WO2017198455A2 - Procédé pour accroître le rendement de plantes bêta spp. - Google Patents

Procédé pour accroître le rendement de plantes bêta spp. Download PDF

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WO2017198455A2
WO2017198455A2 PCT/EP2017/060425 EP2017060425W WO2017198455A2 WO 2017198455 A2 WO2017198455 A2 WO 2017198455A2 EP 2017060425 W EP2017060425 W EP 2017060425W WO 2017198455 A2 WO2017198455 A2 WO 2017198455A2
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mixtures
methyl
plants
plant
seed
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PCT/EP2017/060425
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WO2017198455A3 (fr
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Jan Desomer
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Bayer Cropscience Nv
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A01H3/04—Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Definitions

  • the invention relates to a method for increasing yield in Beta spp. plants (preferably Beta vulgaris plants, in particular sugar beet) comprising at least one event or allele as described, wherein the Beta spp. plants (preferably Beta vulgaris plants, in particular sugar beet), plant parts thereof, plant propagation material or the soil in which Beta spp. plants (preferably Beta vulgaris plants, in particular sugar beet) are grown or intended to be grown are treated with the active ingredients or mixtures thereof.
  • Beta spp. plants preferably Beta vulgaris plants, in particular sugar beet
  • Beta vulgaris (beet) is a plant in the Amaranthaceae family (which is now included in Betoideae subfamily) has numerous cultivated varieties, the most well known of which is the root vegetable known as the beetroot or garden beet. Other cultivated varieties include the leaf vegetable chard; the sugar beet, used to produce table sugar; and whiurzel, which is a fodder crop.
  • Beta spp. plants In view of the nutritional and commercial relevance of Beta spp. plants, further improved methods suitable for increasing yield in Beta spp. plants, preferably in Beta vulgaris plants, in particular sugar beet are desired.
  • Beta spp. plants preferably in Beta vulgaris plants, in particular sugar beet.
  • Beta spp. in particular refers to Beta vulgaris, especially to sugar beet.
  • plants used and referred to in the context of the present invention are Beta spp. plants, preferably Beta vulgaris plants, in particular sugar beet plants.
  • Beta spp. plants preferably in Beta vulgaris plants, in particular sugar beet plants
  • parts thereof, including seeds to increase yield:
  • Herbicidally active compounds useful in the context of the present invention are, for example, known active compounds as they are described in, for example, Weed Research 26, 441-445 (1986), or "The Pesticide Manual”, 16th edition, November 2012, The British Crop Protection Council and the Royal Soc.
  • active compounds which may be mentioned as herbicides or plant growth regulators which are known from the literature and which can be combined with the compounds according to the invention are the following (compounds are either described by "common name” in accordance with the International Organization for Standardization (ISO) or by chemical name or by a customary code number), and always comprise all applicable forms such as acids, salts, ester, or modifications such as isomers, like stereoisomers and optical isomers. As an example at least one applicable form and/or modifications can be mentioned.
  • herbicides useful in the context of the present invention are disclosed in group H-l :
  • O-ethyl isopropylphosphoramidothioate halauxifen, halauxifen-methyl, halosafen, halosulfuron, halosulfuron-methyl, haloxyfop, haloxyfop-P, haloxyfop-ethoxyethyl, haloxyfop-P-ethoxyethyl, haloxyfop-methyl, haloxyfop-P-methyl, hexazinone, HW-02, i.e.
  • Group H-2 discloses herbicides especially used in the context of the present invention are selected from the group consisting of:
  • Group H-3 discloses preferred herbicides are selected from the group consisting of:
  • Glyphosate salts are used as herbicide, preference is given to Glyphosate isopropylamine salt, Glyphosate potassium salt, Glyphosate sodium salt, Glyphosate trimethylsulfonium salt.
  • Iodosulfuron esters and/or salts thereof are used as herbicide, preference is given to Iodosulfuron- methyl and Iodosulfuron-methyl-sodium.
  • Mesosulfuron esters and/or salts thereof are used as herbicide, preference is given to Mesosulfuron- methyl and Mesosulfuron-methyl-sodium. If Thiencarbazone esters and/or salts thereof are used as herbicide, preference is given to Thiencarbazone- methyl and Thiencarbazone-methyl-sodium.
  • Preferred mixtures of herbicides used in the context of the present invention are disclosed in group H-4: mixtures of Acetochlor and other herbicides mentioned herein, mixtures of Dicamba, esters and/or salts thereof and other herbicides mentioned herein, mixtures of Diflufenican and other herbicides mentioned herein, mixtures of Flufenacet and other herbicides mentioned herein, mixtures of Glufosinate and/or salts thereof (preferably Glufosinate- ammonium salt, Glufosinate potassium salt and Glufosinate sodium salt) and other herbicides mentioned herein, mixtures of L-Glufosinate and/or salts thereof (preferably L- Glufosinate-ammonium salt, L-Glufosinate potassium salt and L-Glufosinate sodium salt) and other herbicides mentioned herein, mixtures of Indaziflam and other herbicides mentioned herein, mixtures of Isoxaflutole and other herbicides mentioned herein, mixtures
  • mixtures of herbicides used in the context of the present invention are disclosed in group H-5: mixtures of Acetochlor and Isoxaflutole, mixtures of Acetochlor and Tembotrione mixtures of Diflufenican and Flufenacet, mixtures of Diflufenican and Metribuzin, mixtures of Glufosinate and/or salts thereof and Indaziflam, in particular mixtures of Glufosinate-ammonium and Indaziflam, mixtures of L-Glufosinate and/or salts thereof and Indaziflam, in particular mixtures of L-Glufosinate-ammonium and Indaziflam, mixtures of Mesosulfuron-methyl(-sodium) and Iodosulfuron-methyl(-sodium), mixtures of Mesosulfuron-methyl(-sodium), Iodosulfuron-methyl(-sodium) and Flufenacet, mixtures of Tembotrione and
  • Sl compounds of the type of dichlorophenylpyrazoline-3-carboxylic acid (Sl ), preferably compounds such as l-(2,4-dichlorophenyl)-5-(ethoxycarbonyl)-5-methyl-2-pyrazoline-3-carboxylic acid, ethyl l-(2,4-dichlorophenyl)-5-(ethoxycarbonyl)-5-methyl-2-pyrazoline-3-carboxylate (Sl-1) ("mefenpyr(-diethyl)"), and related compounds, as described in WO-A-91/07874;
  • Sl b derivatives of dichlorophenylpyrazolecarboxylic acid (Sl b ), preferably compounds such as ethyl l-(2,4-dichlorophenyl)-5-methylpyrazole-3-carboxylate (Sl-2), ethyl 1 -(2,4-dichlorophenyl)-5-isopropylpyrazole-3-carboxylate (S 1 -3), ethyl l-(2,4-dichlorophenyl)-5-(l,l-dimethylethyl)pyrazole-3-carboxylate (Sl-4) and related compounds, as described in EP-A-333 131 and EP-A-269 806;
  • Sl c derivatives of l,5-diphenylpyrazole-3-carboxylic acid (Sl c ), preferably compounds such as ethyl l-(2,4-dichlorophenyl)-5-phenylpyrazole-3-carboxylate (Sl-5), methyl l-(2-chlorophenyl)-5-phenylpyrazole-3-carboxylate (Sl-6) and related compounds, as described, for example, in EP-A-268554; Sl d ) compounds of the type of triazolecarboxylic acids (Sl d ), preferably compounds such as fenchlorazole(-ethyl), i.e.
  • S2 compounds of the type of 8-quinolinoxyacetic acid (S2 ), preferably 1-methylhexyl (5-chloro-8-quinolinoxy)acetate (common name "cloquintocet-mexyl” (S2-1), 1,3-dimethyl-but-l-yl (5-chloro-8-quinolinoxy)acetate (S2-2),
  • S2 b compounds of the type of (5-chloro-8-quinolinoxy)malonic acid (S2 b ), preferably compounds such as diethyl (5-chloro-8-quinolinoxy)malonate, diallyl (5-chloro-8-quinolinoxy)malonate, methyl ethyl (5-chloro-8-quinolinoxy)malonate and related compounds, as described in EP-A-0 582 198;
  • S3 Active compounds of the type of dichloroacetamides (S3) which are frequently used as pre- emergence safeners (soil-acting safeners), such as, for example, "dichlormid” (N,N-diallyl-2,2-dichloroacetamide) (S3-1),
  • R-29148 (3-dichloroacetyl-2,2,5-trimethyl-l,3-oxazolidine) from Stauffer (S3-2)
  • R-28725" (3-dichloroacetyl-2,2-dimethyl-l,3-oxazolidine) from Stauffer (S3-3)
  • benoxacor (4-dichloroacetyl-3,4-dihydro-3-methyl-2H-l,4-benzoxazine) (S3-4),
  • PPG-1292 N-allyl-N-[(l,3-dioxolan-2-yl)methyl]dichloroacetamide
  • R A is (Ci-Ce)-alkyl, (C3-C6)-cycloalkyl, where the 2 last-mentioned radicals are substituted by V A substituents from the group consisting of halogen, (Ci-C -alkoxy, halo-(Ci-Ce)- alkoxy and (Ci-C -alkylfhio and, in the case of cyclic radicals, also (Ci-C -alkyl and (Ci-C 4 )-haloalkyl;
  • R A 2 is halogen, (G-C 4 )-alkyl, (Ci-C 4 )-alkoxy, CF 3; iriA is 1 or 2;
  • VD is 0, 1, 2 or 3; compounds of the type of 4-(benzoylsulphamoyl)benzamides of the formula (S4 b ) and salts thereof, as described in WO-A-99/16744,
  • RB 1 , RB 2 independently of one another are hydrogen, (Ci-Ce)-alkyl, (C3-C6)-cycloalkyl, (C3-C 6 )-alkenyl, (C 3 -C 6 )-alkynyl, RB 3 is halogen, (Ci-C -alkyl, (C1-C4) -halo alky 1 or (Ci-C4)-alkoxy, me is 1 or 2; for example those in which
  • RB 1 cyclopropyl
  • RB 2 hydrogen
  • (RB 3 ) 2-OMe
  • RB 1 cyclopropyl
  • RB 2 hydrogen
  • (RB 3 ) 5-Cl-2-OMe (S4-2)
  • RB 1 isopropyl
  • R B 2 hydrogen
  • (RB 3 ) 5-Cl-2-OMe (S4-4) and
  • Rc 1 , Rc 2 independently of one another are hydrogen, (Ci-Cs)-alkyl, (C3-C8)-cycloalkyl, (C3-C6)- alkenyl, (C3-Ce)-alkynyl,
  • Rc 3 is halogen, (Ci-C 4 )-alkyl, (Ci-C 4 )-alkoxy, CF 3 , mc is 1 or 2; for example l-[4-(N-2-methoxybenzoylsulphamoyl)phenyl]-3-methylurea,
  • S4 d compounds of the type of N-phenylsulphonylterephthalamides of the formula (S4 d ) and salts thereof, which are known, for example, from CN 101838227, in which
  • RD 4 is halogen, (Ci-C 4 )-alkyl, (Ci-C 4 )-alkoxy, CF 3 ; mo is 1 or 2;
  • RD 5 is hydrogen, (Ci-C 6 )-alkyl, (C 3 -C 6 )-cycloalkyl, (C 2 -C 6 )-alkenyl, (C 2 -C 6 )-alkynyl, (C 5 -C 6 )- cycloalkenyl;
  • Active compounds from the class of hydroxyaromatics and aromatic-aliphatic carboxylic acid derivatives (S5) for example ethyl 3,4,5-triacetoxybenzoate, 3,5-dimethoxy-4-hydroxybenzoic acid, 3,5-dihydroxybenzoic acid, 4-hydroxysalicylic acid, 4-fluorosalicyclic acid, 2-hydroxycinnamic acid, 2,4- dichlorocinnamic acid, as described in WO-A-2004/084631, WO-A-2005/015994, WO-A- 2005/016001;
  • Active compounds from the class of l,2-dihydroquinoxalin-2-ones for example 1 -methyl-3-(2-thienyl)- l,2-dihydroquinoxalin-2-one, 1 -methyl-3-(2-thienyl)- 1 ,2- dihydroquinoxaline-2-thione, 1 -(2-aminoethyl)-3-(2-thienyl)- 1 ,2-dihydroquinoxalin-2-one hydrochloride, l-(2-methylsulphonylaminoethyl)-3-(2-thienyl)-l,2-dihydroquinoxalin-2-one, as described in WO-A-2005/112630;
  • RD 1 is halogen, (Ci-C -alkyl, (Ci-C -haloalkyl, (Ci-C4)-alkoxy, (Ci-C -haloalkoxy, RD 2 is hydrogen or (Ci-C -alkyl, RD 3 is hydrogen, (Ci-Cs)-alkyl, (C 2 -C4)-alkenyl, (C 2 -C4)-alkynyl or aryl, where each of the carbon-containing radicals mentioned above is unsubstituted or substituted by one or more, preferably by up to three, identical or different radicals from the group consisting of halogen and alkoxy; or salts thereof, nD is an integer from 0 to 2;
  • S9 Active compounds from the class of 3-(5-tetrazolylcarbonyl)-2-quinolones (S9), for example l,2-dihydro-4-hydroxy-l-ethyl-3-(5-tetrazolylcarbonyl)-2-quinolone (CAS Reg. No.: 219479-18- 2), l,2-dihydro-4-hydroxy-l-methyl-3-(5-tetrazolylcarbonyl)-2-quinolone (CAS Reg.
  • RE 1 is halogen, (Ci-C -alkyl, methoxy, nitro, cyano, CF 3 , OCF 3 YE, ZE independently of one another are O or S, nE is an integer from 0 to 4,
  • RE 2 is (Ci-Ci6)-alkyl, (C 2 -Ce)-alkenyl, (C 3 -Ce)-cycloalkyl, aryl; benzyl, halobenzyl, RE 3 is hydrogen or (Ci-Ce)-alkyl;
  • S l l Active compounds of the type of oxyimino compounds (S l l), which are known as seed dressings, such as, for example,
  • Active compounds from the class of isothiochromanones such as, for example, methyl [(3- oxo- lH-2-benzothiopyran-4(3H)-ylidene)methoxy] acetate (CAS Reg. No.: 205121-04-6) (S 12-1) and related compounds from WO- A- 1998/13361;
  • naphthalic anhydrid (1,8-naphthalenedicarboxylic anhydride) (S 13-1), which is known as seed dressing safener for corn against thiocarbamate herbicide damage,
  • flurazole (benzyl 2-chloro-4-trifluoromethyl-l,3-thiazole-5-carboxylate) (S 13-3), which is known as seed dressing safener for millet against alachlor and metolachlor damage,
  • MG 191 (CAS Reg. No.: 96420-72-3) (2-dichloromethyl-2-methyl-l,3-dioxolane) (S 13-5) from Nitrokemia, which is known as safener for corn,
  • Active compounds which, besides a herbicidal effect against harmful plants, also have a safener effect on crop plants such as rice, such as, for example, "dimepiperate” or "MY 93" (S-l -methyl- 1-phenylethyl piperidine-l-carbothioate), which is known as safener for rice against molinate herbicide damage,
  • RH is (Ci-C 6 )-haloalkyl
  • RH 2 is hydrogen or halogen, independently of one another are hydrogen, (Ci-Ci6)-alkyl, (C 2 -Ci6)-alkenyl or (C 2 -C 16 )-alkynyl, where each of the 3 last-mentioned radicals is unsubstituted or substituted by one or more radicals from the group consisting of halogen, hydroxy, cyano, (Ci-C -alkoxy, (C1-C4)- haloalkoxy, (Ci-C 4 )-alkylthio, (Ci-C 4 )-alkylamino, di-[(Ci-C 4 )-alkyl]-amino, [(C 1 -C 4 )- alkoxy]-carbonyl, [(Ci-C 4 )-haloalkoxy]-carbonyl, unsubstituted or substituted (C 3 -C6)- cycloalkyl, unsubstituted or substitute
  • RH 3 is (Ci-C -alkoxy, (C 2 -C4)-alkenyloxy, (C 2 -Ce)-alkynyloxy or (C 2 -C4)-haloalkoxy, and
  • RH 4 is hydrogen or (Ci-C -alkyl, or RH 3 and RH 4 together with the directly bound N-atom are a 4 to 8-membered heterocyclic ring, which can contain further hetero ring atoms besides the N-atom, preferably up to two further hetero ring atoms from the group consisting of N, O and S, and which is unsubstituted or substituted by one or more radicals from the group consisting of halogen, cyano, nitro, (Ci-C -alkyl, (C 1 -C4)- haloalkyl, (Ci-C4)-alkoxy, (Ci-C4)-haloalkoxy, and (Ci-C4)-alkylthio; S 16) Active compounds which are primarily used as herbicides, but also have a safener effect on crop plants, for example
  • Preferred safeners in the context of the present invention are disclosed in group SAF-2: cloquintocet- mexyl, cyprosulfamid, fenchlorazole-ethyl, isoxadifen-ethyl, mefenpyr-diethyl, fenclorim, cumyluron, S4-1, and S4-5, wherein particular preference is given to the safeners cloquintocet-mexyl, cyprosulfamid, isoxadifen-ethyl, and mefenpyr-diethyl.
  • Particularly preferred safeners used in the context of the present invention are disclosed in group SAF-3: Isoxadifen and its esters, in particular Isoxadifen-ethyl, and Cyprosulfamide.
  • the safeners are preferably used together with one or more of the herbicides mentioned above.
  • Preferred in the context of the present invention are mixtures of safeners disclosed in group SAF-4: mixtures of Isoxadifen-ethyl and the herbicides mentioned herein and mixtures of Cyprosulfamide and the herbicides mentioned herein.
  • herbicides and safeners mentioned above are known, see e.g. "The Pesticide Manual”, 16th edition, November 2012, The British Crop Protection Council and the Royal Soc. of Chemistry.
  • the herbicides and the mixtures of herbicides (and optionally safeners) mentioned herein may be used in pre-emergence applications and/or in post-emergence applications.
  • the herbicides and the mixtures of herbicides (and optionally safeners) mentioned herein may be applied as a split application over time.
  • Another possibility is the application of the individual active ingredients or the mixtures comprising the active ingredients in a plurality of portions (sequential application), for example after pre-emergence applications, followed by post-emergence applications or after early post-emergence applications, followed by applications at medium or late post-emergence.
  • Biologies as used in the context of the present invention are selected from the group B-l consisting of:
  • Bacillus cereus EIP-N2 (Accession No. CNCM 1-1562), Myrothecium verrucaria in particular strain AARC-0255, Flavobacterium sp strain H492 (Accession No. B-50584), Bacillus subtilis AQ713 (Accession No. B-21661), Bacillus subtilis AQ30002 (Accession No. B-50421), Bacillus subtilis AQ 30004 (Accession No. B-50455), Bacilus firmus, especially Bacillus firmus GB 126 (Accession No. CNCM 1-1582) and Bacillus firmus strain 1-1582 (Accession No. CNCMI-1582), Paecilomyces lilacinus 251 (Accession No.
  • DSM 23289 Coniothyrium minitans (Accession No. DSM 9660), Bacillus pumilus QST 2808 (Accession No. NRRL B-30087), Bacillus pumilus GB34 (Accession No. ATCC 700814), Metarhizium anisopliae (Accession No. DSM 3884), Metarhizium anisopliae (Accession No. ATCC 90448), Bacillus subtilis/amyloliquefaciens MBI600 (Accession No. NRRL B-50595), MBI600 + pyraclostrobin), Paenibacillus polymyxa strains Lu 16774 (Accession No.
  • DSM 26969 Lul7007 (Accession No. DSM 26970), and Lul7015 (Accession No. DSM 26971), Bacillus subtilis FZB24 (EPA Registration Number: 72098-5 and EPA Establishment Number: 73386-DEU-001, a strain that is commercially available from Earth Bioscience, Inc.), Pasteuria usage strains (e.g., used in ECONEM), Trichoderma virens GI-3 (Accession No. ATCC 58678) , Bacillus amyloliquefaciens TJ1000 (Accession No.
  • ATCC BAA-390 ATCC BAA-390
  • Bacillus amyloliquefaciens D747 (Accession No. FERM BP-8234), Bradyrhizobium japonicum, Pseudomonas fluorescens, Pseudomonas chloropsis, Rhizobium spp, especially Rhizobium tropici SP25, a recombinant exosporium- producing Bacillus thuringienses cell (especially B. thuringiensis BT013A) that expresses a fusion protein comprising: 1) an endoglucanase, (especially from Bacillus subtilis) having the amino acid sequence of
  • a recombinant exosporium-producing Bacillus thuringiensis cell that expresses a fusion protein comprising: 1) a phosphoplipase having the amino acid sequence of Sequence 3, as herein defined and
  • targeting sequence refers to a polypeptide sequence that results in the localization of a longer polypeptide or the protein to the exosporium of a Bacillus cereus family member.
  • BclA the major constituent of the surface nap, has been shown to be attached to the exosporium with its amino-terminus (N-terminus) positioned at the basal layer and its carboxy-terminus (C- terminus) extending outward from the spore. It was previously discovered that certain sequences from the N- terminal regions of BclA and BclB could be used to target a peptide or protein to the exosporium of a Bacillus cereus endospore (see, e.g., US Patent Publication Nos.
  • a sequence alignment of amino acids 1-41 of BclA (Sequence 1 as herein defined) with the corresponding N-terminal regions of several other Bacillus cereus family exosporium proteins and Bacillus cereus family proteins having related sequences is shown in WO 2016/044529 as Fig. 1. There is a region of high-homology among all of the proteins in the region corresponding to amino acids 20 ⁇ 11 of BclA. However, in these sequences, the amino acids corresponding to amino acids 36-41 of BclA contain secondary structure and are not necessary for fusion protein localization to the exosporium.
  • Sequence 1 is identical with SEQ ID NO.: 1 of WO 2016/044529 referring to the amino acid sequence of AA 1-41 of BclA (herein incorporated by reference)
  • Sequence 2 is identical with SEQ ID NO.: 107 of WO 2016/044529 referring to the amino acid sequence of endonuclease (herein incorporated by reference)
  • Sequence 3 is identical with SEQ ID NO.: 108 of WO 2016/044529 referring to the amino acid sequence of B. thuringiensis BT013A phospholipase C (herein incorporated by reference)
  • Biologies are selected from the group B-2 consisting of:
  • Bacillus subtilis/amyloliquefaciens MB 1600 Paenibacillus polymyxa strains Lu 16774, Lul7007, Lul7015, Bacillus subtilis FZB24, Pasteuria usage strains (e.g. used in ECONEM), Trichoderma virens GI-3, Bacillus amyloliquefaciens TJIOOO, a combination of GI-3 with TJIOOO or FZB24, Bacillus amyloliquefaciens D747, a recombinant exosporium-producing Bacillus thuringienses cell (especially B. thuringiensis BT013A) that expresses a fusion protein comprising:
  • Biologies are selected from the group B-3 consisting of:
  • Biologies are selected from a group B-4 consisting of:
  • Metarhizium anisopliae (Accession No. DSM 3884), Metarhizium anisopliae (Accession No. ATCC 90448), Bacillus subtilis/amyloliquefaciens MBI600 (Accession No. NRRL B-50595), Paenibacillus polymyxa strains Lul6774, Lul7007, Lul7015, Bacillus subtilis FZB24, Pasteuria usage strains (e.g. used in ECONEM), Trichoderma virens GI-3, Bacillus amyloliquefaciens TJ1000, a combination of GI-3 with TJ1000 or FZB24) and Bacillus amyloliquefaciens D747.
  • Biologies are selected from the group B-5 consisting of:
  • Bacillus subtilis AQ713 Bacilus firmus GB 126, Paecilomyces lilacinus strain 251, Coniothyrium minitans (Accession No. DSM 9660), Bacillus pumilus GB34, and Bacillus pumilus QST 2808.
  • Insecticides and Nematicides identified herein by their common names are known and are described, for example, in the pesticide handbook ("The Pesticide Manual” 16th Ed., British Crop Protection Council 2012) or can be found on the Internet (e.g. http://www.alanwood.net/pesticides). The classification is based on the current IRAC Mode of Action Classification Scheme at the time of filing of this patent application.
  • Insecticides used in the context of the present invention are disclosed in the group I-l comprising 29 subgroups:
  • Acetylcholinesterase (AChE) inhibitors such as, for example, carbamates, for example alanycarb, aldicarb, bendiocarb, benfuracarb, butocarboxim, butoxycarboxim, carbaryl, carbofuran, carbosulfan, ethiofencarb, fenobucarb, formetanate, furathiocarb, isoprocarb, methiocarb, methomyl, metolcarb, oxamyl, pirimicarb, propoxur, thiodicarb, thiofanox, triazamate, trimethacarb, XMC and xylylcarb; or organophosphates, for example acephate, azamethiphos, azinphos-ethyl, azinphos-methyl, cadusafos, chlorethoxyfos, chlorfenvinphos, chlormephos, chlorpyrifo
  • GABA-gated chloride channel blockers such as, for example, cyclodiene-organochlorines, for example chlordane and endosulfan or phenylpyrazoles (fiproles), for example ethiprole and fipronil;
  • Sodium channel modulators such as, for example, pyrethroids, e.g. acrinathrin, allethrin, d-cis-trans allethrin, d-trans allethrin, bifenthrin, bioallethrin, bioallethrin s-cyclopentenyl isomer, bioresmethrin, cycloprothrin, cyfluthrin, beta-cyfluthrin, cyhalothrin, lambda-cyhalothrin, gamma-cyhalothrin, cypermethrin, alpha-cypermethrin, beta-cypermethrin, theta-cypermethrin, zeta-cypermethrin, cyphenothrin [(lR)-trans-isomer], deltamethrin, empenthrin [(EZ)-(lR)-i
  • Nicotinic acetylcholine receptor (nAChR) competitive modulators such as, for example, neonicotinoids, e.g. acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, thiacloprid and thiamethoxam or nicotine or sulfoxaflor or flupyradifurone;
  • neonicotinoids e.g. acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, thiacloprid and thiamethoxam or nicotine or sulfoxaflor or flupyradifurone
  • Nicotinic acetylcholine receptor (nAChR) allosteric modulators such as, for example, spinosyns, e.g. spinetoram and spinosad;
  • Glutamate-gated chloride channel (GluCl) allosteric modulators such as, for example, avermectins/milbemycins, for example abamectin, emamectin benzoate, lepimectin and milbemectin;
  • Juvenile hormone mimics such as, for example, juvenile hormone analogues, e.g. hydroprene, kinoprene and methoprene or fenoxycarb or pyriproxyfen;
  • Miscellaneous non-specific (multi-site) inhibitors such as, for example, alkyl halides, e.g. methyl bromide and other alkyl halides; or chloropicrine or sulphuryl fluoride or borax or tartar emetic or methyl isocyanate generators, e.g. diazomet and metam;
  • Mite growth inhibitors such as, for example clofentezine, hexythiazox and diflovidazin or etoxazole;
  • Microbial disruptors of the insect gut membrane such as, for example Bacillus thuringiensis subspecies israelensis, Bacillus sphaericus, Bacillus thuringiensis subspecies aizawai, Bacillus thuringiensis subspecies kurstaki, Bacillus thuringiensis subspecies tenebrionis, and B.t. plant proteins: CrylAb, Cry 1 Ac, Cry 1 Fa, Cry 1 A.105, Cry2Ab, Vip3A, mCry3A, Cry3Ab, Cry3Bb, Cry34Abl/35Abl;
  • Inhibitors of mitochondrial ATP synthase such as, ATP disruptors such as, for example, diafenthiuron or organotin compounds, for example azocyclotin, cyhexatin and fenbutatin oxide or propargite or tetradifon;
  • Nicotinic acetylcholine receptor channel blockers such as, for example, bensultap, cartap hydrochloride, thiocylam, and thiosultap-sodium;
  • Inhibitors of chitin biosynthesis, type 0, such as, for example, bistrifluron, chlorfluazuron, diflubenzuron, flucycloxuron, flufenoxuron, hexaflumuron, lufenuron, novaluron, noviflumuron, teflubenzuron and triflumuron;
  • Inhibitors of chitin biosynthesis type 1, for example buprofezin;
  • Moulting disruptor in particular for Diptera, i.e. dipterans), such as, for example, cyromazine;
  • Ecdysone receptor agonists such as, for example, chromafenozide, halofenozide, methoxyfenozide and tebufenozide;
  • Octopamine receptor agonists such as, for example, amitraz
  • Mitochondrial complex III electron transport inhibitors such as, for example, hydramethylnone or acequinocyl or fluacrypyrim;
  • Mitochondrial complex I electron transport inhibitors such as, for example from the group of the METI acaricides, e.g. fenazaquin, fenpyroximate, pyrimidifen, pyridaben, tebufenpyrad and tolfenpyrad or rotenone (Derris);
  • Voltage-dependent sodium channel blockers such as, for example indoxacarb or metaflumizone
  • Inhibitors of acetyl CoA carboxylase such as, for example, tetronic and tetramic acid derivatives, e.g. spirodiclofen, spiromesifen and spirotetramat;
  • Mitochondrial complex IV electron transport inhibitors such as, for example, phosphines, e.g. aluminium phosphide, calcium phosphide, phosphine and zinc phosphide or cyanides, e.g. calcium cyanide, potassium cyanide and sodium cyanide;
  • Mitochondrial complex II electron transport inhibitors such as, for example, beta-ketonitrile derivatives, e.g. cyenopyrafen and cyflumetofen and carboxanilides, such as, for example, pyflubumide;
  • Ryanodine receptor modulators such as, for example, diamides, e.g. chlorantraniliprole, cyantraniliprole and flubendiamide,
  • the Insecticides and combinations thereof may be used in a curative or preventive manner.
  • Insecticides especially used in the context of the present invention are selected from the group 1-2 consisting of:
  • Insecticides are selected from the group 1-3 consisting of:
  • Clothianidin Spirotetramat, Flupyradifurone, Tetraniliprole, Bacillus firmus GB126, Imidacloprid, Flubendiamide, Deltamethrin, Thiacloprid, Spiromesifen, Ethiprole, Paecilomyces lilacinus 251, Terpenoid blend (e.g.
  • Insecticides are selected from the group 1-4 consisting of:
  • Clothianidin Spirotetramat, Flupyradifurone, Tetraniliprole, Bacillus firmus GB126, Imidacloprid, Flubendiamide, Deltamethrin, Thiacloprid, Spiromesifen, Ethiprole, Paecilomyces lilacinus 251, Terpenoid blend (e.g. Requiem®) and Fipronil.
  • Insecticides are selected from the group 1-5 consisting of:
  • Insecticides are selected from the group 1-6 consisting of:
  • the fungicidal active ingredient are selected from the group F-1 consisting of fifteen subgroups being
  • Inhibitors of the ergosterol biosynthesis for example (1.001) cyproconazole, (1.002) difenoconazole, (1.003) epoxiconazole, (1.004) fenhexamid, (1.005) fenpropidin, (1.006) fenpropimorph, (1.007) fenpyrazamine, (1.008) fluquinconazole, (1.009) flutriafol, (1.010) imazalil, (1.011) imazalil sulfate, (1.012) ipconazole, (1.013) metconazole, (1.014) myclobutanil, (1.015) paclobutrazol, (1.016) prochloraz, (1.017) propiconazole, (1.018) prothioconazole, (1.019) Pyrisoxazole, (1.020) spiroxamine, (1.021) tebuconazole, (1.022) tetraconazole, (1.023) t
  • Inhibitors of the respiratory chain at complex I or II for example (2.001) benzovindiflupyr, (2.002) bixafen, (2.003) boscalid, (2.004) carboxin, (2.005) fluopyram, (2.006) flutolanil, (2.007) fluxapyroxad, (2.008) furametpyr, (2.009) Isofetamid, (2.010) isopyrazam (anti-epimeric enantiomer 1R,4S,9S), (2.011) isopyrazam (anti-epimeric enantiomer 1S,4R,9R), (2.012) isopyrazam (anti-epimeric racemate 1RS,4SR,9SR), (2.013) isopyrazam (mixture of syn-epimeric racemate 1RS,4SR,9RS and anti-epimeric racemate 1RS,4SR,9SR), (2.014) isopyrazam (syn-epimeric enantiomer 1R,4S
  • Inhibitors of the respiratory chain at complex III for example (3.001) ametoctradin, (3.002) amisulbrom, (3.003) azoxystrobin, (3.004) coumethoxystrobin, (3.005) coumoxystrobin, (3.006) cyazofamid, (3.007) dimoxystrobin, (3.008) enoxastrobin, (3.009) famoxadone, (3.010) fenamidone, (3.011) flufenoxystrobin, (3.012) fluoxastrobin, (3.013) kresoxim-methyl, (3.014) metominostrobin, (3.015) orysastrobin, (3.016) picoxystrobin, (3.017) pyraclostrobin, (3.018) pyrametostrobin, (3.019) pyraoxystrobin, (3.020) trifloxystrobin, (3.021) (2E)-2- ⁇ 2-[( ⁇ [(lE)-l-(3- ⁇ [((l
  • Inhibitors of the mitosis and cell division for example (4.001) carbendazim, (4.002) diethofencarb, (4.003) ethaboxam, (4.004) fluopicolide, (4.005) pencycuron, (4.006) thiabendazole, (4.007) thiophanate- methyl, (4.008) zoxamide, (4.009) 3-chloro-4-(2,6-difluorophenyl)-6-methyl-5-phenylpyridazine, (4.010) 3-chloro-5-(4-chlorophenyl)-4-(2,6-difluorophenyl)-6-methylpyridazine, (4.011) 3-chloro-5-(6- chloropyridin-3-yl)-6-methyl-4-(2,4,6-trifluorophenyl)pyridazine, (4.012) 4-(2-bromo-4-fluorophenyl)-N- (2,6-difluorophenyl)-
  • Compounds capable to induce a host defence for example (6.001) acibenzolar-S-methyl, (6.002) isotianil, (6.003) probenazole, (6.004) tiadinil;
  • Inhibitors of the amino acid and/or protein biosynthesis for example (7.001) cyprodinil, (7.002) kasugamycin, (7.003) kasugamycin hydrochloride hydrate, (7.004) oxytetracycline, (7.005) pyrimethanil, (7.006) 3-(5-fluoro-3,3,4,4-tetramethyl-3,4-dihydroisoquinolin-l-yl)quinolone;
  • Inhibitors of the ATP production for example (8.001) silthiofam; 9) Inhibitors of the cell wall synthesis, for example (9.001) benthiavalicarb, (9.002) dimethomorph, (9.003) flumorph, (9.004) iprovalicarb, (9.005) mandipropamid, (9.006) pyrimorph, (9.007) valifenalate, (9.008) (2E)-3-(4-tert-butylphenyl)-3-(2-chloropyridin-4-yl)-l-(morpholin-4-yl)prop-2-en-l-one, (9.009) (2Z)-3-(4-tert-butylphenyl)-3-(2-chloropyridin-4-yl)-l-(morpholin-4-yl)prop-2-en-l-one;
  • Inhibitors of the lipid and membrane synthesis for example (10.001) propamocarb, (10.002) propamocarb hydrochloride, (10.003) tolclofos-methyl;
  • Inhibitors of the melanin biosynthesis for example (11.001) tricyclazole, (11.002) 2,2,2-trifluoroethyl ⁇ 3-methyl-l-[(4-methylbenzoyl)amino]butan-2-yl ⁇ carbamate;
  • Inhibitors of the nucleic acid synthesis for example (12.001) benalaxyl, (12.002) benalaxyl-M (kiralaxyl), (12.003) metalaxyl, (12.004) metalaxyl-M (mefenoxam); 13) Inhibitors of the signal transduction, for example (13.001) fludioxonil, (13.002) iprodione, (13.003) procymidone, (13.004) proquinazid, (13.005) quinoxyfen, (13.006) vinclozolin;
  • Fungicides used in the context of the present invention are selected from the group F-2 consisting of:
  • Preferred Fungicides are selected from the group F-3 consisting of: (3S,6S,7R,8R)-8-benzyl-3-[( ⁇ 3-[(isobutyryloxy)methoxy]-4-methoxypyridin-2-yl ⁇ carbonyl)amino]-6- methyl-4,9-dioxo-l,5-dioxonan-7-yl 2-methylpropanoate, l-(4- ⁇ 4-[(5R)-5-(2,6-difluorophenyl)-4,5- dihydro- 1 ,2-oxazol-3-yl] - 1 ,3-thiazol-2-yl Jpiperidin- 1 -yl)-2- [5-methyl-3-(trifluoromethyl)- 1 H-pyrazol- 1 - yljethanone, 2,6-dimethyl-lH,5H-[l,4]dithiino[2,3-c:5,6-c']di
  • Preferred mixtures of fungicides used in the context of the present invention are selected from the group F4 consisting of : mixtures of (3S,6S,7R,8R)-8-benzyl-3-[( ⁇ 3-[(isobutyryloxy)methoxy]-4-methoxypyridin-2- yl ⁇ carbonyl)amino]-6-methyl-4,9-dioxo-l,5-dioxonan-7-yl 2-methylpropanoate and other fungicides mentioned herein, mixtures of 2,6-dimethyl-lH,5H-[l,4]dithiino[2,3-c:5,6-c']dipyrrole-l,3,5,7(2H,6H)- tetrone, esters and/or salts thereof and other fungicides mentioned herein, mixtures of 2- ⁇ 3-[2-(l- ⁇ [3,5- bis(difluoromethyl)- lH-pyrazol- 1 -y
  • Particularly preferred mixtures of fungicides used in the context of the present invention are selected from the group F-5 consisting of : mixtures of Prothioconazole and Fluopyram, mixtures of Prothioconazole and Tebuconazole, mixtures of Prothioconazole, Bixafen, and Tebuconazole,
  • Trifloxystrobin and Propineb mixtures of Trifloxystrobin and Pyrimethanil, mixtures of Trifloxystrobin and Fosetyl or salts and esters thereof, in particular Fosetyl-aluminium,
  • Beta spp. plants preferably Beta vulgaris plants, in particular sugar beet plants
  • seed treatment preferably Beta vulgaris plants, in particular sugar beet plants
  • Seeds can be coated with a coating comprising a compound selected from the following fungicides, insecticides, safeners and/or biologies.
  • Preferred fungicides for seed treatment of seeds of plants comprising the events and alleles as disclosed below in table A are selected from the group consisting of fungicides, insecticides, biologies as disclosed below.
  • Preferred fungicides for seed treatment of seeds are selected from the group SG-1 consisting of
  • Benzovindiflupyr Carbendazim, Carboxin, Difenoconazole, Ethaboxam, Fludioxonil, Fluquinconazole, Fluxapyroxad, Ipconazole, Ipfentrifluconazolejsotianil, Mefenoxam, Mefentrifluconazole, Metalaxyl, Pencycuron, Penflufen, Penthiopyrad, Prothioconazole, Prochloraz, Pyraclostrobin, Sedaxane, Silthiofam, Tebuconazole, Trifloxystrobin, Triticonazole, Ethaboxam (SCC), Penthiopyrad (DPX pipeline), Benzovindiflupyr (SYN pipeline), Bixafen, (see biologicals), Dimethomorph, Fenamidone, Fluopicolide, Fluoxastrobin, Flutolanil, Tolclophos-methyl, Azoxystrobin, Chlorothalonil,
  • Preferred insecticides for seed treatment of seeds are selected from the group SG-2 consisting of
  • Preferred safeners for seed treatment of seeds are selected from the group SG-3 consisting of
  • Biologicals and biological active ingredients for seed treatment of seeds are selected from the group SG-4 consisting of
  • Bacilus firmus especially Bacillus firmus GB126, Bacillus subtilis, Bacillus pumilus especially Bacillus pumilus QST 2808, Bacillus pumilus GB34, Pasteuria usage strains (e.g. as used in ECONEM), Rhizobium spp, especially Rhizobium tropici SP25, Pseudomonas fluorescens, Pseudomonas chloropsis, Penicilium bilaii, Rhizobium japonicum, Purnate Varroacide, Chenopodioum quinoa saponins, a recombinant exosporium-producing Bacillus thuringienses cell (especially B. thuringiensis BT013A) that expresses a fusion protein comprising:
  • More preferred Biologicals and biological active ingredients for seed treatment of seeds are selected from the group SG-5 consisting of: Bacilus firmus, especially Bacillus firmus GB126, Bacillus subtilis, Bacillus pumilus especially Bacillus pumilus QST 2808, Bacillus pumilus GB34, a recombinant exosporium-producing Bacillus thuringienses cell (especially B. thuringiensis BT013A) that expresses a fusion protein comprising:
  • a targeting sequence having at least about 43% identity with amino acids 20-35 of Sequence 1 as herein defined, wherein the identity with amino acids 25-35 is at least about 54%
  • a recombinant exosporium-producing Bacillus thuringiensis cell that expresses a fusion protein comprising: 1) a phosphoplipase having the amino acid sequence of Sequence 3 as herein defined and
  • preferred active ingredients for seed treatment of seeds are selected from the group SG-6 consisting of: ⁇ -Cyfluthrin, Bradyrhizobium japonicum, Carbendazim, Carboxin Clothianidin, Cypermethrin, Deltamethrin, Difenoconazole, Ethoxysulfuron, Fenamidon, Fenoxaprop-P-Ethyl, Flubendiamide, Fluopicolide, Fluopyram, Fluoxastrobin, Fluquinconazole, Fosetyl-Al, Fipronil, Prochloraz, Propineb, Prothioconazole, Pseudomonas Fluorescens, Spirotetramat, Tebuconazole, Tembotrione, Thiacloprid, Thiodicarb, Thiram, Triadimenol, Trifloxystrobin and Triflumuron.
  • Particularly preferred combinations of active ingredients for seed treatment used in the context of the present invention are selected from: ⁇
  • a combination of active ingredients for seed treatment is selected from the group SG-8 consisting of: combination of Clothianidin and B. firmus GB126,
  • increasing yield means a significant increase in yield by compared with the untreated plant, preferably a significant increase by at least 1% such as by 1% to 3% , compared with the untreated plant (100% yield), i.e. the yield of the treated plants is at least 101% compared to the yield (100%) of the untreated plant; more preferably, the yield is even more increased ba at least 2%, more preferably at least 5% such as by 2% to 5% (yield from 102% to 105%), by 2 to 10 % (yield from 102% to 110%) or by 5% to 20% (yield of froml05% to 120 %).
  • the yield increase may be achieved by curative treatment, i.e. for treatment of already infected plants, or by protective treatment, for protection of plants which have not yet been infected.
  • to treat with means to contact a plant or part of a plant with an effective amount of an active ingredient (AI) or a combination thereof or to coat a seed with an active AI or a combination thereof.
  • AI active ingredient
  • Active ingredient shall refer to compounds used in agriculture. Agriculture encompasses the production of food and feed crops, forestry, the protection of stored products including food, feed but also other materials of plant origin. Preferably agriculture shall encompass the production of food and feed crops, forestry, the protection of stored products being food, feed, and materials of plant origin. Active ingredients according to the invention are not applied to humans or animals as a medical or therapeutic treatment.
  • Insecticides shall refer to active ingredients which are capable of controlling phytopathogenic pests including but not limited to insects, mites, nematodes.
  • Beta vulgaris especially to sugar beet.
  • the active ingredients according to the invention or to be used according to the invention may be a composition (i. e. a physical mixture) comprising at least one active ingredient. It may also be a combination of active ingredients composed from separate formulations of a single active ingredient component being active ingredient (tank-mix). Another example of a combination of active ingredients according to the invention is that the active ingredients are not present together in the same formulation, but packaged separately (combipack), i.e., not jointly preformulated.
  • combipacks include one or more separate containers such as vials, cans, bottles, pouches, bags or canisters, each container containing a separate component for an agrochemical composition, here at least one active ingredient.
  • a two-component combipack is a two-component combipack.
  • the present invention also relates to a two-component combipack, comprising a first component which in turn comprises an active ingredient, a liquid or solid carrier and, if appropriate, at least one surfactant and/or at least one customary auxiliary, and a second component which in turn comprises another active ingredient, a liquid or solid carrier and, if appropriate, at least one surfactant and/or at least one customary auxiliary. More details, e.g. as to suitable liquid and solid carriers, surfactants and customary auxiliaries are described below.
  • a mixture or combination according to the invention shall mean/encompass a tank mix or a combipack.
  • an active ingredient or a combination of active ingredients as described above is used for increasing yield in Beta spp. plants.
  • one embodiment also refers to a method to increase yield of a plant as described herein comprising the step of treating a plant or part of a plant as described herein with an effective amount of an active ingredient or a combination thereof.
  • Beta spp. plants comprising an event or allele as disclosed in table A below can be treated with an active ingredient or a combination of active ingredients as described in groups H-l, H- 2, H-3, H-4, H-5, P-l, SAF-1, SAF-2, SAF-3, SAF-4, B-l, B-2, B-3, B-4, B-5, 1-1, 1-2, 1-3, 1-4, 1-5, 1-6, F-l, F-2, F-3, F-4, F-5, F-6, SG-1, SG-2, SG-3, SG-4, SG-5, SG-6, SG-7, SG-8.
  • Beta vulgaris plants, in particular sugar beet plants suitable for treatments according to the invention are plants comprising the events or alleles listed in Table A.
  • the events are characterized by reference to patent applications (column PA - incorporated by reference) or regulatory files (column RF -incorporated by reference) wherein they are described.
  • Transgenic plants comprising the events can also be described by reference to the SEQ ID entries of the Sequence listings of the referenced patent applications (column CS- herein incorporated by reference). These event characterizing sequences correspond to 5'flanking regions and junction sequences, 3' flanking regions and junction sequences, inserted transgene sequences, and/or complete sequences of the transgenic insert in the event.
  • Transgenic plants suitable for treatments according to the invention comprise at least one, preferably all of the reference sequences.
  • Beta spp. plants suitable for treatments according to the invention are transgenic Beta spp. plants comprising the event characterized by the following OECD number: KM- 00071-4.
  • Beta spp. plants suitable for treatments according to the invention are transgenic Beta spp. plants having at least one event as present in reference seeds being deposited as: NCIMB 41158 or NCIMB 41159.
  • At least one active ingredient selected from group H-l is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group H-2 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group H-3 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group H-4 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group H-5 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group P-l is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group SAF-1 is used on a plant comprising EVENT1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group SAF-2 is used on a plant comprising EVENT1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group SAF-3 is used on a plant comprising EVENT1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group SAF-4 is used on a plant comprising EVENT1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group B-l is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group B-2 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group B-3 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group B-4 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group B-5 is used on a plant comprising EVENT1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group I-l is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield. In one embodiment at least one active ingredient selected from group 1-2 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group 1-3 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group 1-4 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group 1-5 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group 1-6 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group F- 1 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group F-2 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group F-3 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group F-4 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group F-5 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group F-6 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group SG-1 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group SG-2 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group SG-3 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group SG-4 is used on a plant comprising EVENT1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group SG-5 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield. In one embodiment at least one active ingredient selected from group SG-6 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group SG-7 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • At least one active ingredient selected from group SG-8 is used on a plant comprising EVENT 1 or plant parts thereof, preferably for increasing yield.
  • Beta spp. plants and plant parts with active ingredients or mixtures thereof is carried out directly or by acting on the environment, habitat or storage space using customary treatment methods, for example by dipping, spraying, atomizing, misting, evaporating, dusting, fogging, scattering, foaming, painting on, spreading, injecting, drenching, trickle irrigation and, in the case of propagation material, in particular in the case of seed, furthermore by the dry seed treatment method, the wet seed treatment method, the slurry treatment method, by encrusting, by coating with one or more coats and the like. It is furthermore possible to apply the active ingredients or mixtures thereof by the ultra-low volume method or to inject the active ingredient preparation or the active ingredient itself into the soil.
  • a preferred direct treatment of the plants is the leaf application treatment, i.e. active ingredients or mixtures thereof or compositions comprising active ingredients or mixtures thereof are applied to the foliage, it being possible for the treatment frequency and the application rate to be matched to achieve the yield increase in question.
  • active ingredients or mixtures thereof or compositions comprising active ingredients or mixtures thereof reach the plants via the root system.
  • the treatment of the plants is effected by allowing active ingredients or mixtures thereof or compositions comprising active ingredients or mixtures thereof to act on the environment of the plant. This can be done for example by drenching, incorporating in the soil or into the nutrient solution, i.e. the location of the plant (for example the soil or hydroponic systems) is impregnated with a liquid form of active ingredients or mixtures thereof or compositions comprising active ingredients or mixtures thereof, or by soil application, i.e. the active ingredients or mixtures thereof or compositions comprising active ingredients or mixtures thereof are incorporated into the location of the plants in solid form (for example in the form of granules).
  • the inventive use exhibits the advantages described on Beta spp. plants, plant parts thereof, plant propagation material or the soil in which Beta spp. plants are grown or intended to be grown in spray application, in seed treatment, in drip and drench applications, in-furrow application, chemigation, i.e. by addition of active ingredients or mixtures thereof to the irrigation water, and in hydroponic/mineral systems.
  • the combined use of active ingredients or mixtures thereof or compositions comprising active ingredients or mixtures thereof, with genetically modified cultivars, especially of transgenic Beta spp. cultivars, is additionally likewise possible.
  • the application rates can be varied within a relatively wide range, depending on the kind of application.
  • the application rate of the inventive active ingredients is generally known to the skilled person. Generally, the following ranges can be applied
  • leaves from 0.1 to 10 000 g/ha, preferably from 10 to 1000 g/ha, more preferably from 50 to 500 g/ha (in the case of application by watering or dripping, it is even possible to reduce the application rate, especially when inert substrates such as rockwool or perlite are used);
  • ⁇ in the case of soil treatment from 0.1 to 10 000 g/ha, preferably from 1 to 5000 g/ha.
  • the present invention further relates to a composition for increasing yield, comprising at least one active ingredient or mixtures thereof.
  • active ingredient or mixtures thereof.
  • These are preferably fungicidal, bactericidal, herbicidal, insecticidal, acaricidal, or nematicidal compositions which comprise agriculturally suitable auxiliaries, solvents, carriers, surfactants or extenders.
  • a carrier is a natural or synthetic, organic or inorganic substance with which the active ingredients are mixed or combined for better applicability, in particular for application to plants or plant parts or seed.
  • the carrier which may be solid or liquid, is generally inert and should be suitable for use in agriculture.
  • Useful solid carriers include: for example ammonium salts and natural rock flours, such as kaolins, clays, talc, chalk, quartz, attapulgite, montmorillonite or diatomaceous earth, and synthetic rock flours, such as finely divided silica, alumina and silicates; useful solid carriers for granules include: for example, crushed and fractionated natural rocks such as calcite, marble, pumice, sepiolite and dolomite, and also synthetic granules of inorganic and organic flours, and granules of organic material such as paper, sawdust, coconut shells, maize cobs and tobacco stalks; useful emulsifiers and/or foam-formers include: for example nonionic and anionic emulsifiers, such as polyoxyethylene fatty acid esters, polyoxyethylene fatty alcohol ethers, for example alkylaryl polyglycol ethers, alkylsulphonates, alkyl sulphates, aryl
  • oligo- or polymers for example those derived from vinylic monomers, from acrylic acid, from EO and/or PO alone or in combination with, for example, (poly)alcohols or (poly)amines. It is also possible to use lignin and its sulphonic acid derivatives, unmodified and modified celluloses, aromatic and/or aliphatic sulphonic acids and also their adducts with formaldehyde.
  • the active ingredients or mixtures thereof can be converted to the customary formulations, such as solutions, emulsions, wettable powders, water- and oil-based suspensions, powders, dusts, pastes, soluble powders, soluble granules, granules for broadcasting, suspoemulsion concentrates, natural products impregnated with active ingredient, synthetic substances impregnated with active ingredients or mixtures thereof, fertilizers and also microencapsulations in polymeric substances.
  • customary formulations such as solutions, emulsions, wettable powders, water- and oil-based suspensions, powders, dusts, pastes, soluble powders, soluble granules, granules for broadcasting, suspoemulsion concentrates, natural products impregnated with active ingredient, synthetic substances impregnated with active ingredients or mixtures thereof, fertilizers and also microencapsulations in polymeric substances.
  • the active ingredients or mixtures thereof can be applied as such, in the form of their formulations or the use forms prepared therefrom, such as ready-to-use solutions, emulsions, water- or oil-based suspensions, powders, wettable powders, pastes, soluble powders, dusts, soluble granules, granules for broadcasting, suspoemulsion concentrates, natural products impregnated with active ingredient, synthetic substances impregnated with active ingredient, fertilizers and also microencapsulations in polymeric substances.
  • Application is accomplished in a customary manner, for example by watering, spraying, atomizing, broadcasting, dusting, foaming, spreading-on and the like. It is also possible to deploy the active ingredients by the ultra-low volume method or to inject the active ingredient preparation/the active ingredient itself into the soil. It is also possible to treat the seed of the plants.
  • the formulations mentioned can be prepared in a manner known per se, for example by mixing the active ingredients or mixtures thereof with at least one customary extender, solvent or diluent, emulsifier, dispersant and/or binder or fixing agent, wetting agent, a water repellent, if appropriate siccatives and UV stabilizers and if appropriate dyes and pigments, antifoams, preservatives, secondary thickeners, stickers, gibberellins and also other processing auxiliaries.
  • the present invention includes not only formulations which are already ready for use and can be deployed with a suitable apparatus to the plant or the seed, but also commercial concentrates which have to be diluted with water prior to use.
  • active ingredients or mixtures thereof may be present as such or in their (commercial) formulations and in the use forms prepared from these formulations as a mixture with other (known) active ingredients, such as insecticides, attractants, sterilants, bactericides, acaricides, nematicides, fungicides, growth regulators, herbicides, fertilizers, safeners and/or semiochemicals.
  • active ingredients such as insecticides, attractants, sterilants, bactericides, acaricides, nematicides, fungicides, growth regulators, herbicides, fertilizers, safeners and/or semiochemicals.
  • auxiliaries used may be those substances which are suitable for imparting particular properties to the composition itself or and/or to preparations derived therefrom (for example spray liquors, seed dressings), such as certain technical properties and/or also particular biological properties.
  • Typical auxiliaries include: extenders, solvents and carriers.
  • Suitable extenders are, for example, water, polar and nonpolar organic chemical liquids, for example from the classes of the aromatic and nonaromatic hydrocarbons (such as paraffins, alkylbenzenes, alkylnaphthalenes, chlorobenzenes), the alcohols and polyols (which may optionally also be substituted, etherified and/or esterified), the ketones (such as acetone, cyclohexanone), esters (including fats and oils) and (poly)ethers, the unsubstituted and substituted amines, amides, lactams (such as N-alkylpyrrolidones) and lactones, the sulphones and sulphoxides (such as dimethyl sulphoxide).
  • aromatic and nonaromatic hydrocarbons such as paraffins, alkylbenzenes, alkylnaphthalenes, chlorobenzenes
  • the alcohols and polyols which may optionally also
  • Liquefied gaseous extenders or carriers are understood to mean liquids which are gaseous at standard temperature and under standard pressure, for example aerosol propellants such as halohydrocarbons, or else butane, propane, nitrogen and carbon dioxide.
  • tackifiers such as carboxymethylcellulose, natural and synthetic polymers in the form of powders, granules or latices, such as gum arabic, polyvinyl alcohol and polyvinyl acetate, or else natural phospholipids such as cephalins and lecithins and synthetic phospholipids.
  • Further additives may be mineral and vegetable oils.
  • Useful liquid solvents are essentially: aromatics such as xylene, toluene or alkylnaphthalenes, chlorinated aromatics or chlorinated aliphatic hydrocarbons such as chlorobenzenes, chloroethylenes or methylene chloride, aliphatic hydrocarbons such as cyclohexane or paraffins, for example petroleum fractions, alcohols such as butanol or glycol and their ethers and esters, ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone, strongly polar solvents such as dimethylformamide and dimethyl sulphoxide, or else water.
  • aromatics such as xylene, toluene or alkylnaphthalenes
  • chlorinated aromatics or chlorinated aliphatic hydrocarbons such as chlorobenzenes, chloroethylenes or methylene chloride
  • aliphatic hydrocarbons such as
  • compositions comprising active ingredients or mixtures thereof may additionally comprise further components, for example surfactants.
  • surfactants are emulsifiers and/or foam formers, dispersants or wetting agents having ionic or nonionic properties, or mixtures of these surfactants.
  • Examples thereof are salts of polyacrylic acid, salts of lignosulphonic acid, salts of phenolsulphonic acid or naphthalenesulphonic acid, polycondensates of ethylene oxide with fatty alcohols or with fatty acids or with fatty amines, substituted phenols (preferably alkylphenols or arylphenols), salts of sulphosuccinic esters, taurine derivatives (preferably alkyl taurates), phosphoric esters of polyethoxylated alcohols or phenols, fatty esters of polyols, and derivatives of the compounds containing sulphates, sulphonates and phosphates, for example alkylaryl polyglycol ethers, alkylsulphonates, alkyl sulphates, arylsulphonates, protein hydrolysates, lignosulphite waste liquors and methylcellulose.
  • a surfactant is necessary if one of the active ingredients and/or one of the inert carriers is insoluble in water and when application is effected in water.
  • the proportion of surfactants is between 5 and 40 per cent by weight of the inventive composition.
  • dyes such as inorganic pigments, for example iron oxide, titanium oxide and Prussian Blue, and organic dyes such as alizarin dyes, azo dyes and metal phthalocyanine dyes, and trace nutrients such as salts of iron, manganese, boron, copper, cobalt, molybdenum and zinc.
  • Further additives may be perfumes, mineral or vegetable, optionally modified oils, waxes and nutrients (including trace nutrients), such as salts of iron, manganese, boron, copper, cobalt, molybdenum and zinc.
  • Additional components may be stabilizers, such as cold stabilizers, preservatives, antioxidants, light stabilizers, or other agents which improve chemical and/or physical stability.
  • additional components may also be present, for example protective colloids, binders, adhesives, thickeners, thixotropic substances, penetrants, stabilizers, sequestering agents, complex formers.
  • the active ingredients or mixtures thereof can be combined with any solid or liquid additive commonly used for formulation purposes.
  • the formulations contain generally between 0.05 and 99% by weight, 0.01 and 98% by weight, preferably between 0.1 and 95% by weight, more preferably between 0.5 and 90% of active ingredients or mixtures thereof, most preferably between 10 and 70 per cent by weight.
  • compositions comprising actives ingredients or mixture thereof are applied to the Beta spp. plants and/or in their habitat.
  • Beta spp. plants, and plant parts can be treated.
  • plants is meant all plants and plant populations such as desirable and undesirable wild plants, cultivars and plant varieties (whether or not protectable by plant variety or plant breeder's rights).
  • Cultivars and plant varieties can be plants obtained by conventional propagation and breeding methods which can be assisted or supplemented by one or more biotechnological methods such as by use of double haploids, protoplast fusion, random and directed mutagenesis, molecular or genetic markers or by bioengineering and genetic engineering methods.
  • plant parts are meant all above ground and below ground parts and organs of plants such as shoot, leaf, blossom, bud, bolls and root, whereby for example leaves, needles, stems, branches, blossoms, buds, tiller, fruiting bodies, bolls, fruits and seed as well as roots, corms and rhizomes are listed.
  • Crops and vegetative and generative propagating material for example cuttings, corms, rhizomes, runners, slips and seeds also belong to plant parts. Seeds are preferred plant parts.
  • Beta spp. plants, plant parts or seeds are hybrid plants, plant parts including seeds.
  • Beta spp. plants, plant parts including seeds are tolerant to one or more of the herbicides selected from the group of glufosinate, glyphosate (tradename RoundupReady), imazamethabenz, imazamethabenz-methyl, imazamox, imazamox-ammonium, imazapic, imazapic- ammonium, imazapyr, imazapyr-isopropyl-ammonium, imazaquin, imazaquin- ammonium, imazethapyr, imazethapyr- ammonium, atrazine, simazine.
  • the Beta spp. plants, plant parts including seeds are insect resistant to one or more of or more Beta spp. plant pests.
  • growth stage refers to the growth stages as defined by the BBCH Codes in "Growth stages of mono- and dicotyledonous plants", 2nd edition 2001, edited by Uwe Meier from the Federal Biological Research Centre for Agriculture and Forestry.
  • the BBCH codes are a well-established system for a uniform coding of phonologically similar growth stages of all mono- and dicotyledonous plant species.
  • the abbreviation BBCH derives from "Bisammlungtician, Bundessortenamt und Chemische Industrie”.
  • Plant cultivars are understood to mean plants which have new properties ("traits") and which have been obtained by conventional breeding, by mutagenesis or with the aid of recombinant DNA techniques.
  • Crop plants may accordingly be plants which can be obtained by conventional breeding and optimization methods or by biotechnology and genetic engineering methods or combinations of these methods, including the transgenic plants and including the plant varieties which can and cannot be protected by plant variety rights.
  • GMOs genetically modified organisms
  • Genetically modified plants are plants in which a heterologous gene has been integrated stably into the genome.
  • heterologous gene means essentially a gene which is provided or assembled outside the plant and which, on introduction into the cell nucleus genome, imparts new or improved agronomic or other properties to the chloroplast genome or the mitochondrial genome of the transformed plant by virtue of it expressing a protein or polypeptide of interest or by virtue of another gene which is present in the plant, or other genes which are present in the plant, being downregulated or silenced (for example by means of antisense technology, co-suppression technology or RNAi technology [RNA interference]).
  • a heterologous gene present in the genome is likewise referred to as a transgene.
  • a transgene which is defined by its specific presence in the plant genome is referred to as a transformation or transgenic event.
  • Plants and plant cultivars which are preferably treated according to the invention include all plants which have genetic material which imparts particularly advantageous, useful traits to these plants (whether obtained by breeding and/or biotechnological means).
  • the treatment of the seed of plants has been known for a long time and is the subject of constant improvements. Nevertheless, the treatment of seed gives rise to a series of problems which cannot always be solved in a satisfactory manner. For instance, it is desirable to develop methods for protecting the seed, the germinating plant and the resulting plants or plant parts, which dispense with, or at least significantly reduce, the additional deployment of crop protection products after planting or after emergence of the plants. It is additionally desirable to optimize the amount of active ingredients or mixtures thereof used in such a way as to provide the best possible protection for the seed and the germinating plant in order to achieve the yield increase, but without damaging the Beta spp. plant itself by the active ingredient used.
  • the present invention therefore relates more particularly also to a method for treating seed to increase yield in Beta spp. plants which grow from the seed or seedlings, by treating the Beta spp. seed with active ingredients or mixtures thereof.
  • Beta spp. plants at BBCH stage 10 or later which grow from the seed or seedlings by treating the Beta spp. seed at BBCH stage 00 with active ingredients or mixtures thereof.
  • a method for treating seed to increase yield in herbicide tolerant Beta spp. hybrid plants at BBCH stage 10 or later which grow from the seed or seedlings by treating the herbicide tolerant Beta spp. hybrid seed at BBCH stage 00 with active ingredients or mixtures thereof.
  • a method for treating seed to increase yield in insect resistant Beta spp. hybrid plants at BBCH stage 10 or later which grow from the seed or seedlings by treating the herbicide tolerant Beta spp. hybrid seed at BBCH stage 00 with active ingredients or mixtures thereof.
  • the invention likewise relates to the use of active ingredients or mixtures for treatment of seed to increase yield in the the plants or those plant parts which grow from the seeds.
  • active ingredients or mixtures thereof can especially also be used in transgenic seed.
  • the active ingredients or mixtures thereof are applied to the seed alone or in a suitable formulation.
  • the seed is treated in a state in which it is stable enough to avoid damage during treatment.
  • the seed may be treated at any time between harvest and sowing.
  • the seed typically used has been separated from the plant and freed from cobs, shells, stalks, coats, hairs or the fruit flesh.
  • seed which has been harvested, cleaned and dried to a moisture content of less than 15% by weight.
  • the active ingredients or mixtures thereof can be applied directly, i.e. without containing any further components and without having been diluted. In general, it is preferable to apply active ingredients or mixtures thereof, to the seed in the form of a suitable formulation.
  • suitable formulations and methods for seed treatment are known to those skilled in the art and are described, for example, in the following documents: US 4,272,417 A, US 4,245,432 A, US 4,808,430 A, US 5,876,739 A, US 2003/0176428 Al, WO 2002/080675 Al, WO 2002/028186 A2.
  • the active ingredients or mixtures thereof can be converted to the customary seed dressing formulations, such as solutions, emulsions, suspensions, powders, foams, slurries or other coating materials for seed, and also ULV formulations.
  • formulations are produced in a known manner, by mixing the active ingredients or active ingredient combinations with customary additives, for example customary extenders and solvents or diluents, dyes, wetting agents, dispersants, emulsifiers, defoamers, preservatives, secondary thickeners, stickers, gibberellins and also water.
  • customary additives for example customary extenders and solvents or diluents, dyes, wetting agents, dispersants, emulsifiers, defoamers, preservatives, secondary thickeners, stickers, gibberellins and also water.
  • Useful dyes which may be present in the seed dressing formulations usable in accordance with the invention are all dyes customary for such purposes. It is possible to use both sparingly water-soluble pigments and water-soluble dyes. Examples include the dyes known under the Rhodamine B, C.I. Pigment Red 112 and C.I. Solvent Red 1.
  • the wetting agents which may be present in the seed dressing formulations usable in accordance with the invention include all substances which promote wetting and are customary for formulation of active agrochemical ingredients.
  • Usable with preference are alkyl naphthalenesulphonates, such as diisopropyl or diisobutyl naphthalenesulphonate.
  • the dispersants and/or emulsifiers which may be present in the seed dressing formulations usable in accordance with the invention include all nonionic, anionic and cationic disperants which are customary for formulation of active agrochemical ingredients. Usable with preference are nonionic or anionic dispersants or mixtures of nonionic or anionic dispersants. Suitable nonionic dispersants include especially ethylene oxide-propylene oxide block polymers, alkylphenol polyglycol ethers and tristyrylphenol polyglycol ethers, and the phosphated or sulphated derivatives thereof.
  • Suitable anionic dispersants are especially lignosulphonates, polyacrylic acid salts and arylsulphonate-formaldehyde condensates.
  • the defoamers which may be present in the seed dressing formulations usable in accordance with the invention include all foam-inhibiting substances customary for formulation of active agrochemical ingredients. Usable with preference are silicone defoamers and magnesium stearate.
  • the preservatives which may be present in the seed dressing formulations usable in accordance with the invention include all substances usable for such purposes in agrochemical formulations. Examples include dichlorophene and benzyl alcohol hemiformal.
  • Useful secondary thickeners which may be present in the seed dressing formulations usable in accordance with the invention include all substances usable for such purposes in agrochemical formulations.
  • Preferred examples include cellulose derivatives, acrylic acid derivatives, xanthan, modified clays and finely divided silica.
  • Useful stickers which may be present in the seed dressing formulations usable in accordance with the invention are all customary binders usable in seed dressing compositions.
  • Preferred examples include polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol and tylose.
  • the gibberellins are known (cf. R. Wegler "Chemie der convincedstoff- und Schadlingsbekampfungsstoff" [Chemistry of Crop Protection and Pest Control Compositions], vol. 2, Springer Verlag, 1970, p. 401-412).
  • the seed dressing formulations usable in accordance with the invention can be used to treat either directly or after preceding dilution with water.
  • the seed dressing preparations usable in accordance with the invention or the dilute preparations thereof can also be used to dress seed of transgenic plants. In this case, it is also possible for additional synergistic effects to occur in interaction with substances formed by expression.
  • the seed dressing procedure is to introduce the seed into a mixer, to add the particular desired amount of seed dressing formulations, either as such or after preceding dilution with water, and to mix until the formulation is distributed homogeneously on the seed. This may be followed by a drying operation.
  • the application rate of seed dressing formulations usable in accordance with the invention may vary within a relatively wide range. It is guided by the particular content of the active ingredients in the formulations and by the seed.
  • the application rates of seed treatment compositions comprising active ingredients or mixtures thereof are: from 0.1 to 200 g per 100 kg of seed, preferably from 1 to 150 g per 100 kg of seed, more preferably from 2.5 to 25 g per 100 kg of seed, even more preferably from 2.5 to 12.5 g per 100 kg of seed.
  • the active ingredients or mixtures thereof may be present in their commercially available formulations and in the use forms, prepared from these formulations, as a mixture with other active ingredients, such as insecticides, attractants, sterilants, bactericides, acaricides, nematicides, fungicides, growth regulators, herbicides, safeners, fertilizers or semiochemicals.
  • active ingredients such as insecticides, attractants, sterilants, bactericides, acaricides, nematicides, fungicides, growth regulators, herbicides, safeners, fertilizers or semiochemicals.
  • the active ingredients may be present in their commercially available formulations and in the use forms, prepared from these formulations, as a mixture with one or more active ingredients.
  • HD-Zip homeodomain- leucine zipper
  • PAT herbicidal activity of glufosinate bar hygroscopicus (phosphinothricin) herbicides by acetylation barnase ribonuclease enzyme from causes male sterility by interfering with RNA barnase Bacillus amyloliquefaciens production in the tapetum cells of the anther barnase ribonuclease inhibitor from restores fertility by repressing the inhibitory barstar Bacillus amyloliquefaciens effect of barnase on tapetum cells of the anther protein from Arabidopsis thaliana that
  • CCOMT ccomt CoA 3-O-methyltransferase
  • RNAi guaiacyl
  • CordapA Corynebacterium glutamicum increases the production of amino acid lysine herbicide tolerant form of 5- enolpyruvulshikimate-3-phosphate decreases binding affinity for glyphosate, synthase (EPSPS) enzyme from thereby conferring increased tolerance to cp4 epsps Agrobacterium tumefaciens strain CP4 glyphosate herbicide Cry 1 A.105 protein which comprises the
  • Bacillus thuringiensis subsp. confers resistance to lepidopteran insects by crylA.105 Kumamotoensis selectively damaging their midgut lining
  • CrylAb delta-endotoxin from Bacillus confers resistance to lepidopteran insects by crylAb thuringiensis subsp. Kurstaki selectively damaging their midgut lining
  • CrylAc delta-endotoxin from Bacillus confers resistance to lepidopteran insects by cry 1 Ac thuringiensis subsp. Kurstaki strain HD73 selectively damaging their midgut lining
  • CrylF delta-endotoxin from Bacillus confers resistance to lepidopteran insects by
  • crylF gene derived from confers resistance to lepidopteran insects by crylFa2 Bacillus thuringiensis var. Aizawai selectively damaging their midgut lining
  • Cry2Ab delta-endotoxin from Bacillus confers resistance to lepidopteran insects by cry2Ab2 thuringiensis subsp. Kumamotoensis selectively damaging their midgut lining
  • Cry2Ae delta-endotoxin from Bacillus confers resistance to lepidopteran insects by cry2Ae thuringiensis subsp Dakota selectively damaging their midgut lining
  • cry3A delta endotoxin from Bacillus confers resistance to coleopteran insects by cry 3 A thuringiensis subsp. Tenebrionis selectively damaging their midgut lining
  • RNA stability cspB cold shock protein B from Bacillus subtilis and translation
  • Dgat2A from Mortierella ramanniana modifies oil profile
  • Rootworm Snf7 gene to Western Corn Rootworm mortality.
  • EPSPS modified 5-enolpyruvylshikimate-3- epsps grg23 phosphate synthase
  • ace 5 EPSPS ACE5 protein from Arthrobacter confers tolerance to glyphosate herbicides globiformis reduces desaturation of 18: 1 oleic acid to 18:2 fad2-lA no functional enzyme is produced linoleic acid; increases the levels of
  • modified p-hydroxyphenylpyruvate confers tolerance to HPPD-inhibiting herbicides hppdPF dioxygenase (hppd) enzyme from (such as isoxaflutole) by reducing the specificity W336 Pseudomonas fluorescens strain A32 for the herbicide's bioactive constituent
  • Nc.Fad3 Neurospora crassa SDA
  • an omega-3 fatty acid SDA
  • PL V orf 1 roll virus (PLRV) (PLRV) through gene silencing mechanism putative helicase domain of the potato leaf confers resistance to potato leaf roll virus
  • PLPvV orf2 roll virus (PLRV) (PLRV) through gene silencing mechanism confers resistance to potato virus Y (PVY)
  • PVY coat through "pathogen-derived resistance” protein coat protein of the potato virus Y (PVY mechanism 12:0
  • ACP thioesterase enzyme from increases the level of triacylglycerides te Umbellularia californica (bay leaf) containing esterified lauric acid (12:0)
  • beta-D-glucuronidase (GUS) enzyme from produces blue stain on treated transformed uidA Escherichia coli tissue, which allows visual selection

Abstract

L'invention concerne un procédé pour augmenter le rendement de plantesBêta spp. et de parties de plantes associées qui se développent à partir de la graine, par traitement de plantes Bêta spp et de plantes et parties de plantes avec des ingrédients actifs ou des mélanges de ceux-ci.
PCT/EP2017/060425 2016-05-17 2017-05-02 Procédé pour accroître le rendement de plantes bêta spp. WO2017198455A2 (fr)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109439570A (zh) * 2018-10-29 2019-03-08 四川大宇中和农业科技发展有限公司 一株解磷假单胞菌及其应用

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