WO2009152520A3 - Nanovolume microcapillary crystallization system - Google Patents
Nanovolume microcapillary crystallization system Download PDFInfo
- Publication number
- WO2009152520A3 WO2009152520A3 PCT/US2009/047414 US2009047414W WO2009152520A3 WO 2009152520 A3 WO2009152520 A3 WO 2009152520A3 US 2009047414 W US2009047414 W US 2009047414W WO 2009152520 A3 WO2009152520 A3 WO 2009152520A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- crystallization
- nanovolume
- microcapillary
- screening
- allows
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D9/00—Crystallisation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
- B01F25/20—Jet mixers, i.e. mixers using high-speed fluid streams
- B01F25/23—Mixing by intersecting jets
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/06—Crystallising dishes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
- C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
-
- C—CHEMISTRY; METALLURGY
- C30—CRYSTAL GROWTH
- C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
- C30B29/00—Single crystals or homogeneous polycrystalline material with defined structure characterised by the material or by their shape
- C30B29/54—Organic compounds
- C30B29/58—Macromolecular compounds
-
- C—CHEMISTRY; METALLURGY
- C30—CRYSTAL GROWTH
- C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
- C30B7/00—Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0877—Flow chambers
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Metallurgy (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
- Peptides Or Proteins (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
A nanovolume microcapillary crystallization system allows nanoliter-volume screening of crystallization conditions in a crystal card that allows crystals to either be removed for traditional cryoprotection or in situ X-ray diffraction studies on protein crystals that grow within. The system integrates formulation of crystallization cocktails with preparation of the crystallization experiments. The system allows the researcher to select either gradient screening in crystallization experiments for efficient exploration of crystallization phase space or a combination of sparse matrix with gradient screening to execute one comprehensive hybrid crystallization trial.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP09763807A EP2352591A4 (en) | 2008-06-13 | 2009-06-15 | Nanovolume microcapillary crystallization system |
JP2011513753A JP2011525166A (en) | 2008-06-13 | 2009-06-15 | Nanovolume microcapillary crystallization system |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US6153608P | 2008-06-13 | 2008-06-13 | |
US61/061,536 | 2008-06-13 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009152520A2 WO2009152520A2 (en) | 2009-12-17 |
WO2009152520A3 true WO2009152520A3 (en) | 2010-02-18 |
Family
ID=41417428
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2009/047414 WO2009152520A2 (en) | 2008-06-13 | 2009-06-15 | Nanovolume microcapillary crystallization system |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP2352591A4 (en) |
JP (2) | JP2011525166A (en) |
WO (1) | WO2009152520A2 (en) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
US10512910B2 (en) | 2008-09-23 | 2019-12-24 | Bio-Rad Laboratories, Inc. | Droplet-based analysis method |
WO2011120024A1 (en) | 2010-03-25 | 2011-09-29 | Quantalife, Inc. | Droplet generation for droplet-based assays |
US11130128B2 (en) | 2008-09-23 | 2021-09-28 | Bio-Rad Laboratories, Inc. | Detection method for a target nucleic acid |
GB0907504D0 (en) * | 2009-04-30 | 2009-06-10 | Imp Innovations Ltd | Method of producing crystalline protein |
CN113856235A (en) * | 2021-09-29 | 2021-12-31 | 浙江大华技术股份有限公司 | Cooling crystallization control method, device, electronic equipment and system |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5598933A (en) * | 1994-04-29 | 1997-02-04 | Phoenix International Life Sciences Inc. | Method for extraction, extraction cartridge and automated extraction processing system |
US20040115731A1 (en) * | 2001-04-06 | 2004-06-17 | California Institute Of Technology | Microfluidic protein crystallography |
US20050205005A1 (en) * | 2001-04-06 | 2005-09-22 | California Institute Of Technology | Microfluidic protein crystallography |
US20060003439A1 (en) * | 2004-07-02 | 2006-01-05 | Ismagilov Rustem F | Microfluidic system |
US20060027780A1 (en) * | 2004-07-12 | 2006-02-09 | Decode Biostructures, Inc. | Crystallization solutions |
US20060094119A1 (en) * | 2004-10-29 | 2006-05-04 | Ismagilov Rustem F | Microfluidic system |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2309130B1 (en) * | 1999-06-28 | 2016-08-10 | California Institute of Technology | Microfabricated elastomeric valve and pump systems |
JP4855680B2 (en) * | 2002-05-09 | 2012-01-18 | ザ・ユニバーシティ・オブ・シカゴ | Apparatus and method for transport and reaction by pressure driven plug |
WO2004035751A2 (en) * | 2002-10-18 | 2004-04-29 | The Regents Of The University Of California | Methods for crystallographic structure determination employing hydrogen exchange analysis |
CN101914803B (en) * | 2004-01-25 | 2012-07-04 | 弗卢丁公司 | Crystal forming devices and systems and methods for making and using the same |
JP2008538077A (en) * | 2005-03-16 | 2008-10-09 | ユニバーシティ オブ シカゴ | Micro fluidic system |
US8986449B2 (en) * | 2006-06-28 | 2015-03-24 | Microlytic North America Inc. | Device and a method for promoting crystallisation |
-
2009
- 2009-06-15 EP EP09763807A patent/EP2352591A4/en not_active Withdrawn
- 2009-06-15 JP JP2011513753A patent/JP2011525166A/en active Pending
- 2009-06-15 WO PCT/US2009/047414 patent/WO2009152520A2/en active Application Filing
-
2014
- 2014-05-07 JP JP2014095618A patent/JP2014240343A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5598933A (en) * | 1994-04-29 | 1997-02-04 | Phoenix International Life Sciences Inc. | Method for extraction, extraction cartridge and automated extraction processing system |
US20040115731A1 (en) * | 2001-04-06 | 2004-06-17 | California Institute Of Technology | Microfluidic protein crystallography |
US20050205005A1 (en) * | 2001-04-06 | 2005-09-22 | California Institute Of Technology | Microfluidic protein crystallography |
US20060003439A1 (en) * | 2004-07-02 | 2006-01-05 | Ismagilov Rustem F | Microfluidic system |
US20060027780A1 (en) * | 2004-07-12 | 2006-02-09 | Decode Biostructures, Inc. | Crystallization solutions |
US20060094119A1 (en) * | 2004-10-29 | 2006-05-04 | Ismagilov Rustem F | Microfluidic system |
Also Published As
Publication number | Publication date |
---|---|
EP2352591A2 (en) | 2011-08-10 |
EP2352591A4 (en) | 2013-01-23 |
JP2014240343A (en) | 2014-12-25 |
JP2011525166A (en) | 2011-09-15 |
WO2009152520A2 (en) | 2009-12-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2009152520A3 (en) | Nanovolume microcapillary crystallization system | |
Adisoejoso et al. | Two-dimensional crystal engineering: A four-component architecture at a liquid-solid interface | |
Killian et al. | Mechanism of calcite co-orientation in the sea urchin tooth | |
FI3351539T3 (en) | Crystalline monohydrate complex of 1-cyano-2-(4-cyclopropyl-benzyl)-4-(beta-d-glucopyranos-1-yl)-benzene and l-proline in crystal water (1:1:1), methods for its preparation and its use as an sglt inhibitor | |
TWI349029B (en) | Liquid crystalline medium, liquid crystal display panel using the same, and method for manufacturing liquid crystal display panel | |
WO2013095282A3 (en) | Quantitative phase microscopy for label-free high-contrast cell imaging | |
GB201300396D0 (en) | Systems and methods for harvesting and/or analyzing biological samples | |
Olsen et al. | Pressure-induced phase transformations in l-alanine crystals | |
WO2010147625A3 (en) | Systems and methods for determining process conditions in confined volumes | |
Rovira et al. | Crystal engineering in molecular magnetism | |
EP2267193A4 (en) | Process for producing zno single crystal, self-supporting zno single-crystal wafer obtained by the same, self-supporting wafer of mg-containing zno mixed single crystal, and process for producing mg-containing zno mixed single crystal for use in the same | |
WO2012142541A8 (en) | Single molecule imaging techniques to aid crystallization | |
WO2009115664A3 (en) | Crystalline structure of the cc2-lz domain of nemo | |
Gartrell et al. | Variable inversion of polyphase rift basins impacts the Triassic sequence architecture of the NW Shelf, Australia | |
李文明 et al. | Syntheses and Crystal Structures of (S)-Methyl 2-(4-R-phenylsulfonamido)-3-(1H-indol-3-yl) propanoate (R= H (1) and Cl (2)) | |
郭万成 et al. | Synthesis and Crystal Structure of 2-Amino-4-chloro-5-(4-methylbenzyl)-6-methylpyrimidine | |
李夜雨 et al. | Synthesis, Crystal and Electronic Structure of Ba_3ZnSb_2O_9 with the 6H-perovskite-type Structure | |
Tashkhodjaev et al. | The Crystal Structural Variety of Tricyclic Quinazoline Salts | |
Gonzalez-DeWhitt | Measure and Might of the MiTeGen In Situ-1™ Crystallization Plate for Microgravity Protein Crystal Growth | |
Lamia | Crystal Structure of a New Hybrid Cyclohexaphosphate,[C8H12N] 4Li2P6O18 4H2O | |
Thornea et al. | Ice Formation and Solvent Nanoconfinement in Protein Crystals | |
覃业燕 et al. | Synthesis and crystal structure of a Mo-S cluster compound coordinated by isonicotinate | |
Qu et al. | Analysis of Structural Condition and Thermodynamics Condition of Graphite Heterogeneity Nucleation in Cast Iron | |
Allaire et al. | Producing Diffraction Quality Powders from Soluble Lysozyme and Thaumatin | |
陈建新 et al. | Hydrothermal Assembly and Structural Characterization of a Novel 3D Coordination Polymer:[Cd_2Zn (BTC) _2 (H_2O) _4] _n· 2nH_2O |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 09763807 Country of ref document: EP Kind code of ref document: A2 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2011513753 Country of ref document: JP |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
REEP | Request for entry into the european phase |
Ref document number: 2009763807 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2009763807 Country of ref document: EP |