GB2453901A - Protein solubilisation - Google Patents

Protein solubilisation Download PDF

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Publication number
GB2453901A
GB2453901A GB0902200A GB0902200A GB2453901A GB 2453901 A GB2453901 A GB 2453901A GB 0902200 A GB0902200 A GB 0902200A GB 0902200 A GB0902200 A GB 0902200A GB 2453901 A GB2453901 A GB 2453901A
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GB
United Kingdom
Prior art keywords
solution
protein solubilisation
protein
solubilisation
maintaining
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB0902200A
Other versions
GB2453901B (en
GB0902200D0 (en
Inventor
Paul Fyfe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Dundee
Original Assignee
University of Dundee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Dundee filed Critical University of Dundee
Publication of GB0902200D0 publication Critical patent/GB0902200D0/en
Publication of GB2453901A publication Critical patent/GB2453901A/en
Application granted granted Critical
Publication of GB2453901B publication Critical patent/GB2453901B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a method of identifying optimal or desirable conditions for maintaining a selected protein in solution for enabling proteins to be kept in solution at high enough concentrations for structural analysis and the like.

Description

GB 2453901 A continuation (56) cont ACTA CRYSTALLOGRAPHICA. SECTION D, BIOLOGICAL CRYSTALLOGRAPHY, Vol. 60, 2004, J Janarik et al, "Optimum solubility (OS) screening: an efficient method to optimize buffer conditions for homogeneity and crystallization of proteins.", 1670-1673
BIOCHIMICA ET BIOPHYSICA ACTA -
BIOMEMBRANES, Vol. 1508, 2000, M Le Maire et al, "Interaction of membrane proteins and lipids with solubilizing detergents", 86-111
(58) Field of Search by ISA:
INT CL GO1N Other: ONLINE: EPO-Internal, WPI Data, BIOSIS,
EM BASE
GB0902200A 2006-08-02 2007-08-01 Protein solubilisation Expired - Fee Related GB2453901B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0615312A GB0615312D0 (en) 2006-08-02 2006-08-02 Protein Solubilisation
PCT/GB2007/002914 WO2008015419A2 (en) 2006-08-02 2007-08-01 Protein solubilisation

Publications (3)

Publication Number Publication Date
GB0902200D0 GB0902200D0 (en) 2009-03-25
GB2453901A true GB2453901A (en) 2009-04-22
GB2453901B GB2453901B (en) 2011-07-13

Family

ID=37027070

Family Applications (2)

Application Number Title Priority Date Filing Date
GB0615312A Ceased GB0615312D0 (en) 2006-08-02 2006-08-02 Protein Solubilisation
GB0902200A Expired - Fee Related GB2453901B (en) 2006-08-02 2007-08-01 Protein solubilisation

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB0615312A Ceased GB0615312D0 (en) 2006-08-02 2006-08-02 Protein Solubilisation

Country Status (2)

Country Link
GB (2) GB0615312D0 (en)
WO (1) WO2008015419A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2476442C2 (en) 2007-03-29 2013-02-27 Эббот Лэборетриз Crystalline human il-12 antibodies
US8883146B2 (en) 2007-11-30 2014-11-11 Abbvie Inc. Protein formulations and methods of making same
US8557253B2 (en) 2009-10-07 2013-10-15 Sanofi Pasteur Sa Stabilizing excipient for inactivated whole virus vaccine
CN107290545B (en) * 2017-06-19 2019-01-18 威海威仕泰医疗科技有限公司 A kind of stable β2-microglobulin solution, preparation method and application

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2534565C (en) * 2003-08-05 2012-06-19 The Research Foundation Of State University Of New York Reconstitution medium for protein and peptide formulations

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ACTA CRYSTALLOGRAPHICA. SECTION D, BIOLOGICAL CRYSTALLOGRAPHY, Vol. 60, 2004 , J Janarik et al, "Optimum solubility (OS) screening: an efficient method to optimize buffer conditions for homogeneity and crystallization of proteins.", 1670-1673 *
ANALYTICAL BIOCHEMISTRY, Vol. 316, 2003, S Bondos et al, "Detection and prevention of protein aggregation before, during, and after purification.", 223-231 *
BIOCHIMICA ET BIOPHYSICA ACTA - BIOMEMBRANES, Vol. 1508, 2000, M Le Maire et al, "Interaction of membrane proteins and lipids with solubilizing detergents", 86-111 *
JOURNAL OF CHROMATOGRAPHY, Vol. 443, 988, S Welling-Wester et al, "Effect of detergents on the structure of integral membrane proteins of Sendai virus studied with size-exclusion high-performance liquid chromatography and monoclonal antibodies.", 255-266 *
MOLECULAR PROBES - INVITROGEN DETECTION TECHNOLOGIES, [Online] 8 July 2005, Anonymous, "EZQ Protein Quantitation Kit (R33200)" pages 1-4. Retrieved from the Internet URL: http://wolfson.huji.ac.il/purification/PDF/Protein_Quantification/MOLECULAR_PROBES_EZQProtQuantKit.pdf *
STRUCTURE, Vol. 14, 2006, T Kawate et al, "Fluorescence-Detection Size-Exclusion Chromatography for Precrystallization Screening of Integral Membrane Proteins", 673-681 *

Also Published As

Publication number Publication date
WO2008015419A3 (en) 2008-03-20
GB0615312D0 (en) 2006-09-13
GB2453901B (en) 2011-07-13
GB0902200D0 (en) 2009-03-25
WO2008015419A2 (en) 2008-02-07

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PCNP Patent ceased through non-payment of renewal fee

Effective date: 20120801