WO2010014797A3 - Integrated system for productioin of biofuel feedstock - Google Patents

Integrated system for productioin of biofuel feedstock Download PDF

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Publication number
WO2010014797A3
WO2010014797A3 PCT/US2009/052234 US2009052234W WO2010014797A3 WO 2010014797 A3 WO2010014797 A3 WO 2010014797A3 US 2009052234 W US2009052234 W US 2009052234W WO 2010014797 A3 WO2010014797 A3 WO 2010014797A3
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WO
WIPO (PCT)
Prior art keywords
heterotrophic
production
phototrophic
processes
integrating
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PCT/US2009/052234
Other languages
French (fr)
Other versions
WO2010014797A2 (en
Inventor
Zhanyou Chi
Yubin Zheng
Ben Lucker
Shulin Chen
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Washington State University Research Foundation
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Publication date
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Priority to CN2009801281461A priority Critical patent/CN102089434A/en
Publication of WO2010014797A2 publication Critical patent/WO2010014797A2/en
Publication of WO2010014797A3 publication Critical patent/WO2010014797A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/18—Open ponds; Greenhouse type or underground installations
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/30—Wastewater or sewage treatment systems using renewable energies
    • Y02W10/37—Wastewater or sewage treatment systems using renewable energies using solar energy

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Disclosed is a culture system for the production of algae biomass to obtain lipid, protein and carbohydrate. By integrating heterotrophic processes with a phototrophic process in parallel, this system provides year around production in colder climates. By integrating heterotrophic processes with a phototrophic process in series, this system creates a two-stage, separated mixed- trophic algal process that uses organic carbon and nutrients for the production of seed in the heterotrophic process, followed by release of cultured seed in large-scale phototrophic culture for cell biomass accumulation. Organic carbon source including waste materials can be used to feed the heterotrophic process. The production capacity ratio between the heterotrophic and the phototrophic processes can be adjusted according to season and according to the availability of related resources. The systems are used for producing and harvesting an algal biofuel feedstock as well as other potential high-value products. The sequence and approach enhances utilization of carbon and nutrient waste-streams, provides an effective method for controlling contamination, adds flexibility in regard to production and type of available products, and supplies greater economic viability due to maximized use of available growth surface areas.
PCT/US2009/052234 2008-07-30 2009-07-30 Integrated system for productioin of biofuel feedstock WO2010014797A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2009801281461A CN102089434A (en) 2008-07-30 2009-07-30 Integrated system for productioin of biofuel feedstock

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8470808P 2008-07-30 2008-07-30
US61/084,708 2008-07-30

Publications (2)

Publication Number Publication Date
WO2010014797A2 WO2010014797A2 (en) 2010-02-04
WO2010014797A3 true WO2010014797A3 (en) 2010-04-29

Family

ID=41610953

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/052234 WO2010014797A2 (en) 2008-07-30 2009-07-30 Integrated system for productioin of biofuel feedstock

Country Status (2)

Country Link
CN (1) CN102089434A (en)
WO (1) WO2010014797A2 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1393061B1 (en) * 2008-10-23 2012-04-11 Eni Spa INTEGRATED PROCEDURE FOR THE PRODUCTION OF BIO-OIL FROM MICRO-ORGANISMS
IT1396940B1 (en) * 2009-12-09 2012-12-20 Eni Spa PROCEDURE FOR THE PRODUCTION OF BIO-OIL FROM PHOTOTROPHE AND ETEROTROFE ALGAE
WO2012065545A1 (en) * 2010-11-16 2012-05-24 华东理工大学 Microalgae culturing method for oil and lutein rapid accumulation
CN102154110B (en) * 2011-01-27 2016-03-02 华东理工大学 A kind of microalgae culture method of high yield
EP2500324A1 (en) * 2011-03-18 2012-09-19 Koenraad Vanhoutte Apparatus for the microbiological treatment of water
CN102994363A (en) * 2011-09-17 2013-03-27 中国科学院兰州化学物理研究所 Device for aerated culture of heterotrophic-photoautotrophic microbes via series connection
CN103103128A (en) * 2011-11-10 2013-05-15 中国石油化工股份有限公司 Method for high efficiency enrichment culture of microalgae
US9090862B2 (en) 2012-12-21 2015-07-28 Ductor Oy System and method for processing biological material
US20190241847A1 (en) * 2014-10-22 2019-08-08 Gsr Solutions Llc Symbiotic algae system
WO2016064803A1 (en) * 2014-10-22 2016-04-28 Gsr Solutions Llc Symbiotic algae system with looped reactor
US10900013B2 (en) 2014-10-22 2021-01-26 Gsr Solutions, Llc Systems and methods of producing compositions from the nutrients recovered from waste streams
CN105671095A (en) * 2016-02-27 2016-06-15 昆明理工大学 Cultivation method for promoting rapid accumulation of oil producing microalgae cells and grease
DE102017218001B4 (en) * 2017-10-10 2022-06-02 GICON GROßMANN INGENIEUR CONSULT GMBH Method and system for the heterotrophic and mixotrophic cultivation of microalgae
CN108641914B (en) * 2018-03-27 2021-09-21 贵州民族大学 Circulating chlorella breeds separating centrifuge
CN109052832A (en) * 2018-08-10 2018-12-21 上海大学 The processing method of the waste water generated during Preparation of biodiesel from waste oils
FR3085960B1 (en) * 2018-09-14 2020-11-27 Kyanos Biotechnologies PROCESS FOR CULTURE OF A MICROORGANISM OF INTEREST AND ASSOCIATED INSTALLATION
CN110468025A (en) * 2019-09-06 2019-11-19 王习羽 It is a kind of suitable for heterotrophism and the micro algae culturing device and cultural method of mixotrophic cultivation
CN113136345B (en) * 2020-01-19 2023-05-05 中国石油化工股份有限公司 Method for heterotrophic-autotrophic continuous cultivation of photosynthetic microorganisms, cultivation system and application thereof
CN113136321A (en) * 2020-01-19 2021-07-20 中国石油化工股份有限公司 Method and system for heterotrophic-autotrophic co-culture of photosynthetic microorganisms and method for production of biomass and bioenergy
CN113136339B (en) * 2020-01-19 2023-07-14 中国石油化工股份有限公司 Method for continuously culturing photosynthetic microorganisms by mixotrophic-autotrophic culture, culture system and application thereof
CN115029248A (en) * 2022-06-21 2022-09-09 昆明理工大学 Method for improving microalgae lipid yield by utilizing recycled wastewater

Citations (5)

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Publication number Priority date Publication date Assignee Title
US20040121447A1 (en) * 2002-11-07 2004-06-24 Real Fournier Method and apparatus for concentrating an aqueous suspension of microalgae
US20080086939A1 (en) * 2006-10-13 2008-04-17 Dunlop Eric H High photoefficiency microalgae bioreactors
US20080086938A1 (en) * 2006-10-13 2008-04-17 Hazlebeck David A Photosynthetic carbon dioxide sequestration and pollution abatement
US20080124446A1 (en) * 2006-06-28 2008-05-29 Michael Markels Method of production of biofuel from the surface of the open ocean
US20080160593A1 (en) * 2006-12-29 2008-07-03 Oyler James R Two-stage process for producing oil from microalgae

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040121447A1 (en) * 2002-11-07 2004-06-24 Real Fournier Method and apparatus for concentrating an aqueous suspension of microalgae
US20080124446A1 (en) * 2006-06-28 2008-05-29 Michael Markels Method of production of biofuel from the surface of the open ocean
US20080086939A1 (en) * 2006-10-13 2008-04-17 Dunlop Eric H High photoefficiency microalgae bioreactors
US20080086938A1 (en) * 2006-10-13 2008-04-17 Hazlebeck David A Photosynthetic carbon dioxide sequestration and pollution abatement
US20080160593A1 (en) * 2006-12-29 2008-07-03 Oyler James R Two-stage process for producing oil from microalgae

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WO2010014797A2 (en) 2010-02-04
CN102089434A (en) 2011-06-08

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