WO2017133036A1 - Method for breeding fungi by using rotten wood - Google Patents

Method for breeding fungi by using rotten wood Download PDF

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Publication number
WO2017133036A1
WO2017133036A1 PCT/CN2016/074602 CN2016074602W WO2017133036A1 WO 2017133036 A1 WO2017133036 A1 WO 2017133036A1 CN 2016074602 W CN2016074602 W CN 2016074602W WO 2017133036 A1 WO2017133036 A1 WO 2017133036A1
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wood
fungus
rot
decayed
pile
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PCT/CN2016/074602
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French (fr)
Chinese (zh)
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程雪娇
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程雪娇
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/50Inoculation of spawn
    • A01G18/55Forming inoculation holes

Definitions

  • the present invention relates to the field of fungal culture, and more particularly to a method for breeding fungi using rot.
  • Rot wood refers to trees that are rotten and disintegrated. In the prior art, the wood is generally discarded or incinerated, and on the one hand, incomplete combustion is caused, resulting in air pollution and environmental hazards; on the other hand, the value of the wood is not fully utilized.
  • Saprophytic fungi are a type of fungus that is a succulent lifestyle. It grows on decaying trees or wood. It has no chlorophyll and cannot carry out photosynthesis. It can only rely on cellulose or semi-fibres in wood or herb. Substances such as lignin, lignin and organic nitrogen absorb the nutrients needed for their life activities. Such as mushrooms, fungus, enoki mushroom, Hericium erinaceus, honey fungus, etc., usually can be used for food and medicinal purposes, because of nutrient-rich, delicious taste, and significant efficacy, it is widely promoted, but often because of improper planting methods, resulting in low fungus production .
  • the present invention proposes a method for breeding fungi using rot, comprising the following steps:
  • the decay of wood placed inorganic salt solution soak 30-35 days; wherein the inorganic salt solution include the following: KHCO 0.40-0.6gL 1 of MgSO 3, 0.30-0.35gL 1 to 4 ⁇ 7 ⁇ 2 0, 0.02-0.03gL 1 of KH 2 P0 4 , 0.1-0.15g 'L 1 of CaCl 2 , 0.3-0.4g'L 1 of NaNO 2 , 0.4-0.5g'L 1 of (NH4)
  • the strain in the step S50 is a saprophytic fungus species.
  • the saprophytic fungus is one of sulfur bacteria, fungus, Hericium erinaceus, and honey fungus.
  • step S10 the rinsed decayed wood is placed in a 2-3% sodium hydroxide solution for 20-30 hours, and the decayed wood is removed and rinsed again with water; Immerse the decayed wood in physiological saline for 20-30 hours.
  • the decaying wood is made of wood, and the decomposing process comprises the following steps:
  • the wood is one of eucalyptus, eucalyptus, and poplar trees.
  • the fungus is cultured in a specific environment (corrosive wood), and the fungus is activated and propagated under the condition of ensuring the nutrient content of the fungus, thereby achieving a high yield effect;
  • the present invention provides a method of breeding a fungus using rot, which comprises the following steps:
  • the washed decayed wood is soaked for 20-30 hours with a 2-3% sodium hydroxide solution to neutralize the decayed wood.
  • Attached hydrochloric acid solution or other acidic substances After soaking with sodium hydroxide solution, pick up the decayed wood and rinse again with water to further remove impurities from the decayed wood. After that, the decayed wood is placed in physiological saline for 20-30 hours and then used.
  • Step S30 placing the decaying wood into the inorganic salt solution for 30-35 days; wherein, the inorganic salt solution comprises the following materials: 0.40-0.6 g L 1 of KHC0 3 , 0.30-0.35 g of L 1 of MgSO 4 ⁇ 7 ⁇ 2
  • Step S50 The decayed wood treated by the above steps S10 and S30 is placed in a dark and humid culture chamber, and the strain is inserted into the aperture of the decayed wood, and pressed with a wooden stick. Maintain the temperature in the culture chamber 15-2 2 ° C, oxygen concentration 5-8mg / L. The fungus community is formed on the decayed wood, that is, the fungus operation of the rotted wood is completed.
  • the strain is a saprophytic fungus species, and specifically: sulfur bacteria, fungus, hericium, honey fungus, and the like.
  • wood can also be used as a raw material to prepare decayed wood, and the wood head as a raw material can be eucalyptus, eucalyptus, and poplar.
  • the specific process is:
  • a temperature sensor or a temperature monitor is disposed at a center of each wood pile for sensing The temperature at the center of the wood pile.
  • the temperature at the center of the pile rises to 55 ° C - 65 ° C; preferably, when the temperature at the center of the pile rises to 60 ° C, the corresponding pile of wood is disturbed, turned over, and white
  • the rot fungus; the white rot fungus and the wood are thoroughly mixed, and the wood pile is again built, and continuously piled for 14-16 days, preferably, for 15 days.
  • the method for using the wood and the decayed wood for fungal culture utilizes the decayed wood to create a natural parasitic environment for the fungus, thereby improving the activity and reproduction speed of the fungus, saving manpower and material resources, thereby reducing the cost.

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Chemical And Physical Treatments For Wood And The Like (AREA)

Abstract

A method for breeding fungi by using rotten wood comprises the steps of: soaking rotten wood in a hydrochloric acid solution with the concentration of 10% to 15% for 18 to 20 hours, and taking out the rotten wood and washing the rotten wood clean after multiple apertures are formed in the rotten wood; then placing the rotten wood in an inorganic salt solution for being soaked for 30 to 35 days; then, placing the rotten wood in a dark and moist cultivation chamber, inoculating strains into the apertures of the rotten wood and carrying out pressing by using a wooden rod; and when a fungal community is formed on the rotten wood, completing operations of breeding fungi by using rotten wood.

Description

发明名称:一种利用腐木养殖真菌的方法  Title of the invention: A method for breeding fungi using rot
技术领域  Technical field
[0001] 本发明涉及真菌养殖领域, 特别涉及一种利用腐木养殖真菌的方法。  [0001] The present invention relates to the field of fungal culture, and more particularly to a method for breeding fungi using rot.
背景技术  Background technique
[0002] 腐木, 是指木材糟烂、 解体的树木。 现有技术中, 腐木一般被废弃或经过焚烧 处理, 一方面容易出现燃烧不完全, 造成空气污染和环境隐患; 另一方面, 腐 木的价值未得到充分利用。  [0002] Rot wood refers to trees that are rotten and disintegrated. In the prior art, the wood is generally discarded or incinerated, and on the one hand, incomplete combustion is caused, resulting in air pollution and environmental hazards; on the other hand, the value of the wood is not fully utilized.
[0003] 腐生性真菌是一类营腐生生活方式的真菌, 生长在腐烂的树木或木材上, 自身 没有叶绿素, 不能进行光合作用, 只能靠分解木材中或草本植物中的纤维素、 半纤维素、 木质素和有机氮等物质, 从中吸取其生命活动所需要的营养成分。 如香菇、 木耳、 金针菇、 猴头菌、 蜜环菌等, 通常可做食用药用, 因营养丰富 、 味道鲜美、 药效显著而被广泛推广种植, 但常常因为种植的方法不对, 导致 真菌低产。  [0003] Saprophytic fungi are a type of fungus that is a succulent lifestyle. It grows on decaying trees or wood. It has no chlorophyll and cannot carry out photosynthesis. It can only rely on cellulose or semi-fibres in wood or herb. Substances such as lignin, lignin and organic nitrogen absorb the nutrients needed for their life activities. Such as mushrooms, fungus, enoki mushroom, Hericium erinaceus, honey fungus, etc., usually can be used for food and medicinal purposes, because of nutrient-rich, delicious taste, and significant efficacy, it is widely promoted, but often because of improper planting methods, resulting in low fungus production .
技术问题  technical problem
问题的解决方案  Problem solution
技术解决方案  Technical solution
[0004] 为了解决现有技术的问题, 本发明提出一种利用腐木养殖真菌的方法, 包括如 下步骤:  In order to solve the problems of the prior art, the present invention proposes a method for breeding fungi using rot, comprising the following steps:
[0005] S10, 将腐朽木浸泡在浓度为 10-15%的盐酸溶液持续 18-20个小吋, 直至盐酸溶 液将腐朽木腐蚀形成多个便于真菌生长的孔径; 捞出腐朽木, 并用水冲洗干净  [0005] S10, immersing the decayed wood in a hydrochloric acid solution having a concentration of 10-15% for 18-20 hours until the hydrochloric acid solution corrodes the decayed wood to form a plurality of pores suitable for fungal growth; removing the decayed wood, and using water Rinse clean
[0006] S30, 将腐朽木放置到无机盐溶液中浸泡 30-35天; 其中, 所述无机盐溶液包括 以下物质: 0.40-0.6g.L 1的 KHCO 3, 0.30-0.35g-L 1的 MgSO 4·7Η 20, 0.02-0.03g-L 1的 KH 2P0 4, 0.1-0.15g'L 1的 CaCl 2, 0.3-0.4g'L 1的 NaNO 2, 0.4-0.5g'L 1的 (NH4)[0006] S30, the decay of wood placed inorganic salt solution soak 30-35 days; wherein the inorganic salt solution include the following: KHCO 0.40-0.6gL 1 of MgSO 3, 0.30-0.35gL 1 to 4 · 2 0, 0.02-0.03gL 1 of KH 2 P0 4 , 0.1-0.15g 'L 1 of CaCl 2 , 0.3-0.4g'L 1 of NaNO 2 , 0.4-0.5g'L 1 of (NH4)
2S0 4; 2 S0 4 ;
[0007] S50, 将经过上述步骤 Sl、 S3处理过的腐朽木放置在阴暗潮湿的培养室中, 将 菌种接入腐朽木的孔径内, 并用木棒进行按压; 保持培养室内的温度 15-22°C、 氧气浓度 5-8mg/L。 [0007] S50, placing the decayed wood treated by the above steps S1, S3 in a dark and humid culture chamber, The strain is inserted into the pore size of the decaying wood and pressed with a wooden stick; the temperature in the culture chamber is maintained at 15-22 ° C, and the oxygen concentration is 5-8 mg/L.
[0008] 优选地, 步骤 S50中所述菌种为腐生性真菌菌种。 [0008] Preferably, the strain in the step S50 is a saprophytic fungus species.
[0009] 优选地, 所述腐生性真菌为硫磺菌、 木耳、 猴头菌、 蜜环菌中的一种。 [0009] Preferably, the saprophytic fungus is one of sulfur bacteria, fungus, Hericium erinaceus, and honey fungus.
[0010] 优选地, 在步骤 S10中, 冲洗干净后的腐朽木再放入质量分数为 2-3%的氢氧化 钠溶液中浸泡 20-30小吋, 捞出腐朽木再次用水冲洗; 之后, 将腐朽木浸入生理 盐水中保持 20-30小吋。 [0010] Preferably, in step S10, the rinsed decayed wood is placed in a 2-3% sodium hydroxide solution for 20-30 hours, and the decayed wood is removed and rinsed again with water; Immerse the decayed wood in physiological saline for 20-30 hours.
[0011] 优选地, 所述腐朽木由木头腐熟而成, 且腐熟过程包括如下步骤: [0011] Preferably, the decaying wood is made of wood, and the decomposing process comprises the following steps:
[0012] S51 , 将木头用水浸泡至含水量达到 40<¾-50<¾, 且所述水中添加有尿素; [0012] S51, soaking the wood with water to a water content of 40<3⁄4-50<3⁄4, and adding urea to the water;
[0013] S53 , 将浸泡后的木头交错叠放以形成多个木头堆, 当监测所述木头堆的中心 处的温度上升至 55°C-65°C吋, 将对应的木头堆打乱, 翻堆, 并加入白腐菌剂; 使白腐菌剂和木头充分混合后再次建木头堆, 并持续堆放 14-16天; [0013] S53, stacking the soaked woods in a staggered manner to form a plurality of wood piles, and when monitoring the temperature at the center of the pile of wood to rise to 55 ° C - 65 ° C, the corresponding pile of wood is disturbed, Turn over the pile and add white rot fungus; make the white rot fungus and wood fully mixed and build the wood pile again, and continue stacking for 14-16 days;
[0014] S55 , 再次将木头堆打乱, 翻堆, 并重建木头堆, 且持续堆放 14-16天腐熟完成 [0014] S55, again disrupting the pile of wood, turning over the pile, and rebuilding the pile of wood, and continuing to stack 14-16 days of maturity
[0015] 优选地, 所述木头为蕖树木、 榆树木、 杨树木中的一种。 [0015] Preferably, the wood is one of eucalyptus, eucalyptus, and poplar trees.
发明的有益效果  Advantageous effects of the invention
有益效果  Beneficial effect
[0016] 本发明利用腐木养殖真菌的有益效果是:  [0016] The beneficial effects of the present invention using rotted wood to culture fungi are:
[0017] 1、 将真菌在特定的环境 (腐朽木) 中进行养殖, 在保证真菌营养含量的状况 下提高真菌的成活性及繁殖速度, 从而达到高产的效果;  [0017] 1. The fungus is cultured in a specific environment (corrosive wood), and the fungus is activated and propagated under the condition of ensuring the nutrient content of the fungus, thereby achieving a high yield effect;
[0018] 2、 节省人力物力, 从而降低成本。  [0018] 2. Saving manpower and material resources, thereby reducing costs.
实施该发明的最佳实施例  BEST MODE FOR CARRYING OUT THE INVENTION
本发明的最佳实施方式  BEST MODE FOR CARRYING OUT THE INVENTION
[0019] 应当理解, 此处所描述的具体实施例仅仅用以解释本发明, 并非用于限定本发 明的范围。 [0019] It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the scope of the invention.
[0020] 本发明提出一种利用腐木养殖真菌的方法, 其包括如下步骤:  [0020] The present invention provides a method of breeding a fungus using rot, which comprises the following steps:
[0021] S10, 将腐朽木浸泡在浓度为 10-15%的盐酸溶液持续 18-20个小吋, 使得盐酸溶 液将腐朽木腐蚀形成多个便于真菌生长的孔径; 然后, 捞出腐朽木, 并用水将 腐朽木上残余的盐酸溶液冲洗干净。 [0021] S10, immersing the decayed wood in a hydrochloric acid solution having a concentration of 10-15% for 18-20 hours, causing the hydrochloric acid solution to corrode the decayed wood to form a plurality of pores suitable for fungal growth; and then, removing the decayed wood, And water will Rinse off the residual hydrochloric acid solution on the decayed wood.
[0022] 为了避免腐朽木上附着有残余的盐酸溶液会损伤真菌, 将冲洗后的腐朽木用质 量分数为 2-3%的氢氧化钠溶液浸泡 20-30小吋, 以中和腐朽木上附着的盐酸溶液 或其它酸性物质。 用氢氧化钠溶液浸泡完成后, 捞起腐朽木再次用水冲洗, 以 进一步的去除腐朽木上的杂质。 之后, 将腐朽木放入生理盐水中浸泡 20-30小吋 后待用。 [0022] In order to avoid damage to the fungus by attaching residual hydrochloric acid solution to the decaying wood, the washed decayed wood is soaked for 20-30 hours with a 2-3% sodium hydroxide solution to neutralize the decayed wood. Attached hydrochloric acid solution or other acidic substances. After soaking with sodium hydroxide solution, pick up the decayed wood and rinse again with water to further remove impurities from the decayed wood. After that, the decayed wood is placed in physiological saline for 20-30 hours and then used.
[0023] 步骤 S30, 将腐朽木放置到无机盐溶液中浸泡 30-35天; 其中, 无机盐溶液包括 以下物质: 0.40-0.6g.L 1的 KHC0 3, 0.30-0.35g.L 1的 MgSO 4·7Η 2 [0023] Step S30, placing the decaying wood into the inorganic salt solution for 30-35 days; wherein, the inorganic salt solution comprises the following materials: 0.40-0.6 g L 1 of KHC0 3 , 0.30-0.35 g of L 1 of MgSO 4 ·7Η 2
0, 0.02-0.03g-L 1的 KH 2P0 4, 0.1-0.15g-L 1的 CaCl 2, 0.3-0.4g.L 1的 NaNO 2 , 0.4-0.5§丄 的 (NH4) 2S0 4。 作为优选的方式: 腐朽木需在无机盐溶液中浸泡 30 天。 0, KH of 0.02-0.03gL 1 2 P0 4, CaCl of 0.1-0.15gL 1 2, NaNO 2, 0.4-0.5 § Shang of (NH4) of 0.3-0.4gL 1 2 S0 4. As a preferred method: The decayed wood needs to be soaked in an inorganic salt solution for 30 days.
[0024] 步骤 S50 : 将经过上述步骤 S10、 S30处理过的腐朽木放置在阴暗潮湿的培养室 中, 将菌种接入腐朽木的孔径内, 并用木棒进行按压。 保持培养室内的温度 15-2 2°C、 氧气浓度 5-8mg/L。 待腐朽木上形成真菌群落, 即完成腐木养殖真菌操作。  [0024] Step S50: The decayed wood treated by the above steps S10 and S30 is placed in a dark and humid culture chamber, and the strain is inserted into the aperture of the decayed wood, and pressed with a wooden stick. Maintain the temperature in the culture chamber 15-2 2 ° C, oxygen concentration 5-8mg / L. The fungus community is formed on the decayed wood, that is, the fungus operation of the rotted wood is completed.
[0025] 需要说明的是, 所述菌种为腐生性真菌菌种, 具体可为: 硫磺菌、 木耳、 猴头 菌、 蜜环菌等。 [0025] It should be noted that the strain is a saprophytic fungus species, and specifically: sulfur bacteria, fungus, hericium, honey fungus, and the like.
[0026] 在其它实施方式中, 也可以用木头作为原材料制备腐朽木, 且作为原材料的木 头可为蕖树、 榆树、 杨树。 具体过程为:  In other embodiments, wood can also be used as a raw material to prepare decayed wood, and the wood head as a raw material can be eucalyptus, eucalyptus, and poplar. The specific process is:
[0027] S51 , 将木头用水充分浸泡, 使木头含水量达到 40<¾-50<¾, 并且上述水中添加 有尿素;  [0027] S51, the wood is fully soaked with water to make the water content of the wood reach 40<3⁄4-50<3⁄4, and the above water is added with urea;
[0028] S53 , 将浸泡后的木头交错叠放以形成多个木头堆, 且为了保证每根木头的腐 熟效果, 每一木头堆的中心位置设有温度传感器或温度监测计, 用于感测木头 堆中心处的温度。 当木头堆的中心处的温度上升至 55°C-65°C; 优选地, 当木头 堆的中心处的温度上升至 60°C吋, 将对应的木头堆打乱, 翻堆, 并加入白腐菌剂 ; 使白腐菌剂和木头充分混合后再次建木头堆, 并持续堆放 14-16天, 优选地, 持续堆放 15天。  [0028] S53, stacking the soaked woods to form a plurality of wood piles, and in order to ensure the decomposing effect of each wood, a temperature sensor or a temperature monitor is disposed at a center of each wood pile for sensing The temperature at the center of the wood pile. When the temperature at the center of the pile rises to 55 ° C - 65 ° C; preferably, when the temperature at the center of the pile rises to 60 ° C, the corresponding pile of wood is disturbed, turned over, and white The rot fungus; the white rot fungus and the wood are thoroughly mixed, and the wood pile is again built, and continuously piled for 14-16 days, preferably, for 15 days.
[0029] S57, 再次将木头堆打乱, 翻堆, 并重建木头堆, 且持续堆放 14-16天, 优选地 [0029] S57, again disrupting the pile of wood, turning over the pile, and rebuilding the pile of wood, and continuously stacking for 14-16 days, preferably
, 持续堆放 15天; 此吋, 木头腐熟完成形成腐朽木。 [0030] 本发明的利用木头、 腐朽木进行真菌养殖的方法利用腐朽木为真菌营造天然的 寄生环境, 提高了真菌的成活性及繁殖速度, 节省了人力物力, 从而降低了成 本。 , continued to stack for 15 days; this time, the wood is ripe to complete the formation of decaying wood. [0030] The method for using the wood and the decayed wood for fungal culture utilizes the decayed wood to create a natural parasitic environment for the fungus, thereby improving the activity and reproduction speed of the fungus, saving manpower and material resources, thereby reducing the cost.
[0031] 以上所述仅为本发明的较佳实施例, 并不用以限制本发明, 凡在本发明的精 神和原则之内, 所作的任何修改、 等同替换、 改进等, 均应包含在本发明的保 护范围之内。  The above description is only the preferred embodiment of the present invention, and is not intended to limit the present invention. Any modifications, equivalents, improvements, etc., which are included in the spirit and principles of the present invention, should be included in the present invention. Within the scope of protection of the invention.
[0032]  [0032]

Claims

权利要求书 Claim
一种利用腐木养殖真菌的方法, 其特征在于, 包括如下步骤:A method for breeding fungi using rot, characterized in that it comprises the following steps:
S10, 将腐朽木浸泡在浓度为 10-15%的盐酸溶液持续 18-20个小吋, 直 至盐酸溶液将腐朽木腐蚀形成多个便于真菌生长的孔径; 捞出腐朽木 , 并用水冲洗干净; S10, soak the decayed wood in a hydrochloric acid solution having a concentration of 10-15% for 18-20 hours until the hydrochloric acid solution corrodes the decayed wood to form a plurality of pores suitable for fungal growth; remove the decayed wood and rinse it with water;
S30, 将腐朽木放置到无机盐溶液中浸泡 30-35天; 其中, 所述无机盐 溶液包括以下物质: 0.40-0.6g.L - 1的 KHCO 3, 0.30-0.35g-L - 1的 MgSO 4 •7H 20, 0.02-0.03g'L 1的 KH 2P0 4, 0.1-0.15g'L 1的 CaCl 2, 0.3-0.4g-L - 1的 NaNO 2, 0.4-0.5g-L - 1的 (NH4) 2SO 4; S30, placing the decaying wood in an inorganic salt solution for 30-35 days; wherein the inorganic salt solution comprises the following materials: 0.40-0.6 g L -1 of KHCO 3 , 0.30-0.35 g of L - 1 of MgSO 4 • 7H 2 0, 0.02-0.03g'L 1 KH 2 P0 4 , 0.1-0.15g 'L 1 CaCl 2 , 0.3-0.4 g L -1 NaNO 2 , 0.4-0.5 g L -1 (NH4) 2 SO 4 ;
S50, 将经过上述步骤 S10、 S30处理过的腐朽木放置在阴暗潮湿的培 养室中, 将菌种接入腐朽木的孔径内, 并用木棒进行按压; 保持培养 室内的温度 15-22°C、 氧气浓度 5-8mg/L。  S50, placing the decayed wood treated by the above steps S10 and S30 in a dark and humid culture chamber, inserting the strain into the aperture of the decaying wood, and pressing with a wooden stick; maintaining the temperature in the culture chamber at 15-22 ° C , oxygen concentration of 5-8mg / L.
如权利要求 1所述的利用腐木养殖真菌的方法, 其特征在于, 在步骤 S 10中, 冲洗干净后的腐朽木再放入质量分数为 2-3%的氢氧化钠溶液 中浸泡 20-30小吋, 捞出腐朽木再次用水冲洗; 之后, 将腐朽木浸入 生理盐水中保持 20-30小吋。 The method for cultivating a fungus using rot, according to claim 1, wherein in step S10, the washed rotted wood is further immersed in a sodium hydroxide solution having a mass fraction of 2-3%. 30 吋, remove the decayed wood and rinse again with water; after that, immerse the decayed wood in physiological saline for 20-30 hours.
如权利要求 1所述的利用腐木养殖真菌的方法, 其特征在于, 步骤 S50 中所述菌种为腐生性真菌菌种。 The method for breeding a fungus using a rot, according to claim 1, wherein the species in the step S50 is a saprophytic fungus species.
一种如权利要求 3所述的利用腐木养殖真菌的方法, 其特征在于, 所 述腐生性真菌为硫磺菌、 木耳、 猴头菌、 蜜环菌中的一种。 A method for breeding a fungus using a rot, according to claim 3, wherein the saprophytic fungus is one of sulfur bacteria, fungus, Hericium erinaceus, and honey fungus.
如权利要求 1所述的利用腐木养殖真菌的方法, 其特征在于, 所述腐 朽木由木头腐熟而成, 且腐熟过程包括如下步骤: A method of cultivating a fungus using rot, according to claim 1, wherein the rotted wood is made of wood, and the decomposing process comprises the following steps:
S53 , 将浸泡后的木头交错叠放以形成多个木头堆, 当监测所述木头 堆的中心处的温度上升至 55°C-65°C吋, 将对应的木头堆打乱, 翻堆 , 并加入白腐菌剂; 使白腐菌剂和木头充分混合后再次建木头堆, 并 持续堆放 14-16天; S55, 再次将木头堆打乱, 翻堆, 并重建木头堆, 且持续堆放 14-16 天腐熟完成。 S53, stacking the soaked woods to form a plurality of wood piles. When the temperature at the center of the pile is monitored to rise to 55 ° C - 65 ° C, the corresponding piles of wood are disturbed and turned over. And adding white rot fungi; make the white rot fungus and wood fully mixed and then build the wood pile again, and continue to stack for 14-16 days; S55, again disrupted the pile of wood, turned over the pile, and rebuilt the pile of wood, and continued stacking for 14-16 days.
[权利要求 6] 如权利要求 5所述的利用腐木养殖真菌的方法, 其特征在于, 所述木 头为蕖树木、 榆树木、 杨树木中的一种。  [Claim 6] The method for breeding a fungus using rot, according to claim 5, wherein the wood head is one of eucalyptus, eucalyptus, and poplar trees.
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103688758A (en) * 2013-12-15 2014-04-02 张爱美 Original ecological cultivation method for trametes
CN104129847A (en) * 2014-06-06 2014-11-05 武汉风林环境科技有限公司 Method for one-step denitrification by utilizing wood and decayed wood for enrichment of denitrification environment microbial communities
CN104170652A (en) * 2014-08-21 2014-12-03 两当县云屏乡裕通猪苓产销专业合作社 Method for cultivating grifola by filling cut-log
CN104488542A (en) * 2013-12-05 2015-04-08 广西仙草堂制药有限责任公司 Ganoderma lucidum cultivation method
CN104641934A (en) * 2015-01-12 2015-05-27 无锡双喜金阳生物科技有限公司 Method for culturing dendrobium candidum on rotted wood

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3169737B2 (en) * 1993-03-30 2001-05-28 寳酒造株式会社 Shiitake mushroom bed cultivation method
CN101392246A (en) * 2008-10-29 2009-03-25 东北电力大学 Method for immobilizing white rot fungi by using bacteria cellulose film as vector
CN102491843B (en) * 2011-12-06 2013-04-03 湖南科技大学 Method for preparing ecological cultivation matrix for greening roofs

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104488542A (en) * 2013-12-05 2015-04-08 广西仙草堂制药有限责任公司 Ganoderma lucidum cultivation method
CN103688758A (en) * 2013-12-15 2014-04-02 张爱美 Original ecological cultivation method for trametes
CN104129847A (en) * 2014-06-06 2014-11-05 武汉风林环境科技有限公司 Method for one-step denitrification by utilizing wood and decayed wood for enrichment of denitrification environment microbial communities
CN104170652A (en) * 2014-08-21 2014-12-03 两当县云屏乡裕通猪苓产销专业合作社 Method for cultivating grifola by filling cut-log
CN104641934A (en) * 2015-01-12 2015-05-27 无锡双喜金阳生物科技有限公司 Method for culturing dendrobium candidum on rotted wood

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