WO2020258734A1 - Circularly polarized soliton generating device and multiphoton microscopic imaging system - Google Patents
Circularly polarized soliton generating device and multiphoton microscopic imaging system Download PDFInfo
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- WO2020258734A1 WO2020258734A1 PCT/CN2019/123620 CN2019123620W WO2020258734A1 WO 2020258734 A1 WO2020258734 A1 WO 2020258734A1 CN 2019123620 W CN2019123620 W CN 2019123620W WO 2020258734 A1 WO2020258734 A1 WO 2020258734A1
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
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- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/28—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for polarising
- G02B27/286—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for polarising for controlling or changing the state of polarisation, e.g. transforming one polarisation state into another
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- the invention relates to the technical field of optical imaging, in particular to a circularly polarized soliton generator and a multiphoton microscopic imaging system.
- Soliton refers to light pulses that are transmitted over a long distance while keeping their shape unchanged.
- a beam of light pulse contains many different frequency components, the frequency is different, and the propagation speed in the medium is also different. Therefore, the light pulse will be dispersed in the optical fiber, making the pulse width wider.
- the Kerr effect will occur, that is, the refractive index of the medium changes with the light intensity, which leads to self-phase modulation in the light pulse, making the pulse front
- the resulting phase change causes the frequency to decrease, and the phase change produced by the trailing edge of the pulse causes the frequency to rise, so the leading edge of the pulse propagates slower than the trailing edge, thus narrowing the pulse width.
- the pulse has an appropriate amplitude, the above two effects can be exactly offset, and the pulse can be transmitted in the fiber with a stable waveform, that is, an optical soliton is formed, which is also called a fundamental-order optical soliton.
- the pulse amplitude continues to increase, the narrowing effect will exceed the widening effect, and a high-order optical soliton will be formed.
- the pulse shape transmitted in the fiber will change continuously. First, it will be compressed and narrowed, then split, and the pulse will be periodic at a certain distance. To restore.
- Optical soliton is formed by two basic physical phenomena in optical fiber, namely group velocity dispersion (GVD) and self-phase modulation (SPM).
- GMD group velocity dispersion
- SPM self-phase modulation
- ⁇ d 1310nm is the zero-dispersion wavelength
- ⁇ > ⁇ d is called the abnormal dispersion region
- ⁇ d is called the normal dispersion region.
- the transmission characteristics of light pulses in the normal and abnormal dispersion regions are different.
- the abnormal dispersion region the high frequency components (blue shift) and lower frequency components (red shift) of the light pulse are transmitted faster, while in the normal dispersion region, the situation is just the opposite. . Due to different transmission conditions, the group velocity dispersion effect is different, which ultimately leads to the broadening of the optical pulse.
- the analysis of Gaussian pulses shows that self-phase modulation will cause a red shift in the leading edge of the pulse and a blue shift in the trailing edge.
- the analysis of pulses of other shapes has similar results.
- the high-frequency (blueshift) component of the pulse moves faster than the low-frequency (redshift) component
- the leading edge spectrum of the pulse caused by the self-phase modulation (SPM) effect Red shift slows down the movement of the leading edge of the pulse and speeds up the movement of the trailing edge of the pulse due to spectral blue shift, which makes the pulse narrower, which corresponds to the trend of pulse broadening in the anomalous dispersion region with group velocity dispersion.
- optical soliton is the precise balance of group velocity dispersion and self-phase modulation effect in the anomalous dispersion region in the fiber.
- the optical soliton has the following characteristics: ultra-short pulse width (tens of femtoseconds to sub-picosecond order), excellent pulse quality and tunable broadband wavelength Sex. These characteristics make it an ideal light source choice for multiphoton microscopy (MPM).
- MPM multiphoton microscopy
- Multiphoton microscopy imaging is a nonlinear optical imaging technique, especially suitable for deep tissue imaging in living body.
- Multiphoton microscopy imaging technology has been widely used in biology, physiology and medical research in different modalities.
- the main purpose of the present invention is to provide a circularly polarized soliton generating device and a multiphoton microscopic imaging system, aiming to solve the technical problem of low energy of the optical soliton generated in the prior art.
- a circularly polarized soliton generator includes a pump laser, a first quarter wave plate, a first focusing lens, and an optical fiber on the same optical path, wherein the linearly polarized light generated by the pump laser passes through the first quarter One of the wave plates is transformed into circularly polarized light, the circularly polarized light is coupled into the optical fiber after passing through the focusing lens, and the circularly polarized light is transformed into a circularly polarized soliton after passing through the optical fiber.
- the circularly polarized soliton generating device further includes a first collimating lens and a long-pass filter, wherein the circularly polarized soliton is scattered after passing through the collimating lens, and the scattered circularly polarized soliton passes through the long-pass filter. The light sheet is filtered.
- the optical fiber is a rod-shaped photonic crystal optical fiber.
- a multiphoton microscopic imaging system comprising a second quarter wave plate, a flat field focusing lens, a dichroic mirror, an objective lens, a photomultiplier tube, and the circularly polarized soliton generator according to any one of claims 1-3 Device; wherein, the circularly polarized soliton generated by the circularly polarized soliton generator is transformed into a linearly polarized soliton after passing through the second quarter wave plate, and the linearly polarized soliton passes through the flat field focusing lens and the dichroic
- the rear lens is coupled to the objective lens, and the signal is generated by focusing on the object to be detected through the objective lens. The signal is scattered and then passes through the objective lens and the dichroic mirror, and the dichroic mirror couples the signal to the photomultiplier Inside the tube and imaged through the photomultiplier tube.
- the multiphoton microscopic imaging system further includes a first reflector, and the first reflector is located between the second quarter wave plate and the plan focusing lens.
- the multiphoton microscopic imaging system further includes a tube lens, and the tube lens is located between the plan focusing lens and the dichroic mirror.
- the multiphoton microscopy imaging system further includes a second reflector, and the second reflector is located between the tube lens and the dichroic mirror.
- the above-mentioned circularly polarized soliton generator and multiphoton microscopic imaging system pass the first quarter-wave plate to convert linearly polarized light into circularly polarized light, and then turn the circularly polarized light into circularly polarized soliton after passing through an optical fiber.
- the energy of the circularly polarized soliton is 1.56 times the energy of the linearly polarized soliton, which enables multiphoton signals to be transmitted farther in the brain, so that humans can further explore the deep tissues of the brain and untie it. The mysterious veil of the brain.
- Fig. 1 is a schematic diagram of a circularly polarized soliton generator according to an embodiment of the present invention.
- Fig. 2 is a comparison diagram of the energy of a circularly polarized soliton and a linearly polarized soliton passing through a long-pass filter according to an embodiment of the present invention.
- Fig. 3(a) is a graph of the relationship between the normalized pump power of the linearly polarized light passing through the first quarter wave plate and the rotation angle of the polarizer according to an embodiment of the present invention.
- Fig. 3(b) is a diagram of the relationship between the normalized pump power of the circularly polarized light passing through the collimator lens and the rotation angle of the polarizer according to an embodiment of the present invention.
- Fig. 4 is a schematic diagram of a multiphoton microscopy imaging system according to an embodiment of the present invention.
- Multiphoton microscopy imaging system 1. Circular polarization soliton generator; 11. Pump laser; 12. First quarter wave plate; 13. First focusing lens; 14. Optical fiber; 15. First alignment Straight lens; 16. Long wave pass filter; 2. Second quarter wave plate; 3. Plan focusing lens; 31. Scanning mirror; 32. Scanning lens; 4. Dichroic mirror; 5. Objective; 6. Photomultiplier tube; 7. First reflector; 8. Tube lens; 9. Second reflector.
- Fig. 1 is a schematic diagram of a circularly polarized soliton generator according to an embodiment of the present invention.
- the circularly polarized soliton generator 1 may have a pump laser 11, a first quarter wave plate 12, a first focusing lens 13, and an optical fiber 14 on the same optical path, wherein the pump laser
- the linearly polarized light generated by 11 is transformed into circularly polarized light after passing through the first quarter wave plate 12, and the circularly polarized light is coupled into the optical fiber 14 after passing through the focusing lens, and the circularly polarized light is transformed into circularly polarized soliton after passing through the optical fiber 14.
- the linearly polarized light is changed to circularly polarized light through the first quarter wave plate 12, and then the circularly polarized light passes through the optical fiber 14 and becomes a circularly polarized soliton.
- the energy of the circularly polarized soliton is 1.56 times the energy of the linearly polarized soliton, which enables multiphoton signals to be transmitted farther in the brain, so that humans can further explore the deep tissues of the brain and untie it. The mysterious veil of the brain.
- i an imaginary unit, representing a differential symbol
- z represents a transmission distance
- Ax represents a linearly polarized pulse envelope
- ⁇ 2 represents group velocity dispersion
- T represents time
- ⁇ represents a nonlinear coefficient
- A+ represents the envelope of the right-handed circularly polarized soliton
- formula (2) is also applicable to the left-handed polarized soliton impulse (A+ is replaced by A-).
- the formula (2) can be converted into the energy of a circularly polarized soliton, and its formula is as follows:
- En+ represents the energy of the circularly polarized soliton
- En x represents the energy of the linearly polarized soliton
- the energy of the circularly polarized soliton is 1.56 times the energy of the linearly polarized soliton.
- the pump laser 11 used in the experiment is (FLCPA-02CSZU, Calmar), and its output laser is linearly polarized light with a wavelength of 1550 nm, pulse The width is 500fs and the repetition frequency is 1MHz.
- the energy ratio of the circularly polarized soliton to the linearly polarized soliton after passing through the long-pass filter 16 is about 1.56, which is consistent with the theoretical value of 1.5.
- the following experiments prove that the linearly polarized light generated by the pump laser 11 is converted into circularly polarized light after passing through the first quarter wave plate 12.
- a polarizer and a power meter are arranged behind the first quarter wave plate 12, the polarizer is rotated, and the power after the polarizer is rotated is measured by the power meter.
- the power of each angle of circularly polarized light is equal; however, in actual situations, the power of each angle of circularly polarized light will have a certain error.
- the abscissa is the angle and the ordinate is the normalized power, where the normalized power means that the power of each angle divided by the maximum power is the normalized power of the angle, such as ,
- the normalized power means that the power of each angle divided by the maximum power is the normalized power of the angle, such as .
- Now ten power values are measured at 0-360 degrees, and the power measured at 60 degrees is 23.5mw. Among them, the maximum power is 24.5mw, and the normalized power at 60 degrees is 23.5/24.5.
- the extinction ratio of circularly polarized light is 1, but in actual situations, the extinction ratio of circularly polarized light is close to 1, where the extinction ratio represents the ratio of the minimum power to the maximum power of circularly polarized light at different angles.
- the measured extinction ratio ERpump 1.06. It can be seen that the linearly polarized light generated by the pump laser 11 is converted into circularly polarized light after passing through the first quarter wave plate 12.
- the circularly polarized light becomes a circularly polarized soliton after passing through the optical fiber 14.
- a polarizer and a power meter are arranged behind the first collimating lens 15, the polarizer is rotated, and the polarizer is measured by the power meter. Power.
- the abscissa is the angle and the ordinate is the normalized power.
- the measured extinction ratio ERpump 1.03. It can be seen that the circularly polarized light passes through the optical fiber 14 and becomes a circularly polarized soliton.
- the circularly polarized soliton generator 1 further includes a first collimating lens 15 and a long-wave pass filter 16.
- the circularly polarized soliton is scattered after passing through the collimating lens, and the scattered circularly polarized soliton passes through the long wave
- the filter 16 is used for filtering.
- the long-pass filter 16 can filter circularly polarized solitons other than a specific wavelength. In this embodiment, the wavelength of the circularly polarized soliton allowed by the long-wavelength pass filter 16 is 1617 nm.
- the optical fiber 14 is a rod-shaped photonic crystal fiber. It can be understood that, in an alternative embodiment, the optical fiber 14 may also be a high-order mode optical fiber, a large mode field optical fiber, or a hollow core optical fiber.
- Fig. 4 is a schematic diagram of a multiphoton microscopy imaging system according to an embodiment of the present invention.
- the multiphoton microscopy imaging system 10 can have a second quarter wave plate 2, a flat field focusing lens 3, a dichroic mirror 4, an objective lens 5, a photomultiplier tube 6 and claim 1.
- the lens 3 and the dichroic mirror 4 are coupled into the objective lens 5, and are focused on the object to be detected through the objective lens 5 to generate a signal. After the signal is scattered, it passes through the objective lens 5 and the dichroic mirror 4 in turn.
- the dichroic mirror 4 transmits the signal It is coupled to the photomultiplier tube 6 and forms images through the photomultiplier tube 6.
- the multiphoton microscopy imaging system 10 further includes a first mirror 7, and the first mirror 7 is located between the second quarter wave plate 2 and the flat field focusing lens 3.
- the multiphoton microscopic imaging system 10 further includes a tube lens 8, and the tube lens 8 is located between the plan focusing lens 3 and the dichroic mirror 4.
- the multiphoton microscopic imaging system 10 further includes a second mirror 9 located between the tube lens 8 and the dichroic mirror 4.
- the dichroic mirror 4 can transmit long-wavelength optical solitons and reflect short-wavelength optical solitons.
- the dichroic mirror 4 can transmit an optical soliton with a wavelength of 1617 nm and reflect an optical soliton with a wavelength of 716 nm.
- the flat field focusing lens 3 includes an X-axis and Y-axis scanning mirror 31 and a scanning lens 32.
- the linearly polarized light generated by the pump laser 11 passes through the first quarter-wave plate and then becomes circularly polarized.
- the circularly polarized light passes through the focusing lens and is coupled into the rod-shaped photonic crystal fiber 14, and the circularly polarized light passes through the rod-shaped photonic crystal fiber 14 to become circularly polarized.
- the soliton, the circularly polarized soliton is diffused after passing through the collimating lens, the diffused circularly polarized soliton is filtered by the long-pass filter 16, and the filtered circularly polarized soliton is transformed into a linearly polarized soliton through the second quarter wave plate 2 ,
- the linearly polarized soliton is reflected by the first reflecting mirror 7, the reflected linearly polarized soliton is diffused after passing through the flat field focusing lens 3 and the tube lens 8, and the diffused linearly polarized soliton is reflected by the second reflecting mirror 9 and emitted
- the linearly polarized soliton is coupled to the objective lens 5 through the dichroic mirror 4, and the linearly polarized soliton is focused on the fluorescent dye of the test object through the objective lens 5, and the fluorescent dye produces a nonlinear effect (the fluorescent molecule absorbs three photons and jumps to the Excited state, the excited state changes to the ground state and emits a photon), thereby
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Abstract
A circularly polarized soliton generating device (1) and a multiphoton microscopic imaging system (10). The circularly polarized soliton generating device (1) comprises a pump laser (11), a first quarter-wave plate (12), a first focusing lens (13), and an optical fiber (14) arranged on a same optical path, where a linearly polarized light generated by the pump laser (11) is converted into a circularly polarized light via the first quarter-wave plate (12), the circularly polarized light is coupled into the optical fiber (14) via the first focusing lens (13), and the circularly polarized light is converted into a circularly polarized soliton via the optical fiber (14). The linearly polarized light is converted into the circularly polarize light via the first quarter-wave plate (12), and then the circularly polarized light is converted into the circularly polarized soliton via the optical fiber (14). Under same conditions, the energy of a circularly polarized soliton is 1.56 times of the energy of a linearly polarized soliton; this enables a multiphoton signal to be transmitted further in the brain, thus allowing humans to further investigate tissues deep in the brain, and unraveling the mystery of the brain.
Description
本申请要求于2019年06月26日在中华人民共和国国家知识产权局提交的、申请号为CN201910552925.X、发明名称为“圆偏振孤子产生装置及多光子显微成像***”的中国专利申请的优先权，其全部内容通过引用结合在本申请中。This application requires a Chinese patent application filed at the State Intellectual Property Office of the People’s Republic of China on June 26, 2019, with an application number of CN201910552925.X and an invention title of "Circularly Polarized Soliton Generator and Multiphoton Microscopic Imaging System". Priority, the entire content of which is incorporated in this application by reference.
本发明涉及光学成像技术领域，尤其涉及一种圆偏振孤子产生装置及多光子显微成像***。The invention relates to the technical field of optical imaging, in particular to a circularly polarized soliton generator and a multiphoton microscopic imaging system.
光孤子(Soliton，Solitons in optical fibres)是指经过长距离传输而保持形状不变的光脉冲。一束光脉冲包含许多不同的频率成分，频率不同，在介质中的传播速度也不同，因此，光脉冲在光纤中将发生色散，使得脉宽变宽。但当具有高强度的极窄单色光脉冲入射到光纤中时，将产生克尔效应，即介质的折射率随光强度而变化，由此导致在光脉冲中产生自相位调制，使脉冲前沿产生的相位变化引起频率降低，脉冲后沿产生的相位变化引起频率升高，于是脉冲前沿比其后沿传播得慢，从而使脉宽变窄。当脉冲具有适当的幅度时，以上两种作用可以恰好抵消，则脉冲可以保持波形稳定不变地在光纤中传输，即形成了光孤子，也称为基阶光孤子。若脉冲幅度继续增大时，变窄效应将超过变宽效应，则形成高阶光孤子，它在光纤中传输的脉冲形状将发生连续变化，首先压缩变窄，然后***，在特定距离处脉冲周期性地复原。Soliton (Solitons in optical fibres) refers to light pulses that are transmitted over a long distance while keeping their shape unchanged. A beam of light pulse contains many different frequency components, the frequency is different, and the propagation speed in the medium is also different. Therefore, the light pulse will be dispersed in the optical fiber, making the pulse width wider. However, when a very narrow monochromatic light pulse with high intensity is incident on the optical fiber, the Kerr effect will occur, that is, the refractive index of the medium changes with the light intensity, which leads to self-phase modulation in the light pulse, making the pulse front The resulting phase change causes the frequency to decrease, and the phase change produced by the trailing edge of the pulse causes the frequency to rise, so the leading edge of the pulse propagates slower than the trailing edge, thus narrowing the pulse width. When the pulse has an appropriate amplitude, the above two effects can be exactly offset, and the pulse can be transmitted in the fiber with a stable waveform, that is, an optical soliton is formed, which is also called a fundamental-order optical soliton. If the pulse amplitude continues to increase, the narrowing effect will exceed the widening effect, and a high-order optical soliton will be formed. The pulse shape transmitted in the fiber will change continuously. First, it will be compressed and narrowed, then split, and the pulse will be periodic at a certain distance. To restore.
光孤子是由光纤中两种最基本的物理现象，即群速度色散(GVD)和自相位调制(SPM)共同作用形成的。Optical soliton is formed by two basic physical phenomena in optical fiber, namely group velocity dispersion (GVD) and self-phase modulation (SPM).
光脉冲在光纤中传输时总是存在一定的频率范围，在线性近拟中，常将光脉冲表示成在一定范围内一系列简谐波的叠加。由于各谐波分量相速度不同，因而光脉冲包络的传输通常以群速vg＝dω/dβ来表示(β为光波波数，ω为载波频率)。由该式可见，群速度是随着频率的变化而变化的，而光脉冲中不同频率的分量则会以不同的速度进行传播，导致脉冲的分散，这种现象称之为群速度色散(GVD)。研究的结果表明，λd＝1310nm处为零色散波长，λ>λd称之为反常色散区域，λ<λd称之为正常色散区域。正常与反常色散区域光脉冲的传输特性是不同的，在反常色散区域，光脉冲的高频分量(蓝移)较低频分量(红移)传输得快，而在正常色散区域，情况正好相反。由于传输情况不同，群速度色散效应不同，最终导致了光脉冲的展宽。There is always a certain frequency range when light pulses are transmitted in optical fibers. In linear approximation, light pulses are often expressed as the superposition of a series of simple harmonics within a certain range. Since the phase velocity of each harmonic component is different, the transmission of the optical pulse envelope is usually represented by the group velocity vg=dω/dβ (β is the light wave number and ω is the carrier frequency). It can be seen from this formula that the group velocity changes with the frequency, and the components of different frequencies in the light pulse will propagate at different speeds, resulting in the dispersion of the pulse. This phenomenon is called group velocity dispersion (GVD ). The results of the study show that λd=1310nm is the zero-dispersion wavelength, λ>λd is called the abnormal dispersion region, and λ<λd is called the normal dispersion region. The transmission characteristics of light pulses in the normal and abnormal dispersion regions are different. In the abnormal dispersion region, the high frequency components (blue shift) and lower frequency components (red shift) of the light pulse are transmitted faster, while in the normal dispersion region, the situation is just the opposite. . Due to different transmission conditions, the group velocity dispersion effect is different, which ultimately leads to the broadening of the optical pulse.
自相位调制效应是光波在光纤中传输时光波本身引起的相移。其起源于光纤的折射率n与电场强度I之间的非线性效应—克尔(kerr)效应，即：n＝n0+n2I。上式中，n＝1.45是线性折射率，n2＝6.1×1023V/m为非线性折射率系数。由上式可知，不同强度的脉冲分量相速度是不同的，这样，在光脉冲传输的过程中将会产生不同的相移，结果会造成脉冲谱的变化。例如，通过对于高斯脉冲的分析表明，自相位调制会导致脉冲前沿谱红移，后沿谱蓝移，对其它形状脉冲的分析也有类似的结果。另外，相对在群速度色散(GVD)的反常色散区，脉冲的高频(蓝移)分量运动速度要高于低频(红移)分量，而自相位调制(SPM)效应所导致的脉冲前沿谱红移又使脉冲前沿运动速度减慢和脉冲后沿由于谱蓝移而加快运动速度，进而使得脉冲变窄，正好与群速度色散在反常色散区的脉冲展宽的趋势相对应。因此，当这两种作用在数量上达到平衡时，光脉冲就会保持不变而成为光孤粒子，即光孤子。所以说，光孤子的形成机理是光纤中群速度色散和自相位调制效应在反常色散区的精确平衡。The self-phase modulation effect is the phase shift caused by the light wave itself when the light wave propagates in the optical fiber. It originates from the non-linear effect between the refractive index n of the optical fiber and the electric field intensity I-the Kerr effect, namely: n=n0+n2I. In the above formula, n=1.45 is the linear refractive index, and n2=6.1×1023V/m is the nonlinear refractive index. It can be seen from the above formula that the phase velocities of pulse components of different intensities are different. In this way, different phase shifts will be produced during the transmission of the optical pulses, which will result in changes in the pulse spectrum. For example, the analysis of Gaussian pulses shows that self-phase modulation will cause a red shift in the leading edge of the pulse and a blue shift in the trailing edge. The analysis of pulses of other shapes has similar results. In addition, relative to the anomalous dispersion region of group velocity dispersion (GVD), the high-frequency (blueshift) component of the pulse moves faster than the low-frequency (redshift) component, and the leading edge spectrum of the pulse caused by the self-phase modulation (SPM) effect Red shift slows down the movement of the leading edge of the pulse and speeds up the movement of the trailing edge of the pulse due to spectral blue shift, which makes the pulse narrower, which corresponds to the trend of pulse broadening in the anomalous dispersion region with group velocity dispersion. Therefore, when these two effects reach a balance in quantity, the light pulse will remain unchanged and become an optical soliton, that is, an optical soliton. Therefore, the formation mechanism of optical soliton is the precise balance of group velocity dispersion and self-phase modulation effect in the anomalous dispersion region in the fiber.
1986年，Mitschke和Mollenauer发现光纤中的孤子自频移(SSFS)的非线性光学效应：当超短光孤子在反常色散光纤内传播时，会经历连续波长向长波长转移。由孤子自频移技术能够产生波长可调谐的光孤子，该光孤子具有以下特点：超短的脉冲宽度(几十飞秒到亚皮秒量级)、优异的脉冲质量以及宽带波长的可调谐性。这些特点使其成为多光子显微成像技术(MPM)极为理想的光源选择。In 1986, Mitschke and Mollenauer discovered the nonlinear optical effect of the soliton self-frequency shift (SSFS) in the optical fiber: when the ultrashort optical soliton propagates in the anomalous dispersion fiber, it will experience a continuous wavelength shift to a long wavelength. The self-frequency shift technology of soliton can generate optical soliton with tunable wavelength. The optical soliton has the following characteristics: ultra-short pulse width (tens of femtoseconds to sub-picosecond order), excellent pulse quality and tunable broadband wavelength Sex. These characteristics make it an ideal light source choice for multiphoton microscopy (MPM).
多光子显微成像是一种非线性光学成像技术，特别适用于活体深层组织成像。多光子显微成像技术以不同的模态在生物学、生理学以及医学研究中得到了广泛的应用。Multiphoton microscopy imaging is a nonlinear optical imaging technique, especially suitable for deep tissue imaging in living body. Multiphoton microscopy imaging technology has been widely used in biology, physiology and medical research in different modalities.
多光子信号在大脑中受到光孤子能量的限制，使得多光子信号在脑组织传播的过程中，随着脑组织的吸收和散射呈指数衰减趋势。在相关技术中，由于多光子显微成像***产生的光孤子能量较低，导致现有的多光子显微成像***只能研究大脑浅层的组织，因此，无法进一步研究大脑深层的组织，从而限制了人类对大脑深层的进一步探索。The multiphoton signal is limited by the energy of the optical soliton in the brain, so that the multiphoton signal will exponentially decay with the absorption and scattering of the brain tissue during the propagation of the multiphoton signal in the brain tissue. In related technologies, due to the low energy of the optical soliton generated by the multiphoton microscopy imaging system, the existing multiphoton microscopy imaging system can only study the shallow tissues of the brain. Therefore, it is impossible to further study the deep tissues of the brain. It limits the further exploration of the deep layers of the brain.
本发明的主要目的在于提供一种圆偏振孤子产生装置及多光子显微成像***，旨在解决现有技术中产生的光孤子能量较低的技术问题。The main purpose of the present invention is to provide a circularly polarized soliton generating device and a multiphoton microscopic imaging system, aiming to solve the technical problem of low energy of the optical soliton generated in the prior art.
为解决上述技术问题，本申请实施例采用的技术方案是：In order to solve the above technical problems, the technical solutions adopted in the embodiments of this application are:
一种圆偏振孤子产生装置，包括位于同一光路上的泵浦激光器、第一四分之一波片、第一聚焦透镜以及光纤，其中，所述泵浦激光器产生的线偏光经过第一四分之一波片后转变为圆偏光，圆偏光经过所述聚焦透镜后耦合到所述光纤内，圆偏光经过所述光纤后转变为圆偏振孤子。A circularly polarized soliton generator includes a pump laser, a first quarter wave plate, a first focusing lens, and an optical fiber on the same optical path, wherein the linearly polarized light generated by the pump laser passes through the first quarter One of the wave plates is transformed into circularly polarized light, the circularly polarized light is coupled into the optical fiber after passing through the focusing lens, and the circularly polarized light is transformed into a circularly polarized soliton after passing through the optical fiber.
其中，所述圆偏振孤子产生装置还包括第一准直透镜和长波通滤光片，其中，圆偏振孤子经过所述准直透镜后发生散射，散射后的圆偏振孤子经过所述长波通滤光片进行过滤。Wherein, the circularly polarized soliton generating device further includes a first collimating lens and a long-pass filter, wherein the circularly polarized soliton is scattered after passing through the collimating lens, and the scattered circularly polarized soliton passes through the long-pass filter. The light sheet is filtered.
其中，所述光纤为棒状光子晶体光纤。Wherein, the optical fiber is a rod-shaped photonic crystal optical fiber.
一种多光子显微成像***，包括第二四分之一波片、平场聚焦透镜、二向色镜、物镜、光电倍增管以及权利要求1-3任一项所述的圆偏振孤子产生装置；其中，所述圆偏振孤子产生装置产生的圆偏振孤子经过所述第二四分之一波片后转变为线偏振孤子，线偏振孤子经过所述平场聚焦透镜和所述二向色镜后耦合到物镜内，并通过物镜聚焦到待检测物上产生信号，信号发生散射后依次经过所述物镜和所述二向色镜，所述二向色镜将信号耦合到所述光电倍增管内，并且通过所述光电倍增管成像。A multiphoton microscopic imaging system, comprising a second quarter wave plate, a flat field focusing lens, a dichroic mirror, an objective lens, a photomultiplier tube, and the circularly polarized soliton generator according to any one of claims 1-3 Device; wherein, the circularly polarized soliton generated by the circularly polarized soliton generator is transformed into a linearly polarized soliton after passing through the second quarter wave plate, and the linearly polarized soliton passes through the flat field focusing lens and the dichroic The rear lens is coupled to the objective lens, and the signal is generated by focusing on the object to be detected through the objective lens. The signal is scattered and then passes through the objective lens and the dichroic mirror, and the dichroic mirror couples the signal to the photomultiplier Inside the tube and imaged through the photomultiplier tube.
其中，所述多光子显微成像***还包括第一反射镜，所述第一反射镜位于所述第二四分之一波片和所述平场聚焦透镜之间。Wherein, the multiphoton microscopic imaging system further includes a first reflector, and the first reflector is located between the second quarter wave plate and the plan focusing lens.
其中，所述多光子显微成像***还包括镜筒透镜，所述镜筒透镜位于所述平场聚焦透镜和所述二向色镜之间。Wherein, the multiphoton microscopic imaging system further includes a tube lens, and the tube lens is located between the plan focusing lens and the dichroic mirror.
其中，所述多光子显微成像***还包括第二反射镜，所述第二反射镜位于所述镜筒透镜和所述二向色镜之间。Wherein, the multiphoton microscopy imaging system further includes a second reflector, and the second reflector is located between the tube lens and the dichroic mirror.
上述圆偏振孤子产生装置及多光子显微成像***，通过第一四分之一波片，使线偏光变为圆偏光，再将圆偏光经过光纤后变成圆偏振孤子。经过试验证明，在同等条件下，圆偏振孤子的能量是线偏振孤子能量的1.56倍，使得多光子信号能够在大脑中传输的更远，从而使人类能够进一步探索大脑深层的组织，进而解开大脑神秘的面纱。The above-mentioned circularly polarized soliton generator and multiphoton microscopic imaging system pass the first quarter-wave plate to convert linearly polarized light into circularly polarized light, and then turn the circularly polarized light into circularly polarized soliton after passing through an optical fiber. Experiments have shown that under the same conditions, the energy of the circularly polarized soliton is 1.56 times the energy of the linearly polarized soliton, which enables multiphoton signals to be transmitted farther in the brain, so that humans can further explore the deep tissues of the brain and untie it. The mysterious veil of the brain.
为了更清楚地说明本申请实施例中的技术方案，下面将对实施例或示范性技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本申请的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其它的附图。In order to more clearly describe the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings that need to be used in the embodiments or exemplary technical descriptions. Obviously, the drawings in the following description are only for the present application. For some embodiments, those of ordinary skill in the art can obtain other drawings based on these drawings without creative work.
图1是根据本发明的一个实施例的圆偏振孤子产生装置的示意图。Fig. 1 is a schematic diagram of a circularly polarized soliton generator according to an embodiment of the present invention.
图2是根据本发明的一个实施例的圆偏振孤子和线偏振孤子经过长波通滤光片的能量对比图。Fig. 2 is a comparison diagram of the energy of a circularly polarized soliton and a linearly polarized soliton passing through a long-pass filter according to an embodiment of the present invention.
图3(a)是根据本发明的一个实施例的线偏光经过第一四分之一波片的归一化泵浦功率和偏振片旋转角度的关系图。Fig. 3(a) is a graph of the relationship between the normalized pump power of the linearly polarized light passing through the first quarter wave plate and the rotation angle of the polarizer according to an embodiment of the present invention.
图3(b)是根据本发明的一个实施例的圆偏光经过准直透镜的归一化泵浦功率和偏振片旋转角度的关系图。Fig. 3(b) is a diagram of the relationship between the normalized pump power of the circularly polarized light passing through the collimator lens and the rotation angle of the polarizer according to an embodiment of the present invention.
图4是根据本发明的一个实施例的多光子显微成像***的示意图。Fig. 4 is a schematic diagram of a multiphoton microscopy imaging system according to an embodiment of the present invention.
10、多光子显微成像***；1、圆偏振孤子产生装置；11、泵浦激光器；12、第一四分之一波片；13、第一聚焦透镜；14、光纤；15、第一准直透镜；16、长波通滤光片；2、第二四分之一波片；3、平场聚焦透镜；31、扫描镜；32、扫描透镜；4、二向色镜；5、物镜；6、光电倍增管；7、第一反射镜；8、镜筒透镜；9、第二反射镜。10. Multiphoton microscopy imaging system; 1. Circular polarization soliton generator; 11. Pump laser; 12. First quarter wave plate; 13. First focusing lens; 14. Optical fiber; 15. First alignment Straight lens; 16. Long wave pass filter; 2. Second quarter wave plate; 3. Plan focusing lens; 31. Scanning mirror; 32. Scanning lens; 4. Dichroic mirror; 5. Objective; 6. Photomultiplier tube; 7. First reflector; 8. Tube lens; 9. Second reflector.
为使得本发明的发明目的、特征、优点能够更加的明显和易懂，下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而非全部实施例。基于本发明中的实施例，本领域技术人员在没有做出创造性劳动前提下所获得的 所有其他实施例，都属于本发明保护的范围。In order to make the objectives, features, and advantages of the present invention more obvious and understandable, the technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the description The embodiments are only a part of the embodiments of the present invention, but not all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work shall fall within the protection scope of the present invention.
图1是根据本发明的一个实施例的圆偏振孤子产生装置的示意图。Fig. 1 is a schematic diagram of a circularly polarized soliton generator according to an embodiment of the present invention.
从图中可以看出，该圆偏振孤子产生装置1可以具有位于同一光路上的泵浦激光器11、第一四分之一波片12、第一聚焦透镜13以及光纤14，其中，泵浦激光器11产生的线偏光经过第一四分之一波片12后转变为圆偏光，圆偏光经过聚焦透镜后耦合到光纤14内，圆偏光经过光纤14后转变为圆偏振孤子。It can be seen from the figure that the circularly polarized soliton generator 1 may have a pump laser 11, a first quarter wave plate 12, a first focusing lens 13, and an optical fiber 14 on the same optical path, wherein the pump laser The linearly polarized light generated by 11 is transformed into circularly polarized light after passing through the first quarter wave plate 12, and the circularly polarized light is coupled into the optical fiber 14 after passing through the focusing lens, and the circularly polarized light is transformed into circularly polarized soliton after passing through the optical fiber 14.
在本实施例中，通过第一四分之一波片12，使线偏光变为圆偏光，再将圆偏光经过光纤14后变成圆偏振孤子。经过试验证明，在同等条件下，圆偏振孤子的能量是线偏振孤子能量的1.56倍，使得多光子信号能够在大脑中传输的更远，从而使人类能够进一步探索大脑深层的组织，进而解开大脑神秘的面纱。In this embodiment, the linearly polarized light is changed to circularly polarized light through the first quarter wave plate 12, and then the circularly polarized light passes through the optical fiber 14 and becomes a circularly polarized soliton. Experiments have shown that under the same conditions, the energy of the circularly polarized soliton is 1.56 times the energy of the linearly polarized soliton, which enables multiphoton signals to be transmitted farther in the brain, so that humans can further explore the deep tissues of the brain and untie it. The mysterious veil of the brain.
以下通过理论证明圆偏振孤子的能量是线偏振孤子能量的1.56倍，线偏振孤子在光纤14中的传播公式为：The following theoretically proves that the energy of the circularly polarized soliton is 1.56 times the energy of the linearly polarized soliton, and the propagation formula of the linearly polarized soliton in the fiber 14 is:
其中，i表示虚数单位，表示微分符号，z表示传输距离，Ax表示线偏振脉冲包络，β2表示群速度色散，T表示时间，γ表示非线性系数。Among them, i represents an imaginary unit, representing a differential symbol, z represents a transmission distance, Ax represents a linearly polarized pulse envelope, β2 represents group velocity dispersion, T represents time, and γ represents a nonlinear coefficient.
圆偏振孤子在光纤14中的传播公式为：The propagation formula of circularly polarized soliton in fiber 14 is:
其中，A+表示右旋圆偏振孤子包络，公式(2)对左旋偏孤子冲同样适用(A+换为A-)。Among them, A+ represents the envelope of the right-handed circularly polarized soliton, and formula (2) is also applicable to the left-handed polarized soliton impulse (A+ is replaced by A-).
由公式(1)和公式(2)可定义一个新的非线性系数γ’＝2/3γ，使得二者一致。公式(2)可转换为圆偏振孤子的能量，其公式如下：A new nonlinear coefficient γ'=2/3γ can be defined by formula (1) and formula (2), so that the two are consistent. The formula (2) can be converted into the energy of a circularly polarized soliton, and its formula is as follows:
其中，En+表示圆偏振孤子的能量，En x表示线偏振孤子的能量。Among them, En+ represents the energy of the circularly polarized soliton, and En x represents the energy of the linearly polarized soliton.
由公式(3)可知，圆偏振孤子的能量是线偏振孤子能量的1.5倍。It can be seen from formula (3) that the energy of the circularly polarized soliton is 1.5 times the energy of the linearly polarized soliton.
以下通过实验证明圆偏振孤子的能量是线偏振孤子能量的1.56倍，在本实施例中，实验所用泵浦激光器11为(FLCPA-02CSZU,Calmar)，其输出激光为波长1550nm的线偏光，脉冲宽度为500fs，重复频率为1MHz。如图2中所示，在同样的条件下，圆偏振孤子与线偏振孤子经过长波通滤光片16后的能量比为1.56左右，与理论值1.5相吻合。The following experiments prove that the energy of the circularly polarized soliton is 1.56 times the energy of the linearly polarized soliton. In this embodiment, the pump laser 11 used in the experiment is (FLCPA-02CSZU, Calmar), and its output laser is linearly polarized light with a wavelength of 1550 nm, pulse The width is 500fs and the repetition frequency is 1MHz. As shown in FIG. 2, under the same conditions, the energy ratio of the circularly polarized soliton to the linearly polarized soliton after passing through the long-pass filter 16 is about 1.56, which is consistent with the theoretical value of 1.5.
以下通过实验证明泵浦激光器11产生的线偏光经过第一四分之一波片12后转变为圆偏光。在第一四分之一波片12后面设置偏振片和功率计，旋转偏振片，并通过功率计测量偏振片旋转后的功率。在理论情况下，圆偏光每个角度的功率都相等；但是，在实际情况下，圆偏光每个角度的功率会存在一定的误差。如图3(a)中所示，横坐标为角度，纵坐标为归一化功率，其中，归一化功率表示将每个角度的功率除以最大功率就是该角度的归一化功率，比如，现在在0-360度测量十个功率值，在60度测量的功率为23.5mw,其中，最大的功率为24.5mw，则60度的归一化功率为23.5/24.5。在理论情况下，圆偏光的消光比为1，但是，在实际情况下，圆偏光的消光比接近1，其中，消光比表示圆偏光在不同角度下的最小功率和最大功率之比。最终，试验测得的消光比ERpump＝1.06，由此可知，泵浦激光器11产生的线偏光经过第一四分之一波片12后转变为圆偏光。The following experiments prove that the linearly polarized light generated by the pump laser 11 is converted into circularly polarized light after passing through the first quarter wave plate 12. A polarizer and a power meter are arranged behind the first quarter wave plate 12, the polarizer is rotated, and the power after the polarizer is rotated is measured by the power meter. In theory, the power of each angle of circularly polarized light is equal; however, in actual situations, the power of each angle of circularly polarized light will have a certain error. As shown in Figure 3(a), the abscissa is the angle and the ordinate is the normalized power, where the normalized power means that the power of each angle divided by the maximum power is the normalized power of the angle, such as , Now ten power values are measured at 0-360 degrees, and the power measured at 60 degrees is 23.5mw. Among them, the maximum power is 24.5mw, and the normalized power at 60 degrees is 23.5/24.5. In theory, the extinction ratio of circularly polarized light is 1, but in actual situations, the extinction ratio of circularly polarized light is close to 1, where the extinction ratio represents the ratio of the minimum power to the maximum power of circularly polarized light at different angles. Finally, the measured extinction ratio ERpump=1.06. It can be seen that the linearly polarized light generated by the pump laser 11 is converted into circularly polarized light after passing through the first quarter wave plate 12.
以下通过实验证明圆偏光经过光纤14后变成圆偏振孤子，在本实施例中，在第一准直透镜15后面设置偏振片和功率计，旋转偏振片，并通过功率计测 量偏振片旋转后的功率。如图3(b)中所示，横坐标为角度，纵坐标为归一化功率。最终，试验测得的消光比ERpump＝1.03，由此可知，圆偏光经过光纤14后变成圆偏振孤子。The following experiment proves that the circularly polarized light becomes a circularly polarized soliton after passing through the optical fiber 14. In this embodiment, a polarizer and a power meter are arranged behind the first collimating lens 15, the polarizer is rotated, and the polarizer is measured by the power meter. Power. As shown in Figure 3(b), the abscissa is the angle and the ordinate is the normalized power. Finally, the measured extinction ratio ERpump=1.03. It can be seen that the circularly polarized light passes through the optical fiber 14 and becomes a circularly polarized soliton.
在图示实施例中，圆偏振孤子产生装置1还包括第一准直透镜15和长波通滤光片16，其中，圆偏振孤子经过准直透镜后发生散射，散射后的圆偏振孤子经过长波通滤光片16进行过滤。通过长波通滤光片16能够过滤特定波长以外的圆偏振孤子。在本实施例中，长波通滤光片16允许通过的圆偏振孤子的波长为1617nm。In the illustrated embodiment, the circularly polarized soliton generator 1 further includes a first collimating lens 15 and a long-wave pass filter 16. The circularly polarized soliton is scattered after passing through the collimating lens, and the scattered circularly polarized soliton passes through the long wave The filter 16 is used for filtering. The long-pass filter 16 can filter circularly polarized solitons other than a specific wavelength. In this embodiment, the wavelength of the circularly polarized soliton allowed by the long-wavelength pass filter 16 is 1617 nm.
在本实施例中，光纤14为棒状光子晶体光纤。可以了解，在可选的实施例中，光纤14也可以为高阶模光纤、大模场光纤或者空芯光纤。In this embodiment, the optical fiber 14 is a rod-shaped photonic crystal fiber. It can be understood that, in an alternative embodiment, the optical fiber 14 may also be a high-order mode optical fiber, a large mode field optical fiber, or a hollow core optical fiber.
图4是根据本发明的一个实施例的多光子显微成像***的示意图。Fig. 4 is a schematic diagram of a multiphoton microscopy imaging system according to an embodiment of the present invention.
从图中可以看出，该多光子显微成像***10可以具有第二四分之一波片2、平场聚焦透镜3、二向色镜4、物镜5、光电倍增管6以及权利要求1-3任一项的圆偏振孤子产生装置1；其中，圆偏振孤子产生装置1产生的圆偏振孤子经过第二四分之一波片2后转变为线偏振孤子，线偏振孤子经过平场聚焦透镜3和二向色镜4后耦合到物镜5内，并通过物镜5聚焦到待检测物上产生信号，信号发生散射后依次经过物镜5和二向色镜4，二向色镜4将信号耦合到光电倍增管6内，并且通过光电倍增管6成像。It can be seen from the figure that the multiphoton microscopy imaging system 10 can have a second quarter wave plate 2, a flat field focusing lens 3, a dichroic mirror 4, an objective lens 5, a photomultiplier tube 6 and claim 1. -3 The circularly polarized soliton generator 1 of any item; wherein the circularly polarized soliton generated by the circularly polarized soliton generator 1 is transformed into a linearly polarized soliton after passing through the second quarter-wave plate 2, and the linearly polarized soliton is focused by a flat field The lens 3 and the dichroic mirror 4 are coupled into the objective lens 5, and are focused on the object to be detected through the objective lens 5 to generate a signal. After the signal is scattered, it passes through the objective lens 5 and the dichroic mirror 4 in turn. The dichroic mirror 4 transmits the signal It is coupled to the photomultiplier tube 6 and forms images through the photomultiplier tube 6.
在图示实施例中，多光子显微成像***10还包括第一反射镜7，第一反射镜7位于第二四分之一波片2和平场聚焦透镜3之间。In the illustrated embodiment, the multiphoton microscopy imaging system 10 further includes a first mirror 7, and the first mirror 7 is located between the second quarter wave plate 2 and the flat field focusing lens 3.
在图示实施例中，多光子显微成像***10还包括镜筒透镜8，镜筒透镜8位于平场聚焦透镜3和二向色镜4之间。In the illustrated embodiment, the multiphoton microscopic imaging system 10 further includes a tube lens 8, and the tube lens 8 is located between the plan focusing lens 3 and the dichroic mirror 4.
在图示实施例中，多光子显微成像***10还包括第二反射镜9，第二反 射镜9位于镜筒透镜8和二向色镜4之间。二向色镜4能够透射长波长的光孤子，反射短波长的光孤子。在本实施例中，二向色镜4能够透射1617nm波长的光孤子，反射716nm波长的光孤子。In the illustrated embodiment, the multiphoton microscopic imaging system 10 further includes a second mirror 9 located between the tube lens 8 and the dichroic mirror 4. The dichroic mirror 4 can transmit long-wavelength optical solitons and reflect short-wavelength optical solitons. In this embodiment, the dichroic mirror 4 can transmit an optical soliton with a wavelength of 1617 nm and reflect an optical soliton with a wavelength of 716 nm.
在本实施例中，平场聚焦透镜3包括X、Y轴扫描镜31和扫描透镜32。In this embodiment, the flat field focusing lens 3 includes an X-axis and Y-axis scanning mirror 31 and a scanning lens 32.
工作过程：work process:
泵浦激光器11产生的线偏光经过第一四分之波片后变为圆偏光，圆偏光经过聚焦透镜后耦合到棒状光子晶体光纤14内，圆偏光经过棒状光子晶体光纤14后变为圆偏振孤子，圆偏振孤子经过准直透镜后发生扩散，扩散后的圆偏振孤子经过长波通滤光片16进行过滤，过滤后的圆偏振孤子经过第二四分之一波片2变为线偏振孤子，线偏振孤子经过第一反射镜7发生反射，反射后的线偏振孤子经过平场聚焦透镜3和镜筒透镜8后发生扩散，扩散后的线偏振孤子经过第二反射镜9发生反射，发射后的线偏振孤子经过二向色镜4耦合到物镜5内，线偏振孤子经过物镜5聚焦到待检物的荧光染料上，并且使荧光染料产生非线性效应(荧光分子吸收三个光子跃进到激发态，激发态变为基态并发射一个光子)，从而产生荧光信号，荧光信号发生散射后依次经过物镜5和二向色镜4，二向色镜4将荧光信号耦合到光电倍增管6内，最终，通过光电倍增管6成像。The linearly polarized light generated by the pump laser 11 passes through the first quarter-wave plate and then becomes circularly polarized. The circularly polarized light passes through the focusing lens and is coupled into the rod-shaped photonic crystal fiber 14, and the circularly polarized light passes through the rod-shaped photonic crystal fiber 14 to become circularly polarized. The soliton, the circularly polarized soliton is diffused after passing through the collimating lens, the diffused circularly polarized soliton is filtered by the long-pass filter 16, and the filtered circularly polarized soliton is transformed into a linearly polarized soliton through the second quarter wave plate 2 , The linearly polarized soliton is reflected by the first reflecting mirror 7, the reflected linearly polarized soliton is diffused after passing through the flat field focusing lens 3 and the tube lens 8, and the diffused linearly polarized soliton is reflected by the second reflecting mirror 9 and emitted The linearly polarized soliton is coupled to the objective lens 5 through the dichroic mirror 4, and the linearly polarized soliton is focused on the fluorescent dye of the test object through the objective lens 5, and the fluorescent dye produces a nonlinear effect (the fluorescent molecule absorbs three photons and jumps to the Excited state, the excited state changes to the ground state and emits a photon), thereby generating a fluorescent signal. After the fluorescent signal is scattered, it passes through the objective lens 5 and the dichroic mirror 4, and the dichroic mirror 4 couples the fluorescent signal into the photomultiplier tube 6. , Finally, through the photomultiplier tube 6 imaging.
以上为对本发明所提供的一种圆偏振孤子产生装置及多光子显微成像***的描述，对于本领域的技术人员，依据本发明实施例的思想，在具体实施例及应用范围上均会有改变之处，综上，本说明书内容不应理解为对本发明的限制。The above is a description of a circularly polarized soliton generator and a multiphoton microscopy imaging system provided by the present invention. For those skilled in the art, according to the ideas of the embodiments of the present invention, there will be specific embodiments and application scopes. In summary, the content of this specification should not be construed as limiting the present invention.
Claims (7)
- 一种圆偏振孤子产生装置，其特征在于，包括位于同一光路上的泵浦激光器、第一四分之一波片、第一聚焦透镜以及光纤，其中，所述泵浦激光器产生的线偏光经过第一四分之一波片后转变为圆偏光，圆偏光经过所述聚焦透镜后耦合到所述光纤内，圆偏光经过所述光纤后转变为圆偏振孤子。A circularly polarized soliton generator is characterized in that it comprises a pump laser, a first quarter wave plate, a first focusing lens and an optical fiber located on the same optical path, wherein the linearly polarized light generated by the pump laser passes through The first quarter wave plate is converted into circularly polarized light, the circularly polarized light is coupled into the optical fiber after passing through the focusing lens, and the circularly polarized light is converted into a circularly polarized soliton after passing through the optical fiber.
- 根据权利要求1所述的圆偏振孤子产生装置，其特征在于，所述圆偏振孤子产生装置还包括第一准直透镜和长波通滤光片，其中，圆偏振孤子经过所述准直透镜后发生散射，散射后的圆偏振孤子经过所述长波通滤光片进行过滤。The circularly polarized soliton generator according to claim 1, wherein the circularly polarized soliton generator further comprises a first collimating lens and a long-wave pass filter, wherein the circularly polarized soliton passes through the collimator lens. Scattering occurs, and the scattered circularly polarized soliton is filtered by the long-wave pass filter.
- 根据权利要求1所述的圆偏振孤子产生装置，其特征在于，所述光纤为棒状光子晶体光纤。The circularly polarized soliton generator according to claim 1, wherein the optical fiber is a rod-shaped photonic crystal fiber.
- 一种多光子显微成像***，其特征在于，包括第二四分之一波片、平场聚焦透镜、二向色镜、物镜、光电倍增管以及权利要求1-3任一项所述的圆偏振孤子产生装置；其中，所述圆偏振孤子产生装置产生的圆偏振孤子经过所述第二四分之一波片后转变为线偏振孤子，线偏振孤子经过所述平场聚焦透镜和二向色镜后耦合到物镜内，并通过物镜聚焦到待检测物上产生信号，信号发生散射后依次经过所述物镜和所述二向色镜，所述二向色镜将信号耦合到所述光电倍增管内，并且通过所述光电倍增管成像。A multiphoton microscopy imaging system, which is characterized by comprising a second quarter wave plate, a flat field focusing lens, a dichroic lens, an objective lens, a photomultiplier tube, and a photomultiplier tube according to any one of claims 1 to 3 A circularly polarized soliton generator; wherein the circularly polarized soliton generated by the circularly polarized soliton generator is converted into a linearly polarized soliton after passing through the second quarter wave plate, and the linearly polarized soliton passes through the flat field focusing lens and two The dichroic mirror is coupled to the objective lens, and is focused on the object to be detected through the objective lens to generate a signal. The signal is scattered and then passes through the objective lens and the dichroic mirror. The dichroic mirror couples the signal to the Inside a photomultiplier tube, and imaging through the photomultiplier tube.
- 根据权利要求4所述的多光子显微成像***，其特征在于，所述多光子显微成像***还包括第一反射镜，所述第一反射镜位于所述第二四分之一波片 和所述平场聚焦透镜之间。The multiphoton microscopy imaging system of claim 4, wherein the multiphoton microscopy imaging system further comprises a first reflector, and the first reflector is located on the second quarter wave plate And the flat-field focusing lens.
- 根据权利要求4所述的多光子显微成像***，其特征在于，所述多光子显微成像***还包括镜筒透镜，所述镜筒透镜位于所述平场聚焦透镜和所述二向色镜之间。The multiphoton microscopy imaging system according to claim 4, wherein the multiphoton microscopy imaging system further comprises a tube lens, and the tube lens is located between the plan focusing lens and the dichroic lens. Between the mirrors.
- 根据权利要求6所述的多光子显微成像***，其特征在于，所述多光子显微成像***还包括第二反射镜，所述第二反射镜位于所述镜筒透镜和所述二向色镜之间。The multiphoton microscopy imaging system according to claim 6, wherein the multiphoton microscopy imaging system further comprises a second mirror, and the second mirror is located between the tube lens and the two-way mirror. Between color mirrors.
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