WO2008087998A1 - Rsisを用いた簡便な遺伝子発現抑制方法 - Google Patents

Rsisを用いた簡便な遺伝子発現抑制方法 Download PDF

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Publication number
WO2008087998A1
WO2008087998A1 PCT/JP2008/050481 JP2008050481W WO2008087998A1 WO 2008087998 A1 WO2008087998 A1 WO 2008087998A1 JP 2008050481 W JP2008050481 W JP 2008050481W WO 2008087998 A1 WO2008087998 A1 WO 2008087998A1
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WO
WIPO (PCT)
Prior art keywords
rsis
target gene
genes
inhibition
gene expression
Prior art date
Application number
PCT/JP2008/050481
Other languages
English (en)
French (fr)
Inventor
Fumio Takaiwa
Hiroshi Yasuda
Original Assignee
National Institute Of Agrobiological Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Institute Of Agrobiological Sciences filed Critical National Institute Of Agrobiological Sciences
Priority to CA002675364A priority Critical patent/CA2675364A1/en
Priority to JP2008554067A priority patent/JP5206419B2/ja
Priority to US12/523,233 priority patent/US20100088780A1/en
Priority to AU2008205931A priority patent/AU2008205931B2/en
Priority to EP08703341A priority patent/EP2119779A4/en
Publication of WO2008087998A1 publication Critical patent/WO2008087998A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

　本発明者らはＲＳＩＳを見いだし、ＲＳＩＳを標的遺伝子のプロモーターからｍＲＮＡの５’末端配列および終止コドン以降の３’ＵＴＲを含むターミネーター配列の間に連結することによって標的遺伝子の発現を抑制することができた。また、異なる遺伝子由来のプロモーターとターミネーターおよびそれぞれのｍＲＮＡの一部を用いることによって、プロモーターの働く細胞内で両遺伝子の発現を同時に抑制することができることも示した。
PCT/JP2008/050481 2007-01-18 2008-01-17 Rsisを用いた簡便な遺伝子発現抑制方法 WO2008087998A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002675364A CA2675364A1 (en) 2007-01-18 2008-01-17 Convenient method for inhibition of gene expression using rsis
JP2008554067A JP5206419B2 (ja) 2007-01-18 2008-01-17 Ｒｓｉｓを用いた簡便な遺伝子発現抑制方法
US12/523,233 US20100088780A1 (en) 2007-01-18 2008-01-17 Convenient Method for Inhibition of Gene Expression Using RSIS
AU2008205931A AU2008205931B2 (en) 2007-01-18 2008-01-17 Convenient method for inhibition of gene expression using RSIS
EP08703341A EP2119779A4 (en) 2007-01-18 2008-01-17 USEFUL PROCEDURE FOR INHIBITING GENE EXPRESSION BY RSIS

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007008994 2007-01-18
JP2007-008994 2007-01-18

Publications (1)

Publication Number Publication Date
WO2008087998A1 true WO2008087998A1 (ja) 2008-07-24

Family

ID=39635999

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/050481 WO2008087998A1 (ja) 2007-01-18 2008-01-17 Rsisを用いた簡便な遺伝子発現抑制方法

Country Status (6)

Country Link
US (1) US20100088780A1 (ja)
EP (1) EP2119779A4 (ja)
JP (1) JP5206419B2 (ja)
AU (1) AU2008205931B2 (ja)
CA (1) CA2675364A1 (ja)
WO (1) WO2008087998A1 (ja)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004087910A1 (ja) * 2003-03-28 2004-10-14 National Institute Of Agrobiological Sciences 組換えタンパク質が高生産された植物貯蔵器官の生産方法及び新規組換えタンパク質
WO2006030492A1 (ja) * 2004-09-14 2006-03-23 Nippon Paper Industries Co.,Ltd. Ｇｌｐ−１誘導体が集積された植物及び植物貯蔵器官とその生産方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004003180A1 (en) * 2002-07-01 2004-01-08 E.I. Du Pont De Nemours And Company Method of controlling gene silencing using site-specific recombination
CN100354306C (zh) * 2002-10-11 2007-12-12 株式会社三和化学研究所 Glp－1衍生物及其经粘膜吸收的制剂

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004087910A1 (ja) * 2003-03-28 2004-10-14 National Institute Of Agrobiological Sciences 組換えタンパク質が高生産された植物貯蔵器官の生産方法及び新規組換えタンパク質
WO2006030492A1 (ja) * 2004-09-14 2006-03-23 Nippon Paper Industries Co.,Ltd. Ｇｌｐ−１誘導体が集積された植物及び植物貯蔵器官とその生産方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
YASUDA H. ET AL.: "Expression of the small peptide GLP-1 in transgenic plants", TRANSGENIC RES., vol. 14, no. 5, 2005, pages 677 - 684, XP019269468 *
YASUDA H. ET AL.: "The correlation between expression and localization of a foreign gene product in rice endosperm", PLANT CELL PHYSIOL., vol. 47, no. 6, 2006, pages 756 - 763, XP008112037 *

Also Published As

Publication number Publication date
JPWO2008087998A1 (ja) 2010-05-13
AU2008205931B2 (en) 2013-04-04
US20100088780A1 (en) 2010-04-08
AU2008205931A1 (en) 2008-07-24
JP5206419B2 (ja) 2013-06-12
CA2675364A1 (en) 2008-07-24
EP2119779A4 (en) 2010-09-08
EP2119779A1 (en) 2009-11-18

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