CN110521720B - 一种超低温冷冻及封闭式保存方法 - Google Patents
一种超低温冷冻及封闭式保存方法 Download PDFInfo
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- CN110521720B CN110521720B CN201910709712.3A CN201910709712A CN110521720B CN 110521720 B CN110521720 B CN 110521720B CN 201910709712 A CN201910709712 A CN 201910709712A CN 110521720 B CN110521720 B CN 110521720B
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- container
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- freezing
- low temperature
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- 238000007710 freezing Methods 0.000 title claims abstract description 65
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- 239000007788 liquid Substances 0.000 claims abstract description 100
- 239000003507 refrigerant Substances 0.000 claims abstract description 34
- 238000007789 sealing Methods 0.000 claims abstract description 31
- 238000004017 vitrification Methods 0.000 claims abstract description 25
- 239000000523 sample Substances 0.000 claims abstract description 20
- 239000012520 frozen sample Substances 0.000 claims abstract description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 118
- 229910052757 nitrogen Inorganic materials 0.000 claims description 58
- 238000003860 storage Methods 0.000 claims description 14
- 210000004681 ovum Anatomy 0.000 claims description 5
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- 239000000463 material Substances 0.000 claims description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 claims description 2
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- 210000002257 embryonic structure Anatomy 0.000 description 24
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- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Hematology (AREA)
- Mechanical Engineering (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2020/070482 WO2020147604A1 (zh) | 2019-01-18 | 2020-01-06 | 一种超低温冷冻及封闭式保存方法 |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201920090123 | 2019-01-18 | ||
CN2019200901237 | 2019-01-18 | ||
CN201910074096 | 2019-01-25 | ||
CN2019100740969 | 2019-01-25 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110521720A CN110521720A (zh) | 2019-12-03 |
CN110521720B true CN110521720B (zh) | 2020-06-16 |
Family
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CN201910709712.3A Active CN110521720B (zh) | 2019-01-18 | 2019-08-02 | 一种超低温冷冻及封闭式保存方法 |
Country Status (2)
Country | Link |
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CN (1) | CN110521720B (zh) |
WO (1) | WO2020147604A1 (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110521720B (zh) * | 2019-01-18 | 2020-06-16 | 上海廪典实业有限公司 | 一种超低温冷冻及封闭式保存方法 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103190393B (zh) * | 2013-04-09 | 2015-05-13 | 上海安久生物科技有限公司 | 一种生物样本玻璃化冷冻载体及其用途 |
CN104719283A (zh) * | 2015-03-24 | 2015-06-24 | 阳艳群 | 一种封闭式冷冻载体及其使用方法 |
CN205143336U (zh) * | 2015-05-19 | 2016-04-13 | 上海交通大学医学院附属第九人民医院 | 一种微量***冷冻保存载体 |
CN205695269U (zh) * | 2016-04-26 | 2016-11-23 | 同济大学苏州研究院 | 一种双封闭式卵子或胚胎玻璃化冷冻保存装置 |
CN107372470B (zh) * | 2017-09-11 | 2021-05-28 | 马博 | 一种卵子胚胎玻璃化冷冻保存装置 |
CN208159973U (zh) * | 2018-03-05 | 2018-11-30 | 河北医科大学第二医院 | 一种新型封闭式玻璃化冷冻载杆 |
CN110521720B (zh) * | 2019-01-18 | 2020-06-16 | 上海廪典实业有限公司 | 一种超低温冷冻及封闭式保存方法 |
-
2019
- 2019-08-02 CN CN201910709712.3A patent/CN110521720B/zh active Active
-
2020
- 2020-01-06 WO PCT/CN2020/070482 patent/WO2020147604A1/zh active Application Filing
Also Published As
Publication number | Publication date |
---|---|
CN110521720A (zh) | 2019-12-03 |
WO2020147604A1 (zh) | 2020-07-23 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20230222 Address after: 222199 No. 18, Jinqiao Road, Dapu Industrial Zone, Lianyungang Economic and Technological Development Zone, Lianyungang City, Jiangsu Province Patentee after: Jiangsu RUIFUDA Medical Instrument Co.,Ltd. Address before: 301 Qianxin Road, Jinshanwei Town, Jinshan District, Shanghai, 201515 Patentee before: Shanghai Lindian Industrial Co.,Ltd. |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A method of ultra-low temperature freezing and closed storage Granted publication date: 20200616 Pledgee: Lianyungang branch of Bank of Jiangsu Co.,Ltd. Pledgor: Jiangsu RUIFUDA Medical Instrument Co.,Ltd. Registration number: Y2024980003340 |