WO2018210126A1 - Integrated sampling box structure for sampling - Google Patents

Integrated sampling box structure for sampling Download PDF

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WO2018210126A1
WO2018210126A1 PCT/CN2018/085291 CN2018085291W WO2018210126A1 WO 2018210126 A1 WO2018210126 A1 WO 2018210126A1 CN 2018085291 W CN2018085291 W CN 2018085291W WO 2018210126 A1 WO2018210126 A1 WO 2018210126A1
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sampling
eluent
container
air
box structure
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PCT/CN2018/085291
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王大平
董承智
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上海德具生物科技有限公司
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure

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  • the invention belongs to the technical field of microbial detection, and in particular to a sampling integrated sampling box structure.
  • Airborne transmission is the main route for some pathogens or viruses to spread, and it is the most difficult way to detect and control in the current environment.
  • the SARS virus is mainly found in the nose, sputum and saliva of the source of infection (human or animal) and is transmitted by air droplets and contact. When a patient coughs, sneezes, or even speaks loudly, the virus spreads to the surrounding air with saliva droplets, dust particles, etc., and spreads with the air.
  • the object of the present invention is to provide a sampling integrated sampling box structure to solve the problem of not being able to quickly and accurately detect and monitor pathogenic bacteria or viruses in the air in a public place.
  • a sampling integrated sampling box structure comprising a sampling container, an eluent storage container, a waste liquid tank, an eluent power pump, and a peristaltic pump;
  • the sampling container is connected to a vacuum pump through a pipeline, an inlet of the eluent power pump is connected to the eluent storage container, and an outlet of the eluent power pump is connected to the sampling container;
  • the bottom of the sampling container is respectively connected to the waste liquid tank and the inlet of the peristaltic pump through a pipeline;
  • the waste liquid tank is connected to the vacuum pump through a pipeline;
  • a porous air distributor is disposed in the sampling container, an inlet of the porous air distributor is connected to a pipeline connected to the vacuum pump; and a filter is disposed at a bottom outlet in the sampling container;
  • An adsorption liquid composed of a microbial lysate and an adsorbed particle is disposed in the sampling container, an air outlet of the porous air distributor is lower than a liquid surface of the adsorbent; and the eluent storage container is provided with Eluent.
  • a first electromagnetic valve is disposed on a pipeline between the sampling container and the vacuum pump; and a second electromagnetic valve is disposed on a pipeline between the waste liquid cylinder and the vacuum pump.
  • a check valve is disposed between the peristaltic pump and the filter.
  • the filter is a sand core filter to separate the adsorbed particles from the microorganism lysate.
  • the volume ratio of the microorganism lysate to the adsorbed particles is 10-20:1.
  • the adsorbed particles are DNA adsorbing particles or RNA adsorbing particles.
  • the height-to-diameter ratio of the adsorbent in the sampling container is 6:1-3:1.
  • the eluate is a DNA eluate or an RNA eluate.
  • the eluent power pump is a peristaltic pump.
  • the sampling integrated sampling box structure of the technical scheme is mainly composed of a negative pressure suction device, a microbial lysate, an adsorbed particle and an eluent, and uses a suction device to adsorb air through a negative pressure to a constant gas flow rate per minute.
  • the liquid collection area in the microbial lysate and the adsorbed particles in the microbial lysate, collects bacteria or virions in the air in a small volume of liquid, simultaneously lyses the collected microorganisms, and releases the DNA or RNA of the microorganisms. Adsorption on adsorbed particles, followed by in-line filtration, elution of DNA or RNA with commercially available DNA or RNA eluents for the detection of the number of microorganisms in the air.
  • the sampling integrated sampling box of the technical solution can detect virions with low density in the air and has high sensitivity.
  • FIG. 1 is a schematic diagram of sampling of a sampling integrated sampling box structure of the present invention
  • FIG. 2 is a schematic view showing the structure of the sampling integrated sampling box of the present invention.
  • the present application provides a sampling integrated sampling box structure, as shown in FIG. 1 and FIG. 2, including a sampling container 1, a filter 13, an eluent storage container 6, a waste liquid tank 7, an eluent power pump 5, and a peristaltic motion. Pump 12.
  • the sampling container 1 is connected to the vacuum pump 2 through the pipeline 3, and the sampling container is supplied with a negative pressure by metering, so that the sampling container can suck a set volume of the microbial air from the atmosphere, and the sample container and the vacuum pump are disposed between the sampling container and the vacuum pump.
  • the first solenoid valve 4 controls the sampling of the sampling container.
  • a porous air distributor is arranged in the sampling container, and an inlet of the porous air distributor is connected to the pipeline 3 connected to the vacuum pump; the air outlet of the porous air distributor distributes the drawn air into the adsorption liquid through a plurality of pores.
  • the direct short circuit rushes out of the liquid, and the plurality of pores distribute the bacteria-carrying air into small bubbles, increasing the contact area between the carrier air and the adsorption liquid, and improving the efficiency of adsorbing microorganisms in the air.
  • An adsorption liquid 9 composed of a microorganism lysate and adsorbed particles is disposed in the sampling container, and an air outlet of the porous air distributor is lower than a liquid surface of the adsorption liquid.
  • the volume ratio of the microorganism lysate to the adsorbed particles is 10-20:1.
  • the adsorbed particles are DNA adsorbing particles or RNA adsorbing particles.
  • the height-to-diameter ratio of the adsorption liquid 9 in the sampling container 1 is 3:1.
  • the microbial lysate is phosphate buffered PBS by dissolving NaCl, KCl, Na 2 HPO 4 and KH 2 PO 4 in distilled water, and adjusting the pH of the solution to 7.4 with HCl. Add distilled water to make up to volume, and steam sterilize at 121 ° C for 20 minutes under high pressure, and store in room temperature or 4 ° C refrigerator.
  • a broad-spectrum high-efficiency adsorption liquid composition is added to a 1 ml PBS solution to which a chitosan nanoparticle having a particle size distribution of 10 to 30 mg and a diameter of 10 to 200 nm is added.
  • a particle size distribution of 10-30. mg of a diameter of 10-200 nm is added to a 1 ml PBS solution with influenza virus antibody-modified nanoparticles.
  • the DNA adsorption particles or the RNA adsorption particles may each be a commercially available adsorption resin such as a DNA (RNA) adsorption resin produced by a company such as Shanghai Shenggong or Beijing Quanjin.
  • a DNA (RNA) adsorption resin produced by a company such as Shanghai Shenggong or Beijing Quanjin.
  • the inlet of the eluent power pump 5 is connected to the eluent storage container 6, and the outlet of the eluent power pump is connected to the sampling container.
  • the eluent power pump is also a peristaltic pump.
  • An eluent 10 is disposed in the eluent storage container; the eluent is a DNA eluate or an RNA eluate.
  • the eluent is a commercially available DNA (RNA) eluate, such as DNA (RNA) eluate produced by Shanghai Shenggong or Beijing Jinjinjin.
  • the bottom of the sampling container is connected to the inlets of the waste liquid tank 7 and the peristaltic pump 12 through pipes, and a check valve 11 is disposed between the peristaltic pump and the filter.
  • a filter 13 is provided at the bottom outlet of the sampling container.
  • the filter is a sand core filter to separate the adsorbed particles from the microorganism lysate.
  • the waste liquid cylinder is connected to the vacuum pump through a pipeline; a second electromagnetic valve 8 is disposed on the pipeline between the waste liquid tank and the vacuum pump.
  • the bacterial or virus sampling region, the bacterial or viral lysis region, and the DNA (RNA) extraction region are all the same region, and are all completed in the sampling container.
  • the vacuum pump is started, and the bacteria air enters the adsorption liquid through the air outlet of the pipeline and the porous air distributor. After the set time is left, the vacuum pump is restarted for vacuuming the waste liquid tank, so that the liquid in the sampling container is sucked.
  • the adsorbed particles adsorbed with DNA (RNA) are trapped on the filter medium in the sampling container; the peristaltic pump is actuated to pressurize the eluent storage container, and the elution hydraulic pressure is introduced into the sampling container to adsorb the particles.
  • the DNA (RNA) was eluted and added to the test tube by a peristaltic pump.

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Abstract

An integrated sampling box structure for sampling, comprising a sampling container (1), an eluent reservoir (6), a waste liquid cylinder (7), an eluent power pump (5), and a peristaltic pump (12). The structure relates to the technical field of microbiological detection, and mainly consists of a negative-pressure air suction device, a microorganism lysate, adsorption particles, and an eluent (10). An air extraction device is utilized to adsorb air by means of negative pressure, so that the air flows through a liquid collection area at constant air flow per minute. Under the action of the microorganism lysate and the adsorption particles in the microorganism lysate, bacteria or virus particles in the air are collected in a small-volume liquid, and meanwhile, collected microorganisms are lysed, so that DNA or RNA of the microorganisms is released and adsorbed onto the adsorption particles; afterwards, the DNA or RNA is eluted with a DNA or RNA eluent by means of on-line filtration, for detecting the number of the microorganisms in the air.

Description

一种采样一体化采样盒结构Sampling integrated sampling box structure 技术领域Technical field
本发明属于微生物检测技术领域，特别是指一种采样一体化采样盒结构。The invention belongs to the technical field of microbial detection, and in particular to a sampling integrated sampling box structure.
背景技术Background technique
空气传播是一些致病菌或病毒传播的主要途径，也是目前环境中最难以检测和控制的途径。Airborne transmission is the main route for some pathogens or viruses to spread, and it is the most difficult way to detect and control in the current environment.
从2003年给全球带来巨大冲击的SARS疫情，到2009年影响国内的H1N1和2013年爆发的禽流感疫情，都是可通过空气传播的病毒性疾病。以SARS病毒为例：病毒主要存在于传染源(人或动物)的鼻涕、痰和唾液中，通过空气飞沫和接触传播。患者通过咳嗽、打喷嚏、甚至大声说话时都会将病毒随唾液飞沫、尘埃粒子等扩散到周围的空气中，随空气流动传播。From the SARS epidemic that brought huge impacts to the world in 2003, the H1N1 affecting the country in 2009 and the bird flu outbreak that broke out in 2013 are all airborne viral diseases. Take the SARS virus as an example: the virus is mainly found in the nose, sputum and saliva of the source of infection (human or animal) and is transmitted by air droplets and contact. When a patient coughs, sneezes, or even speaks loudly, the virus spreads to the surrounding air with saliva droplets, dust particles, etc., and spreads with the air.
SARS初期之所以造成大量人员感染特别是医护人员带来巨大牺牲，除防护措施的原因外，很大程度上是由于空气中的病毒难以监测和防范造成的。In the early stage of SARS, a large number of people infected, especially medical personnel, caused great sacrifices. In addition to the protective measures, it was largely caused by the difficulty in monitoring and preventing viruses in the air.
SARS疫情虽然过去十几年了，但它给社会生活带来的巨大影响和社会的恐慌情绪让我们记忆犹新。时至今日，对于空气中有害微生物的预警与监测仍然缺乏有效的工具和方法。现有检测仪器及检测方法程序复杂，耗时较长，难以及时提供疫情现场准确的数据信息以便迅速采取防范措施。Although the SARS epidemic has been over the past decade, its great impact on social life and the panic of society have made us remember. To date, there is still a lack of effective tools and methods for early warning and monitoring of harmful microorganisms in the air. The existing testing instruments and testing methods are complicated and time consuming, and it is difficult to provide timely and accurate data information of the epidemic situation in order to quickly take preventive measures.
随着社会的发展，不同国家、地区人们的交往越来越频繁，因此公共交通工具、交通站点、学校、医院、商场、写字楼等人流密集的封闭区域已经成为空气中传播疾病的主要场所。特别是在医院中，院内交叉感染一直是令医院头疼的问题。With the development of society, people from different countries and regions have become more and more frequent. Therefore, public transport, transportation sites, schools, hospitals, shopping malls, office buildings and other crowded closed areas have become the main places for spreading diseases in the air. Especially in hospitals, cross-infection in hospitals has always been a headache for hospitals.
当禽流感病毒流行时，政府往往在没有直接证据情况下对家禽养殖场进行盲目地扑杀，给政府和养殖场都造成巨大损失。When the avian flu virus is prevalent, the government often blindly culls poultry farms without direct evidence, causing huge losses to the government and farms.
发明内容Summary of the invention
本发明的目的是提供一种采样一体化采样盒结构，以解决不能在公共场所的现场迅速准确的对空气中致病菌或病毒进行预警和监测的问题。The object of the present invention is to provide a sampling integrated sampling box structure to solve the problem of not being able to quickly and accurately detect and monitor pathogenic bacteria or viruses in the air in a public place.
本发明是通过以下技术方案实现的：The invention is achieved by the following technical solutions:
一种采样一体化采样盒结构，包括采样容器、洗脱液存储容器、废液缸、洗脱液动力泵、及蠕动泵；A sampling integrated sampling box structure, comprising a sampling container, an eluent storage container, a waste liquid tank, an eluent power pump, and a peristaltic pump;
所述采样容器通过管路与真空泵连接，所述洗脱液动力泵的入口与所述洗脱液存储容器连接，所述洗脱液动力泵的出口与所述采样容器连接；The sampling container is connected to a vacuum pump through a pipeline, an inlet of the eluent power pump is connected to the eluent storage container, and an outlet of the eluent power pump is connected to the sampling container;
所述采样容器的底部通过管路分别与所述废液缸及所述蠕动泵的入口连接；The bottom of the sampling container is respectively connected to the waste liquid tank and the inlet of the peristaltic pump through a pipeline;
所述废液缸通过管路与所述真空泵连接；The waste liquid tank is connected to the vacuum pump through a pipeline;
在所述采样容器内设置有多孔空气分布器，所述多孔空气分布器的入口与连接真空泵的管路连接；在所述采样容器内的底部出口处设置有过滤器；a porous air distributor is disposed in the sampling container, an inlet of the porous air distributor is connected to a pipeline connected to the vacuum pump; and a filter is disposed at a bottom outlet in the sampling container;
在所述采样容器内设置有由微生物裂解液和吸附粒子组成的吸附液，所述多孔空气分布器的出气口低于所述吸附液的液面；在所述洗脱液存储容器内设置有洗脱液。An adsorption liquid composed of a microbial lysate and an adsorbed particle is disposed in the sampling container, an air outlet of the porous air distributor is lower than a liquid surface of the adsorbent; and the eluent storage container is provided with Eluent.
在所述采样容器与所述真空泵之间的管路上设置有第一电磁阀；在所述废液缸与所述真空泵之间的管路上设置有第二电磁阀。A first electromagnetic valve is disposed on a pipeline between the sampling container and the vacuum pump; and a second electromagnetic valve is disposed on a pipeline between the waste liquid cylinder and the vacuum pump.
在所述蠕动泵与所述过滤器之间设置有单向阀。A check valve is disposed between the peristaltic pump and the filter.
所述过滤器为砂芯过滤器，实现吸附粒子与微生物裂解液的分离。The filter is a sand core filter to separate the adsorbed particles from the microorganism lysate.
在所述吸附液中，所述微生物裂解液与所述吸附粒子的体积比为10-20：1。In the adsorbent, the volume ratio of the microorganism lysate to the adsorbed particles is 10-20:1.
所述吸附粒子为DNA吸附粒子或RNA吸附粒子。The adsorbed particles are DNA adsorbing particles or RNA adsorbing particles.
所述吸附液在所述采样容器中的高径比为6：1-3：1。The height-to-diameter ratio of the adsorbent in the sampling container is 6:1-3:1.
所述洗脱液为DNA洗脱液或RNA洗脱液。The eluate is a DNA eluate or an RNA eluate.
所述洗脱液动力泵为蠕动泵。The eluent power pump is a peristaltic pump.
本发明的有益效果是：The beneficial effects of the invention are:
本技术方案的采样一体化采样盒结构，主要由负压吸气装置、微生物裂解液、吸附粒子及洗脱液组成，利用抽气装置，通过负压吸附空气，以每分钟恒定气流量，经过液体采集区，在微生物裂解液和微生物裂解液内的吸附粒子的作用下，使空气中的细菌或病毒粒子收集在小体积的液体中，同时裂解收集到的微生物，释放微生物的DNA或RNA并吸附在吸附粒子上，然后通过在线过滤，用市售的DNA或RNA洗脱液洗脱DNA或RNA，用于检测空气中的微生物数量。The sampling integrated sampling box structure of the technical scheme is mainly composed of a negative pressure suction device, a microbial lysate, an adsorbed particle and an eluent, and uses a suction device to adsorb air through a negative pressure to a constant gas flow rate per minute. The liquid collection area, in the microbial lysate and the adsorbed particles in the microbial lysate, collects bacteria or virions in the air in a small volume of liquid, simultaneously lyses the collected microorganisms, and releases the DNA or RNA of the microorganisms. Adsorption on adsorbed particles, followed by in-line filtration, elution of DNA or RNA with commercially available DNA or RNA eluents for the detection of the number of microorganisms in the air.
本技术方案的采样一体化采样盒能够检测空气中密度较低的病毒粒子，且灵敏度高。The sampling integrated sampling box of the technical solution can detect virions with low density in the air and has high sensitivity.
附图说明DRAWINGS
图1为本发明采样一体化采样盒结构采样示意图；1 is a schematic diagram of sampling of a sampling integrated sampling box structure of the present invention;
图2为本发明采样一体化采样盒结构工作示意图。2 is a schematic view showing the structure of the sampling integrated sampling box of the present invention.
附图标记说明Description of the reference numerals
1采样容器，2真空泵，3管路，4第一电磁阀，5洗脱液动力泵，6洗脱液存储容器，7废液缸，8第二电磁阀，9吸附液，10洗脱液，11单向阀，12蠕动泵，13过滤器。1 sampling container, 2 vacuum pump, 3 lines, 4 first solenoid valve, 5 eluent power pump, 6 eluent storage container, 7 waste tank, 8 second solenoid valve, 9 adsorbent, 10 eluent , 11 check valves, 12 peristaltic pumps, 13 filters.
具体实施方式detailed description
以下通过实施例来详细说明本发明的技术方案，以下的实施例仅是示例性的，仅能用来解释和说明本发明的技术方案，而不能解释为是对本发明技术方案的限制。The following is a detailed description of the technical solutions of the present invention by way of examples, and the following embodiments are merely illustrative and are not to be construed as limiting the technical solutions of the present invention.
本申请提供一种采样一体化采样盒结构，如图1和图2所示，包括采样容器1、过滤器13、洗脱液存储容器6、废液缸7、洗脱液动力泵5及蠕动泵12。The present application provides a sampling integrated sampling box structure, as shown in FIG. 1 and FIG. 2, including a sampling container 1, a filter 13, an eluent storage container 6, a waste liquid tank 7, an eluent power pump 5, and a peristaltic motion. Pump 12.
采样容器1通过管路3与真空泵2连接，通过真空泵对采样容器提供通过计量的负压，以实现采样容器能够从大气中吸入设定体积的带微生物空气，在采样容器与真空泵之间设置有第一电磁阀4，以控制采样容器的采样。The sampling container 1 is connected to the vacuum pump 2 through the pipeline 3, and the sampling container is supplied with a negative pressure by metering, so that the sampling container can suck a set volume of the microbial air from the atmosphere, and the sample container and the vacuum pump are disposed between the sampling container and the vacuum pump. The first solenoid valve 4 controls the sampling of the sampling container.
在采样容器内设置有多孔空气分布器，多孔空气分布器的入口与连接真空泵的管路3连接；多孔空气分布器的出气口通过多个细孔方式将抽入的带菌空气分布到吸附液中，为了防止气流太大，直接短路冲出液体，多个细孔将带菌空气分布成小气泡，增加带菌空气与吸附液的接触面积，提高吸附空气中微生物的效率。A porous air distributor is arranged in the sampling container, and an inlet of the porous air distributor is connected to the pipeline 3 connected to the vacuum pump; the air outlet of the porous air distributor distributes the drawn air into the adsorption liquid through a plurality of pores. In order to prevent the airflow from being too large, the direct short circuit rushes out of the liquid, and the plurality of pores distribute the bacteria-carrying air into small bubbles, increasing the contact area between the carrier air and the adsorption liquid, and improving the efficiency of adsorbing microorganisms in the air.
在采样容器内设置有由微生物裂解液和吸附粒子组成的吸附液9，多孔空气分布器的出气口低于吸附液的液面。An adsorption liquid 9 composed of a microorganism lysate and adsorbed particles is disposed in the sampling container, and an air outlet of the porous air distributor is lower than a liquid surface of the adsorption liquid.
在吸附液中，微生物裂解液与吸附粒子的体积比为10-20：1。In the adsorption solution, the volume ratio of the microorganism lysate to the adsorbed particles is 10-20:1.
吸附粒子为DNA吸附粒子或RNA吸附粒子。The adsorbed particles are DNA adsorbing particles or RNA adsorbing particles.
吸附液9在采样容器1中的高径比为3：1。The height-to-diameter ratio of the adsorption liquid 9 in the sampling container 1 is 3:1.
在本申请中，微生物裂解液采用磷酸盐缓冲液PBS，配置方法为，将NaCl、KCl、Na 2HPO 4和KH 2PO 4,溶于蒸馏水中，用HCl调节溶液的pH值至7.4，最后加蒸馏水定容即可，并在121℃高压下蒸气灭菌20分钟，保存存于室温或4℃冰箱中。 In the present application, the microbial lysate is phosphate buffered PBS by dissolving NaCl, KCl, Na 2 HPO 4 and KH 2 PO 4 in distilled water, and adjusting the pH of the solution to 7.4 with HCl. Add distilled water to make up to volume, and steam sterilize at 121 ° C for 20 minutes under high pressure, and store in room temperature or 4 ° C refrigerator.
例如，8g NaCl、0.2g KCl、1.44g Na 2HPO 4和0.24g KH 2PO 4,溶于800ml蒸馏水中，用HCl调节溶液的pH值至7.4，最后加蒸馏水定容至1L即可。121℃高压下蒸气灭菌20分钟，保存存于室温或4℃冰箱中。 For example, 8 g of NaCl, 0.2 g of KCl, 1.44 g of Na 2 HPO 4 and 0.24 g of KH 2 PO 4 are dissolved in 800 ml of distilled water, and the pH of the solution is adjusted to 7.4 with HCl, and finally, distilled water is added to a volume of 1 L. Sterilize at 121 ° C for 20 minutes under high pressure and store in room temperature or 4 ° C refrigerator.
在本申请中，广谱高效吸附液组成：1ml PBS溶液中添加粒径分布为10-30毫克直径为10-200nm的壳聚糖纳米粒子。In the present application, a broad-spectrum high-efficiency adsorption liquid composition is added to a 1 ml PBS solution to which a chitosan nanoparticle having a particle size distribution of 10 to 30 mg and a diameter of 10 to 200 nm is added.
在本申请中，如采用特异性吸附液组成，1ml PBS溶液中添加粒径分布为10-30.毫克直径为10-200nm的表面带有流感病毒抗体修饰的纳米粒子。In the present application, if a specific adsorption solution is used, a particle size distribution of 10-30. mg of a diameter of 10-200 nm is added to a 1 ml PBS solution with influenza virus antibody-modified nanoparticles.
在本申请中，DNA吸附粒子或RNA吸附粒子均可以为市售的吸附树脂，如上海生工或北京全式金等公司生产的DNA(RNA)吸附树脂。In the present application, the DNA adsorption particles or the RNA adsorption particles may each be a commercially available adsorption resin such as a DNA (RNA) adsorption resin produced by a company such as Shanghai Shenggong or Beijing Quanjin.
洗脱液动力泵5的入口与洗脱液存储容器6连接，洗脱液动力泵的出口与采样容器连接，在本申请中，洗脱液动力泵也为蠕动泵。在洗脱液存储容器内设置有洗脱液10；洗脱液为DNA洗脱液或RNA洗脱液，在本申请中，洗脱液为市售DNA(RNA)洗脱液即可，如上海生工或北京金式金等公司生产的DNA(RNA)洗脱液。The inlet of the eluent power pump 5 is connected to the eluent storage container 6, and the outlet of the eluent power pump is connected to the sampling container. In the present application, the eluent power pump is also a peristaltic pump. An eluent 10 is disposed in the eluent storage container; the eluent is a DNA eluate or an RNA eluate. In the present application, the eluent is a commercially available DNA (RNA) eluate, such as DNA (RNA) eluate produced by Shanghai Shenggong or Beijing Jinjinjin.
采样容器的底部通过管路分别与废液缸7及蠕动泵12的入口连接；在蠕动泵与过滤器之间设置有单向阀11。在采样容器的底部出口处设置有过滤器13，在本申请中，过滤器为砂芯过滤器，实现吸附粒子与微生物裂解液的分离。The bottom of the sampling container is connected to the inlets of the waste liquid tank 7 and the peristaltic pump 12 through pipes, and a check valve 11 is disposed between the peristaltic pump and the filter. A filter 13 is provided at the bottom outlet of the sampling container. In the present application, the filter is a sand core filter to separate the adsorbed particles from the microorganism lysate.
废液缸通过管路与真空泵连接；在废液缸与真空泵之间的管路上设置有第二电磁阀8。The waste liquid cylinder is connected to the vacuum pump through a pipeline; a second electromagnetic valve 8 is disposed on the pipeline between the waste liquid tank and the vacuum pump.
在本申请的采样一体化采样盒结构中，细菌或病毒采样区域、细菌或病毒裂解区域及DNA(RNA)提取区域均为同一区域，均在采样容器中完成。In the sampling integrated sampling box structure of the present application, the bacterial or virus sampling region, the bacterial or viral lysis region, and the DNA (RNA) extraction region are all the same region, and are all completed in the sampling container.
工作方式为：The way of working is:
启动真空泵，带菌空气通过管路及多孔空气分布器的出气口进入到吸附液中，静置设定时间后，再次启动真空泵，用于对废液缸吸真空，这样采样容器 中的液体被吸入废液缸中，吸附了DNA(RNA)的吸附粒子被截留在采样容器中的过滤介质上；开动蠕动泵为洗脱液存储容器施压，将洗脱液压入到采样容器中，将吸附粒子上的DNA(RNA)洗脱下，通过蠕动泵加入到检测样品的试管中。The vacuum pump is started, and the bacteria air enters the adsorption liquid through the air outlet of the pipeline and the porous air distributor. After the set time is left, the vacuum pump is restarted for vacuuming the waste liquid tank, so that the liquid in the sampling container is sucked. In the waste liquid tank, the adsorbed particles adsorbed with DNA (RNA) are trapped on the filter medium in the sampling container; the peristaltic pump is actuated to pressurize the eluent storage container, and the elution hydraulic pressure is introduced into the sampling container to adsorb the particles. The DNA (RNA) was eluted and added to the test tube by a peristaltic pump.
以上仅是本发明的优选实施方式的描述，应当指出，由于文字表达的有限性，而在客观上存在无限的具体结构，对于本技术领域的普通技术人员来说，在不脱离本发明原理的前提下，还可以做出若干改进和润饰，这些改进和润饰也应视为本发明的保护范围。The above is only a description of a preferred embodiment of the present invention, and it should be noted that due to the finiteness of the literal expression, there is an infinitely specific structure objectively, and those skilled in the art can leave without departing from the principles of the present invention. In the meantime, several improvements and refinements can be made, and these improvements and retouchings should also be considered as protection scope of the present invention.

Claims (9)

  1. 一种采样一体化采样盒结构，其特征在于：包括采样容器、洗脱液存储容器、废液缸、洗脱液动力泵、及蠕动泵；A sampling integrated sampling box structure, comprising: a sampling container, an eluent storage container, a waste liquid tank, an eluent power pump, and a peristaltic pump;
    所述采样容器通过管路与真空泵连接，所述洗脱液动力泵的入口与所述洗脱液存储容器连接，所述洗脱液动力泵的出口与所述采样容器连接；The sampling container is connected to a vacuum pump through a pipeline, an inlet of the eluent power pump is connected to the eluent storage container, and an outlet of the eluent power pump is connected to the sampling container;
    所述采样容器的底部通过管路分别与所述废液缸及所述蠕动泵的入口连接；The bottom of the sampling container is respectively connected to the waste liquid tank and the inlet of the peristaltic pump through a pipeline;
    所述废液缸通过管路与所述真空泵连接；The waste liquid tank is connected to the vacuum pump through a pipeline;
    在所述采样容器内设置有多孔空气分布器，所述多孔空气分布器的入口与连接真空泵的管路连接；在所述采样容器内的底部出口处设置有过滤器；a porous air distributor is disposed in the sampling container, an inlet of the porous air distributor is connected to a pipeline connected to the vacuum pump; and a filter is disposed at a bottom outlet in the sampling container;
    在所述采样容器内设置有由微生物裂解液和吸附粒子组成的吸附液，所述多孔空气分布器的出气口低于所述吸附液的液面；在所述洗脱液存储容器内设置有洗脱液。An adsorption liquid composed of a microbial lysate and an adsorbed particle is disposed in the sampling container, an air outlet of the porous air distributor is lower than a liquid surface of the adsorbent; and the eluent storage container is provided with Eluent.
  2. 根据权利要求1所述的采样一体化采样盒结构，其特征在于：在所述采样容器与所述真空泵之间的管路上设置有第一电磁阀；在所述废液缸与所述真空泵之间的管路上设置有第二电磁阀。The sampling integrated sampling box structure according to claim 1, wherein a first electromagnetic valve is disposed on a pipeline between the sampling container and the vacuum pump; and the waste liquid cylinder and the vacuum pump A second solenoid valve is disposed between the lines.
  3. 根据权利要求1所述的采样一体化采样盒结构，其特征在于在所述蠕动泵与所述过滤器之间设置有单向阀。The sampling integrated sampling box structure according to claim 1, wherein a check valve is disposed between the peristaltic pump and the filter.
  4. 根据权利要求1所述的采样一体化采样盒结构，其特征在于所述过滤器为砂芯过滤器，实现吸附粒子与微生物裂解液的分离。The sampling integrated sampling box structure according to claim 1, wherein the filter is a sand core filter for separating the adsorbed particles from the microorganism lysate.
  5. 根据权利要求1所述的采样一体化采样盒结构，其特征在于在所述吸附液中，所述微生物裂解液与所述吸附粒子的体积比为10-20：1。The sampling integrated sampling box structure according to claim 1, wherein a volume ratio of said microorganism lysate to said adsorbed particles in said adsorbent is 10-20:1.
  6. 根据权利要求1或5所述的采样一体化采样盒结构，其特征在于所述吸附粒子为DNA吸附粒子或RNA吸附粒子。The sampling integrated sampling box structure according to claim 1 or 5, wherein the adsorbed particles are DNA adsorbing particles or RNA adsorbing particles.
  7. 根据权利要求1所述的采样一体化采样盒结构，其特征在于所述吸附液在所述采样容器中的高径比为6：1-3：1。The sampling integrated sampling box structure according to claim 1, wherein the adsorption liquid has an aspect ratio in the sampling container of 6:1 to 3:1.
  8. 根据权利要求1所述的采样一体化采样盒结构，其特征在于所述洗脱液为DNA洗脱液或RNA洗脱液。The sampling integrated sampling cassette structure according to claim 1, wherein the eluent is a DNA eluate or an RNA eluate.
  9. 根据权利要求1所述的采样一体化采样盒结构，其特征在于所述洗脱 液动力泵为蠕动泵。The sampling integrated sampling cartridge structure according to claim 1, wherein said eluent power pump is a peristaltic pump.
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