WO2016206442A1 - Method for producing beer-brewing syrup based on corn direct method - Google Patents

Method for producing beer-brewing syrup based on corn direct method Download PDF

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Publication number
WO2016206442A1
WO2016206442A1 PCT/CN2016/078612 CN2016078612W WO2016206442A1 WO 2016206442 A1 WO2016206442 A1 WO 2016206442A1 CN 2016078612 W CN2016078612 W CN 2016078612W WO 2016206442 A1 WO2016206442 A1 WO 2016206442A1
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Prior art keywords
water
corn
sugar
resin
solution
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PCT/CN2016/078612
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French (fr)
Chinese (zh)
Inventor
马俊辉
马容珊
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镇赉县绿禾有机食品有限公司
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Publication of WO2016206442A1 publication Critical patent/WO2016206442A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00—Preparation of wort
    • C12C7/04—Preparation or treatment of the mash

Definitions

  • the invention belongs to the technical field of food fermentation, and particularly relates to a beer brewing syrup production method based on the direct corn method.
  • Corn starch is the purest starch raw material for brewers. The reason why it is not widely used is that its price is more expensive than corn flour or brewed rice. However, the price of corn starch has been close to the price of rice in recent years. The brewer offers a good choice. The content of protein, fat and polyphenol in corn starch is very low. The use of corn starch as an auxiliary material can prolong the shelf life of beer, improve the flavor stability of beer and reduce the color of beer. Corn starch can be completely converted to soluble matter, so it does not cause filtration problems. The market prospects for corn starch applications depend to a large extent on their relative prices.
  • the existing starch-based raw material for the production of beer-making syrup has the following problems: the corn starch process requires long-time immersion and water washing, and all the free protein precipitated is washed away, so that even if protease is added during saccharification, it is in the sugar liquid. There is no protein and it cannot be converted to nitrogen. Therefore, nitrogen sources cannot be provided for beer.
  • the technical scheme adopted by the invention is a beer brewing syrup production method based on the direct corn method, which is specifically implemented according to the following steps:
  • Step 1 peeling and degerming the corn to prepare corn flour
  • Step 2 Performing a slurry treatment on the corn flour
  • Step 3 liquefying the corn flour after the pulping
  • Step 4 filtering and slaging the liquefied liquid
  • Step 5 saccharifying the filtered filtrate to obtain a saccharification solution
  • Step 6 Decolorize and filter the saccharification solution
  • Step 7 Perform ion exchange treatment on the decolorized saccharified solution
  • Step 8 Perform evaporation treatment on the ion exchanged saccharification solution
  • Step 9 The finished product is packaged to prepare a finished syrup for beer brewing.
  • the corn flour prepared in the step 1 is specifically: fineness 40 mesh all passed, 60 mesh 80% pass; germ and skin yield ⁇ 18%; corn flour yield ⁇ 80%; earth stone, weed ⁇ 1%; Corn flour fat content ⁇ 0.1%; corn flour water ⁇ 15%.
  • the step of adjusting the corn flour in the step 2 is specifically: adding a certain amount of food grade water in the mixing tank; adding steam to the temperature between 56 ° C and 60 ° C; adding CaCl 2 according to the mass concentration of 0.1% - 0.5% ; After the mixing of the mixing tank is started, the corn powder is continuously added to the tank from the material silo, and the mass percentage of the corn flour and the food grade water is 38%-40%, and the pH of the corn flour is adjusted between 5.8-6.0, and then added.
  • ⁇ -amylase wherein the amount of ⁇ -amylase added is 6 ⁇ / g, and the mixture is uniform.
  • step 3 the liquefaction of the corn flour after slurry adjustment is as follows: adjusting the steam supply pressure of the liquefier to 6 kg/cm 2 , checking that the pipeline valve is in the working position, starting the slurry pump; opening the steam inlet of the jet liquefier Adjust the steam intake while opening the feed valve to control the feed flow rate; the injection temperature is always maintained at 106 °C-108 °C; the liquefied liquid after the injection enters the multiple liquefied liquid holding tanks in sequence, and stir for 20 minutes.
  • the DE value is measured, and the DE value is between 18% and 20%.
  • the reaction is completed, and the liquefied liquid is obtained.
  • the concentration of the liquefied liquid is between 20% and 25%, and the pH of the liquefied liquid is 5.2 to 5.3.
  • step 4 the liquefaction liquid is filtered and slag is specifically as follows: the oil pump is started to be compacted, filtered, and the feed pump is started, and the feed pressure is 3 kg/cm 2 ; after the filtration is completed, the mass percentage of the residual sugar is washed by hot water. 3%, the obtained filtrate has a light yellow color, a transmittance of ⁇ 90%, and a zein residue of ⁇ 60%.
  • step 5 the filtered filtrate is saccharified to obtain a saccharification liquid.
  • the saccharification tank is washed and steam autoclaved for 20 minutes, wherein the pressure is 4 kg/cm 2 ; and the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger.
  • the amount is 2-4 ⁇ /g, 0.5L/M 3 saccharification solution and 0.1L/M 3 saccharification solution; stir for 2 minutes at intervals of 30 minutes; DE value is measured every 4 hours, and saccharification is terminated when DE ⁇ 50
  • the saccharification solution has maltose ⁇ 50%, maltotriose ⁇ 23%, and glucose ⁇ 10%.
  • step 6 the saccharification solution is decolorized and filtered.
  • the activated carbon is added to the decolorization tank, and the mass ratio of the saccharification solution and the activated carbon is 100:2.
  • the filter is driven into the filter to start the filtration.
  • the feed to the pre-discharge tank is started until the filtration and the residual sugar is ⁇ 3%, the filtration is finished, and the obtained filtrate is colorless or yellowish, the transmittance is >98%, and the color value is ⁇ 0.05.
  • the loss rate is ⁇ 2%.
  • the ion exchange treatment of the decolorized saccharified solution in step 7 is specifically as follows:
  • Step 7.1 preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;
  • Step 7.2 new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; Repeatedly soaking twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, after being washed with water to meet the requirements;
  • Step 7.3 Preparation of non-ionized water: using 5 times the volume of the tap water of the resin to flow through the anion and cation exchange columns from top to bottom, and measuring the conductivity of the water by using a conductivity meter for a plurality of times, and after being qualified, it is put into the ion-free water tank for use; When the conductivity of the effluent rises to 50 mn/cm 2 , that is, when the resistance is lower than 20,000 ohms, the production of ion-free water is stopped, and regeneration is awaited; the cation and the cation resin are respectively washed with 5% alkali and acid, and then used in the ion-free water.
  • Step 7.4 Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the cation off the column from top to bottom, and the water is 3% in the water.
  • step 8 the ion-exchanged saccharification solution is subjected to evaporation treatment, specifically: adjusting the steam supply pressure of the evaporator to 5-6 kg/cm 2 or more, and starting the feed pump, so that the saccharification liquid enters the first effect, the second effect, and the third.
  • the vacuum pump is started to vacuum, and the vacuum degree of each effect is sequentially reached.
  • the steam valve is slowly opened for evaporation.
  • the sugar meter is often used to sample the concentration from the sampling port, and the concentration is required to be sent to the finished product tank.
  • the end of evaporation when the sugar is topped with sugar to ⁇ 3%, stop the water top sugar, open the vacuum valve, break the vacuum, turn off the steam, and cycle for 3 minutes, then stop sequentially.
  • the beneficial effects of the invention are: using corn instead of starch to produce beer brewing special syrup can make tons of sugar The cost is reduced by 300-500 yuan.
  • the use of corn instead of starch to produce beer-making syrup can increase the nitrogen source for beer fermentation, and improve the beer flavor and improve the quality of beer.
  • the invention provides a beer brewing syrup production method based on the direct corn method, comprising the following steps:
  • Step 1 The corn is peeled and de-emerged to prepare corn flour; the peeling and de-emergence process is a conventional technique, and the obtained corn flour has the following indexes: fineness of 40 meshes all passed, 60 meshes of 80% pass; germ and skin yield ⁇ 18%; corn flour yield ⁇ 80%; earth and stone, weeds ⁇ 1%; corn flour fat content ⁇ 0.1%; corn flour moisture ⁇ 15%.
  • Step 2 mixing the corn flour; adding a certain amount of food grade water in the slurry tank; adding steam to the temperature between 56 ° C and 60 ° C; adding CaCl 2 according to the mass concentration of 0.1% - 0.5%; After the mixing tank is stirred, the corn powder is continuously added to the tank from the material silo, the mass percentage of corn flour and food grade water is 38%-40%, and after adjusting the pH between 5.8-6.0, the ⁇ -amylase is added, The amount of addition was 6 ⁇ / g, and the mixture was evenly stirred.
  • Step 3 Liquefy the corn flour after slurry adjustment; adjust the steam supply pressure of the liquefier to 6kg/cm 2 ; check that the pipeline valve is in the working position of the material, start the slurry pump; turn on the steam inlet of the jet liquefier, adjust Steam intake, at the same time open the feed valve, control the feed flow; the injection temperature is always maintained at 106 ° C -108 ° C; the liquefied liquid after the injection into the liquefied liquid holding tank once in sequence, after stirring for 20 minutes, sampling The DE value is measured, and the DE value is between 18% and 20%. The reaction is completed, and the liquefied liquid is obtained. The concentration of the liquefied liquid is between 20% and 25%, and the pH of the liquefied liquid is 5.2 to 5.3.
  • Step 4 Filter and remove the slag from the liquefied liquid; start the oil pump to compact, filter, start the pump, and feed the pressure 3kg/cm 2 ; after the filtration, rinse with hot water and the percentage of residual sugar is ⁇ 3%
  • the obtained filtrate has a light yellow color, a transmittance of ⁇ 90%, and a zein residue of ⁇ 60%.
  • Step 5 saccharifying the filtered filtrate to obtain a saccharification solution: the saccharification tank is washed and steam autoclaved for 20 minutes, wherein the pressure is 4 kg/cm 2 ; the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger, and then adjusted.
  • the temperature is 52 ° C -55 ° C, the pH is adjusted between 5.2-5.3; the fungal enzyme, pullulanase and neutral protease are added in sequence and stirred, wherein the addition amount of fungal enzyme, pullulanase and neutral protease respectively It is 2-4 ⁇ / g, 0.5 L / M 3 saccharification solution and 0.1 L / M 3 saccharification solution; stirring for 2 minutes at intervals of 30 minutes; DE value is measured every 4 hours, saccharification is terminated when DE ⁇ 50, and the obtained saccharification Maltose in the liquid ⁇ 50%, maltotriose ⁇ 23% and glucose ⁇ 10%.
  • Step 6 Decolorize and filter the saccharification solution; add activated carbon to the decolorization tank, wherein the mass ratio of the saccharification solution and the activated carbon is 100:2, and after maintaining the temperature at 85 ° C for 30 minutes, the filter is driven into the filter to start the filtration. After reaching 98% or more, the feed to the pre-discharge tank is started until the filtration and the residual sugar is ⁇ 3%, the filtration is finished, and the obtained filtrate is colorless or yellowish, the transmittance is >98%, and the color value is ⁇ 0.05. The loss rate is ⁇ 2%.
  • Step 7 Perform ion exchange treatment on the decolorized saccharified solution
  • Step 7.1 preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;
  • Step 7.2 new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; the cation and anion resin are respectively soaked twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, and after being washed with water, the requirements are ready for use. ;
  • Step 7.3 Preparation of non-ionized water: use tap water of 5 times the volume of the resin to flow through the anion and cation exchange columns from top to bottom, and measure the conductivity of the water with a conductivity meter several times. After being qualified (in accordance with national regulations), Into the ion-free water tank for standby; when the conductivity of the effluent rises to 50mn/cm 2 , that is, when the resistance is lower than 20,000 ohms, the production of non-ionized water is stopped, waiting for regeneration; the yin and yang resins are soaked with 5% alkali and acid respectively.
  • Step 7.4 Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the cation off the column from top to bottom, and the water is 3% in the water.
  • Step 8 Evaporating the ion-exchanged saccharification solution: adjusting the steam supply pressure of the evaporator to 5-6 kg/cm 2 or more, and starting the feed pump, so that the saccharification liquid sequentially enters the first-, second-, and third-effect evaporation.
  • the vacuum pump is started to evacuate, and the vacuum degree of each effect is sequentially reached.
  • the steam valve is slowly opened for evaporation, and the concentration is sampled from the sampling port by the sugar meter, and the concentration is required to feed the finished product tank after evaporation;
  • the sugar is topped with sugar to ⁇ 3%, stop the water top sugar, open the vacuum valve, break the vacuum, turn off the steam, and cycle for 3 minutes, then stop sequentially;
  • Step 9 The finished product is packaged to prepare a finished syrup for beer brewing.
  • the sensory index of the brewed syrup prepared by the above preparation process is:
  • Appearance It is a viscous transparent liquid with no visible impurities
  • Aroma has the normal smell of sugar for beer brewing
  • the hygienic indicators of this beer brewing syrup are as follows:
  • the technical achievement is based on the production of starch syrup, maltose syrup and fructose syrup on the basis of starch for many years.
  • corn flour in production
  • the process technology saves the link of sugar production after corn starch production, and achieves the purpose of reducing the cost of sugar production and improving economic benefits.
  • it provides high-quality brewing raw materials for the beer industry, saving a lot of grain for beer brewing, and reducing the production of beer production enterprises.
  • the beer brewing syrup produced by the direct method of corn is rich in nitrogen source, which plays an important role in improving beer quality and improving beer flavor.
  • the corn starch process requires long-term soaking and water washing, and all the free proteins precipitated are washed away, so that even if the protease is added during the saccharification, the protein cannot be converted into nitrogen because there is no protein in the sugar liquid.
  • the corn flour is obtained by machine pulverization. When the corn is liquefied, there is a free protein precipitated. When the saccharification is added to the protease, nitrogen is produced. With the nitrogen source, the beer is better. The flavor, improve the quality of beer, bring better economic benefits to beer manufacturers.
  • amylase amylase, fungal enzyme, pullulanase, protease, lipase
  • PH value has a great influence on the activity of the enzyme, and the pH value should be strictly controlled in the production, each enzyme
  • the preparation has its own pH value under the best use condition, neither too low nor too high, otherwise it will affect the activity of the enzyme preparation and the efficiency of enzyme reaction. Therefore, in actual production, the PH value of the feed liquid should be strictly controlled.
  • the pH value should be adjusted, the pH should be adjusted first and then the enzyme should be added.
  • the temperature of the enzyme preparation should be selected at the optimum temperature. The temperature should not be too low, but the enzyme reaction should be slow. If the temperature is too high, the enzyme denaturation will lose activity.
  • a beer brewing syrup production method based on the direct corn method comprising the following steps:
  • Step 1 The corn is peeled and de-emerged to prepare corn flour; the peeling and de-emergence process is a conventional technique, and the obtained corn flour has the following indexes: fineness of 40 meshes all passed, 60 meshes of 80% pass; germ and skin yield ⁇ 18%; corn flour yield ⁇ 80%; earth and stone, weeds ⁇ 1%; corn flour fat content ⁇ 0.1%; corn flour moisture ⁇ 15%.
  • Step 2 Mixing the corn flour; adding a certain amount of food grade water in the pulping tank; adding steam to the temperature of 58 ° C; adding CaCl 2 according to the mass concentration of 0.3%; and stirring the mixing tank
  • the corn powder is added to the tank continuously from the material warehouse.
  • the mass percentage of corn flour and food grade water is 39%.
  • the pH is adjusted between 5.8 and 6.0, the ⁇ -amylase is added, and the addition amount is 6 ⁇ /g, and the mixture is evenly stirred. .
  • Step 3 Liquefy the corn flour after slurry adjustment; adjust the steam supply pressure of the liquefier to 6kg/cm 2 ; check that the pipeline valve is in the working position of the material, start the slurry pump; turn on the steam inlet of the jet liquefier, adjust Steam intake, at the same time open the feed valve, control the feed flow; the injection temperature is always maintained at 107 ° C; the liquefied liquid after injection into the liquefied liquid insulation tank once in sequence, after stirring for 20 minutes, sample and measure the DE value The DE value is between 18% and 20%, indicating that the reaction is completed, and the liquefied liquid is obtained.
  • the concentration of the liquefied liquid is between 23%, and the pH of the liquefied liquid is 5.2.
  • Step 4 Filter and remove the slag from the liquefied liquid; start the oil pump to compact, filter, start the pump, and feed the pressure 3kg/cm 2 ; after the filtration, rinse with hot water and the percentage of residual sugar is ⁇ 3%
  • the obtained filtrate has a light yellow color, a transmittance of ⁇ 90%, and a zein residue of ⁇ 60%.
  • Step 5 saccharifying the filtered filtrate to obtain a saccharification solution: the saccharification tank is washed and steam autoclaved for 20 minutes, wherein the pressure is 4 kg/cm 2 ; the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger, and then adjusted.
  • the temperature is 54 ° C, the pH is adjusted between 5.2-5.3; the fungal enzyme, pullulanase and neutral protease are added in sequence and stirred, wherein the addition amount of fungal enzyme, pullulanase and neutral protease is 3 ⁇ / g, 0.5L/M 3 saccharification solution and 0.1L/M 3 saccharification solution; stir for 2 minutes at intervals of 30 minutes; sample DE value every 4 hours, terminate saccharification when DE ⁇ 50, get maltose ⁇ 50 in saccharification solution %, maltotriose ⁇ 23% and glucose ⁇ 10%.
  • Step 6 Decolorize and filter the saccharification solution; add activated carbon to the decolorization tank, wherein the mass ratio of the saccharification solution and the activated carbon is 100:2, and after maintaining the temperature at 85 ° C for 30 minutes, the filter is driven into the filter to start the filtration. After reaching 98% or more, the feed to the pre-discharge tank is started until the filtration and residual sugar are ⁇ 3%. At the end, the obtained filtrate was specifically colorless or yellowish in color, transmittance was >98%, color value was ⁇ 0.05, and loss rate was ⁇ 2%.
  • Step 7 Perform ion exchange treatment on the decolorized saccharified solution
  • Step 7.1 preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;
  • Step 7.2 new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; the cation and anion resin are respectively soaked twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, and after being washed with water, the requirements are ready for use. ;
  • Step 7.3 Preparation of non-ionized water: use 5 times of the volume of the tap water to flow through the anion and cation exchange columns from top to bottom, and measure the conductivity of the water with a conductivity meter several times. After being qualified (in accordance with national regulations), Into the ion-free water tank for standby; when the conductivity of the effluent rises to 50mn/cm 2 , that is, when the resistance is lower than 20,000 ohms, the production of non-ionized water is stopped, waiting for regeneration; the yin and yang resins are soaked with 5% alkali and acid respectively.
  • Step 7.4 Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the cation off the column from top to bottom, and the water is 3% in the water.
  • Step 8 Evaporating the ion-exchanged saccharification solution: adjusting the steam supply pressure of the evaporator to 5-6 kg/cm 2 or more, and starting the feed pump, so that the saccharification liquid sequentially enters the first-, second-, and third-effect evaporation.
  • the vacuum pump is started to evacuate, and the vacuum degree of each effect is sequentially reached.
  • the steam valve is slowly opened for evaporation, and the concentration is sampled from the sampling port by the sugar meter, and the concentration is required to feed the finished product tank after evaporation;
  • the sugar is topped with sugar to ⁇ 3%, stop the water top sugar, open the vacuum valve, break the vacuum, turn off the steam, and cycle for 3 minutes, then stop sequentially;
  • Step 9 The finished product is packaged to prepare a finished syrup for beer brewing.
  • a beer brewing syrup production method based on the direct corn method comprising the following steps:
  • Step 1 The corn is peeled and de-emerged to prepare corn flour; the peeling and de-emergence process is a conventional technique, and the obtained corn flour has the following indexes: fineness of 40 meshes all passed, 60 meshes of 80% pass; germ and skin yield ⁇ 18%; corn flour yield ⁇ 80%; earth and stone, weeds ⁇ 1%; corn flour fat content ⁇ 0.1%; corn flour moisture ⁇ 15%.
  • Step 2 mixing the corn flour; adding a certain amount of food grade water in the mixing tank; adding steam to the temperature of 56 ° C; adding CaCl 2 according to the mass concentration of 0.1%; after stirring the mixing tank,
  • the material warehouse is continuously added with corn flour into the pool.
  • the mass percentage of corn flour and food grade water is 38%.
  • the ⁇ -amylase is added. The amount of addition was 6 ⁇ / g, and the mixture was evenly stirred.
  • Step 3 Liquefy the corn flour after slurry adjustment; adjust the steam supply pressure of the liquefier to 6kg/cm 2 ; check that the pipeline valve is in the working position of the material, start the slurry pump; turn on the steam inlet of the jet liquefier, adjust Steam intake, at the same time open the feed valve, control the feed flow; the injection temperature is always maintained at 106 ° C; the liquefied liquid after injection into the liquefied liquid insulation tank once in sequence, after stirring for 20 minutes, sample and measure the DE value The DE value is between 18% and 20%, indicating that the reaction is completed, and the liquefied liquid is obtained.
  • the concentration of the liquefied liquid is between 20% and 25%, and the pH of the liquefied liquid is between 5.2 and 5.3.
  • Step 4 Filter and remove the slag from the liquefied liquid; start the oil pump to compact, filter, start the pump, and feed the pressure 3kg/cm 2 ; after the filtration, rinse with hot water and the percentage of residual sugar is ⁇ 3%
  • the obtained filtrate has a light yellow color, a transmittance of ⁇ 90%, and a zein residue of ⁇ 60%.
  • Step 5 saccharifying the filtered filtrate to obtain a saccharification solution: the saccharification tank is washed and steam autoclaved for 20 minutes, wherein the pressure is 4 kg/cm 2 ; the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger, and then adjusted.
  • the temperature is 52 ° C, the pH is adjusted between 5.2-5.3; the fungal enzyme, pullulanase and neutral protease are added in sequence and stirred, wherein the addition amount of fungal enzyme, pullulanase and neutral protease is 2 ⁇ / g, 0.5L/M 3 saccharification solution and 0.1L/M 3 saccharification solution; stir for 2 minutes at intervals of 30 minutes; sample DE value every 4 hours, terminate saccharification when DE ⁇ 50, get maltose ⁇ 50 in saccharification solution %, maltotriose ⁇ 23% and glucose ⁇ 10%.
  • Step 6 Decolorize and filter the saccharification solution; add activated carbon to the decolorization tank, wherein the mass ratio of the saccharification solution and the activated carbon is 100:2, and after maintaining the temperature at 85 ° C for 30 minutes, the filter is driven into the filter to start the filtration. After reaching 98% or more, the feed to the pre-discharge tank is started until the filtration and the residual sugar is ⁇ 3%, the filtration is finished, and the obtained filtrate is colorless or yellowish, the transmittance is >98%, and the color value is ⁇ 0.05. The loss rate is ⁇ 2%.
  • Step 7 Perform ion exchange treatment on the decolorized saccharified solution
  • Step 7.1 preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;
  • Step 7.2 new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; Repeatedly soaking twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, after being washed with water to meet the requirements;
  • Step 7.3 Preparation of non-ionized water: use 5 times of the volume of the tap water to flow through the anion and cation exchange columns from top to bottom, and measure the conductivity of the water with a conductivity meter several times. After being qualified (in accordance with national regulations), Into the ion-free water tank for standby; when the conductivity of the effluent rises to 50mn/cm 2 , that is, when the resistance is lower than 20,000 ohms, the production of non-ionized water is stopped, waiting for regeneration; the yin and yang resins are soaked with 5% alkali and acid respectively.
  • Step 7.4 Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the cation off the column from top to bottom, and the water is 3% in the water.
  • Step 8 Evaporating the ion-exchanged saccharification solution: adjusting the steam supply pressure of the evaporator to 5-6 kg/cm 2 or more, and starting the feed pump, so that the saccharification liquid sequentially enters the first-, second-, and third-effect evaporation.
  • the vacuum pump is started to evacuate, and the vacuum degree of each effect is sequentially reached.
  • the steam valve is slowly opened for evaporation, and the concentration is sampled from the sampling port by the sugar meter, and the concentration is required to feed the finished product tank after evaporation;
  • the sugar is topped with sugar to ⁇ 3%, stop the water top sugar, open the vacuum valve, break the vacuum, turn off the steam, and cycle for 3 minutes, then stop sequentially;
  • Step 9 The finished product is packaged to prepare a finished syrup for beer brewing.
  • a beer brewing syrup production method based on the direct corn method comprising the following steps:
  • Step 1 The corn is peeled and de-emerged to prepare corn flour; the peeling and de-emergence process is a conventional technique, and the obtained corn flour has the following indexes: fineness of 40 meshes all passed, 60 meshes of 80% pass; germ and skin yield ⁇ 18%; corn flour yield ⁇ 80%; earth and stone, weeds ⁇ 1%; corn flour fat content ⁇ 0.1%; corn flour moisture ⁇ 15%.
  • Step 2 Mixing the corn flour; adding a certain amount of food grade water in the pulping tank; adding steam to the temperature of 60 ° C; adding CaCl 2 according to the mass concentration of 0.5%; and stirring the mixing tank
  • the corn powder is added to the tank continuously from the material warehouse.
  • the mass percentage of corn flour and food grade water is 40%.
  • the ⁇ -amylase is added, and the addition amount is 6 ⁇ /g, and the mixture is evenly stirred. .
  • Step 3 Liquefy the corn flour after slurry adjustment; adjust the steam supply pressure of the liquefier to 6kg/cm 2 ; check that the pipeline valve is in the working position of the material, start the slurry pump; turn on the steam inlet of the jet liquefier, adjust Steam intake, at the same time open the feed valve, control the feed flow; the injection temperature is always maintained at 108 ° C; the liquefied liquid after injection into the liquefied liquid insulation tank once in sequence, after stirring for 20 minutes, sample and measure the DE value The DE value is between 18% and 20%, indicating that the reaction is completed, and the liquefied liquid is obtained.
  • the concentration of the liquefied liquid is between 20% and 25%, and the pH of the liquefied liquid is between 5.2 and 5.3.
  • Step 4 Filter and remove the slag from the liquefied liquid; start the oil pump to compact, filter, start the pump, and feed the pressure 3kg/cm 2 ; after the filtration, rinse with hot water and the percentage of residual sugar is ⁇ 3%
  • the obtained filtrate has a light yellow color, a transmittance of ⁇ 90%, and a zein residue of ⁇ 60%.
  • Step 5 saccharifying the filtered filtrate to obtain a saccharification solution: the saccharification tank is washed and steam autoclaved for 20 minutes, wherein the pressure is 4 kg/cm 2 ; the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger, and then adjusted.
  • the temperature is 54 ° C, the pH is adjusted between 5.2-5.3; the fungal enzyme, pullulanase and neutral protease are added in sequence and stirred, wherein the addition amount of fungal enzyme, pullulanase and neutral protease is 4 ⁇ / g, 0.5L/M 3 saccharification solution and 0.1L/M 3 saccharification solution; stir for 2 minutes at intervals of 30 minutes; sample DE value every 4 hours, terminate saccharification when DE ⁇ 50, get maltose ⁇ 50 in saccharification solution %, maltotriose ⁇ 23% and glucose ⁇ 10%.
  • Step 6 Decolorize and filter the saccharification solution; add activated carbon to the decolorization tank, wherein the mass ratio of the saccharification solution and the activated carbon is 100:2, and after maintaining the temperature at 85 ° C for 30 minutes, the filter is driven into the filter to start the filtration. After reaching 98% or more, the feed to the pre-discharge tank is started until the filtration and the residual sugar is ⁇ 3%, the filtration is finished, and the obtained filtrate is colorless or yellowish, the transmittance is >98%, and the color value is ⁇ 0.05. The loss rate is ⁇ 2%.
  • Step 7 Perform ion exchange treatment on the decolorized saccharified solution
  • Step 7.1 preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;
  • Step 7.2 new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; the cation and anion resin are respectively soaked twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, and after being washed with water, the requirements are ready for use. ;
  • Step 7.3 Preparation of non-ionized water: use 5 times of the volume of the tap water to flow through the anion and cation exchange columns from top to bottom, and measure the conductivity of the water with a conductivity meter several times. After being qualified (in accordance with national regulations), Into the ion-free water tank for standby; when the conductivity of the effluent rises to 50mn/cm 2 , that is, when the resistance is lower than 20,000 ohms, the production of non-ionized water is stopped, waiting for regeneration; the yin and yang resins are soaked with 5% alkali and acid respectively.
  • Step 7.4 Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the yin from the top to the bottom, and the water is 3% in the water.
  • Step 8 Evaporating the ion-exchanged saccharification solution: adjusting the steam supply pressure of the evaporator to 5-6 kg/cm 2 or more, and starting the feed pump, so that the saccharification liquid sequentially enters the first-, second-, and third-effect evaporation.
  • the vacuum pump is started to evacuate, and the vacuum degree of each effect is sequentially reached.
  • the steam valve is slowly opened for evaporation, and the concentration is sampled from the sampling port by the sugar meter, and the concentration is required to feed the finished product tank after evaporation;
  • the sugar is topped with sugar to ⁇ 3%, stop the water top sugar, open the vacuum valve, break the vacuum, turn off the steam, and cycle for 3 minutes, then stop sequentially;
  • Step 9 The finished product is packaged to prepare a finished syrup for beer brewing.

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Abstract

A method for producing beer-brewing syrup based on a corn direct method is specifically implemented according to the following steps: performing decrustation and degermination on corn, and preparing the corn into corn flour; performing syrup mixing treatment on the corn flour; liquefying the syrup-mixed corn flour; filtering liquefied liquid to remove residue; saccharifying the filtered filtrate to obtain saccharified liquid; performing decolored filtration on the saccharified liquid; performing ion exchange treatment on the decolored saccharified liquid; performing evaporation treatment on the saccharified liquid having undergone ion exchange; and performing finished product packaging, and preparing to obtain the finished beer-brewing syrup. Producing beer-brewing syrup by using corn instead of starch can increase a nitrogen source for beer fermentation, so as to improve beer flavor and quality, and can reduce the cost of sugar by 300-500 yuan per ton.

Description

一种基于玉米直接法的啤酒酿造糖浆生产方法Beer brewing syrup production method based on corn direct method 技术领域Technical field
本发明属于食品发酵技术领域，具体涉及一种基于玉米直接法的啤酒酿造糖浆生产方法。The invention belongs to the technical field of food fermentation, and particularly relates to a beer brewing syrup production method based on the direct corn method.
背景技术Background technique
玉米淀粉对于酿造师来说是一种最纯的淀粉原料，它没有得到广泛使用的原因主要是其价格比玉米粉或酿造大米昂贵，不过近几年来玉米淀粉的价格已接近大米的价格，为酿造师提供了良好的选择余地。玉米淀粉的蛋白质、脂肪、多酚含量很低，使用玉米淀粉作为辅料能延长啤酒的保质期、提高啤酒的风味稳定性、降低啤酒的色度。玉米淀粉可全部转化为可溶性物质，所以不会引起过滤问题。玉米淀粉应用的市场前景在很大程度上取决于其相对价格。现有的用淀粉做原料生产啤酒酿造糖浆存在以下问题：玉米制淀粉过程中需长时间浸泡和水洗，将析出的游离态蛋白全部都洗掉了，这样在糖化时即使加入蛋白酶，由于糖液中没有蛋白而不能转化为氮。因此，不能为啤酒提供氮源。Corn starch is the purest starch raw material for brewers. The reason why it is not widely used is that its price is more expensive than corn flour or brewed rice. However, the price of corn starch has been close to the price of rice in recent years. The brewer offers a good choice. The content of protein, fat and polyphenol in corn starch is very low. The use of corn starch as an auxiliary material can prolong the shelf life of beer, improve the flavor stability of beer and reduce the color of beer. Corn starch can be completely converted to soluble matter, so it does not cause filtration problems. The market prospects for corn starch applications depend to a large extent on their relative prices. The existing starch-based raw material for the production of beer-making syrup has the following problems: the corn starch process requires long-time immersion and water washing, and all the free protein precipitated is washed away, so that even if protease is added during saccharification, it is in the sugar liquid. There is no protein and it cannot be converted to nitrogen. Therefore, nitrogen sources cannot be provided for beer.
发明内容Summary of the invention
本发明的目的是提供一种基于玉米直接法的啤酒酿造糖浆生产方法，解决了现有技术中存在的啤酒酿造糖浆啤酒的风味差的问题。It is an object of the present invention to provide a beer brewing syrup production method based on the direct corn method, which solves the problem of poor flavor of beer brewing syrup beer existing in the prior art.
本发明所采用的技术方案是，一种基于玉米直接法的啤酒酿造糖浆生产方法，具体按照以下步骤实施： The technical scheme adopted by the invention is a beer brewing syrup production method based on the direct corn method, which is specifically implemented according to the following steps:
步骤1、对玉米进行脱皮脱胚，制备成玉米粉；Step 1. peeling and degerming the corn to prepare corn flour;
步骤2、对玉米粉进行调浆处理；Step 2. Performing a slurry treatment on the corn flour;
步骤3、对调浆后的玉米粉进行液化；Step 3: liquefying the corn flour after the pulping;
步骤4、对液化液进行过滤除渣；Step 4: filtering and slaging the liquefied liquid;
步骤5、对过滤后的滤液进行糖化，得到糖化液；Step 5, saccharifying the filtered filtrate to obtain a saccharification solution;
步骤6、对糖化液进行脱色过滤；Step 6. Decolorize and filter the saccharification solution;
步骤7、对脱色后的糖化液进行离子交换处理；Step 7. Perform ion exchange treatment on the decolorized saccharified solution;
步骤8、对离子交换后的糖化液进行蒸发处理；Step 8. Perform evaporation treatment on the ion exchanged saccharification solution;
步骤9、成品包装，制备得到成品啤酒酿造用糖浆。Step 9. The finished product is packaged to prepare a finished syrup for beer brewing.
本发明的特点还在于，The invention is also characterized in that
步骤1中制备得到的玉米粉具体为：细度40目全部通过，60目80％通过；胚芽、皮得率<18％；玉米粉收得率≥80％；土石、杂草≤1％；玉米粉脂肪含量<0.1％；玉米粉水分<15％。The corn flour prepared in the step 1 is specifically: fineness 40 mesh all passed, 60 mesh 80% pass; germ and skin yield <18%; corn flour yield ≥ 80%; earth stone, weed ≤ 1%; Corn flour fat content <0.1%; corn flour water <15%.
步骤2中对玉米粉进行调浆处理具体为：在调浆罐内加入一定量的食品级用水；加蒸汽升温到56℃-60℃之间；按质量浓度为0.1％-0.5％加入CaCl2；开动调浆罐的搅拌后，由物料仓连续加玉米粉入池，玉米粉与食品级用水的质量百分比为38％-40％，调整玉米粉浆的PH在5.8-6.0之间后，加入ɑ-淀粉酶，其中，ɑ-淀粉酶的添加量为6υ/g，搅拌均匀。The step of adjusting the corn flour in the step 2 is specifically: adding a certain amount of food grade water in the mixing tank; adding steam to the temperature between 56 ° C and 60 ° C; adding CaCl 2 according to the mass concentration of 0.1% - 0.5% ; After the mixing of the mixing tank is started, the corn powder is continuously added to the tank from the material silo, and the mass percentage of the corn flour and the food grade water is 38%-40%, and the pH of the corn flour is adjusted between 5.8-6.0, and then added. Ɑ-amylase, wherein the amount of ɑ-amylase added is 6 υ / g, and the mixture is uniform.
步骤3中对调浆后的玉米粉进行液化具体为：调整液化器的供汽压力为6kg/cm2，检查管路阀门处于打料工作位置，开动调浆打料泵；开启喷射液化器蒸汽进口，调整蒸汽进量，同时开启进料阀门，控制进料流量；喷射温度始终保持在106℃-108℃；经喷射后的液化液按先后顺序一次进入多个液化液保温罐中，搅拌20分钟后取样测DE值，DE值在18％-20％之间，标志 反应结束，得到液化液，液化液的浓度在20％-25％之间，液化液的pH值为5.2-5.3。In step 3, the liquefaction of the corn flour after slurry adjustment is as follows: adjusting the steam supply pressure of the liquefier to 6 kg/cm 2 , checking that the pipeline valve is in the working position, starting the slurry pump; opening the steam inlet of the jet liquefier Adjust the steam intake while opening the feed valve to control the feed flow rate; the injection temperature is always maintained at 106 °C-108 °C; the liquefied liquid after the injection enters the multiple liquefied liquid holding tanks in sequence, and stir for 20 minutes. After the sample, the DE value is measured, and the DE value is between 18% and 20%. The reaction is completed, and the liquefied liquid is obtained. The concentration of the liquefied liquid is between 20% and 25%, and the pH of the liquefied liquid is 5.2 to 5.3.
步骤4中对液化液进行过滤除渣具体为：启动油泵压紧，过滤,启动打料泵，进料压力3kg/cm2；过滤结束后，用热水冲洗过滤并到残糖的质量百分比<3％，得到的滤液色泽浅黄色，透光度为≥90％，玉米蛋白渣含水≤60％。In step 4, the liquefaction liquid is filtered and slag is specifically as follows: the oil pump is started to be compacted, filtered, and the feed pump is started, and the feed pressure is 3 kg/cm 2 ; after the filtration is completed, the mass percentage of the residual sugar is washed by hot water. 3%, the obtained filtrate has a light yellow color, a transmittance of ≥90%, and a zein residue of ≤60%.
步骤5中对过滤后的滤液进行糖化得到糖化液具体为：将糖化缸洗净后蒸汽高压灭菌20分钟，其中，压力为4kg/cm2；液化液经板式换热器泵入糖化罐后，调整温度为52℃-55℃，调pH在5.2-5.3之间；依次加入真菌酶、普鲁兰酶和中性蛋白酶并搅拌，其中，真菌酶、普鲁兰酶和中性蛋白酶的添加量分别为2-4υ/g、0.5L/M3糖化液和0.1L/M3糖化液；间隔30分钟搅拌2分钟；每隔4个小时取样测DE值，DE≥50时终止糖化，得到的糖化液中麦芽糖≥50％、麦芽三糖≤23％以及葡萄糖≤10％。In step 5, the filtered filtrate is saccharified to obtain a saccharification liquid. Specifically, the saccharification tank is washed and steam autoclaved for 20 minutes, wherein the pressure is 4 kg/cm 2 ; and the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger. Adjust the temperature to 52 ° C -55 ° C, adjust the pH between 5.2-5.3; add fungal enzyme, pullulanase and neutral protease in sequence and stir, among which, add fungal enzyme, pullulanase and neutral protease The amount is 2-4υ/g, 0.5L/M 3 saccharification solution and 0.1L/M 3 saccharification solution; stir for 2 minutes at intervals of 30 minutes; DE value is measured every 4 hours, and saccharification is terminated when DE ≥ 50 The saccharification solution has maltose ≥ 50%, maltotriose ≤ 23%, and glucose ≤ 10%.
步骤6中对糖化液进行脱色过滤具体为：往脱色罐内加入活性炭，糖化液和活性炭的质量比为100:2，维持85℃30分钟后，打入过滤机内开始过滤，在透光率达到98％以上后开始向离交前贮罐进料，直至滤并残糖<3％时过滤结束，得到的滤液具体为色泽无色或微黄色，透光度>98％，色值<0.05，损失率<2％。In step 6, the saccharification solution is decolorized and filtered. Specifically, the activated carbon is added to the decolorization tank, and the mass ratio of the saccharification solution and the activated carbon is 100:2. After maintaining the temperature at 85 ° C for 30 minutes, the filter is driven into the filter to start the filtration. After reaching 98% or more, the feed to the pre-discharge tank is started until the filtration and the residual sugar is <3%, the filtration is finished, and the obtained filtrate is colorless or yellowish, the transmittance is >98%, and the color value is <0.05. The loss rate is <2%.
步骤7中对脱色后的糖化液进行离子交换处理具体为：The ion exchange treatment of the decolorized saccharified solution in step 7 is specifically as follows:
步骤7.1、再生处理液的配制，制备质量浓度为10％的氯化钠溶液，制备质量浓度为5％的盐酸溶液和氢氧化钠溶液；Step 7.1, preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;
步骤7.2、新树脂处理，将阴、阳离子交换树脂分别从离交柱视镜孔中装入柱内；从立交柱上部进水，洗树脂到澄清透明；用10％的氯化钠溶液从柱上浸泡离子交换树脂到20小时后，用水洗到无色透明；阳、阴树脂分别 用上述盐酸溶液和氢氧化钠溶液反复两次浸泡，每次8小时，在用水串洗达到要求后待用；Step 7.2, new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; Repeatedly soaking twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, after being washed with water to meet the requirements;
步骤7.3、无离子水的制备：用5倍于树脂体积的自来水自上而下流经阴、阳离子交换柱，多次用电导仪测水电导率，待合格后，打入无离子水缸备用；当出水电导率上升到50mn/cm2时，即电阻低于2万欧姆时停止制作无离子水，等待再生；阴、阳树脂分别用5％浓度的碱、酸浸泡洗涤后在用无离子水淋洗阳树脂到PH＝4，阴树脂到PH＝9，阴、阳树脂柱串洗到出水电导率达50mn/cm2以下即电阻2万欧姆以上时备用；Step 7.3: Preparation of non-ionized water: using 5 times the volume of the tap water of the resin to flow through the anion and cation exchange columns from top to bottom, and measuring the conductivity of the water by using a conductivity meter for a plurality of times, and after being qualified, it is put into the ion-free water tank for use; When the conductivity of the effluent rises to 50 mn/cm 2 , that is, when the resistance is lower than 20,000 ohms, the production of ion-free water is stopped, and regeneration is awaited; the cation and the cation resin are respectively washed with 5% alkali and acid, and then used in the ion-free water. Leaching the cation resin to PH=4, anion resin to PH=9, the resin column of the yin and yang resin is washed until the conductivity of the effluent reaches 50mn/cm 2 or less, that is, when the resistance is more than 20,000 ohms;
步骤7.4、对糖化液进行离子交换处理：糖液经板式换热器降温到60℃以下后，在自上而下穿过流经阳、阴离交柱，顶出的水含糖在3％时，开始收集糖液到离交后贮罐；糖顶水后，控制离交的流速，检测出糖的电导率，当电阻值低于5万欧姆时停止离交；糖液离交结束后，用水顶糖到浓度3％时停止收集，用水洗到无糖后等待再生；用4倍于阳离子树脂体积的5％浓度盐酸浸泡8小时后，用无离子水洗到PH＝4；用4倍于阴离子树脂体积的5％浓度盐酸浸泡8小时后，用无离子水洗到PH＝9以下；阴、阳离子树脂柱串洗到电导率在50mn/cm2以下，待用。Step 7.4: Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the cation off the column from top to bottom, and the water is 3% in the water. At the beginning, the sugar liquid is collected to the storage tank after the separation; after the sugar top water, the flow rate of the separation is controlled, the conductivity of the sugar is detected, and the separation is stopped when the resistance value is less than 50,000 ohms; Stop collecting with water top sugar to a concentration of 3%, wash with water until sugar-free and wait for regeneration; soak for 8 hours with 5% concentration hydrochloric acid of 4 times the volume of cationic resin, wash with PH water to pH=4; use 4 times After soaking for 8 hours in a 5% strength hydrochloric acid volume of the anion resin, it was washed with ion-free water to a pH of 9 or less; the anion and cationic resin columns were washed in series to have a conductivity of 50 nm/cm 2 or less, and were used.
步骤8中对离子交换后的糖化液进行蒸发处理具体为：将蒸发器的供汽压力调整到5-6kg/cm2以上，开动打料泵，使糖化液依次进入一效、二效、三效蒸发室后，启动真空泵抽真空，在各效的真空度依次达到要求，慢慢开启蒸汽阀进行蒸发，经常用糖量计从取样口处取样检查浓度，在浓度达要求后向成品罐打料；蒸发结束时，用水顶糖到含糖<3％时，停止水顶糖，打开真空阀，破掉真空，关闭蒸汽，循环3分钟后，依次停机。In step 8, the ion-exchanged saccharification solution is subjected to evaporation treatment, specifically: adjusting the steam supply pressure of the evaporator to 5-6 kg/cm 2 or more, and starting the feed pump, so that the saccharification liquid enters the first effect, the second effect, and the third. After the evaporation chamber is started, the vacuum pump is started to vacuum, and the vacuum degree of each effect is sequentially reached. The steam valve is slowly opened for evaporation. The sugar meter is often used to sample the concentration from the sampling port, and the concentration is required to be sent to the finished product tank. At the end of evaporation, when the sugar is topped with sugar to <3%, stop the water top sugar, open the vacuum valve, break the vacuum, turn off the steam, and cycle for 3 minutes, then stop sequentially.
本发明的有益效果是：用玉米代替淀粉生产啤酒酿造专用糖浆可使吨糖 成本下降300-500元。用玉米代替淀粉生产啤酒酿造专用糖浆，可增加啤酒发酵用的氮源，起到改善啤酒风味，提高啤酒质量的作用。The beneficial effects of the invention are: using corn instead of starch to produce beer brewing special syrup can make tons of sugar The cost is reduced by 300-500 yuan. The use of corn instead of starch to produce beer-making syrup can increase the nitrogen source for beer fermentation, and improve the beer flavor and improve the quality of beer.
具体实施方式detailed description
下面结合具体实施方式对本发明进行详细说明。The present invention will be described in detail below in conjunction with specific embodiments.
本发明提供一种基于玉米直接法的啤酒酿造糖浆生产方法，包括以下步骤：The invention provides a beer brewing syrup production method based on the direct corn method, comprising the following steps:
步骤1、对玉米进行脱皮脱胚，制备成玉米粉；脱皮脱胚工艺为常规的技术，得到的玉米粉的指标为：细度40目全部通过，60目80％通过；胚芽、皮得率<18％；玉米粉收得率≥80％；土石、杂草≤1％；玉米粉脂肪含量<0.1％；玉米粉水分<15％。Step 1. The corn is peeled and de-emerged to prepare corn flour; the peeling and de-emergence process is a conventional technique, and the obtained corn flour has the following indexes: fineness of 40 meshes all passed, 60 meshes of 80% pass; germ and skin yield <18%; corn flour yield ≥ 80%; earth and stone, weeds ≤ 1%; corn flour fat content < 0.1%; corn flour moisture < 15%.
步骤2、对玉米粉进行调浆处理；在调浆罐内加入一定量的食品级用水；加蒸汽升温到56℃-60℃之间；按质量浓度为0.1％-0.5％加入CaCl2；开动调浆罐的搅拌后，由物料仓连续加玉米粉入池，玉米粉与食品级用水的质量百分比为38％-40％，调整PH在5.8-6.0之间后，加入ɑ-淀粉酶，其添加量为6υ/g，搅拌均匀。Step 2: mixing the corn flour; adding a certain amount of food grade water in the slurry tank; adding steam to the temperature between 56 ° C and 60 ° C; adding CaCl 2 according to the mass concentration of 0.1% - 0.5%; After the mixing tank is stirred, the corn powder is continuously added to the tank from the material silo, the mass percentage of corn flour and food grade water is 38%-40%, and after adjusting the pH between 5.8-6.0, the ɑ-amylase is added, The amount of addition was 6 υ / g, and the mixture was evenly stirred.
步骤3、对调浆后的玉米粉进行液化；调整液化器的供汽压力为6kg/cm2；检查管路阀门处于打料工作位置，开动调浆打料泵；开启喷射液化器蒸汽进口，调整蒸汽进量，同时开启进料阀门，控制进料流量；喷射温度始终保持在106℃-108℃；经喷射后的液化液按先后顺序一次进入多个液化液保温罐中，搅拌20分钟后取样测DE值，DE值在18％-20％之间，标志反应结束，得到液化液，液化液的浓度在20％-25％之间，液化液的pH值为5.2-5.3。Step 3. Liquefy the corn flour after slurry adjustment; adjust the steam supply pressure of the liquefier to 6kg/cm 2 ; check that the pipeline valve is in the working position of the material, start the slurry pump; turn on the steam inlet of the jet liquefier, adjust Steam intake, at the same time open the feed valve, control the feed flow; the injection temperature is always maintained at 106 ° C -108 ° C; the liquefied liquid after the injection into the liquefied liquid holding tank once in sequence, after stirring for 20 minutes, sampling The DE value is measured, and the DE value is between 18% and 20%. The reaction is completed, and the liquefied liquid is obtained. The concentration of the liquefied liquid is between 20% and 25%, and the pH of the liquefied liquid is 5.2 to 5.3.
步骤4、对液化液进行过滤除渣；启动油泵压紧，过滤,启动打料泵，进料压力3kg/cm2；过滤结束后，用热水冲洗过滤并到残糖的质量百分比<3％， 得到的滤液色泽浅黄色，透光度为≥90％，玉米蛋白渣含水≤60％。Step 4: Filter and remove the slag from the liquefied liquid; start the oil pump to compact, filter, start the pump, and feed the pressure 3kg/cm 2 ; after the filtration, rinse with hot water and the percentage of residual sugar is <3% The obtained filtrate has a light yellow color, a transmittance of ≥90%, and a zein residue of ≤60%.
步骤5、对过滤后的滤液进行糖化得到糖化液：将糖化缸洗净后蒸汽高压灭菌20分钟，其中，压力为4kg/cm2；液化液经板式换热器泵入糖化罐后，调整温度为52℃-55℃，调pH在5.2-5.3之间；依次加入真菌酶、普鲁兰酶和中性蛋白酶并搅拌，其中，真菌酶、普鲁兰酶和中性蛋白酶的添加量分别为2-4υ/g、0.5L/M3糖化液和0.1L/M3糖化液；间隔30分钟搅拌2分钟；每隔4个小时取样测DE值，DE≥50时终止糖化，得到的糖化液中麦芽糖≥50％、麦芽三糖≤23％以及葡萄糖≤10％。Step 5: saccharifying the filtered filtrate to obtain a saccharification solution: the saccharification tank is washed and steam autoclaved for 20 minutes, wherein the pressure is 4 kg/cm 2 ; the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger, and then adjusted. The temperature is 52 ° C -55 ° C, the pH is adjusted between 5.2-5.3; the fungal enzyme, pullulanase and neutral protease are added in sequence and stirred, wherein the addition amount of fungal enzyme, pullulanase and neutral protease respectively It is 2-4 υ / g, 0.5 L / M 3 saccharification solution and 0.1 L / M 3 saccharification solution; stirring for 2 minutes at intervals of 30 minutes; DE value is measured every 4 hours, saccharification is terminated when DE ≥ 50, and the obtained saccharification Maltose in the liquid ≥ 50%, maltotriose ≤ 23% and glucose ≤ 10%.
步骤6、对糖化液进行脱色过滤；往脱色罐内加入活性炭，其中，糖化液和活性炭的质量比为100:2，维持85℃30分钟后，打入过滤机内开始过滤，在透光率达到98％以上后开始向离交前贮罐进料，直至滤并残糖<3％时过滤结束，得到的滤液具体为色泽无色或微黄色，透光度>98％，色值<0.05，损失率<2％。Step 6. Decolorize and filter the saccharification solution; add activated carbon to the decolorization tank, wherein the mass ratio of the saccharification solution and the activated carbon is 100:2, and after maintaining the temperature at 85 ° C for 30 minutes, the filter is driven into the filter to start the filtration. After reaching 98% or more, the feed to the pre-discharge tank is started until the filtration and the residual sugar is <3%, the filtration is finished, and the obtained filtrate is colorless or yellowish, the transmittance is >98%, and the color value is <0.05. The loss rate is <2%.
步骤7、对脱色后的糖化液进行离子交换处理；Step 7. Perform ion exchange treatment on the decolorized saccharified solution;
步骤7.1、再生处理液的配制，制备质量浓度为10％的氯化钠溶液，制备质量浓度为5％的盐酸溶液和氢氧化钠溶液；Step 7.1, preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;
步骤7.2、新树脂处理，将阴、阳离子交换树脂分别从离交柱视镜孔中装入柱内；从立交柱上部进水，洗树脂到澄清透明；用10％的氯化钠溶液从柱上浸泡离子交换树脂到20小时后，用水洗到无色透明；阳、阴树脂分别用上述盐酸溶液和氢氧化钠溶液反复两次浸泡，每次8小时，在用水串洗达到要求后待用；Step 7.2, new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; the cation and anion resin are respectively soaked twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, and after being washed with water, the requirements are ready for use. ;
步骤7.3、无离子水的制备：用5倍于树脂体积的自来水自上而下流经阴、阳离子交换柱，多次用电导仪测水电导率，待合格(符合国家规定即可) 后，打入无离子水缸备用；当出水电导率上升到50mn/cm2时，即电阻低于2万欧姆时停止制作无离子水，等待再生；阴、阳树脂分别用5％浓度的碱、酸浸泡洗涤后在用无离子水淋洗阳树脂到PH＝4，阴树脂到PH＝9，阴、阳树脂柱串洗到出水电导率达50mn/cm2以下即电阻2万欧姆以上时备用；Step 7.3. Preparation of non-ionized water: use tap water of 5 times the volume of the resin to flow through the anion and cation exchange columns from top to bottom, and measure the conductivity of the water with a conductivity meter several times. After being qualified (in accordance with national regulations), Into the ion-free water tank for standby; when the conductivity of the effluent rises to 50mn/cm 2 , that is, when the resistance is lower than 20,000 ohms, the production of non-ionized water is stopped, waiting for regeneration; the yin and yang resins are soaked with 5% alkali and acid respectively. After washing, the cation resin is rinsed with ion-free water to PH=4, the anion resin to PH=9, and the resin column of the anode and the yang is washed until the conductivity of the effluent reaches 50 mn/cm 2 or less, that is, when the resistance is 20,000 ohm or more;
步骤7.4、对糖化液进行离子交换处理：糖液经板式换热器降温到60℃以下后，在自上而下穿过流经阳、阴离交柱，顶出的水含糖在3％时，开始收集糖液到离交后贮罐；糖顶水后，控制离交的流速，检测出糖的电导率，当电阻值低于5万欧姆时停止离交；糖液离交结束后，用水顶糖到浓度3％时停止收集，用水洗到无糖后等待再生；用4倍于阳离子树脂体积的5％浓度盐酸浸泡8小时后，用无离子水洗到PH＝4；用4倍于阴离子树脂体积的5％浓度盐酸浸泡8小时后，用无离子水洗到PH＝9以下；阴、阳离子树脂柱串洗到电导率在50mn/cm2以下，待用。Step 7.4: Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the cation off the column from top to bottom, and the water is 3% in the water. At the beginning, the sugar liquid is collected to the storage tank after the separation; after the sugar top water, the flow rate of the separation is controlled, the conductivity of the sugar is detected, and the separation is stopped when the resistance value is less than 50,000 ohms; Stop collecting with water top sugar to a concentration of 3%, wash with water until sugar-free and wait for regeneration; soak for 8 hours with 5% concentration hydrochloric acid of 4 times the volume of cationic resin, wash with PH water to pH=4; use 4 times After soaking for 8 hours in a 5% strength hydrochloric acid volume of the anion resin, it was washed with ion-free water to a pH of 9 or less; the anion and cationic resin columns were washed in series to have a conductivity of 50 nm/cm 2 or less, and were used.
步骤8、对离子交换后的糖化液进行蒸发处理：将蒸发器的供汽压力调整到5-6kg/cm2以上，开动打料泵，使糖化液依次进入一效、二效、三效蒸发室后，启动真空泵抽真空，在各效的真空度依次达到要求，慢慢开启蒸汽阀进行蒸发，用糖量计从取样口处取样检查浓度，在浓度达要求后向成品罐打料；蒸发结束时，用水顶糖到含糖<3％时，停止水顶糖，打开真空阀，破掉真空，关闭蒸汽，循环3分钟后，依次停机；Step 8. Evaporating the ion-exchanged saccharification solution: adjusting the steam supply pressure of the evaporator to 5-6 kg/cm 2 or more, and starting the feed pump, so that the saccharification liquid sequentially enters the first-, second-, and third-effect evaporation. After the chamber, the vacuum pump is started to evacuate, and the vacuum degree of each effect is sequentially reached. The steam valve is slowly opened for evaporation, and the concentration is sampled from the sampling port by the sugar meter, and the concentration is required to feed the finished product tank after evaporation; At the end, when the sugar is topped with sugar to <3%, stop the water top sugar, open the vacuum valve, break the vacuum, turn off the steam, and cycle for 3 minutes, then stop sequentially;
步骤9、成品包装，制备得到成品啤酒酿造用糖浆。Step 9. The finished product is packaged to prepare a finished syrup for beer brewing.
采用上述制备工艺制备得到的啤酒酿造糖浆的感官指标为：The sensory index of the brewed syrup prepared by the above preparation process is:
外观：呈粘稠状透明液体，无肉眼可见杂质；Appearance: It is a viscous transparent liquid with no visible impurities;
色泽：淡黄色或微棕黄色；Color: light yellow or slightly brownish yellow;
香气：具有啤酒酿造用糖的正常气味； Aroma: has the normal smell of sugar for beer brewing;
滋味：舒润纯正，无异味。Taste: Shurun is pure and has no odor.
本啤酒酿造糖浆的理化指标如表1所示：The physical and chemical indicators of this beer brewing syrup are shown in Table 1:
表1啤酒酿造糖浆的理化指标Table 1 Physical and chemical indicators of beer brewing syrup
指标等级Indicator level 优级Gifted class 一级First level 二级Secondary
固形物含量1％Solid content 1% ≥75≥75 ≥74≥74 ≥73≥73
PHPH 5.2-5.35.2-5.3 5.2-5.35.2-5.3 5.2-5.35.2-5.3
DE％DE% ≥50≥50 ≥52≥52 ≥55≥55
麦芽三糖含量1％Malt trisaccharide content 1% ≥23≥23 ≥21≥21 ≥20≥20
葡萄糖含量1％Glucose content 1% ≥10≥10 ≥12≥12 ≥14≥14
灰分1％Ash 1% ≤0.5≤0.5 ≤0.8≤0.8 ≤1.2≤1.2
本啤酒酿造糖浆的卫生指标如下所示：The hygienic indicators of this beer brewing syrup are as follows:
砷(mg/kg)≤0.5Arsenic (mg/kg) ≤ 0.5
铅(mg/kg)≤1.0Lead (mg/kg) ≤ 1.0
细菌总数(个/g)≤3000The total number of bacteria (pieces / g) ≤ 3000
大肠菌群(个/100g)≤30Coliform group (unit / 100g) ≤ 30
致病菌(指肠道致病菌及致病性球菌)：无Pathogenic bacteria (intestinal pathogens and pathogenic cocci): none
黄曲霉毒素：无。Aflatoxin: None.
该技术成果是在多年以淀粉为原料在生产淀粉糖浆、麦芽糖浆、果葡糖浆的基础上，经用玉米粉反复在生产中实践，成功地开发出用玉米直接法生产啤酒酿造专用糖浆的生产工艺技术，节省了玉米经制淀粉后制糖的环节，达到了降低制糖成本，提高经济效益的目的。同时，为啤酒工业提供了优质的酿造原料，为啤酒酿造节约了大量的粮食，使啤酒生产企业降低了生产成 本，还有，玉米直接法生产的啤酒酿造糖浆中含有丰富的氮源，这对提高啤酒质量，改善啤酒风味将起到重要的作用。这一点是用淀粉做原料生产啤酒酿造糖浆的产品是做不到的。原因是玉米制淀粉过程中需长时间浸泡和水洗，将析出的游离态蛋白全部都洗掉了，这样在糖化时即使加入蛋白酶，由于糖液中没有蛋白而不能转化为氮。而玉米直接法中玉米粉是用机器粉碎而得到的，玉米分在制糖液化时有游离态蛋白析出，与糖化加入蛋白酶作用后，产生了氮，有了氮源，就会更好的改善啤酒的风味，提高啤酒的质量，为啤酒生产企业带来更好的经济效益。The technical achievement is based on the production of starch syrup, maltose syrup and fructose syrup on the basis of starch for many years. Through the practice of repeatedly using corn flour in production, the production of special syrup for beer brewing by direct corn production has been successfully developed. The process technology saves the link of sugar production after corn starch production, and achieves the purpose of reducing the cost of sugar production and improving economic benefits. At the same time, it provides high-quality brewing raw materials for the beer industry, saving a lot of grain for beer brewing, and reducing the production of beer production enterprises. In addition, the beer brewing syrup produced by the direct method of corn is rich in nitrogen source, which plays an important role in improving beer quality and improving beer flavor. This is not possible with starch as a raw material for the production of beer-making syrup. The reason is that the corn starch process requires long-term soaking and water washing, and all the free proteins precipitated are washed away, so that even if the protease is added during the saccharification, the protein cannot be converted into nitrogen because there is no protein in the sugar liquid. In the direct corn method, the corn flour is obtained by machine pulverization. When the corn is liquefied, there is a free protein precipitated. When the saccharification is added to the protease, nitrogen is produced. With the nitrogen source, the beer is better. The flavor, improve the quality of beer, bring better economic benefits to beer manufacturers.
玉米直接法生产啤酒酿造糖浆，使粮食就地转化，就地升值称为了现实，既方便了农民卖粮，同时也为农产品增值开辟了新的途径。Direct corn production of beer brewing syrup, so that the local conversion of food, the local appreciation is called the reality, which not only facilitates farmers to sell grain, but also opens up new ways for the value of agricultural products.
本方法工艺中关键使用了五种酶制剂：淀粉酶、真菌酶、普鲁兰酶、蛋白酶、脂肪酶；PH值对酶的活性影响很大，生产中要严格控制PH值，每一种酶制剂都有自己最佳使用状态下的PH值，既不能过低，也不能过高，否则都将影响酶制剂的活性和酶反映效率。因此，在实际生产中要严格控料液的PH值，在必须进行调整PH值时,应先调PH值后加入酶。酶制剂的作用温度应选最佳作用温度，温度既不能过低，而使酶反映变慢，温度过高导致酶变性失去活性。Five enzyme preparations are used in the process: amylase, fungal enzyme, pullulanase, protease, lipase; PH value has a great influence on the activity of the enzyme, and the pH value should be strictly controlled in the production, each enzyme The preparation has its own pH value under the best use condition, neither too low nor too high, otherwise it will affect the activity of the enzyme preparation and the efficiency of enzyme reaction. Therefore, in actual production, the PH value of the feed liquid should be strictly controlled. When the pH value must be adjusted, the pH should be adjusted first and then the enzyme should be added. The temperature of the enzyme preparation should be selected at the optimum temperature. The temperature should not be too low, but the enzyme reaction should be slow. If the temperature is too high, the enzyme denaturation will lose activity.
实施例1Example 1
基于玉米直接法的啤酒酿造糖浆生产方法，包括以下步骤：A beer brewing syrup production method based on the direct corn method, comprising the following steps:
步骤1、对玉米进行脱皮脱胚，制备成玉米粉；脱皮脱胚工艺为常规的技术，得到的玉米粉的指标为：细度40目全部通过，60目80％通过；胚芽、皮得率<18％；玉米粉收得率≥80％；土石、杂草≤1％；玉米粉脂肪含量<0.1％；玉米粉水分<15％。 Step 1. The corn is peeled and de-emerged to prepare corn flour; the peeling and de-emergence process is a conventional technique, and the obtained corn flour has the following indexes: fineness of 40 meshes all passed, 60 meshes of 80% pass; germ and skin yield <18%; corn flour yield ≥ 80%; earth and stone, weeds ≤ 1%; corn flour fat content < 0.1%; corn flour moisture < 15%.
步骤2、对玉米粉进行调浆处理；在调浆罐内加入一定量的食品级用水；加蒸汽升温到58℃之间；按质量浓度为0.3％加入CaCl2；开动调浆罐的搅拌后，由物料仓连续加玉米粉入池，玉米粉与食品级用水的质量百分比为39％，调整PH在5.8-6.0之间后，加入ɑ-淀粉酶，其添加量为6υ/g，搅拌均匀。Step 2: Mixing the corn flour; adding a certain amount of food grade water in the pulping tank; adding steam to the temperature of 58 ° C; adding CaCl 2 according to the mass concentration of 0.3%; and stirring the mixing tank The corn powder is added to the tank continuously from the material warehouse. The mass percentage of corn flour and food grade water is 39%. After the pH is adjusted between 5.8 and 6.0, the ɑ-amylase is added, and the addition amount is 6υ/g, and the mixture is evenly stirred. .
步骤3、对调浆后的玉米粉进行液化；调整液化器的供汽压力为6kg/cm2；检查管路阀门处于打料工作位置，开动调浆打料泵；开启喷射液化器蒸汽进口，调整蒸汽进量，同时开启进料阀门，控制进料流量；喷射温度始终保持在107℃；经喷射后的液化液按先后顺序一次进入多个液化液保温罐中，搅拌20分钟后取样测DE值，DE值在18％-20％之间，标志反应结束，得到液化液，液化液的浓度为23％之间，液化液的pH值为5.2。Step 3. Liquefy the corn flour after slurry adjustment; adjust the steam supply pressure of the liquefier to 6kg/cm 2 ; check that the pipeline valve is in the working position of the material, start the slurry pump; turn on the steam inlet of the jet liquefier, adjust Steam intake, at the same time open the feed valve, control the feed flow; the injection temperature is always maintained at 107 ° C; the liquefied liquid after injection into the liquefied liquid insulation tank once in sequence, after stirring for 20 minutes, sample and measure the DE value The DE value is between 18% and 20%, indicating that the reaction is completed, and the liquefied liquid is obtained. The concentration of the liquefied liquid is between 23%, and the pH of the liquefied liquid is 5.2.
步骤4、对液化液进行过滤除渣；启动油泵压紧，过滤,启动打料泵，进料压力3kg/cm2；过滤结束后，用热水冲洗过滤并到残糖的质量百分比<3％，得到的滤液色泽浅黄色，透光度为≥90％，玉米蛋白渣含水≤60％。Step 4: Filter and remove the slag from the liquefied liquid; start the oil pump to compact, filter, start the pump, and feed the pressure 3kg/cm 2 ; after the filtration, rinse with hot water and the percentage of residual sugar is <3% The obtained filtrate has a light yellow color, a transmittance of ≥90%, and a zein residue of ≤60%.
步骤5、对过滤后的滤液进行糖化得到糖化液：将糖化缸洗净后蒸汽高压灭菌20分钟，其中，压力为4kg/cm2；液化液经板式换热器泵入糖化罐后，调整温度为54℃，调pH在5.2-5.3之间；依次加入真菌酶、普鲁兰酶和中性蛋白酶并搅拌，其中，真菌酶、普鲁兰酶和中性蛋白酶的添加量分别为3υ/g、0.5L/M3糖化液和0.1L/M3糖化液；间隔30分钟搅拌2分钟；每隔4个小时取样测DE值，DE≥50时终止糖化，得到的糖化液中麦芽糖≥50％、麦芽三糖≤23％以及葡萄糖≤10％。Step 5: saccharifying the filtered filtrate to obtain a saccharification solution: the saccharification tank is washed and steam autoclaved for 20 minutes, wherein the pressure is 4 kg/cm 2 ; the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger, and then adjusted. The temperature is 54 ° C, the pH is adjusted between 5.2-5.3; the fungal enzyme, pullulanase and neutral protease are added in sequence and stirred, wherein the addition amount of fungal enzyme, pullulanase and neutral protease is 3υ/ g, 0.5L/M 3 saccharification solution and 0.1L/M 3 saccharification solution; stir for 2 minutes at intervals of 30 minutes; sample DE value every 4 hours, terminate saccharification when DE ≥ 50, get maltose ≥ 50 in saccharification solution %, maltotriose ≤ 23% and glucose ≤ 10%.
步骤6、对糖化液进行脱色过滤；往脱色罐内加入活性炭，其中，糖化液和活性炭的质量比为100:2，维持85℃30分钟后，打入过滤机内开始过滤，在透光率达到98％以上后开始向离交前贮罐进料，直至滤并残糖<3％时过滤 结束，得到的滤液具体为色泽无色或微黄色，透光度>98％，色值<0.05，损失率<2％。Step 6. Decolorize and filter the saccharification solution; add activated carbon to the decolorization tank, wherein the mass ratio of the saccharification solution and the activated carbon is 100:2, and after maintaining the temperature at 85 ° C for 30 minutes, the filter is driven into the filter to start the filtration. After reaching 98% or more, the feed to the pre-discharge tank is started until the filtration and residual sugar are <3%. At the end, the obtained filtrate was specifically colorless or yellowish in color, transmittance was >98%, color value was <0.05, and loss rate was <2%.
步骤7、对脱色后的糖化液进行离子交换处理；Step 7. Perform ion exchange treatment on the decolorized saccharified solution;
步骤7.1、再生处理液的配制，制备质量浓度为10％的氯化钠溶液，制备质量浓度为5％的盐酸溶液和氢氧化钠溶液；Step 7.1, preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;
步骤7.2、新树脂处理，将阴、阳离子交换树脂分别从离交柱视镜孔中装入柱内；从立交柱上部进水，洗树脂到澄清透明；用10％的氯化钠溶液从柱上浸泡离子交换树脂到20小时后，用水洗到无色透明；阳、阴树脂分别用上述盐酸溶液和氢氧化钠溶液反复两次浸泡，每次8小时，在用水串洗达到要求后待用；Step 7.2, new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; the cation and anion resin are respectively soaked twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, and after being washed with water, the requirements are ready for use. ;
步骤7.3、无离子水的制备：用5倍于树脂体积的自来水自上而下流经阴、阳离子交换柱，多次用电导仪测水电导率，待合格(符合国家规定即可)后，打入无离子水缸备用；当出水电导率上升到50mn/cm2时，即电阻低于2万欧姆时停止制作无离子水，等待再生；阴、阳树脂分别用5％浓度的碱、酸浸泡洗涤后在用无离子水淋洗阳树脂到PH＝4，阴树脂到PH＝9，阴、阳树脂柱串洗到出水电导率达50mn/cm2以下即电阻2万欧姆以上时备用；Step 7.3. Preparation of non-ionized water: use 5 times of the volume of the tap water to flow through the anion and cation exchange columns from top to bottom, and measure the conductivity of the water with a conductivity meter several times. After being qualified (in accordance with national regulations), Into the ion-free water tank for standby; when the conductivity of the effluent rises to 50mn/cm 2 , that is, when the resistance is lower than 20,000 ohms, the production of non-ionized water is stopped, waiting for regeneration; the yin and yang resins are soaked with 5% alkali and acid respectively. After washing, the cation resin is rinsed with ion-free water to PH=4, the anion resin to PH=9, and the resin column of the anode and the yang is washed until the conductivity of the effluent reaches 50 mn/cm 2 or less, that is, when the resistance is 20,000 ohm or more;
步骤7.4、对糖化液进行离子交换处理：糖液经板式换热器降温到60℃以下后，在自上而下穿过流经阳、阴离交柱，顶出的水含糖在3％时，开始收集糖液到离交后贮罐；糖顶水后，控制离交的流速，检测出糖的电导率，当电阻值低于5万欧姆时停止离交；糖液离交结束后，用水顶糖到浓度3％时停止收集，用水洗到无糖后等待再生；用4倍于阳离子树脂体积的5％浓度盐酸浸泡8小时后，用无离子水洗到PH＝4；用4倍于阴离子树脂体积的5％浓度盐酸浸泡8小时后，用无离子水洗到PH＝9以下；阴、阳离子树脂柱 串洗到电导率在50mn/cm2以下，待用。Step 7.4: Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the cation off the column from top to bottom, and the water is 3% in the water. At the beginning, the sugar liquid is collected to the storage tank after the separation; after the sugar top water, the flow rate of the separation is controlled, the conductivity of the sugar is detected, and the separation is stopped when the resistance value is less than 50,000 ohms; Stop collecting with water top sugar to a concentration of 3%, wash with water until sugar-free and wait for regeneration; soak for 8 hours with 5% concentration hydrochloric acid of 4 times the volume of cationic resin, wash with PH water to pH=4; use 4 times After soaking for 8 hours in a 5% strength hydrochloric acid volume of the anion resin, it was washed with ion-free water to a pH of 9 or less; the anion and cationic resin columns were washed in series to have a conductivity of 50 nm/cm 2 or less, and were used.
步骤8、对离子交换后的糖化液进行蒸发处理：将蒸发器的供汽压力调整到5-6kg/cm2以上，开动打料泵，使糖化液依次进入一效、二效、三效蒸发室后，启动真空泵抽真空，在各效的真空度依次达到要求，慢慢开启蒸汽阀进行蒸发，用糖量计从取样口处取样检查浓度，在浓度达要求后向成品罐打料；蒸发结束时，用水顶糖到含糖<3％时，停止水顶糖，打开真空阀，破掉真空，关闭蒸汽，循环3分钟后，依次停机；Step 8. Evaporating the ion-exchanged saccharification solution: adjusting the steam supply pressure of the evaporator to 5-6 kg/cm 2 or more, and starting the feed pump, so that the saccharification liquid sequentially enters the first-, second-, and third-effect evaporation. After the chamber, the vacuum pump is started to evacuate, and the vacuum degree of each effect is sequentially reached. The steam valve is slowly opened for evaporation, and the concentration is sampled from the sampling port by the sugar meter, and the concentration is required to feed the finished product tank after evaporation; At the end, when the sugar is topped with sugar to <3%, stop the water top sugar, open the vacuum valve, break the vacuum, turn off the steam, and cycle for 3 minutes, then stop sequentially;
步骤9、成品包装，制备得到成品啤酒酿造用糖浆。Step 9. The finished product is packaged to prepare a finished syrup for beer brewing.
实施例2Example 2
基于玉米直接法的啤酒酿造糖浆生产方法，包括以下步骤：A beer brewing syrup production method based on the direct corn method, comprising the following steps:
步骤1、对玉米进行脱皮脱胚，制备成玉米粉；脱皮脱胚工艺为常规的技术，得到的玉米粉的指标为：细度40目全部通过，60目80％通过；胚芽、皮得率<18％；玉米粉收得率≥80％；土石、杂草≤1％；玉米粉脂肪含量<0.1％；玉米粉水分<15％。Step 1. The corn is peeled and de-emerged to prepare corn flour; the peeling and de-emergence process is a conventional technique, and the obtained corn flour has the following indexes: fineness of 40 meshes all passed, 60 meshes of 80% pass; germ and skin yield <18%; corn flour yield ≥ 80%; earth and stone, weeds ≤ 1%; corn flour fat content < 0.1%; corn flour moisture < 15%.
步骤2、对玉米粉进行调浆处理；在调浆罐内加入一定量的食品级用水；加蒸汽升温到56℃；按质量浓度为0.1％加入CaCl2；开动调浆罐的搅拌后，由物料仓连续加玉米粉入池，玉米粉与食品级用水的质量百分比为38％，按产量要求的加粉量加好后，调整PH在5.8-6.0之间后，加入ɑ-淀粉酶，其添加量为6υ/g，搅拌均匀。Step 2: mixing the corn flour; adding a certain amount of food grade water in the mixing tank; adding steam to the temperature of 56 ° C; adding CaCl 2 according to the mass concentration of 0.1%; after stirring the mixing tank, The material warehouse is continuously added with corn flour into the pool. The mass percentage of corn flour and food grade water is 38%. After the powder is added according to the output requirement, after adjusting the pH between 5.8 and 6.0, the ɑ-amylase is added. The amount of addition was 6 υ / g, and the mixture was evenly stirred.
步骤3、对调浆后的玉米粉进行液化；调整液化器的供汽压力为6kg/cm2；检查管路阀门处于打料工作位置，开动调浆打料泵；开启喷射液化器蒸汽进口，调整蒸汽进量，同时开启进料阀门，控制进料流量；喷射温度始终保持在106℃；经喷射后的液化液按先后顺序一次进入多个液化液保温罐中，搅 拌20分钟后取样测DE值，DE值在18％-20％之间，标志反应结束，得到液化液，液化液的浓度在20％-25％之间，液化液的pH值为5.2-5.3。Step 3. Liquefy the corn flour after slurry adjustment; adjust the steam supply pressure of the liquefier to 6kg/cm 2 ; check that the pipeline valve is in the working position of the material, start the slurry pump; turn on the steam inlet of the jet liquefier, adjust Steam intake, at the same time open the feed valve, control the feed flow; the injection temperature is always maintained at 106 ° C; the liquefied liquid after injection into the liquefied liquid insulation tank once in sequence, after stirring for 20 minutes, sample and measure the DE value The DE value is between 18% and 20%, indicating that the reaction is completed, and the liquefied liquid is obtained. The concentration of the liquefied liquid is between 20% and 25%, and the pH of the liquefied liquid is between 5.2 and 5.3.
步骤4、对液化液进行过滤除渣；启动油泵压紧，过滤,启动打料泵，进料压力3kg/cm2；过滤结束后，用热水冲洗过滤并到残糖的质量百分比<3％，得到的滤液色泽浅黄色，透光度为≥90％，玉米蛋白渣含水≤60％。Step 4: Filter and remove the slag from the liquefied liquid; start the oil pump to compact, filter, start the pump, and feed the pressure 3kg/cm 2 ; after the filtration, rinse with hot water and the percentage of residual sugar is <3% The obtained filtrate has a light yellow color, a transmittance of ≥90%, and a zein residue of ≤60%.
步骤5、对过滤后的滤液进行糖化得到糖化液：将糖化缸洗净后蒸汽高压灭菌20分钟，其中，压力为4kg/cm2；液化液经板式换热器泵入糖化罐后，调整温度为52℃，调pH在5.2-5.3之间；依次加入真菌酶、普鲁兰酶和中性蛋白酶并搅拌，其中，真菌酶、普鲁兰酶和中性蛋白酶的添加量分别为2υ/g、0.5L/M3糖化液和0.1L/M3糖化液；间隔30分钟搅拌2分钟；每隔4个小时取样测DE值，DE≥50时终止糖化，得到的糖化液中麦芽糖≥50％、麦芽三糖≤23％以及葡萄糖≤10％。Step 5: saccharifying the filtered filtrate to obtain a saccharification solution: the saccharification tank is washed and steam autoclaved for 20 minutes, wherein the pressure is 4 kg/cm 2 ; the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger, and then adjusted. The temperature is 52 ° C, the pH is adjusted between 5.2-5.3; the fungal enzyme, pullulanase and neutral protease are added in sequence and stirred, wherein the addition amount of fungal enzyme, pullulanase and neutral protease is 2υ/ g, 0.5L/M 3 saccharification solution and 0.1L/M 3 saccharification solution; stir for 2 minutes at intervals of 30 minutes; sample DE value every 4 hours, terminate saccharification when DE ≥ 50, get maltose ≥ 50 in saccharification solution %, maltotriose ≤ 23% and glucose ≤ 10%.
步骤6、对糖化液进行脱色过滤；往脱色罐内加入活性炭，其中，糖化液和活性炭的质量比为100:2，维持85℃30分钟后，打入过滤机内开始过滤，在透光率达到98％以上后开始向离交前贮罐进料，直至滤并残糖<3％时过滤结束，得到的滤液具体为色泽无色或微黄色，透光度>98％，色值<0.05，损失率<2％。Step 6. Decolorize and filter the saccharification solution; add activated carbon to the decolorization tank, wherein the mass ratio of the saccharification solution and the activated carbon is 100:2, and after maintaining the temperature at 85 ° C for 30 minutes, the filter is driven into the filter to start the filtration. After reaching 98% or more, the feed to the pre-discharge tank is started until the filtration and the residual sugar is <3%, the filtration is finished, and the obtained filtrate is colorless or yellowish, the transmittance is >98%, and the color value is <0.05. The loss rate is <2%.
步骤7、对脱色后的糖化液进行离子交换处理；Step 7. Perform ion exchange treatment on the decolorized saccharified solution;
步骤7.1、再生处理液的配制，制备质量浓度为10％的氯化钠溶液，制备质量浓度为5％的盐酸溶液和氢氧化钠溶液；Step 7.1, preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;
步骤7.2、新树脂处理，将阴、阳离子交换树脂分别从离交柱视镜孔中装入柱内；从立交柱上部进水，洗树脂到澄清透明；用10％的氯化钠溶液从柱上浸泡离子交换树脂到20小时后，用水洗到无色透明；阳、阴树脂分别 用上述盐酸溶液和氢氧化钠溶液反复两次浸泡，每次8小时，在用水串洗达到要求后待用；Step 7.2, new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; Repeatedly soaking twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, after being washed with water to meet the requirements;
步骤7.3、无离子水的制备：用5倍于树脂体积的自来水自上而下流经阴、阳离子交换柱，多次用电导仪测水电导率，待合格(符合国家规定即可)后，打入无离子水缸备用；当出水电导率上升到50mn/cm2时，即电阻低于2万欧姆时停止制作无离子水，等待再生；阴、阳树脂分别用5％浓度的碱、酸浸泡洗涤后在用无离子水淋洗阳树脂到PH＝4，阴树脂到PH＝9，阴、阳树脂柱串洗到出水电导率达50mn/cm2以下即电阻2万欧姆以上时备用；Step 7.3. Preparation of non-ionized water: use 5 times of the volume of the tap water to flow through the anion and cation exchange columns from top to bottom, and measure the conductivity of the water with a conductivity meter several times. After being qualified (in accordance with national regulations), Into the ion-free water tank for standby; when the conductivity of the effluent rises to 50mn/cm 2 , that is, when the resistance is lower than 20,000 ohms, the production of non-ionized water is stopped, waiting for regeneration; the yin and yang resins are soaked with 5% alkali and acid respectively. After washing, the cation resin is rinsed with ion-free water to PH=4, the anion resin to PH=9, and the resin column of the anode and the yang is washed until the conductivity of the effluent reaches 50 mn/cm 2 or less, that is, when the resistance is 20,000 ohm or more;
步骤7.4、对糖化液进行离子交换处理：糖液经板式换热器降温到60℃以下后，在自上而下穿过流经阳、阴离交柱，顶出的水含糖在3％时，开始收集糖液到离交后贮罐；糖顶水后，控制离交的流速，检测出糖的电导率，当电阻值低于5万欧姆时停止离交；糖液离交结束后，用水顶糖到浓度3％时停止收集，用水洗到无糖后等待再生；用4倍于阳离子树脂体积的5％浓度盐酸浸泡8小时后，用无离子水洗到PH＝4；用4倍于阴离子树脂体积的5％浓度盐酸浸泡8小时后，用无离子水洗到PH＝9以下；阴、阳离子树脂柱串洗到电导率在50mn/cm2以下，待用。Step 7.4: Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the cation off the column from top to bottom, and the water is 3% in the water. At the beginning, the sugar liquid is collected to the storage tank after the separation; after the sugar top water, the flow rate of the separation is controlled, the conductivity of the sugar is detected, and the separation is stopped when the resistance value is less than 50,000 ohms; Stop collecting with water top sugar to a concentration of 3%, wash with water until sugar-free and wait for regeneration; soak for 8 hours with 5% concentration hydrochloric acid of 4 times the volume of cationic resin, wash with PH water to pH=4; use 4 times After soaking for 8 hours in a 5% strength hydrochloric acid volume of the anion resin, it was washed with ion-free water to a pH of 9 or less; the anion and cationic resin columns were washed in series to have a conductivity of 50 nm/cm 2 or less, and were used.
步骤8、对离子交换后的糖化液进行蒸发处理：将蒸发器的供汽压力调整到5-6kg/cm2以上，开动打料泵，使糖化液依次进入一效、二效、三效蒸发室后，启动真空泵抽真空，在各效的真空度依次达到要求，慢慢开启蒸汽阀进行蒸发，用糖量计从取样口处取样检查浓度，在浓度达要求后向成品罐打料；蒸发结束时，用水顶糖到含糖<3％时，停止水顶糖，打开真空阀，破掉真空，关闭蒸汽，循环3分钟后，依次停机；Step 8. Evaporating the ion-exchanged saccharification solution: adjusting the steam supply pressure of the evaporator to 5-6 kg/cm 2 or more, and starting the feed pump, so that the saccharification liquid sequentially enters the first-, second-, and third-effect evaporation. After the chamber, the vacuum pump is started to evacuate, and the vacuum degree of each effect is sequentially reached. The steam valve is slowly opened for evaporation, and the concentration is sampled from the sampling port by the sugar meter, and the concentration is required to feed the finished product tank after evaporation; At the end, when the sugar is topped with sugar to <3%, stop the water top sugar, open the vacuum valve, break the vacuum, turn off the steam, and cycle for 3 minutes, then stop sequentially;
步骤9、成品包装，制备得到成品啤酒酿造用糖浆。 Step 9. The finished product is packaged to prepare a finished syrup for beer brewing.
实施例3Example 3
基于玉米直接法的啤酒酿造糖浆生产方法，包括以下步骤：A beer brewing syrup production method based on the direct corn method, comprising the following steps:
步骤1、对玉米进行脱皮脱胚，制备成玉米粉；脱皮脱胚工艺为常规的技术，得到的玉米粉的指标为：细度40目全部通过，60目80％通过；胚芽、皮得率<18％；玉米粉收得率≥80％；土石、杂草≤1％；玉米粉脂肪含量<0.1％；玉米粉水分<15％。Step 1. The corn is peeled and de-emerged to prepare corn flour; the peeling and de-emergence process is a conventional technique, and the obtained corn flour has the following indexes: fineness of 40 meshes all passed, 60 meshes of 80% pass; germ and skin yield <18%; corn flour yield ≥ 80%; earth and stone, weeds ≤ 1%; corn flour fat content < 0.1%; corn flour moisture < 15%.
步骤2、对玉米粉进行调浆处理；在调浆罐内加入一定量的食品级用水；加蒸汽升温到60℃之间；按质量浓度为0.5％加入CaCl2；开动调浆罐的搅拌后，由物料仓连续加玉米粉入池，玉米粉与食品级用水的质量百分比为40％，调整PH在5.8-6.0之间后，加入ɑ-淀粉酶，其添加量为6υ/g，搅拌均匀。Step 2: Mixing the corn flour; adding a certain amount of food grade water in the pulping tank; adding steam to the temperature of 60 ° C; adding CaCl 2 according to the mass concentration of 0.5%; and stirring the mixing tank The corn powder is added to the tank continuously from the material warehouse. The mass percentage of corn flour and food grade water is 40%. After adjusting the pH between 5.8 and 6.0, the ɑ-amylase is added, and the addition amount is 6υ/g, and the mixture is evenly stirred. .
步骤3、对调浆后的玉米粉进行液化；调整液化器的供汽压力为6kg/cm2；检查管路阀门处于打料工作位置，开动调浆打料泵；开启喷射液化器蒸汽进口，调整蒸汽进量，同时开启进料阀门，控制进料流量；喷射温度始终保持在108℃；经喷射后的液化液按先后顺序一次进入多个液化液保温罐中，搅拌20分钟后取样测DE值，DE值在18％-20％之间，标志反应结束，得到液化液，液化液的浓度在20％-25％之间，液化液的pH值为5.2-5.3。Step 3. Liquefy the corn flour after slurry adjustment; adjust the steam supply pressure of the liquefier to 6kg/cm 2 ; check that the pipeline valve is in the working position of the material, start the slurry pump; turn on the steam inlet of the jet liquefier, adjust Steam intake, at the same time open the feed valve, control the feed flow; the injection temperature is always maintained at 108 ° C; the liquefied liquid after injection into the liquefied liquid insulation tank once in sequence, after stirring for 20 minutes, sample and measure the DE value The DE value is between 18% and 20%, indicating that the reaction is completed, and the liquefied liquid is obtained. The concentration of the liquefied liquid is between 20% and 25%, and the pH of the liquefied liquid is between 5.2 and 5.3.
步骤4、对液化液进行过滤除渣；启动油泵压紧，过滤,启动打料泵，进料压力3kg/cm2；过滤结束后，用热水冲洗过滤并到残糖的质量百分比<3％，得到的滤液色泽浅黄色，透光度为≥90％，玉米蛋白渣含水≤60％。Step 4: Filter and remove the slag from the liquefied liquid; start the oil pump to compact, filter, start the pump, and feed the pressure 3kg/cm 2 ; after the filtration, rinse with hot water and the percentage of residual sugar is <3% The obtained filtrate has a light yellow color, a transmittance of ≥90%, and a zein residue of ≤60%.
步骤5、对过滤后的滤液进行糖化得到糖化液：将糖化缸洗净后蒸汽高压灭菌20分钟，其中，压力为4kg/cm2；液化液经板式换热器泵入糖化罐后，调整温度为54℃，调pH在5.2-5.3之间；依次加入真菌酶、普鲁兰酶和中性蛋白酶并搅拌，其中，真菌酶、普鲁兰酶和中性蛋白酶的添加量分别为 4υ/g、0.5L/M3糖化液和0.1L/M3糖化液；间隔30分钟搅拌2分钟；每隔4个小时取样测DE值，DE≥50时终止糖化，得到的糖化液中麦芽糖≥50％、麦芽三糖≤23％以及葡萄糖≤10％。Step 5: saccharifying the filtered filtrate to obtain a saccharification solution: the saccharification tank is washed and steam autoclaved for 20 minutes, wherein the pressure is 4 kg/cm 2 ; the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger, and then adjusted. The temperature is 54 ° C, the pH is adjusted between 5.2-5.3; the fungal enzyme, pullulanase and neutral protease are added in sequence and stirred, wherein the addition amount of fungal enzyme, pullulanase and neutral protease is 4υ/ g, 0.5L/M 3 saccharification solution and 0.1L/M 3 saccharification solution; stir for 2 minutes at intervals of 30 minutes; sample DE value every 4 hours, terminate saccharification when DE ≥ 50, get maltose ≥ 50 in saccharification solution %, maltotriose ≤ 23% and glucose ≤ 10%.
步骤6、对糖化液进行脱色过滤；往脱色罐内加入活性炭，其中，糖化液和活性炭的质量比为100:2，维持85℃30分钟后，打入过滤机内开始过滤，在透光率达到98％以上后开始向离交前贮罐进料，直至滤并残糖<3％时过滤结束，得到的滤液具体为色泽无色或微黄色，透光度>98％，色值<0.05，损失率<2％。Step 6. Decolorize and filter the saccharification solution; add activated carbon to the decolorization tank, wherein the mass ratio of the saccharification solution and the activated carbon is 100:2, and after maintaining the temperature at 85 ° C for 30 minutes, the filter is driven into the filter to start the filtration. After reaching 98% or more, the feed to the pre-discharge tank is started until the filtration and the residual sugar is <3%, the filtration is finished, and the obtained filtrate is colorless or yellowish, the transmittance is >98%, and the color value is <0.05. The loss rate is <2%.
步骤7、对脱色后的糖化液进行离子交换处理；Step 7. Perform ion exchange treatment on the decolorized saccharified solution;
步骤7.1、再生处理液的配制，制备质量浓度为10％的氯化钠溶液，制备质量浓度为5％的盐酸溶液和氢氧化钠溶液；Step 7.1, preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;
步骤7.2、新树脂处理，将阴、阳离子交换树脂分别从离交柱视镜孔中装入柱内；从立交柱上部进水，洗树脂到澄清透明；用10％的氯化钠溶液从柱上浸泡离子交换树脂到20小时后，用水洗到无色透明；阳、阴树脂分别用上述盐酸溶液和氢氧化钠溶液反复两次浸泡，每次8小时，在用水串洗达到要求后待用；Step 7.2, new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; the cation and anion resin are respectively soaked twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, and after being washed with water, the requirements are ready for use. ;
步骤7.3、无离子水的制备：用5倍于树脂体积的自来水自上而下流经阴、阳离子交换柱，多次用电导仪测水电导率，待合格(符合国家规定即可)后，打入无离子水缸备用；当出水电导率上升到50mn/cm2时，即电阻低于2万欧姆时停止制作无离子水，等待再生；阴、阳树脂分别用5％浓度的碱、酸浸泡洗涤后在用无离子水淋洗阳树脂到PH＝4，阴树脂到PH＝9，阴、阳树脂柱串洗到出水电导率达50mn/cm2以下即电阻2万欧姆以上时备用；Step 7.3. Preparation of non-ionized water: use 5 times of the volume of the tap water to flow through the anion and cation exchange columns from top to bottom, and measure the conductivity of the water with a conductivity meter several times. After being qualified (in accordance with national regulations), Into the ion-free water tank for standby; when the conductivity of the effluent rises to 50mn/cm 2 , that is, when the resistance is lower than 20,000 ohms, the production of non-ionized water is stopped, waiting for regeneration; the yin and yang resins are soaked with 5% alkali and acid respectively. After washing, the cation resin is rinsed with ion-free water to PH=4, the anion resin to PH=9, and the resin column of the anode and the yang is washed until the conductivity of the effluent reaches 50 mn/cm 2 or less, that is, when the resistance is 20,000 ohm or more;
步骤7.4、对糖化液进行离子交换处理：糖液经板式换热器降温到60℃ 以下后，在自上而下穿过流经阳、阴离交柱，顶出的水含糖在3％时，开始收集糖液到离交后贮罐；糖顶水后，控制离交的流速，检测出糖的电导率，当电阻值低于5万欧姆时停止离交；糖液离交结束后，用水顶糖到浓度3％时停止收集，用水洗到无糖后等待再生；用4倍于阳离子树脂体积的5％浓度盐酸浸泡8小时后，用无离子水洗到PH＝4；用4倍于阴离子树脂体积的5％浓度盐酸浸泡8小时后，用无离子水洗到PH＝9以下；阴、阳离子树脂柱串洗到电导率在50mn/cm2以下，待用。Step 7.4: Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the yin from the top to the bottom, and the water is 3% in the water. At the beginning, the sugar liquid is collected to the storage tank after the separation; after the sugar top water, the flow rate of the separation is controlled, the conductivity of the sugar is detected, and the separation is stopped when the resistance value is less than 50,000 ohms; Stop collecting with water top sugar to a concentration of 3%, wash with water until sugar-free and wait for regeneration; soak for 8 hours with 5% concentration hydrochloric acid of 4 times the volume of cationic resin, wash with PH water to pH=4; use 4 times After soaking for 8 hours in a 5% strength hydrochloric acid volume of the anion resin, it was washed with ion-free water to a pH of 9 or less; the anion and cationic resin columns were washed in series to have a conductivity of 50 nm/cm 2 or less, and were used.
步骤8、对离子交换后的糖化液进行蒸发处理：将蒸发器的供汽压力调整到5-6kg/cm2以上，开动打料泵，使糖化液依次进入一效、二效、三效蒸发室后，启动真空泵抽真空，在各效的真空度依次达到要求，慢慢开启蒸汽阀进行蒸发，用糖量计从取样口处取样检查浓度，在浓度达要求后向成品罐打料；蒸发结束时，用水顶糖到含糖<3％时，停止水顶糖，打开真空阀，破掉真空，关闭蒸汽，循环3分钟后，依次停机；Step 8. Evaporating the ion-exchanged saccharification solution: adjusting the steam supply pressure of the evaporator to 5-6 kg/cm 2 or more, and starting the feed pump, so that the saccharification liquid sequentially enters the first-, second-, and third-effect evaporation. After the chamber, the vacuum pump is started to evacuate, and the vacuum degree of each effect is sequentially reached. The steam valve is slowly opened for evaporation, and the concentration is sampled from the sampling port by the sugar meter, and the concentration is required to feed the finished product tank after evaporation; At the end, when the sugar is topped with sugar to <3%, stop the water top sugar, open the vacuum valve, break the vacuum, turn off the steam, and cycle for 3 minutes, then stop sequentially;
步骤9、成品包装，制备得到成品啤酒酿造用糖浆。 Step 9. The finished product is packaged to prepare a finished syrup for beer brewing.

Claims (9)

  1. 一种基于玉米直接法的啤酒酿造糖浆生产方法，其特征在于，具体按照以下步骤实施：A beer brewing syrup production method based on the direct corn method, which is characterized in that the following steps are specifically carried out:
    步骤1、对玉米进行脱皮脱胚，制备成玉米粉；Step 1. peeling and degerming the corn to prepare corn flour;
    步骤2、对玉米粉进行调浆处理；Step 2. Performing a slurry treatment on the corn flour;
    步骤3、对调浆后的玉米粉进行液化；Step 3: liquefying the corn flour after the pulping;
    步骤4、对液化液进行过滤除渣；Step 4: filtering and slaging the liquefied liquid;
    步骤5、对过滤后的滤液进行糖化，得到糖化液；Step 5, saccharifying the filtered filtrate to obtain a saccharification solution;
    步骤6、对糖化液进行脱色过滤；Step 6. Decolorize and filter the saccharification solution;
    步骤7、对脱色后的糖化液进行离子交换处理；Step 7. Perform ion exchange treatment on the decolorized saccharified solution;
    步骤8、对离子交换后的糖化液进行蒸发处理；Step 8. Perform evaporation treatment on the ion exchanged saccharification solution;
    步骤9、成品包装，制备得到成品啤酒酿造用糖浆。Step 9. The finished product is packaged to prepare a finished syrup for beer brewing.
  2. 根据权利要求1所述的基于玉米直接法的啤酒酿造糖浆生产方法，其特征在于，所述步骤1中制备得到的玉米粉具体为：细度40目全部通过，60目80％通过；胚芽、皮得率<18％；玉米粉收得率≥80％；土石、杂草≤1％；玉米粉脂肪含量<0.1％；玉米粉水分<15％。The method for producing a brewing syrup based on the corn direct method according to claim 1, wherein the corn flour prepared in the step 1 is specifically: fineness of 40 meshes all passed, 60 meshes of 80% passed; germ, The skin yield is <18%; the corn flour yield is ≥80%; the earth and stone, weeds ≤1%; the corn flour fat content is <0.1%; the corn flour moisture is <15%.
  3. 根据权利要求1所述的基于玉米直接法的啤酒酿造糖浆生产方法，其特征在于，所述步骤2中对玉米粉进行调浆处理具体为：在调浆罐内加入一定量的食品级用水；加蒸汽升温到56℃-60℃之间；按质量浓度为0.1％-0.5％加入CaCl2，开动调浆罐的搅拌后，由物料仓连续加玉米粉入池，玉米粉与食品级用水的质量百分比为38％-40％，调整玉米粉浆的PH在5.8-6.0之间后，加入ɑ-淀粉酶，其中，ɑ-淀粉酶的添加量为6υ/g，搅拌均匀。The method for producing a beer brewing syrup based on the corn direct method according to claim 1, wherein the step of adjusting the corn flour in the step 2 is specifically: adding a certain amount of food grade water into the pulping tank; Add steam to the temperature between 56 °C and 60 °C; add CaCl 2 according to the mass concentration of 0.1%-0.5%, start the mixing of the mixing tank, and then add corn flour into the pool continuously from the material silo, corn flour and food grade water. The mass percentage is 38%-40%. After adjusting the pH of the corn flour to 5.8-6.0, the ɑ-amylase is added, wherein the ɑ-amylase is added in an amount of 6 υ/g, and the mixture is evenly stirred.
  4. 根据权利要求1所述的基于玉米直接法的啤酒酿造糖浆生产方法，其 特征在于，所述步骤3中对调浆后的玉米粉进行液化具体为：调整液化器的供汽压力为6kg/cm2，检查管路阀门处于打料工作位置，开动调浆打料泵；开启喷射液化器蒸汽进口，调整蒸汽进量，同时开启进料阀门，控制进料流量；喷射温度始终保持在106℃-108℃；经喷射后的液化液按先后顺序一次进入多个液化液保温罐中，搅拌20分钟后取样测DE值，DE值在18％-20％之间，标志反应结束，得到液化液，液化液的浓度在20％-25％之间，液化液的pH值为5.2-5.3。The method for producing a brewing syrup based on the corn direct method according to claim 1, wherein the step of liquefying the pulverized corn flour is specifically: adjusting the liquefaction pressure of the liquefier to 6 kg/cm 2 Check the pipeline valve in the working position, start the slurry pump; turn on the steam inlet of the jet liquefier, adjust the steam intake, and open the feed valve to control the feed flow; the injection temperature is always maintained at 106 °C-108 °C; After the injection, the liquefied liquid enters a plurality of liquefied liquid holding tanks in sequence, and after stirring for 20 minutes, the DE value is sampled and measured, and the DE value is between 18% and 20%, indicating that the reaction is finished, and the liquefied liquid is obtained, and liquefaction is obtained. The concentration of the liquid is between 20% and 25%, and the pH of the liquefied liquid is between 5.2 and 5.3.
  5. 根据权利要求1所述的基于玉米直接法的啤酒酿造糖浆生产方法，其特征在于，所述步骤4中对液化液进行过滤除渣具体为：启动油泵压紧，过滤,启动打料泵，进料压力3kg/cm2；过滤结束后，用热水冲洗过滤并到残糖的质量百分比<3％，得到的滤液色泽浅黄色，透光度为≥90％，玉米蛋白渣含水≤60％。The method for producing a brewing syrup based on the corn direct method according to claim 1, wherein the step of filtering and slaging the liquefied liquid in the step 4 is: starting the oil pump to compact, filtering, starting the pump, and entering The pressure of the material is 3kg/cm 2 ; after the filtration is completed, the filtration is carried out with hot water and the mass percentage of the residual sugar is <3%, and the obtained filtrate has a light yellow color, a transmittance of ≥90%, and a zein residue of ≤60%.
  6. 根据权利要求1所述的基于玉米直接法的啤酒酿造糖浆生产方法，其特征在于，所述步骤5中对过滤后的滤液进行糖化得到糖化液具体为：将糖化缸洗净后蒸汽高压灭菌20分钟，其中，压力为4kg/cm2；液化液经板式换热器泵入糖化罐后，调整温度为52℃-55℃，调pH在5.2-5.3之间；依次加入真菌酶、普鲁兰酶和中性蛋白酶并搅拌，其中，真菌酶、普鲁兰酶和中性蛋白酶的添加量分别为2-4υ/g、0.5L/M3糖化液和0.1L/M3糖化液；间隔30分钟搅拌2分钟；每隔4个小时取样测DE值，DE≥50时终止糖化，得到的糖化液中麦芽糖≥50％、麦芽三糖≤23％以及葡萄糖≤10％。The method for producing a brewing syrup based on the corn direct method according to claim 1, wherein in the step 5, the filtered filtrate is saccharified to obtain a saccharifying liquid, which is specifically: steam autoclaving after washing the saccharification tank 20 minutes, wherein the pressure is 4kg/cm 2 ; after the liquefied liquid is pumped into the saccharification tank through the plate heat exchanger, the temperature is adjusted to 52 ° C -55 ° C, and the pH is adjusted between 5.2 and 5.3; the fungal enzyme is added in turn. Lanase and neutral protease are stirred, wherein the addition amount of fungal enzyme, pullulanase and neutral protease is 2-4υ/g, 0.5L/M 3 saccharification solution and 0.1L/M 3 saccharification solution; Stir for 2 minutes in 30 minutes; DE value is measured every 4 hours, and saccharification is terminated when DE ≥ 50, and the obtained saccharification solution has maltose ≥ 50%, maltotriose ≤ 23%, and glucose ≤ 10%.
  7. 根据权利要求1所述的基于玉米直接法的啤酒酿造糖浆生产方法，其特征在于，所述步骤6中对糖化液进行脱色过滤具体为：往脱色罐内加入活性炭，糖化液和活性炭的质量比为100:2，维持85℃30分钟后，打入过滤机 内开始过滤，在透光率达到98％以上后开始向离交前贮罐进料，直至滤并残糖<3％时过滤结束，得到的滤液具体为色泽无色或微黄色，透光度>98％，色值<0.05，损失率<2％。The method for producing a brewing syrup based on the corn direct method according to claim 1, wherein the decolorizing and filtering of the saccharifying solution in the step 6 is specifically: adding a mass ratio of the activated carbon, the saccharifying solution and the activated carbon into the decolorizing tank. 100:2, after maintaining at 85 ° C for 30 minutes, drive into the filter Filtration starts within the filter, and after the light transmittance reaches 98% or more, the feed to the pre-discharge tank is started until the filtration and residual sugar <3%, the filtration is finished, and the obtained filtrate is colorless or yellowish, and the transmittance is transparent. >98%, color value <0.05, loss rate <2%.
  8. 根据权利要求1所述的基于玉米直接法的啤酒酿造糖浆生产方法，其特征在于，所述步骤7中对脱色后的糖化液进行离子交换处理具体为：The method for producing a brewing syrup based on the corn direct method according to claim 1, wherein the ion exchange treatment of the decolorized saccharified solution in the step 7 is as follows:
    步骤7.1、再生处理液的配制，制备质量浓度为10％的氯化钠溶液，制备质量浓度为5％的盐酸溶液和氢氧化钠溶液；Step 7.1, preparation of the regeneration treatment liquid, preparing a sodium chloride solution having a mass concentration of 10%, preparing a hydrochloric acid solution and a sodium hydroxide solution having a mass concentration of 5%;
    步骤7.2、新树脂处理，将阴、阳离子交换树脂分别从离交柱视镜孔中装入柱内；从立交柱上部进水，洗树脂到澄清透明；用10％的氯化钠溶液从柱上浸泡离子交换树脂到20小时后，用水洗到无色透明；阳、阴树脂分别用上述盐酸溶液和氢氧化钠溶液反复两次浸泡，每次8小时，在用水串洗达到要求后待用；Step 7.2, new resin treatment, the anion and cation exchange resin are respectively loaded into the column from the aperture of the mirror; the water is poured from the upper part of the column to wash the resin to clear and transparent; the column is removed from the column with 10% sodium chloride solution. After soaking the ion exchange resin for 20 hours, wash it with water until it is colorless and transparent; the cation and anion resin are respectively soaked twice with the above hydrochloric acid solution and sodium hydroxide solution for 8 hours each time, and after being washed with water, the requirements are ready for use. ;
    步骤7.3、无离子水的制备：用5倍于树脂体积的自来水自上而下流经阴、阳离子交换柱，多次用电导仪测水电导率，待合格后，打入无离子水缸备用；当出水电导率上升到50mn/cm2时，即电阻低于2万欧姆时停止制作无离子水，等待再生；阴、阳树脂分别用5％浓度的碱、酸浸泡洗涤后在用无离子水淋洗阳树脂到PH＝4，阴树脂到PH＝9，阴、阳树脂柱串洗到出水电导率达50mn/cm2以下即电阻2万欧姆以上时备用；Step 7.3: Preparation of non-ionized water: using 5 times the volume of the tap water of the resin to flow through the anion and cation exchange columns from top to bottom, and measuring the conductivity of the water by using a conductivity meter for a plurality of times, and after being qualified, it is put into the ion-free water tank for use; When the conductivity of the effluent rises to 50 mn/cm 2 , that is, when the resistance is lower than 20,000 ohms, the production of ion-free water is stopped, and regeneration is awaited; the cation and the cation resin are respectively washed with 5% alkali and acid, and then used in the ion-free water. Leaching the cation resin to PH=4, anion resin to PH=9, the resin column of the yin and yang resin is washed until the conductivity of the effluent reaches 50mn/cm 2 or less, that is, when the resistance is more than 20,000 ohms;
    步骤7.4、对糖化液进行离子交换处理：糖液经板式换热器降温到60℃以下后，在自上而下穿过流经阳、阴离交柱，顶出的水含糖在3％时，开始收集糖液到离交后贮罐；糖顶水后，控制离交的流速，检测出糖的电导率，当电阻值低于5万欧姆时停止离交；糖液离交结束后，用水顶糖到浓度3％时停止收集，用水洗到无糖后等待再生；用4倍于阳离子树脂体积的5％浓 度盐酸浸泡8小时后，用无离子水洗到PH＝4；用4倍于阴离子树脂体积的5％浓度盐酸浸泡8小时后，用无离子水洗到PH＝9以下；阴、阳离子树脂柱串洗到电导率在50mn/cm2以下，待用。Step 7.4: Ion exchange treatment of the saccharification solution: after the sugar liquid is cooled to below 60 ° C by the plate heat exchanger, the water flows through the cation and the cation off the column from top to bottom, and the water is 3% in the water. At the beginning, the sugar liquid is collected to the storage tank after the separation; after the sugar top water, the flow rate of the separation is controlled, the conductivity of the sugar is detected, and the separation is stopped when the resistance value is less than 50,000 ohms; Stop collecting with water top sugar to a concentration of 3%, wash with water until sugar-free and wait for regeneration; soak for 8 hours with 5% concentration hydrochloric acid of 4 times the volume of cationic resin, wash with PH water to pH=4; use 4 times After soaking for 8 hours in a 5% strength hydrochloric acid volume of the anion resin, it was washed with ion-free water to a pH of 9 or less; the anion and cationic resin columns were washed in series to have a conductivity of 50 nm/cm 2 or less, and were used.
  9. 根据权利要求1所述的基于玉米直接法的啤酒酿造糖浆生产方法，其特征在于，所述步骤8中对离子交换后的糖化液进行蒸发处理具体为：将蒸发器的供汽压力调整到5-6kg/cm2以上，开动打料泵，使糖化液依次进入一效、二效、三效蒸发室后，启动真空泵抽真空，在各效的真空度依次达到要求，慢慢开启蒸汽阀进行蒸发，用糖量计从取样口处取样检查浓度，在浓度达要求后向成品罐打料；蒸发结束时，用水顶糖到含糖<3％时，停止水顶糖，打开真空阀，破掉真空，关闭蒸汽，循环3分钟后，依次停机。 The method for producing a brewing syrup based on the corn direct method according to claim 1, wherein in the step 8, the saccharification liquid after the ion exchange is subjected to evaporation treatment, specifically: adjusting the steam supply pressure of the evaporator to 5 -6kg/cm 2 or more, start the pump, so that the saccharification liquid enters the one-effect, two-effect, three-effect evaporation chamber in turn, start the vacuum pump to vacuum, and the vacuum degree of each effect is sequentially achieved, and the steam valve is slowly opened. Evaporate, check the concentration from the sampling port with a sugar meter, and feed the finished product tank after the concentration reaches the requirement; at the end of the evaporation, when the sugar is topped with sugar to <3%, stop the water top sugar, open the vacuum valve, break The vacuum is turned off, the steam is turned off, and after 3 minutes of circulation, the machine is stopped in sequence.
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CN115466760A (en) * 2022-08-16 2022-12-13 广州双桥（重庆）有限公司 Preparation method of starch syrup
CN116874144A (en) * 2023-09-07 2023-10-13 北京博泰至淳生物科技有限公司 Method for recycling brewing yellow water in high value
CN116874144B (en) * 2023-09-07 2023-12-01 北京博泰至淳生物科技有限公司 Method for recycling brewing yellow water in high value
CN117587084A (en) * 2023-11-29 2024-02-23 山东省鲁洲食品集团有限公司 Method capable of reducing pH drop in saccharification process of maltose syrup production

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