WO2017099494A8 - Composition d'édition génomique comprenant une cpf1 et son utilisation - Google Patents

Composition d'édition génomique comprenant une cpf1 et son utilisation Download PDF

Info

Publication number
WO2017099494A8
WO2017099494A8 PCT/KR2016/014379 KR2016014379W WO2017099494A8 WO 2017099494 A8 WO2017099494 A8 WO 2017099494A8 KR 2016014379 W KR2016014379 W KR 2016014379W WO 2017099494 A8 WO2017099494 A8 WO 2017099494A8
Authority
WO
WIPO (PCT)
Prior art keywords
genome editing
cpf1
editing composition
composition
technique
Prior art date
Application number
PCT/KR2016/014379
Other languages
English (en)
Korean (ko)
Other versions
WO2017099494A1 (fr
Inventor
김진수
허준호
김대식
김정은
김경미
김혜란
구태영
Original Assignee
기초과학연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 기초과학연구원 filed Critical 기초과학연구원
Publication of WO2017099494A1 publication Critical patent/WO2017099494A1/fr
Publication of WO2017099494A8 publication Critical patent/WO2017099494A8/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne une composition d'édition génomique comprenant une Cpf1, un procédé d'édition génomique l'utilisant et une technique de préparation d'organismes eucaryotes transformés.
PCT/KR2016/014379 2015-12-08 2016-12-07 Composition d'édition génomique comprenant une cpf1 et son utilisation WO2017099494A1 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
KR20150174212 2015-12-08
KR10-2015-0174212 2015-12-08
US201662299043P 2016-02-24 2016-02-24
US62/299,043 2016-02-24
KR10-2016-0036381 2016-03-25
KR20160036381 2016-03-25

Publications (2)

Publication Number Publication Date
WO2017099494A1 WO2017099494A1 (fr) 2017-06-15
WO2017099494A8 true WO2017099494A8 (fr) 2017-08-10

Family

ID=59013788

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2016/014379 WO2017099494A1 (fr) 2015-12-08 2016-12-07 Composition d'édition génomique comprenant une cpf1 et son utilisation

Country Status (2)

Country Link
KR (2) KR101897213B1 (fr)
WO (1) WO2017099494A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11999952B2 (en) 2021-04-21 2024-06-04 Toolgen Incorporated Artificially-manipulated neovascularization regulatory system

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT3066201T (pt) 2013-11-07 2018-06-04 Massachusetts Inst Technology Métodos e composições relacionadas com crispr com arng reguladores
WO2016073990A2 (fr) 2014-11-07 2016-05-12 Editas Medicine, Inc. Procédés pour améliorer l'édition génomique médiée par crispr/cas
EP3294896A1 (fr) 2015-05-11 2018-03-21 Editas Medicine, Inc. Systèmes crispr/cas9 optimisés et procédés d'édition de gènes dans des cellules souches
CN116334142A (zh) 2015-06-09 2023-06-27 爱迪塔斯医药公司 用于改善移植的crispr/cas相关方法和组合物
AU2016326711B2 (en) 2015-09-24 2022-11-03 Editas Medicine, Inc. Use of exonucleases to improve CRISPR/Cas-mediated genome editing
EP3433363A1 (fr) 2016-03-25 2019-01-30 Editas Medicine, Inc. Systèmes d'édition de génome comprenant des molécules d'enzyme modulant la réparation et leurs procédés d'utilisation
US11236313B2 (en) 2016-04-13 2022-02-01 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
US11123409B2 (en) * 2016-07-28 2021-09-21 Institute For Basic Science Method of treating or preventing eye disease using Cas9 protein and guide RNA
SG11201901306XA (en) * 2016-08-19 2019-03-28 Toolgen Inc Artificially engineered angiogenesis regulatory system
US11499151B2 (en) 2017-04-28 2022-11-15 Editas Medicine, Inc. Methods and systems for analyzing guide RNA molecules
JP2020524497A (ja) 2017-06-09 2020-08-20 エディタス・メディシン，インコーポレイテッド 操作されたｃａｓ９ヌクレアーゼ
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
EP3652312A1 (fr) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systèmes et procédés d'intégration ciblée et d'édition du génome et détection de celle-ci à l'aide de sites d'amorçage intégrés
WO2019103442A2 (fr) * 2017-11-21 2019-05-31 한국생명공학연구원 Composition d'édition génomique utilisant un système crispr/cpf1 et son utilisation
EP3744844A4 (fr) * 2018-01-23 2021-10-20 Institute for Basic Science Arn guide simple étendu et utilisation associée
WO2019173942A1 (fr) * 2018-03-12 2019-09-19 Nanjing Bioheng Biotech Co., Ltd Arn guide chimérique modifié et ses utilisations
KR102177174B1 (ko) 2018-05-18 2020-11-10 울산대학교 산학협력단 Pde6b 유전자가 결손된 망막 변성 동물 모델 및 이의 제조 방법
WO2020028729A1 (fr) 2018-08-01 2020-02-06 Mammoth Biosciences, Inc. Compositions de nucléase programmable et leurs méthodes d'utilisation
US20210292722A1 (en) * 2018-08-09 2021-09-23 G+Flas Life Sciences Novel crispr-associated protein and use thereof
CA3109083A1 (fr) 2018-08-09 2020-02-13 G+Flas Life Sciences Compositions et procedes de modification du genome avec des proteines cas12a
CN109666684A (zh) * 2018-12-25 2019-04-23 北京化工大学 一种CRISPR/Cas12a基因编辑***及其应用
WO2020142754A2 (fr) 2019-01-04 2020-07-09 Mammoth Biosciences, Inc. Améliorations de nucléase programmable ainsi que compositions et méthodes d'amplification et de détection d'acide nucléique
KR102493904B1 (ko) 2019-12-13 2023-01-31 한국생명공학연구원 EeCpf1에 의해 IL2Rg 유전자가 돌연변이된 면역부전 동물모델 및 그 제조방법
KR102551876B1 (ko) 2019-12-18 2023-07-05 한국생명공학연구원 Cpf1 및 키메릭 DNA-RNA 가이드를 포함하는 유전체 교정 또는 발현 억제용 조성물
KR102471698B1 (ko) * 2020-03-24 2022-11-28 연세대학교 산학협력단 신규한 가이드 rna 및 이를 이용한 코로나바이러스감염증 2019를 진단하는 방법
WO2021194172A1 (fr) * 2020-03-24 2021-09-30 연세대학교 산학협력단 Nouvel arn guide et méthode de diagnostic d'une infection à coronavirus 2019 faisant appel à celui-ci
CN113373170A (zh) * 2021-04-29 2021-09-10 江西农业大学 一种pFnCpfAb/pCrAb双质粒***及其应用
CN113969281B (zh) * 2021-12-24 2022-04-01 汕头大学 经修饰的CrRNA片段及非洲猪瘟病毒试剂盒
KR20240020336A (ko) 2022-08-04 2024-02-15 성균관대학교산학협력단 PAM 서열에 비의존적인 돌연변이 Cas9 단백질

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116064532A (zh) * 2012-10-23 2023-05-05 基因工具股份有限公司 用于切割靶dna的组合物及其用途
AU2014361781B2 (en) 2013-12-12 2021-04-01 Massachusetts Institute Of Technology Delivery, use and therapeutic applications of the CRISPR -Cas systems and compositions for genome editing
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11999952B2 (en) 2021-04-21 2024-06-04 Toolgen Incorporated Artificially-manipulated neovascularization regulatory system

Also Published As

Publication number Publication date
KR20170068400A (ko) 2017-06-19
WO2017099494A1 (fr) 2017-06-15
KR101897213B1 (ko) 2018-09-11
KR20180028996A (ko) 2018-03-19
KR101958437B1 (ko) 2019-03-15

Similar Documents

Publication Publication Date Title
WO2017099494A8 (fr) Composition d'édition génomique comprenant une cpf1 et son utilisation
WO2017031360A8 (fr) Capture d'acides nucléiques à l'aide d'un système utilisant une nucléase guidée par des acides nucléiques
PL3577110T3 (pl) Związki podstawione 8-oksetan-3-ylo-3,8-diazabicyklo[3.2.1]oktan-3-ylem jako inhibitory hiv
EP3691747A4 (fr) Compositions et procédés d'édition des arn
EP3715461A4 (fr) Composition d'édition génomique utilisant un système crispr/cpf1 et son utilisation
WO2015200902A8 (fr) Endophytes, compositions associées et leurs méthodes d'utilisation
EP3271021A4 (fr) Édition génique basée sur le système crispr/endonucléase à induction par tat
EP3427822A4 (fr) Catalyseur pour la préparation d'acide 2,5-furancarboxylique et procédé de préparation d'acide 2,5-furancarboxylique au moyen du catalyseur
EP3456825A4 (fr) Procédé d'édition du génome d'une plante
WO2014144761A3 (fr) Augmentation de la spécificité pour la modification du génome à guidage arn
IL282178A (en) Products, uses and methods
EP3322526A4 (fr) Catalyseurs multimétalliques contenant du cuivre améliorés et procédé d'utilisation de ceux-ci pour produire du 1,2-propane-diol d'origine biologique
EP3594312A4 (fr) Composition, procédé pour sa production et utilisation associée
WO2016108927A3 (fr) Compositions inhibitrices de nitrification et procédés de préparation de celles-ci
EP3552017A4 (fr) Composés, compositions et méthodes
WO2016033437A3 (fr) Polyéthers, polyamines, polythioéthers, et leurs procédés de fabrication
EP3800994A4 (fr) Édition de génome dans des plantes
GB202017595D0 (en) Compositions, and methods and uses relating thereto
IL284800A (en) Methods, uses and compositions
GB201912024D0 (en) Methods, products & Uses relating thereto
EP3366689A4 (fr) Composé à base de métal de transition, composition catalytique le comprenant, et procédé de préparation de polyoléfine l'utilisant
GB201813648D0 (en) Methods, products & uses relating thereto
EP3199526A4 (fr) Intermédiaire utile dans la synthèse de la paroxétine, procédé de préparation de l'intermédiaire et utilisations associées
EP3480188A4 (fr) Procédé de préparation d'un inhibiteur de l'échangeur 1 d'anions urate
GB201912021D0 (en) Methods, products & Uses relating thereto

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 16873359

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase in:

Ref country code: DE

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC (EPO FORM 1205A DATED 02/10/2018)

122 Ep: pct application non-entry in european phase

Ref document number: 16873359

Country of ref document: EP

Kind code of ref document: A1