WO2009025361A1 - 電気泳動用マイクロ流路チップ及び電気泳動方法 - Google Patents

電気泳動用マイクロ流路チップ及び電気泳動方法 Download PDF

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Publication number
WO2009025361A1
WO2009025361A1 PCT/JP2008/065024 JP2008065024W WO2009025361A1 WO 2009025361 A1 WO2009025361 A1 WO 2009025361A1 JP 2008065024 W JP2008065024 W JP 2008065024W WO 2009025361 A1 WO2009025361 A1 WO 2009025361A1
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WO
WIPO (PCT)
Prior art keywords
electrophoresis
channel
micro
substrate
channel chip
Prior art date
Application number
PCT/JP2008/065024
Other languages
English (en)
French (fr)
Inventor
Yoshinori Mishina
Hisashi Hagiwara
Original Assignee
Aida Engineering, Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aida Engineering, Ltd. filed Critical Aida Engineering, Ltd.
Priority to EP08827833A priority Critical patent/EP2184602A4/en
Priority to CA2696971A priority patent/CA2696971A1/en
Priority to US12/674,481 priority patent/US20110120867A1/en
Publication of WO2009025361A1 publication Critical patent/WO2009025361A1/ja

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Dispersion Chemistry (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

　本発明の電気泳動用マイクロ流路チップは、気体透過性の材料から形成された第１の基板と、気体透過性又は気体不透過性の材料から形成された第２の基板とからなり、前記第１の基板と第２の基板とは相互に貼り合わされており、前記第１の基板及び第２の基板の何れか一方の貼り合わせ面側に、両端にポートを有するサンプル送入用チャネルと、両端にポートを有する電気泳動用チャネルとを有し、前記サンプル送入用チャネルと電気泳動用チャネルとは、これらのチャネルの断面積よりも小さな断面積を有する狭小チャネルにより連通されている。本発明の電気泳動用マイクロ流路チップによれば、電気泳動用の電源が１個だけで済み、かつ、サンプル量の無駄を極力少なくすることができる
PCT/JP2008/065024 2007-08-22 2008-08-22 電気泳動用マイクロ流路チップ及び電気泳動方法 WO2009025361A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP08827833A EP2184602A4 (en) 2007-08-22 2008-08-22 MICROCHANNEL CHIP FOR ELECTROPHORESIS AND METHOD FOR ELECTROPHORESIS
CA2696971A CA2696971A1 (en) 2007-08-22 2008-08-22 Micro-channel chip for electrophoresis and method for electrophoresis
US12/674,481 US20110120867A1 (en) 2007-08-22 2008-08-22 Micro-channel chip for electrophoresis and method for electrophoresis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-215665 2007-08-22
JP2007215665A JP5052996B2 (ja) 2007-08-22 2007-08-22 電気泳動用マイクロ流路チップ及び電気泳動方法

Publications (1)

Publication Number Publication Date
WO2009025361A1 true WO2009025361A1 (ja) 2009-02-26

Family

ID=40378261

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/065024 WO2009025361A1 (ja) 2007-08-22 2008-08-22 電気泳動用マイクロ流路チップ及び電気泳動方法

Country Status (5)

Country Link
US (1) US20110120867A1 (ja)
EP (1) EP2184602A4 (ja)
JP (1) JP5052996B2 (ja)
CA (1) CA2696971A1 (ja)
WO (1) WO2009025361A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130175170A1 (en) * 2012-01-09 2013-07-11 Washington State University Research Foundation Electrophoresis systems, devices, and associated methods of analysis

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6003772B2 (ja) * 2013-03-29 2016-10-05 ソニー株式会社 マイクロチップ及びマイクロチップの製造方法
CN103558393B (zh) * 2013-10-24 2015-07-29 山东大学 用于血液样品中痕量靶标物质的检测装置
KR102195769B1 (ko) * 2014-07-10 2020-12-30 주식회사 미코바이오메드 미세유체 칩, 이의 제조 방법 및 이를 이용한 분석 장치
US10734216B2 (en) 2015-05-11 2020-08-04 The University Of North Carolina At Chapel Hill Pressure driven fluidic injection for chemical separations by electrophoresis
US9606082B2 (en) 2015-05-11 2017-03-28 The University Of North Carolina At Chapel Hill Pressure driven microfluidic injection for chemical separations
US9728387B2 (en) 2015-10-20 2017-08-08 The University Of North Carolina At Chapel Hill Solid phase extraction with capillary electrophoresis
US10564121B2 (en) * 2015-11-26 2020-02-18 Vladislav Dolnik Device and method for separation and analysis of trace and ultra-trace ionogenic compounds by isotachophoresis and zone electrophoresis on chip
WO2018065985A1 (en) 2016-10-06 2018-04-12 Moran Bercovici Device and method for isotachophoretic focusing large sample volumes
JP6983089B2 (ja) * 2018-03-12 2021-12-17 富士フイルム和光純薬株式会社 マイクロ流路チップ及びその製造方法
JP7173759B2 (ja) * 2018-05-29 2022-11-16 株式会社フコク マイクロ流路チップ
JP7057728B2 (ja) * 2018-07-13 2022-04-20 浜松ホトニクス株式会社 電気泳動方法、電気泳動システム、及び電気泳動用の収容容器
JP7404639B2 (ja) * 2019-04-01 2023-12-26 東ソー株式会社 流路構造体への流体充填方法
CN112354570B (zh) * 2019-07-11 2022-02-11 北京理工大学 一种多维微流控电泳芯片及检测装置，检测方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6321556A (ja) 1986-07-15 1988-01-29 Hitachi Ltd Ｄｎａ塩基配列決定装置
JPH11183437A (ja) 1997-12-22 1999-07-09 Shimadzu Corp 電気泳動部材およびそれを用いた電気泳動装置
JP2004151041A (ja) * 2002-11-01 2004-05-27 Asti Corp バイオチップとバイオチップ製造方法
JP2004163104A (ja) * 2001-10-18 2004-06-10 Aida Eng Ltd 微量液体秤取構造及び該構造を有するマイクロチップ
JP2005274512A (ja) * 2004-03-26 2005-10-06 Aida Eng Ltd 電気泳動用マイクロチップ

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US6685809B1 (en) * 1999-02-04 2004-02-03 Ut-Battelle, Llc Methods for forming small-volume electrical contacts and material manipulations with fluidic microchannels
AU2001290879A1 (en) * 2000-09-15 2002-03-26 California Institute Of Technology Microfabricated crossflow devices and methods
AU2002306486A1 (en) * 2001-02-09 2002-08-28 Microchem Solutions Method and apparatus for sample injection in microfabricated devices
JP4255891B2 (ja) * 2001-10-18 2009-04-15 三菱化学株式会社 タンパク質の結晶化方法
ATE271919T1 (de) * 2001-10-18 2004-08-15 Aida Eng Ltd Mikrodosier- und probennahmevorrichtung sowie mikrochip mit dieser vorrichtung
US7799553B2 (en) * 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6321556A (ja) 1986-07-15 1988-01-29 Hitachi Ltd Ｄｎａ塩基配列決定装置
JPH11183437A (ja) 1997-12-22 1999-07-09 Shimadzu Corp 電気泳動部材およびそれを用いた電気泳動装置
JP2004163104A (ja) * 2001-10-18 2004-06-10 Aida Eng Ltd 微量液体秤取構造及び該構造を有するマイクロチップ
JP2004151041A (ja) * 2002-11-01 2004-05-27 Asti Corp バイオチップとバイオチップ製造方法
JP2005274512A (ja) * 2004-03-26 2005-10-06 Aida Eng Ltd 電気泳動用マイクロチップ

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2184602A4 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130175170A1 (en) * 2012-01-09 2013-07-11 Washington State University Research Foundation Electrophoresis systems, devices, and associated methods of analysis
US9855684B2 (en) * 2012-01-09 2018-01-02 Washington State University Electrophoresis systems, devices, and associated methods of analysis

Also Published As

Publication number Publication date
US20110120867A1 (en) 2011-05-26
JP2009047626A (ja) 2009-03-05
EP2184602A1 (en) 2010-05-12
JP5052996B2 (ja) 2012-10-17
CA2696971A1 (en) 2009-02-26
EP2184602A4 (en) 2011-04-27

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