WO2017099494A8 - Genome editing composition comprising cpf1, and use thereof - Google Patents

Genome editing composition comprising cpf1, and use thereof Download PDF

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Publication number
WO2017099494A8
WO2017099494A8 PCT/KR2016/014379 KR2016014379W WO2017099494A8 WO 2017099494 A8 WO2017099494 A8 WO 2017099494A8 KR 2016014379 W KR2016014379 W KR 2016014379W WO 2017099494 A8 WO2017099494 A8 WO 2017099494A8
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WO
WIPO (PCT)
Prior art keywords
genome editing
cpf1
editing composition
composition
technique
Prior art date
Application number
PCT/KR2016/014379
Other languages
French (fr)
Korean (ko)
Other versions
WO2017099494A1 (en
Inventor
김진수
허준호
김대식
김정은
김경미
김혜란
구태영
Original Assignee
기초과학연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 기초과학연구원 filed Critical 기초과학연구원
Publication of WO2017099494A1 publication Critical patent/WO2017099494A1/en
Publication of WO2017099494A8 publication Critical patent/WO2017099494A8/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to a genome editing composition comprising Cpf1, a genome editing method using the same, and a technique for preparing transformed eukaryotic organisms.
PCT/KR2016/014379 2015-12-08 2016-12-07 Genome editing composition comprising cpf1, and use thereof WO2017099494A1 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
KR20150174212 2015-12-08
KR10-2015-0174212 2015-12-08
US201662299043P 2016-02-24 2016-02-24
US62/299,043 2016-02-24
KR10-2016-0036381 2016-03-25
KR20160036381 2016-03-25

Publications (2)

Publication Number Publication Date
WO2017099494A1 WO2017099494A1 (en) 2017-06-15
WO2017099494A8 true WO2017099494A8 (en) 2017-08-10

Family

ID=59013788

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2016/014379 WO2017099494A1 (en) 2015-12-08 2016-12-07 Genome editing composition comprising cpf1, and use thereof

Country Status (2)

Country Link
KR (2) KR101897213B1 (en)
WO (1) WO2017099494A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11999952B2 (en) 2021-04-21 2024-06-04 Toolgen Incorporated Artificially-manipulated neovascularization regulatory system

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PT3066201T (en) 2013-11-07 2018-06-04 Massachusetts Inst Technology Crispr-related methods and compositions with governing grnas
WO2016073990A2 (en) 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing
EP3294896A1 (en) 2015-05-11 2018-03-21 Editas Medicine, Inc. Optimized crispr/cas9 systems and methods for gene editing in stem cells
CN116334142A (en) 2015-06-09 2023-06-27 爱迪塔斯医药公司 CRISPR/CAS related methods and compositions for improving transplantation
AU2016326711B2 (en) 2015-09-24 2022-11-03 Editas Medicine, Inc. Use of exonucleases to improve CRISPR/Cas-mediated genome editing
EP3433363A1 (en) 2016-03-25 2019-01-30 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
US11236313B2 (en) 2016-04-13 2022-02-01 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
US11123409B2 (en) * 2016-07-28 2021-09-21 Institute For Basic Science Method of treating or preventing eye disease using Cas9 protein and guide RNA
SG11201901306XA (en) * 2016-08-19 2019-03-28 Toolgen Inc Artificially engineered angiogenesis regulatory system
US11499151B2 (en) 2017-04-28 2022-11-15 Editas Medicine, Inc. Methods and systems for analyzing guide RNA molecules
JP2020524497A (en) 2017-06-09 2020-08-20 エディタス・メディシン，インコーポレイテッド Engineered CAS9 nuclease
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
EP3652312A1 (en) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
WO2019103442A2 (en) * 2017-11-21 2019-05-31 한국생명공학연구원 Genome editing composition using crispr/cpf1 system and use thereof
EP3744844A4 (en) * 2018-01-23 2021-10-20 Institute for Basic Science Extended single guide rna and use thereof
WO2019173942A1 (en) * 2018-03-12 2019-09-19 Nanjing Bioheng Biotech Co., Ltd Engineered chimeric guide rna and uses thereof
KR102177174B1 (en) 2018-05-18 2020-11-10 울산대학교 산학협력단 A retinal degenerated animal model by PDE6B gene deletion and the preparation method thereof
WO2020028729A1 (en) 2018-08-01 2020-02-06 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
US20210292722A1 (en) * 2018-08-09 2021-09-23 G+Flas Life Sciences Novel crispr-associated protein and use thereof
CA3109083A1 (en) 2018-08-09 2020-02-13 G+Flas Life Sciences Compositions and methods for genome engineering with cas12a proteins
CN109666684A (en) * 2018-12-25 2019-04-23 北京化工大学 A kind of CRISPR/Cas12a gene editing system and its application
WO2020142754A2 (en) 2019-01-04 2020-07-09 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
KR102493904B1 (en) 2019-12-13 2023-01-31 한국생명공학연구원 Immunodeficient Animal Model Mutated IL2Rg Gene by EeCpf1 and Method for Producing the same
KR102551876B1 (en) 2019-12-18 2023-07-05 한국생명공학연구원 Composition for Genome Editing or Inhibiting Gene Expression comprising Cpf1 and Chimeric DNA-RNA Guide
KR102471698B1 (en) * 2020-03-24 2022-11-28 연세대학교 산학협력단 Novel guide RNA and method for diagnosing Coronavirus disease 2019
WO2021194172A1 (en) * 2020-03-24 2021-09-30 연세대학교 산학협력단 Novel guide rna and method for diagnosing coronavirus infection 2019 using same
CN113373170A (en) * 2021-04-29 2021-09-10 江西农业大学 pFNCpfAb/pCrAb double-plasmid system and application thereof
CN113969281B (en) * 2021-12-24 2022-04-01 汕头大学 Modified CrRNA fragment and African swine fever virus kit
KR20240020336A (en) 2022-08-04 2024-02-15 성균관대학교산학협력단 Protospacer Adjacent Motif-independent mutant Cas9 protein

Family Cites Families (3)

* Cited by examiner, † Cited by third party
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CN116064532A (en) * 2012-10-23 2023-05-05 基因工具股份有限公司 Composition for cleaving target DNA and use thereof
AU2014361781B2 (en) 2013-12-12 2021-04-01 Massachusetts Institute Of Technology Delivery, use and therapeutic applications of the CRISPR -Cas systems and compositions for genome editing
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11999952B2 (en) 2021-04-21 2024-06-04 Toolgen Incorporated Artificially-manipulated neovascularization regulatory system

Also Published As

Publication number Publication date
KR20170068400A (en) 2017-06-19
WO2017099494A1 (en) 2017-06-15
KR101897213B1 (en) 2018-09-11
KR20180028996A (en) 2018-03-19
KR101958437B1 (en) 2019-03-15

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