WO2019157850A1 - 一种培养皿移液装置 - Google Patents

一种培养皿移液装置 Download PDF

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Publication number
WO2019157850A1
WO2019157850A1 PCT/CN2018/118489 CN2018118489W WO2019157850A1 WO 2019157850 A1 WO2019157850 A1 WO 2019157850A1 CN 2018118489 W CN2018118489 W CN 2018118489W WO 2019157850 A1 WO2019157850 A1 WO 2019157850A1
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WO
WIPO (PCT)
Prior art keywords
pipette
petri dish
pipetting device
strut
disposed
Prior art date
Application number
PCT/CN2018/118489
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English (en)
French (fr)
Inventor
程晓东
Original Assignee
程晓东
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN201820257238.6U external-priority patent/CN208104385U/zh
Priority claimed from CN201810274228.8A external-priority patent/CN108251289B/zh
Application filed by 程晓东 filed Critical 程晓东
Publication of WO2019157850A1 publication Critical patent/WO2019157850A1/zh

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology

Definitions

  • the present disclosure relates to the technical field of experimental equipment, and in particular to a culture dish pipetting device.
  • the existing cell culture liquid exchange operation mainly involves manual liquid exchange and workstation liquid exchange.
  • the manual fluid change is usually carried out by hand-pulling the culture dish with a row of guns equipped with a row of pipettes, and the station changeover can be carried out in batches for rapid pipetting.
  • the device provided by the present disclosure can also achieve a fast and accurate pipetting effect by being used in conjunction with a pump.
  • the petri dish pipetting device comprises a plurality of pipettes arranged in a vertical direction and arranged in a straight line in a lateral direction, the bottom end of the pipette is set as a suction nozzle, and the end away from the nozzle is an interface.
  • the utility model further comprises transversely fixing the lateral connection of the pipette, and the side wall of any one side of the transverse connection is fixedly connected with the central side wall of each liquid pipe. Both ends of the transverse connection are connected with struts extending perpendicularly to the suction nozzle of the pipette, the length of the struts is uniform, and is longer than the pipette.
  • the transverse tube comprises a horizontal tube that laterally communicates with the interface of the pipette, and the horizontal tube is a hollow structure that is closed at both ends.
  • the horizontal pipe is disposed at one end of each of the liquid suction pipes near the interface, and a convex mouth communicating with the inside of the horizontal pipe is disposed on the side of the interface corresponding to the liquid suction pipe, and each of the convex nozzles is coupled with the corresponding interface.
  • the middle portion of the horizontal pipe away from the pipette is provided with a vacuum pump port communicating with the inside of the horizontal pipe.
  • the struts are connected to the transverse joint as a unitary structure.
  • the transverse and transverse tubes or one of them is integral with the pipette.
  • the strut and the cross tube are separated structures, and the strut is connected to both ends of the cross tube through a connector, and the connector comprises a socket disposed at two ends of the cross tube, and a plug disposed at one end of the strut corresponding to the socket.
  • the socket of the connector is a cylindrical groove disposed at both ends of the horizontal tube and concave along the axial direction of the horizontal tube, and the inner wall of the cylindrical groove is provided with a positioning groove disposed along the circumferential direction of the cylindrical groove;
  • a cylindrical projection corresponding to the cylindrical recess At the top end of the strut, a cylindrical projection corresponding to the cylindrical recess, the four walls of the cylindrical projection are circumferentially provided with positioning ribs corresponding to the positioning grooves.
  • the outer edge of the cylindrical groove at both ends of the horizontal pipe is provided with a notch
  • the cylindrical protruding head is provided with a corresponding notch protrusion near one end of the strut, and the protrusion and the notch are nested when the strut is set in parallel with the pipette Cooperate.
  • the cylindrical recess of the connector is disposed in a vertical direction, and the slot is perpendicular to the direction of the nozzle of the pipette; the cylindrical boss is disposed at the top end of the strut in the direction of the strut axis, and is cylindrically concave Corresponding fit of the slot.
  • the struts at both ends of the transverse connection are provided with rupture grooves at the same height.
  • the rupture groove is provided in plurality, and the plurality of rupture grooves are sequentially disposed along the vertical direction of the struts.
  • the plurality of rupture grooves are disposed at equal intervals along the vertical direction of the struts.
  • the rupture groove is disposed obliquely, and the open end of the rupture groove is disposed lower than the break point of the rupture groove, and is configured such that the open end of the rupture groove is a contact portion of the struts.
  • the strut comprises a plurality of splicing strut segments, and the plurality of splicing strut segments are sequentially connected in the first position.
  • the contour adjustment mechanism comprises a height adjustment mechanism comprising a foot pad and a square tube vertically disposed above the rubber pad; the opposite side walls of the square tube are respectively provided with a sliding plate axially sliding along the square tube, and the sliding plate is opposite One side is respectively provided with teeth; two slide plates extend outward through the bottom end of the square tube, and are respectively hinged to the two sides of the rubber pad through the hinge shaft; the driving gear is arranged between the sliding plates, and the teeth on both sides of the driving gear are driven The teeth are respectively meshed with the teeth of the sliding plate, and the driving gear is connected to the inner arm of the square tube through the shaft; one end of the driving gear is coaxially connected with the first bevel gear, and the end of the square tube at the driving gear away from the foot pad is coaxially provided with a reciprocating screw.
  • the end of the reciprocating screw near the driving gear meshes with the first bevel gear through the second bevel gear;
  • the reciprocating screw sidewall surface is provided with a reciprocating groove;
  • the square tube is further provided with a telescopically arranged telescopically disposed between the square pipe and the reciprocating screw
  • the inner wall of the cylinder, the telescopic cylinder and the reciprocating screw is provided with a reciprocating slider which cooperates with the bottom end of the reciprocating groove; the top of the telescopic cylinder is connected with the bottom of the strut.
  • the second bevel gear is coaxially connected to the reciprocating screw through the journal; the inner side wall of the square tube that does not overlap with the sliding plate is provided with a baffle that cooperates with the journal shaft hole, and the thickness of the baffle is consistent with the length of the journal.
  • the method further comprises a contour adjustment mechanism
  • the contour adjustment mechanism comprises a foot pad, a square tube vertically disposed above the rubber pad; the opposite side walls of the square tube are respectively provided with a sliding plate sliding along the axial direction of the square tube, the sliding plate The opposite side is provided with inclined teeth with the same inclination and modulus; the two slides extend outward through the bottom end of the square tube, and are respectively hinged to the two sides of the rubber pad through the hinge shaft; the bottom of the reciprocating screw is coaxial
  • the connecting gear is provided with a helical gear, the helical gear meshes with the helical tooth of the sliding plate; the one end of the helical gear away from the reciprocating screw is coaxially connected with a shaft head, the side wall of the shaft head is provided with a circumferential slot; the inner side of the square tube does not overlap with the inner side of the sliding plate
  • the wall is provided with a positioning block that cooperates with the slot of the shaft head.
  • the present disclosure provides a petri dish pipetting device comprising a plurality of pipettes arranged in a vertical direction and arranged in a straight line in a lateral direction, the bottom end of the pipette is configured as a suction nozzle, and an end away from the nozzle is an interface;
  • the utility model further comprises transversely fixing the horizontal connection of the liquid suction pipe, and the side wall of any one side of the transverse connection is fixedly connected with the central side wall of each liquid suction pipe; the two ends of the transverse connection are connected to be perpendicular to the suction nozzle of the liquid suction pipe.
  • the extended struts have the same length and are shorter than the pipette.
  • the struts are shorter than the length of the pipette in the range of 0-50 mm.
  • the present disclosure provides a petri dish pipetting device comprising a plurality of pipettes arranged in a vertical direction and arranged in a straight line in a lateral direction, the bottom end of the pipette is configured as a suction nozzle, and an end away from the nozzle is an interface;
  • the utility model further comprises a transversely fixed suction pipe cross-section, the side wall of any one of the transverse joints is fixedly connected with the central side wall of each of the liquid suction pipes, and the bottom surface of the transverse connection can be in contact with the upper surface or the edge of the culture dish .
  • the present disclosure maintains the left and right vertical angles of the liquid absorbing device by the support frames disposed on both sides of the pipette, so that it is easy to grasp the balance during use, so that each nozzle is at the same level. Height, so in the process of pipetting, the remaining liquid level can be made uniform, which ensures the consistency of the experiment.
  • the support frame can be integrally arranged with the liquid suction pipe, and can also be detachable and replaceable by the connection mechanism, and can support the support frame of different size heights, thereby achieving the purpose of reducing cost and flexible application.
  • the present disclosure also compensates for the vertical distance change between the suction nozzle and the liquid surface caused by the tilting of the pipette by the tilt compensation mechanism, thereby ensuring that the remaining amount of pipetting is the same as the planned remaining, and rejecting the movement caused by the inclination of the pipette
  • the liquid volume change problem ensures the accuracy of pipetting.
  • the present disclosure also proposes a structure in which the reciprocating screw is directly driven by the helical gear, and for this, the manufacturing cost of the tilt compensation mechanism is reduced.
  • Figure 1 is a schematic view of a pipette device for a culture tray of a joint struts
  • FIG. 2 is a schematic view of a petri dish pipetting device of a split struts
  • Figure 3 is a schematic view of a strut connector
  • Figure 4 is a schematic view of the tilt compensation mechanism
  • Figure 5 is an internal cross-sectional view of the tilt compensation mechanism
  • FIG. 6 is a schematic structural view of an improvement scheme of a tilt compensation mechanism
  • Figure 7 is a schematic view of a transverse connection structure
  • Figure 8 is a schematic view of the positioning mechanism of the strut connector
  • Figure 9 is a schematic view showing the structure of the strut shorter than the pipette
  • Figure 10 is a schematic view showing the structure of a horizontally connected fixed pipette.
  • Icons 1-pipette; 2-nozzle; 3-port; 32-cross tube; 4-crosslink; 41-check valve; 5-spigot; 6-strut; 7-vacuum pump port; Fracture groove; 11-cylindrical groove; 12-cylindrical nose; 13-positioning groove; 14-positioning rib; 15-notch; 16-bump; 17-foot pad; 18-square tube; 20-spur gear; 21-first bevel gear; 22-second bevel gear; 23-reciprocating screw; 24-reciprocating groove; 25-reciprocating slider; 26-baffle; 27- journal; 28- helical gear ; 29-axis head; 30-positioning block; 31- telescopic cylinder.
  • the terms “installation”, “connected”, and “connected” should be understood broadly, for example, may be a fixed connection, or may be Removable connection, or integral connection; may be mechanical connection or electrical connection; may be directly connected, or may be indirectly connected through an intermediate medium, and may be internal communication between the two elements.
  • installation should be understood broadly, for example, may be a fixed connection, or may be Removable connection, or integral connection; may be mechanical connection or electrical connection; may be directly connected, or may be indirectly connected through an intermediate medium, and may be internal communication between the two elements.
  • the manual replacement of the prior art has the following disadvantages: in the process of changing the hand-held gun, it is necessary to hold the gun smoothly and keep the suction port of the gun at a fixed height, but due to the shaking of the hand and the lack of support points, etc. The reason is that in the process of holding the gun, it is difficult to keep the nozzle washing liquid at a fixed height, so that the remaining liquid amount does not reach the target requirement, and it takes time and effort, and it is easy to introduce pollution to influence the experimental result;
  • the workstation liquid exchange can overcome the lack of precision of manual liquid exchange, its equipment expenditure is huge, the instrument investment is huge, and it is not suitable for large-area promotion and application. Usually, the cost of consumables is much higher than the cost of manual replacement.
  • FIG. 1 , FIG. 2 , FIG. 3 , FIG. 4 , FIG. 5 , FIG. 6 , FIG. 7 and FIG. 8 including a plurality of pipettes 1 arranged in a vertical direction and arranged in a straight line in the transverse direction, and the pipette 1
  • the bottom end is set as the suction nozzle 2, and the end away from the suction nozzle 2 is the interface 3.
  • It also includes a transverse joint 4 for laterally fixing the pipette 1, and the side walls on either side of the transverse joint 4 are fixedly connected to the central side wall of each of the pipettes 1. Both ends of the transverse joint 4 are connected with struts 6 extending vertically in the direction of the suction nozzle 2 of the pipette 1, and the struts 6 have the same length and longer than the pipette 1.
  • the length of the strut 6 is higher than the length of the pipette 1 is 0-50 mm (excluding the case where the strut and the pipette are equal).
  • the transverse joint 4 includes a transverse tube 32 that laterally communicates with the interface 3 of the pipette 1, and the horizontal tube 32 is a hollow structure that is closed at both ends.
  • the horizontal pipe 32 is disposed at one end of each of the liquid suction pipes 1 near the interface, and a convex nozzle 5 communicating with the inside of the horizontal pipe 32 is disposed on the side of the interface 3 corresponding to the liquid suction pipe 1, and each of the convex nozzles 5 is connected with the corresponding interface 3.
  • a vacuum pump port 7 communicating with the inside of the horizontal pipe 32 is provided at a central portion of the horizontal pipe 32 away from the pipette 1.
  • the transverse joint 4 and the pipette 1 are of unitary construction.
  • the strut 6 and the cross tube 32 and the transverse joint 4 may be of a unitary structure; alternatively, the strut 6 and the cross tube 32 may also have a split structure.
  • the operator connects the petri dish pipetting device to the vacuum pump through the vacuum pump port 7, and contacts the petri dish base or the test bench table through the strut 6, so that the left and right sides of the pipette 1 are equal.
  • the nozzles are of the same height.
  • the pipette 1 can draw excess liquid from the dish and retain as much of the culture.
  • the struts 6 and the transverse joints 4 can be an integral structure.
  • the liquid changing device is simple and reliable to use, and the struts 6 at both ends are highly uniform and highly accurate in the manufacturing process, and the manual adjustment step is omitted.
  • the strut 6 and the cross-connect 4 may also be a detachable split structure.
  • the strut 6 is connected to the two ends of the cross-connect 4 through a connector, and the connector is disposed in the cross-connect 4
  • the sockets at both ends further include a plug disposed at one end of the strut 6 corresponding to the socket.
  • the socket of the connector is a cylindrical groove 11 which is disposed at both ends of the transverse connection 4 and is concave along the transverse direction 4, and the inner wall of the groove is provided with a positioning groove 13 circumferentially disposed along the cylindrical groove 11.
  • the struts 6 include a cylindrical boss 12 disposed at the top end of the struts 6 corresponding to the cylindrical recess 11.
  • the four ribs of the cylindrical boss 12 are circumferentially provided with positioning ribs 14 corresponding to the positioning grooves 13, and different heights can be used during use.
  • the struts 6 are connected at both ends of the transverse joint 4 and are firmly fixed, so that not only the requirements for adapting the height of the culture dish and different liquid levels can be achieved, but the split struts 6 can be repeatedly used to save materials.
  • the connector in order to prevent the rotation of the strut 6 on the transverse joint 4 from affecting the working precision during installation and use, the connector includes a notch 15 provided at the outer edge of the cylindrical recess 11 at both ends of the transverse joint 4, Also included is a projection 16 disposed on the cylindrical boss 12 adjacent to the end of the struts 6 corresponding to the notch 15, and only when the struts 6 are mounted in parallel with the pipette 1, the projections 16 are nested with the notches 15 and the cylindrical projections 12 will be smoothly inserted into the cylindrical recess 11 to achieve the purpose of angle constraint.
  • the connector can be set in the axial direction of the strut 6, at which time the notch of the cylindrical recess 11 is directed perpendicular to the suction nozzle 2 of the pipette 1.
  • the cylindrical boss 12 is disposed at the top end of the stay 6 in the axial direction of the stay 6 and correspondingly cooperates with the cylindrical recess 11.
  • the strut 6 can also be a multi-segment splicing structure, so the struts 6 are arranged in pairs of different groups according to different lengths, and the tops of each pair of struts 6 are coaxial.
  • a cylindrical boss 12 corresponding to the cylindrical recess 11 is provided, and the bottom of each pair of struts 6 is also provided with a corresponding cylindrical recess 11 so that the plurality of struts 6 can be connected end to end for length adjustment In order to achieve the effect of flexible use in the experimental requirements.
  • the struts 6 at both ends of the transverse joint 4 are provided with rupture grooves 8 at the same height.
  • a plurality of rupture grooves 8 are provided, and a plurality of rupture grooves 8 are sequentially disposed along the vertical direction of the struts 6.
  • the plurality of rupture grooves 8 are disposed at equal intervals along the vertical direction of the struts 6.
  • the rupture groove 8 is disposed obliquely, and the open end of the rupture groove 8 is disposed lower than the break point of the rupture groove 8, and is disposed such that the open end of the rupture groove 8 is a contact portion of the struts 6.
  • the struts 6 at both ends of the transverse joint 4 are provided with a rupture groove 8 at the same height position.
  • the struts 6 are divided into multiple sections of the brittle connection by the rupture groove 8, and the corresponding sections of the two struts 6 are provided.
  • the strut 6 can be broken at the position of the rupture groove 8 according to practical needs, so that the struts 6 of the same height are retained at both ends of the traverse 4, thereby adapting to the height of different culture dishes, and adapting to the retention.
  • the tilt compensation mechanism adopts a contour adjustment mechanism, and the contour adjustment mechanism includes a foot pad 17 and a square tube 18 vertically disposed above the rubber pad.
  • the opposite side walls of the square tube 18 are respectively provided with slide plates 19 which slide axially along the square tube 18, and the opposite sides of the slide plate 19 are respectively provided with teeth.
  • a spur gear 20 is disposed between the slide plates 19, and the teeth on both sides of the spur gear 20 are respectively engaged with the teeth of the slide plate 19, and the spur gear 20 is connected to the inner arm of the square tube 18 through the shaft.
  • a first bevel gear 21 is coaxially connected to one end of the spur gear 20, and a reciprocating screw 23 is coaxially disposed at an end of the square pipe 18 at a distance of the spur gear 20 away from the foot pad 17. The reciprocating screw 23 passes through one end of the spur gear 20
  • the second bevel gear 22 meshes with the first bevel gear 21.
  • the side wall surface of the reciprocating screw 23 is provided with a reciprocating groove 24.
  • the square tube 18 is further provided with a telescopic cylinder 31 which is nested between the square tube 18 and the reciprocating screw 23.
  • the inner wall of the telescopic cylinder 31 and the reciprocating screw 23 is provided with a reciprocating joint with the bottom end of the reciprocating groove 24.
  • the top of the telescopic cylinder is connected to the bottom of the pole.
  • the second bevel gear 22 is coaxially connected to the reciprocating screw 23 via a journal 27 .
  • the inner side wall of the square tube 18 which does not overlap with the slide plate 19 is provided with a baffle 26 which cooperates with the shaft hole of the journal 27, and the thickness of the baffle 26 coincides with the length of the journal 27.
  • the contour compensation mechanism enters a highly compensated working state.
  • the foot pad 17 is always attached to the culture dish base or the table top, and the square tube 18 passes.
  • the constraint of the telescopic cylinder 31 and the strut is inclined together with the petri dish pipetting device.
  • the sliding plate 19 slides in the square cylinder, and while the sliding plate 19 slides, the teeth on the side of the sliding plate 19 drive the spur gear 20 to rotate and rotate.
  • the rear spur gear 20 rotates the reciprocating screw 23 by the transmission action of the first bevel gear 21 and the second bevel gear 22. Since the reciprocating screw 23 is provided with the reciprocating groove 24, the reciprocating slider 25 in the telescopic cylinder 31 reciprocates The groove 24 is driven to slide upward and upward, and at the same time, the telescopic cylinder 31 is extended outward, and the height of the strut is compensated. Since the curvature of the reciprocating groove 24 is calculated in relation to the gear ratio of the spur gear 20, the telescopic cylinder 31 is thus obtained.
  • the degree of change can compensate for the change of the height of the strut caused by the tilt, so that the petri dish pipetting device is not placed within a certain range of inclination angle, and the relative height of the nozzle is changed, thereby ensuring that the remaining liquid amount after pipetting reaches the plan. It is required to make the pipetting work simple and efficient.
  • the interior of the contour adjustment mechanism may be modified to have a structure in which the height adjustment mechanism includes a foot pad 17, a square tube 19 vertically disposed above the rubber pad 17, and a square tube 18 opposite to each other.
  • the two side walls are respectively provided with a slide plate 19 which slides along the axial direction of the square tube 18, and the opposite side of the slide plate 19 is provided with a helical tooth having the same inclination and the same modulus.
  • the bottom of the reciprocating screw 23 is coaxially connected with a helical gear 28 that meshes with the helical teeth of the slider 19.
  • the end of the helical gear 28 remote from the reciprocating screw 23 is coaxially connected with a shaft head 29, and the side wall of the shaft head 29 is provided with a circumferential slot.
  • the inner side wall of the square tube 18 that does not coincide with the slide plate 19 is provided with a positioning block 30 that cooperates with the slot of the shaft head 29.
  • the improved height compensation mechanism is directly meshed and driven by the helical teeth of the sliding plate 19 and the helical gears 28 of the reciprocating screw 23, thereby reducing the number of parts, saving manufacturing costs, and reducing the failure rate of the mechanism.
  • the present disclosure further provides a petri dish pipetting device comprising a plurality of pipettes 1 arranged in a vertical direction and arranged in a straight line in a lateral direction, the bottom end of the pipette 1 is provided For the nozzle 2, one end away from the nozzle 2 is the interface 3. It also includes a transverse joint 4 for laterally fixing the pipette 1, and the side walls on either side of the transverse joint 4 are fixedly connected to the central side wall of each of the pipettes 1. Both ends of the transverse joint 4 are connected with struts 6 extending vertically in the direction of the suction nozzle 2 of the pipette 1, and the struts 6 have the same length and shorter than the pipette 1.
  • the struts 6 are shorter than the length of the pipette 1 in the range of 0-50 mm (excluding the case where the struts are as high as the pipettes).
  • the bottom of the strut 6 is fixed to the base of the petri dish, so that the pipette can be extended to the culture based on the fixed support of the strut 6.
  • the inside of the dish is such that the left and right sides of the pipette 1 are equal in height, and the height of the nozzle is uniform. At this time, the pipette 1 can suck out excess liquid in the dish and retain the same amount of the culture solution.
  • the length of the strut 6 depends on the depth of the hole of the petri dish. Since the base of the petri dish has a certain height, when the length of the strut 6 is shorter than the length of the pipette 1, it can be sucked. After the liquid pipe 1 is inserted into the hole of the culture dish, the bottom end of the strut 6 is in contact with the base plate of the culture dish; the function of the brace 6 is to ensure the height of the left and right sides of the pipette 1 to ensure the suction pipe 1 The nozzles are of the same height, so that the excess liquid in the culture dish can be sucked out through the pipette, so that the remaining liquid level is uniform, which ensures the consistency of the experiment.
  • the embodiment provides a petri dish pipetting device comprising a plurality of pipettes arranged in a vertical direction and arranged in a straight line in a lateral direction, the bottom end of the pipette is set as a nozzle, away from One end of the nozzle is an interface; and further comprises a transverse connection of the laterally fixed pipette, the side wall of either side of the transverse connection is fixedly connected with the central side wall of each of the pipettes, or the straw is passed through the center of the transverse connection, And the bottom surface of the transverse connection can be in contact with the upper surface or edge of the culture dish.
  • the suction pipe 1 can be inserted into the hole of the culture dish to ensure multiple suctions.
  • the height of the liquid pipe 1 is uniform, and the function of the transverse connection 4 is to ensure the height of the left and right sides of the pipette 1 to ensure the height of the suction nozzle 1 is uniform, so that the liquid in the culture dish can be redundant through the pipette The liquid is sucked out to make the remaining liquid level uniform, which ensures the consistency of the experiment.
  • the petri dish pipetting device provided by the embodiment of the present disclosure has the same height of the left and right sides of the pipette, and the nozzle height is uniform. During the pipetting process, the pipette sucks out excess liquid in the culture dish, which can The remaining liquid level is consistent, ensuring the consistency of the experiment, achieving the purpose of reducing costs and flexible application.

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Abstract

一种培养皿移液装置，包括沿竖直方向延伸布置并且沿横向直线排列的若干个吸液管(1)，吸液管(1)的底端设为吸嘴(2)，远离吸嘴(2)的一端为接口(3)。还包括横向固定吸液管(1)的横连(4)，横连(4)任意一侧的侧壁与各个吸液管(1)中部侧壁连接固定。横连(4)的两端均连接设有向吸液管(1)吸嘴(2)方向垂直延伸的撑杆(6)，撑杆(6)长度一致，并且略长于吸液管(1)，撑杆(6)底部设有倾斜补偿机构，从而使培养皿移液装置在使用过程不会产生左右倾斜，同时还可以通过倾斜补偿机构对前后倾斜造成高位差进行高度补偿，使移液操作更精确方便。

Description

一种培养皿移液装置
相关申请的交叉引用
本申请要求于2018年02月13日提交中国专利局的申请号为201820257238.6、名称为“培养基换液装置”的中国专利申请的优先权；要求于2018年03月29日提交中国专利局的申请号为201810274228.8、名称为“培养皿移液装置”的中国专利申请的优先权，其全部内容通过引用结合在本申请中。
技术领域
本公开涉及实验设备技术领域，具体涉及一种培养皿移液装置。
背景技术
在细胞培养试验中，液体培养皿换液操作是必然的一个环节，现有的细胞培养换液操作主要为人工换液和工作站换液。人工换液通常是手持装有排状吸液管的排枪对培养皿进行逐排移液，工作站换液可以进行批量快速的移液。
但是，现在技术中的人工换液和工作站换液均存在很多缺陷。
公开内容
本公开的目的在于，提供一种培养皿移液装置，其通过横连设置在吸管两端的可变支架弥补上述技术背景中存在的手持排枪难以控制的精度的技术问题中至少一个问题。本公开提供的装置亦可通过与泵联合使用达到 快速精确的移液效果。
为实现上述目的，培养皿移液装置包括沿竖直方向延伸布置并且沿横向直线排列的若干个吸液管，吸液管的底端设为吸嘴，远离吸嘴的一端为接口。还包括横向固定吸液管的横连，横连任意一侧的侧壁与各个吸液管中部侧壁连接固定。横连的两端均连接设有向吸液管吸嘴方向垂直延伸的撑杆，撑杆长度一致，并且长于吸液管。
优选的，横连包括横向连通吸液管的接口的横管，横管为两端封闭的中空结构。横管设置在各个吸液管靠近接口的一端，并且在对应吸液管的接口一侧设置有与横管内部连通的凸嘴，各个凸嘴与对应的接口连接配合。横管远离吸液管的一侧中部设有与横管内部连通的真空泵口。
优选的，撑杆与横连为一体结构。
优选的，横连及横管或其一与吸液管为一整体结构。
优选的，撑杆与横管为分体结构，撑杆通过连接器与横管两端连接，连接器包括设置在横管两端的插座，以及设置在撑杆一端与插座对应的插头。
优选的，连接器的插座为设置在横管两端并沿横管轴向内凹的圆柱形凹槽，圆柱形凹槽内壁设有沿圆柱形凹槽周向设置的定位沟；插头为设置在撑杆顶端对应圆柱形凹槽的圆柱形凸头，圆柱形凸头四壁周向设有对应定位沟的定位棱。
优选的，横管两端的圆柱形凹槽外沿设置有缺口，圆柱形凸头靠近撑杆一端设置有对应缺口的凸块，撑杆与吸液管平行设定时，凸块与缺口嵌套配合。
优选的，连接器的圆柱形凹槽为竖直方向设置，且槽口垂直朝向吸液管的吸嘴方向；圆柱形凸头沿撑杆轴线方向设置在撑杆的顶端，并与圆柱形凹槽的对应配合。
优选的，横连两端的撑杆在同样高度的位置设有断裂槽。
优选的，断裂槽设置有多个，多个断裂槽沿着撑杆的竖直方向依次设置。
优选的，多个断裂槽沿着撑杆的竖直方向呈等间距设置。
优选的，断裂槽呈倾斜设置，配置成断裂槽的开口端低于断裂槽的断点，配置成使断裂槽的开口端为撑杆的接触部位。
优选的，撑杆包括多段拼接撑杆段，多段拼接撑杆段依次首位连接。
优选的，包括等高调节机构，等高调节机构包括脚垫、竖直设置在胶垫上方的方管；方管内部相对的两侧壁分别设有沿方管轴向滑动的滑板，滑板相对的一侧分别设有齿牙；两片滑板经方管的底端向外延伸，并分别通过铰接轴与胶垫的两侧铰接；滑板之间设有驱动齿轮，驱动齿轮两侧的齿牙分别与滑板的齿牙啮合，驱动齿轮通过轴与方管内臂连接；驱动齿轮的一端同轴连接设有第一伞齿轮，方管内位于驱动齿轮远离脚垫的一端同轴设有往复丝杠，往复丝杠靠近驱动齿轮的一端通过第二伞齿轮与第一伞齿轮啮合；往复丝杠侧壁表面设有往复槽；方管内还设有嵌套设置在方管与往复丝杠之间的伸缩筒，伸缩筒与往复丝杠配合的内壁设有一处与往复槽最底端配合的往复滑块；伸缩筒的顶部与撑杆底部连接设置。
优选的，第二伞齿轮通过轴颈与往复丝杠同轴连接；方管内部不与滑板重合的内侧壁设有与轴颈轴孔配合的挡板，挡板厚度与轴颈长度一致。
优选的，还包括等高调节机构，等高调节机构包括脚垫、竖直设置在胶垫上方的方管；方管内部相对的两侧壁分别设有沿方管轴向滑动的滑板，滑板相对的一侧分别设有斜度及模数相同的斜齿牙；两片滑板经方管的底端向外延伸，并分别通过铰接轴与胶垫的两侧铰接；往复丝杠底部同轴连接设有斜齿轮，斜齿轮与滑板的斜齿牙啮合；斜齿轮远离往复丝杠的一端同轴连接设有轴头，轴头侧壁设有周向开槽；方管内部不与滑板重合的内侧壁设有与轴头的开槽配合的定位块。
本公开提供了一种培养皿移液装置，包括沿竖直方向延伸布置并且沿横向直线排列的若干个吸液管，吸液管的底端设为吸嘴，远离吸嘴的一端为接口；还包括横向固定所述吸液管的横连，横连任意一侧的侧壁与各个吸液管中部侧壁连接固定；横连的两端均连接设有向吸液管的吸嘴方向垂直延伸的撑杆，撑杆长度一致，并且短于吸液管。
优选的，撑杆短于吸液管的长度范围为0-50mm。
本公开提供了一种培养皿移液装置，包括沿竖直方向延伸布置并且沿横向直线排列的若干个吸液管，吸液管的底端设为吸嘴，远离吸嘴的一端为接口；还包括横向固定吸液管的横连，所述横连任意一侧的侧壁与各个所述吸液管中部侧壁连接固定，所述横连的底面能够与培养皿的上表面或边缘接触。
本公开的有益效果：首先相比传统排枪吸液管，本公开通过设置在吸液管两侧的支撑架保持吸液装置左右垂直角度，因此使用中容易掌握平衡，使各个吸嘴处于同一水平高度，因此在移液过程中，可使遗留的液面高度一致，保证了实验的一致性。其中支撑架可以与吸液管为一体设置，同时还可以通过连接机构设定为可拆卸，可更换，可调用不同尺寸高度的 支撑架，从而达到降低成本，灵活应用的目的。
另外，虽然即使通过在吸液管两端设立左右支撑架限定的吸液管的左右倾斜，但由于使用者的视觉、手感因素，在把持中容易使吸液管向前或向后倾斜，为此本公开还通过倾斜补偿机构来补偿吸液管因前后倾斜造成的吸嘴与液面间的垂直距离变化，从而保证移液剩余量与计划剩余相同，拒绝了因吸液管倾斜造成的移液量变化问题，保证了移液精度。
为简化零件结构，本公开还提出通过斜齿轮直接驱动往复丝杠的结构，为此，降低了倾斜补偿机构的制造成本。
附图说明
为了更清楚地说明本公开实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图是本公开的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。
图1为连体撑杆的培养皿移液装置示意图；
图2为分体撑杆的培养皿移液装置示意图；
图3为撑杆连接器示意图；
图4为倾斜补偿机构示意图；
图5为倾斜补偿机构内部剖视图；
图6为倾斜补偿机构改进方案结构示意图；
图7为横连结构示意图；
图8为撑杆连接器定位机构示意图；
图9为撑杆短于吸液管的结构示意图；
图10为横连固定吸液管的结构示意图。
图标：1-吸液管；2-吸嘴；3-接口；32-横管；4-横连；41-单向阀；5-凸嘴；6-撑杆；7-真空泵口；8-断裂槽；11-圆柱形凹槽；12-圆柱形凸头；13-定位沟；14-定位棱；15-缺口；16-凸块；17-脚垫；18-方管；19-滑板；20-直齿轮；21-第一伞齿轮；22-第二伞齿轮；23-往复丝杠；24-往复槽；25-往复滑块；26-挡板；27-轴颈；28-斜齿轮；29-轴头；30-定位块；31-伸缩筒。
具体实施方式
为使本公开实施例的目的、技术方案和优点更加清楚，下面将结合本公开实施例中的附图，对本公开实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例是本公开一部分实施例，而不是全部的实施例。基于本公开中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本公开保护的范围。
在本公开的描述中，需要说明的是，如出现术语“中心”、“上”、“下”、“左”、“右”、“竖直”、“水平”、“内”、“外”等，其所指示的方位或位置关系为基于附图所示的方位或位置关系，仅是为了便于描述本公开和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本公开的限制。此外，如出现术语“第一”、“第二”、“第三”仅配置成描述目的，而不能理解为指示或暗示相对重要性。
在本公开的描述中，需要说明的是，除非另有明确的规定和限定，如出现术语“安装”、“相连”、“连接”应做广义理解，例如，可以是固 定连接，也可以是可拆卸连接，或一体地连接；可以是机械连接，也可以是电连接；可以是直接相连，也可以通过中间媒介间接相连，可以是两个元件内部的连通。对于本领域的普通技术人员而言，可以具体情况理解上述术语在本公开中的具体含义。
在以下的详细描述中，可以参看作为本申请一部分用来说明本申请的特定实施例的各个说明书附图。在附图中，相似的附图标记在不同图式中描述大体上类似的组件。本申请的各个特定实施例在以下进行了足够详细的描述，使得具备本领域相关知识和技术的普通技术人员能够实施本申请的技术方案。应当理解，还可以利用其它实施例或者对本申请的实施例进行结构、逻辑或者电性的改变。
针对现有技术中的人工换液存如下缺点：在手持排枪换液过程中，需平稳的把持排枪，并使排枪吸液口保持在固定的高度，然而由于手的抖动，以及缺乏支撑点等原因，手持排枪过程中，很难使排枪洗液口保持在固定高度，因此导致剩余液量达不到目标要求，而且费时费力，还容易引入污染影响实验结果；
另外，人工换液工作量巨大，费时费力，耗材成本也较高，大量的人工操作增加了污染的概率，一旦出现污染会造成不可估量，甚至无法挽回的损失，同时较难精确控制液体的留存量。
工作站换液虽然能克服人工换液的精度不足，但是其设备开销投入巨大，仪器投入巨大，不适合大面积推广应用，通常耗材成本还比人工换液的耗材成本要高很多。
请参阅图1、图2、图3、图4、图5、图6、图7、图8，包括沿竖直方向延伸布置并且沿横向直线排列的若干个吸液管1，吸液管1的底端设 为吸嘴2，远离吸嘴2的一端为接口3。还包括横向固定吸液管1的横连4，横连4任意一侧的侧壁与各个吸液管1中部侧壁连接固定。横连4的两端均连接设有向吸液管1吸嘴2方向垂直延伸的撑杆6，撑杆6长度一致，并且长于吸液管1。
其中，撑杆6高于吸液管1的长度范围为0-50mm(不包括撑杆与吸液管等高的情况)。
进一步地，横连4包括横向连通吸液管1的接口3的横管32，横管32为两端封闭的中空结构。横管32设置在各个吸液管1靠近接口的一端，并且在对应吸液管1的接口3一侧设置有与横管32内部连通的凸嘴5，各个凸嘴5与对应的接口3连接配合。横管32远离吸液管1的一侧中部设有与横管32内部连通的真空泵口7。
优选地，横连4与吸液管1为一整体结构。
可选地，撑杆6与横管32及横连4可以为一体结构；或者，撑杆6与横管32也可以为分体结构。
工作原理：在使用中，操作者将培养皿移液装置通过真空泵口7连接真空泵，并通过撑杆6与培养皿底座或试验台台面接触，从而使吸液管1的左右两侧等高，吸嘴高度一致。此时吸液管1便可将培养皿内的多余液体吸出，并保留同样多的培养液。其中撑杆6与横连4可以为一体结构，此时换液装置使用起来简单可靠，且两端的撑杆6在制造过程中高度一致精度高，省去了人工调整的步骤。
如图2所示，另外，撑杆6与横连4还可以为可拆卸的分体结构，此时，撑杆6通过连接器与横连4两端连接，连接器包括设置在横连4两端的插座，还包括设置在撑杆6一端与插座对应的插头。其中，连接器的插 座为设置在横连4两端并沿横连4轴向内凹的圆柱形凹槽11，凹槽内壁设有沿圆柱形凹槽11周向设置的定位沟13。撑杆6包括设置在撑杆6顶端对应圆柱形凹槽11的圆柱形凸头12，圆柱形凸头12四壁周向设有对应定位沟13的定位棱14，在使用过程中，可以将不同高度的撑杆6连接在横连4两端，并牢靠的固定，从而不但达到适应培养皿及不同液面高度的需求，分体的撑杆6可反复利用，达到了节省材料的目的。
如图3所示，为了在安装使用时，避免撑杆6在横连4上转动影响工作精度，因此连接器包括设置在横连4两端的圆柱形凹槽11外沿的处的缺口15，还包括设置在圆柱形凸头12靠近撑杆6一端对应缺口15的凸块16，只有在撑杆6与吸液管1平行安装时，凸块16与缺口15嵌套配合，圆柱形凸头12才会顺利的***圆柱形凹槽11中，从而达到角度约束的目的。
为了防止撑杆6在横连两端发生偏转错位，连接器可以按照撑杆6的轴向设定，此时圆柱形凹槽11的槽口垂直朝向吸液管1的吸嘴2方向。圆柱形凸头12沿撑杆6轴线方向设置在撑杆6的顶端，并与圆柱形凹槽的11对应配合。
在以上的撑杆6的设定方式中，撑杆6还可以为多段拼接结构，因此撑杆6根据不同长度设为多组左右成对的形式出现，且每对撑杆6顶部均同轴设有与圆柱形凹槽11对应的圆柱形凸头12，而每对撑杆6的底部同样设有与之对应的圆柱形凹槽11，因此，多段撑杆6可以首尾相接进行长度调整，从而达到在实验要求中灵活运用的效果。
如图8所示，优选的，横连4两端的撑杆6在同样高度的位置设有断裂槽8。
可选地，断裂槽8设置有多个，多个断裂槽8沿着撑杆6的竖直方向依次设置。优选的，多个断裂槽8沿着撑杆6的竖直方向呈等间距设置。
优选的，断裂槽8呈倾斜设置，配置成断裂槽8的开口端低于断裂槽8的断点，配置成使断裂槽8的开口端为撑杆6的接触部位。
可选地，横连4两端的撑杆6在同样高度的位置设有断裂槽8，此时撑杆6被断裂槽8分成脆连接的多段，且两条撑杆6的对应段上均设有对应标记，在实用过程中，可根据实用需要将撑杆6以断裂槽8位置折断，使横连4两端保留连接同样高度的撑杆6，从而达到适应不同培养皿高度，以及适应保留不同高度的液面的需求。由于断裂槽8的断点高于撑杆6最底位置，因此断口不影响撑杆6高度的精确度。
由于上述的撑杆6只能起到防止培养皿移液装置左右倾斜的功能，然而在使用应用过程中，培养皿移液装置很难保持前后方向保持垂直，为此培养皿移液装置包括倾斜补偿机构。如图4-5所示，倾斜补偿机构采用等高调节机构，等高调节机构包括脚垫17、竖直设置在胶垫上方的方管18。方管18内部相对的两侧壁分别设有沿方管18轴向滑动的滑板19，滑板19相对的一侧分别设有齿牙。两片滑板19经方管18的底端向外延伸，并分别通过铰接轴与胶垫的两侧铰接。滑板19之间设有直齿轮20，直齿轮20两侧的齿牙分别与滑板19的齿牙啮合，直齿轮20通过轴与方管18内臂连接。直齿轮20的一端同轴连接设有第一伞齿轮21，方管18内位于直齿轮20远离脚垫17的一端同轴设有往复丝杠23，往复丝杠23靠近直齿轮20的一端通过第二伞齿轮22与第一伞齿轮21啮合。往复丝杠23侧壁表面设有往复槽24。方管18内还设有嵌套设置在方管18与往复丝杠23之间的伸缩筒31，伸缩筒31与往复丝杠23配合的内壁设有一处与往复槽24最 底端配合的往复滑块25。伸缩筒的顶部与撑杆底部连接设置。第二伞齿轮22通过轴颈27与往复丝杠23同轴连接。方管18内部不与滑板19重合的内侧壁设有与轴颈27轴孔配合的挡板26，挡板26厚度与轴颈27长度一致。
当操作者设置好培养皿移液装置的吸嘴高度后，在移液操作时，培养皿移液装置与培养皿底座或桌面产生一个变动或不垂直的夹角，因此导致吸嘴2高度与培养皿底座或桌面的高度变化，此时等高补偿机构进入高度补偿的工作状态，首先，操作者的下压力作用下，脚垫17始终与培养皿底座或桌面贴合，而方管18通过伸缩筒31与撑杆的约束随培养皿移液装置一同倾斜，此时滑板19在方筒内产生相互滑动，在滑板19滑动的同时，滑板19一侧的齿牙带动直齿轮20旋转，旋转后的直齿轮20，通过第一伞齿轮21及第二伞齿轮22的传动作用使往复丝杠23旋转，由于往复丝杠23设有往复槽24，伸缩筒31内的往复滑块25在往复槽24的带动下向上滑动，同时带动伸缩筒31向外伸出，并对撑杆的高度进行补偿，由于往复槽24的曲率与直齿轮20的齿比进行相关计算得出，因此伸缩筒31的长度变化可弥补因倾斜造成的撑杆高度变化，从而使培养皿移液装置在一定范围的倾斜角度内，不置于使吸嘴的相对高度发生改变，因此保证移液后的剩余液量达到计划要求，使移液工作变的简单高效。
另外，如图6所示，等高调节机构内部还可改进为如下结构：在保证等高调节机构包括脚垫17、竖直设置在胶垫17上方的方管19；方管18内部相对的两侧壁分别设有沿方管18轴向滑动的滑板19的基础上，滑板19相对的一侧分别设有斜度及模数相同的斜齿牙。往复丝杠23底部同轴连接设有斜齿轮28，斜齿轮28与滑板19的斜齿牙啮合。斜齿轮28远离往复丝杠23的一端同轴连接设有轴头29，轴头29侧壁设有周向开槽。方 管18内部不与滑板19重合的内侧壁设有与轴头29的开槽配合的定位块30。
改进后的高度补偿机构通过滑板19的斜齿牙与往复丝杠23的斜齿轮28直接啮合驱动，减少了零件数量，节省了制作成本，降低了机构的故障率。
如图9所示，进一步地，本公开还提供了一种培养皿移液装置，包括沿竖直方向延伸布置并且沿横向直线排列的若干个吸液管1，吸液管1的底端设为吸嘴2，远离吸嘴2的一端为接口3。还包括横向固定吸液管1的横连4，横连4任意一侧的侧壁与各个吸液管1中部侧壁连接固定。横连4的两端均连接设有向吸液管1吸嘴2方向垂直延伸的撑杆6，撑杆6长度一致，并且短于吸液管1。
优选的，撑杆6短于吸液管1的长度范围为0-50mm(不包括撑杆与吸液管等高的情况)。
当撑杆6短于吸液管1的长度时，将撑杆6的底部固定于培养皿底座上，从而可以将在撑杆6的固定支撑的基础上，使得吸液管可以伸入至培养皿内部的孔内，从而使吸液管1的左右两侧等高，吸嘴高度一致，此时吸液管1便可将培养皿内的多余液体吸出，并保留同样多的培养液。
本实施例中，撑杆6的长度取决于培养皿的孔的深度，由于培养皿的基座上具有一定的高度，当撑杆6的长度短于吸液管1的长度时，可以在吸液管1伸入培养皿孔内后，将撑杆6的底端与培养皿的底座板接触；撑杆6的作用是保证吸液管1的左右两侧等高，保证吸液管1的吸嘴高度一致，从而可以通过吸液管将培养皿内的多余液体吸出，可使遗留的液面高度一致，保证了实验的一致性。
如图10所示，本实施例提供了一种培养皿移液装置，包括沿竖直方向延伸布置并且沿横向直线排列的若干个吸液管，吸液管的底端设为吸嘴，远离吸嘴的一端为接口；还包括横向固定吸液管的横连，所述横连任意一侧的侧壁与各个所述吸液管中部侧壁连接固定，或者吸管从横连中心穿过，且横连的底面能够与培养皿的上表面或边缘接触。
本实施例中，由于培养皿的基座上具有一定的高度，当将横连4直接放置于培养皿的基座上，可以在吸液管1伸入培养皿孔内后，保证多个吸液管1所处的高度一致，横连4的作用是保证吸液管1的左右两侧等高，保证吸液管1的吸嘴高度一致，从而可以通过吸液管将培养皿内的多余液体吸出，可使遗留的液面高度一致，保证了实验的一致性。
上述实施例仅供说明本公开之用，而并非是对本公开的限制，有关技术领域的普通技术人员，在不脱离本公开范围的情况下，还可以做出各种变化和变型，因此，所有等同的技术方案也应属于本公开公开的范畴。
工业实用性
本公开实施例提供的一种培养皿移液装置，通过吸液管的左右两侧等高，吸嘴高度一致，在移液过程中，吸液管将培养皿内的多余液体吸出，可使遗留的液面高度一致，保证了实验的一致性，达到降低成本，灵活应用的目的。

Claims (20)

  1. 一种培养皿移液装置，其特征在于：包括沿竖直方向延伸布置并且沿横向直线排列的若干个吸液管(1)，所述吸液管(1)的底端设为吸嘴(2)，远离所述吸嘴(2)的一端为接口(3)；还包括横向固定所述吸液管(1)的横连(4)，所述横连(4)任意一侧的侧壁与各个所述的吸液管(1)中部侧壁连接固定；所述横连(4)的两端均连接设有向所述吸液管(1)吸嘴(2)方向垂直延伸的撑杆(6)，所述的撑杆(6)长度一致，并且长于所述吸液管(1)。
  2. 根据权利要求1所述的培养皿移液装置，其特征在于：所述横连(4)包括横向连通所述吸液管(1)的所述接口(3)的横管(32)，所述横管(32)为两端封闭的中空结构；所述横管(32)设置在各个所述吸液管(1)靠近接口的一端，并且在对应所述吸液管(1)的所述接口(3)一侧设置有与所述横管(32)内部连通的凸嘴(5)，各个所述的凸嘴(5)与对应的所述接口(3)连接配合；所述横管(32)远离所述吸液管(1)的一侧中部设有与所述横管(32)内部连通的真空泵口(7)。
  3. 根据权利要求2所述的培养皿移液装置，其特征在于：所述撑杆(6)与所述的横管(32)及所述的横连(4)为一体结构。
  4. 根据权利要求2所述的培养皿移液装置，其特征在于：所述横连(4)与所述吸液管(1)为一整体结构。
  5. 根据权利要求2所述的培养皿移液装置，其特征在于：所述撑杆(6)与所述横管(32)为分体结构，所述撑杆(6)通过连接器与所述横管(32)两端连接，所述连接器包括设置在所述横管(32)两端的插座，以及设置在所述撑杆(6)一端与所述插座对应的插头。
  6. 根据权利要求5所述的培养皿移液装置，其特征在于：所述连接器的所述插座为设置在所述横管(32)两端并沿所述横管(32)轴向内凹的圆柱形凹槽(11)，所述圆柱形凹槽(11)内壁设有沿所述圆柱形凹槽(11)周向设置的定位沟(13)；所述插头为设置在所述撑杆(6)顶端对应所述圆柱形凹槽(11)的圆柱形凸头(12)，所述圆柱形凸头(12)四壁周向设有对应所述定位沟(13)的定位棱(14)。
  7. 根据权利要求6所述的培养皿移液装置，其特征在于：所述横管(32)两端的所述圆柱形凹槽(11)外沿设置有缺口(15)，所述圆柱形凸头(12)靠近所述撑杆(6)一端设置有对应所述缺口(15)的凸块(16)，所述撑杆(6)与所述吸液管(1)平行设定时，所述凸块(16)与所述缺口(15)嵌套配合。
  8. 根据权利要求6所述的培养皿移液装置，其特征在于：所述连接器的所述圆柱形凹槽(11)为竖直方向设置，且槽口垂直朝向所述吸液管(1)的所述吸嘴(2)方向；所述圆柱形凸头(12)沿所述撑杆(6)轴线方向设置在所述撑杆(6)的顶端，并与所述圆柱形凹槽的(11)对应配合。
  9. 根据权利要求1-8任一项所述的培养皿移液装置，其特征在于：所述横连(4)两端的所述撑杆(6)在同样高度的位置设有断裂槽(8)。
  10. 根据权利要求9所述的培养皿移液装置，其特征在于：所述断裂槽(8)设置有多个，多个所述断裂槽(8)沿着所述撑杆(6)的竖直方向依次设置。
  11. 根据权利要求10所述的培养皿移液装置，其特征在于：多个所述断裂槽(8)沿着所述撑杆(6)的竖直方向呈等间距设置。
  12. 根据权利要求9-11所述的培养皿移液装置，其特征在于：所述断 裂槽(8)呈倾斜设置，配置成所述断裂槽(8)的开口端低于所述断裂槽(8)的断点，配置成使所述断裂槽(8)的开口端为所述撑杆(6)的接触部位。
  13. 根据权利要求1-12任一项所述的培养皿移液装置，其特征在于：所述撑杆(6)包括多段拼接撑杆段，多段拼接撑杆段依次首位连接。
  14. 根据权利要求1-13任一项所述的培养皿移液装置，其特征在于：包括等高调节机构，所述等高调节机构包括脚垫(17)、竖直设置在所述胶垫上方的方管(18)；所述方管(18)内部相对的两侧壁分别设有沿所述方管(18)轴向滑动的滑板(19)，所述滑板(19)相对的一侧分别设有齿牙；两片所述滑板(19)经所述方管(18)的底端向外延伸，并分别通过铰接轴与所述胶垫的两侧铰接；所述的滑板(19)之间设有直齿轮(20)，所述直齿轮(20)两侧的齿牙分别与所述滑板(19)的齿牙啮合，所述直齿轮(20)通过轴与所述方管(18)内臂连接；所述直齿轮(20)的一端同轴连接设有第一伞齿轮(21)，所述方管(18)内位于所述直齿轮(20)远离所述脚垫(17)的一端同轴设有往复丝杠(23)，所述往复丝杠(23)靠近所述直齿轮(20)的一端通过第二伞齿轮(22)与所述的第一伞齿轮(21)啮合；所述往复丝杠(23)侧壁表面设有往复槽(24)；所述方管(18)内还设有嵌套设置在所述方管(18)与所述往复丝杠(23)之间的伸缩筒(31)，所述伸缩筒(31)与所述往复丝杠(23)配合的内壁设有一处与所述往复槽(24)最底端配合的往复滑块(25)；所述伸缩筒的顶部与所述撑杆(6)底部连接设置。
  15. 根据权利要求14所述的培养皿移液装置，其特征在于：所述的第二伞齿轮(22)通过轴颈(27)与所述的往复丝杠(23)同轴连接；所述 方管(18)内部不与所述滑板(19)重合的内侧壁设有与所述轴颈(27)轴孔配合的挡板(26)，所述挡板(26)厚度与所述轴颈(27)长度一致。
  16. 根据权利要求1-13任一项所述的培养皿移液装置，其特征在于：还包括等高调节机构，所述等高调节机构包括脚垫(17)、竖直设置在所述胶垫上方的方管(18)；所述方管(18)内部相对的两侧壁分别设有沿所述方管(18)轴向滑动的滑板(19)，所述滑板(19)相对的一侧分别设有斜度及模数相同的斜齿牙；两片所述滑板(19)经所述方管(18)的底端向外延伸，并分别通过铰接轴与所述胶垫的两侧铰接；所述往复丝杠(23)底部同轴连接设有斜齿轮(28)，所述斜齿轮(28)与所述滑板(19)的斜齿牙啮合；所述斜齿轮(28)远离所述往复丝杠(23)的一端同轴连接设有轴头(29)，所述轴头(29)侧壁设有周向开槽；所述方管(18)内部不与所述滑板(19)重合的内侧壁设有与所述轴头(29)的所述开槽配合的定位块(30)。
  17. 根据权利要求1-16任一项所述的培养皿移液装置，其特征在于：所述撑杆(6)高于所述吸液管(1)的长度范围为0-50mm。
  18. 一种培养皿移液装置，其特征在于：包括沿竖直方向延伸布置并且沿横向直线排列的若干个吸液管(1)，所述吸液管(1)的底端设为吸嘴(2)，远离所述吸嘴(2)的一端为接口(3)；还包括横向固定所述吸液管(1)的横连(4)，所述横连(4)任意一侧的侧壁与各个所述的吸液管(1)中部侧壁连接固定；所述横连(4)的两端均连接设有向所述吸液管(1)吸嘴(2)方向垂直延伸的撑杆(6)，所述的撑杆(6)长度一致，并且短于所述吸液管(1)。
  19. 根据权利要18所述的培养皿移液装置，其特征在于：所述撑杆 (6)短于所述吸液管(1)的长度范围为0-50mm。
  20. 一种培养皿移液装置，其特征在于：包括沿竖直方向延伸布置并且沿横向直线排列的若干个吸液管(1)，所述吸液管(1)的底端设为吸嘴(2)，远离所述吸嘴(2)的一端为接口(3)；还包括横向固定所述吸液管(1)的横连(4)，所述横连(4)任意一侧的侧壁与各个所述吸液管(1)中部侧壁连接固定，所述横连(4)的底面能够与培养皿的上表面或边缘接触。
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