WO2017011983A1 - 贻贝粘蛋白用于皮肤和皮肤附属器官的防护 - Google Patents

贻贝粘蛋白用于皮肤和皮肤附属器官的防护 Download PDF

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Publication number
WO2017011983A1
WO2017011983A1 PCT/CN2015/084493 CN2015084493W WO2017011983A1 WO 2017011983 A1 WO2017011983 A1 WO 2017011983A1 CN 2015084493 W CN2015084493 W CN 2015084493W WO 2017011983 A1 WO2017011983 A1 WO 2017011983A1
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Prior art keywords
skin
mussel mucin
mussel
accessory organs
mucin
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PCT/CN2015/084493
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English (en)
French (fr)
Inventor
高敏
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江阴市本特塞缪森生命科学研究院有限公司
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Priority to PCT/CN2015/084493 priority Critical patent/WO2017011983A1/zh
Publication of WO2017011983A1 publication Critical patent/WO2017011983A1/zh

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/12—Aerosols; Foams
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings

Definitions

  • the present invention generally relates to the fields of daily chemical products, cosmetics, medical devices, pharmaceuticals, disinfecting products, health care products, and the like, and relates to the use of mussel mucin or a preparation thereof for protecting skin and skin accessory organs.
  • the skin As the body's first physiological defense line and the largest organ, the skin always participates in the functional activities of the body, maintaining the unity of the body and the natural environment. Any abnormality of the body can also be reflected on the skin surface.
  • the skin consists of the epidermis, dermis, and subcutaneous tissue, and contains many accessory organs such as hair, sweat glands, sebaceous glands, nails, toenails, and blood vessels, lymphatic vessels, nerves, and muscles.
  • the skin has near-perfect physiological protection functions, such as barrier function, sensory function, body temperature regulation, absorption, secretion and excretion, which play an important role in maintaining the health of the body.
  • the skin and its appendages are susceptible to damage from ultraviolet rays, radiation (such as electromagnetic waves, radiation, computer radiation, etc.), hot objects, frostbite caused by low temperature objects, sea water, environmental pollution, and hot dyeing agents.
  • radiation such as electromagnetic waves, radiation, computer radiation, etc.
  • hot objects frostbite caused by low temperature objects, sea water, environmental pollution, and hot dyeing agents.
  • UVB Ultraviolet light
  • UVB UVB in sunlight can cause redness, swelling, burning, burning, edema, skin desquamation and other damage to the skin and skin accessory organs; causing hair yellowing, splitting, easy to break, dry Inelastic, and can not be repaired once the damage.
  • Radiation such as ionizing radiation, including radiotherapy, has a certain therapeutic effect on malignant tumors, but there will be some degree of skin reaction, such as: erythema, burning and itching, desquamation, congestion, edema, blisters, exudation, erosion, Ulcer, skin atrophy, telangiectasia, lymphatic drainage disorders, dark brown spots, pigmentation, etc.
  • Scalding is a problem of skin redness, blisters, peeling, tissue necrosis, ulceration, ulcers, etc. caused by skin contact with objects above body temperature.
  • Frostbite is a local injury to the skin and skin accessory organs caused by cold and dampness. It is cold, pale, hard, numbness or loss, redness and congestion, heat, itching, burning pain, blisters, edema, severe pain, and tissue necrosis.
  • the salt in the sea and the chlorine in the swimming pool can damage the scalp and cause inflammatory reactions such as enlarged pores, and the hair will become dry.
  • the main pollutants in the air are sulfur dioxide, nitrogen oxides, particulate pollutants, and acid rain.
  • Nitrogen-containing pollutants such as sulfur dioxide, nitrous oxide (N 2 O), nitrogen monoxide (NO), nitrogen dioxide (NO 2 ), and nitrogen trioxide (N 2 O 3 ) are highly irritating to human skin.
  • Granular pollutants are large in number and complex in composition. They can be carriers of toxic substances or other pollutants, which can easily cause skin inflammation, large pores, red hair follicles, pigmentation and other problems.
  • Hair dyes have the effect of changing the color of hair, containing toxic and harmful substances such as metals, oxides, p-phenylenediamine, etc., causing scalp allergies, redness, itching and the like.
  • the thioglycolic substances in the perm agent are harmful to the hair, scalp and hair follicles, causing the scales to be damaged, which may cause the hair to become yellow, brittle, detached, tarnished and elastic.
  • Mussel adhesive protein also known as Mytilus edulis foot protein (Mefp)
  • Mefp Mytilus edulis foot protein
  • Mytilus coruscus A special protein secreted by Perna viridis. Mussels are usually attached in groups to the reefs on the coast or to the bottom of the ship, and have the ability to withstand wave impacts in the offshore. In fact, mussels can be attached extremely strongly to the substrate of any material, such as metal, wood, glass, and the like. The main reason for the above characteristics of mussels is that they can form and store this special mucin in the girth of the foot. The mussels release the mucin through the foot silk to a solid surface such as rock to form a water-resistant combination. Fix yourself.
  • Mussel mucin has two structural features: (1) containing lysine, which has a high loading of positive charge; (2) containing 3,4 dihydroxyphenylalanine (DOPA, dopa). The cells and tissues of the human body are negatively charged.
  • Mussel mucin plays a protective and therapeutic role by tightly binding cells and tissues through the electrostatic interaction between its own positive charge and the negative charge of cells and tissues.
  • dopa oxidation produces ortho-dioxins, which can be cross-linked with unoxidized dopa to form a membrane or a network scaffold, which promotes the protein to adhere more closely and firmly to the surface of the human body, thereby protecting.
  • Mussel mucin is a macromolecular protein that is completely degraded in the human body for about 3-10 days. Its ability to attach to cell tissues is excellent, so that mussel mucin can be stabilized locally and continue to function.
  • mussel mucin has the above characteristics, its current application field is very limited.
  • Commercial mussel mucin products are Cell-Tak from BD Biosciences, MAP Trix from Kollodis, Korea, and Hydrogel from Biopolymer, Sweden. These products are either used directly in the form of mussel mucin solution, or are stored as lyophilized powder formulations and dissolved prior to use. Their primary field of application is limited to microscopic cell adhesion and tissue adhesives. Mussel mucin has also been reported for use in the repair of fetal membranes, as a coating against seawater corrosion, and as a drug-loaded stent for the heart.
  • Mussel mucin used herein refers to Mytilus edulis Linnaeus, Mytilus coruscus or Perna viridis from the Mytilidae bivalve mollusc. 11 subclasses of mussel mucin, currently known as purified from marine mussels: mefp1, mefp-2, mefp-3, mefp-4, mefp-5, mefp-6, collagen pre-COL- A mixture of one or more of P, pre-COL-D, pre-COL-NG, foot silk matrix proteins PTMP and DTMP.
  • the mussel mucin used herein may have a pH of from 1.0 to 7.0 in an aqueous solution, and particularly may be in the range of from pH 3.0 to 6.5 to make the therapeutic effect better.
  • the mussel mucin used herein can be obtained by the following preparation method, for example, a method for separating and purifying mussel mucin using mixed adsorption chromatography in Chinese Patent No. ZL200710179491.0, a kind of carboxy using Chinese Patent No. ZL200710179492.5 A method for purifying mussel mucin by methyl ion exchange chromatography, a method for separating and purifying mussel mucin using salting out and dialysis, Chinese Patent No. ZL200910087567.6.
  • the mussel mucin used herein may be in the form of a solution or a lyophilized powder, in particular, the concentration of mussel mucin in the product may be 0.1-15.0 mg/ml, and when the concentration is too low, the effect of mussel mucin Not large, when the concentration is too high, the cytotoxicity may be caused by the high positive charge, which is not conducive to play a protective role.
  • the mussel mucin used herein can also be prepared as a liquid agent by combining it with an excipient.
  • Exemplary mussel mucin fluid The body agent is prepared by dissolving or diluting the mother liquor or lyophilized powder of mussel mucin solution to a certain concentration or pH.
  • the solution for dissolving or diluting may be water, physiological saline, phosphate solution, acetate solution, borate. Solution and so on.
  • the pH of mussel mucin in the final product may be pH 1.0-7.0, and in particular, the therapeutic effect may be better in the range of pH 3.0-6.5.
  • the mussel mucin used herein can also be prepared as a gelling agent in combination with an excipient.
  • An exemplary mussel mucin gel is prepared by mixing a mussel mucin solution or a lyophilized powder with a gel matrix material, which may be selected from the group consisting of cellulose derivatives, carbomers, and seaweeds. Acid salt, tragacanth, gelatin, pectin, carrageenan, gellan gum, starch, xanthan gum, cationic guar gum, agar, non-cellulosic polysaccharide, ethylene polymer, acrylic resin, polyvinyl alcohol or poly One of carboxyvinyl or any combination thereof.
  • the mussel mucin used herein can also be combined with excipients to prepare a gel.
  • An exemplary mussel mucin gel is prepared by mixing a mussel mucin solution or a lyophilized powder with a gel matrix, which may include cellulose derivatives, glycerin, non-cellulosic polysaccharides, propylene glycol. One or any combination thereof.
  • the mussel mucin used herein can also be combined with an excipient to prepare a paste.
  • An exemplary mussel mucin paste is prepared by mixing mussel mucin with a paste matrix material, which may include glycerin, petrolatum, paraffin, and the like.
  • the mussel mucin used herein can also be combined with a matrix material to form a dressing and application for the skin surface.
  • the liquid in the exemplary mussel mucin application may be a mussel mucin solution or a cellulose derivative, carbomer and alginate, tragacanth, gelatin, pectin, carrageenan, Gellan gum, starch, xanthan gum, cationic guar gum, agar, non-cellulosic polysaccharide, ethylene polymer, acrylic resin, polyvinyl alcohol or carbomer, gelatin, fish gelatin, pectin, alginate, glycerin One or more combinations of petrolatum, paraffin, polyethylene glycol, vitamins, glutathione.
  • the above mussel mucin solution or combination is immersed in a matrix material such as gauze, non-woven fabric, silk paper, and the application may be, for example, a wound patch, a mask, an eye mask, a hand mask, a foot film, or the like.
  • the mussel mucin used herein can also be made into a foaming agent.
  • An exemplary mussel mucin foaming agent is prepared by mixing a mussel mucin solution or a lyophilized powder with a foaming agent matrix, which may include hydroxypropylmethylcellulose, gelatin, and polyethylene.
  • a foaming agent matrix which may include hydroxypropylmethylcellulose, gelatin, and polyethylene.
  • the defoaming time of the foaming agent is long, and the action time is prolonged, so that the mussel mucin is more effective.
  • the mussel mucin used herein can be used as a main raw material to prepare a medicine using a pharmaceutically acceptable carrier.
  • Place The drug may be a liquid agent, a gel, a gel, a paste, an applicator, or a foaming agent.
  • the medicament can be used for in vitro use, in particular for external use on the skin.
  • the mussel mucin used herein can be used as a main raw material to prepare a medical device.
  • the term medical device as used herein refers to materials that are used directly or indirectly to the human body and other similar or related items.
  • the medical device may be a liquid agent, a gel, a gel, a paste, an applicator, or a foaming agent.
  • the medical device can be used for in vitro use, in particular for external use on the skin.
  • the mussel mucin used herein can be used as a main raw material to prepare a cosmetic using an auxiliary material which is acceptable in the field of cosmetics.
  • the cosmetic may be a liquid, a gel, a gel, a paste, an applicator, or a foaming agent.
  • the cosmetic can be used for in vitro use, in particular for topical application to the skin.
  • the mussel mucin used herein can be used as a main raw material to prepare a disinfecting product using an excipient which is acceptable in the field of disinfecting products.
  • the term disinfecting product as used herein refers to a disinfectant, a disinfecting device, a sanitary article, and a disposable medical article that chemically, physically, or biologically kill or eliminate pathogenic microorganisms in the environment.
  • the disinfecting product may be a liquid agent, a gel, a gel, a paste, an applicator, or a foaming agent.
  • the disinfecting product can be used for in vitro use, in particular for external use on the skin.
  • the mussel mucin used herein can be used as a main raw material, and a health care product or food can be prepared by using an excipient which is acceptable in the field of health care or food.
  • the health care product or food may be a liquid agent, a gel, a gel, a paste, an applicator, or a foaming agent.
  • the health supplement or food can be used for in vitro use or consumption, in particular for external use on the skin.
  • the mussel mucin used herein can be used as a main raw material to prepare a daily chemical product using an auxiliary material acceptable in the field of daily chemical products.
  • the term daily chemical product as used herein refers to technical chemicals that are used daily, including shampoos, shower gels, and the like.
  • the daily chemical product may be a liquid agent, a gel, a gel, a paste, an applicator, or a foaming agent.
  • the daily chemical product can be used for in vitro use, in particular for external use on the skin.
  • hair care and hair care products can be prepared from the mussel mucin of the present invention for protection of scalp and hair and other accessory organs.
  • Another object of the invention is to provide the use of mussel mucin products in the protection of skin and skin appendages.
  • the present invention provides the use of mussel mucin or a preparation thereof for protecting skin accessory organs such as skin and hair from ultraviolet damage and the like.
  • the present invention provides the use of mussel mucin or a preparation thereof for protecting skin and skin accessory organs from radiation (electromagnetic, radiation, computer radiation, etc.).
  • the present invention provides the use of mussel mucin or a preparation thereof for protecting a skin accessory organ such as skin and hair from a burn caused by a high temperature object or the like.
  • the present invention provides the use of mussel mucin or a preparation thereof for protecting a skin appendage such as skin and hair from frostbite caused by a low temperature object or the like.
  • the present invention provides the use of mussel mucin or a preparation thereof for protecting skin accessory organs such as skin and hair from seawater damage, and for protecting skin accessory organs such as skin and hair from environmental pollution.
  • the present invention provides the use of mussel mucin or a preparation thereof for protecting skin accessory organs such as skin and hair from damage such as hot and dyeing agents.
  • mussel mucin application according to embodiment 1, wherein the mussel mucin may be from a subclass: mefp1, mefp-2, mefp-3, mefp-4, mefp-5, mefp-6, collagen A mixture of one or more of pre-COL-P, pre-COL-D, pre-COL-NG, foot silk matrix proteins PTMP and DTMP.
  • the mussel mucin application according to embodiment 1, wherein the mussel mucin concentration may be from 0.1 to 15.0 mg/ml.
  • Mussel mucin application according to embodiment 1, wherein the mussel mucin in the final product may be in the range of pH 1.0-7.0, in particular in the range of pH 3.0-6.5.
  • skin appendages such as skin and hair from ultraviolet rays, radiation, high temperature objects, low temperature objects, Damage to seawater, environmental pollution, hot and dyed reagents.
  • compositions for protecting skin and skin accessory organs wherein the composition may be a liquid agent, a gel, a gel, a paste, an applicator or a foam. Use of the agent.
  • the mussel mucin application according to embodiment 14, wherein the hair care and hair care product can comprise a conditioner, a hair care essence water, a hair care gel, and the like.
  • Example 1 Application of mussel mucin hydrogel cosmetics in ultraviolet protection (sun protection).
  • the sun protection factor of the mussel mucin hydrogel cosmetic of this example was measured to be SPF 25. It is indicated that the mussel mucin product has the function of protecting the skin from ultraviolet rays.
  • Example 2 Application of mussel mucin liquid cosmetics in ultraviolet protection (sun protection).
  • a mussel mucin solution having a concentration of 10.0 mg/ml was taken, diluted with physiological saline, and 10 mg of vitamin C powder was added to prepare a mussel mucin liquid cosmetic, wherein the mussel mucin concentration was 2 mg/ml.
  • the sun protection factor of the mussel mucinized liquid cosmetic of the present example was measured to be SPF 15. It is indicated that the mussel mucin product has the function of protecting the skin from ultraviolet rays.
  • Example 3 Application of mussel mucin gel medical device in radiation therapy protection.
  • Example 4 Application of mussel mucin foam cosmetic in computer radiation protection.
  • Control product preparation A commercially available carboxymethyl cellulose substrate gel cosmetic product, which does not contain mussel mucin.
  • the melanin value of the observed site was measured before use, and the melanin value of the observed site was measured on the 90th day after use.
  • the melanin increased by 5.7% ⁇ 1.2% after 90 days; using the cheek of one side of the control product, the melanin increased by 12.4% ⁇ 0.9% after 90 days.
  • the increase in melanin in the control group was significantly higher than in the experimental group.
  • the mussel mucin product has the function of protecting the skin from radiation.
  • Example 5 Application of mussel mucin liquid cosmetics in the protection of environmental pollutants.
  • the melanin value of the observed site was measured before use, and the melanin value of the observed site was measured on the 180th day after use.
  • the melanin increased by 3.9% ⁇ 2.7% after 180 days; using the cheek of one side of the control product, the melanin increased by 18.4% ⁇ 1.5% after 180 days.
  • the increase in melanin in the control group was significantly higher than in the experimental group.
  • the mussel mucin product has the function of protecting the skin from environmental pollution.
  • Example 6 Application of mussel mucin liquid medical device in skin burn protection.
  • a mussel mucin solution having a concentration of 10.0 mg/ml was prepared and diluted with physiological saline to prepare a mussel mucin liquid medical device, wherein the mussel mucin concentration was 2.5 mg/ml.
  • the dry weight/wet weight of the test site of the above-mentioned mussel mucin liquid medical device applied to the right side of 20 rats was 33.4% ⁇ 3.8% on average; 20 rats were coated with physiological saline on the left side as a control.
  • the dry weight/wet weight of the part was 17.9% ⁇ 2.2% on average.
  • the edema of the test site coated with the mussel mucin liquid medical device was significantly lighter than that of the control group. It is indicated that mussel mucin products have the effect of protecting the skin from high temperature liquids.
  • Example 7 Application of mussel mucin hydrogel drug in the protection of skin frostbite.
  • Control product preparation a gel containing no mussel mucin as a substrate for cationic guar gum.
  • the dry weight/wet weight of the test site of the above mussel mucin hydrogel drug coated on the right side of 20 rats was 33.4% ⁇ 3.8% on average; 20 rats were coated on the left side without mussel mucin
  • the dry weight/wet weight of the test site of the cationic guar gel as a control was 17.9% ⁇ 2.2% on average.
  • the edema of the test site coated with the mussel mucin product was significantly lighter than that of the control group. It is indicated that mussel mucin products have the effect of protecting the skin from high temperature liquids.
  • Example 8 Application of mussel mucoid hair gel cosmetic (essential product) in seawater protection.
  • the mucin hair care essence that is, the hair gel cosmetic, wherein the mussel mucin concentration is 1.2 mg/ml.
  • the scaly skin of the test group volunteers using the above mussel mucin hair care essence was intact, and there was no difference from the blank control of the test group.
  • the control group volunteers who did not use mussel mucin hair care essence were incomplete in the scaly rind, which was different from the control blank.
  • mussel mucin products have the effect of protecting the skin from seawater erosion.
  • Example 9 Application of mussel mucoprotein hair care liquid cosmetic (essential aquatic product) in allergy protection caused by hair dyeing.
  • mussel mucin solution Take 100ml of mussel mucin solution with a concentration of 10.0mg/ml, add 3g of glutathione, and dilute with purified water to prepare mussel mucoprotein hair care essence water, which is a hair care cosmetic.
  • the mussel mucin concentration is 3.9. Mg/ml.
  • Example 10 Application of Mussel Muscle Hair Care Gel Cosmetic (Essence Product) in allergy protection caused by perm.
  • mussel mucin solution Take 50ml of mussel mucin solution with a concentration of 10.0mg/ml, dilute with purified water, and add 10g of gelatin to prepare mussel mucoprotein hair care essence gel, which is a hair care cosmetic.
  • the mussel mucin concentration is 2.0mg/ g.

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Abstract

本发明公开了贻贝粘蛋白或其制剂在防护皮肤和皮肤附属器官的应用。具体地公开了贻贝粘蛋白或其制剂在防护皮肤和头发等皮肤附属器官免受紫外线损伤等的应用；防护皮肤和皮肤附属器官免受电磁、射线、电脑辐射等放射线损伤等的应用；防护皮肤和头发等皮肤附属器官免受高温物体等的应用；防护皮肤和头发等皮肤附属器官免受低温物体等的应用；防护皮肤和头发等皮肤附属器官免受海水损伤等的应用；防护皮肤和头发等皮肤附属器官免受环境污染等损伤的应用；防护皮肤和头发等皮肤附属器官免受烫、染试剂等损伤的应用。

Description

贻贝粘蛋白用于皮肤和皮肤附属器官的防护 技术领域
本发明大体涉及日化产品、化妆品、医疗器械、药品、消毒产品、保健品等领域，并且涉及一种贻贝粘蛋白或其制剂在防护皮肤和皮肤附属器官中的应用。
背景技术
皮肤作为人体的第一道生理防线和最大的器官，时刻参与机体的功能活动，维持着机体和自然环境的对立统一，机体的任何异常情况也可以在皮肤表面反映出来。皮肤由表皮、真皮和皮下组织构成，并含有众多附属器官如毛发、汗腺、皮脂腺、指甲、趾甲以及血管、***、神经和肌肉等。皮肤具备着近乎完美的生理保护功能，如屏障作用、感觉作用、体温调节作用、吸收作用、分泌和***作用等，在维护机体的健康方面起十分重要的作用。
皮肤及其附属器官易于受到由紫外线、放射线(如电磁、射线、电脑辐射等)、高温物体所致烫伤、低温物体所致冻伤、海水、环境污染以及烫染试剂等的损伤。
日光中的中波紫外线(UVB)在强烈照射时会引起皮肤和皮肤附属器官的红、肿、灼热、灼痛、水肿、皮肤脱屑等损伤；引起头发发黄、分叉、易断、干燥、缺乏弹性，并且一旦损伤无法修复。
包括放射性治疗在内的电离辐射等射线对恶性肿瘤有一定治疗作用，但会出现一定程度的皮肤反应，例如：红斑、烧灼和刺痒感、脱屑、充血、水肿、水泡、渗出、糜烂、溃疡、皮肤萎缩，毛细血管扩张、淋巴引流障碍、深棕色斑点、色素沉着等。
烫伤是因皮肤接触高于体温的物体而造成的皮肤红肿、水疱、脱皮、组织坏死、溃烂、溃疡等问题。
冻伤是由于寒冷潮湿作用引起的皮肤和皮肤附属器官的局部损伤，表现为冰凉、苍白、坚硬、感觉麻木或丧失、红肿充血、热、痒、灼痛、水疱、水肿、剧痛、组织坏死。
海水中的盐分和游泳池中的氯气都会损伤头皮使之出现毛孔粗大等炎症反应，头发也会变得干枯。
空气中的主要污染物有二氧化硫、氮氧化物、粒子状污染物、酸雨。二氧化硫、一氧化二氮(N2O)、一氧化氮(NO)、二氧化氮(NO2)、三氧化二氮(N2O3)等含氮污染物对人体的皮肤有强烈刺激性。颗粒状污染物数量大且成分复杂，它本身可以是有毒物质或是其它污染物的运载体，易引发皮肤炎症，出现毛孔粗大、毛囊红肿、色素沉着等问题。
染发剂具有改变头发颜色的作用，含有金属、氧化物、对苯二胺等有毒有害物质，造成头皮过敏、红肿、瘙痒等。烫发试剂中的巯基乙酸类物质有害于头发、头皮和毛囊，致使鳞片遭到破坏，易使头发发黄、发脆、脱落、失去光泽和弹性。
贻贝粘蛋白(Mussel adhesive protein，MAP)，也称作贻贝足丝蛋白(Mytilus edulis foot protein，Mefp)，是海洋贝类紫贻贝(Mytilus edulis Linnaeus)、厚壳贻贝(Mytilus coruscus)、 翡翠贻贝(Perna viridis)等分泌的一种特殊的蛋白质。贻贝通常成群地附着在海岸边的礁石上或者轮船的底部，有在近海耐受波浪冲击的能力。实际上贻贝几乎可以极其牢固地附着在任何材料的基底上，如金属、木材、玻璃等。贻贝具有上述特性的主要原因是其足丝腺内可生成并储存这种特殊的粘蛋白，贻贝通过足丝释放粘蛋白到岩石一类的固体表面上，形成抗水的结合，从而将自己固定。
目前从贻贝中鉴定得到11种粘蛋白亚类，包括mefp1、mefp-2、mefp-3、mefp-4、mefp-5、mefp-6、胶原蛋白pre-COL-P、pre-COL-D、pre-COL-NG、足丝基质蛋白PTMP和DTMP(朱曜曜等，海洋科学进展，2014，32(4)：560-568)。贻贝粘蛋白具有2个结构特点：(1)含有赖氨酸，使蛋白带有高载量正电荷；(2)含3，4二羟基苯丙氨酸(DOPA，多巴)。人体的细胞和组织带有负电荷。贻贝粘蛋白通过自身正电荷与人体的细胞和组织负电荷之间的静电相互作用与细胞和组织紧密结合，发挥防护和治疗的作用。此外，多巴氧化生成邻位二醌，可以和未被氧化的多巴相互交联形成膜或是网状支架，促使蛋白质更加紧密、稳固地附着在人体表面，起到保护作用。贻贝粘蛋白是大分子蛋白质，在人体内完全降解的时间约为3-10天，其附着于细胞组织的能力优异，使贻贝粘蛋白可以稳固于局部，持续发挥作用。
虽然贻贝粘蛋白具有以上特点，但目前其产品应用领域非常有限。商品化的贻贝粘蛋白产品有美国BD Biosciences公司的Cell-Tak，韩国Kollodis的MAP Trix和瑞典Biopolymer的Hydrogel。这些产品或者是以贻贝粘蛋白溶液状态直接使用，或者是以冻干粉制剂保存而在使用前溶解，它们的主要应用领域限于微观的细胞粘附和组织粘合剂。也有报道贻贝粘蛋白用于胎膜修复、作为抗海水腐蚀涂层、心脏载药支架等应用。
发明内容
本发明的目的是提供一种贻贝粘蛋白产品。
在本文中使用的贻贝粘蛋白是指从贻贝科(Mytilidae)双壳类软体动物中的紫贻贝(Mytilus edulis Linnaeus)、厚壳贻贝(Mytilus coruscus)或翡翠贻贝(Perna viridis)等海洋贻贝中纯化获得的、目前已知的贻贝粘蛋白11个亚类：mefp1、mefp-2、mefp-3、mefp-4、mefp-5、mefp-6、胶原蛋白pre-COL-P、pre-COL-D、pre-COL-NG、足丝基质蛋白PTMP和DTMP中的一种或几种的混合物。在本文中使用的贻贝粘蛋白在水溶液中的酸碱度可以是pH1.0-7.0，特别是可以在pH 3.0-6.5的范围内以使其治疗效果更佳。
在本文中使用的贻贝粘蛋白可以采用以下制备方法获得，例如中国专利号ZL200710179491.0的一种使用混合吸附色谱分离纯化贻贝粘蛋白的方法，中国专利号ZL200710179492.5的一种使用羧甲基离子交换色谱纯化贻贝粘蛋白的方法，中国专利号ZL200910087567.6的一种使用盐析和透析分离纯化贻贝粘蛋白的方法等。
在本文中使用的贻贝粘蛋白可以是溶液或冻干粉形式，特别是贻贝粘蛋白在产品中的浓度可以是0.1-15.0mg/ml，当浓度过低时，贻贝粘蛋白的功效不大，当浓度过高时，可因正电荷载量过高引起细胞毒性，从而不利于发挥防护作用。
在本文中使用的贻贝粘蛋白也可以与辅料结合而制备成液体剂。示例性的贻贝粘蛋白液 体剂是将贻贝粘蛋白溶液母液或冻干粉溶解或稀释至一定浓度或pH值制得，溶解或稀释用溶液可以是水、生理盐水、磷酸盐溶液、醋酸盐溶液、硼酸盐溶液等等。最终产品中贻贝粘蛋白的酸碱度可以是pH 1.0-7.0，特别是可以在pH 3.0-6.5的范围内治疗效果更佳。
在本文中使用的贻贝粘蛋白也可以与辅料结合而制备成凝胶剂。示例性的贻贝粘蛋白凝胶剂是将贻贝粘蛋白溶液或冻干粉与凝胶基质材料混合制得，所述凝胶基质材料可以是选自纤维素衍生物、卡波姆和海藻酸盐、西黄蓍胶、明胶、果胶、卡拉胶、结冷胶、淀粉、黄原胶、阳离子瓜尔胶、琼脂、非纤维素多糖、乙烯聚合物、丙烯酸树脂、聚乙烯醇或聚羧乙烯中之一或其任意组合。
在本文中使用的贻贝粘蛋白也可以与辅料结合而制备成凝露。示例性的贻贝粘蛋白凝露是由贻贝粘蛋白溶液或冻干粉与凝露基质混合制得，所述凝露基质可以包括纤维素衍生物、甘油、非纤维素多糖、丙二醇中之一或其任意组合。
在本文中使用的贻贝粘蛋白也可以与辅料结合而制备成糊剂。示例性的贻贝粘蛋白糊剂是由贻贝粘蛋白与糊剂基质材料混合制得，所述糊剂基质材料可以包括甘油、凡士林、石蜡等。
本领域技术人员可以根据不同病期的临床适应症和皮损特点来选择使用上述剂型或其他合适的剂型。
在本文中使用的贻贝粘蛋白还可以与基质材料结合形成用于皮肤表面的敷料和敷贴。示例性的贻贝粘蛋白敷贴中的液体可以是贻贝粘蛋白溶液，或是其与纤维素衍生物、卡波姆和海藻酸盐、西黄蓍胶、明胶、果胶、卡拉胶、结冷胶、淀粉、黄原胶、阳离子瓜尔胶、琼脂、非纤维素多糖、乙烯聚合物、丙烯酸树脂、聚乙烯醇或聚羧乙烯、明胶、鱼胶、果胶、海藻酸盐、甘油、凡士林、石蜡、聚乙二醇、维生素、谷胱甘肽中的一种或多种组合。上述贻贝粘蛋白溶液或组合浸泡在基质材料如纱布、无纺布、蚕丝纸上，所述敷贴可以是例如创口贴、面膜、眼膜、手膜、脚膜等。
在本文中使用的贻贝粘蛋白还可以制成泡沫剂。示例性的贻贝粘蛋白泡沫剂是由贻贝粘蛋白溶液或冻干粉与发泡剂基质混合制得，所述发泡剂基质材料可以包括羟丙甲基纤维素、明胶、聚乙二醇十二烷基硫酸钠、脂肪醇聚氧乙烯醚磺酸钠、玉米麸质粉、丙烯酰胺之一或其任意组合。特别有益的是，泡沫剂在消泡过程中释放压力，更有利于贻贝粘蛋白在患处的附着、渗透和起效。同时泡沫剂消泡时间长，延长了作用时间，使贻贝粘蛋白起效更加显著。
可以向上述包括贻贝粘蛋白的各种制剂中进一步添加甘油、聚乙二醇、维生素、谷胱甘肽等本领域公知的可增加其保湿、抗氧化等能力的成分以进一步提高其保湿、抗氧化的能力。
可以向上述包括贻贝粘蛋白的各种制剂中进一步添加芦荟、葡萄籽、茶叶、人参、蜗牛等提取物，氨基酸、胶原蛋白、透明质酸等成分，增加其营养皮肤和皮肤附属器官的能力，增加滑润程度。
以上所有制剂都可采用本领域周知的方法制备，详细的操作步骤可以参照例如《制剂学》。
在本文中使用的贻贝粘蛋白可以作为主要原料，采用药学上可接受的载体制备药品。所 述药品可以是液体剂、凝胶剂、凝露、糊剂、敷贴、泡沫剂。所述药品可用于体外使用，特别是可用于皮肤外用。
在本文中使用的贻贝粘蛋白可以作为主要原料，制备医疗器械。本文中使用的术语医疗器械是指直接或间接用于人体的材料及其他类似或相关的物品。所述医疗器械可以是液体剂、凝胶剂、凝露、糊剂、敷贴、泡沫剂。所述医疗器械可用于体外使用，特别是可用于皮肤外用。
在本文中使用的贻贝粘蛋白可以作为主要原料，采用化妆品领域可接受的辅料制备化妆品。所述化妆品可以是液体剂、凝胶剂、凝露、糊剂、敷贴、泡沫剂。所述化妆品可用于体外使用，特别是可用于皮肤外用。
在本文中使用的贻贝粘蛋白可以作为主要原料，采用消毒产品领域可接受的辅料制备消毒产品。本文中使用的术语消毒产品是指用化学、物理、生物的方法杀灭或者消除环境中病原微生物的消毒剂、消毒器械、卫生用品和一次性使用的医疗用品。所述消毒产品可以是液体剂、凝胶剂、凝露、糊剂、敷贴、泡沫剂。所述消毒产品可用于体外使用，特别是可用于皮肤外用。
在本文中使用的贻贝粘蛋白可以作为主要原料，采用保健品或食品领域可接受的辅料制备保健品或食品。所述保健品或食品可以是液体剂、凝胶剂、凝露、糊剂、敷贴、泡沫剂。所述保健品或食品可用于体外使用或食用，特别是可用于皮肤外用。
在本文中使用的贻贝粘蛋白可以作为主要原料，采用日化产品领域可接受的辅料制备日化产品。本文中使用的术语日化产品是指平日常用的科技化学制品，包括洗发水、沐浴露等。所述日化产品可以是液体剂、凝胶剂、凝露、糊剂、敷贴、泡沫剂。所述日化产品可用于体外使用，特别是可用于皮肤外用。
特别是，可以由本发明的贻贝粘蛋白制备护发和美发产品，用于头皮和头发等附属器官等的防护。
本发明的另一个目的是提供贻贝粘蛋白产品在防护皮肤和皮肤附属器官中的应用。
特别是，本发明提供了贻贝粘蛋白或其制剂在防护皮肤和头发等皮肤附属器官免受紫外线损伤等中的应用。
特别是，本发明提供了贻贝粘蛋白或其制剂在防护皮肤和皮肤附属器官免受放射线(电磁、射线、电脑辐射等)损伤等中的应用。
特别是，本发明提供了贻贝粘蛋白或其制剂在防护皮肤和头发等皮肤附属器官免受高温物体等引发的烫伤中的应用。
特别是，本发明提供了贻贝粘蛋白或其制剂在防护皮肤和头发等皮肤附属器官免受低温物体等引发的冻伤中的应用。
特别是，本发明提供了贻贝粘蛋白或其制剂在防护皮肤和头发等皮肤附属器官免受海水损伤中的应用；在防护皮肤和头发等皮肤附属器官免受环境污染等损伤中的应用。
特别是，本发明提供了贻贝粘蛋白或其制剂在防护皮肤和头发等皮肤附属器官免受烫、染试剂等损伤中的应用。
具体实施方式
本发明的实施方式包括：
1、贻贝粘蛋白在防护皮肤和皮肤附属器官损伤中的应用。
2、根据实施方式1的贻贝粘蛋白应用，其中所述贻贝粘蛋白可以是来自亚类：mefp1、mefp-2、mefp-3、mefp-4、mefp-5、mefp-6、胶原蛋白pre-COL-P、pre-COL-D、pre-COL-NG、足丝基质蛋白PTMP和DTMP中的一种或几种的混合物。
3、根据实施方式1的贻贝粘蛋白应用，其中所述贻贝粘蛋白浓度可以是0.1-15.0mg/ml。
4、根据实施方式1的贻贝粘蛋白应用，其中所述贻贝粘蛋白可以是以液体剂、凝胶剂、凝露、糊剂、敷贴或泡沫剂使用。
5、根据实施方式1的贻贝粘蛋白应用，其中最终产品中的贻贝粘蛋白可以是在pH 1.0-7.0的范围内，特别是可以在pH 3.0-6.5的范围内。
6、根据实施方式1-4中任一项的贻贝粘蛋白应用，其中所述防护皮肤和皮肤附属器官可以包括防护皮肤和头发等皮肤附属器官免受紫外线、放射线、高温物体、低温物体、海水、环境污染、烫和染试剂的损伤。
7、贻贝粘蛋白作为活性成分在用于防护皮肤和皮肤附属器官的组合物中的应用，其中所述组合物可以是以液体剂、凝胶剂、凝露、糊剂、敷贴或泡沫剂使用。
8、根据实施方式7的贻贝粘蛋白应用，其中所述组合物可以是皮肤外用。
9、贻贝粘蛋白作为活性成分在用于防护皮肤和皮肤附属器官的药品中的应用。
10、贻贝粘蛋白作为活性成分在用于防护皮肤和皮肤附属器官的化妆品中的应用。
11、贻贝粘蛋白作为活性成分在用于防护皮肤和皮肤附属器官的消毒产品中的应用。
12、贻贝粘蛋白作为活性成分在用于防护皮肤和皮肤附属器官的保健品或食品中的应用。
13、贻贝粘蛋白作为活性成分在用于防护皮肤和皮肤附属器官的日化产品中的应用。
14、贻贝粘蛋白作为活性成分在用于防护皮肤和皮肤附属器官的护发和美发产品中的应用。
15、根据实施方式14的贻贝粘蛋白应用，其中所述护发和美发产品可以包括护发素、护发精华水、护发凝胶等。
16、根据实施方式7-14中任一项的贻贝粘蛋白应用，其中所述贻贝粘蛋白是以泡沫剂使用。
下面将结合具体实施例对本发明作进一步说明。需要指出的是，由本发明中的贻贝粘蛋白或贻贝粘蛋白的各种制剂形成的药品、医疗器械、化妆品、消毒产品、保健品或食品、日化产品在施用于受试者后，都可以应用于上文所述的适应症并展现出上文所述的功能，在本发明范围内的所有剂型均已测试，下文中，仅仅是为说明，只在实施例中描述了其中一少部分，然而不应将其理解为对本发明的限制。
除非特殊说明，否则本发明中所使用的试剂都是市售可购买的。
实施例1：贻贝粘蛋白水凝胶化妆品在紫外线防护(晒伤防护)中的应用。
取明胶10g，加入100ml去离子水，90℃温浴30min至基质完全溶解，另取浓度为20.0mg/ml的贻贝粘蛋白溶液60ml，边搅拌边加入到凝胶基质中，用生理盐水调整浓度，混合均匀后形成贻贝粘蛋白水凝胶化妆品，其中贻贝粘蛋白浓度为10mg/g。
收集10位志愿者，既往无光感性疾病史，近期内未使用影响光感性的药物，受试部位的皮肤无色素沉着、炎症、瘢痕、色素痣、多毛等。采用日光模拟器氙弧灯作为光源，连续产生波长为290nm-400nm的紫外线，照射受试者后背至受试部位皮肤出现红斑。按2mg样品/cm2的用量称取上述贻贝粘蛋白水凝胶化妆品并涂布，涂布面积为40cm2，测试经贻贝粘蛋白产品防护之后和未经防护的皮肤的最低的红斑照射剂量。
用SPF＝最低红斑剂量(用防晒用品后)/最低红斑剂量(用防晒用品前)公式计算防晒系数SPF值。
测得本实施例的贻贝粘蛋白水凝胶化妆品的防晒系数是SPF 25。表明贻贝粘蛋白产品具有防护皮肤免受紫外线侵害的作用。
实施例2：贻贝粘蛋白液体化妆品在紫外线防护(晒伤防护)中的应用。
取浓度为10.0mg/ml的贻贝粘蛋白溶液50ml，用生理盐水稀释，添加维生素C粉末10mg，制备成贻贝粘蛋白液体化妆品，其中贻贝粘蛋白浓度为2mg/ml。
收集12位志愿者，既往无光感性疾病史，近期内未使用影响光感性的药物，受试部位的皮肤无色素沉着、炎症、瘢痕、色素痣、多毛等。采用日光模拟器氙弧灯作为光源，连续产生波长为290nm-400nm的紫外线，照射受试者后背至受试部位皮肤出现红斑。按2mg样品/cm2的用量称取上述贻贝粘蛋白水凝胶化妆品并喷布，喷涂面积30-40cm2，测试经贻贝粘蛋白产品放护之后和未经防护的皮肤的最低的红斑照射剂量。
用SPF＝最低红斑剂量(用防晒用品后)/最低红斑剂量(用防晒用品前)公式计算防晒系数SPF值。
测得本实施例的贻贝粘蛋白化液体化妆品的防晒系数是SPF 15。表明贻贝粘蛋白产品具有防护皮肤免受紫外线侵害的作用。
实施例3：贻贝粘蛋白凝胶医疗器械在放射性治疗防护中的应用。
取羟丙基甲基纤维素20g，加入100ml去离子水，90℃温浴30min至基质完全溶解，加入丙二醇5g，丙三醇8g制备凝胶基质，另取浓度为20.0mg/ml的贻贝粘蛋白溶液100ml，用生理盐水稀释，边搅拌边加入到凝胶基质中，混合均匀后形成贻贝粘蛋白凝胶医疗器械，其中贻贝粘蛋白浓度为8mg/g。对照产品制备：市售的卡波姆凝胶医疗器械，不含有贻贝粘蛋白。
收集10例正在进行腰椎放射性治疗的志愿者，5例在放疗前半小时涂布上述贻贝粘蛋白凝胶医疗器械，按照5mg样品/cm2的用量称取样品，然后放射治疗。5例涂布未添加贻贝粘 蛋白的对照医疗器械放射性治疗。5例涂布贻贝粘蛋白凝胶医疗器械的患者在放疗后未出现皮肤损伤。5位涂布卡波姆凝胶的患者有2例出现I度烧灼伤，表现为红斑。表明本发明的贻贝粘蛋白产品具有防护皮肤免受放射线侵害的作用。
实施例4：贻贝粘蛋白泡沫剂化妆品在电脑辐射防护中的应用。
取羟丙基甲基纤维素10g，加生理盐水，室温下制备成0.5v/v％的泡沫剂，稳定过夜；另取浓度为20.0mg/ml的贻贝粘蛋白溶液10ml，用生理盐水稀释至2.5mg/ml。灌装到泡沫喷瓶中使用。对照组产品制备：市售的羧甲基纤维素基材凝胶化妆品产品，不含有贻贝粘蛋白。
收集20位志愿者，年龄20-40岁之间，职业特点：每日使用阴极射线显像管电脑屏幕时间不少于6小时。20名志愿者一侧脸颊使用试验组贻贝粘蛋白泡沫剂化妆品，一侧脸颊使用对照组产品，选择面颊部1％体表面积作为观察区域，每日接触电脑之间、之中和之后共使用3次，连续使用90天。
使用前测定观察部位的黑色素值，使用后第90天测定观察部位的黑色素值。使用贻贝粘蛋白泡沫剂化妆品一侧脸颊，90天后黑色素增加比例5.7％±1.2％；使用对照产品一侧脸颊，90天后黑色素增加比例12.4％±0.9％。对照组黑色素增加水平显著高于试验组。
表明贻贝粘蛋白产品具有防护皮肤免受射线侵害的作用。
实施例5：贻贝粘蛋白液体化妆品在环境污染物防护中的应用。
取浓度为5.0mg/ml的贻贝粘蛋白溶液100ml，用生理盐水稀释，添加谷胱甘肽3mg，制备成贻贝粘蛋白液体化妆品，其中贻贝粘蛋白浓度为0.5mg/ml。
收集30位志愿者，年龄20-40岁之间，生活在北京、河北区域，存在颗粒物污染(PM2.5颗粒)。30名志愿者一侧脸颊使用试验组贻贝粘蛋白液体化妆品，一侧脸颊使用市售清洁型化妆水作为对照组，选择面颊部1％体表面积作为观察区域，每日早晚面部清洁后各使用2次，连续使用180天。
使用前测定观察部位的黑色素值，使用后第180天测定观察部位的黑色素值。使用贻贝粘蛋白液体化妆品一侧脸颊，180天后黑色素增加比例3.9％±2.7％；使用对照产品一侧脸颊，180天后黑色素增加比例18.4％±1.5％。对照组黑色素增加水平显著高于试验组。
表明贻贝粘蛋白产品具有防护皮肤免受环境污染的作用。
实施例6：贻贝粘蛋白液体医疗器械在皮肤烫伤防护中的应用。
取浓度为10.0mg/ml的贻贝粘蛋白溶液50ml，用生理盐水稀释，制备成贻贝粘蛋白液体医疗器械，其中贻贝粘蛋白浓度为2.5mg/ml。
取20只200g的白色大鼠，背部右侧部位喷涂贻贝粘蛋白液体医疗器械，1次/min，同一部位连续喷涂5次；背部左侧部位喷涂生理盐水作为对照，1次/min，同一部位连续喷涂5次。大鼠麻醉后，固定于鼠板上，将90℃水罐装到直径2cm塑料瓶中，在受试部位持续放 置30sec，形成烫伤。
24h后剪下烫伤皮肤秤量湿重，然后放入60℃烤箱烤干48h，称量干重。将烘干之后的皮肤质量除以烘干之前的皮肤质量，得到的比值用于评估烫伤造成的水肿程度，比值越高表明水肿越轻。
20只大鼠右侧涂布上述贻贝粘蛋白液体医疗器械的受试部位的干重/湿重，平均为33.4％±3.8％；20只大鼠左侧涂布生理盐水作为对照的受试部位的干重/湿重，平均为17.9％±2.2％。
涂布了贻贝粘蛋白液体医疗器械的受试部位水肿情况明显比对照组轻。表明贻贝粘蛋白产品具有防护皮肤免受高温液体侵害的作用。
实施例7：贻贝粘蛋白水凝胶药品在皮肤冻伤防护中的应用。
取阳离子瓜尔胶20g，加生理盐水，室温下制备成水凝胶，稳定过夜；另取浓度为20.0mg/ml的贻贝粘蛋白溶液5ml，边搅拌边添加至水凝胶中，凝胶中贻贝粘蛋白的最终浓度为1.8mg/ml。对照产品制备：阳离子瓜尔胶为基材的不含贻贝粘蛋白的凝胶。
取20只200g的白色大鼠，背部右侧部位涂布上述贻贝粘蛋白水凝胶药品，6mg/cm2；背部左侧部位涂布对照产品，6mg/cm2。大鼠麻醉后，固定于鼠板上，将-20℃水罐装到直径2cm塑料瓶中，在受试部位持续放置5min，形成烫伤。
24h后剪下烫伤皮肤秤量湿重，然后放入60℃烤箱烤干48h，称量干重。烘干之后到皮肤质量除以烘干之前到皮肤质量，得到的比值用于评估烫伤造成的水肿程度，比值越高表明水肿越轻。
20只大鼠右侧涂布上述贻贝粘蛋白水凝胶药品的受试部位的干重/湿重，平均为33.4％±3.8％；20只大鼠左侧涂布不含贻贝粘蛋白的阳离子瓜尔胶凝胶作为对照的受试部位的干重/湿重，平均为17.9％±2.2％。
涂布了贻贝粘蛋白产品的受试部位水肿情况明显比对照组轻。表明贻贝粘蛋白产品具有防护皮肤免受高温液体侵害的作用。
实施例8：贻贝粘蛋白护发凝胶化妆品(精华露产品)在海水防护中的应用。
取浓度为3.0mg/ml的贻贝粘蛋白溶液200ml，用纯化水稀释，添加羧甲基纤维素2g，甘油3ml，芦荟提取物溶液10ml，充分搅拌均匀，过夜12h使制剂稳定，制备贻贝粘蛋白护发精华露，即护发凝胶化妆品，其中贻贝粘蛋白浓度为1.2mg/ml。
收集20位志愿者，年龄20-30岁之间。试验组10位志愿者，取毛发在显微镜下观察鳞状上皮作为试验组空白对照，游泳前在干燥的毛发表面均匀喷涂上述贻贝粘蛋白护发精华露，单一面积下反复喷涂3次，待完全干燥后，入海水，持续在水中3小时，出水后取毛发，在显微镜下观察鳞状外皮的形态。对照组10位志愿者，取毛发在显微镜下观察鳞状上皮作为对照组空白对照，直接入海水，持续在水中3小时，出水后取毛发，在显微镜下观察鳞状外皮的形态。
使用上述贻贝粘蛋白护发精华露的试验组志愿者毛发鳞状外皮完整，与试验组空白对照无差异。未使用贻贝粘蛋白护发精华露的对照组志愿者毛发鳞状外皮不完整，与对照组空白对照有差异。
表明贻贝粘蛋白产品具有防护皮肤免受海水侵蚀的作用。
实施例9：贻贝粘蛋白护发液体化妆品(精华水产品)在染发引发的过敏防护中的应用。
取浓度为10.0mg/ml的贻贝粘蛋白溶液100ml，添加谷胱甘肽3g，用纯化水稀释，制备贻贝粘蛋白护发精华水，即护发化妆品，其中贻贝粘蛋白浓度为3.9mg/ml。
收集10位志愿者，年龄40-50岁之间。志愿者属于敏感皮肤，过往既有染发过程中发生严重过敏的病史。染发之前，在干燥的头皮上，采用可持续供给护发精华水的压力梳子，涂布上述贻贝粘蛋白精华水，反复涂布3次，待完全干燥。在毛发表面均匀喷涂上述贻贝粘蛋白护发精华水，单一面积下反复喷涂3次，待完全干燥后，开始染发。染发完成后观察24h是否出现过敏现象。
染发后24h观察，10位志愿者中有8位未出现任何过敏现象，2位出现头皮轻度瘙痒症状，瘙痒VAS评分3分左右。表明贻贝粘蛋白护发化妆品具有预防染发引发过敏的作用。
实施例10：贻贝粘蛋白护发凝胶化妆品(精华露产品)在烫发引发的过敏防护中的应用。
取浓度为10.0mg/ml的贻贝粘蛋白溶液50ml，用纯化水稀释，添加明胶10g，制备贻贝粘蛋白护发精华凝胶，即护发化妆品，其中贻贝粘蛋白浓度为2.0mg/g。
收集6位志愿者，年龄30-50岁之间。志愿者属于敏感皮肤，过往既有烫发过程中发生严重过敏的病史。烫发之前，在干燥的头皮上，涂布上述贻贝粘蛋白精华凝胶，待完全干燥，开始烫发。烫发完成后观察24h是否出现过敏现象。
烫发后24h观察，6位志愿者均未出现任何过敏现象。表明贻贝粘蛋白产品具有预防烫发引发过敏的作用。

Claims (15)

  1. 贻贝粘蛋白在防护皮肤和皮肤附属器官中的应用。
  2. 根据权利要求1的贻贝粘蛋白应用，其中所述贻贝粘蛋白是来自亚类：mefp1、mefp-2、mefp-3、mefp-4、mefp-5、mefp-6、胶原蛋白pre-COL-P、pre-COL-D、pre-COL-NG、足丝基质蛋白PTMP和DTMP中的一种或几种的混合物。
  3. 根据权利要求1的贻贝粘蛋白应用，其中所述贻贝粘蛋白浓度为0.1-15.0mg/ml。
  4. 根据权利要求1的贻贝粘蛋白应用，其中所述贻贝粘蛋白是以液体剂、凝胶剂、凝露、糊剂、敷贴或泡沫剂使用。
  5. 根据权利要求1的贻贝粘蛋白应用，其中最终产品中的贻贝粘蛋白是在pH 1.0-7.0的范围内，特别是在pH 3.0-6.5的范围内。
  6. 根据权利要求14中任一项的贻贝粘蛋白应用，其中所述防护皮肤和皮肤附属器官包括防护皮肤和头发等皮肤附属器官免受紫外线、放射线、高温物体、低温物体、海水、环境污染、烫和染试剂的损伤。
  7. 贻贝粘蛋白作为活性成分在用于防护皮肤和皮肤附属器官的组合物中的应用，其中所述组合物是以液体剂、凝胶剂、凝露、糊剂、敷贴或泡沫剂使用。
  8. 根据权利要求7的贻贝粘蛋白应用，其中所述组合物是皮肤外用。
  9. 贻贝粘蛋白作为活性成分在用于防护皮肤和皮肤附属器官的药品中的应用。
  10. 贻贝粘蛋白作为活性成分在用于防护皮肤和皮肤附属器官的化妆品中的应用。
  11. 贻贝粘蛋白作为活性成分在用于防护皮肤和皮肤附属器官的消毒产品中的应用。
  12. 贻贝粘蛋白作为活性成分在用于防护皮肤和皮肤附属器官的保健品或食品中的应用。
  13. 贻贝粘蛋白作为活性成分在用于防护皮肤和皮肤附属器官的日化产品中的应用。
  14. 贻贝粘蛋白作为活性成分在用于防护皮肤和皮肤附属器官的护发和美发产品中的应用。
  15. 根据权利要求9-14中任一项的贻贝粘蛋白应用，其中所述贻贝粘蛋白是以泡沫剂使用。
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CN107137338A (zh) * 2017-07-07 2017-09-08 苏州蓬拓生物医学科技有限公司 一种基于表面涂层原理的毛发染色方法
CN114057833A (zh) * 2021-11-02 2022-02-18 浙江工业大学 基于减少黑色素沉淀功效的贻贝多肽、多肽粉及制备方法和应用
CN114057833B (zh) * 2021-11-02 2024-03-19 浙江工业大学 基于减少黑色素沉淀功效的贻贝多肽、多肽粉及制备方法和应用
CN116617109A (zh) * 2023-06-05 2023-08-22 广州植境生物科技有限公司 一种贻贝粘蛋白头皮修护组合物及其制备方法和应用
CN116617109B (zh) * 2023-06-05 2024-02-20 广州植境生物科技有限公司 一种贻贝粘蛋白头皮修护组合物及其制备方法和应用
CN117398511A (zh) * 2023-10-18 2024-01-16 珠海市雅莎医疗器械有限公司 激光、光子术后修复的贻贝粘蛋白修护敷料及其制备方法
CN117398511B (zh) * 2023-10-18 2024-04-12 珠海市雅莎医疗器械有限公司 激光、光子术后修复的贻贝粘蛋白修护敷料及其制备方法
CN117982710A (zh) * 2024-02-18 2024-05-07 韩伟东 一种重组双蛋白医用敷料的制备方法

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