WO2017134685A2 - Novel hydrazino compounds as btk inhibitors - Google Patents
Novel hydrazino compounds as btk inhibitors Download PDFInfo
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- WO2017134685A2 WO2017134685A2 PCT/IN2017/050048 IN2017050048W WO2017134685A2 WO 2017134685 A2 WO2017134685 A2 WO 2017134685A2 IN 2017050048 W IN2017050048 W IN 2017050048W WO 2017134685 A2 WO2017134685 A2 WO 2017134685A2
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- 0 C*C(*(C)C*(C)CC1)=C1N=C=N Chemical compound C*C(*(C)C*(C)CC1)=C1N=C=N 0.000 description 8
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
- C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
- C07D487/04—Ortho-condensed systems
Definitions
- the present invention relates to novel hydrazino compounds as Bruton tyrosine kinase (BTK) inhibitors, process of preparation thereof, and to the use of the compounds in the preparation of pharmaceutical compositions for the therapeutic treatment of disorders involving mediation of Bruton tyrosine kinase in humans.
- BTK Bruton tyrosine kinase
- BTK BTK signal transduction pathways regulating B-cell proliferation, differentiation and survival has been a ground breaking discovery and has led to developments of drugs for the treatment of B-cell malignancies.
- Improper /auto activated BTK signaling is considered to be the major cause in several haematological malignancies such as chronic lymphocytic leukemia (CLL), mantle cell lymphoma (MCL) etc.
- CLL chronic lymphocytic leukemia
- MCL mantle cell lymphoma
- Ibrutinib which is disclosed in US patent number 7514444 is presently marketed for the treatment of mantle cell lymphoma and chronic lymphocytic leukemia under the trade name Imbruvica .
- the clinical and commercial success of Ibrutinib has led to discovery of newer BTK inhibitors.
- the present invention provides a compound of Formula I and pharmaceutically acceptable salts thereof
- Rj is selected from a group consisting of hydrogen, -C 1 -4 alkyl, -C3 alkenyl, -C3 alkynyl, -C 1 -4 haloalkyl, -C2-4 haloalkenyl, -C3 haloalkylalkynyl, -C 1 -4 cyanoalkyl, -C2-4 cyanoalkenyl, -C2 cyanoalkynyl, -C2-5 O-alkyl, -C2-5 O-haloalkyl, -Cj,.(, cycloalkyl, -C 4 _6 heterocycloalkyl containing 1-3 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C 1 -4 alkyl or -CO-Q_ 4 alkyl group; and when Rj is alkenyl or alkynyl, the double and triple bond, respectively, is not on
- R2 is selected from hydrogen, -Ci_ 4 alkyl, -C3 alkenyl, -C3_ 4 alkynyl, -C 1 -4 haloalkyl, -C2 haloalkenyl, -C3_ 4 haloalkylalkynyl, -C 1 -4 cyanoalkyl, -C2-4 cyanoalkenyl, -C2 cyanoalkynyl, - C2-5 O-alkyl, -C 2 - 5 O-haloalkyl, -C 3 - 6 cycloalkyl, -CN, -C(0)R 5 , -C(S)R 5 , -S0 2 R 5 , -SOR5, - CONHSO2R5, -CSNHSO2R5, -CONHCOR5, -CSNHCOR5, -CON(d_ 4 alkyl)COR 5 , , - C(S)N(Ci-4 al
- Rs and R are independently selected from hydrogen, Ci-6 alkyl, C2-6 alkenyl, C3-7 cycloalkyl or Rg and R together forms 4 to 10 membered mono or bicyclic carbocyclic ring optionally substituted with one or more Ci_3 alkyl group or a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C 1 -4 alkyl or -CO-C 1 -4 alkyl group;
- EWG is an electron withdrawing group selected from -COOR 1 0, -CON(Rio) 2 , -CN, -NO2, - SO3H, -S0 2 N(Rio) 2 , -SO2R 1 0 wherein Rjo is selected from hydrogen, -Ci_3 alkyl and -C 4 _6 cycloalkyl;
- T is a heteroatom selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -Ci_ 4 alkyl or -CO-Ci_ 4 alkyl group; p is an integer selected from 1 and 2;
- EWG2 is an electron withdrawing group selected from -COOR 1 5, -CON(Rj5)2, -CN, -NO2, - SO 3 H, -S02N(Rj5)2, -SO2R 1 5 wherein RJS at each occurrence is independently selected from hydrogen, -Ci_3 alkyl and -C 4 _6 cycloalkyl; q is an integer selected from 1 and 2;
- R3 and R 4 are independently selected from hydrogen, halogen, Ci_6 alkyl and C3-6 cycloalkyl; ring P is 4 to 13 membered saturated or unsaturated monocyclic, bicyclic or tricyclic ring optionally containing one additional heteroatom selected from oxygen, nitrogen and sulfur; ring A is a heterocycle selected from moieties provided in Figure 3
- Q is selected from CH or nitrogen
- Rj6 and R 17 together forms a phenyl ring optionally substituted with halogen, -0-Ci_3 alkyl, - COOH, -COOd_ 3 alkyl, -C 1-3 alkyl, -OH; or Rj6 and R 17 together forms a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen and sulfur; V independently selected from R 18 , halogen, -C 1 -4 haloalkyl, -C2-4 alkenyl, -C2 alkynyl, - SR18, -OR18, -SORig, -SO2R18, -N(R 18 ) 2 , -NHCHO, -NHCOCH3, -C 4 - 6 hetrocycloalkyl, -C 4 _ 6 cycloalkenyl, -CO-Ris, -CN and -CHO wherein Ris at each occurrence is independently selected from hydrogen,
- R 19 is selected from hydrogen, halogen, hydroxyl, -N(Ci_6 alkyl)2, -NH(Ci_6 alkyl), - Cj_6 haloalkyl, -C 2- 6 alkenyl, -C 2-6 alkynyl, -Od-6 alkyl, -O d-6 alkenyl, -O d-6 alkynyl,-S- Ci-6 alkyl, -CN, -CO-C1-4 alkyl, -CO— C3-6 cycloalkyl, -COOH, -CONH 2 , -CON(d-6 alkyl) 2 , - CONH(d_6 alkyl), -NHC(O) -d-4 alkyl, -N(C 1 alkyl)C(O) d-4 alkyl and when R 19 is -OC 3 - 6 alkenyl or -O d-6 alkynyl the double and triple bond, respectively,
- Yi, Y 2 , Y3 and Y 4 are independently and appropriately selected from CH, NR2 0 , O, or S; wherein, R20 is selected from hydrogen, -C 1 -3 alkyl and -d-6 cycloalkyl;
- r is an integer selected from 1 to 3;
- D is a ring containing 5 to 13 membered aryl, fused aryl, heteroaryl, fused heteroaryl, saturated or unsaturated monocyclic, bicyclic or tricyclic carbocyclic ring containing 0-3 heteroatoms selected from nitrogen, oxygen and sulfur; optionally substituted with d_3 alkyl; or D is selected from -d-6 alkyl, -C3-6 cycloalkyl, -C1-4 alkyl-O-d ⁇ alkyl, -C3-6 cyclolkyl-O- d- 4 alkyl, -C1-4 alkyl-0-C3_6 cycloalkyl, -d_ 4 haloalkyl-O-d ⁇ alkyl, -C1-4 haloalkyl-S-d-4 alkyl and -C 1 -4 alkyl-S-C2- 4 alkyl; with the proviso that when:
- ring B and D are phenyl
- Z is selected from -0-, -S-, -NH-, -N(d_4 alkyl)- or -C(O)-;
- Ring A is selected from
- R 2 is not selected from -C(0)R 5 , -C(S)R 5 , -S0 2 R 5 , -SOR 5 wherein R 5 is R y ;
- the compounds of the present invention were found to be potent and selective BTK inhibitors and can be useful in treatment of the diseases mediated by BTK receptors.
- Salts include those formed with either organic and inorganic acids or bases.
- Pharmaceutically acceptable acid addition salts include those formed from hydrochloric, hydrobromic, sulfuric, citric, tartaric, phosphoric, acetic, trifluoroacetic, triphenylacetic, phenylacetic, succinic, oxalic, fumaric, maleic, glutamic, aspartic, oxalacetic, methanesulphonic, ethanesulphonic, p-toluenesulphonic, benzenesulphonic, naphthalenesulphonic or naphthalenedisulphonic, salicylic, glutaric, gluconic, mandelic, cinnamic, ascorbic, oleic, naphthoic, hydroxynaphthoic (for example 1- or 3-hydroxy-2- naphthoic), benzoic, 4-methoxybenzoic, 2- or 4-hydroxybenzoic, 4-ch
- Pharmaceutically acceptable base salts include ammonium salts, alkali metal salts such as those of sodium and potassium, alkaline earth metal salts such as those of calcium and magnesium and salts with organic bases such as dicyclohexylamine and N-methyl-D-glucamine.
- alkyl refers to a saturated hydrocarbon chain radical that includes solely carbon and hydrogen atoms in the backbone, either linear or branched and which is attached to the rest of the molecule by a single bond, e.g., methyl, ethyl, w-propyl, 1-methylethyl (isopropyl), «-butyl, «-pentyl, and 1,1-dimethylethyl (i-butyl).
- the alkyl chain may have 1 to 13 carbon atoms unless specified otherwise.
- alkyl groups described or claimed herein may be unsubstituted or substituted with groups selected from halogen, -Od-6 alkyl, -OC 3 - 6 cycloalkyl, CN, -COOH, -COO-d-3 alkyl, -CON(-d-3 alkyl), - N0 2 , -S0 2 d_3 alkyl, -S0 2 N-d_ 3 alkyl, -CO-d_ 3 alkyl.
- the numerical in phrases like "C1-4 alkyl" refers that there are 1 to 4 carbon atoms in the alky chain.
- alkenyl refers to a hydrocarbon chain containing at least one carbon-carbon double bond, and may have (E) or (Z) configuration.
- An alkenyl group may contain 2 to 8 carbon atoms unless specified otherwise.
- Non-limiting examples of alkenyl groups include 2- propenyl (allyl), 2-methyl-2-propenyl, and (Z)-2-butenyl.
- alkenyl groups described or claimed herein may be straight chain or branched, unsubstituted or substituted with groups selected from halogen, -OCi-6 alkyl, -OC 3 -6 cycloalkyl, CN, -COOH, -COO-d_ 3 alkyl, -CON(-d_ 3 alkyl), -N0 2 , -S0 2 d_ 3 alkyl, -S0 2 N- d_ 3 alkyl, -CO-d_ 3 alkyl.
- alkynyl refers to a hydrocarbon chain having at least one carbon-carbon triple bond.
- An alkynyl group may contain 2 to 8 carbon atoms unless specified otherwise.
- Non- limiting examples of alkynyl groups include 2-propynyl and 3-butynyl. Unless set forth or recited to the contrary all alkynyl groups described or claimed herein may form part of a straight or branched, substituted or unsubstituted chains.
- the alkynyl group may be unsubstituted or substituted with the groups selected from halogen, -Od_6 alkyl, -Od_6 cycloalkyl, CN, -COOH, -COO-d_ 3 alkyl, -CON(-d_ 3 alkyl), -N0 2 , -S0 2 d_ 3 alkyl, -S0 2 N- Ci-3 alkyl, -CO-d_ 3 alkyl.
- halo as used herein includes chloro, fluoro, bromo and iodo.
- haloalkyl haloalkeny and haloalkyny refers to halo substituted alkyl, alkenyl and alkynyl chain, respectively.
- cycloalkyl denotes a non-aromatic mono-, or multicyclic ring system of 3 to about 13 carbon atoms.
- Monocyclic rings include, but are not limited to cylcopropyl, cyclobutyl, cyclopentyl and cyclohexyl.
- Examples of simple multicyclic cycloalkyl groups include perhydronapththyl, perhydroindenyl etc; bridged multicyclic groups include adamantyl and norbornyl etc, and spriromulticyclic groups for e.g., spiro(4,4)non-2-yl.
- heterocycloalkyl refers to a cycloalkyl ring containing a heteroatom. Unless specifically specified, the heteroatom is selected from N, O and S.
- cycloalkenyl refers to a monocyclic or bicyclic, 3 to 14 membered ring system, which is unsaturated.
- Representative examples of cycloalkenyl include, but are not limited to, cyclopropenyl, cyclobutenyl, cyclopentenyl and cyclohexenyl.
- alkenylaryl and “alkynylaryl” refers to alkeny and alkynyl group, respectively, are further substituted with an aryl group.
- alkenylheteroaryl and “alkynylheteroaryl” refers to akenyl chain is substituted with a heteroaryl group.
- aryl as used herein, include aromatic mono or bi-cyclic compound having 5-10 atoms which may be optionally fused with a cycloalkyl ring.
- aryl group are phenyl and naphthyl.
- heteroaryl refers to an aryl group containing 1-4 heteroatoms. Unless specifically specified, the heteroatom is selected from N, O and S.
- the non-limiting examples of heteroaryl group are oxazolyl, isoxazolyl, imidazolyl, furyl, pyrrolyl, triazolyl, triazinyl, tetrazoyl, thienyl, thiazolyl, isothiazolyl, pyridyl, pyrimidinyl, pyrazinyl, pyridazinyl, benzofuranyl, benzothiazolyl, benzoxazolyl, benzimidazolyl, benzothienyl, benzopyranyl, quinolinyl, isoquinolinyl, quinazolinyl, pyrazolo[3,4-(f]pyrimidin and pyrrolo [2 , 3 -d] pyrimidin .
- heterocyclic or “heterocyclyl” unless otherwise specified refers to substituted or unsubstituted non-aromatic 3 to 15 membered ring radical which consists of carbon atoms and from one to five heteroatoms selected from nitrogen, oxygen and sulfur.
- the heterocyclic ring radical may be a mono-, bi- or tricyclic ring system, which may include fused, bridged or spiro ring systems, and the nitrogen, phosphorus, carbon, oxygen or sulfur atoms in the heterocyclic ring radical may be optionally oxidized to various oxidation states.
- the nitrogen atom may be optionally quarternized; also, unless otherwise constrained by the definition the heterocyclic ring or heterocyclyl may optionally contain one or more olefinic bond(s) thus forming heterocycloalkenyl group.
- heterocyclic ring radicals include, but are not limited to azepinyl, azetidinyl, benzodioxolyl, benzodioxanyl, chromanyl, dioxolanyl, dioxaphospholanyl, isoxazolidinyl, morpholinyl, oxazolinyl, oxazolidinyl, oxadiazolyl, 2-oxopiperazinyl, 2- oxopiperidinyl, 2-oxopyrrolidinyl, 2-oxoazepinyl, octahydroindolyl, octahydroisoindolyl, perhydroazepinyl, piperazinyl, 4-piperidonyl, pyrrolidinyl, piperidinyl, phenothiazinyl, phenoxazinyl, quinuclidinyl, tetrahydroisquino
- heterocyclic ring radical may be attached to the main structure at any heteroatom or carbon atom that results in the creation of a stable structure. Unless set forth or recited to the contrary, all heterocyclyl groups described or claimed herein may be substituted or unsubstituted.
- heterocycloalkenylalkyl refers to alky chain substituted with a heterocycloalkenyl ring.
- the present invention provides a compound of Formula I and pharmaceutically acceptable salts thereof
- Ri is selected from a group consisting of hydrogen, -C 1 -4 alkyl, -C 3 alkenyl, -C 3 alkynyl, -Q_ 4 haloalkyl, -C2-4 haloalkenyl, -C 3 haloalkylalkynyl, -C 1 -4 cyanoalkyl, -C2-4 cyanoalkenyl, -C2 cyanoalkynyl, -C2-5 O-alkyl, -C2-5 O-haloalkyl, -Cj,.(, cycloalkyl, -C 4 _6 heterocycloalkyl containing 1-3 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C 1 -4 alkyl or -CO-Ci_ 4 alkyl group; and when Rj is alkenyl or alkynyl, the double and triple bond, respectively, is not on the
- R2 is selected from hydrogen, -Ci_ 4 alkyl, -C 3 alkenyl, -C 3 _ 4 alkynyl, -C 1 -4 haloalkyl, -C2-A haloalkenyl, -C 3 _ 4 haloalkylalkynyl, -C 1 -4 cyanoalkyl, -C2-4 cyanoalkenyl, -C2 cyanoalkynyl, - C2-5 O-alkyl, -C 2 - 5 O-haloalkyl, -C 3 - 6 cycloalkyl, -CN, -C(0)R 5 , -C(S)R 5 , -S0 2 R 5 , -SOR5, - CONHSO2R5, -CSNHSO2R5, -CONHCOR5, -CSNHCOR5, -CON(d_ 4 alkyl)COR 5 , - C(S)N(Ci-4
- Rs and R are independently selected from hydrogen, d-6 alkyl, -6 alkenyl, C 3 - 7 cycloalkyl or Rg and R together forms 4 to 10 membered mono or bicyclic carbocyclic ring optionally substituted with one or more d_ 3 alkyl group or a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C 1 -4 alkyl or -CO-d_4 alkyl group;
- EWG is an electron withdrawing group selected from -COOR 10 , -CON(R J O) 2 , -CN, -N0 2 , S0 3 H, -S0 2 N(R J O) 2 , -S0 2 Rio wherein Rjo is selected from hydrogen, -d_ 3 alkyl and -C 4 cycloalkyl;
- T is a heteroatom selected from oxygen, nitrogen, or sulfur and when the heteroatom nitrogen it is optionally further substituted with -d-4 alkyl or -CO-d_ 4 alkyl group; p is an integer selected from 1 and 2;
- Rj3 and R 14 are independently selected from hydrogen, Ci_6 alkyl, C 2 _6 alkenyl, C3_7 cycloalkyl or Ri 3 and Ri 4 together forms 4 to 10 membered mono or bicyclic carbocyclic ring or a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -Ci_ 4 alkyl or -CO-C1 alkyl group;
- EWG 2 is an electron withdrawing group selected from -COOR 1 5, -CON(R 15 ) 2 , -CN, -N0 2 , - SO 3 H, -S0 2 N(Ri5) 2 , -S0 2 Ri5 wherein R15 at each occurrence is independently selected from hydrogen, -Ci_3 alkyl and -C 4 _6 cycloalkyl; q is an integer selected from 1 and 2;
- R3 and R 4 are independently selected from hydrogen, halogen, Ci_6 alkyl and C3_6 cycloalkyl;
- ring P is 4 to 13 membered saturated or unsaturated monocyclic, bicyclic or tricyclic ring optionally containing one additional heteroatom selected from oxygen, nitrogen and sulfur;
- ring A is a heterocycle selected from moieties provided in Figure 3
- Q is selected from CH or nitrogen
- Rj6 and R 17 together forms a phenyl ring optionally substituted with halogen, -0-Ci_3 alkyl, - COOH, -COOd_ 3 alkyl, -C 1-3 alkyl, -OH; or Rj6 and R 17 together forms a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen and sulfur; V independently selected from R 18 , halogen, -C 1 -4 haloalkyl, -C2-4 alkenyl, -C2 alkynyl, - SR18, -OR18, -SORig, -SO2R18, -N(R 18 ) 2 , -NHCHO, -NHCOCH3, -C 4 - 6 hetrocycloalkyl, -C 4 _ 6 cycloalkenyl, -CO-Ris, -CN and -CHO wherein Rjg at each occurrence is independently selected from hydrogen,
- R 19 is selected from hydrogen, halogen, hydroxyl, -N(Ci_6 alkyl)2, -NH(Ci_6 alkyl), - Cj_6 haloalkyl, -C 2- 6 alkenyl, -C 2-6 alkynyl, -Od-6 alkyl, -O d-6 alkenyl, -O d-6 alkynyl,-S- Ci-6 alkyl, -CN, -CO-C1-4 alkyl, -CO— C3-6 cycloalkyl, -COOH, -CONH 2 , -CON(d-6 alkyl) 2 , - CONH(d_6 alkyl), -NHC(O) -d-4 alkyl, -N(C 1 alkyl)C(O) d-4 alkyl and when R 19 is -OC 3 - 6 alkenyl or -O d-6 alkynyl the double and triple bond, respectively, is not
- Yi, Y 2 , Y3 and Y 4 are independently and appropriately selected from CH, NR2 0 , O, or S; wherein, R20 is selected from hydrogen, -C 1 -3 alkyl and -d-6 cycloalkyl;
- r is an integer selected from 1 to 3;
- D is a ring containing 5 to 13 membered aryl, fused aryl, heteroaryl, fused heteroaryl, saturated or unsaturated monocyclic, bicyclic or tricyclic carbocyclic ring containing 0-3 heteroatoms selected from nitrogen, oxygen and sulfur; optionally substituted with d_3 alkyl; or D is selected from -d-6 alkyl, -C3-6 cycloalkyl, -C1-4 alkyl-O-d ⁇ alkyl, -C3-6 cyclolkyl-O- d- 4 alkyl, -C1-4 alkyl-0-C3_6 cycloalkyl, -d_ 4 haloalkyl-O-d ⁇ alkyl, -C1-4 haloalkyl-S-d-4 alkyl and -C 1 -4 alkyl-S-C2- 4 alkyl; with the proviso that when:
- ring B and D are phenyl
- Z is selected from -0-, -S-, -NH-, -N(d_4 alkyl)- or -C(O)-;
- Ring A is selected from
- R 2 is not selected from -C(0)R 5 , -C(S)R 5 , -S0 2 R 5 , -SOR 5 wherein R 5 is R y ;
- the present invention provides a compound of Formula I; wherein
- Rj is hydrogen
- R 2 is selected from -CN, -C(0)R 5 , -C(S)R 5 , -S0 2 R 5 wherein,
- R5 is selected from a group comprising Ry, -Ci_6 alkyl, -C3-6 cycloalkyl, -Ci_3 heterocycloalkenylalkyl, NH C6-10 aryl, Cs_io heteroaryl containing 1-3 heteroatoms selected from oxygen, nitrogen or sulfur, wherein
- alkyl and cycloalkyl are optionally further substituted with CN group; and aryl, heteroaryl and heterocycloalkenylalkyl are optionally substituted with one or more halogen;
- n is an integer and at each occurrence is selected from 0 to 3;
- R 6 is selected from hydrogen, -Ci_3 alkyl; or R 2 is a 6 membered heteroaryl ring substituted with at least one group selected from halogen, C3-6 alkenyl, C3-6 alkynyl and -SH group; and optionally further substituted with one or more groups selected from S-C 2 ⁇ alkenyl, -COOH, -OCi_ 6 alkyl, -CON(-Ci_ 3 alkyl), -N0 2 and -CONH 2 ; or R 2 is a group selected from following moieties
- Rs and R9 are independently selected from hydrogen and Ci-6 alkyl, or Rs and R9 together forms 4 to 10 membered mono-cyclic ring optionally substituted with one or more d_3 alkyl group;
- EWG is-C(0)Od_ 3 alkyl
- Rn and Rj2 are independently selected from hydrogen, Ci_6 alkyl, C2-6 alkenyl, - COOR and -C 4 _6 heteroaryl containing 1-3 heteroatoms selected from oxygen, nitrogen or sulfur; wherein R is hydrogen or Ci_6 alkyl and the heteroaryl is optionally substituted with one or more halogen atoms; or Rn and Rj2 together forms substituted or unsubstituted 5 to 6 membered saturated or unsaturated mono carbocyclic ring or a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen and nitrogen; and the carbocyclic and the heterocyclic rings are optionally further substituted with one or more halogen atoms;
- ring P is 4 to 6 membered saturated monocyclic, ring containing one additional heteroatom selected from nitrogen;
- ring A is a heterocycle selected from followin moieties
- Q is selected from CH or nitrogen
- ring B is phenyl
- Z is selected from -0-, -C(0)NH- and -NHC(O)-;
- D is phenyl or pyridine ring unsubstituted or substituted with Ci_3 alkyl.
- the present invention provides a compound of Formula I, wherein Rj is hydrogen; R2 is -C(0)Rs or a 6 membered heteroaryl ring substituted with one or more halogen radical;
- R2 is a group selected from following moieties wherein, Rg and R are independently selected from hydrogen and Ci_6 alkyl, or Rg and R together forms 4 to 10 membered mono-cyclic ring optionally substituted with one or more Ci-3 alkyl group;
- EWG is-C(0)Od_ 3 alkyl
- ring A is a heterocycle selected from following moieties.
- the compounds of the present invention can be used as selective agents as inhibitors of BTK either alone or in combination with other related kinases inhibitors, or any other appropriate drugs.
- the compound of present invention finds use in treating cancer, particularly in haematological malignancies such as chronic lymphocytic leukaemia (CLL), mantle cell lymphoma (MCL), follicular lymphoma (FL) or even disorder such as diffused large B-cell lymphoma (DLBCL) etc., in which the activated B-cell need to be controlled or the proliferation needs to be down regulated.
- CLL chronic lymphocytic leukaemia
- MCL mantle cell lymphoma
- FL follicular lymphoma
- DLBCL diffused large B-cell lymphoma
- the compounds of present invention can be prepared by any of the following me
- PG represents an amino protecting group such as a Boc, Fmoc or CBZ which can be cleaved easily under acidic, basic or neutral conditions to the substituted hydrazines of interest.
- L is leaving group such as halogen or a alkyl-sulfonate.
- cyclic amines represented by (i) are treated with alkali nitrites under acidic condition or by the use of an alkyl nitrite, to obtain the nitroso derivatives such as (ii), which on further reduction results in some of the hydrazines of interest as in (iii).
- Hydrazines (iii) can also be prepared by direct displacement of a leaving group (LG) on nitrogen such as mesylate, tosylate, brosylate, triflates or nonaflates to give protected hydrazino derivatives such as (iv) which on deprotection render compounds represented by (iii) as depicted in Method B
- hydrazines are prepared by treatment of acyclic compounds such as (v) with mono-N-protected hydrazides to obtained directly the cyclic product represented by (iv) which on de -protection under suitable conditions result in required hydrazines (iii).
- the hydrazines of Formula iii can be converted into compound of Formula I by reacting them
- LG-R and/or LG-R groups wherein LG is a leaving group are optionally substituted with LG-R and/or LG-R groups wherein LG is a leaving group.
- 'A' ring as a pyrazinopyrimidine can be prepared by a sequence starting with condensation of melanonitrile with properly substituted acid chlorides and the condensation product on treatment with hydrazines can give the cyclized amino-pyrazoles as shown.
- pyrazolo compound on treatment with formaldehyde and ammonia results in the central A ring of requirement.
- 'A' ring as a starting material can be elaborated to the required hydrazines by functional transformations as outlined in Scheme-3.
- 'L' can be a hydroxyl group which participate in Mitsunobu reaction to form C-N bond.
- the halogenated derivative can further be elaborated by treating with a boronate under Suzuki coupling condition to obtain the skeletons of the requirement which can be transformed to the hydrazines of choice.
- 'A' ring is an imidazole -pyrazine envisioned can be synthesized as er the brief Scheme-4 outlined hereunder.
- a commercially available 2-chloro-3-cyanopyrazole can be reduced either by known hydrogenation method or by involving reducing agents such as lithium aluminium hydride to convert into an amine which can be treated with an acid chloride or appropriately activated acid to form an amide bond as shown in Scheme-4.
- the amide intermediate can be ring closed to obtain the 'A' ring as an imidazole-pyrazine scaffold of choice.
- 'A' ring can be modified as pyrazolo- pyrimidine or pyrazolo-pyridine scaffolds which are differently disposed from the earlier described embodiments.
- chlorofluropyrimidine or chlorofluropyridine can be acylated with suitable acid chloride moiety and then treated with hydrazine to form the scaffold of interest. Further elaboration can result in some of the hydrazines of current interest.
- the 'A' ring scaffold can be modified to a 2-imidazalone fused
- Bz in Scheme-6 is a benzyl group which may be substituted of unsubstituted.
- the A-ring can be synthesized by successive substitutions with different amines of choice followed by the reduction of the nitro group to obtain a 1,2- diamino function which can be ring closed to form the urea linkage by either CDI or 4- nitrophenyl chloroformate.
- These intermediates can be further elaborated by a Suzuki or by Buchwald coupling methods to obtain the needed disposition of groups and the hydrazines of choice.
- the linkage of the groups is not confined to one of the fused heterocyclic rings such as pyrazole or imidazole but can be viewed as a substituents attached in both the rings as given in some of the scaffolds of Figure-3.
- Scheme-7 given below outlines the synthesis of such hyrdrazines.
- substituted dichloropurine can be functionalized to obtain the required hydrazine in few steps.
- Hydrazines prepared as per the above methods described in Scheme- 1 to Scheme-7 can be treated with suitable electrophilic compounds to afford the compounds of Formula I.
- Some of the representative electrophilic compounds are provided in Figure-5.
- the electrophilic compound may require some functional modifications depending upon the type .
- Ar in Figure-5 is an aryl group and Het.Ar is a heteroaryl group.
- R a and R are independently H, alkyl Ci_6, cycloalkyl Cj,.(,-
- the hydrazines prepared as per the process provided in Scheme- 1 to Scheme-7 were treated with aromatic electrophiles.
- Some of the representative aromatic electrophilic compounds are provided in Figure-6.
- the aromatic electrophilic compound may also require some functional modifications depending upon the type of the substitution on them and such modifications are under the purview of a person skilled in the art.
- the hydrazines prepared according to the methods described above can be treated with substrates containing aldehydes and ketones containing either saturated or unsaturated functionalities as condensing agents to obtain the compound of Formula I.
- substrates containing aldehydes and ketones containing either saturated or unsaturated functionalities as condensing agents to obtain the compound of Formula I.
- Representative aldehydes and ketones are provided in Figure-7.
- hydrazines prepared according to the methods described above can be treated with 1,3 diketones, 1,3 ketoesters or 1,2 ketoesters to afford compound of Formula I having ene- amide, ene-ester etc. linked substitution.
- the representative 1,3 diketones, 1,3 ketoesters or 1,2 ketoesters are provided in Figure-8
- Example 1 fert-Butyl N-(4-hvdroxy-l-piperidyl)carbamate
- THF 100 mL
- RB bottom
- triethylamine 16.4 mL
- Boc 21.4 mL
- the content of the flask was concentrate under reduced pressure. Water was added to the residue and the mixture was extracted with ethyl acetate. The organic layer was dried over anhydrous sodium sulfate and concentrated. The residue was subjected to column chromatography to afford the title compound as an off-white solid.
- Example 5 l-Nitrosopiperidin-4-ol To a cold (0-10 °C) and stirred solution of 4-hydroxypiperidine (50 g, 0.49 mole) in a mixture of 300 ml water and acetic acid (45 g, 0.741 mole), sodium nitrite (68.2 g, 0.99 mole) was added and the mixture was stirred for 1-2 h at ambient temperature. The reaction mixture was quenched with sodium carbonate extracted with ethyl acetate and the organic layer was concentrated to obtain title compound as off white solid.
- 4-hydroxypiperidine 50 g, 0.49 mole
- acetic acid 45 g, 0.741 mole
- sodium nitrite 68.2 g, 0.99 mole
- Example 8 jV-(4-Methyl-pyridin-2-yl)-4-(4,4,5,5-tetramethviri,3,21dioxaborolan-2-yl)- benzamide (8-6)
- Example 15 l-Cyano-cyclopropanecarboxylic acid ⁇ 4-r4-amino-3-(4-phenoxy-phenyl)- Pyrazolor3,4- ⁇ flpyrimidin-l-vnpiperidine-l-yl)-amide 1.11:
- Example 18 4-ri-(l-Acryloylamino-piperidine-4-yl)-4-amino-iH-pyrazolo-r3,4- ⁇ flpyrimidine-3-vn-jV-(5-methyl-pyridin-2-yl)-benzamide 1.33
- N,N-Dibenzyl-6-chloro-5-nitro-pyrimidin-4-amine (18g) and teri-butyl 4-aminopiperidine-l- carboxylate (15.15 g) were dissolved in dioxane (90.0 mL). Triethylamine (10.5 mL) was added and the mixture was stirred for 16 hours at 60 °C. The reaction mixture was cooled to room temperature and the solvent was distilled off. Residue was extracted with ethyl acetate and the organic layer was washed with water, saturated aqueous sodium chloride solution and dried over anhydrous sodium sulfate.
- Example 21 fert-Butyl 4-rr5-amino-6-(dibenzylamino)pyrimidin-4-vnamino1piperidine- 1-carboxylate (9.4)
- Example 26 6-Amino-9-(l-nitroso-4-piperidyl)-7-(4-phenoxyphenyl)purin-8-one (9.9)
- a solution of sodium nitrite (0.42 g) in water (10 mL) was added drop-wise into a solution of compound 9.8 (1.0 g) in acetic acid (15.0 mL) at low temperature (-5 to 0 C) and stirred at room temperature for 6 hours.
- the solvent was distilled off from the reaction mixture, water was added, and the extraction with ethyl acetate was performed.
- the organic layer was washed with saturated aqueous sodium chloride solution then dried over anhydrous sodium sulfate, and the solvent was distilled off.
- the residue was purified by silica gel column chromatography to obtain the title compound (0.8 g) as an off-white solid.
- Example 28 N- ⁇ 4- ⁇ 6- Amino-8-oxo-7-(4-phenoxyphenyl)purin-9- yll - 1 -piperidyll -3- (2,5- dioxopyrrol-l-yl)propanamide (1.39)
- Example 35 7-(l-amino-4-piperidyl)-2-chloro-5-(4-phenoxyphenyl)pyrrolor2,3- ⁇ flpyrimidin-4-amine (10.8)
- Example 36 ⁇ V-[4-f4-amino-2-chloro-5-(4-phenoxyphenyl)pyrrolo[2,3- ⁇ /
- BTK (h) was incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA. 250 ⁇ VEKJGEGTYG V V YK (Cdc2 peptide), 10 mM MgAcetate and [ ⁇ -33 ⁇ - ⁇ ] (specific activity approx. 500 cprn/pmol, concentration as required).
- the reaction was initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction was stopped by the addition of 3 % phosphoric acid solution. 1 0 ⁇ . of the reaction was then spotted onto a P30 filtermat and washed three times for 5 minutes with 75 mM phosphoric acid and once with methanol prior to drying and scintillation counting.
- the compounds of present invention showed more than 50 % inhibition of BTK at 100 nM.
- Table 2 provides percentage inhibition at 10 nM and 100 nM for few representative compounds in radioligand binding assay.
Abstract
The present invention relates to novel hydrazino compounds of Formula (I) as Bruton tyrosine kinase inhibitors, process of preparation thereof, and to the use of the compounds in the preparation of pharmaceutical compositions for the therapeutic treatment of disorders involving mediation of Bruton tyrosine kinase in humans.
Description
NOVEL HYDRAZINO COMPOUNDS AS BTK INHIBITORS
RELATED APPLICATIONS This application claims the benefit of Indian Patent Application no. IN 201621003737 filed on February 02, 2016 which is hereby incorporated by reference.
FIELD OF THE INVENTION The present invention relates to novel hydrazino compounds as Bruton tyrosine kinase (BTK) inhibitors, process of preparation thereof, and to the use of the compounds in the preparation of pharmaceutical compositions for the therapeutic treatment of disorders involving mediation of Bruton tyrosine kinase in humans. BACKGROUND OF THE INVENTION
Involvement of BTK in signal transduction pathways regulating B-cell proliferation, differentiation and survival has been a ground breaking discovery and has led to developments of drugs for the treatment of B-cell malignancies. Improper /auto activated BTK signaling is considered to be the major cause in several haematological malignancies such as chronic lymphocytic leukemia (CLL), mantle cell lymphoma (MCL) etc. (Li et al. Journal of Med. Chem., 2014, 57(12), 5112-5128; Seng-Lai Tan et al., Pharmacology and Therapeutics, 2013, 138, 294-309). First in class BTK inhibitor, Ibrutinib, which is disclosed in US patent number 7514444 is presently marketed for the treatment of mantle cell lymphoma and chronic lymphocytic leukemia under the trade name Imbruvica . The clinical and commercial success of Ibrutinib has led to discovery of newer BTK inhibitors. WIPO publication number WO 2015002894A1, WO 2014188173A1, WO 2014188173A1, WO 2014100748A1, WO2011031896, WO2013155347, WO2014130856, WO2015165279A1 ; US patent numbers 7169791, 6596746, 6002008, 8114874, 7728131 and US publication US 2015 0005277, US20140079690 discloses various compounds as BTK inhibitors.
The present inventors have found novel hydrazine compounds which have shown potent BTK inhibitory activity.
SUMMARY OF THE INVENTION
The present invention provides a compound of Formula I and pharmaceutically acceptable salts thereof
wherein, Rj is selected from a group consisting of hydrogen, -C1-4 alkyl, -C3 alkenyl, -C3 alkynyl, -C1-4 haloalkyl, -C2-4 haloalkenyl, -C3 haloalkylalkynyl, -C1-4 cyanoalkyl, -C2-4 cyanoalkenyl, -C2 cyanoalkynyl, -C2-5 O-alkyl, -C2-5 O-haloalkyl, -Cj,.(, cycloalkyl, -C4_6 heterocycloalkyl containing 1-3 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C1-4 alkyl or -CO-Q_ 4 alkyl group; and when Rj is alkenyl or alkynyl, the double and triple bond, respectively, is not on the carbon directly attached to the nitrogen atom;
R2 is selected from hydrogen, -Ci_4 alkyl, -C3 alkenyl, -C3_4 alkynyl, -C1-4 haloalkyl, -C2 haloalkenyl, -C3_4 haloalkylalkynyl, -C1-4 cyanoalkyl, -C2-4 cyanoalkenyl, -C2 cyanoalkynyl, - C2-5 O-alkyl, -C2-5 O-haloalkyl, -C3-6 cycloalkyl, -CN, -C(0)R5, -C(S)R5, -S02R5, -SOR5, - CONHSO2R5, -CSNHSO2R5, -CONHCOR5, -CSNHCOR5, -CON(d_4 alkyl)COR5, , - C(S)N(Ci-4 alkyl)COR5, -CON(d_4 alkyl)S02R5, -CSN(Ci_4 alkyl)S02R5 -C(=NR6)R5, - C(=NR6)N(R6)C(0)R5, -C4_6 heterocycloalkyl containing 1-3 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C1-4 alkyl and -CO-Ci_4 alkyl group, and when R2 is alkenyl or alkynyl, the double and triple bond, respectively, is not on the carbon directly attached to the nitrogen atom; wherein R5 is selected from a group comprising Ry, hydrogen, -NH2, -NHOH, -NRi(Ci_4 alkyl)-, -Ci_6 alkyl, -C3-6 cycloalkyl, -Ci_6 haloalkyl, -C3-6 cycloalkenyl, -Ci_3
heterocycloalkenylalkyl, -C3_6 heterocycloalkyl, -NH C6-10 aryl, _io heteroaryl containing 1- 3 heteroatoms selected from oxygen, nitrogen or sulfur; wherein alkyl, cycloalkenyl, cycloalkyl are optionally further substituted with CN group; and aryl, heteroaryl and heterocycloalkenylalkyl are optionally substituted with one or more halogen; and Rs> is selected from a group consisting of -C2-6 alkenyl, -C2-6 alkynyl, -C3_6 alkenylaryl, -C3_6 alkenylheteroaryl, -C3_6 alkynylaryl, -C3_6 alkynylheteroaryl, -CH=CH-(CH2)n-COOR6, - C(=CH2)CH2COOR6, -C≡C-(CH2)n-COOR6, -CH=CH-(CH2)n-CN, -C≡C-(CH2)n-CN, - CH=CH-(CH2)n-OR6, -C≡C-(CH2)n-OR6, -CH=CH-(CH2)n-NR6R7 and -C≡C-(CH2)n-NR6R7; n is an integer and at each occurrence is selected from 0 to 3; m is an integer and at each occurrence is selected from 1 to 3; R6 and R7 at each occurrence are independently selected from hydrogen, -Ci_3 alkyl and -C4_6 cycloalkyl or R6 and R7 together forms a 4-6 membered ring optionally containing one additional heteroatom selected from oxygen, nitrogen, or sulfur; or R2 is an aryl group substituted with at least one halogen atom and at least one group at ortho or para position with respect to halogen atom selected from -CN, -COOH, -COO-Ci_3 alkyl, -CON(-d_3 alkyl), -N02, -S02d_3 alkyl, -S02NH2, -S02NH-d_3 alkyl, -S02N(d_3 alkyl)2, -CO-d_3 alkyl, -CONH2, -CONH(d_3 alkyl), -CON(d_3 alkyl)2; or R2 is a 6 membered heteroaryl ring containing 1-3 nitrogen atoms or 5 membered ring containing 1 to 3 heteroatoms selected from oxygen, nitrogen, or sulfur wherein the 6 membered or 5 membered ring is substituted with at least one group selected from halogen, _6 alkenyl, _6 alkynyl, -COOH and -SH group; and optionally further substituted with one or more groups selected from -CN, S-C2-4 alkenyl, -COOH, -OCi-6 alkyl, -COO-C1-3 alkyl, - CON(-d_3 alkyl), -N02, -S02d_3 alkyl, -S02NH2, -S02NH-d_3 alkyl, -S02N(d_3 alkyl)2, - CO-d-3 alkyl, -CONH2, -CONH(d-3 alkyl), -CON(d-3 alkyl)2; or R2 is a group selected from moieties provided in Figure 1
Figure 1
wherein, Rs and R are independently selected from hydrogen, Ci-6 alkyl, C2-6 alkenyl, C3-7 cycloalkyl or Rg and R together forms 4 to 10 membered mono or bicyclic carbocyclic ring optionally substituted with one or more Ci_3 alkyl group or a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C1-4 alkyl or -CO-C1-4 alkyl group;
EWG is an electron withdrawing group selected from -COOR10, -CON(Rio)2, -CN, -NO2, - SO3H, -S02N(Rio)2, -SO2R10 wherein Rjo is selected from hydrogen, -Ci_3 alkyl and -C4_6 cycloalkyl;
T is a heteroatom selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -Ci_4 alkyl or -CO-Ci_4 alkyl group; p is an integer selected from 1 and 2;
' { ' is the point of attachment of R2 with nitrogen atom;
or
wherein, Rn and R12 are independently selected from hydrogen, Ci-6 alkyl, C2-6 alkenyl, C3-7 cycloalkyl, -COOR, -CON(R)2, -CH=CHCOOR, -CH=CHCONRR', -C4_6 heteroaryl containing 1-3 heteroatoms selected from oxygen, nitrogen or sulfur, the heteroaryl is optionally substituted with one or more halogen atoms; wherein R and R' are selected from
hydrogen or Ci_6 alkyl or R and R' together with the nitrogen to which they are attached forms a 5-6 membered saturated or unsaturated ring; or Rn and Rj2 together forms 4 to 10 membered mono or bicyclic saturated or unsaturated carbocyclic ring or a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen and sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C1-4 alkyl or
alkyl group; the carbocyclic and the heterocyclic rings are optionally further substituted with one or more halogen atoms; Ri3 and R14 are independently selected from hydrogen, Ci_6 alkyl, C2-6 alkenyl, C3_7 cycloalkyl or Rj3 and R14 together forms 4 to 10 membered mono or bicyclic carbocyclic ring or a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C1-4 alkyl or -CO-C1 alkyl group;
EWG2 is an electron withdrawing group selected from -COOR15, -CON(Rj5)2, -CN, -NO2, - SO3H, -S02N(Rj5)2, -SO2R15 wherein RJS at each occurrence is independently selected from hydrogen, -Ci_3 alkyl and -C4_6 cycloalkyl; q is an integer selected from 1 and 2;
' { ' is the point of attachment of Rj and R2 with nitrogen atom;
R3 and R4 are independently selected from hydrogen, halogen, Ci_6 alkyl and C3-6 cycloalkyl; ring P is 4 to 13 membered saturated or unsaturated monocyclic, bicyclic or tricyclic ring optionally containing one additional heteroatom selected from oxygen, nitrogen and sulfur; ring A is a heterocycle selected from moieties provided in Figure 3
Figure 3
wherein Q is selected from CH or nitrogen;
Rj6 and R17 together forms a phenyl ring optionally substituted with halogen, -0-Ci_3 alkyl, - COOH, -COOd_3 alkyl, -C1-3 alkyl, -OH; or Rj6 and R17 together forms a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen and sulfur; V independently selected from R18, halogen, -C1-4 haloalkyl, -C2-4 alkenyl, -C2 alkynyl, - SR18, -OR18, -SORig, -SO2R18, -N(R18)2, -NHCHO, -NHCOCH3, -C4-6 hetrocycloalkyl, -C4_6 cycloalkenyl, -CO-Ris, -CN and -CHO wherein Ris at each occurrence is independently selected from hydrogen, -Ci_4 alkyl and -C3_6 cycloalkyl; '{ ' represents the attachment to ring B;
'[' represents the attachment to ring P; ring B is sele
Figure 4
wherein, R19 is selected from hydrogen, halogen, hydroxyl, -N(Ci_6 alkyl)2, -NH(Ci_6 alkyl), - Cj_6 haloalkyl, -C2-6 alkenyl, -C2-6 alkynyl, -Od-6 alkyl, -O d-6 alkenyl, -O d-6 alkynyl,-S- Ci-6 alkyl, -CN, -CO-C1-4 alkyl, -CO— C3-6 cycloalkyl, -COOH, -CONH2, -CON(d-6 alkyl)2, - CONH(d_6 alkyl), -NHC(O) -d-4 alkyl, -N(C1 alkyl)C(O) d-4 alkyl and when R19 is -OC3-6 alkenyl or -O d-6 alkynyl the double and triple bond, respectively, is not on the carbon directly attached to the oxygen atom;
Yi, Y2, Y3 and Y4 are independently and appropriately selected from CH, NR20, O, or S; wherein, R20 is selected from hydrogen, -C1-3 alkyl and -d-6 cycloalkyl;
r is an integer selected from 1 to 3;
'{ ' represents the position of attachment to ring A;
'[' represents the position of attachment to Z;
Z is a bond or is selected from -0-, -S-, -NH-, -N(CM alkyl)-, -N(d-6 alkyl)CO-, -C(0)NH-, -C(d_2 alkyl)2-, -C(O)-, -S(O)-, -S(0)2-, -CH20-, -OCH2-, -CH=CH-, -CH2-, -N(CH2CN)- and -NHC(O)-;
D is a ring containing 5 to 13 membered aryl, fused aryl, heteroaryl, fused heteroaryl, saturated or unsaturated monocyclic, bicyclic or tricyclic carbocyclic ring containing 0-3 heteroatoms selected from nitrogen, oxygen and sulfur; optionally substituted with d_3 alkyl; or D is selected from -d-6 alkyl, -C3-6 cycloalkyl, -C1-4 alkyl-O-d^ alkyl, -C3-6 cyclolkyl-O- d-4 alkyl, -C1-4 alkyl-0-C3_6 cycloalkyl, -d_4 haloalkyl-O-d^ alkyl, -C1-4 haloalkyl-S-d-4 alkyl and -C1-4 alkyl-S-C2-4 alkyl; with the proviso that when:
ring B and D are phenyl;
Z is selected from -0-, -S-, -NH-, -N(d_4 alkyl)- or -C(O)-;
Ring A is selected from
and V is hydrogen then:
R2 is not selected from -C(0)R5, -C(S)R5, -S02R5, -SOR5 wherein R5 is Ry; and
Rj and R2 together does not form the following group
The compounds of the present invention were found to be potent and selective BTK inhibitors and can be useful in treatment of the diseases mediated by BTK receptors.
DEFINITION "Salts" according to the invention include those formed with either organic and inorganic acids or bases. Pharmaceutically acceptable acid addition salts include those formed from hydrochloric, hydrobromic, sulfuric, citric, tartaric, phosphoric, acetic, trifluoroacetic, triphenylacetic, phenylacetic, succinic, oxalic, fumaric, maleic, glutamic, aspartic, oxalacetic, methanesulphonic, ethanesulphonic, p-toluenesulphonic, benzenesulphonic, naphthalenesulphonic or naphthalenedisulphonic, salicylic, glutaric, gluconic, mandelic, cinnamic, ascorbic, oleic, naphthoic, hydroxynaphthoic (for example 1- or 3-hydroxy-2- naphthoic), benzoic, 4-methoxybenzoic, 2- or 4-hydroxybenzoic, 4-chlorobenzoic, 4- phenylbenzoic acids and butane- 1,4-disulfonic acid. Pharmaceutically acceptable base salts include ammonium salts, alkali metal salts such as those of sodium and potassium, alkaline earth metal salts such as those of calcium and magnesium and salts with organic bases such as dicyclohexylamine and N-methyl-D-glucamine.
The term "alkyl" refers to a saturated hydrocarbon chain radical that includes solely carbon and hydrogen atoms in the backbone, either linear or branched and which is attached to the rest of the molecule by a single bond, e.g., methyl, ethyl, w-propyl, 1-methylethyl (isopropyl), «-butyl, «-pentyl, and 1,1-dimethylethyl (i-butyl). The alkyl chain may have 1 to 13 carbon atoms unless specified otherwise. Unless set forth or recited to the contrary, all alkyl groups described or claimed herein may be unsubstituted or substituted with groups selected from halogen, -Od-6 alkyl, -OC3-6 cycloalkyl, CN, -COOH, -COO-d-3 alkyl, -CON(-d-3 alkyl), - N02, -S02d_3 alkyl, -S02N-d_3 alkyl, -CO-d_3 alkyl.
The numerical in phrases like "C1-4 alkyl", refers that there are 1 to 4 carbon atoms in the alky chain.
The term "alkenyl" refers to a hydrocarbon chain containing at least one carbon-carbon double bond, and may have (E) or (Z) configuration. An alkenyl group may contain 2 to 8 carbon atoms unless specified otherwise. Non-limiting examples of alkenyl groups include 2- propenyl (allyl), 2-methyl-2-propenyl, and (Z)-2-butenyl. Unless set forth or recited to the contrary, all alkenyl groups described or claimed herein may be straight chain or branched, unsubstituted or substituted with groups selected from halogen, -OCi-6 alkyl, -OC3-6 cycloalkyl, CN, -COOH, -COO-d_3 alkyl, -CON(-d_3 alkyl), -N02, -S02d_3 alkyl, -S02N- d_3 alkyl, -CO-d_3 alkyl.
The term "alkynyl" refers to a hydrocarbon chain having at least one carbon-carbon triple bond. An alkynyl group may contain 2 to 8 carbon atoms unless specified otherwise. Non- limiting examples of alkynyl groups include 2-propynyl and 3-butynyl. Unless set forth or recited to the contrary all alkynyl groups described or claimed herein may form part of a straight or branched, substituted or unsubstituted chains. The alkynyl group may be unsubstituted or substituted with the groups selected from halogen, -Od_6 alkyl, -Od_6 cycloalkyl, CN, -COOH, -COO-d_3 alkyl, -CON(-d_3 alkyl), -N02, -S02d_3 alkyl, -S02N- Ci-3 alkyl, -CO-d_3 alkyl.
The term "halo", as used herein includes chloro, fluoro, bromo and iodo. Similarly the terms "haloalkyl", "haloalkeny and "haloalkyny refers to halo substituted alkyl, alkenyl and alkynyl chain, respectively.
The term "cycloalkyl" denotes a non-aromatic mono-, or multicyclic ring system of 3 to about 13 carbon atoms. Monocyclic rings include, but are not limited to cylcopropyl, cyclobutyl, cyclopentyl and cyclohexyl. Examples of simple multicyclic cycloalkyl groups include perhydronapththyl, perhydroindenyl etc; bridged multicyclic groups include adamantyl and norbornyl etc, and spriromulticyclic groups for e.g., spiro(4,4)non-2-yl. Unless set forth or recited to the contrary, all cycloalkyl groups described or claimed herein may be substituted or unsubstituted.
The term "heterocycloalkyl" refers to a cycloalkyl ring containing a heteroatom. Unless specifically specified, the heteroatom is selected from N, O and S.
The term "cycloalkenyl" refers to a monocyclic or bicyclic, 3 to 14 membered ring system, which is unsaturated. Representative examples of cycloalkenyl include, but are not limited to, cyclopropenyl, cyclobutenyl, cyclopentenyl and cyclohexenyl.
The terms "alkenylaryl" and "alkynylaryl" refers to alkeny and alkynyl group, respectively, are further substituted with an aryl group. Similarly, "alkenylheteroaryl" and "alkynylheteroaryl" refers to akenyl chain is substituted with a heteroaryl group.
The term "aryl" as used herein, include aromatic mono or bi-cyclic compound having 5-10 atoms which may be optionally fused with a cycloalkyl ring. The non-limiting examples of aryl group are phenyl and naphthyl.
The term "heteroaryl" as used herein, refers to an aryl group containing 1-4 heteroatoms. Unless specifically specified, the heteroatom is selected from N, O and S. The non-limiting examples of heteroaryl group are oxazolyl, isoxazolyl, imidazolyl, furyl, pyrrolyl, triazolyl, triazinyl, tetrazoyl, thienyl, thiazolyl, isothiazolyl, pyridyl, pyrimidinyl, pyrazinyl, pyridazinyl, benzofuranyl, benzothiazolyl, benzoxazolyl, benzimidazolyl, benzothienyl, benzopyranyl, quinolinyl, isoquinolinyl, quinazolinyl, pyrazolo[3,4-(f]pyrimidin and pyrrolo [2 , 3 -d] pyrimidin .
The term "heterocyclic " or "heterocyclyl" unless otherwise specified refers to substituted or unsubstituted non-aromatic 3 to 15 membered ring radical which consists of carbon atoms and from one to five heteroatoms selected from nitrogen, oxygen and sulfur. The heterocyclic ring radical may be a mono-, bi- or tricyclic ring system, which may include fused, bridged or spiro ring systems, and the nitrogen, phosphorus, carbon, oxygen or sulfur atoms in the heterocyclic ring radical may be optionally oxidized to various oxidation states. In addition, the nitrogen atom may be optionally quarternized; also, unless otherwise constrained by the definition the heterocyclic ring or heterocyclyl may optionally contain one or more olefinic bond(s) thus forming heterocycloalkenyl group. Examples of such heterocyclic ring radicals include, but are not limited to azepinyl, azetidinyl, benzodioxolyl, benzodioxanyl, chromanyl, dioxolanyl, dioxaphospholanyl, isoxazolidinyl, morpholinyl, oxazolinyl, oxazolidinyl,
oxadiazolyl, 2-oxopiperazinyl, 2- oxopiperidinyl, 2-oxopyrrolidinyl, 2-oxoazepinyl, octahydroindolyl, octahydroisoindolyl, perhydroazepinyl, piperazinyl, 4-piperidonyl, pyrrolidinyl, piperidinyl, phenothiazinyl, phenoxazinyl, quinuclidinyl, tetrahydroisquinolyl, tetrahydrofuryl, tetrahydropyranyl, thiazolinyl, thiazolidinyl, thiamo holinyl, thiamorpholinyl sulfoxide and thiamo holinyl sulfone. The heterocyclic ring radical may be attached to the main structure at any heteroatom or carbon atom that results in the creation of a stable structure. Unless set forth or recited to the contrary, all heterocyclyl groups described or claimed herein may be substituted or unsubstituted.
The term "heterocycloalkenylalkyl" refers to alky chain substituted with a heterocycloalkenyl ring.
DESCRIPTION
In one aspect, the present invention provides a compound of Formula I and pharmaceutically acceptable salts thereof
wherein, Ri is selected from a group consisting of hydrogen, -C1-4 alkyl, -C3 alkenyl, -C3 alkynyl, -Q_4 haloalkyl, -C2-4 haloalkenyl, -C3 haloalkylalkynyl, -C1-4 cyanoalkyl, -C2-4 cyanoalkenyl, -C2 cyanoalkynyl, -C2-5 O-alkyl, -C2-5 O-haloalkyl, -Cj,.(, cycloalkyl, -C4_6 heterocycloalkyl containing 1-3 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C1-4 alkyl or -CO-Ci_ 4 alkyl group; and when Rj is alkenyl or alkynyl, the double and triple bond, respectively, is not on the carbon directly attached to the nitrogen atom;
R2 is selected from hydrogen, -Ci_4 alkyl, -C3 alkenyl, -C3_4 alkynyl, -C1-4 haloalkyl, -C2-A haloalkenyl, -C3_4 haloalkylalkynyl, -C1-4 cyanoalkyl, -C2-4 cyanoalkenyl, -C2 cyanoalkynyl, - C2-5 O-alkyl, -C2-5 O-haloalkyl, -C3-6 cycloalkyl, -CN, -C(0)R5, -C(S)R5, -S02R5, -SOR5, - CONHSO2R5, -CSNHSO2R5, -CONHCOR5, -CSNHCOR5, -CON(d_4 alkyl)COR5, -
C(S)N(Ci-4 alkyl)COR5, -CON(d_4 alkyl)S02R5, -CSN(Ci_4 alkyl)S02R5 -C(=NR6)R5, - C(=NR6)N(R6)C(0)Rs, -C4_6 heterocycloalkyl containing 1-3 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C1-4 alkyl and -CO-Ci_4 alkyl group, and when R2 is alkenyl or alkynyl, the double and triple bond, respectively, is not on the carbon directly attached to the nitrogen atom; wherein R5 is selected from a group comprising Ry, hydrogen, -NH2, -NHOH, -NRi(Ci_4 alkyl)-, -Ci-6 alkyl, -C3-6 cycloalkyl, -Ci-6 haloalkyl, -C3-6 cycloalkenyl, -C1-3 heterocycloalkenylalkyl, -C3-6 heterocycloalkyl, -NH C6-10 aryl, _io heteroaryl containing 1- 3 heteroatoms selected from oxygen, nitrogen or sulfur; wherein alkyl, cycloalkenyl, cycloalkyl are optionally further substituted with CN group; and aryl, heteroaryl and heterocycloalkenylalkyl are optionally substituted with one or more halogen; and Rs> is selected from a group consisting of -C2-6 alkenyl, -C2-6 alkynyl, -C3-6 alkenylaryl, -C3_6 alkenylheteroaryl, -C3-6 alkynylaryl, -C3-6 alkynylheteroaryl, -CH=CH-(CH2)n-COOR6, - C(=CH2)CH2COOR6, -C≡C-(CH2)n-COOR6, -CH=CH-(CH2)n-CN, -C≡C-(CH2)n-CN, - CH=CH-(CH2)n-OR6, -C≡C-(CH2)n-OR6, -CH=CH-(CH2)n-NR6R7 and -C≡C-(CH2)n-NR6R7; n is an integer and at each occurrence is selected from 0 to 3; m is an integer and at each occurrence is selected from 1 to 3; R6 and R7 at each occurrence are independently selected from hydrogen, -C1-3 alkyl and -C4-6 cycloalkyl or R6 and R7 together forms a 4-6 membered ring optionally containing one additional heteroatom selected from oxygen, nitrogen, or sulfur; or R2 is an aryl group substituted with at least one halogen atom and at least one group at ortho or para position with respect to halogen atom selected from -CN, -COOH, -COO-Ci_3 alkyl, -CON(-d-3 alkyl), -N02, -S02d-3 alkyl, -S02NH2, -S02NH-d-3 alkyl, -S02N(d-3 alkyl)2, -CO-d_3 alkyl, -CONH2, -CONH(d_3 alkyl), -CON(d_3 alkyl)2; or R2 is a 6 membered heteroaryl ring containing 1-3 nitrogen atoms or 5 membered ring containing 1 to 3 heteroatoms selected from oxygen, nitrogen, or sulfur wherein the 6 membered or 5 membered ring is substituted with at least one group selected from halogen, _6 alkenyl, d-6 alkynyl, -COOH and -SH group; and optionally further substituted with one or more groups selected from -CN, S-C2_4 alkenyl, -COOH, -OCi_6 alkyl, -COO-Ci_3 alkyl, -
CON(-d_3 alkyl), -N02, -S02d-3 alkyl, -S02NH2, -S02NH-d-3 alkyl, -S02N(d-3 alkyl)2, - CO-d_3 alkyl, -CONH2, -CONH(d_3 alkyl), -CON(d_3 alkyl)2; or R2 is a group selecte
Figure 1
wherein, Rs and R are independently selected from hydrogen, d-6 alkyl, -6 alkenyl, C3-7 cycloalkyl or Rg and R together forms 4 to 10 membered mono or bicyclic carbocyclic ring optionally substituted with one or more d_3 alkyl group or a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C1-4 alkyl or -CO-d_4 alkyl group;
EWG is an electron withdrawing group selected from -COOR10, -CON(RJO)2, -CN, -N02, S03H, -S02N(RJO)2, -S02Rio wherein Rjo is selected from hydrogen, -d_3 alkyl and -C4 cycloalkyl;
T is a heteroatom selected from oxygen, nitrogen, or sulfur and when the heteroatom nitrogen it is optionally further substituted with -d-4 alkyl or -CO-d_4 alkyl group; p is an integer selected from 1 and 2;
' { ' is the point of attachment of R2 with nitrogen atom;
or
wherein, Rn and R12 are independently selected from hydrogen, Ci_6 alkyl, C2_6 alkenyl,
cycloalkyl, -COOR, -CON(R)2, -CH=CHCOOR, -CH=CHCONRR', -C4_6 heteroaryl containing 1-3 heteroatoms selected from oxygen, nitrogen or sulfur, the heteroaryl is optionally substituted with one or more halogen atoms; wherein R and R' are selected from hydrogen or Ci_6 alkyl or R and R' together with the nitrogen to which they are attached forms a 5-6 membered saturated or unsaturated ring; or Rn and R12 together forms 4 to 10 membered mono or bicyclic saturated or unsaturated carbocyclic ring or a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen and sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C1-4 alkyl or
alkyl group; the carbocyclic and the heterocyclic rings are optionally further substituted with one or more halogen atoms;
Rj3 and R14 are independently selected from hydrogen, Ci_6 alkyl, C2_6 alkenyl, C3_7 cycloalkyl or Ri3 and Ri4 together forms 4 to 10 membered mono or bicyclic carbocyclic ring or a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -Ci_4 alkyl or -CO-C1 alkyl group; EWG2 is an electron withdrawing group selected from -COOR15, -CON(R15)2, -CN, -N02, - SO3H, -S02N(Ri5)2, -S02Ri5 wherein R15 at each occurrence is independently selected from hydrogen, -Ci_3 alkyl and -C4_6 cycloalkyl; q is an integer selected from 1 and 2;
' { ' is the point of attachment of Rj and R2 with nitrogen atom;
R3 and R4 are independently selected from hydrogen, halogen, Ci_6 alkyl and C3_6 cycloalkyl; ring P is 4 to 13 membered saturated or unsaturated monocyclic, bicyclic or tricyclic ring optionally containing one additional heteroatom selected from oxygen, nitrogen and sulfur; ring A is a heterocycle selected from moieties provided in Figure 3
Figure 3
wherein Q is selected from CH or nitrogen;
Rj6 and R17 together forms a phenyl ring optionally substituted with halogen, -0-Ci_3 alkyl, - COOH, -COOd_3 alkyl, -C1-3 alkyl, -OH; or Rj6 and R17 together forms a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen and sulfur; V independently selected from R18, halogen, -C1-4 haloalkyl, -C2-4 alkenyl, -C2 alkynyl, - SR18, -OR18, -SORig, -SO2R18, -N(R18)2, -NHCHO, -NHCOCH3, -C4-6 hetrocycloalkyl, -C4_6 cycloalkenyl, -CO-Ris, -CN and -CHO wherein Rjg at each occurrence is independently selected from hydrogen, -Ci_4 alkyl and -C3_6 cycloalkyl; '{ ' represents the attachment to ring B;
'[' represents the attachment to ring P; ring B is sele
wherein, R19 is selected from hydrogen, halogen, hydroxyl, -N(Ci_6 alkyl)2, -NH(Ci_6 alkyl), - Cj_6 haloalkyl, -C2-6 alkenyl, -C2-6 alkynyl, -Od-6 alkyl, -O d-6 alkenyl, -O d-6 alkynyl,-S- Ci-6 alkyl, -CN, -CO-C1-4 alkyl, -CO— C3-6 cycloalkyl, -COOH, -CONH2, -CON(d-6 alkyl)2, - CONH(d_6 alkyl), -NHC(O) -d-4 alkyl, -N(C1 alkyl)C(O) d-4 alkyl and when R19 is -OC3-6 alkenyl or -O d-6 alkynyl the double and triple bond, respectively, is not on the carbon directly attached to the oxygen atom;
Yi, Y2, Y3 and Y4 are independently and appropriately selected from CH, NR20, O, or S; wherein, R20 is selected from hydrogen, -C1-3 alkyl and -d-6 cycloalkyl;
r is an integer selected from 1 to 3;
'{ ' represents the position of attachment to ring A;
'[' represents the position of attachment to Z;
Z is a bond or is selected from -0-, -S-, -NH-, -N(CM alkyl)-, -N(d-6 alkyl)CO-, -C(0)NH-, -C(d_2 alkyl)2-, -C(O)-, -S(O)-, -S(0)2-, -CH20-, -OCH2-, -CH=CH-, -CH2-, -N(CH2CN)- and -NHC(O)-;
D is a ring containing 5 to 13 membered aryl, fused aryl, heteroaryl, fused heteroaryl, saturated or unsaturated monocyclic, bicyclic or tricyclic carbocyclic ring containing 0-3 heteroatoms selected from nitrogen, oxygen and sulfur; optionally substituted with d_3 alkyl; or D is selected from -d-6 alkyl, -C3-6 cycloalkyl, -C1-4 alkyl-O-d^ alkyl, -C3-6 cyclolkyl-O- d-4 alkyl, -C1-4 alkyl-0-C3_6 cycloalkyl, -d_4 haloalkyl-O-d^ alkyl, -C1-4 haloalkyl-S-d-4 alkyl and -C1-4 alkyl-S-C2-4 alkyl; with the proviso that when:
ring B and D are phenyl;
Z is selected from -0-, -S-, -NH-, -N(d_4 alkyl)- or -C(O)-;
Ring A is selected from
and V is hydrogen then:
R2 is not selected from -C(0)R5, -C(S)R5, -S02R5, -SOR5 wherein R5 is Ry; and
Rj and R2 together does not form the following group
In an embodiment the present invention provides a compound of Formula I; wherein
Rj is hydrogen;
R2 is selected from -CN, -C(0)R5, -C(S)R5, -S02R5 wherein,
R5 is selected from a group comprising Ry, -Ci_6 alkyl, -C3-6 cycloalkyl, -Ci_3 heterocycloalkenylalkyl, NH C6-10 aryl, Cs_io heteroaryl containing 1-3 heteroatoms selected from oxygen, nitrogen or sulfur, wherein
alkyl and cycloalkyl are optionally further substituted with CN group; and aryl, heteroaryl and heterocycloalkenylalkyl are optionally substituted with one or more halogen;
R5' is selected from a group consisting of -C2_6 alkenyl, -C2_6 alkynyl, -CH=CH-(CH2)n- COOR6 and -C(=CH2)CH2COOR6;
n is an integer and at each occurrence is selected from 0 to 3;
R6 is selected from hydrogen, -Ci_3 alkyl; or R2 is a 6 membered heteroaryl ring substituted with at least one group selected from halogen, C3-6 alkenyl, C3-6 alkynyl and -SH group; and optionally further substituted with one or more groups selected from S-C2^ alkenyl, -COOH, -OCi_6 alkyl, -CON(-Ci_3 alkyl), -N02 and -CONH2; or R2 is a group selected from following moieties
wherein, Rs and R9 are independently selected from hydrogen and Ci-6 alkyl, or Rs and R9 together forms 4 to 10 membered mono-cyclic ring optionally substituted with one or more d_3 alkyl group;
EWG is-C(0)Od_3 alkyl;
wherein, Rn and Rj2 are independently selected from hydrogen, Ci_6 alkyl, C2-6 alkenyl, - COOR and -C4_6 heteroaryl containing 1-3 heteroatoms selected from oxygen, nitrogen or sulfur; wherein R is hydrogen or Ci_6 alkyl and the heteroaryl is optionally substituted with one or more halogen atoms; or Rn and Rj2 together forms substituted or unsubstituted 5 to 6 membered saturated or unsaturated mono carbocyclic ring or a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen and nitrogen; and the carbocyclic and the heterocyclic rings are optionally further substituted with one or more halogen atoms;
ring P is 4 to 6 membered saturated monocyclic, ring containing one additional heteroatom selected from nitrogen;
ring A is a heterocycle selected from followin moieties
ring B is phenyl;
Z is selected from -0-, -C(0)NH- and -NHC(O)-;
D is phenyl or pyridine ring unsubstituted or substituted with Ci_3 alkyl.
In another preferred embodiment the present invention provides a compound of Formula I, wherein Rj is hydrogen; R2 is -C(0)Rs or a 6 membered heteroaryl ring substituted with one or more halogen radical;
or R2 is a group selected from following moieties
wherein, Rg and R are independently selected from hydrogen and Ci_6 alkyl, or Rg and R together forms 4 to 10 membered mono-cyclic ring optionally substituted with one or more Ci-3 alkyl group;
EWG is-C(0)Od_3 alkyl;
ring A is a heterocycle selected from following moieties.
The compounds of the present invention can be used as selective agents as inhibitors of BTK either alone or in combination with other related kinases inhibitors, or any other appropriate drugs. Thus the compound of present invention finds use in treating cancer, particularly in haematological malignancies such as chronic lymphocytic leukaemia (CLL), mantle cell lymphoma (MCL), follicular lymphoma (FL) or even disorder such as diffused large B-cell lymphoma (DLBCL) etc., in which the activated B-cell need to be controlled or the proliferation needs to be down regulated. In an embodiment the compounds of present invention can be prepared by any of the following me
following moiety
PG represents an amino protecting group such as a Boc, Fmoc or CBZ which can be cleaved easily under acidic, basic or neutral conditions to the substituted hydrazines of interest. L is leaving group such as halogen or a alkyl-sulfonate.
In method A, cyclic amines represented by (i) are treated with alkali nitrites under acidic condition or by the use of an alkyl nitrite, to obtain the nitroso derivatives such as (ii), which on further reduction results in some of the hydrazines of interest as in (iii).
Hydrazines (iii) can also be prepared by direct displacement of a leaving group (LG) on nitrogen such as mesylate, tosylate, brosylate, triflates or nonaflates to give protected hydrazino derivatives such as (iv) which on deprotection render compounds represented by (iii) as depicted in Method B
In Method C, some of the hydrazines are prepared by treatment of acyclic compounds such as (v) with mono-N-protected hydrazides to obtained directly the cyclic product represented by (iv) which on de -protection under suitable conditions result in required hydrazines (iii). The hydrazines of Formula iii can be converted into compound of Formula I by reacting them
1 2
with LG-R and/or LG-R groups wherein LG is a leaving group.
The required hydrazines on different A-ring moieties can be synthesized as per the general methods outlined in Scheme-2-7 as iven below.
Accordingly, 'A' ring as a pyrazinopyrimidine can be prepared by a sequence starting with condensation of melanonitrile with properly substituted acid chlorides and the condensation product on treatment with hydrazines can give the cyclized amino-pyrazoles as shown. When pyrazolo compound on treatment with formaldehyde and ammonia results in the central A ring of requirement.
In another embodiment 'A' ring as a starting material can be elaborated to the required hydrazines by functional transformations as outlined in Scheme-3.
As shown in Scheme-3 pyrazolo-pyrimidines (Q=N) or pyrazolo-pyridines (Q= CH) can be halogenated at 3-position and then can be treated with nitrogen hetero-cycle of the choice from 4-7 membered ring containing a leaving group 'L' such as halogen or a alkyl-sulfonate at appropriate position to form a C-N bond. Alternatively, 'L' can be a hydroxyl group which participate in Mitsunobu reaction to form C-N bond. Later the halogenated derivative can further be elaborated by treating with a boronate under Suzuki coupling condition to obtain the skeletons of the requirement which can be transformed to the hydrazines of choice.
In another embodiment 'A' ring is an imidazole -pyrazine envisioned can be synthesized as er the brief Scheme-4 outlined hereunder.
Accordingly, a commercially available 2-chloro-3-cyanopyrazole can be reduced either by known hydrogenation method or by involving reducing agents such as lithium aluminium hydride to convert into an amine which can be treated with an acid chloride or appropriately activated acid to form an amide bond as shown in Scheme-4. The amide intermediate can be ring closed to obtain the 'A' ring as an imidazole-pyrazine scaffold of choice. Which can be further elaborated to the hydrazines by the known methods known in the art.
In a fourth embodiment as outlined in Scheme-5, 'A' ring can be modified as pyrazolo- pyrimidine or pyrazolo-pyridine scaffolds which are differently disposed from the earlier described embodiments.
Commercially available chlorofluropyrimidine or chlorofluropyridine can be acylated with suitable acid chloride moiety and then treated with hydrazine to form the scaffold of interest. Further elaboration can result in some of the hydrazines of current interest.
In yet another embodiment the 'A' ring scaffold can be modified to a 2-imidazalone fused
Bz in Scheme-6 is a benzyl group which may be substituted of unsubstituted. From the known dichloronitropyrimidine, the A-ring can be synthesized by successive substitutions with different amines of choice followed by the reduction of the nitro group to obtain a 1,2- diamino function which can be ring closed to form the urea linkage by either CDI or 4- nitrophenyl chloroformate. These intermediates can be further elaborated by a Suzuki or by Buchwald coupling methods to obtain the needed disposition of groups and the hydrazines of choice. The linkage of the groups is not confined to one of the fused heterocyclic rings such as pyrazole or imidazole but can be viewed as a substituents attached in both the rings as given
in some of the scaffolds of Figure-3. Scheme-7 given below outlines the synthesis of such hyrdrazines.
Accrodingly, substituted dichloropurine can be functionalized to obtain the required hydrazine in few steps. Hydrazines prepared as per the above methods described in Scheme- 1 to Scheme-7, can be treated with suitable electrophilic compounds to afford the compounds of Formula I. Some of the representative electrophilic compounds are provided in Figure-5. The electrophilic compound may require some functional modifications depending upon the type .
Figure-5
Ar in Figure-5 is an aryl group and Het.Ar is a heteroaryl group. Ra and R are independently H, alkyl Ci_6, cycloalkyl Cj,.(,-
In another embodiment the hydrazines prepared as per the process provided in Scheme- 1 to Scheme-7 were treated with aromatic electrophiles. Some of the representative aromatic electrophilic compounds are provided in Figure-6. The aromatic electrophilic compound may also require some functional modifications depending upon the type of the substitution on them and such modifications are under the purview of a person skilled in the art.
Figure-6
In yet another embodiment, the hydrazines prepared according to the methods described above can be treated with substrates containing aldehydes and ketones containing either saturated or unsaturated functionalities as condensing agents to obtain the compound of Formula I. Representative aldehydes and ketones are provided in Figure-7.
O'
OR
OHO^ / CHO. , (COOR
λ Δ X CHO^ CH0"CN CHO\/COOR
COOR
Figure-7
Similarly hydrazines prepared according to the methods described above can be treated with 1,3 diketones, 1,3 ketoesters or 1,2 ketoesters to afford compound of Formula I having ene-
amide, ene-ester etc. linked substitution. The representative 1,3 diketones, 1,3 ketoesters or 1,2 ketoesters are provided in Figure-8
Representative compounds of Formula I are presented in Table 1.
Table 1
Examples:
A typical synthesis of the product 1.1 presented in Table- 1 was accomplished from already known intermediates such as l-aminopiperidin-4-ol and 3-(4-phenoxyphenyl)-lH- pyrazolo[3,4-(f]pyrimidin-4-amine as described in Miscoria et al. [US 2006/0064065] and Hirst et al. [US2002/0156081] respectively. Also intermediate l-nitrosopiperidin-4-ol was prepared as per the procedures described in Bush et al. [WO2007/127688A2] which on usual methanesulphonation results in mesylated intermediate (l-nitroso-4-piperidyl) methanesulfonate required for current work.
Other compounds of interest such as 1.6, 1.11, 1.14 and 1.17 were also accomplished from the intermediate l-(l-amino-4-piperidyl)-3-(4-phenoxyphenyl)pyrazolo[3,4-(i]pyrimidin-4-amine as per the procedures described here under and not limited to these compounds only. Example 1: fert-Butyl N-(4-hvdroxy-l-piperidyl)carbamate
To a stirred solution of l-aminopiperidin-4-ol (9.6 g) in THF (100 mL) in around bottom (RB) flask, triethylamine (16.4 mL) and (Boc)20 (21.4 mL) were added and stirred for 3 h. The content of the flask was concentrate under reduced pressure. Water was added to the residue and the mixture was extracted with ethyl acetate. The organic layer was dried over anhydrous sodium sulfate and concentrated. The residue was subjected to column chromatography to afford the title compound as an off-white solid.
Example 2: ri-(tgrt-Butoxycarbonylamino)-4-piperidvnmethanesulfonate
To a cooled (0-5 °C) stirred solution of tert-butyl N-(4-hydroxy-l-piperidyl)carbamate boc- hydrazide (1.7 g) in dichloromethane (34 ml) under nitrogen, triethylamine (1.3 ml) and methanesulfonyl chloride (0.73 ml) were added, and the mixture was stirred at ambient temperature for 1 h. The reaction mixture was quenched into cold water and the organic layer separated. The organic phase was dried over anh. sodium sulfate, filtered and concentrated to obtain title compound as a pale yellow oily mass, which was used as such in the next step without further purification.
Example 3: fert-Butyl jV-r4-r4-amino-3-(4-phenoxyphenyl)pyrazolor3,4-<flpyrimidin-l- yll - 1 -piperid yllcarbamate
To a stirred solution of the intermediate 3-(4-phenoxyphenyl)-lH-pyrazolo[3,4-(f]pyrimidin- 4-amine (2.38 g) in DMF (20 ml), potassium carbonate (2.15 g) and a solution of [l-(tert- butoxycarbonylamino)-4-piperidyl]methanesulfonate (2.30 g) in DMF (5 ml) were added. The reaction mixture was heated at 80 °C for 16 h and DMF was distilled off under reduced pressure. The crude mass was dissolved in dichloromethane and purified by column chromatography, to obtain the title compound as an off-white solid.
Example 4: l-(l-Aminopiperidin-4-yl)-3-(4-phenoxyphenyl)-iH-pyrazolor3,4- fflpyrimidin-4-ylamine
A stirred solution of teri-butyl N-[4-[4-amino-3-(4-phenoxyphenyl)pyrazolo[3,4-(f]pyrimidin- l-yl]-l-piperidyl]carbamate (1.7 g) in 1,4-dioxane (20 ml) was treated with concentrated hydrochloric acid (cone. HQ) (4.0 ml) at ambient condition for 16 h and solution was concentrated, basified with triethylamine and extracted with dichloromethane. The organic layer was washed with water, dried over anh. sodium sulfate and evaporated under reduced pressure to obtain the title compound as an off white solid.
Example 5: l-Nitrosopiperidin-4-ol
To a cold (0-10 °C) and stirred solution of 4-hydroxypiperidine (50 g, 0.49 mole) in a mixture of 300 ml water and acetic acid (45 g, 0.741 mole), sodium nitrite (68.2 g, 0.99 mole) was added and the mixture was stirred for 1-2 h at ambient temperature. The reaction mixture was quenched with sodium carbonate extracted with ethyl acetate and the organic layer was concentrated to obtain title compound as off white solid.
Example 6: (l-Nitroso-4-piperidyl) methanesulfonate
To a cold (0-5 °C) solution of l-nitrosopiperdin-4-ol (33 g, 0.25 mol) in dichloromethane (300 ml) triethylamine (88 ml, 0.6337 mol) was added. Methanesulfonyl chloride (30 ml, 0.38 mol) was added drop-wise into the mixture. The reaction mixture was stirred for 1 h at ambient temperature. The reaction mixture was quenched into water and separated the organic phase was concentrated to obtain the title compound as an off white solid.
Scheme-8
Example 7: 4-Bromo-N-(4-methyl-2-pyridyl)benzamide (8-5)
To a stirred solution of 4-bromobenzoic acid (10 g) in toluene (40 ml), thionyl chloride (7.2 mL) was added drop-wise at 25 °C. The reaction mass was heated to reflux for 3 h and concentrated to remove the volatiles under reduced pressure and the residue was dissolved in dicholormethane (50 mL). A solution of 2-amino-4-methylpyridine (8-4; 6 g) in a mixture of dichloromethane (30 mL) and pyridine (8 mL) previously cooled in ice-bath under nitrogen was drop-wise added into the reaction mass. The reaction mass was stirred at ambient condition for 6 h. The reaction mass was quenched with water and the organic phase was separated, dried (over anh. sodium sulfate), filtered, concentrated and purified by column chromatography to obtain title compound as a white solid.
Example 8: jV-(4-Methyl-pyridin-2-yl)-4-(4,4,5,5-tetramethviri,3,21dioxaborolan-2-yl)- benzamide (8-6)
To a stirred solution of 4-bromo-N-(4-methyl-2-pyridyl)-benzamide (8-5; 3.0 g) in 1 ,4- dioxane (10 mL), Z?i5,(pinacoloto)diboron (3.15 g), potassium acetate (3 g) and [Ι, - Z?i5'(diphenylphosphino)ferrocene]-dichloropalladium(II) complex (0.24 g) were added. The contents of the flask were refluxed for 3 to 4 h, cooled and filtered over Hyflo®. The filtrate was concentrated and purified by column chromatography to obtain the title compound (2.8 g) as a pale yellow solid. Example 9: 3-Bromo-lH-pyrazolor3,4-^pyrimidin-4-ylamine (8-2)
To a stirred suspension of lH-pyrazolo-[3,4-(i]pyrimidin-4-ylamine (8-1 ; 50 g) in DMF (500 mL) N-bromosuccinimide (66 g) was added and the reaction mass was heated for 5 h at 70 °C. The solvent was removed under reduced pressure. Water was added to the residue and stirred for 30 minutes. The reaction mass was filtered and the solid and washed with diethyl ether. The product was dried under reduced pressure overnight to obtain the title compound as a brown solid.
Example 10: 3-Bromo-l-(l-nitroso-piperidin-4-yl)-lH-pyrazolor3,4-</lpyrimidin-4- ylamine (8-3)
To a stirred suspension of 3-bromo-iH-pyrazolo[3,4-(f]pyrimidine-4-ylamine (8-2; 11 g ) in DMF (150 mL) methanesulfonic acid l-nitrosopiperidin-4-yl ester (17.1 g) and potassium carbonate (18 g) were added. The reaction mixture was heated under nitrogen atmosphere for 12 h at 85 °C. After completion of reaction, the reaction mass was filtered and concentrated under reduced pressure to obtain red oil, which was purified by column chromatography to obtain the title compound as light brown solid.
Example 11: 4-r4-Amino-l-(l-nitroso-piperidin-4-yl)-lH-pyrazolor3,4-<flpyrimidin-3- vn-N-(4-methyl-pyridin-2-yl)benzamide (8-7)
To a stirred solution of 3-bromo-l-(l-nitroso-piperidin-4-yl)-iH-pyrazolo[3,4-(i]pyrimidin- 4-ylamine (8-3; 2.16 g) and N-(5-methyl-pyrimidin-2-yl)-4-(4,4,5,5-tetramethyl- [i,3,2]dioxaborolan-2-yl)benzamide (8-6) (2.8 g) in 1 ,4-dioxane (20 mL) aq. potassium carbonate (2Ν, 5.66 g) and [l, r-B 5'(diphenylphosphino)ferrocene]dichloropalladium(II) complex (0.3 g) were added. The reaction mass was refluxed for 4 h, cooled and filtered over
Hyflo . The filtrate was concentrated and purified by column chromatography to obtain compound title compound as pale yellow solid.
Example 12: 4-r4-Amino-l-(l-amino-piperidin-4-yl)-lH-pyrazolor3,4-^pyrimidin-3-yl1- N-(4-methyl-pyridin-2-yl)benzamide (8-8)
To a stirred and cooled (0-5 °C) suspension of 4-[4-amino-l-(l-nitrosopiperidin-4-yl)-lH- pyrazolo[3,4-ii]pyrimidin-3-yl]-N-(4-methyl-pyridin-2-yl)benzamide (8-7; 1.5 g) in acetic acid (10.5 mL) and water (4.5 mL) was added zinc dust (0.85 g) in small portions. The reaction mass was stirred for one hour at 25 °C and excess zinc was filtered off, basified with aq. sodium hydroxide and extracted with ethyl acetate. The combined organic phase was concentrated to obtain the title compound as a brown solid.
On similar lines several other A ring scaffold containing compounds were prepared by the methods published or known in the art and converted to compounds of Formula I.
Example 13: Cyclopropanecarboxylic acid {4-r4-amino-3-(4-phenoxy-phenyl)- Pyrazolor3,4-<flpyrimidin-l-vn-piperidin-l-yl)amide 1.1:
To a stirred solution of l-(l-aminopiperidin-4-yl)-3-(4-phenoxyphenyl)-iH-pyrazolo[3,4- (f]pyrimidin-4-ylamine (0.5 g) in dichloromethane (10 mL) triethylamine (0.5 mL) was added and the reaction mass was cooled to 0-5 C and treated with neat cyclopropane carbonyl chloride (0.11 mL) for 30 minutes. The reaction mass was quenched into cold water, the organic phase was separated and dried over anh. sodium sulfate. The crude mass was subjected to column chromatography on silica gel (elution with hexane: acetone) to obtain compound 1.1 as an off-white solid.
Example 14: 3-r4-r4-Amino-3-(4-phenoxy-phenyl)pyrazolor3,4-<flpyrimidin-l- yllpiperidin- 1 - ylaminol -c vclopent-2-enone 1.6 :
To a stirred suspension of l-(l-aminopiperidin-4-yl)-3-(4-phenoxyphenyl)-iH-pyrazolo[3,4- (f]pyrimidin-4-ylamine (0.3 g) and molecular sieves in dichloroethane (12 mL) under nitrogen atmosphere, was added 1,3-cyclopentadione (0.22 g) and the mixture was refluxed overnight. The reaction mass was cooled, methanol was added, filtered and evaporated to dryness under reduced pressure. The residue was subjected to column chromatography on silica gel to obtain 1.6 as an off-white solid.
Example 15: l-Cyano-cyclopropanecarboxylic acid {4-r4-amino-3-(4-phenoxy-phenyl)- Pyrazolor3,4-<flpyrimidin-l-vnpiperidine-l-yl)-amide 1.11:
To a stirred solution of l-(l-aminopiperidin-4-yl)-3-(4-phenoxyphenyl)-lH-pyrazolo[3,4- (f]pyrimidin-4-ylamine (0.3 g) in DMF (10 mL) diisopropylethyl amine (0.26 mL), 1-cyano- cyclopropane carboxylic acid (O. lg) were added and treated with HATU (0.34 g) for 120 minutes at ambient condition. The solvent was removed under reduced pressure. Dichloromethane was added to the residue and washed with water. The organic phase was separated, dried (over anh. sodium sulfate), concentrated and subjected to column chromatography to obtain 1.11 as an off-white solid.
Example 16: l-r-(2-Chloro-5-fluoropyrimidin-4-ylamino)piperdin-4-vn-3-(4- phenoxyphenyl)-iH-pyrazolor3,4-<flpyrimidin-4-ylamine 1.14:
To a stirred and cold (0-5 °C) solution of l-(l-aminopiperidin-4-yl)-3-(4-phenoxyphenyl)- 7H-pyrazolo[3,4-i/]pyrimidin-4-ylamine (2 g, 0.005 mol) in THF (40 mL) DIPEA (1.4 mL, 0.0075 mol) was added. Into the reaction mass 2,4-dichloro-5-fluoropyrimidine (1 g) was added portion-wise. The reaction mass was stirred at 25 °C for 1 h. The reaction mass was concentrated under reduced pressure and subjected to column chromatography (eluted with dichloromethane / methanol as mobile phase) to obtain 1. 14 as a white solid. Example 17: l-ri-rr(JE)-But-2-en-(Z)-ylidene1amino1-piperidin-4-vn-3-(4-phenoxy- phenvn-lH-pyrazolor3,4-</lpyrimidin-4-ylamine 1.17:
To a stirred suspension of l-(l-aminopiperidin-4-yl)-3-(4-phenoxyphenyl)-iH-pyrazolo[3,4- (f]pyrimidin-4-ylamine (0.5 g) and molecular sieves in dichloroethane (20 mL), crotonaldehyde (0.26 g) was added under nitrogen atmosphere and the reaction mass was heated at reflux for about 5-6 h. The reaction mass was cooled and methanol was added. The mixture was filtered and evaporated to dryness under reduced pressure. The crude was subjected to column chromatography on silica gel to obtain 1.17 as a yellow solid.
Example 18: 4-ri-(l-Acryloylamino-piperidine-4-yl)-4-amino-iH-pyrazolo-r3,4- <flpyrimidine-3-vn-jV-(5-methyl-pyridin-2-yl)-benzamide 1.33
To a cold (-55 °C to -65 °C) and stirred solution of 4-[4-amino-l-(l-amino-piperidin-4-yl)- iH-pyrazolo[3,4-i/]pyrimidin-3-yl]-N-(5-methyl-pyridin-2-yl)benzamide (8-8) (0.5 g) in dichloromethane (10 mL) was added triethylamine (0.23 mL). A solution of acryloyl chloride (0.1 mL) in dichloromethane (5mL) was then added drop-wise into it and temperature of
reaction mass was raised to 25 °C. The reaction mass was quenched with water and organic layer was separated dried and subjected to column chromatography to obtain 1.33 as a white solid.
SCHEME 9
Examples 19: N, N-Dibenzyl-6-chloro-5-nitro-pyrimidin-4-amine (9.2)
A solution of dibenzylamine (26.17g) in dichloromethane (60 mL) was added drop-wise into a solution of 4,6-dichloro-5-nitropyrimidine (20.0 g) in dichloromethane (140 mL) over an ice bath. Triethylamine (18.3 mL) was added, and the mixture was stirred for 1 hour. Water was added to the reaction mixture, the organic layer was washed with a saturated aqueous sodium chloride solution and dried over anhydrous sodium sulfate. The solvent was removed under reduced pressure to obtain the title compound (32.1 g) as a pale yellow solid.
Example 20: fert-Butyl 4-rr6-(dibenzylamino)-5-nitro-pyrimidin-4-vnamino1piperidine- 1-carboxylate (9.3)
N,N-Dibenzyl-6-chloro-5-nitro-pyrimidin-4-amine (18g) and teri-butyl 4-aminopiperidine-l- carboxylate (15.15 g) were dissolved in dioxane (90.0 mL). Triethylamine (10.5 mL) was added and the mixture was stirred for 16 hours at 60 °C. The reaction mixture was cooled to room temperature and the solvent was distilled off. Residue was extracted with ethyl acetate and the organic layer was washed with water, saturated aqueous sodium chloride solution and dried over anhydrous sodium sulfate. The solvent was distilled off after filtration and the residue was subjected to silica gel column chromatography to obtain the title compound (22.0 g) as an off-white solid.
Example 21: fert-Butyl 4-rr5-amino-6-(dibenzylamino)pyrimidin-4-vnamino1piperidine- 1-carboxylate (9.4)
An ethyl acetate (400 mL) solution of compound (9.3) (20.0 g) was added drop-wise into a suspension of zinc (26.2 g) and 3.0 M aqueous ammonium solution (12.3 g) over an ice bath. The temperature was immediately raised to room temperature. After stirring for 16 hour, the reaction mixture was filtered through Celite™ and the solvent was distilled off. The residue was subjected to silica gel column chromatography to obtain the title compound (12.0 g) as an off-white solid. Example 22: fert-Butyl 4-r6-(dibenzylamino)-8-oxo-7H-purin-9-yl1piperidine-l- carboxylate (9.5)
Compound 9.4 (12.0 g) and Ι,Γ-carbonyl diimidazole (7.96 g) were dissolved in tetrahydrofuran (240 mL) and the solution was stirred for 16 hours at 65 C. The solvent was distilled off from the reaction mixture, water was added and extracted with ethyl acetate. The organic layer was washed with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulfate, filtered and the solvent was distilled off. The residue was subjected to silica gel column chromatography to obtain the title compound (11.0 g) as an off-white solid. Example 23: fert-Butyl 4-(6-amino-8-oxo-7H-purin-9-yl)piperidine-l-carboxylate (9.6)
The compound 9.7 (9.0 g) was dissolved in acetic acid (90 mL), 5 % wet Pd on Carbon (10.0 g) was added, hydrogen gas replacement was carried out and starring was performed for 16 hours at 60 C. The reaction mixture was filtered through Celite™. The solvent was distilled off from the reaction mixture, water was added, and the extraction with ethyl acetate was performed. The organic layer was washed with saturated aqueous sodium chloride solution then dried over anhydrous sodium sulfate, and the solvent was distilled off. The residue was purified by silica gel column chromatography to obtain the title compound (4.2 g) as an off- white solid. Example 24: fert-Butyl 4-r6-amino-8-oxo-7-(4-phenoxyphenyl)purin-9-yl1piperidine-l- carboxylate (9.7)
At room temperature, p-phenoxy phenyl boronic acid (7.63 g), copper(II) acetate (4.73 g), molecular sieve 4A (8.0 g), pyridine (4.8 mL) and triethylamine (8.2 mL) were added to a suspension of compound 9.6 (4.0 g) in dichloromethane (200 mL) and stirred for 72 hours.
The reaction mixture was filtered through Celite and the residue was purified by silica gel column chromatography to obtain the title compound (4.0 g) as an off-white solid.
Example 25: 6-Amino-7-(4-phenoxyphenyl)-9-(4-piperidyl)purin-8-one (9.8)
At room temperature, 4 N HCl in dioxane (44 mL) was added to a suspension of compound 9.7 (4.0 g) in methanol (40 mL) and the mixture was stirred for 4.0 hours. The solvent was then distilled off to obtain the title compound (2.0 g) as an off-white solid.
Example 26: 6-Amino-9-(l-nitroso-4-piperidyl)-7-(4-phenoxyphenyl)purin-8-one (9.9) A solution of sodium nitrite (0.42 g) in water (10 mL) was added drop-wise into a solution of compound 9.8 (1.0 g) in acetic acid (15.0 mL) at low temperature (-5 to 0 C) and stirred at room temperature for 6 hours. The solvent was distilled off from the reaction mixture, water was added, and the extraction with ethyl acetate was performed. The organic layer was washed with saturated aqueous sodium chloride solution then dried over anhydrous sodium sulfate, and the solvent was distilled off. The residue was purified by silica gel column chromatography to obtain the title compound (0.8 g) as an off-white solid.
Example 27: 6-Amino-9-(l-amino-4-piperidyl)-7-(4-phenoxyphenyl)purin-8-one (9.10)
An Ethyl acetate (30 mL) solution compound 9.9 (0.80 g) was dripped into a mixture of zinc (1.2 g) and 3.0 M aqueous ammonium solution (0.57 g) on an ice bath, , followed by stirring at room temperature for 6 hours. The reaction mixture was filtered through Celite™. The organic layer was washed with saturated aqueous sodium chloride solution then dried over anhydrous sodium sulfate, and the solvent was distilled off. The residue was purified by silica gel column chromatography to obtain the title compound (0.5 g) as an off-white solid
Example 28 : N- Γ4- Γ6- Amino-8-oxo-7-(4-phenoxyphenyl)purin-9- yll - 1 -piperidyll -3- (2,5- dioxopyrrol-l-yl)propanamide (1.39)
To stirred solution of compound 9.10 (0.2 g) in MDC (10.0 mL) and triethylamine (0.13 mL), solid 3-(2,5-dioxopyrrol-l-yl) propanoic acid (0.16 g) and l-ethyl-3-(3- dimethylaminopropyl) carbodiimide hydrochloride (0.15 g) was added ,followed by stirring for 16 hours . The reaction mass was quench with water, the organic phase was separated, dried over anhydrous sodium sulphate and filtered. Evaporated the filtrate and the crude mass was subjected to column chromatography to obtain the title compound (34 mg) as an off-white solid.
Example 29: 2,4-Dichloro-5-iodo-7H-pyrrolor2,3-<f1pyrimidine (10.2)
To a stirred solution of 2,4-dichloro-7H-pyrrrolo[2,3-(f]pyrimidine (25 g) in DMF (100 mL) was charged N-Iodosuccinamide (31.41g). The reaction mixture was stirred for 3-4 h at ambient temperature and poured into water. Precipitates were filtered and washed with water (500 mL). Filtered solid was suck dried and extracted into ethylacetate (800 mL) and washed with water (300 mL). Organic layer was dried over anhydrous sodium sulphate and concentrated under vacuum. Trituration of the solid with hexanes (125 mL) followed by filtration and drying afforded an off-white solid (30 g).
Example 30: tert-Butyl 4-(2,4-dichloro-5-iodo-pyrrolor2,3-^lPyrimidin-7-yl)piperidine- 1-carboxylate (10.3)
To a stirred solution of compound 10.2 (10 g) in THF (200 mL) was charged, triphenylphosphine (16.68 g) and maintained at ambient temperature. The reaction flask was flushed with nitrogen and charged DEAD (8.28 mL) and maintained at ambient temperature for 16 h. The reaction mass subjected to silica gel column chromatography to obtain the title compound (12 g) as an off-white solid,
Example 31: fert-Butyl 4-(4-amino-2-chloro-5-iodo-pyrrolor2,3-<flpyrimidin-7- vDpiperidine- 1-carboxylate (10.4)
To a stirred solution of compound 10.3 (12 g) in 1 ,4-dioxan (120 mL) was added aq. ammonia solution (25 %, 60 mL). The contents were transferred into an autoclave and heated at 80-85 °C for 16 h. Concentrated the reaction mass, suspended the residue in water (25 mL), filtered and dried to obtain the title compound (10 g) as an off-white solid.
Example 32: fert-Butyl 4-r4-amino-2-chloro-5-(4-phenoxyphenyl)pyrrolor2,3- ^Pyrimidin-7-yllpiperidine-l-carboxylate (10.5)
To a stirred solution of compound 10.4 (11 g) in 1,4-dioxan (155 mL) were charged 4- phenoxyphenylboronicacid (6.4 g) and aq. sodium carbonate (7.3 g in 30 mL). The reaction mass was flushed with nitrogen and Z?i5,-(triphenylphosphine)palladium (II) dichloride (1.61 g). The contents were stirred at 80-85 °C for 2 h and concentrated. The residues were partitioned between ethyl acetate and water. Organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure after filtration. The residues were purified by column chromatography to afford the title compound (8.5 g) an off-white solid.
Example 33: 2-Chloro-5-(4-phenoxyphenyl)-7-(4-piperidyl)pyrrolor2,3-<flpyrimidin-4- amine (10.6)
To a cooled (0 °C) and stirred solution of compound 10.5 ( 8 g) in dichloromethane (80 mL) was added trifluoroaceticacid (40 mL).The reaction mass was allowed to warm to ambient temperature and stirred for 4-5 h. The reaction mixture was concentrated and residues were partitioned between dichloromethane (100 mL) and 5 % aq. sodium carbonate solution. Removal of solvents afforded the title compound (5 g) as an off-white solid. Example 34: 2-chloro-7-(l-nitroso-4-piperidyl)-5-(4-phenoxyphenyl)pyrrolor2,3- fflpyrimidin-4-amine (10.7)
To a stirred solution of compound 10.6 (5 g) in THF (200 mL) was added isoamylnitrite (1.33 g) and refluxed for 24 h. At the end the reaction mixture was concentrated and residues were subjected to column chromatography to give the title compound (4.5 g) as a golden yellow solid.
Example 35: 7-(l-amino-4-piperidyl)-2-chloro-5-(4-phenoxyphenyl)pyrrolor2,3- <flpyrimidin-4-amine (10.8)
To a solution of compound 10.7 (4.5 g) in glacial acetic acid (31 mL) was added water (13.5 mL) and cooled to 0-5 °C in ice-salt bath. Activated zinc (2.61 g) was added during period of 10-20 minutes and allowed to warm to room temperature. The contents were filtered over Celite™ and mother liquor was poured into excess aq. 20 % sodium carbonate solution and extracted with mixture of ethyl acetate: THF. Organics were dried over anhydrous sodium sulphate and concentrated to yield the title compound (2.5 g) as a slight pinkish solid.
Example 36: ^V-[4-f4-amino-2-chloro-5-(4-phenoxyphenyl)pyrrolo[2,3-</|pyrimidin-7-vi|- l -piperidyijprop-2-enamitle (L40)
To a cooied (-10 UC ) and stirred solution of compound 10.8 (0,8 g) in dichloromethane ( 10 ml.) was added iriethylamine (0,38 rnL) and flushed with nitrogen , Acryloyl chloride (0.2 mL) was added during 4-5 minutes and maintained for 30 minutes. The reaction was quenched with water ( 10 mL) and layers were separated. Orgamcs layer was dried and concentrated and subjected to chromatographic separation with mixture of ethyl acetate and hexanes to afford the title compound (0.1 g) as an off-white solid. Biological Data:
Radioligand binding assay:
BTK (h) was incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA. 250 μΜ VEKJGEGTYG V V YK (Cdc2 peptide), 10 mM MgAcetate and [γ-33Ρ-ΑΤΡ] (specific activity approx. 500 cprn/pmol, concentration as required). The reaction was initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction was stopped by the addition of 3 % phosphoric acid solution. 1 0 μΐ. of the reaction was then spotted onto a P30 filtermat and washed three times for 5 minutes with 75 mM phosphoric acid and once with methanol prior to drying and scintillation counting.
The compounds of present invention showed more than 50 % inhibition of BTK at 100 nM. Table 2 provides percentage inhibition at 10 nM and 100 nM for few representative compounds in radioligand binding assay.
Table 2
Claims
1. A compound of Formula I and pharmaceutically acceptable salts thereof
wherein, Rj is selected from a group consisting of hydrogen, -C1-4 alkyl, -C3 alkenyl, -C3 alkynyl, -C1-4 haloalkyl, -C2-4 haloalkenyl, -C3 haloalkylalkynyl, -C1-4 cyanoalkyl, -C2-4 cyanoalkenyl, -C2-4 cyanoalkynyl, -C2-5 O-alkyl, -C2-5 O-haloalkyl, -C3-6 cycloalkyl, -C4-6 heterocycloalkyl containing 1-3 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C1-4 alkyl or -CO-Ci_ 4 alkyl group; and when Rj is alkenyl or alkynyl, the double and triple bond, respectively, is not on the carbon directly attached to the nitrogen atom;
R2 is selected from hydrogen, -C1-4 alkyl, -C3-4 alkenyl, -C3-4 alkynyl, -C1-4 haloalkyl, -C2-4 haloalkenyl, -C3_4 haloalkylalkynyl, -C1-4 cyanoalkyl, -C2-4 cyanoalkenyl, -C2 cyanoalkynyl, - C2-5 O-alkyl, -C2-5 O-haloalkyl, -C3-6 cycloalkyl, -CN, -C(0)R5, -C(S)R5, -S02R5, -SOR5, - CONHSO2R5, -CSNHSO2R5, -CONHCOR5, -CSNHCOR5, -CON(d_4 alkyl)COR5, , - C(S)N(Ci-4 alkyl)COR5, -CON(d_4 alkyl)S02R5, -CSN(C1 alkyl)S02R5 -C(=NR6)R5, - C(=NR6)N(R6)C(0)R5, -C4-6 heterocycloalkyl containing 1-3 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C1-4 alkyl and -CO-Cj_4 alkyl group, and when R2 is alkenyl or alkynyl, the double and triple bond, respectively, is not on the carbon directly attached to the nitrogen atom; wherein R5 is selected from a group comprising Ry, hydrogen, -NH2, -NHOH, -NRi(Ci_4 alkyl)-, -Ci_6 alkyl, -C3_6 cycloalkyl, -Ci_6 haloalkyl, -C3_6 cycloalkenyl, -Ci_3 heterocycloalkenylalkyl, -C3_6 heterocycloalkyl, -NH C6-10 aryl, Cs_io heteroaryl containing 1- 3 heteroatoms selected from oxygen, nitrogen or sulfur; wherein alkyl, cycloalkenyl, cycloalkyl are optionally further substituted with CN group; and aryl, heteroaryl and heterocycloalkenylalkyl are optionally substituted with one or more halogen; and Rs> is selected from a group consisting of -C2-6 alkenyl, -C2-6 alkynyl, -C3_6 alkenylaryl, -C3_6
alkenylheteroaryl, -C3_6 alkynylaryl, -C3_6 alkynylheteroaryl, -CH=CH-(CH2)n-COOR6, - C(=CH2)CH2COOR6, -C≡C-(CH2)n-COOR6, -CH=CH-(CH2)n-CN, -C≡C-(CH2)n-CN, - CH=CH-(CH2)n-OR6, -C≡C-(CH2)n-OR6, -CH=CH-(CH2)n-NR6R7 and -C≡C-(CH2)n-NR6R7; n is an integer and at each occurrence is selected from 0 to 3; m is an integer and at each occurrence is selected from 1 to 3; R6 and R7 at each occurrence are independently selected from hydrogen, -Ci_3 alkyl and -C4_6 cycloalkyl or R6 and R7 together forms a 4-6 membered ring optionally containing one additional heteroatom selected from oxygen, nitrogen, or sulfur; or R2 is an aryl group substituted with at least one halogen atom and at least one group at ortho or para position with respect to halogen atom selected from -CN, -COOH, -COO-Ci_3 alkyl, -CON(-d_3 alkyl), -N02, -S02d_3 alkyl, -S02NH2, -S02NH-d_3 alkyl, -S02N(d_3 alkyl)2, -CO-d_3 alkyl, -CONH2, -CONH(d_3 alkyl), -CON(d_3 alkyl)2; or R2 is a 6 membered heteroaryl ring containing 1-3 nitrogen atoms or 5 membered ring containing 1 to 3 heteroatoms selected from oxygen, nitrogen, or sulfur wherein the 6 membered or 5 membered ring is substituted with at least one group selected from halogen, C3_6 alkenyl, C3_6 alkynyl, -COOH and -SH group; and optionally further substituted with one or more groups selected from -CN, S-C2_4 alkenyl, -COOH, -OCi_6 alkyl, -COO-Ci_3 alkyl, - CON(-d-3 alkyl), -N02, -S02d-3 alkyl, -S02NH2, -S02NH-d-3 alkyl, -S02N(d-3 alkyl)2, - CO-d_3 alkyl, -CONH2, -CONH(d_3 alkyl), -CON(d_3 alkyl)2;
wherein, Rg and R are independently selected from hydrogen, Ci_6 alkyl, C2_6 alkenyl, C3_7 cycloalkyl or R8 and R9 together forms 4 to 10 membered mono or bicyclic carbocyclic ring optionally substituted with one or more Ci_3 alkyl group or a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen, or sulfur and when the
heteroatom is nitrogen it is optionally further substituted with -C1-4 alkyl or -CO-Ci_4 alkyl group;
EWG is an electron withdrawing group selected from -COOR10, -CON(Rio)2, -CN, -N02, - SO3H, -S02N(Rio)2, -SO2R10 wherein Rjo is selected from hydrogen, -Ci_3 alkyl and -C4_6 cycloalkyl;
T is a heteroatom selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C1-4 alkyl or -CO-C1-4 alkyl group; p is an integer selected from 1 and 2;
' { ' is the point of attachment of R2 with nitrogen atom;
or
Figure 2 wherein, Rn and R12 are independently selected from hydrogen, Ci_6 alkyl, C2-6 alkenyl,
cycloalkyl, -COOR, -CON(R)2, -CH=CHCOOR, -CH=CHCONRR', -C4-6 heteroaryl containing 1-3 heteroatoms selected from oxygen, nitrogen or sulfur, the heteroaryl is optionally substituted with one or more halogen atoms; wherein R and R' are selected from hydrogen or Ci_6 alkyl or R and R' together with the nitrogen to which they are attached forms a 5-6 membered saturated or unsaturated ring; or Rn and Rj2 together forms 4 to 10 membered mono or bicyclic saturated or unsaturated carbocyclic ring or a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen and sulfur and when the heteroatom is nitrogen it is optionally further substituted with -C1-4 alkyl or
alkyl group; the carbocyclic and the heterocyclic rings are optionally further substituted with one or more halogen atoms;
Rj3 and R14 are independently selected from hydrogen, Ci_6 alkyl, C2-6 alkenyl,
cycloalkyl or R13 and R1 together forms 4 to 10 membered mono or bicyclic carbocyclic ring or a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen, or sulfur and when the heteroatom is nitrogen it is optionally further substituted with -Ci_4 alkyl or -CO-C1 alkyl group;
EWG2 is an electron withdrawing group selected from -COOR15, -CON(Rj5)2, -CN, -NO2, - SO3H, -S02N(Rj5)2, -SO2R15 wherein RJS at each occurrence is independently selected from hydrogen, -C1-3 alkyl and -C4-6 cycloalkyl; q is an integer selected from 1 and 2;
' { ' is the point of attachment of Rj and R2 with nitrogen atom; R3 and R4 are independently selected from hydrogen, halogen, Ci-6 alkyl and C3-6 cycloalkyl; ring P is 4 to 13 membered saturated or unsaturated monocyclic, bicyclic or tricyclic ring optionally containing one additional heteroatom selected from oxygen, nitrogen and sulfur; ring A is a heterocycle selected from moieties provided in Figure 3
Figure 3
wherein Q is selected from CH or nitrogen;
Rj6 and R17 together forms a phenyl ring optionally substituted with halogen, -0-Ci_3 alkyl, - COOH, -COOd_3 alkyl, -d_3 alkyl, -OH; or Rj6 and R17 together forms a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen, nitrogen and sulfur;
V independently selected from R18, halogen, -C1-4 haloalkyl, -C2-4 alkenyl, -C2_4 alkynyl, - SR18, -OR18, -SORig, -SO2R18, -N(R18)2, -NHCHO, -NHCOCH3, -C4-6 hetrocycloalkyl, -C4_6 cycloalkenyl, -CO-Ris, -CN and -CHO wherein Ris at each occurrence is independently selected from hydrogen, -Ci_4 alkyl and -C3_6 cycloalkyl;
'{ ' represents the attachment to ring B;
'[' represents the attachment to ring P; ring B is sele
wherein, R19 is selected from hydrogen, halogen, hydroxyl, -N(Ci_6 alkyl)2, -NH(Ci_6 alkyl), - Ci-6 haloalkyl, -C2-6 alkenyl, -C2-6 alkynyl, -OCi_6 alkyl, -O C3-6 alkenyl, -O C3-6 alkynyl,-S- Ci-6 alkyl, -CN, -CO-Ci-4 alkyl, -CO— C3-6 cycloalkyl, -COOH, -CONH2, -CON(Ci-6 alkyl)2, - CONH(d_6 alkyl), -NHC(O) -d_4 alkyl, -N(C1 alkyl)C(O) d_4 alkyl and when R19 is -OC3-6 alkenyl or -O C3-6 alkynyl the double and triple bond, respectively, is not on the carbon directly attached to the oxygen atom; Yi, Y2, Y3 and Y4 are independently and appropriately selected from CH, NR20, O, or S; wherein, R20 is selected from hydrogen, -C1-3 alkyl and -C4-6 cycloalkyl;
r is an integer selected from 1 to 3;
'{ ' represents the position of attachment to ring A;
'[' represents the position of attachment to Z;
Z is a bond or is selected from -0-, -S-, -NH-, -N(CM alkyl)-, -N(d_6 alkyl)CO-, -C(0)NH-, -C(Ci-2 alkyl)2-, -C(O)-, -S(O)-, -S(0)2-, -CH20-, -OCH2-, -CH=CH-, -CH2-, -N(CH2CN)- and -NHC(O)-; D is a ring containing 5 to 13 membered aryl, fused aryl, heteroaryl, fused heteroaryl, saturated or unsaturated monocyclic, bicyclic or tricyclic carbocyclic ring containing 0-3 heteroatoms selected from nitrogen, oxygen and sulfur; optionally substituted with Ci_3 alkyl; or D is selected from -Ci-6 alkyl, -C3-6 cycloalkyl, -C1-4 alkyl-0-C2 alkyl, -C3-6 cyclolkyl-O- C2_4 alkyl, -C1-4 alkyl-0-C3-6 cycloalkyl, -Ci_4 haloalkyl-0-C2 alkyl, -C1-4 haloaikyl-S-C2_4 alkyl and -C1-4 alkyl-S-C2_4 alkyl; with the proviso that when:
ring B and D are phenyl;
Z is selected from -0-, -S-, -NH-, -N(Ci-4 alkyl)- or -C(O)-;
Ring A is selected from
and V is hydrogen then:
R2 is not selected from -C(0)R5, -C(S)R5, -S02R5, -SOR5 wherein R5 is Ry; and
2. The compound of Formula I as in claim 1 wherein,
Rj is hydrogen; selected from -CN, -C(0)R5, -C(S)R5, -S02R5 wherein,
R5 is selected from a group comprising Rs>, -Ci_6 alkyl, -C3-6 cycloalkyl, -Ci_3 heterocycloalkenylalkyl, NH C6-10 aryl, C5.10 heteroaryl containing 1-3 heteroatoms selected from oxygen, nitrogen or sulfur, wherein
alkyl and cycloalkyl are optionally further substituted with CN group; and aryl, heteroaryl and heterocycloalkenylalkyl are optionally substituted with one or more halogen;
R5' is selected from a group consisting of -C2-6 alkenyl, -C2-6 alkynyl, -CH=CH-(CH2)n- COOR6 and -C(=CH2)CH2COOR6; n is an integer and at each occurrence is selected from 0 to 3;
R6 is selected from hydrogen, -Ci_3 alkyl; or R2 is a 6 membered heteroaryl ring substituted with at least one group selected from halogen, C3-6 alkenyl, C3-6 alkynyl and -SH group; and optionally further substituted with one or more groups selected from S-C2 alkenyl, -COOH, -OCi-6 alkyl, -CON(-Ci-3 alkyl), -NO2 and -CONH2; or R2 is a group selected from following moieties
wherein, Rg and R are independently selected from hydrogen and Ci_6 alkyl, or Rg and R together forms 4 to 10 membered mono-cyclic ring optionally substituted with one or more d_3 alkyl group;
EWG is-C(0)Od_3 alkyl;
OR
wherein, Rn and Rj2 are independently selected from hydrogen, Ci_6 alkyl, C2-6 alkenyl, - COOR and -C4_6 heteroaryl containing 1-3 heteroatoms selected from oxygen, nitrogen or
sulfur; wherein R is hydrogen or Ci_6 alkyl and the heteroaryl is optionally substituted with one or more halogen atoms; or Rn and Rj2 together forms substituted or unsubstituted 5 to 6 membered saturated or unsaturated mono carbocyclic ring or a 5 to 6 membered heterocyclic ring containing 1 to 2 heteroatoms selected from oxygen and nitrogen; and the carbocyclic and the heterocyclic rings are optionally further substituted with one or more halogen atoms;
ring P is 4 to 6 membered saturated monocyclic, ring containing one additional heteroatom selected from nitrogen;
ring A is a heterocycle selected from followin moieties
ring B is phenyl;
Z is selected from -0-, -C(0)NH- and -NHC(O)-;
D is phenyl or pyridine ring unsubstituted or substituted with Ci_3 alkyl.
3. The compound of Formula I as in claims 1 and 2, wherein
Rj is hydrogen; R2 is -C(0)Rs or a 6 membered heteroaryl ring substituted with one or more halogen radical; or R2 is a group selected from following moieties
wherein, Rg and R are independently selected from hydrogen and Ci_6 alkyl, or Rg and R together forms 4 to 10 membered mono-cyclic ring optionally substituted with one or more Ci-3 alkyl group;
Z is -0-; and D is a phenyl ring.
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