centrifugal turntable.
- Fig. 8 is a schematic diagram of using the adjustable centrifugal turntable of the present invention to position different slots at different centrifugal radii.
- Figure 9 is a partial enlarged view of the centrifugal turntable with the test card, showing the test card placed in the card slot.
- plurality includes two and more than two.
- the "adjustable type" used means that when the test card is centrifuged on the centrifugal turntable, the centrifugal radius can be adjusted before centrifugation.
- the centrifugal radii can be adjusted respectively before centrifugation, so that the centrifugal radii of at least two detection cards are different.
- the centrifugal radius of these test cards can also be adjusted to make them all the same.
- Test card refers to a card used for various testing purposes, including various miniature blood type cards and microfluidic chips, for example. Their common shape is a thin cube. Of course, detection cards of other shapes or uses can also be included, as long as they can be conveniently fixed on the centrifugal turntable of the present invention or in the card slot of the centrifugal turntable.
- the "symmetrical arrangement" used means that multiple detection card positioning modules are symmetrical with respect to the centrifugal axis.
- the "positioning hole” mentioned in this article is used to detect the positioning of the card or the card slot.
- the positioning slot can also be used to replace the positioning hole, and other structures to be connected with it can be adapted to be modified at the same time.
- this article does not additionally describe the embodiments using positioning grooves, but it is obvious that they should also be included in the scope of the present invention.
- the adjustable centrifugal turntable of the present invention is shown in FIG. 2. It includes a centrifugal turntable body 1, a mounting hole 2 provided on it, and 6 detection card positioning modules 3.
- the centrifugal turntable body 1 can be made of any suitable material, such as metal, glass, plastic and so on.
- the mounting hole 2 is used for fixing the centrifugal turntable body 1 and the rotating shaft of the centrifuge.
- Each detection card positioning module 3 includes a plurality of pairs of positioning holes 4.
- the pair of positioning holes 4 are arranged at intervals along the radius of the centrifugal turntable body 1. The distance of the interval can be determined according to the requirements of the experiment. In most cases, equidistant settings can be considered.
- the line between each pair of positioning holes 4 is usually perpendicular to the radius of the centrifugal turntable.
- the detection card will have different centrifugal radii during centrifugation.
- one way is to provide holes corresponding to the pair of positioning holes 4 on the detection card and fix them by fixing pins.
- Another way is to provide a card slot for accommodating the test card on the centrifugal turntable body 1, and then fix the test card indirectly by fixing the card slot (see description below).
- the adjustable centrifugal turntable of the present invention is shown in FIG. 3. It includes a centrifugal turntable body 1, a mounting hole 2 provided on it, and 6 detection card positioning modules 3'.
- Each detection card positioning module 3' includes a card slot 5 and a plurality of pairs of positioning holes 4.
- the card slot 5 is provided with a card slot hole 6 corresponding to the positioning hole 4 (see FIGS. 4 and 5).
- the fixing pin 7 can be used to fix the slot 5 to the positioning holes 4 with different centrifugal radii.
- a separate fixing pin 7 may not be used.
- two positioning pins may be directly arranged under the slot 5, which are suitable for cooperating with the positioning hole 4 to fix the slot 5.
- a connecting handle 8 with a positioning pin structure as shown in FIG. 6 can also be used to fix the card slot 5.
- the positioning hole 4 the fixing pin 7 and the slot hole 6 or the positioning pin on the slot 5 may be provided with threads, and the connection mode is changed from a plug pin to a threaded connection.
- the fixing pin 7 combined with it may also be in the form of a screw.
- a radial slot 9 can be provided between the two rows of positioning holes 4 on the centrifugal turntable body 1, and a protrusion 10 can be provided under the slot 5 (see Figure 7) .
- the width of the radial groove 9 matches the width of the protrusion 10.
- the arrangement of the radial groove 9 can also be used to adjust the overall weight of the centrifugal turntable.
- the corresponding centrifugal radius value can be marked on the turntable body 1 beside the positioning hole 4.
- FIG. 9 is a partial enlarged view of the centrifugal turntable equipped with the detection card 11, showing the detection card 11 placed in the card slot 5.
- a suitable centrifugal radius can be determined through one or a few centrifugal experiments.
- the centrifugal force applied to the test card during centrifugation is related to the centrifugal radius and the speed of the centrifuge, so in the case of a fixed centrifugal radius, a suitable centrifugal speed can be obtained by simple conversion.
- the multiple test cards fixed on the adjustable centrifugal turntable of the present invention are under the same environmental conditions, such as temperature, humidity, light, etc., which eliminates the difference in the environment where the test cards are located between multiple centrifugation operations .
- this difference is unavoidable in the traditional centrifugal radius selection method (change the speed or replace the turntable).
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Abstract
An adjustable centrifugal rotary disc, comprising a centrifugal rotary disc body (1) and a plurality of positioning modules (3) provided on the centrifugal rotary disc body; the positioning modules comprise a plurality of positioning members provided in sequence in the direction of the centrifugal radius, the positioning members being used for determining a centrifugal radius of a member subjected to a centrifugal force during centrifugation. The adjustable centrifugal rotary disc can conveniently verify the influence of different centrifugal radii on the centrifugal experiment results, and can also avoid batch-to-batch variations caused by changes in centrifugal environment.
Description
本申请要求于2020年5月7日提交至中国专利局、申请号为202010376422.4、发明名称为“可调节式离心转盘及其应用”的中国专利申请的优先权。This application claims the priority of the Chinese patent application filed to the Chinese Patent Office on May 7, 2020 with the application number 202010376422.4 and the invention title "Adjustable Centrifugal Rotary Disk and Its Application".
本文涉及一种离心转盘，尤其是离心半径可调节的离心转盘。This article relates to a centrifugal turntable, especially a centrifugal turntable with adjustable centrifugal radius.
在生物和医学领域，离心技术和离心机已被广泛应用。液体中不同成分的沉降速度不同，在离心力作用下它们可以彼此分开。例如，使用低速离心就可以将液体中的颗粒物或细胞沉降下来，高速离心机可用于从液体中沉降亚细胞结构，而超高速离心机可用于分离分子量有差异的生物大分子。In the fields of biology and medicine, centrifugal technology and centrifuges have been widely used. Different components in the liquid have different sedimentation speeds, and they can be separated from each other under the action of centrifugal force. For example, low-speed centrifugation can be used to settle particles or cells in a liquid, a high-speed centrifuge can be used to settle subcellular structures from a liquid, and an ultra-high-speed centrifuge can be used to separate biological macromolecules with different molecular weights.
近些年来，尤其是在生物或临床检测中，微流控芯片或微流控检测卡技术逐渐得到应用。微流控芯片具有样本量需求小、检测试剂消耗少、适合于高通量检测等优点。一些微流控芯片依赖毛细作用进行检测，而更多的微流控芯片需要在离心力作用下驱动芯片内各种液体的定向或往复流动。图1中显示了一个用于血型检测的微流控芯片的实例。在设计微流控芯片或微流控芯片使用过程中，经常需要研究使用多大的离心力才可以精确控制微流控芯片内液体在各个腔室和通道内的流动。目前的研究方法大多是通过实验，多次调整离心机的速度来寻找合适的离心速度；或者通过多次更换离心转盘来寻找合适的离心半径。这种方式显然是费时费力和高成本的。In recent years, especially in biological or clinical testing, microfluidic chip or microfluidic testing card technology has gradually been applied. Microfluidic chips have the advantages of small sample volume requirements, low testing reagent consumption, and suitable for high-throughput testing. Some microfluidic chips rely on capillary action for detection, while more microfluidic chips need to drive the directional or reciprocating flow of various liquids in the chip under the action of centrifugal force. Figure 1 shows an example of a microfluidic chip for blood group detection. In the process of designing a microfluidic chip or the use of a microfluidic chip, it is often necessary to study how much centrifugal force can be used to accurately control the flow of liquid in the microfluidic chip in various chambers and channels. The current research methods are mostly through experiments, adjusting the speed of the centrifuge several times to find a suitable centrifugal speed; or changing the centrifugal turntable many times to find a suitable centrifugal radius. This method is obviously time-consuming, laborious and costly.
发明内容Summary of the invention
一方面，本文提供了一种可调节式离心转盘，其包括离心转盘本体和设置在所述离心转盘本体上的多个定位模块，所述定位模块包括在离心半径方向依次设置的多个定位件，所述定位件确定离心时被离心件在所述离心转盘上的离心半径。In one aspect, this article provides an adjustable centrifugal turntable, which includes a centrifugal turntable body and a plurality of positioning modules arranged on the centrifugal turntable body, the positioning module includes a plurality of positioning members sequentially arranged in the direction of the centrifugal radius The positioning member determines the centrifugal radius of the centrifugal member on the centrifugal turntable during centrifugation.
在一些实施方案中，所述被离心件为检测卡。In some embodiments, the centrifuged part is a test card.
在一些实施方案中，所述多个定位模块在所述离心转盘上对称设置。In some embodiments, the multiple positioning modules are symmetrically arranged on the centrifugal turntable.
在一些实施方案中，所述定位件为成对设置的定位孔。In some embodiments, the positioning members are positioning holes arranged in pairs.
在一些实施方案中，所述定位模块还包括用于容纳所述检测卡的卡槽；所述卡槽上设置有与所述定位件配合以在所述离心转盘上固定所述卡槽的卡槽定位结构。In some embodiments, the positioning module further includes a card slot for accommodating the detection card; the card slot is provided with a card that cooperates with the positioning member to fix the card slot on the centrifugal turntable. Slot positioning structure.
在一些实施方案中，所述卡槽定位结构为成对设置的卡槽定位孔或卡槽定位销。In some embodiments, the slot positioning structure is a pair of slot positioning holes or slot positioning pins.
在一些实施方案中，所述离心转盘还包括用于将带有所述卡槽定位孔的所述卡槽固定在所述离心转盘上的多个固定销。In some embodiments, the centrifugal turntable further includes a plurality of fixing pins for fixing the slot with the slot positioning hole on the centrifugal turntable.
在一些实施方案中，所述离心转盘还包括用于将带有所述卡槽定位孔的所述卡槽固定在所述离心转盘上的多个带有两定位销的连接把手。In some embodiments, the centrifugal turntable further includes a plurality of connecting handles with two positioning pins for fixing the slot with the positioning hole of the slot on the centrifugal turntable.
另一方面，本文提供了上述可调节式离心转盘用于对微流控芯片进行离心的应用。On the other hand, this article provides the application of the above-mentioned adjustable centrifugal turntable for centrifuging the microfluidic chip.
另一方面，本文提供了包括上述可调节式离心转盘的离心机。In another aspect, this article provides a centrifuge including the above-mentioned adjustable centrifugal turntable.
本文提供的可调节式离心转盘，可方便地验证不同离心半径对离心实验结果的影响，同时避免离心环境变化造成的批间差异。The adjustable centrifugal turntable provided in this article can easily verify the influence of different centrifugal radii on the results of centrifugation experiments while avoiding batch-to-batch differences caused by changes in the centrifugal environment.
图1为一个血型检测用微流控芯片的形状示意图。Figure 1 is a schematic diagram of the shape of a microfluidic chip for blood group detection.
图2为本发明可调节式离心转盘的一个实施例的示意图。Figure 2 is a schematic diagram of an embodiment of the adjustable centrifugal turntable of the present invention.
图3为本发明可调节式离心转盘的另一实施例的示意图。Fig. 3 is a schematic diagram of another embodiment of the adjustable centrifugal turntable of the present invention.
图4为图3中可调节式离心转盘的部分放大图，显示了卡槽的结构。Fig. 4 is a partial enlarged view of the adjustable centrifugal turntable in Fig. 3, showing the structure of the card slot.
图5为利用固定销固定卡槽的示意图。Fig. 5 is a schematic diagram of using a fixing pin to fix the card slot.
图6为利用带有固定销的连接把手固定卡槽的示意图。Fig. 6 is a schematic diagram of using a connecting handle with a fixing pin to fix the card slot.
图7显示了带有突出部的卡槽与离心转盘上设置的径向槽的配合关系。Figure 7 shows the matching relationship between the card slot with the protrusion and the radial slot provided on the centrifugal turntable.
图8为利用本发明的可调节式离心转盘将不同卡槽定位于不同离心半径处的示意图。Fig. 8 is a schematic diagram of using the adjustable centrifugal turntable of the present invention to position different slots at different centrifugal radii.
图9为装有检测卡的离心转盘的局部放大图，显示了置于卡槽中的检测卡。Figure 9 is a partial enlarged view of the centrifugal turntable with the test card, showing the test card placed in the card slot.
除非另有说明，本文使用的所有技术和科学术语具有本领域普通技术人员所通常理解的含义。Unless otherwise stated, all technical and scientific terms used herein have the meanings commonly understood by those of ordinary skill in the art.
本文所用的“多个”包括两个及两个以上。As used herein, "plurality" includes two and more than two.
提及离心转盘时，所用的“可调节式”指，当将检测卡在该离心转盘上离心时，其离心半径可在离心前进行调整。相应地，当多个检测卡在该离心转盘上同时离心时，可在离心前分别调整它们的离心半径，使得至少两个检测卡的离心半径不同。显然地，也可调整这些检测卡的离心半径，使它们都相同。When referring to the centrifugal turntable, the "adjustable type" used means that when the test card is centrifuged on the centrifugal turntable, the centrifugal radius can be adjusted before centrifugation. Correspondingly, when multiple detection cards are centrifuged on the centrifuge turntable at the same time, their centrifugal radii can be adjusted respectively before centrifugation, so that the centrifugal radii of at least two detection cards are different. Obviously, the centrifugal radius of these test cards can also be adjusted to make them all the same.
“检测卡”指用于各种检测目的的卡状物，例如包括各种微型血型卡和微流控芯片。它们常见的形状为薄立方体。当然，其他形状或用途的检测卡也可以包括进来，只要其可方便地固定于本发明的离心转盘上或离心转盘所带的卡槽中。"Test card" refers to a card used for various testing purposes, including various miniature blood type cards and microfluidic chips, for example. Their common shape is a thin cube. Of course, detection cards of other shapes or uses can also be included, as long as they can be conveniently fixed on the centrifugal turntable of the present invention or in the card slot of the centrifugal turntable.
当提及检测卡定位模块时，所用的“对称设置”指多个检测卡定位模块相对于离心轴对称。When referring to the detection card positioning module, the "symmetrical arrangement" used means that multiple detection card positioning modules are symmetrical with respect to the centrifugal axis.
本文中提及的“定位孔”用于检测卡或卡槽的定位。为了实现该目的，也可以采用定位槽替换定位孔，同时对待与其配合连接的其他结构做适应性改动。为了阐述简洁，本文未另外对采用定位槽的实施方案进行描述，但显然它们也应包括在本发明的范围内。The "positioning hole" mentioned in this article is used to detect the positioning of the card or the card slot. In order to achieve this purpose, the positioning slot can also be used to replace the positioning hole, and other structures to be connected with it can be adapted to be modified at the same time. For the sake of brevity, this article does not additionally describe the embodiments using positioning grooves, but it is obvious that they should also be included in the scope of the present invention.
以下结合附图进一步详细说明本发明。Hereinafter, the present invention will be described in further detail with reference to the accompanying drawings.
在一个实例中，本发明的可调节式离心转盘如图2所示。其包括离心转盘本体1、设置在其上的安装孔2和6个检测卡定位模块3。离心转盘本体1可采用任何适合材料制作，例如金属、玻璃、塑料等。安装孔2用于离心转盘本体1与离心机转轴的固定。每个检测卡定位模块3包括多个成对的定位孔4。成对的定位孔4沿离心转盘本体1的半径间隔设置。间隔的距离可视实验要求而定，在大多数情况下可以考虑等距离设置。每对定位孔4之间的连线通常垂直于离心转盘的半径。当以不同的成对的定位孔4固定检测卡位置时，检测卡在离心时就具有不同的离心半径。为了方便将检测卡固定在离心转盘本体1上，一种方式是在检测卡上设置与成对的定位孔4对应的孔，并通过固定销来固定。另一种方式是通过在离心转盘本体1上设置用于容纳检测卡的卡槽，再通过固定卡槽来间接固定检测卡(见下文说明)。In an example, the adjustable centrifugal turntable of the present invention is shown in FIG. 2. It includes a centrifugal turntable body 1, a mounting hole 2 provided on it, and 6 detection card positioning modules 3. The centrifugal turntable body 1 can be made of any suitable material, such as metal, glass, plastic and so on. The mounting hole 2 is used for fixing the centrifugal turntable body 1 and the rotating shaft of the centrifuge. Each detection card positioning module 3 includes a plurality of pairs of positioning holes 4. The pair of positioning holes 4 are arranged at intervals along the radius of the centrifugal turntable body 1. The distance of the interval can be determined according to the requirements of the experiment. In most cases, equidistant settings can be considered. The line between each pair of positioning holes 4 is usually perpendicular to the radius of the centrifugal turntable. When different pairs of positioning holes 4 are used to fix the position of the detection card, the detection card will have different centrifugal radii during centrifugation. In order to facilitate the fixing of the detection card on the centrifugal turntable body 1, one way is to provide holes corresponding to the pair of positioning holes 4 on the detection card and fix them by fixing pins. Another way is to provide a card slot for accommodating the test card on the centrifugal turntable body 1, and then fix the test card indirectly by fixing the card slot (see description below).
在另一个实例中，本发明的可调节式离心转盘如图3所示。其包括离心转盘本体1、设置在其上的安装孔2和6个检测卡定位模块3’。每个检测卡定位模块3’包括卡槽5和多个成对的定位孔4。其中卡槽5上与所述定位孔4对应地设置有卡槽孔6(参见图4和图5)。可采用固定销7将卡槽5固定于不同离心半径的定位孔4。在另一种固定方式中，可不使用单独的固定销7，而是例如可直接在卡槽5下部设置两定位销，它们适合于与定位孔4配合来固定卡槽5。在可替代的固定方式中，还可以采用如图6所示的带有定位销结构的连接把手8来固定卡槽5。In another example, the adjustable centrifugal turntable of the present invention is shown in FIG. 3. It includes a centrifugal turntable body 1, a mounting hole 2 provided on it, and 6 detection card positioning modules 3'. Each detection card positioning module 3'includes a card slot 5 and a plurality of pairs of positioning holes 4. Wherein, the card slot 5 is provided with a card slot hole 6 corresponding to the positioning hole 4 (see FIGS. 4 and 5). The fixing pin 7 can be used to fix the slot 5 to the positioning holes 4 with different centrifugal radii. In another fixing method, a separate fixing pin 7 may not be used. For example, two positioning pins may be directly arranged under the slot 5, which are suitable for cooperating with the positioning hole 4 to fix the slot 5. In an alternative fixing manner, a connecting handle 8 with a positioning pin structure as shown in FIG. 6 can also be used to fix the card slot 5.
为了方便彼此固定连接，定位孔4、固定销7以及卡槽5上的卡槽孔6或者定位销可设置有螺纹，将连接方式由插销变成螺纹连接。在定位孔4带有内螺纹情况下，与其结合的固定销7也可以为螺钉形式。In order to facilitate the fixed connection with each other, the positioning hole 4, the fixing pin 7 and the slot hole 6 or the positioning pin on the slot 5 may be provided with threads, and the connection mode is changed from a plug pin to a threaded connection. In the case that the positioning hole 4 has an internal thread, the fixing pin 7 combined with it may also be in the form of a screw.
为了方便卡槽5沿离心转盘本体1径向移动，可在离心转盘本体1上在两排定位孔4之间设置径向槽9，并在卡槽5下方设置突出部10(见图7)。通常，径向槽9的宽度与突出部10的宽度匹配。径向槽9的设置还可以用于调整离心转盘的整体重量。In order to facilitate the radial movement of the slot 5 along the centrifugal turntable body 1, a radial slot 9 can be provided between the two rows of positioning holes 4 on the centrifugal turntable body 1, and a protrusion 10 can be provided under the slot 5 (see Figure 7) . Generally, the width of the radial groove 9 matches the width of the protrusion 10. The arrangement of the radial groove 9 can also be used to adjust the overall weight of the centrifugal turntable.
为了方便了解检测卡在各个位置所对应的离心半径，可在转盘本体1上在定位孔4旁边标记对应的离心半径值。In order to facilitate the understanding of the centrifugal radius corresponding to each position of the detection card, the corresponding centrifugal radius value can be marked on the turntable body 1 beside the positioning hole 4.
为了选择适合于检测卡进行检测的离心半径，可如图8所示，通过固定销7将多个卡槽5分别固定在离心转盘本体1上，并通过选择不同的定位孔4使得各卡槽5内 ***的检测卡11具有不同的离心半径(结合参见图9)。图9为装有检测卡11的离心转盘的局部放大图，显示了置于卡槽5中的检测卡11。按此方式，通过一次或少数几次离心实验就能确定适合的离心半径。离心时施加在检测卡的离心力与离心半径和离心机的转速有关，因而在固定离心半径情况下可通过简单换算而得到适合的离心转速。In order to select the centrifugal radius suitable for the detection of the test card, as shown in Fig. 8, a plurality of card slots 5 can be fixed on the centrifugal turntable body 1 by a fixing pin 7 respectively, and different positioning holes 4 can be selected to make each card slot 5 The detection card 11 inserted in 5 has different centrifugal radii (see Fig. 9 in combination). FIG. 9 is a partial enlarged view of the centrifugal turntable equipped with the detection card 11, showing the detection card 11 placed in the card slot 5. In this way, a suitable centrifugal radius can be determined through one or a few centrifugal experiments. The centrifugal force applied to the test card during centrifugation is related to the centrifugal radius and the speed of the centrifuge, so in the case of a fixed centrifugal radius, a suitable centrifugal speed can be obtained by simple conversion.
在同一次离心过程中，本发明可调节式离心转盘上固定的多个检测卡处于同一环境条件下，例如温度、湿度、光照等，消除了多次离心操作之间检测卡所处环境的差异。显而易见，由于无法保证两次离心环境完全相同，这种差异却是传统离心半径选择方式(改变转速或更换转盘)无法避免的。During the same centrifugation process, the multiple test cards fixed on the adjustable centrifugal turntable of the present invention are under the same environmental conditions, such as temperature, humidity, light, etc., which eliminates the difference in the environment where the test cards are located between multiple centrifugation operations . Obviously, since the two centrifugation environments cannot be guaranteed to be exactly the same, this difference is unavoidable in the traditional centrifugal radius selection method (change the speed or replace the turntable).
因此，利用本发明的可调节式离心转盘可以在同一批次内完成不同半径离心效果的实验，完成对照验证，节约时间，节约成本。Therefore, by using the adjustable centrifugal turntable of the present invention, experiments of centrifugal effects of different radii can be completed in the same batch, comparison verification can be completed, time and cost can be saved.
Claims (10)
- 可调节式离心转盘，包括离心转盘本体和设置在所述离心转盘本体上的多个定位模块，所述定位模块包括在离心半径方向依次设置的多个定位件，所述定位件用于确定离心时被离心件的离心半径。The adjustable centrifugal turntable includes a centrifugal turntable body and a plurality of positioning modules arranged on the centrifugal turntable body. When the centrifugal radius of the centrifugal piece.
- 如权利要求1所述的离心转盘，其中所述被离心件为检测卡。The centrifugal turntable according to claim 1, wherein the centrifuged part is a test card.
- 如权利要求1所述的离心转盘，其中所述多个定位模块在所述离心转盘上对称设置。The centrifugal turntable according to claim 1, wherein the plurality of positioning modules are symmetrically arranged on the centrifugal turntable.
- 如权利要求1所述的离心转盘，其中所述定位件为成对设置的定位孔。The centrifugal turntable according to claim 1, wherein the positioning members are positioning holes arranged in pairs.
- 如权利要求2所述的离心转盘，其中所述定位模块还包括用于容纳所述检测卡的卡槽；所述卡槽上设置有与所述定位件配合以在所述离心转盘上固定所述卡槽的卡槽定位结构。The centrifugal turntable according to claim 2, wherein the positioning module further comprises a card slot for accommodating the detection card; the card slot is provided with the positioning member for fixing the position on the centrifugal turntable. The card slot positioning structure of the card slot is described.
- 如权利要求5所述的离心转盘，其中所述卡槽定位结构为成对设置的卡槽定位孔或卡槽定位销。The centrifugal turntable according to claim 5, wherein the slot positioning structure is a pair of slot positioning holes or slot positioning pins.
- 如权利要求6所述的离心转盘，还包括用于将带有所述卡槽定位孔的所述卡槽固定在所述离心转盘上的多个固定销。7. The centrifugal turntable according to claim 6, further comprising a plurality of fixing pins for fixing the slot with the slot positioning hole on the centrifugal turntable.
- 如权利要求6所述的离心转盘，还包括用于将带有所述卡槽定位孔的所述卡槽固定在所述离心转盘上的多个带有两定位销的连接把手。The centrifugal turntable according to claim 6, further comprising a plurality of connecting handles with two positioning pins for fixing the slot with the positioning hole of the slot on the centrifugal turntable.
- 权利要求1-8任一项所述的可调节式离心转盘用于对微流控芯片进行离心的应用。The adjustable centrifugal turntable of any one of claims 1-8 is used for centrifuging the microfluidic chip.
- 包括权利要求1-8任一项所述的可调节式离心转盘的离心机。A centrifuge comprising the adjustable centrifugal turntable of any one of claims 1-8.
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| CN111530641A (en) * | 2020-05-07 | 2020-08-14 | 天津德祥生物技术有限公司 | Adjustable centrifugal turntable and application thereof |
| CN112354690B (en) * | 2020-11-18 | 2022-07-26 | 天津德祥生物技术有限公司 | Rotor of centrifugal machine |
| CN113019717B (en) * | 2021-02-04 | 2022-10-11 | 赛赋(北京)检测技术服务有限公司 | Stem cell centrifugal equipment with adjust structure and function |
| CN113083519A (en) * | 2021-03-31 | 2021-07-09 | 中国人民解放军空军军医大学 | Overweight simulation centrifuge |
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