WO2020147604A1 - Procédé de conservation de type d'étanchéisation et de congélation à ultra basse température - Google Patents

Procédé de conservation de type d'étanchéisation et de congélation à ultra basse température Download PDF

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Publication number
WO2020147604A1
WO2020147604A1 PCT/CN2020/070482 CN2020070482W WO2020147604A1 WO 2020147604 A1 WO2020147604 A1 WO 2020147604A1 CN 2020070482 W CN2020070482 W CN 2020070482W WO 2020147604 A1 WO2020147604 A1 WO 2020147604A1
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WO
WIPO (PCT)
Prior art keywords
container
sterile
ultra
opening
low temperature
Prior art date
Application number
PCT/CN2020/070482
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English (en)
Chinese (zh)
Inventor
彭秋平
薛松果
王荣祥
彭文林
胥尧
Original Assignee
上海廪典实业有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 上海廪典实业有限公司 filed Critical 上海廪典实业有限公司
Publication of WO2020147604A1 publication Critical patent/WO2020147604A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen

Definitions

  • cold sources such as commercially available liquid nitrogen may be contaminated by pathogenic microorganisms such as bacteria, viruses, and fungi during production, transportation, and long-term storage in liquid nitrogen tanks and other storage tanks.
  • Pathogenic microorganisms can tolerate low temperatures and have the risk of contamination and cross-infection after resuscitation, so embryos stored in open vitrification have potential risks of contamination.
  • closed pull straws CPS, closed pulled straws
  • straw-in-straw straw-in-straw
  • Rapid-i closed vitrification methods are used in assisted reproduction clinics , But the survival rate is poor and it is not widely used.
  • the aseptic container is subjected to aseptic treatment before use, for example, treatment with high temperature, ultraviolet light and/or ethylene oxide.
  • the material of the aseptic container includes but is not limited to metal, plastic, foam, glass, etc.
  • the present invention also provides an embodiment in which the sterile liquid air prepared by the method of the present invention is used as a cold source to achieve long-term aseptic preservation of embryos, including the following steps: an outer tube is used as a sterile container, wherein the outer tube may be made of metal, Such as medical stainless steel, a precision screw is set at the opening, and the freezing rod loaded with embryos is put into the outer tube to complete vitrification and then tightened with the corresponding precision nut to achieve a spiral seal (both internal or external rotation), and transfer to Long-term storage in conventional liquid nitrogen tanks can realize safe, sterile, and long-term storage of biological samples without cross contamination.
  • the outer sleeve may also be made of plastic material.
  • the liquid nitrogen in the liquid nitrogen tank is not clean (commercial liquid nitrogen contains bacteria, viruses, fungi and other pathogenic microorganisms), the embryos are vitrified and frozen in sterile liquid air and stored in the liquid nitrogen tank. Therefore, the external non-clean liquid nitrogen will not affect the sterile state of the frozen embryos.
  • Step 3 A step of removing moisture or mist at the opening 10 of the sterile container. Specifically, during the process of preparing a sterile cold source, water vapor may be deposited at the opening 10. Before the opening 10 is sealed, a heat source should be used close to the opening 10 or a sterile gauze should be used to wipe the first opening 10, etc. The water vapor is removed so as to seal the first opening 10 easily.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Hematology (AREA)
  • Mechanical Engineering (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un procédé de conservation de type d'étanchéisation et de congélation à ultra basse température, comprenant les étapes suivantes consistant : 1) à fournir un récipient aseptique avec une ouverture ; 2) à placer le récipient aseptique dans une source froide initiale, la hauteur de l'ouverture du récipient aseptique étant plus haute qu'une couche de niveau de liquide de la source froide initiale ; 3) à former un gaz liquéfié sous la forme d'un fluide frigorigène aseptique dans le récipient aseptique ; 4) à placer un échantillon à congeler, supporté sur un support, dans le fluide frigorigène aseptique pour la congélation par vitrification ; et 5) à sceller le récipient aseptique et à effectuer une conservation à ultra basse température du récipient aseptique scellé, conjointement avec l'échantillon congelé par vitrification scellé à l'intérieur de celui-ci.
PCT/CN2020/070482 2019-01-18 2020-01-06 Procédé de conservation de type d'étanchéisation et de congélation à ultra basse température WO2020147604A1 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
CN201920090123.7 2019-01-18
CN201920090123 2019-01-18
CN201910074096.9 2019-01-25
CN201910074096 2019-01-25
CN201910709712.3A CN110521720B (zh) 2019-01-18 2019-08-02 一种超低温冷冻及封闭式保存方法
CN201910709712.3 2019-08-02

Publications (1)

Publication Number Publication Date
WO2020147604A1 true WO2020147604A1 (fr) 2020-07-23

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2020/070482 WO2020147604A1 (fr) 2019-01-18 2020-01-06 Procédé de conservation de type d'étanchéisation et de congélation à ultra basse température

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Country Link
CN (1) CN110521720B (fr)
WO (1) WO2020147604A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110521720B (zh) * 2019-01-18 2020-06-16 上海廪典实业有限公司 一种超低温冷冻及封闭式保存方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104719283A (zh) * 2015-03-24 2015-06-24 阳艳群 一种封闭式冷冻载体及其使用方法
CN205695269U (zh) * 2016-04-26 2016-11-23 同济大学苏州研究院 一种双封闭式卵子或胚胎玻璃化冷冻保存装置
CN107372470A (zh) * 2017-09-11 2017-11-24 马博 一种卵子胚胎玻璃化冷冻保存装置
CN208159973U (zh) * 2018-03-05 2018-11-30 河北医科大学第二医院 一种新型封闭式玻璃化冷冻载杆
CN110521720A (zh) * 2019-01-18 2019-12-03 上海廪典实业有限公司 一种超低温冷冻及封闭式保存方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103190393B (zh) * 2013-04-09 2015-05-13 上海安久生物科技有限公司 一种生物样本玻璃化冷冻载体及其用途
CN205143336U (zh) * 2015-05-19 2016-04-13 上海交通大学医学院附属第九人民医院 一种微量***冷冻保存载体

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104719283A (zh) * 2015-03-24 2015-06-24 阳艳群 一种封闭式冷冻载体及其使用方法
CN205695269U (zh) * 2016-04-26 2016-11-23 同济大学苏州研究院 一种双封闭式卵子或胚胎玻璃化冷冻保存装置
CN107372470A (zh) * 2017-09-11 2017-11-24 马博 一种卵子胚胎玻璃化冷冻保存装置
CN208159973U (zh) * 2018-03-05 2018-11-30 河北医科大学第二医院 一种新型封闭式玻璃化冷冻载杆
CN110521720A (zh) * 2019-01-18 2019-12-03 上海廪典实业有限公司 一种超低温冷冻及封闭式保存方法

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Publication number Publication date
CN110521720A (zh) 2019-12-03
CN110521720B (zh) 2020-06-16

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