WO2010004338A3 - Proteins comprising affinity tag for two distinct targets - Google Patents

Proteins comprising affinity tag for two distinct targets Download PDF

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Publication number
WO2010004338A3
WO2010004338A3 PCT/GB2009/050817 GB2009050817W WO2010004338A3 WO 2010004338 A3 WO2010004338 A3 WO 2010004338A3 GB 2009050817 W GB2009050817 W GB 2009050817W WO 2010004338 A3 WO2010004338 A3 WO 2010004338A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
affinity tag
distinct targets
tag
targets
Prior art date
Application number
PCT/GB2009/050817
Other languages
French (fr)
Other versions
WO2010004338A2 (en
Inventor
David Peter Joseph Hornby
Original Assignee
The University Of Sheffield
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The University Of Sheffield filed Critical The University Of Sheffield
Publication of WO2010004338A2 publication Critical patent/WO2010004338A2/en
Publication of WO2010004338A3 publication Critical patent/WO2010004338A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

The invention relates to an affinity tag comprising a mosaic of a first polypeptide tag and a second polypeptide tag.
PCT/GB2009/050817 2008-07-10 2009-07-09 Affinity tags WO2010004338A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0812651A GB2461728A (en) 2008-07-10 2008-07-10 Affinity tags comprised of copolymers of two monomeric units with affinity for distinct capture reagents
GB0812651.8 2008-07-10

Publications (2)

Publication Number Publication Date
WO2010004338A2 WO2010004338A2 (en) 2010-01-14
WO2010004338A3 true WO2010004338A3 (en) 2010-04-01

Family

ID=39722076

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2009/050817 WO2010004338A2 (en) 2008-07-10 2009-07-09 Affinity tags

Country Status (2)

Country Link
GB (1) GB2461728A (en)
WO (1) WO2010004338A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3066685B8 (en) 2013-11-05 2020-08-05 ABB Power Grids Switzerland AG Ground system for high voltage semiconductor valve
WO2016145416A2 (en) * 2015-03-11 2016-09-15 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050032173A1 (en) * 2003-08-05 2005-02-10 Mauricio Rojas Fusion proteins with a membrane translocating sequence and methods of using same to inhibit an immune response
WO2005089933A1 (en) * 2004-03-17 2005-09-29 Dynal Biotech Asa Improvements in polymer particles
WO2009093962A1 (en) * 2008-01-24 2009-07-30 Ge Healthcare Bio-Sciences Ab Multifunctional tags

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2832422B1 (en) * 2001-11-21 2004-09-03 Bio Merieux NUCLEOTIDE SEQUENCE ENCODING A MODIFIED PROTEIN OF INTEREST, EXPRESSION VECTOR AND PROCESS FOR OBTAINING SAME
US7238478B2 (en) * 2003-11-13 2007-07-03 Stratagene California Compositions and methods for protein isolation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050032173A1 (en) * 2003-08-05 2005-02-10 Mauricio Rojas Fusion proteins with a membrane translocating sequence and methods of using same to inhibit an immune response
WO2005089933A1 (en) * 2004-03-17 2005-09-29 Dynal Biotech Asa Improvements in polymer particles
WO2009093962A1 (en) * 2008-01-24 2009-07-30 Ge Healthcare Bio-Sciences Ab Multifunctional tags

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BERNAUDAT FLORENT ET AL: "Combined hydrophobic-metal binding fusion tags for applications in aqueous two-phase partitioning.", PROTEIN EXPRESSION AND PURIFICATION APR 2006, vol. 46, no. 2, April 2006 (2006-04-01), pages 438 - 445, XP024908696, ISSN: 1046-5928 *
K BECKER ET AL: "Multipurpose peptide tags for protein isolation", JOURNAL OF CHROMATOGRAPHY A, vol. 1202, 5 July 2008 (2008-07-05), pages 40 - 46, XP023172436, Retrieved from the Internet <URL:http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TG8-4SX9G03-1-K&_cdi=5248&_user=987766&_pii=S0021967308010923&_orig=browse&_coverDate=08%2F15%2F2008&_sk=987979998&view=c&wchp=dGLzVzz-zSkzV&md5=d8bafd61eb88201424d8111308168716&ie=/sdarticle.pdf> [retrieved on 20100201], DOI: 10.1016/j.chroma.2008.06.045 *

Also Published As

Publication number Publication date
GB0812651D0 (en) 2008-08-20
GB2461728A (en) 2010-01-13
WO2010004338A2 (en) 2010-01-14

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