WO2009041409A1 - クレアチニンアミドヒドロラーゼ改変体 - Google Patents
クレアチニンアミドヒドロラーゼ改変体 Download PDFInfo
- Publication number
- WO2009041409A1 WO2009041409A1 PCT/JP2008/067145 JP2008067145W WO2009041409A1 WO 2009041409 A1 WO2009041409 A1 WO 2009041409A1 JP 2008067145 W JP2008067145 W JP 2008067145W WO 2009041409 A1 WO2009041409 A1 WO 2009041409A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- amino acid
- acid residue
- creatinine amidohydrolase
- residue located
- amidohydrolase
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
【課題】公知のクレアチニンアミドヒドロラーゼの欠点を克服し、よりキレート剤に対する耐性が向上した、あるいは、より比活性が向上したクレアチニンアミドヒドロラーゼを提供すること。 【解決手段】シュードモナス・プチダ由来クレアチニンアミドヒドロラーゼのアミノ酸配列の175位、124位、78位、122位のうちのいずれか1つ以上、あるいはそれと同等の位置のアミノ酸を他のアミノ酸に置換することを特徴とするクレアチニンアミドヒドロラーゼのキレート剤耐性を向上させる方法、もしくは、43位、あるいはそれと同等の位置のアミノ酸を他のアミノ酸に置換することを特徴とするクレアチニンアミドヒドロラーゼの比活性を向上させる方法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP08833630.0A EP2202304B1 (en) | 2007-09-27 | 2008-09-24 | Modified creatinine amidohydrolase |
Applications Claiming Priority (10)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007250874A JP5130479B2 (ja) | 2007-09-27 | 2007-09-27 | クレアチニンアミドヒドロラーゼの比活性を向上させる方法 |
JP2007250876A JP2009077682A (ja) | 2007-09-27 | 2007-09-27 | キレート剤耐性が向上したクレアチニンアミドヒドロラーゼ改変体 |
JP2007-250875 | 2007-09-27 | ||
JP2007-250878 | 2007-09-27 | ||
JP2007-250876 | 2007-09-27 | ||
JP2007250878A JP2009077684A (ja) | 2007-09-27 | 2007-09-27 | キレート剤耐性が向上したクレアチニンアミドヒドロラーゼ改変体 |
JP2007250875A JP5130480B2 (ja) | 2007-09-27 | 2007-09-27 | キレート剤耐性が向上したクレアチニンアミドヒドロラーゼ改変体 |
JP2007250877A JP2009077683A (ja) | 2007-09-27 | 2007-09-27 | キレート剤耐性が向上したクレアチニンアミドヒドロラーゼ改変体 |
JP2007-250874 | 2007-09-27 | ||
JP2007-250877 | 2007-09-27 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2009041409A1 true WO2009041409A1 (ja) | 2009-04-02 |
Family
ID=40511294
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2008/067145 WO2009041409A1 (ja) | 2007-09-27 | 2008-09-24 | クレアチニンアミドヒドロラーゼ改変体 |
Country Status (2)
Country | Link |
---|---|
EP (1) | EP2202304B1 (ja) |
WO (1) | WO2009041409A1 (ja) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102253041B (zh) * | 2011-06-20 | 2013-02-20 | 董理 | 一种检测肌酐的试剂盒 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS51115989A (en) | 1975-04-05 | 1976-10-13 | Noda Sangyo Kagaku Kenkyusho | Process for preparing creatinine amide hydrolase |
JP2527035B2 (ja) | 1989-06-16 | 1996-08-21 | 東洋紡績株式会社 | クレアチニン・アミドヒドロラ―ゼをコ―ドする遺伝子を含有するdna断片、該dna断片を有する組換えベクタ―及び該組換えベクタ―を有する形質転換体、並びにクレアチニン・アミドヒドロラ―ゼの製造方法 |
JPH09154574A (ja) | 1995-12-01 | 1997-06-17 | Toyobo Co Ltd | 新規なクレアチニンアミドヒドロラーゼ |
JP2006303451A (ja) | 2005-03-23 | 2006-11-02 | Renesas Technology Corp | 半導体装置及び半導体装置の製造方法 |
JP2008136477A (ja) * | 2006-04-25 | 2008-06-19 | Toyobo Co Ltd | 基質との親和性が向上したクレアチニンアミドヒドロラーゼ改変体、および、クレアチニン測定用試薬組成物 |
-
2008
- 2008-09-24 WO PCT/JP2008/067145 patent/WO2009041409A1/ja active Application Filing
- 2008-09-24 EP EP08833630.0A patent/EP2202304B1/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS51115989A (en) | 1975-04-05 | 1976-10-13 | Noda Sangyo Kagaku Kenkyusho | Process for preparing creatinine amide hydrolase |
JP2527035B2 (ja) | 1989-06-16 | 1996-08-21 | 東洋紡績株式会社 | クレアチニン・アミドヒドロラ―ゼをコ―ドする遺伝子を含有するdna断片、該dna断片を有する組換えベクタ―及び該組換えベクタ―を有する形質転換体、並びにクレアチニン・アミドヒドロラ―ゼの製造方法 |
JPH09154574A (ja) | 1995-12-01 | 1997-06-17 | Toyobo Co Ltd | 新規なクレアチニンアミドヒドロラーゼ |
JP2006303451A (ja) | 2005-03-23 | 2006-11-02 | Renesas Technology Corp | 半導体装置及び半導体装置の製造方法 |
JP2008136477A (ja) * | 2006-04-25 | 2008-06-19 | Toyobo Co Ltd | 基質との親和性が向上したクレアチニンアミドヒドロラーゼ改変体、および、クレアチニン測定用試薬組成物 |
Non-Patent Citations (8)
Title |
---|
BEUTH, B. ET AL.: "Crystal structure of creatininase from Pseudomonas putida: a novel fold and a case of convergent evolution.", J MOL BIOL., vol. 332, 2003, pages 287 - 301, XP002605326 * |
BIOSCI. BIOTECH. BIOCHEM., vol. 59, no. 7, 1995, pages 1331 - 1332 |
CHEMICAL AND PHARMACEUTICAL BULLETIN, vol. 34, no. 1, 1986, pages 269 - 274 |
JOURNAL OF BIOCHEMISTRY, vol. 86, 1979, pages 1109 - 1117 |
JOURNAL OF MOLECULAR BIOLOGY, vol. 332, 2004, pages 287 - 301 |
JOURNAL OF MOLECULAR BIOLOGY, vol. 337, 2004, pages 399 - 416 |
SCIENCE, vol. 214, 1981, pages 1205 |
See also references of EP2202304A4 * |
Also Published As
Publication number | Publication date |
---|---|
EP2202304A1 (en) | 2010-06-30 |
EP2202304B1 (en) | 2016-08-17 |
EP2202304A4 (en) | 2010-11-24 |
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