WO2021189453A1 - 一种微型荧光显微成像模块 - Google Patents
一种微型荧光显微成像模块 Download PDFInfo
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- WO2021189453A1 WO2021189453A1 PCT/CN2020/081796 CN2020081796W WO2021189453A1 WO 2021189453 A1 WO2021189453 A1 WO 2021189453A1 CN 2020081796 W CN2020081796 W CN 2020081796W WO 2021189453 A1 WO2021189453 A1 WO 2021189453A1
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- lens
- objective lens
- excitation light
- light source
- fluorescence microscopy
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- 238000003384 imaging method Methods 0.000 title claims abstract description 82
- 238000000799 fluorescence microscopy Methods 0.000 title claims abstract description 45
- 230000005284 excitation Effects 0.000 claims abstract description 83
- 238000005286 illumination Methods 0.000 claims abstract description 36
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
Definitions
- the invention relates to the field of optical imaging, in particular to a miniature fluorescent microscopic imaging module.
- Fluorescence microscope In the field of life sciences, fluorescence microscopes are often used. Fluorescence microscope usually includes light source, excitation filter, dichroic lens, objective lens, emission filter, tube lens, detector and other devices.
- the light source passes through the excitation filter to obtain the excitation light, which is reflected by the dichroic mirror into the objective lens, and is focused on the sample surface.
- the excited fluorescence signal is collected by the objective lens, and then imaged by the tube lens to the detector target surface after passing through the dichroic mirror and the filter.
- This kind of microscope usually has a certain magnification, which will inevitably result in short object distance and long image distance. There must be enough space in the image space to meet the requirements of this optical path setting.
- the light source needs to be connected to the mains power
- the detectors such as CCD or CMOS also need to be connected to the mains or computer power supply
- the image display and storage also need to be connected to the computer.
- the microscope must also have a focusing mechanism. Whether it is manual focusing or automatic focusing, its mechanical structure is relatively complicated and takes up a lot of space. For these reasons, fluorescence microscopes are usually large in size, complex in structure, high in cost, and not portable.
- the core components that can be imaged by the fluorescence microscope are the objective lens and the tube lens.
- the main factor restricting its inability to miniaturize is the large magnification of the system, which results in the long focal length of the tube lens.
- the filter set still uses the previous settings for miniaturization, without further consideration of its miniaturization design.
- the technical problem to be solved by the present invention is to provide a miniature fluorescent microscopic imaging module in view of the defects of the prior art.
- the technical solution adopted by the present invention to solve its technical problem is: constructing a miniature fluorescent microscopic imaging module, including an imaging lens module and an illumination device;
- the imaging lens module includes an objective lens and a tube lens arranged symmetrically from the object side to the image side, an image sensor with a high spatial sampling rate arranged on the side of the tube mirror, and an image sensor arranged in front of the objective lens, or The emission filter between the objective lens and the tube lens, or between the tube lens and the image sensor;
- the illumination device is arranged separately from the imaging lens module to form uniform illumination of the sample
- the excitation light path of the illumination device is set separately from the detection light path of the imaging lens module; the detection light path from the object side to the image side is the sample, the emission filter, the objective lens, the tube lens and the image sensor; or the sample, Objective lens, emission filter, tube lens and image sensor; or sample, objective lens, tube lens, emission filter and image sensor.
- the objective lens and the tube lens form a lens group, and the distance from the object surface of the lens group to the image surface on the optical axis is TTL, and the The distance from the object side surface of the lens group to the image side surface on the optical axis is TD, which satisfies the following relationship: 0.15 ⁇ TD/TTL ⁇ 0.9;
- the length of the optical tube of the lens group satisfies the following relationship: 0.2 ⁇ TTL/( f 1 +f 2 ) ⁇ 15;
- the distance L Obj from the principal plane of the object side of the objective lens to the object surface satisfies the following relationship: 0.5f 1 ⁇ L Obj ⁇ 1.5f 1 ;
- the distance L Ima from the principal plane of the image side of the tube lens to the image surface satisfies the following relationship: 0.5f 2 ⁇ L Ima ⁇ 1.5f 2 .
- the illumination device includes an excitation light source arranged on the periphery of the objective lens and a uniform light guide plate corresponding to the excitation light source;
- the light emitted by the excitation light source passes through the uniform light guide plate to form uniform illumination of the sample.
- the excitation light source includes a plurality of excitation light sources, which are uniformly distributed on the periphery of the objective lens in a ring shape; And the end surface of the ring-shaped light guide plate is substantially flush with the end surface of the objective lens close to the sample.
- the illumination device includes an excitation light source arranged on one side of the sample; the light emitted by the excitation light source illuminates the target area from the side of the sample .
- the micro-fluorescence microscopy imaging module further includes a support arm provided on one side of the imaging lens module; the excitation light source is mounted on the support arm .
- the illumination device is arranged under the sample, and includes a first excitation light source arranged correspondingly under the periphery of the objective lens, and the first excitation light source.
- the first uniform light guide plate corresponding to the excitation light source and the shielding baffle set corresponding to the edge of the field of view of the objective lens, the light is irradiated to the target area from below the sample side.
- the lighting device further includes a second excitation light source correspondingly arranged under the field of view of the objective lens, and a second excitation light source corresponding to the second excitation light source.
- Uniform light guide plate the light is irradiated to the target area from directly below the sample;
- the shielding baffle separates the first excitation light source and the second excitation light source, the first uniform light guide plate and the second uniform light guide plate, respectively.
- the micro-fluorescence microscopy imaging module further includes a support base, the support base includes a support arm, and a base fixed at the end of the support arm; The support arm fixes the sample from its side.
- the excitation light source is a laser diode or a light emitting diode
- the objective lens and the tube lens are respectively composed of at least three lenses.
- the miniature fluorescence microscope imaging module further includes a drive circuit board with a USB interface for driving the illumination device and the image sensor; and or
- a protective glass set in front of the objective lens is
- the micro-fluorescence microscopy imaging module further includes a focusing motor, with an animal lens, or a tube lens, or is composed of an objective lens, an emission filter, and a tube lens The overall movement to achieve focusing.
- a focusing motor with an animal lens, or a tube lens, or is composed of an objective lens, an emission filter, and a tube lens The overall movement to achieve focusing.
- the invention is based on the structure of a traditional infinity-corrected fluorescence microscope, adopts an approximately symmetrical structure to design an objective lens and a tube lens, and combines a sensor with a high spatial sampling rate to realize high-resolution imaging at a low magnification.
- This design can effectively shorten the conjugation distance and solve the problem that the image distance is still very long after the microscope is miniaturized.
- the three excitation lighting schemes eliminate the need to add excitation filters and dichroic mirrors, which can meet the lighting requirements for different observation samples.
- the three different positions of the emission filter reduce the aberration and ensure the imaging quality.
- the driving circuit of the entire microscope can be powered by a simple USB connection, and the image of the image sensor can also be connected to a computer or mobile phone through the USB for display and storage.
- the microscope imaging module can also add a focusing motor. Due to the small size and light weight of the objective lens and tube lens, mature focusing motors such as voice coil motors can be used to drive the lens group to focus.
- Fig. 1 is a schematic structural diagram of a first embodiment of a miniature fluorescence microscopy imaging module of the present invention
- Fig. 2 is a schematic structural diagram of a second embodiment of the miniature fluorescence microscopy imaging module of the present invention.
- Fig. 3 is a schematic structural diagram of a third embodiment of a miniature fluorescence microscopy imaging module of the present invention.
- Fig. 4 is a schematic structural diagram of a fourth embodiment of the miniature fluorescence microscopy imaging module of the present invention.
- orientation or positional relationship indicated by “front”, “rear”, “upper”, “lower”, etc. is based on the orientation or positional relationship shown in the drawings, constructed and operated in a specific orientation, and is only for It is convenient to describe the technical solution, rather than indicating that the pointed device or element must have a specific orientation, so it cannot be understood as a limitation of the present utility model.
- the present invention constructs a miniature fluorescent microscopy imaging module, which includes an imaging lens module and an illumination device;
- the imaging lens module includes an objective lens 18 and a tube lens 16 arranged symmetrically from the object side to the image side, an image sensor 28 with a high spatial sampling rate arranged on the image side of the tube lens 16, and an image sensor 28 arranged in front of the objective lens 18, or The emission filter 17 between the objective lens 18 and the tube lens 16 or between the tube lens 16 and the image sensor 28.
- the illuminating device and the imaging lens module are separately arranged to form uniform illumination of the sample 10.
- the excitation light path of the illumination device is set separately from the detection light path of the imaging lens module; the detection light path from the object side to the image side is the sample 10, the emission filter 17, the objective lens 18, the tube lens 16 and the image sensor 28; or the sample 10, The objective lens 18, the emission filter 17, the barrel lens 16 and the image sensor 28; or the sample 10, the objective lens 18, the barrel lens 16, the emission filter 17 and the image sensor 28.
- the objective lens 18 and the tube lens 16 are respectively composed of at least three lenses.
- the material of the lens can be glass or plastic, and the surface of the lens can be set to a spherical or aspherical surface according to aberration correction requirements.
- the objective lens 18 and the tube lens 16 form a lens group.
- the lens group is an infinity-corrected microscope and adopts an approximately symmetrical design to facilitate optimization of aberrations. Under low magnification, a resolution of 1 ⁇ m can be achieved. Low magnification can effectively reduce the length of the image distance, realize the miniaturization of the microscope, and can increase the field of view.
- the distance from the object surface of the lens group to the image surface on the optical axis is TTL
- the distance from the object side surface of the lens group to the image side surface on the optical axis is TD, which satisfies the following relationship: 0.15 ⁇ TD/TTL ⁇ 0.9 ;
- the optical tube length of the lens group satisfies the following relationship: 0.2 ⁇ TTL/( f 1 +f 2 ) ⁇ 15;
- the distance L Obj from the principal plane of the object side of the objective lens 18 to the object surface satisfies the following relationship: 0.5f 1 ⁇ L Obj ⁇ 1.5f 1 ;
- the distance L Ima from the principal plane of the image side of the tube lens 16 to the image surface satisfies the following relationship: 0.5f 2 ⁇ L Ima ⁇ 1.5f 2 .
- the emission filter 17 may be an absorption filter or an interference filter.
- the distance between the objective lens 18 and the tube lens 16 can be shortened, which helps light
- the pupil is connected to better correct aberrations; when it is set between the objective lens 18 and the tube lens 16, because the light incident on the filter is parallel light, aberrations can be avoided; it is set on the tube lens 16 and the image sensor When it is between 28, the light incident angle is small when working in afocal mode, which is beneficial to better realize the function of filtering light.
- the micro fluorescence microscopy imaging module further includes a driving circuit board 11 with a USB interface for driving the lighting device, the image sensor 28 and the focusing motor 27. Its USB interface can be connected to computers, mobile phones and other devices to achieve power supply and display images.
- the lighting device includes an excitation light source 19 and a uniform light guide plate 29 corresponding to the excitation light source 19.
- the excitation light source 19 can be a laser diode or a light emitting diode
- the material of the homogenizing light guide plate 29 can be PC (polycarbonate), PMMA (acrylic), glass and other materials, and the homogenization effect is achieved by processing the surface of the material into a frosted surface.
- the illumination method of the illuminating device is as follows: 1) Annular light reflective dark field illumination above the sample, the annular light is distributed on the periphery of the objective lens and is flush with the lower surface of the objective lens;
- the side of the sample is illuminated, and the light from the 0° direction of the light source is directed to the center of the sample field of view within the range of an angle of 10° with the horizontal plane.
- the light from the 0° light emitting direction of the light source is incident on the center of the sample field of view along the horizontal direction;
- the ring light is distributed under the sample and outside the field of view.
- the above three illumination methods can achieve uniform illumination of the sample surface while avoiding the excitation light directly entering the lens to form stray light, thereby affecting the imaging effect.
- the three lighting methods eliminate the configuration of dichroic mirrors and excitation filters, which can effectively reduce the space and increase the compactness of the structure.
- a protective glass 15 is provided in front of the objective lens 18, which can effectively protect the entire imaging module from external dust, moisture and other pollution.
- the structure is miniature, compact, simple and easy to install and adjust. Because the microscope adopts an approximately symmetrical structure, combined with a high spatial sampling rate image sensor, a large field of view and high resolution imaging can be realized at low magnification, which solves the problem of long image distance, which is beneficial to shorten the conjugate distance and realize imaging Miniaturization of modules. In addition, the aforementioned excitation filter and dichroic mirror do not need to be provided, which simplifies the structure and further reduces the space occupied, thereby realizing a more compact design.
- the fluorescence microscopy imaging module only needs to be powered by USB to work, saving the trouble of connecting to the mains.
- it can be directly connected to a mobile phone to display and store images, which is convenient to carry and use outdoors.
- the micro-fluorescence microscopy imaging module may further include a focusing motor 27, and optionally the focusing motor 27 may be a voice coil motor, a stepping motor, an ultrasonic motor, a memory alloy motor, and the like.
- the focusing motor 27 can carry the objective lens 18 to achieve focusing without affecting the magnification of the system. If the influence on the magnification of the system is not considered, the focusing motor 27 can also drive the barrel lens 16 or the overall movement composed of the objective lens 18, the emission filter 17, and the barrel lens 16 to achieve focusing.
- the focusing motor 27 When the focusing motor 27 is equipped with the animal lens 18 or tube lens 16 to realize focusing, it changes the position of the object plane of the miniature fluorescence microscopy imaging module.
- the object plane closest to the micro-fluorescence microscopy imaging module is the near-focus object plane
- the object plane farthest from the micro-fluorescence microscopy imaging module is the far-focus object plane
- the direction away from the imaging lens module is the positive direction.
- the objective lens 18 and the tube lens 16 form a lens group, which includes a limit surface on the object side of the lens group.
- the object side surface of the protective glass 15 is the limit surface;
- the end surface of the shell of the imaging lens module or other mechanical structure matched with the imaging lens module is the limit surface.
- the near-focus object surface is within ⁇ 50 ⁇ m of the limit surface, and the distance between the far-focus object surface and the limit surface is ⁇ 220 ⁇ m.
- the stroke of the focusing motor 27 is ⁇ 300 ⁇ m and ⁇ 600 ⁇ m, and when the focusing motor 27 moves with the objective lens 18, the focusing distance is the same as the stroke of the focusing motor 27.
- the minimum distance between the objective lens 18 and the tube lens 16 is ⁇ 50 ⁇ m.
- the minimum distance between the objective lens 18 and the protective glass 15 is ⁇ 50 ⁇ m.
- the near-focus object surface can cover the short-distance area of the protective glass 15 and can image close objects. At the same time, the far-focus object surface can surpass the cover glass commonly used in microscopes to meet the needs of biomedical imaging;
- the stroke of the focusing motor 27 can effectively cover the dimensional tolerances of the components in the module due to processing and installation, which improves the manufacturability of mass production;
- the near-focus object surface at this time is the object surface that the lens group focuses on when the distance between the objective lens 18 and the tube lens 16 is the smallest;
- the near-focus object plane at this time is the object plane on which the lens group focuses when the distance between the objective lens 18 and the tube lens 16 is the smallest.
- the detection light path from the object side to the image side is the sample 10, the protective glass 15, the objective lens 18, the tube lens 16, the emission filter 17, and the image sensor 28 in order.
- the objective lens 18 is fixed in the voice coil motor 27, and the tube lens 16 is fixed in the tube lens fixing member 12.
- the distance TTL from the object surface to the image surface of the lens group on the optical axis is 10.2 mm
- the distance TD from the object side surface to the image side surface on the optical axis is 7.2 mm.
- the emission filter 17 is fixed in the housing 26, and the image sensor 28 is directly integrated on the driving circuit board 11.
- the lighting device includes an excitation light source 19 arranged on the periphery of the objective lens 18 and a uniform light guide plate 29 corresponding to the excitation light source 19.
- the light emitted by the excitation light source 19 passes through the uniform light guide plate 29 to form uniform illumination for the sample 10.
- the excitation light source 19 includes a plurality of excitation light sources 19, which are uniformly distributed around the periphery of the objective lens 18 in a ring shape.
- the end surface of 18 close to the sample 10 is basically flush, forming a ring-shaped light reflection dark field illumination above the sample.
- the excitation light source 19 is an LED lamp, and the LED lamp and the uniform light guide plate 29 are fixed on the housing 26.
- the LED lamp is at least two LED light sources distributed at equal angular intervals along the circumferential direction. The specific number of light sources can be illuminated according to the sample surface. Parameter settings such as uniformity and illuminance value.
- USB interface on the driving circuit board 11, which can be directly connected to a computer USB port or connected to a mobile phone through an OTG cable.
- the LED lamp 19 emits excitation light, which irradiates the sample 10 through the uniform light guide plate 29 and the protective glass 15 to form uniform illumination.
- the fluorescent substance is excited to generate a fluorescent signal, which is collected by the objective lens 18 after passing through the protective glass 15, and then sequentially focused by the tube lens 16 and filtered by the emission filter 17, and finally imaged on the image sensor 28.
- the signal of the image sensor 28 is processed by the driving circuit board 11, and displayed on a computer or a mobile phone via a USB data line, which allows the operator to view the fluorescent image in real time and save it as needed.
- the voice coil motor 27 is fixed to the housing 26 around the objective lens 18.
- the driving circuit board 11 drives the voice coil motor 27, and the energized coil inside the voice coil motor 27 generates ampere force under the action of the magnetic field, so that the objective lens 18 can move axially, realizing the automatic focusing function.
- the lighting device in the second embodiment, as shown in FIG. 2, compared with the first embodiment, the lighting device, the position of the emission filter 17 and the setting of the focusing object are different.
- the illuminating device includes an excitation light source 19 arranged on the side of the sample 10, and light emitted by the excitation light source 19 is irradiated from the side of the sample 10 to the target area.
- the miniature fluorescence microscopy imaging module also includes a support arm 24 arranged on one side of the imaging lens module, and the excitation light source 19 is mounted on the support arm 24.
- the micro-fluorescence microscopy imaging module further includes a support base, and the support base includes a support arm 24 and a base 23 fixed at the end of the support arm 24.
- the excitation light source 19 is a light-emitting diode, which is fixed on the support arm 24, and the light emitted from it illuminates the target area from the side of the sample.
- the structure is simple and can achieve the effect of uniform illumination.
- the number of excitation light sources 19 can be distributed along the periphery of the sample according to actual lighting requirements.
- Support arms 24 are connected to the drive circuit board 11 to provide circuit support for the excitation light sources 19.
- the base 23 provides support for the entire fluorescence microscopy imaging module.
- the emission filter 17 is arranged between the objective lens 18 and the tube lens 16 and fixed on the housing 26.
- the emission filter 17 filters out other unwanted wavelengths of light passing through the objective lens 18 to avoid aberrations.
- the voice coil motor 27 is fixed on the housing 26 around the tube lens 16 to drive the tube lens 16 to move axially to realize automatic focusing.
- the lighting device, the position of the emission filter 17, and the setting of the focusing object are different.
- the illuminating device is arranged under the sample 10, and includes at least two first excitation light sources 19 corresponding to the periphery of the field of view of the objective lens 18, a first uniform light guide plate 29 corresponding to the first excitation light source 19, and a field of view corresponding to the objective lens 18
- the shielding plate 30 is set at the edge, and light is irradiated to the target area from the bottom of the sample 10 side.
- the lighting device also includes at least one second excitation light source 19A correspondingly arranged under the field of view of the objective lens 18 and a second uniform light guide plate 29A corresponding to the second excitation light source 19A.
- the shielding plate 30 separates the first excitation light source 19 and the second excitation light source 19A, the first uniform light guide plate 29 and the second uniform light guide plate 29A, respectively.
- the excitation light source includes a plurality of excitation light sources, which are uniformly distributed in the outer periphery of the field of view of the objective lens 18 in a ring shape.
- the uniform light guide plate is an annular light guide plate arranged in the light emission direction of the excitation light source.
- the micro-fluorescence microscopy imaging module further includes a support base, and the support base includes a support arm 24 and a base 23 fixed at the end of the support arm 24.
- the support arm 24 fixes the sample 10 from its side.
- the first excitation light source 19 and the second excitation light source 19A are both LED lamps.
- the LED lamps are fixed on the base 23 and located at the periphery of the field of view of the objective lens.
- the lower part of the sample side illuminates the target area to form a transmitted dark field illumination.
- This illumination method is suitable for uniform illumination of transparent samples, and can also prevent the illumination light from directly entering the objective lens.
- the function of the shielding plate 30 is to prevent the light of the LED lamp 19 from being directly incident on the sample surface from the inside, and at the same time to isolate the first uniform light guide plate 29 and the second uniform light guide plate 29A.
- directly below the sample there are also LED lights 19A, which can be independently controlled with the LED lights 19 respectively.
- the support arm 24 is connected to the driving circuit board 11 and provides circuit support for the ring-shaped light source, that is, the first excitation light source 19 and the second excitation light source 19A.
- the emission filter 17 is arranged in front of the objective lens 18, which can shorten the distance between the objective lens 18 and the tube lens 16, facilitate pupil connection, and better correct aberrations.
- the position where the voice coil motor 27 is set is different.
- the voice coil motor 27 is located on the periphery of the fixed parts of the objective lens 18, the tube lens 16, and the emission filter 17, which can drive the lens group to move as a whole to realize automatic focusing.
- the voice coil motor 27 is reduced, and the objective lens 18 is fixed on the objective lens fixing member 13, so that the structure of the entire microscope is simpler and miniaturized.
- the protective glass 15 is reduced to reduce the stray light entering the lens.
- the light source 22 is a laser diode, which has a small divergence angle, and uses light to be fully concentrated and incident on the surface of the sample.
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Abstract
Description
Claims (12)
- 一种微型荧光显微成像模块,其特征在于,包括成像镜头模组以及照明装置;所述成像镜头模组包括从物方至像方依次对称设置的物镜(18)和筒镜(16)、设于所述筒镜(16)像侧的具有高空间采样率的图像传感器(28)、以及设于所述物镜(18)前方、或所述物镜(18)与所述筒镜(16)之间、或所述筒镜(16)与所述图像传感器(28)之间的发射滤光片(17);所述照明装置与所述成像镜头模组分开设置,形成对样本(10)的均匀照明;所述照明装置的激发光路与所述成像镜头模组的探测光路分开设置;所述探测光路从物方到像方依次为样本(10)、发射滤光片(17)、物镜(18)、筒镜(16)和图像传感器(28);或样本(10)、物镜(18)、发射滤光片(17)、筒镜(16)和图像传感器(28);或样本(10)、物镜(18)、筒镜(16)、发射滤光片(17)和图像传感器(28)。
- 根据权利要求1所述的微型荧光显微成像模块,其特征在于,所述物镜(18)和所述筒镜(16)组成镜头组,所述镜头组的物面至像面于光轴上的距离为TTL,所述镜头组的物侧表面至像侧表面于光轴上的距离为TD,其满足如下关系:0.15≤TD/TTL≤0.9;所述物镜(18)的焦距f 1和所述筒镜(16)的焦距f 2之间满足如下关系:0.1≤f 1/f 2≤10;所述镜头组的光学筒长满足如下关系:0.2≤TTL/( f 1+f 2)≤15;所述物镜(18)的物方主平面至所述物面的距离L Obj满足如下关系:0.5f 1≤L Obj≤1.5f 1;所述筒镜(16)的像方主平面到所述像面的距离L Ima满足如下关系式:0.5f 2≤L Ima≤1.5f 2。
- 根据权利要求1所述的微型荧光显微成像模块,其特征在于,所述照明装置包括设置在所述物镜(18)***的激发光源(19)、以及与所述激发光源(19)对应的匀光导光板(29);所述激发光源(19)发出的光线经所述匀光导光板(29)后形成对所述样本(10)的均匀照明。
- 根据权利要求3所述的微型荧光显微成像模块,其特征在于,所述激发光源(19)包括多个,并呈环形均匀分布在所述物镜(18)***;所述匀光导光板(29)为设置在所述激发光源(19)的出光方向上的环形导光板;并且所述环形导光板的端面与所述物镜(18)靠近所述样本(10)的端面基本齐平。
- 根据权利要求1所述的微型荧光显微成像模块,其特征在于,所述照明装置包括设置在所述样本(10)一侧的激发光源(19);所述激发光源(19)发出的光线从所述样本(10)侧方照射到目标区域。
- 根据权利要求5所述的微型荧光显微成像模块,其特征在于,微型荧光显微成像模块还包括设置在所述成像镜头模组一侧的支撑臂(24);所述激发光源(19)安装在所述支撑臂(24)上。
- 根据权利要求1所述的微型荧光显微成像模块,其特征在于,所述照明装置设置于所述样本(10)下方,包括对应设置于所述物镜(18)视场***下的第一激发光源(19)、与所述第一激发光源(19)对应的第一匀光导光板(29)、以及对应于所述物镜(18)视场边缘设置的遮挡板(30),光线从所述样本(10)侧下方照射到目标区域。
- 根据权利要求7所述的微型荧光显微成像模块,其特征在于,所述照明装置还包括对应设置于所述物镜(18)视场下的第二激发光源(19A)、以及与所述第二激发光源(19A)对应的第二匀光导光板(29A),光线从所述样本(10)正下方照射到目标区域;所述遮挡板(30)分别将所述第一激发光源(19)和所述第二激发光源(19A)、所述第一匀光导光板(29)和所述第二匀光导光板(29A)隔离。
- 根据权利要求8所述的微型荧光显微成像模块,其特征在于,所述微型荧光显微成像模块还包括支撑座,所述支撑座包括支撑臂(24)、以及固定在所述支撑臂(24)末端的底座(23);所述支撑臂(24)从所述样本(10)侧方对其进行固定。
- 根据权利要求3-9任一项所述的微型荧光显微成像模块,其特征在于,所述激发光源(19)为激光二极管或发光二极管;所述物镜(18)和所述筒镜(16)分别由至少三片透镜组成。
- 根据权利要求1-9任一项所述的微型荧光显微成像模块,其特征在于,所述微型荧光显微成像模块还包括带有USB接口的驱动电路板(11),用于驱动所述照明装置和所述图像传感器(28);和或设于所述物镜(18)前方的保护玻璃(15)。
- 根据权利要求1所述的微型荧光显微成像模块,其特征在于,所述微型荧光显微成像模块还包括调焦马达(27),带动物镜(18)、或筒镜(16)、或由物镜(18)、发射滤光片(17)和筒镜(16)组成的整体移动以实现调焦。
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