WO2009039862A3 - Enzymatic incorporation of lna nucleotides - Google Patents

Enzymatic incorporation of lna nucleotides Download PDF

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Publication number
WO2009039862A3
WO2009039862A3 PCT/DK2008/050240 DK2008050240W WO2009039862A3 WO 2009039862 A3 WO2009039862 A3 WO 2009039862A3 DK 2008050240 W DK2008050240 W DK 2008050240W WO 2009039862 A3 WO2009039862 A3 WO 2009039862A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligonucleotides
lna nucleotides
enzymatic incorporation
lna units
methods
Prior art date
Application number
PCT/DK2008/050240
Other languages
French (fr)
Other versions
WO2009039862A2 (en
Inventor
Jesper Wengel
Birte Vester
Rakesh N Veedu
Original Assignee
Ribotask Aps
Jesper Wengel
Birte Vester
Rakesh N Veedu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ribotask Aps, Jesper Wengel, Birte Vester, Rakesh N Veedu filed Critical Ribotask Aps
Publication of WO2009039862A2 publication Critical patent/WO2009039862A2/en
Publication of WO2009039862A3 publication Critical patent/WO2009039862A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to oligonucleotides comprising LNA units and enzymatic methods of synthesizing such oligonucleotides. Oligonucleotide comprising LNA units have various characteristics, e.g. improved biostability and increased affinity for complementary oligonucleotides. The methods may be part of detection reactions, polymerase chain reaction, RNA transcription or in vitro evolution processes such as SELEX.
PCT/DK2008/050240 2007-09-28 2008-09-26 Enzymatic incorporation of lna nucleotides WO2009039862A2 (en)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US97581807P 2007-09-28 2007-09-28
US60/975,818 2007-09-28
DKPA200701409 2007-09-28
DKPA200701409 2007-09-28
DKPA200701626 2007-11-15
DKPA200701626 2007-11-15
DKPA200801038 2008-07-25
DKPA200801038 2008-07-25

Publications (2)

Publication Number Publication Date
WO2009039862A2 WO2009039862A2 (en) 2009-04-02
WO2009039862A3 true WO2009039862A3 (en) 2009-05-28

Family

ID=40134729

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2008/050240 WO2009039862A2 (en) 2007-09-28 2008-09-26 Enzymatic incorporation of lna nucleotides

Country Status (1)

Country Link
WO (1) WO2009039862A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170029879A1 (en) * 2014-02-07 2017-02-02 Ibis Biosciences, Inc. Improved sensitivity for molecular detection of microbes in the bloodstream
US9688707B2 (en) * 2014-12-30 2017-06-27 Ionis Pharmaceuticals, Inc. Bicyclic morpholino compounds and oligomeric compounds prepared therefrom
CN107466323A (en) 2015-02-15 2017-12-12 阿克丘勒斯治疗公司 Acyl amino LNA and/or hydrocarbylamino LNA oligonucleotides

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020068708A1 (en) * 1997-09-12 2002-06-06 Jesper Wengel Oligonucleotide analogues

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020068708A1 (en) * 1997-09-12 2002-06-06 Jesper Wengel Oligonucleotide analogues

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
VEEDU RAKESH N ET AL: "Enzymatic incorporation of LNA nucleotides into DNA strands.", CHEMBIOCHEM : A EUROPEAN JOURNAL OF CHEMICAL BIOLOGY 26 MAR 2007, vol. 8, no. 5, 26 March 2007 (2007-03-26), pages 490 - 492, XP002509043, ISSN: 1439-4227 *

Also Published As

Publication number Publication date
WO2009039862A2 (en) 2009-04-02

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