WO2008015419A3 - Protein solubilisation - Google Patents

Protein solubilisation Download PDF

Info

Publication number
WO2008015419A3
WO2008015419A3 PCT/GB2007/002914 GB2007002914W WO2008015419A3 WO 2008015419 A3 WO2008015419 A3 WO 2008015419A3 GB 2007002914 W GB2007002914 W GB 2007002914W WO 2008015419 A3 WO2008015419 A3 WO 2008015419A3
Authority
WO
WIPO (PCT)
Prior art keywords
solution
protein solubilisation
protein
solubilisation
maintaining
Prior art date
Application number
PCT/GB2007/002914
Other languages
French (fr)
Other versions
WO2008015419A2 (en
Inventor
Paul Fyfe
Original Assignee
Univ Dundee
Paul Fyfe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Dundee, Paul Fyfe filed Critical Univ Dundee
Priority to GB0902200A priority Critical patent/GB2453901B/en
Publication of WO2008015419A2 publication Critical patent/WO2008015419A2/en
Publication of WO2008015419A3 publication Critical patent/WO2008015419A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a method of identifying optimal or desirable conditions for maintaining a selected protein in solution for enabling proteins to be kept in solution at high enough concentrations for structural analysis and the like.
PCT/GB2007/002914 2006-08-02 2007-08-01 Protein solubilisation WO2008015419A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB0902200A GB2453901B (en) 2006-08-02 2007-08-01 Protein solubilisation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0615312A GB0615312D0 (en) 2006-08-02 2006-08-02 Protein Solubilisation
GB0615312.6 2006-08-02

Publications (2)

Publication Number Publication Date
WO2008015419A2 WO2008015419A2 (en) 2008-02-07
WO2008015419A3 true WO2008015419A3 (en) 2008-03-20

Family

ID=37027070

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2007/002914 WO2008015419A2 (en) 2006-08-02 2007-08-01 Protein solubilisation

Country Status (2)

Country Link
GB (2) GB0615312D0 (en)
WO (1) WO2008015419A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2476442C2 (en) 2007-03-29 2013-02-27 Эббот Лэборетриз Crystalline human il-12 antibodies
US8883146B2 (en) 2007-11-30 2014-11-11 Abbvie Inc. Protein formulations and methods of making same
US8557253B2 (en) 2009-10-07 2013-10-15 Sanofi Pasteur Sa Stabilizing excipient for inactivated whole virus vaccine
CN107290545B (en) * 2017-06-19 2019-01-18 威海威仕泰医疗科技有限公司 A kind of stable β2-microglobulin solution, preparation method and application

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017526A1 (en) * 2003-08-05 2005-02-24 The Research Foundation Of State University Of New York Reconstitution medium for protein and peptide formulations

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017526A1 (en) * 2003-08-05 2005-02-24 The Research Foundation Of State University Of New York Reconstitution medium for protein and peptide formulations

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "EZQ Protein Quantitation Kit (R33200)", MOLECULAR PROBES - INVITROGEN DETECTION TECHNOLOGIES, 8 July 2005 (2005-07-08), pages 1 - 4, XP002463960, Retrieved from the Internet <URL:http://wolfson.huji.ac.il/purification/PDF/Protein_Quantification/MOLECULAR_PROBES_EZQProtQuantKit.pdf> [retrieved on 20080110] *
BONDOS S E ET AL: "Detection and prevention of protein aggregation before, during, and after purification.", ANALYTICAL BIOCHEMISTRY, vol. 316, no. 2, 15 May 2003 (2003-05-15), pages 223 - 231, XP009094194, ISSN: 0003-2697 *
JANCARIK J ET AL: "Optimum solubility (OS) screening: an efficient method to optimize buffer conditions for homogeneity and crystallization of proteins.", ACTA CRYSTALLOGRAPHICA. SECTION D, BIOLOGICAL CRYSTALLOGRAPHY, vol. 60, no. Pt 9, September 2004 (2004-09-01), pages 1670 - 1673, XP009094193, ISSN: 0907-4449 *
KAWATE T ET AL: "Fluorescence-Detection Size-Exclusion Chromatography for Precrystallization Screening of Integral Membrane Proteins", STRUCTURE, CURRENT BIOLOGY LTD., PHILADELPHIA, PA, US, vol. 14, no. 4, April 2006 (2006-04-01), pages 673 - 681, XP005387802, ISSN: 0969-2126 *
LE MAIRE M ET AL: "Interaction of membrane proteins and lipids with solubilizing detergents", BIOCHIMICA ET BIOPHYSICA ACTA - BIOMEMBRANES, vol. 1508, no. 1-2, 23 November 2000 (2000-11-23), NETHERLANDS, pages 86 - 111, XP004273339, ISSN: 0005-2736 *
WELLING-WESTER S ET AL: "Effect of detergents on the structure of integral membrane proteins of Sendai virus studied with size-exclusion high-performance liquid chromatography and monoclonal antibodies.", JOURNAL OF CHROMATOGRAPHY, vol. 443, 29 June 1988 (1988-06-29), pages 255 - 266, XP009094232, ISSN: 0021-9673 *

Also Published As

Publication number Publication date
GB0615312D0 (en) 2006-09-13
GB2453901B (en) 2011-07-13
GB0902200D0 (en) 2009-03-25
GB2453901A (en) 2009-04-22
WO2008015419A2 (en) 2008-02-07

Similar Documents

Publication Publication Date Title
WO2008143679A9 (en) Nucleic acids and proteins and methods for making and using them
IL195183A0 (en) Method for the extraction of one or several proteins present in milk
PL1883474T3 (en) System for the integrated and automated analysis of dna or protein and method for operating said type of system
EP2001990A4 (en) Integrated system for processing microfluidic samples, and method of using same
KR101369640B1 (en) Method for identifying useful proteins of brewery yeast
FI20051248A0 (en) Biological Component Enrichment Unit and Enrichment Method
EP2059325A4 (en) Oxygen concentration system and method
EP1971437A4 (en) Method and apparatus for the filtration of biological samples
EP2021966A4 (en) Probabilstic stress wave analysis system and method
AU2013207599A1 (en) Process for determining one or more analytes in samples of biological origin having complex composition, and use thereof
EP1873723A4 (en) Method for preparing and running vote process with the aid of an automated system
GB0506880D0 (en) Method and apparatus for the detection of biological molecules
GB2439032B (en) Method and system for sample testing
AU2006341191A1 (en) System and method for automating workflow
EP2143043A4 (en) System and method for the automated analysis of cellular assays and tissues
EP2029783A4 (en) Biological fixative and method of using the biological fixative
WO2009098682A3 (en) Methods and compositions for treatment of mitochondrial disorders
EP1893978A4 (en) Method and system for affinity analysis
WO2008085962A3 (en) Cell culture methods for producing recombinant proteins in the presence of reduced levels of one or more contaminants
EP1959021A4 (en) Method for determination of cholesterol level in remnant-like lipoprotein, reagent and kit
GB2439437B (en) Method for determining the concentration of nucleic acids
EP1998166A4 (en) Evaluation method for photosynthesis sample, evaluation system for photosynthesis sample, and evaluation program for photosynthesis sample
EP1878801A4 (en) Protein cleavage method and use thereof
GB0505777D0 (en) Enzyme detection method and reagent
WO2008015419A3 (en) Protein solubilisation

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07789088

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 0902200

Country of ref document: GB

Kind code of ref document: A

Free format text: PCT FILING DATE = 20070801

WWE Wipo information: entry into national phase

Ref document number: 0902200.5

Country of ref document: GB

NENP Non-entry into the national phase

Ref country code: RU

122 Ep: pct application non-entry in european phase

Ref document number: 07789088

Country of ref document: EP

Kind code of ref document: A2