CN106532426B - A kind of enhancement device of multi-photon imaging signal - Google Patents
A kind of enhancement device of multi-photon imaging signal Download PDFInfo
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- CN106532426B CN106532426B CN201710013169.4A CN201710013169A CN106532426B CN 106532426 B CN106532426 B CN 106532426B CN 201710013169 A CN201710013169 A CN 201710013169A CN 106532426 B CN106532426 B CN 106532426B
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01S—DEVICES USING THE PROCESS OF LIGHT AMPLIFICATION BY STIMULATED EMISSION OF RADIATION [LASER] TO AMPLIFY OR GENERATE LIGHT; DEVICES USING STIMULATED EMISSION OF ELECTROMAGNETIC RADIATION IN WAVE RANGES OTHER THAN OPTICAL
- H01S3/00—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range
- H01S3/30—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range using scattering effects, e.g. stimulated Brillouin or Raman effects
- H01S3/302—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range using scattering effects, e.g. stimulated Brillouin or Raman effects in an optical fibre
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Abstract
The present invention is suitable for optical image technology field, provides a kind of enhancement device of multi-photon imaging signal, including impulse generating unit, and the pumping pulse for generating preset repetition rate and pulsewidth is incident to orphan and generates unit；Orphan generates unit, for incident pumping pulse to be adjusted to the pumping pulse of preset polarization state and carries out palarization multiplexing processing, and generate soliton self-frequency sh phenomenon and obtain the high-energy orphan of two beam phase co-wavelengths and cross-polarization, two beam high-energy orphans are then incident to orphan's filter unit；Orphan's filter unit, for being filtered to two incident beam high-energy orphans, the orphan for obtaining preset wavelength is sent to laser scanning microscope system as excitation signal, so that the laser scanning microscope system carries out multi-photon imaging according to the excitation signal.The enhancement device provided through the invention can improve the repetition rate of orphan using polarization multiplexing to improve the signal level of multi-photon imaging signal.
Description
Technical field
The invention belongs to optical image technology field more particularly to a kind of enhancement devices of multi-photon imaging signal.
Background technique
Soliton self-frequency sh is the nonlinear optical effect based on stimulated Raman scattering in pulse, is that one kind can produce wavelength
The effective ways of tunable femtosecond pulse.There is many optical waveguide (mainly optical fiber) of anomalous dispersion may serve to generate orphan
Sub- self-frequency shift, such as standard single-mode fiber, refractive-index-guiding photonic crystal fiber, hollow core photonic bandgap fiber, large mode field optical fiber
With the photonic crystal stick of large mode field.It is based primarily upon standard single-mode fiber (such as the fused quartz light of optical material natural dispersion characteristic
Fine and fluoride fiber) it can produce and receive the orphan of burnt magnitude energy.Refractive-index-guiding photonic crystal fiber can be customized to appoint
The zero-dispersion wavelength of meaning, therefore the orphan generated can cover broader wave band, but orphan's energy is only capable of reaching Ya Najiao magnitude.
Hollow core photonic bandgap fiber can also be customized to arbitrary zero-dispersion wavelength, and orphan's energy of generation can be up to micro- burnt magnitude,
But it is significantly increased close to passband edge decaying, therefore orphan's wavelength tuning range only has tens nanometers.And large mode field optical fiber and
Photonic crystal stick has both the characteristics of broad band wavelength tuning range (several hundred nanometers) and high soliton pulse energy (receive more than 100 coke),
But the wavelength for being limited its orphan by the material dispersion of fused quartz can only be greater than 1.3 microns.
Multi-photon imaging needs the femto-second laser pulse of tunable wave length as excitation light source, and soliton self-frequency sh technology becomes
One of the ideal chose of multi-photon imaging.1700 nano waveband high energy can be obtained using the soliton self-frequency sh in photonic crystal stick
The orphan of amount, the three-photon micro-imaging technique inhibited using the soliton lamp -house as excitation light source and background, can be in work
Body mouse brain obtains maximum imaging depth, to clearly show subcortical structure.Before the poster presentation, to accomplish
The multi-photon imaging of this depth needs the technology using very invasive, for example removes the grey matter above tissue or past brain
It is inserted into the technology of endoscope.
The applicant has found that so far, the imaging depth of 1700 nano wavebands is (about in the practice of the invention
At 1.4 millimeters) mainly limited by the signal level generated in deep tissues, therefore how more effectively to enhance signal level
It is the key that improve imaging depth.From the angle of excitation light source, following two technology is mainly used at present: (1) using more
The photonic crystal stick of big core size carries out soliton self-frequency sh.Because orphan's energy is directly proportional with effective core area, fibre core ruler
Very little bigger, orphan's energy is higher.Two-photon and three-photon signal can be by formula Ss2∝E2F/ τ and S3∝E3f/τ2It obtains,
In, E represents pulse energy, and f represents pulse recurrence frequency, and τ represents pulsewidth.It can be seen that can be enhanced by core size
Signal.(2) dispersion compensation, that is, Pulse Width of Soliton on sample is compressed to minimum value.According to above-mentioned formula signal level
Also it can therefore improve.
Core size is improved to obtain bigger this technology of orphan's energy and eventually be damaged the limitation of threshold value.Peak value
Bulk damage effect caused by light intensity and the self-focusing effect generated more than fused quartz optical fiber self-focusing threshold limit value (about 4 megawatts)
It should be likely to that photonic crystal stick or optical fiber can be damaged.
Summary of the invention
Technical problem to be solved by the present invention lies in provide a kind of enhancement device of multi-photon imaging signal, it is desirable to provide
Improve the signal level of multi-photon imaging signal.
The invention is realized in this way a kind of enhancement device of multi-photon imaging signal, including impulse generating unit, orphan
Generate unit and orphan's filter unit:
The impulse generating unit, for generating the pumping pulse of preset repetition rate and pulsewidth, and by the pumping arteries and veins
It pours and is incident upon orphan's generation unit；
The orphan generates unit, after the incident pumping pulse is adjusted to the pumping pulse of preset polarization state
Carry out palarization multiplexing processing, and according to treated pumping pulse generate soliton self-frequency sh phenomenon obtain two beam phase co-wavelengths and
The high-energy orphan of cross-polarization, and high-energy orphan described in two beams is incident to orphan's filter unit；
Orphan's filter unit obtains preset wavelength for being filtered to two incident beam high-energy orphans
Orphan, and treated orphan as excitation signal is sent to laser scanning microscope system, so that the laser scanning is aobvious
Micro-system carries out multi-photon imaging according to the excitation signal.
Further, the impulse generating unit includes 1550 nano optical fibers lasers and the first silver mirror；
The 1550 nano optical fibers laser, for generating the pumping arteries and veins of 1 MHz repetition and 500 femtosecond pulsewidths
Punching, and the pumping pulse is incident to first silver mirror；
First silver mirror, which reflects incident pumping pulse, generates unit into the orphan.
Further, it includes pulse adjustment module and orphan's generation module that the orphan, which generates unit,；
The pulse adjusts module, for the incident pumping pulse to be adjusted to the pumping pulse of preset polarization state simultaneously
Palarization multiplexing processing is carried out, then pumping pulse is incident to orphan's generation module by treated；
Orphan's generation module, for treated according to incidence, pumping pulse generation soliton self-frequency sh is existing
As obtaining the high-energy orphan of two beam phase co-wavelengths and cross-polarization, and high-energy orphan described in two beams is incident to the orphan
Sub- filter unit.
Further, pulse adjustment module includes the first half-wave plate, the second silver mirror, third silver mirror, the 4th silver mirror, the
One polarizing beam splitter cube, the second polarizing beam splitter cube and the first lens；
First half-wave plate is used to adjust the polarization state of the incident pumping pulse, and by pumping pulse adjusted
It is incident to second silver mirror；
Second silver mirror is used to reflect incident polarization state pumping pulse adjusted into first polarization beam splitting
Cube；
Incident pumping pulse is divided into the first pumping subpulse and the second pumping by first polarizing beam splitter cube
Pulse, and the second pumping subpulse is incident to second polarizing beam splitter cube；
The first pumping subpulse is respectively after the third silver mirror and the 4th silver mirror carry out optical path adjustment, with described the
Two pump subpulse vertical incidence to second polarizing beam splitter cube；
Second polarizing beam splitter cube is used to carry out the first incident pumping subpulse and the second pumping subpulse
Beam is closed, generates the pumping pulse for the 1550 nanometers of cross-polarization collinearly transmitted, and 1550 collinearly transmitted nanometer are orthogonal
The pumping pulse of polarization is incident to first lens；
First lens are used for the pumping pulse to incident 1550 collinearly transmitted nanometer cross-polarization and carry out
It focuses, and the pumping pulse after focusing is incident to orphan's generation module.
Further, orphan's generation module is the non-polarization-maintaining photonic crystal stick that core diameter is 100 microns.
Further, it further includes that module occurs for optical path adjustment module and orphan that the orphan, which generates unit,；
The optical path adjusts module, for the incident pumping pulse to be adjusted to the pumping pulse of preset polarization state,
And polarization state pumping pulse adjusted is incident to the orphan, module occurs；
Module occurs for the orphan, for carrying out palarization multiplexing processing to incident polarization state pumping pulse adjusted,
Then according to treated, pumping pulse generates soliton self-frequency sh phenomenon, obtains the high energy of two beam phase co-wavelengths and cross-polarization
Orphan is measured, and high-energy orphan described in two beams is incident to orphan's filter unit.
Further, the optical path adjustment module includes the second half-wave plate, the 5th silver mirror and the second lens；
Second half-wave plate is used to adjust the polarization state of the pumping pulse by the impulse generating unit incidence, and
Pumping pulse adjusted is incident to the 5th silver mirror；
5th silver mirror is used to reflect incident polarization state pumping pulse adjusted into second lens；
Second lens are used to be focused incident pumping pulse, and the pumping pulse after focusing is incident to institute
It states orphan and module occurs.
Further, it is the polarization-maintaining large mode field optical fiber that core diameter is 40 microns that module, which occurs, for the orphan.
Further, orphan's filter unit includes the third lens and optical filter；
The third lens, for collimating two beam soliton pulses of incident diverging, so that the two beams orphan
It is incident to the optical filter in parallel；
The optical filter obtains the orphan of preset wavelength, and will filter for being filtered to the parallel orphan
The orphan obtained after wave processing is sent to laser scanning microscope system as excitation signal.
Further, the optical filter is long wave pass filter.
Compared with prior art, the present invention beneficial effect is: the embodiment of the present invention by generate preset repetition rate and
The pumping pulse of pulsewidth, and polarization state adjustment is carried out to the pumping pulse, polarization state pumping pulse adjusted will be carried out and carried out
Palarization multiplexing processing, and because soliton self-frequency sh phenomenon obtains two beam orphans of phase co-wavelength and cross-polarization, to two obtained
Beam orphan is filtered, and treated orphan as excitation signal is sent to laser scanning microscope system, sweeps laser
It retouches microscopic system and multi-photon imaging is carried out according to the excitation signal.The enhancement device provided through the embodiment of the present invention, can
Increase the repetition rate of orphan using polarization multiplexing to improve the signal level of multi-photon imaging signal.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of the enhancement device for multi-photon imaging signal that first embodiment of the invention provides；
Fig. 2 is a kind of detailed construction signal of the enhancement device for multi-photon imaging signal that second embodiment of the invention provides
Figure；
Horizontal state of polarization and polarization and vertical polarization are lonely in the non-polarization-maintaining photonic crystal stick that Fig. 3 provides for second embodiment of the invention
The measure spectrum and interference auto-correlation mark of son；
Fig. 4 is the three-photon fluorescent image in non-polarization-maintaining photonic crystal stick that second embodiment of the invention provides；
Fig. 5 is a kind of detailed construction signal of the enhancement device for multi-photon imaging signal that third embodiment of the invention provides
Figure；
Horizontal state of polarization and polarization and vertical polarization orphan in the polarization-maintaining large mode field optical fiber that Fig. 6 provides for third embodiment of the invention
Measure spectrum and interference auto-correlation mark；
Fig. 7 is the two-photon fluorescence imaging figure in polarization-maintaining large mode field optical fiber that third embodiment of the invention provides.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
Applicant of the present invention has found during the embodiment present invention, improves core size to obtain bigger orphan's energy
It measures this technology to be eventually damaged the limitation of threshold value, be limited once orphan's ceiling capacity is damaged threshold value, Pulse Width of Soliton
Also due to dispersion compensation and reached minimum value, it is desirable to enhance multi-photon imaging signal, unique controllable laser parameter is exactly to weigh
Complex frequency, because multi-photon imaging signal is proportional to repetition rate, the increase of repetition rate also can effectively improve mostly light
The signal level of subsignal is based on this, the present invention provides a kind of enhancement device of multi-photon imaging signal as shown in Figure 1,
Unit 2 and orphan's filter unit 3 are generated including impulse generating unit 1, orphan:
Impulse generating unit 1 enters for generating the pumping pulse of preset repetition rate and pulsewidth, and by the pumping pulse
It is incident upon orphan and generates unit 2；
Orphan generates unit 2, and the pumping pulse for the incident pumping pulse to be adjusted to preset polarization state is gone forward side by side
The processing of row palarization multiplexing, and soliton self-frequency sh phenomenon is generated according to treated pumping pulse and obtains two beam phase co-wavelengths and just
The high-energy orphan of polarization is handed over, and high-energy orphan described in two beams is incident to orphan's filter unit 3.
Orphan's filter unit 3 obtains the orphan of preset wavelength for being filtered to two incident beam high-energy orphans
Son, and treated orphan as excitation signal is sent to laser scanning microscope system, so that the scan laser microphotograph system
System carries out multi-photon imaging according to the excitation signal.
The enhancement device provided through the embodiment of the present invention, can using polarization multiplexing increase orphan's repetition rate from
And improve the signal level of multi-photon imaging signal.In the present embodiment, enhancement device utilizes polarization multiplexing, just two beams
The pulse of polarization is handed over spatially to be combined (but due to postponing so being separated on time), so relative to a branch of arteries and veins
Repetition rate is brought to become twice, and the two pulses of cross-polarization generate the orphan of two beam cross-polarizations because of soliton self-frequency sh
Son is spatially combined.
On the basis of first embodiment, the present invention provides second embodiment shown in Fig. 2, in which: impulse generating unit 1
Including 1550 nano optical fibers laser Fiber Laser and the first silver mirror M1；1550 nano optical fibers laser Fiber Laser,
The first silver mirror is incident to for generating the pumping pulse of 1 MHz repetition and 500 femtosecond pulsewidths, and by the pumping pulse
M1, the pumping pulse are 1550 nano wire polarized pump pulses；After first silver mirror M1 is reflected incident pumping pulse,
It is incident to orphan and generates unit 2.
It includes pulse adjustment module 21 and orphan's generation module 22 that orphan, which generates unit 2,；
Pulse adjusts module 21, and the pumping pulse for the incident pumping pulse to be adjusted to preset polarization state is gone forward side by side
The processing of row palarization multiplexing, then by treated, pumping pulse is incident to orphan's generation module 22；
Orphan's generation module 22, for treated according to incidence, pumping pulse generates soliton self-frequency sh phenomenon,
The high-energy orphan of two beam phase co-wavelengths and cross-polarization is obtained, and high-energy orphan described in two beams is incident to orphan's filtering
Unit 3.
It includes the first half-wave plate HWP1, the second silver mirror M2, third silver mirror M3, the 4th silver mirror M4, that pulse, which adjusts module 21,
One polarizing beam splitter cube PBS1, the second polarizing beam splitter cube PBS2 and the first lens L1；
First half-wave plate HWP1 is used to adjust the polarization state of the incident pumping pulse, and by pumping pulse adjusted
It is incident to the second silver mirror M2；Polarization state pumping pulse adjusted is incident to the first polarization beam splitting after the second silver mirror M2 reflection
Cube PBS1；Incident pumping pulse is divided into the first pumping subpulse and the second pumping by the first polarization beam splitting solid side PBS1
Subpulse, and the second pumping subpulse is incident to the second polarizing beam splitter cube PBS2；The first pumping subpulse difference
After third silver mirror M3 and the 4th silver mirror M4 carries out optical path adjustment, with the second pumping subpulse vertical incidence to the second polarization point
Beam cube PBS2；Second polarizing beam splitter cube PBS2 is used for the first incident pumping subpulse and the second pumping subpulse
It carries out conjunction beam, generates the pumping pulse for the 1550 nanometers of cross-polarization collinearly transmitted, and by 1550 collinearly transmitted nanometer
The pumping pulse of cross-polarization is incident to the first lens L1；First lens L1 is used to receive incident 1550 collinearly transmitted
The pumping pulse of rice cross-polarization is focused, and the pumping pulse after focusing is incident to orphan's generation module 22.
In a particular application, orphan's generation module 22 is the non-polarization-maintaining photonic crystal stick PC that core diameter is 100 microns
Rod, the non-polarization-maintaining photonic crystal stick PC rod, for treated according to incidence, soliton self-frequency sh occurs for pumping pulse
Phenomenon generates the high-energy orphan of two beam phase co-wavelengths and cross-polarization, and high-energy orphan described in two beams is incident to described
Orphan's filter unit 3.
Orphan's filter unit 3 includes the third lens L3 and optical filter LPF；The third lens L3, for by incident diverging
Two beam orphans collimate, so that the two beams orphan is incident to optical filter LPF in parallel；Optical filter LPF, for described parallel
Orphan be filtered, obtain the orphan of preset wavelength, and send out the orphan obtained after filtering processing as excitation signal
Give laser scanning microscope system LSM.Specifically, optical filter LPF is long wave pass filter.
It provides and a kind of is increased using polarization multiplexing in order to improve the signal level of multi-photon imaging, in the present embodiment
The enhancement device of soliton pulse repetition rate.Polarization multiplexing is usually to be used to generate double-colored soliton in polarization maintaining optical fibre,
Its physical principle is: the linear polarization pumping pulse light of input can be divided into the pumping pulse light of two beam cross-polarizations, and with difference
Speed respectively along optical fiber two main shafts propagate.This two beams pumping pulse light can all be generated due to soliton self-frequency sh phenomenon
Orphan.The polarization state of adjustment input line polarized pump pulsed light can be adjusted constantly between the wavelength of two beam cross-polarization orphans
Every.In the present embodiment, it is multiple that polarization is applied in the enhancement device of non-polarization-maintaining photonic crystal stick for including not polarization structure
With technology, phase co-wavelength, the high-energy orphan of cross-polarization can be equally produced.On this basis, the present embodiment will polarize
Multiplexing technology has been applied to three-photon and two photon imaging.By the experimental results showed that, excited most by polarization coupling light
Whole signal level is equal to the sum of the signal that two beam cross-polarization orphans excite respectively.It is therefore seen that in multi-photon imaging,
Polarization multiplexing can be improved signal level.
In the present embodiment, using 1550 nano optical fibers lasers of 1 MHz repetition and 500 femtosecond pulsewidths
(FLCPA-02CSZU, Calmar) is used as pump light source, generates the pumping arteries and veins of 1 MHz repetition and 500 femtosecond pulsewidths
Punching, the pumping pulse are 1550 nano wire polarized pump pulses.First half-wave plate HWP is for changing 1550 nano wire polarized pumps
The polarization state of Pu pulse, and the energy proportioning along two orthogonal polarization orientations is adjusted with this.Non- polarization-maintaining used by wherein
The core diameter of photonic crystal stick is 100 microns, and length is 44 centimetres (SC-1500/100-Si-ROD, NKT Photonics).
This non-polarization-maintaining photonic crystal stick is no polarization-maintaining structure, even if but can also be with by the polarization state of 1 meter of conveying length laser
It remains unchanged.Two polarizing beam splitter cubes are used to generate the 1550 nanometers of cross-polarization pumping pulses collinearly transmitted.This 1550
Nanometer cross-polarization pumping pulse is by generating soliton self-frequency sh in non-polarization-maintaining photonic crystal stick, using long wave pass filter
Orphan required for the present embodiment has been obtained later.The present embodiment additionally uses spectrometer (OSA203B) and takes for the present embodiment
The interference autocorrelation function analyzer built comes measure spectrum and pulsewidth.Finally the orphan after spectrometer and interference autocorrelation function analyzer sweeps in laser
It retouches and carries out multi-photon imaging in microscopic system (LSM).
In the present embodiment, polarization multiplexing is applied in non-polarization-maintaining photonic crystal stick first.Fig. 3 shows filter
The spectrum of horizontal state of polarization orphan ((a) in Fig. 3) and polarization and vertical polarization orphan ((b) in Fig. 3) and interference auto-correlation after wave
Mark.Under the conditions of maximum pump energy, two orphans are moved to 1620 nanometer wave strong points, and orphan's energy is respectively 73 to receive coke
Coke is received with 75.The interference auto-correlation mark of horizontal state of polarization and polarization and vertical polarization orphan that (c) and (d) in Fig. 3 is shown respectively, by
This can obtain pulsewidth.It is assumed that orphan is hyperbolic secant model, after deconvolution calculates, the pulsewidth measured is respectively 73
Femtosecond and 70 femtoseconds.
It is collinearly to propagate that one major advantage of polarization multiplexing, which is two orphans, this is for multi-photon imaging
With very big benefit, because the space coincidence of two beam exciting lights is most important to multi-photon imaging.In this embodiment, to quilt
The intravital mouse blood vessel of Texas-red dye marker carries out three-photon fluorescent imaging, and the letter of polarization multiplexing is proved with this
Number humidification.In order to quantify this signal enhancing, the average three-photon fluorescent signal that compared in same angiosomes is strong
Degree, as shown in the marked region of Fig. 4, coloration item shows signal level in Fig. 4, and scale is 50 microns.Horizontal state of polarization and perpendicular
Straight polarization state orphan is due to similar energy and pulsewidth, so the signal value generated is also approximately equal, respectively 28390 Hes
27970.When two orphans are incident on simultaneously carries out three-photon fluorescent excitation on sample, signal value 56980, substantially equal to two
The sum of signal value when a orphan excites respectively.Therefore, the polarization multiplexing in non-polarization-maintaining photonic crystal stick can be effectively
Improve multi-photon imaging signal.It is further to note that before non-polarization-maintaining photonic crystal stick, by two 1550 nanometers of polarizations
The transmitance of the polarisation multiplex system of beam-dividing cube and two silver mirrors composition is up to 94%.
In practical application, there is also polarization-maintaining photonic crystal sticks.Polarization multiplexing in this large mode field optical waveguide is more
It is easy to realize.In order to imitate this polarization-maintaining photonic crystal stick, the present invention also provides another implementations as shown in Figure 5
The specific structure of example, impulse generating unit 1 and orphan's filter unit 3 is identical as the second embodiment of above-mentioned offer, only in orphan
The specific structure that son generates unit 2 is different, in which:
It further includes that module 24 occurs for optical path adjustment module 23 and orphan that orphan, which generates unit 2,；
Optical path adjusts module 23, for the incident pumping pulse to be adjusted to the pumping pulse of preset polarization state, and
Polarization state pumping pulse adjusted is incident to orphan, module 24 occurs；
Module 24 occurs for orphan, for carrying out palarization multiplexing processing to incident polarization state pumping pulse adjusted, so
According to treated, pumping pulse generates soliton self-frequency sh phenomenon afterwards, obtains the high-energy of two beam phase co-wavelengths and cross-polarization
Orphan, and high-energy orphan described in two beams is incident to orphan's filter unit 3.
It includes the second half-wave plate HWP2, the 5th silver mirror M5 and the second lens L2 that optical path, which adjusts module 23,；Second half-wave plate
HWP2 is used to adjust the polarization state of the incident pumping pulse, and pumping pulse adjusted is incident to the 5th silver mirror M5；
Polarization state pumping pulse adjusted is incident to the second lens L2 after the 5th silver mirror M5 reflection；Second lens L2 is used for entering
The pumping pulse penetrated is focused, and the pumping pulse after focusing is incident to orphan, module 24 occurs.
It is polarization-maintaining large mode field optical fiber LMA Fiber that module 24, which occurs, for orphan；Polarization-maintaining large mode field optical fiber LMA Fiber, is used for
Palarization multiplexing processing is carried out to incident polarization state pumping pulse adjusted, then pumping pulse generates orphan according to treated
Sub- phenomenon of self-frequency shift, generates the high-energy orphan of two beam phase co-wavelengths and cross-polarization, and high-energy orphan described in two beams is entered
It is incident upon orphan's filter unit 3.
In Fig. 5, core diameter is used as 40 microns of polarization-maintaining large mode field optical fiber LMA Fiber.The present embodiment provides
Enhancement device in, it is only necessary to before polarization-maintaining large mode field optical fiber LMA Fiber plus a half-wave plate HWP2, it is generated just
Hand over polarization solitons that will propagate respectively along two main shafts of polarization-maintaining large mode field optical fiber LMA Fiber.With non-polarization-maintaining photonic crystal stick
Compare, generated by polarization-maintaining large mode field optical fiber, by filtered orphan can have more obvious modulated spectrum (in Fig. 6 (a) and
(b)), and interfere auto-correlation mark ((c) and (d) in Fig. 6).Two pumping pulses or orphan are in transmission process in the present embodiment
In have occurred Cross-phase Modulation, and this follows the situation in non-polarization-maintaining photonic crystal stick different, in non-polarization-maintaining photonic crystal stick
In because polarizing beam splitter cube introduce optical delay, two beam cross-polarization pumping pulses are kept completely separate in time.
Filtered horizontal polarization and the energy of vertical polarization solitons are respectively 17.1 to receive that burnt and 19.6 receive coke.It is calculated by deconvolution
Afterwards, the measurement pulsewidth of horizontal polarization orphan and vertical polarization solitons is respectively 71 femtoseconds and 74 femtoseconds.
Likewise, the present embodiment has used polarization multiplexing in polarization-maintaining large mode field optical fiber, carry out multi-photon imaging pair
Than proving effect of the technology to signal enhancing.The present embodiment compares the two-photon fluorescence signal of mouse tail tendon, and
Prove the technology to the applicability of different image modes with this.Horizontal polarization SOLITON EXCITATION light ((a) in Fig. 7) and vertical polarization
The signal value that SOLITON EXCITATION light ((b) in Fig. 7) generates is respectively 22340 and 27070, and in Fig. 7, coloration item shows signal water
Flat, scale is 50 microns.And exciting obtained signal value then simultaneously by two orphans enhances to 51600.This shows that polarization is multiple
The signal level of the multi-photon imaging signal using polarization-maintaining large mode field optical fiber equally can be enhanced with technology.
In order to improve the signal level of multi-photon imaging signal, the present invention provides above-described embodiment, wherein imaging signal
It is to be excited by the soliton generated in big mode field area optical waveguide by soliton self-frequency sh.With single polarization soliton
Excitation is compared, and polarization multiplexing is actually the repetition rate of orphan to be doubled, and then proportionally improve signal.It is this
Technology can be applied in polarization-maintaining photonic crystal in addition to that can apply in non-polarization-maintaining photonic crystal stick and polarization-maintaining large mode field optical fiber
In stick.The multi-photon imaging results of three-photon fluorescent imaging and two-photon fluorescence imaging both modes all with the phase of the present embodiment
Meet, i.e. palarization multiplexing signal level that multi-photon imaging signal can be improved.It, can in applying non-polarization-maintaining photonic crystal stick
To increase more polarizing beam splitter cubes pair, the repetition rate of orphan proportional will be improved.For example, increasing a pair of of polarization point
Beam cube, repetition rate will add additional twice, and multi-photon imaging signal also increases twice, and total transmitance also can be compared with
Height, because each pair of polarizing beam splitter cube merely adds 6% loss on power.In addition, from non-polarization-maintaining photonic crystal stick
The orphan of output be it is conllinear, convenient for subsequent multi-photon be imaged.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (6)
1. a kind of enhancement device of multi-photon imaging signal, which is characterized in that including impulse generating unit, orphan generate unit and
Orphan's filter unit:
The impulse generating unit enters for generating the pumping pulse of preset repetition rate and pulsewidth, and by the pumping pulse
It is incident upon the orphan and generates unit；
The orphan generates unit, for carrying out after the incident pumping pulse is adjusted to the pumping pulse of preset polarization state
Palarization multiplexing processing, and soliton self-frequency sh phenomenon is generated according to treated pumping pulse and obtains two beam phase co-wavelengths and orthogonal
The high-energy orphan of polarization, and high-energy orphan described in two beams is incident to orphan's filter unit；
Orphan's filter unit obtains the orphan of preset wavelength for being filtered to two incident beam high-energy orphans
Son, and treated orphan as excitation signal is sent to laser scanning microscope system, so that the scan laser microphotograph system
System carries out multi-photon imaging according to the excitation signal；
It includes pulse adjustment module and orphan's generation module that the orphan, which generates unit,；
The pulse adjusts module, for the incident pumping pulse to be adjusted to pumping pulse and the progress of preset polarization state
Palarization multiplexing processing, then by treated, pumping pulse is incident to orphan's generation module；
Orphan's generation module, for treated according to incidence, pumping pulse generates soliton self-frequency sh phenomenon, obtains
To two beam phase co-wavelengths and the high-energy orphan of cross-polarization, and high-energy orphan described in two beams is incident to the orphan and is filtered
Wave unit；
Orphan's generation module is non-polarization-maintaining photonic crystal stick.
2. enhancement device as described in claim 1, which is characterized in that the impulse generating unit swashs including 1550 nano optical fibers
Light device and the first silver mirror；
The 1550 nano optical fibers laser, for generating the pumping pulse of 1 MHz repetition and 500 femtosecond pulsewidths, and
The pumping pulse is incident to first silver mirror；
First silver mirror, which reflects incident pumping pulse, generates unit into the orphan.
3. enhancement device as described in claim 1, which is characterized in that pulse adjustment module includes the first half-wave plate, the
Two silver mirrors, third silver mirror, the 4th silver mirror, the first polarizing beam splitter cube, the second polarizing beam splitter cube and the first lens；
First half-wave plate is used to adjust the polarization state of the incident pumping pulse, and pumping pulse adjusted is incident
To second silver mirror；
Second silver mirror is used to reflect incident polarization state pumping pulse adjusted into first polarization beam splitting cube
Body；
Incident pumping pulse is divided into the first pumping subpulse and the second pumping subpulse by first polarizing beam splitter cube,
And the second pumping subpulse is incident to second polarizing beam splitter cube；
The first pumping subpulse after the third silver mirror and the 4th silver mirror carry out optical path adjustment, pumps respectively with described second
Pu subpulse vertical incidence is to second polarizing beam splitter cube；
Second polarizing beam splitter cube is used to the first incident pumping subpulse and the second pumping subpulse carrying out conjunction beam,
Generate the pumping pulse of 1550 nanometers of cross-polarization collinearly transmitted, and by 1550 collinearly transmitted nanometer cross-polarization
Pumping pulse is incident to first lens；
First lens are used to be focused the pumping pulse of incident 1550 collinearly transmitted nanometer cross-polarization,
And the pumping pulse after focusing is incident to orphan's generation module.
4. enhancement device as claimed in claim 3, which is characterized in that orphan's generation module is that core diameter is 100 micro-
Rice, the non-polarization-maintaining photonic crystal stick that length is 44 centimetres, the polarization state of laser maintains not in the non-polarization-maintaining photonic crystal stick
Become.
5. enhancement device as described in claim 1, which is characterized in that orphan's filter unit includes the third lens and optical filtering
Piece；
The third lens, for collimating two beam soliton pulses of incident diverging, so that the two beams orphan is parallel
It is incident to the optical filter；
The optical filter obtains the orphan of preset wavelength, and will be at filtering for being filtered to the parallel orphan
The orphan obtained after reason is sent to laser scanning microscope system as excitation signal.
6. enhancement device as claimed in claim 5, which is characterized in that the optical filter is long wave pass filter.
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| CN107144451B (en) * | 2017-05-10 | 2020-02-11 | 深圳大学 | Heavy water sealing method and method for detecting multi-photon signal intensity in multi-photon imaging |
| CN108469412A (en) * | 2018-03-26 | 2018-08-31 | 深圳大学 | The self-reference measuring device of axial chromatic aberration in a kind of multiphoton microscope |
| CN109638616B (en) * | 2018-12-11 | 2019-10-29 | 杭州奕力科技有限公司 | Ultrashort pulse energy stability measuring device based on fibre-optical dispersion glistening light of waves orphan |
| CN109814282B (en) * | 2019-03-26 | 2022-05-13 | 深圳大学 | Rod-shaped photonic crystal fiber-based soliton synthesis method and device |
| CN110221445A (en) * | 2019-06-25 | 2019-09-10 | 深圳大学 | Circular polarization orphan generation device and multi-photon micro imaging system |
| CN113916855A (en) * | 2021-09-29 | 2022-01-11 | 深圳大学 | Microscopic imaging device |
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