IN2014DN07878A - - Google Patents
Info
- Publication number
- IN2014DN07878A IN2014DN07878A IN7878DEN2014A IN2014DN07878A IN 2014DN07878 A IN2014DN07878 A IN 2014DN07878A IN 7878DEN2014 A IN7878DEN2014 A IN 7878DEN2014A IN 2014DN07878 A IN2014DN07878 A IN 2014DN07878A
- Authority
- IN
- India
- Prior art keywords
- relates
- methods
- primer extension
- polymerase lacking
- detection
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Abstract
The present invention relates to methods and kits for nucleic acid detection in an assay system. In one aspect the invention relates to a method for reducing non specific amplification and primer dimer formation in a primer extension reaction in the presence of a polymerase lacking 3'-5' nuclease activity. In another aspect the invention relates to a method for the detection of a primer extension product produced in the presence of a polymerase lacking 3'-5' exonuclease activity comprising providing a first and second single labelled oligonucleotide of differing Tm able to hybridise to one another to form a fluorescent quenched pair. In the methods of the invention one or more of the primers or oligonucleotides contains at least one phosphorothioate group.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/GB2012/050645 WO2013140107A1 (en) | 2012-03-22 | 2012-03-22 | Polymerase chain reaction detection system using oligonucleotides comprising a phosphorothioate group |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IN2014DN07878A true IN2014DN07878A (en) | 2015-04-24 |
Family
ID=45894595
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IN7878DEN2014 IN2014DN07878A (en) | 2012-03-22 | 2012-03-22 |
Country Status (19)
| Country | Link |
|---|---|
| EP (1) | EP2828399B1 (en) |
| JP (1) | JP6166342B2 (en) |
| CN (1) | CN104379763B (en) |
| AU (1) | AU2012374566B2 (en) |
| CA (1) | CA2867916C (en) |
| CY (1) | CY1118903T1 (en) |
| DK (1) | DK2828399T3 (en) |
| ES (1) | ES2620750T3 (en) |
| HK (1) | HK1204015A1 (en) |
| HR (1) | HRP20170569T1 (en) |
| HU (1) | HUE032212T2 (en) |
| IN (1) | IN2014DN07878A (en) |
| LT (1) | LT2828399T (en) |
| PL (1) | PL2828399T3 (en) |
| PT (1) | PT2828399T (en) |
| RS (1) | RS55868B1 (en) |
| SG (1) | SG11201502154UA (en) |
| SI (1) | SI2828399T1 (en) |
| WO (1) | WO2013140107A1 (en) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2515990B (en) * | 2013-03-06 | 2016-05-04 | Lgc Genomics Ltd | Polymerase chain reaction detection system |
| CN104560972A (en) * | 2014-11-28 | 2015-04-29 | 深圳市海普洛斯生物科技有限公司 | PCR (Polymerase chain reaction) primer and modification method thereof as well as parallel high-flux single molecule high-fidelity amplification method |
| EP3250716B1 (en) | 2015-01-30 | 2021-07-07 | President and Fellows of Harvard College | Microscope-free imaging |
| JP7085999B2 (en) | 2016-02-17 | 2022-06-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Molecular programming tool |
| CN109923213B (en) * | 2016-09-20 | 2023-02-28 | 哈佛学院院长及董事 | Molecular verification system |
| CN106755379B (en) * | 2016-12-13 | 2021-06-04 | 海南医学院 | Dimer mutation fluorescent primer quantitative PCR method for synchronously quantifying and genotyping 4 aspergillus |
| RU2698134C2 (en) | 2017-12-04 | 2019-08-22 | Общество с ограниченной ответственностью "Биолабмикс" | Method for amplification of nucleic acids using phosphoryl-guanidine oligonucleotides |
| GB201713251D0 (en) * | 2017-08-18 | 2017-10-04 | Lgc Genomics Ltd | Methods and kits for detection of nucleic acid molecules |
| KR102380264B1 (en) * | 2017-08-31 | 2022-03-29 | 주식회사 씨젠 | Evaluation of the performance of components using dimer-forming primer pairs |
| US11753681B2 (en) * | 2017-11-03 | 2023-09-12 | University Of Washington | Digital nucleic acid amplification using encoded particles |
| CN108715888A (en) * | 2018-06-07 | 2018-10-30 | 王卫东 | A kind of homogeneous detecting systems of PCR based on FRET and its application |
| CN112342213B (en) * | 2020-10-26 | 2022-10-11 | 清华大学 | Nucleic acid molecules and uses thereof |
| CN113846187B (en) * | 2021-10-24 | 2023-07-18 | 北京宏微特斯生物科技有限公司 | Method for detecting target nucleic acid sequence to be detected by utilizing fluorescent probe melting curve and kit thereof |
| CN114250281A (en) * | 2021-11-03 | 2022-03-29 | 深圳铭毅智造科技有限公司 | Method for detecting activity of nucleic acid metabolic enzyme |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5516633A (en) * | 1991-08-15 | 1996-05-14 | Amersham Life Science, Inc. | DNA sequencing with a T7-type gene 6 exonuclease |
| CA2176193A1 (en) * | 1995-05-22 | 1996-11-23 | John Wesley Backus | Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers |
| US6727356B1 (en) | 1999-12-08 | 2004-04-27 | Epoch Pharmaceuticals, Inc. | Fluorescent quenching detection reagents and methods |
| US7019129B1 (en) | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
| GB0510979D0 (en) | 2005-05-28 | 2005-07-06 | Kbiosciences Ltd | Detection system for PCR assay |
| AT502823B1 (en) * | 2005-11-29 | 2007-06-15 | Seitz Alexander Dr | POLYNUCLEOTIDE AMPLIFICATION |
-
2012
- 2012-03-22 CA CA2867916A patent/CA2867916C/en active Active
- 2012-03-22 PL PL12710994T patent/PL2828399T3/en unknown
- 2012-03-22 IN IN7878DEN2014 patent/IN2014DN07878A/en unknown
- 2012-03-22 ES ES12710994.0T patent/ES2620750T3/en active Active
- 2012-03-22 PT PT127109940T patent/PT2828399T/en unknown
- 2012-03-22 SG SG11201502154UA patent/SG11201502154UA/en unknown
- 2012-03-22 HU HUE12710994A patent/HUE032212T2/en unknown
- 2012-03-22 LT LTEP12710994.0T patent/LT2828399T/en unknown
- 2012-03-22 RS RS20170376A patent/RS55868B1/en unknown
- 2012-03-22 AU AU2012374566A patent/AU2012374566B2/en active Active
- 2012-03-22 JP JP2015500972A patent/JP6166342B2/en active Active
- 2012-03-22 SI SI201230926A patent/SI2828399T1/en unknown
- 2012-03-22 EP EP12710994.0A patent/EP2828399B1/en active Active
- 2012-03-22 CN CN201280073407.6A patent/CN104379763B/en active Active
- 2012-03-22 WO PCT/GB2012/050645 patent/WO2013140107A1/en active Application Filing
- 2012-03-22 DK DK12710994.0T patent/DK2828399T3/en active
-
2015
- 2015-05-07 HK HK15104355.4A patent/HK1204015A1/en unknown
-
2017
- 2017-04-10 HR HRP20170569TT patent/HRP20170569T1/en unknown
- 2017-04-13 CY CY20171100441T patent/CY1118903T1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP2828399A1 (en) | 2015-01-28 |
| NZ630915A (en) | 2016-08-26 |
| DK2828399T3 (en) | 2017-04-10 |
| EP2828399B1 (en) | 2017-01-18 |
| AU2012374566A2 (en) | 2015-12-24 |
| SG11201502154UA (en) | 2015-05-28 |
| SI2828399T1 (en) | 2017-05-31 |
| CN104379763B (en) | 2017-04-19 |
| CN104379763A (en) | 2015-02-25 |
| JP2015512251A (en) | 2015-04-27 |
| RS55868B1 (en) | 2017-08-31 |
| HK1204015A1 (en) | 2015-11-06 |
| LT2828399T (en) | 2017-04-10 |
| PT2828399T (en) | 2017-04-04 |
| CA2867916A1 (en) | 2013-09-26 |
| AU2012374566B2 (en) | 2018-05-10 |
| CA2867916C (en) | 2020-05-26 |
| JP6166342B2 (en) | 2017-07-19 |
| HRP20170569T1 (en) | 2017-06-30 |
| ES2620750T3 (en) | 2017-06-29 |
| WO2013140107A1 (en) | 2013-09-26 |
| AU2012374566A1 (en) | 2014-10-02 |
| HUE032212T2 (en) | 2017-09-28 |
| PL2828399T3 (en) | 2017-07-31 |
| CY1118903T1 (en) | 2018-01-10 |
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