WO2009002334A1 - Dispositif microfluidique avec scellement élastomère - Google Patents

Dispositif microfluidique avec scellement élastomère Download PDF

Info

Publication number
WO2009002334A1
WO2009002334A1 PCT/US2007/066319 US2007066319W WO2009002334A1 WO 2009002334 A1 WO2009002334 A1 WO 2009002334A1 US 2007066319 W US2007066319 W US 2007066319W WO 2009002334 A1 WO2009002334 A1 WO 2009002334A1
Authority
WO
WIPO (PCT)
Prior art keywords
fluid
microfluidic device
transmission means
microfluidic
elastomeric
Prior art date
Application number
PCT/US2007/066319
Other languages
English (en)
Inventor
Dominick Danna
Original Assignee
Welch Allyn, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Welch Allyn, Inc. filed Critical Welch Allyn, Inc.
Priority to PCT/US2007/066319 priority Critical patent/WO2009002334A1/fr
Publication of WO2009002334A1 publication Critical patent/WO2009002334A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K15/00—Check valves
    • F16K15/18—Check valves with actuating mechanism; Combined check valves and actuated valves
    • F16K15/184—Combined check valves and actuated valves
    • F16K15/1848—Check valves combined with valves having a rotating tap or cock
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00801—Means to assemble
    • B01J2219/0081—Plurality of modules
    • B01J2219/00813—Fluidic connections
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0672—Integrated piercing tool
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions

Definitions

  • This invention relates to microfluidic devices, and more particularly, to a device and method for communicating fluids and reagents to a disposable diagnostic microfluidic device while ensuring a substantially hermetic seal.
  • Microfluidic devices are used to perform a variety of analytical testing.
  • Microfluidic devices generally consist of a substrate which is constructed in a multi-layer laminated, plastic, or elastic structure, where each layer has channels fabricated to form microscale voids or channels for fluid flow. This is accomplished by photolithography, wet chemical etching, or other techniques known in the art.
  • An example of a point-of-care diagnostic microfluidic device is disclosed in United States Patent Application Nos. 10/981,369 and 11/267,647, which are hereby incorporated by reference.
  • a microscale channel is a fluid passage wherein fluid control is affected by either external pressurized fluid forced into the channels, or by structures within the device.
  • the synthetic and analytical capabilities of microfluidic devices are generally enhanced by increasing the number and complexity of network channels, reaction chambers, etc..
  • a microscale channel is generally defined as a fluid passage that has at least one internal cross-sectional dimension that is less than 500 ⁇ m and is typically between 0.1 ⁇ m and about 500 ⁇ m.
  • microfluidic devices must frequently interact with external devices or assemblies external to the microfluidic device.
  • a microfluidic device may require a connection to a larger fluid source or integration with a pump or other external assembly.
  • Fluid introduction ports e.g., fluid inlets or orifices
  • Methods and techniques limiting contamination of the microfluidic channels, and exposure of the microfluidic fluids to users and patients are currently inadequate.
  • One approach to this integration problem involves securing discrete, functional components, such as flow connectors, to a microfluidic device using an adhesive, as discussed by United States Patent Application Publication Number 2005/0151371 Al, issued to Simmons, et al..
  • This technique is subject to leakage between the microfluidic device and external components. Moreover, alignment errors may occur as each component is independently adhered to the microfluidic device.
  • Simmons et. al leaches a method of injection molding to form microfluidic devices with integrated functional components. This technique, however, does not allow for the introduction of fluids into the microfluidic device from external sources, where the sources remain external to the microfluidic device at all times.
  • microfluidic device that is sealed substantially hermetically with all contents remaining internal to the microfluidic device after use that also produces no contamination or exposure of the contents to users and patients is desired.
  • the invention comprises, in one form thereof, a low cost method of substantially hermetically sealing and resealing a microfluidic device before, during, and after communication of fluids into the microfluidic device.
  • the invention includes a microfluidic device with fluid introduction ports substantially hermetically sealed with elastomeric plugs, wherein the plugs can be penetrated by hollow needles to introduce fluids such as air, water, ethanol, or other chemical or biological reagents into the device while maintaining a substantially hermetic seal.
  • the invention includes a method for introducing fluids into a microfluidic device from an external source.
  • the method comprises the steps of penetrating fluid introduction ports substantially hermetically sealed with elastomeric plugs with hollow needles connected to an external fluid source, pumping fluid into the microfluidic device, removing the hollow needle from the plug, and resealing the plug to produce a substantially hermetic seal.
  • An advantage of the present invention is that fluids and reagents may be communicated to the microfluidic device while maintaining a substantially hermetic seal.
  • a further advantage of the present invention is that fluids such as ethanol, water, air, or other chemical or biological reagents, may be communicated to the microfluidic device from a source remains at all limes external to the microfluidic device.
  • microfluidic device retains its contents and produces substantially no contamination or exposure to patients or users.
  • elastomeric plugs need very little space on the microfluidic device and therefore can be located on the microfluidic device's edge.
  • Fig. 1 is a top perspective view of a microfluidic device according to the present invention
  • Fig. 2 is a top perspective view of an external source of fluid container communicating water, ethanol, and air into the microfluidic device according the present invention
  • Fig. 3 is a side perspective view of a microfluidic device according to the present invention.
  • the micro fluidic device 10 comprises a body structure 20 comprising a substrate.
  • the micro fluidic device is in the form of a self-contained card-like point-of-care diagnostic device, which is well known in the art.
  • At least one microfiuidic channel 30 is disposed within said substrate.
  • the term microfJuidic channel generally refers to a solution flow path into which samples can be introduced and transported.
  • the microfiuidic channel may form a loop for continuous and cyclic solution flow through the channel.
  • Other embodiments may comprise a plurality of intersecting channels to form an array or matrix of chambers or junctions at which reactions can occur.
  • microfiuidic device may contain various other components common to microfiuidic devices, such as multiple flow channels, control channels, valves and/or pumps (not shown).
  • Fluid introduction ports 40, 50, and 60 are disposed on the surface of said body structure 20.
  • the fluid introduction ports 40, 50, and 60 are in fluid communication with at least one microfiuidic channel 30 at one end, and are exposed to the environment outside the microfiuidic device on the other.
  • a collar may be molded on the microfiuidic device at the portion of each fluid introduction port exposed to the environment, as seen in Figs. 1, 2, and 3.
  • the introduction ports 40, 50, and 60 are substantially hermetically sealed with elastomeric plugs 70, 80, and 90.
  • the said collar captures and holds said elastomeric plugs.
  • the term "elastomer” and "elastomeric” has its general meaning as used in the art, e.g., as polymers existing at a temperature between their glass transition temperature and liquefaction temperature. Elastomeric materials exhibit elastic properties because the polymer chains readily undergo torsional motion to permit uncoiling of the backbone chains in response to a force, with the backbone chains recoiling to assume the prior shape in the absence of the force. Elastomers deform when force is applied, but then return to their original shape when the force is removed. Elastomeric plugs 70, 80, and 90 are penetrable by a fluid transmission means.
  • fluid transmission means are shown as hollow needles
  • the fluid transmission means 100, 110, and 120 are in fluid communication with an fluid source 130 located external to the microfiuidic device 10.
  • the external fluid source 130 may contain reservoirs for storing fluids such as ethanol 140 or water 150, or may be comprised of an air pump 160. Fluids are communicated from the external fluid source 130 to the microfmidic device 10 by penetrating at least one fluid introduction port 40, 50, or 60 of the microfluidic device 10 with at least one fluid communication means 100, 110, or 120.
  • communication is accomplished by causing the hollow needles 100, 110, and 120 to penetrate the elastomeric plugs 70, 80, and 90, and transmiting fluid from the external fluid source 130 through the fluid introduction ports 40, 50, and 60 to the microfluidic channel 30 disposed inside the microfluidic device 10.
  • the elastomeric plugs 70, 80, and 90 maintain substantially hermetic seals before, during, and after penetration by the needles 100, 110, and 120.
  • the needles 100, 110, and 120 are then removed from contact with the elastomeric plugs 70, 80, and 90.
  • the elastomeric plugs 70, 80, and 90 self-reseal to form a substantially hermetic seal following penetration.
  • the microfluidic device 10 is comprised of an upper surface 200 and a lower surface 210 connected by an edge.
  • fluid introduction ports 40, 50, and 60 are disposed in this edge, however, they may be disposed on any surface of the microfluidic device 10 as needed.
  • Fluid introduction ports 40, 50, and 60 are substantially hermetically sealed by elastomeric plugs 70, 80, and 90.
  • Elastomeric plugs 70, 80, and 90 are sized such that a substantially hermetic seal is maintained at all times in fluid introduction ports 40, 50, and 60.
  • the elastomeric plugs prevent leakage and exposure to users and patients by containing all solutions and samples communicated to the device and concurrently prevent contamination of the device's contents by external contaminants.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Mechanical Engineering (AREA)
  • Organic Chemistry (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne un procédé à faible coût de décalaminage et de rescellement étanche d'un dispositif microfluidique avant, pendant et après une communication de fluides dans le dispositif microfluidique. L'invention concerne un dispositif microfluidique ayant des orifices d'introduction de fluide scellés de façon sensiblement étanche par des bouchons élastomères, les bouchons pouvant être pénétrés par des aiguilles creuses pour introduire des fluides tels que de l'air, de l'eau, de l'éthanol ou autres réactifs chimiques ou biologiques, dans le dispositif tout en maintenant un scellement sensiblement étanche. L'invention concerne un procédé pour introduire des fluides dans un dispositif microfluidique à partir d'une source externe. Le procédé comprend les étapes consistant à faire pénétrer des aiguilles creuses, reliées à une source de fluide, dans des orifices d'introduction de fluide scellés de façon sensiblement étanche par des bouchons élastomères, à pomper du fluide dans le dispositif et à retirer l'aiguille creuse du bouchon, après quoi le bouchon se rescelle de façon étanche de lui-même pour former un scellement sensiblement étanche.
PCT/US2007/066319 2007-06-26 2007-06-26 Dispositif microfluidique avec scellement élastomère WO2009002334A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/US2007/066319 WO2009002334A1 (fr) 2007-06-26 2007-06-26 Dispositif microfluidique avec scellement élastomère

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2007/066319 WO2009002334A1 (fr) 2007-06-26 2007-06-26 Dispositif microfluidique avec scellement élastomère

Publications (1)

Publication Number Publication Date
WO2009002334A1 true WO2009002334A1 (fr) 2008-12-31

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/066319 WO2009002334A1 (fr) 2007-06-26 2007-06-26 Dispositif microfluidique avec scellement élastomère

Country Status (1)

Country Link
WO (1) WO2009002334A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2404673A1 (fr) * 2010-07-09 2012-01-11 Syddansk Universitet Puce micro-fluide et connecteur
WO2019096925A1 (fr) * 2017-11-16 2019-05-23 Ge Healthcare Bio-Sciences Ab Procédé et système de fabrication de produit biopharmaceutiques

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306103B1 (en) * 2000-01-03 2001-10-23 Sheila L. Tyler Blood/body fluid collection apparatus and method
US20050202504A1 (en) * 1995-06-29 2005-09-15 Affymetrix, Inc. Miniaturized genetic analysis systems and methods

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050202504A1 (en) * 1995-06-29 2005-09-15 Affymetrix, Inc. Miniaturized genetic analysis systems and methods
US6306103B1 (en) * 2000-01-03 2001-10-23 Sheila L. Tyler Blood/body fluid collection apparatus and method

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2404673A1 (fr) * 2010-07-09 2012-01-11 Syddansk Universitet Puce micro-fluide et connecteur
WO2019096925A1 (fr) * 2017-11-16 2019-05-23 Ge Healthcare Bio-Sciences Ab Procédé et système de fabrication de produit biopharmaceutiques
US11103846B2 (en) 2017-11-16 2021-08-31 Cytiva Sweden Ab Method and system for manufacturing of biopharmaceutical products
US11648523B2 (en) 2017-11-16 2023-05-16 Cytiva Sweden Ab Method and system for manufacturing of biopharmaceutical products

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