WO2009152520A3 - Nanovolume microcapillary crystallization system - Google Patents

Nanovolume microcapillary crystallization system Download PDF

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Publication number
WO2009152520A3
WO2009152520A3 PCT/US2009/047414 US2009047414W WO2009152520A3 WO 2009152520 A3 WO2009152520 A3 WO 2009152520A3 US 2009047414 W US2009047414 W US 2009047414W WO 2009152520 A3 WO2009152520 A3 WO 2009152520A3
Authority
WO
WIPO (PCT)
Prior art keywords
crystallization
nanovolume
microcapillary
screening
allows
Prior art date
Application number
PCT/US2009/047414
Other languages
French (fr)
Other versions
WO2009152520A2 (en
Inventor
Cory Gerdts
Mark Elliott
Peter Nollert-Von-Specht
Lansing J. Stewart
Original Assignee
Decode Biostructures, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Decode Biostructures, Inc. filed Critical Decode Biostructures, Inc.
Priority to EP09763807A priority Critical patent/EP2352591A4/en
Priority to JP2011513753A priority patent/JP2011525166A/en
Publication of WO2009152520A2 publication Critical patent/WO2009152520A2/en
Publication of WO2009152520A3 publication Critical patent/WO2009152520A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D9/00—Crystallisation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/20—Jet mixers, i.e. mixers using high-speed fluid streams
    • B01F25/23—Mixing by intersecting jets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/06—Crystallising dishes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B29/00—Single crystals or homogeneous polycrystalline material with defined structure characterised by the material or by their shape
    • C30B29/54—Organic compounds
    • C30B29/58—Macromolecular compounds
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B7/00—Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Materials Engineering (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Metallurgy (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Peptides Or Proteins (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

A nanovolume microcapillary crystallization system allows nanoliter-volume screening of crystallization conditions in a crystal card that allows crystals to either be removed for traditional cryoprotection or in situ X-ray diffraction studies on protein crystals that grow within. The system integrates formulation of crystallization cocktails with preparation of the crystallization experiments. The system allows the researcher to select either gradient screening in crystallization experiments for efficient exploration of crystallization phase space or a combination of sparse matrix with gradient screening to execute one comprehensive hybrid crystallization trial.
PCT/US2009/047414 2008-06-13 2009-06-15 Nanovolume microcapillary crystallization system WO2009152520A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP09763807A EP2352591A4 (en) 2008-06-13 2009-06-15 Nanovolume microcapillary crystallization system
JP2011513753A JP2011525166A (en) 2008-06-13 2009-06-15 Nanovolume microcapillary crystallization system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6153608P 2008-06-13 2008-06-13
US61/061,536 2008-06-13

Publications (2)

Publication Number Publication Date
WO2009152520A2 WO2009152520A2 (en) 2009-12-17
WO2009152520A3 true WO2009152520A3 (en) 2010-02-18

Family

ID=41417428

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/047414 WO2009152520A2 (en) 2008-06-13 2009-06-15 Nanovolume microcapillary crystallization system

Country Status (3)

Country Link
EP (1) EP2352591A4 (en)
JP (2) JP2011525166A (en)
WO (1) WO2009152520A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
WO2011120024A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet generation for droplet-based assays
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
GB0907504D0 (en) * 2009-04-30 2009-06-10 Imp Innovations Ltd Method of producing crystalline protein
CN113856235A (en) * 2021-09-29 2021-12-31 浙江大华技术股份有限公司 Cooling crystallization control method, device, electronic equipment and system

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5598933A (en) * 1994-04-29 1997-02-04 Phoenix International Life Sciences Inc. Method for extraction, extraction cartridge and automated extraction processing system
US20040115731A1 (en) * 2001-04-06 2004-06-17 California Institute Of Technology Microfluidic protein crystallography
US20050205005A1 (en) * 2001-04-06 2005-09-22 California Institute Of Technology Microfluidic protein crystallography
US20060003439A1 (en) * 2004-07-02 2006-01-05 Ismagilov Rustem F Microfluidic system
US20060027780A1 (en) * 2004-07-12 2006-02-09 Decode Biostructures, Inc. Crystallization solutions
US20060094119A1 (en) * 2004-10-29 2006-05-04 Ismagilov Rustem F Microfluidic system

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2309130B1 (en) * 1999-06-28 2016-08-10 California Institute of Technology Microfabricated elastomeric valve and pump systems
JP4855680B2 (en) * 2002-05-09 2012-01-18 ザ・ユニバーシティ・オブ・シカゴ Apparatus and method for transport and reaction by pressure driven plug
WO2004035751A2 (en) * 2002-10-18 2004-04-29 The Regents Of The University Of California Methods for crystallographic structure determination employing hydrogen exchange analysis
CN101914803B (en) * 2004-01-25 2012-07-04 弗卢丁公司 Crystal forming devices and systems and methods for making and using the same
JP2008538077A (en) * 2005-03-16 2008-10-09 ユニバーシティ オブ シカゴ Micro fluidic system
US8986449B2 (en) * 2006-06-28 2015-03-24 Microlytic North America Inc. Device and a method for promoting crystallisation

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5598933A (en) * 1994-04-29 1997-02-04 Phoenix International Life Sciences Inc. Method for extraction, extraction cartridge and automated extraction processing system
US20040115731A1 (en) * 2001-04-06 2004-06-17 California Institute Of Technology Microfluidic protein crystallography
US20050205005A1 (en) * 2001-04-06 2005-09-22 California Institute Of Technology Microfluidic protein crystallography
US20060003439A1 (en) * 2004-07-02 2006-01-05 Ismagilov Rustem F Microfluidic system
US20060027780A1 (en) * 2004-07-12 2006-02-09 Decode Biostructures, Inc. Crystallization solutions
US20060094119A1 (en) * 2004-10-29 2006-05-04 Ismagilov Rustem F Microfluidic system

Also Published As

Publication number Publication date
EP2352591A2 (en) 2011-08-10
EP2352591A4 (en) 2013-01-23
JP2014240343A (en) 2014-12-25
JP2011525166A (en) 2011-09-15
WO2009152520A2 (en) 2009-12-17

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