WO2007114454A1 - Procédé de production d'un composé thioester peptidique - Google Patents
Procédé de production d'un composé thioester peptidique Download PDFInfo
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- WO2007114454A1 WO2007114454A1 PCT/JP2007/057508 JP2007057508W WO2007114454A1 WO 2007114454 A1 WO2007114454 A1 WO 2007114454A1 JP 2007057508 W JP2007057508 W JP 2007057508W WO 2007114454 A1 WO2007114454 A1 WO 2007114454A1
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
- C07K1/042—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers characterised by the nature of the carrier
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K9/00—Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof
Definitions
- the present invention relates to a method for producing a peptide thioester compound.
- glycoproteins having sugar chains Many of proteins present in the living body are glycoproteins having sugar chains.
- Sugar chains in glycoproteins play a role in maintaining the three-dimensional structure of proteins, regulating solubility, and adding protease resistance.
- sugar chains in glycoproteins are involved in life phenomena such as fertilization, differentiation, signal transduction, canceration, intracellular transport of proteins and regulation of physiological activities.
- sugar chains bound to proteins play an important role in various physiological functions.
- the structures of these sugar chains are diverse and the number of types is enormous, it is extremely difficult to identify which structure of sugar chains is involved in life phenomena. In order to understand these functions, it is essential to synthesize glycoproteins and glycopeptides with a single-structure sugar chain.
- glycoproteins At present, it is possible to express glycoproteins using protein expression in biological methods, but it is difficult to obtain glycoproteins with uniform sugar chains. Therefore, in recent years, the chemical synthesis of glycopeptides and glycoproteins having a sugar chain with a single structure has been studied.
- the present inventors have prepared a method of preparing a large amount of biantennary complex-type sugar chains that can be used as raw materials from chicken eggs by combining enzymatic methods and chemical methods (Patent Document 1), and the complex-type sugar chains can be used as peptide solid phases.
- Patent Document 1 a method for synthesizing sialyl glycopeptides (Patent Document 2). If glycopeptides can be polymerized, it will be possible to synthesize large glycoproteins with a single-structure sugar chain.
- the N ati V ec hemi calligation method is used to couple peptides with peptide fragments of cysteine (Cys) and thioesters at the C terminus.
- Non-patent document 1) is considered to be the most effective.
- a solid phase synthesis method is generally used in which an amino acid protected at the N-terminus is immobilized on an insoluble resin carrier, the protecting group of amino acid is deprotected, and then the peptide chain is extended sequentially. Used.
- a method for producing a peptide having a thioester at the C-terminus a method of thioesterification is performed when the peptide is cleaved from the solid phase. Methods and the like.
- Non-patent Document 1 a method for thioesterification when a peptide is cleaved from a solid phase
- a method for thioesterification when a peptide is cleaved from a solid phase for example, a method is known in which a peptide is produced using a Safety Cat Linker on a solid phase resin and a thiol compound is allowed to act (Non-patent Document 1). 2).
- problems such as poor condensation efficiency when immobilizing the first amino acid on the resin, slight racemization of the amino acid during the condensation, and poor esterification reactivity with thiol compounds. have.
- the alkylation performed for the activation of the Safety Catch Linkage will easily alkylate the sugar hydroxyl group.
- a strong acid such as 95% trifluoroacetic acid hydrogen fluoride is usually used.
- the use of such strong acids is accompanied by deprotection of peptide side chains and cleavage of glycopeptides.
- Non-Patent Documents 3, 4, 5 a method using a trityl resin as a solid phase and using acetic acid for the cleaving
- a method using a trityl resin as a solid phase and using acetic acid for the cleaving (Non-Patent Documents 3, 4, 5) or 4-hydroxymethy
- TFA trifluoroacetic acid
- HMPB resin rho-3-methoxyphenoxybutyric acid
- Non-patent document 6 Non-patent document 6
- the method using a trityl resin cannot produce a glycopeptide that does not protect the hydroxyl group.
- a glycopeptide is prepared using HMPB resin as a solid phase, it cannot be cleaved with 1% TFA, and when 10% TFA is used, a part of the protecting group of the peptide side chain is removed. Protected.
- thioesterifying a peptide protection of the thiol group of the N-terminal cysteine is essential to prevent self-condensation, but deprotection at the time of excision will cause a fatal result in the next reaction. . Therefore, these methods are not sufficient as a method for producing a peptide having a force lupoxyl group as a raw material for producing a peptide having a thioester at the C-terminus.
- Peptide thioesters can be prepared by reacting side-chain-protected peptides with alkylthiols, but there is a problem that the C-terminal amino acid racemizes. In order to avoid racemization, the amino acid at the C-terminal is replaced with glycine (Non-patent Document 7).
- DCM dichloromethane
- PyBOP benzotriazole 1-yloxy sheet pyrrolidinophosphonium hexafluorophosphate
- Non-patent Document 8 Method using Z-diisopropylethylamine (DI PEA) (Non-patent document 8) In tetrahydrofuran (THF), 2 _ (1H_benzotriazole-1) as a condensing agent —1, 1 , 3, 3-Tetramethyluronium hexafluorophosphite (HBTU) ZD I PEA (Non-patent Document 9) has been reported.
- the method of using glycine as the C-terminal amino acid naturally limits the peptides to be produced.
- the glycopeptide having a hydroxyl group that is not protected by a protecting group cannot be dissolved in a solvent such as DCM or THF, and the solvent must be changed.
- the racemization problem of the C-terminal amino acid is repeated. It will occur.
- Patent Document 1 WO 03/008431
- Patent Document 2 WO 2004/005330
- Non-Patent Document 1 J. Am. Chem. Soc., 121, 11369-11374 (1999)
- Non-Patent Document 2 An g ew. Ch em. Int. Ed., 44, 165 0-1654 (2005)
- Non-Patent Document 5 J. Am. Chem. Soc., 123, 3885-3 891 (2001)
- Non-Patent Document 9 J. Am. Chem. Soc., 123, 3885-3 891 (2001)
- the object of the present invention is to maintain peptide side chain protecting groups, which are applicable not only to peptides having no sugar chain, but also to sugar chains, particularly glycopeptides having a sugar chain whose hydroxyl group is not protected.
- Another object of the present invention is to provide a method for producing a peptide having a carboxyl group at the C-terminus.
- the object of the present invention is to suppress the formation of a racemate, which is applicable not only to peptides having no sugar chain, but also to sugar chains, particularly glycopeptides having a sugar chain whose hydroxyl group is not protected.
- An object of the present invention is to provide an efficient method for producing a peptide thioester compound. Disclosure of the invention
- the present invention relates to the following inventions.
- a resin modified with a linker represented by the formula (1) is used as a solid phase, and a peptide is formed by a solid phase synthesis method.
- (C) A method for producing a thioester compound of a peptide, comprising reacting a thiol compound in a solvent in the presence of a condensing agent at 100 to 0 ° C.
- R 1 represents an alkyl group
- R 2 represents a hydrogen atom
- n represents an integer of 1 to 4.
- the present inventors use a specific solid phase resin and excise the peptide produced under specific conditions from the solid phase resin, so that the sugar chain structure is not affected, and the C-terminus maintaining the side chain protecting group is used. It has been found that peptides having a lupoxyl group can be produced.
- the method for producing a thioester compound of a peptide of the present invention comprises (A) using a resin modified with a linker represented by the formula (1) as a solid phase, forming a peptide by a solid phase synthesis method, (B) Cleave the bond between the solid phase and the peptide with at least one acid selected from dilute hydrochloric acid, dilute sulfuric acid, formic acid, and acetic acid to produce a peptide having a strong loxyl group at the C-terminus. It comprises a step of reacting the thiol compound in the presence of a condensing agent at 1 100 to 0 ⁇ .
- R 1 represents a 4 alkyl group
- R 2 represents a hydrogen atom or an alkoxy group
- n represents an integer of 1 to 4.
- Alkyl groups include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, etc. 1 to 4 alkyl groups are shown.
- Ci- 4 alkoxy groups include linear or branched methoxy groups, ethoxy groups, n-propoxy groups, isopropoxy groups, n-butoxy groups, isobutoxy groups, sec-butoxy groups, tert-butoxy groups, etc. An alkoxy group having 1 to 4 carbon atoms is shown.
- the resin modified with the linker represented by the formula (1) may be a commercially available resin or a conventionally known resin, such as HMPB-BHA resin (4-hydroxymethyl-3-methoxyphenoxybutyric acid). Benzhydrylamine), HMPB—MBH A resin (4-hydroxymethyl-3-methoxyphenoxybutyric acid monomethylbenzhydrylamine), etc., but in the production of glycopeptides, Ami no—PEGA resin (Novabiochem) Swellability obtained by reacting a carboxylic group of a powerful sulfonic acid compound represented by the formula (2) in the presence of a dehydrating condensing agent according to a conventionally known amidation reaction. An excellent resin can be used.
- PEGA resin is bisacrylamide probe 1-ethylene polyethylene glycol.
- a resin in which R 1 is a methyl group, R 2 is a hydrogen atom and an alkyl group is preferable, and R 1 is a methyl group and R 2 is a hydrogen atom, - 4 alkyl group, preferably a resin which n is 2 to 4, R 1 turtles methyl group, R 2 is a hydrogen atom, - 4 alkyl group, resin n is 3 is particularly preferable.
- a resin represented by the formula (3) is particularly preferable.
- a peptide is produced using a resin modified with a linker represented by the above formula (1) as a solid phase.
- Any amino acid can be used, such as serine (Ser), asparagine (As n), parin (Val), leucine (Leu), isoleucine (I 1 e), alanine (A 1 a) , Tyrosine (Ty r), glycine (G 1 y), lysine (L ys), arginine (A rg), histidine (His), aspartate (As p), glutamate (G 1 u), glutamine (G 1 n), threonine (Thr), cysteine (Cy s), methionine (Me t), phenylalanine (Phe), triftophan (Tr p), proline (Pro).
- Protecting groups include, for example, carbonate-containing groups such as 9-fluoroenylmethoxycarbonyl (Fmo c) group, t-butyloxycarbonyl (Bo c) group, aralkyloxycarbonate (A 1 1 oc) group, Protecting groups such as an acyl group such as an acetyl group (Ac), an aryl group and a benzyl group can be exemplified.
- a protecting group for example, when introducing an Fmoc group, it can be introduced by adding 9-fluorenylmethyl-N-succinimidyl carbonate and sodium hydrogen carbonate. The reaction is carried out at 0 to 50 ° C., preferably at room temperature, for about 1 to 5 hours.
- An amino acid whose amino group is protected with a fat-soluble protecting group can be produced by introducing a fat-soluble protecting group into the amino group of the above-mentioned amino acid by the above-mentioned method or a known method, and a commercially available one is also used. can do.
- amino acids in which the amino group is protected with the Fmo c group include Fmo c 1 Ser, Fmo c 1 A sn, Fmo c -V a 1, Fmo c — L eu, Fmo c 1 I 1 e, Fmo c 1 AI a, Fmo c-1 T yr, Fmo c-G 1 y, Fmo c-Lys, Fmo c-1 A rg, Fmo c-1 H is, Fmo c-As p, Fmo c-G 1 u, Fmo c-1 G 1 n, Fmo c-1 T hr, Fmo c-Cys, Fmo c -Me t, Fmo c -Phe, Fmo c-1 T rp, Fmo c-Pro.
- cysteine (Cys) is selected as the last amino acid to be introduced, a peptide having a cysteine at the N-terminus can be produced. It can be used as a fragment to be force-coupled with thiol ester compound of peptide in ca 1 Ligation.
- sugar chain amino acid in which a sugar chain is bound to an amino acid
- a sugar peptide in which the sugar chain amino acid is introduced at an arbitrary position of the peptide chain can be produced.
- the sugar chain amino acid to be used is not particularly limited as long as it has an arbitrary number of sugar residues, and is a high mannose rich in mannose, a complex type having a sialic acid or galactose residue at the non-reducing end of the sugar chain.
- Sugar chain ( Figure 1), mixed sugar chain with high mannose structure and complex sugar chain, amide group of side chain of asparagine and N-glycosylated N-linked sugar chain, side chain of serine, threonine
- Examples include ⁇ -linked sugar chains in which the alcohols are glycosylated.
- Specific examples include sugar chain asparagine described in WO 0 3/0 0 8 4 3 1. Among them, disialic sugar chain asparagine or monosialo sugar chain asparagine is preferable.
- a sugar chain in which the force loxyl group of the sialic acid represented by (4) is protected by a protecting group such as a benzyl group.
- a glycosyl sugar chain asparagine or a glycopeptide to which a monosaccharide mouth sugar chain asparagine is bound is a preferred glycopeptide.
- the esterification reaction between the hydroxyl group of a part of the linker of the resin and the ability of the amino acid having the protected amino group, lpoxyl group is, for example, 1-mesitylenesulfonyl-3-2- Can be performed using a dehydrating condensing agent such as Tallow 1, 2, 4 monobasic riazole (MSNT), DCC, or diisopropyl carpositimide (DI PCD I).
- a resin is placed in a solid phase ram and washed with a solvent. Then, it is preferably carried out by adding a solvent solution of the amino acid.
- washing solvent examples include DMF, 2-propanol, DCM, and the like.
- solvents that dissolve amino acids include DMSO, DMF, and DCM.
- the reaction is carried out at 0 to 50 ° C., preferably at room temperature, for about 10 minutes to 30 hours, preferably about 15 minutes to 24 hours.
- the amino-protecting group can be deprotected by treatment with an acid or a base.
- a base such as piperidine or morpholine can be used.
- it is preferably carried out in the presence of a solvent, and examples thereof include DMS 0, DMF, and methanol.
- reaction of amidating an unprotected amino group with the force loxyl group of any amino acid in which the amino group is protected is preferably carried out in the presence of an activator and a solvent.
- Activating agents include, for example, dicyclohexyl carpositimide (DCC), 1-ethyl-3- (3-dimethylaminopropyl) carpositimide, hydrochloride (WS C / HC 1), diphenyl phosphoryl azide (DPPA) , Force sulfonyl diimidazole (CD I), jetyl cyanophosphonate (DEPC), diisopropyl carpoimide (DI PC I), benzotriazole— 1-yloxy sheet pyrrolidinophosphonium hexafluorophosphate (PyBOP), 1 —Hydroxybenzotriazol (HOB t), hydroxysuccinimide (HOS u), dimethylaminopyridine (DMAP), 1-hydroxy-7-azabenzotriazol (HOA t), hydroxyfurimide (HOP ht) , Pentafluorophenol (P fp—OH), 2— (1H—benzotri
- the solvent examples include dimethyl sulfoxide (DMSO), N, N-dimethylformamide (DMF), dichloromethane (DCM) and the like.
- the amount of the activator used is 0.1 to 20 equivalents, preferably 0.5 to 10 equivalents, more preferably 0.8 to 5 equivalents relative to the carboxylic acid compound represented by the formula (2). Is preferred.
- the reaction can be carried out in a solvent, and it may be carried out at 0 to 50, preferably at room temperature for about 10 to 30 hours, preferably about 15 minutes to 24 hours. In addition, it is preferable to perform the reaction in a column for solid phase synthesis, which can be used as it is for the subsequent solid phase synthesis.
- the removal of the protecting group can be carried out in the same manner as described above.
- an acid In order to cleave the peptide chain from the resin (resin), it is preferable to treat with an acid.
- the acid used include mineral acids such as dilute hydrochloric acid and dilute sulfuric acid, and carboxylic acids such as formic acid and acetic acid.
- dilute hydrochloric acid or dilute sulfuric acid include aqueous solutions of hydrochloric acid or sulfuric acid of about 0.01 to 2 N, preferably about 0.05 to 1 N. Of these acids, acetic acid is preferred.
- the amount of the acid used is not particularly limited as long as it is 1 equivalent or more with respect to 1 equivalent of peptide. For example, it may be about 1 to 10,000 equivalents, preferably about 10 to 1000 equivalents.
- This reaction is preferably carried out in the presence of alcohol, and examples thereof include lower alcohols such as methanol, ethanol and propanol, and halogenoalcohols such as trifluoroethanol (TFE) and trichloroethanol.
- lower alcohols such as methanol, ethanol and propanol
- halogenoalcohols such as trifluoroethanol (TFE) and trichloroethanol.
- TFE trifluoroethanol
- methanol, trifluoroethanol power S is preferable
- trifluoroethane Nord is particularly preferred.
- the alcohol may be used in an amount of 0.1 to 2 volumes, preferably 0.5 to 1.5 volumes, more preferably 0.8 to 1.2 volumes of alcohol with respect to 1 volume of acid.
- this reaction may use an organic solvent such as DCM, DMF, or DMSO.
- the amount of the solvent used is not particularly limited, and may be about 0.1 to 100 volumes with respect to 1 volume of acid.
- the reaction is carried out at 0 to 50 ° C., preferably at room temperature, for about 1 to 30 hours.
- a peptide having a strong lpoxyl group at the C-terminal can be obtained.
- a peptide whose C-terminal is a thioester can be produced.
- a thiol compound an arbitrary number of halogen atoms such as fluorine, chlorine, bromine and iodine, a lower alkyl group having 1 to 4 carbon atoms such as a methyl group and an ethyl group, and a methoxy group at any position on the phenyl ring ,
- An alkoxy group having 1 to 4 carbon atoms such as an ethoxy group, a benzyl mercaptan which may have a substituent such as a nitro group, a lower alkyl thiol such as methanethiol and ethanethiol, etc. be able to.
- the benzylmer force compound is particularly preferred.
- the amount of the thiol compound used may be 1 to 100 equivalents, preferably 10 to 80 equivalents, more preferably 20 to 50 equivalents per equivalent of the starting peptide.
- Examples of the solvent to be used include THF, DCM, DMSO, DMF, etc. Among them, DMF is preferable.
- Examples of the condensing agent include HOB t / DI PC I or PyBOP / DI PEA, and PyBOPZD I PEA is preferable.
- the ratio of HOB t / DI PC I used is 0.1 to 10 equivalents, preferably 0.5 to 5 equivalents, more preferably 0.8 to 1.2 equivalents of DI PC I with respect to 1 equivalent of HOB t. .
- the proportion of PyBOP / DI PEA used is 0.1 to 10 equivalents, preferably 0.5 to 5 equivalents, more preferably 0.8 to 1.2 equivalents of DI PEA to 1 equivalent of Py BOP. .
- the amount of HOB t used may be 1 to 20 equivalents, preferably 3 to 15 equivalents, more preferably 8 to 12 equivalents per equivalent of the starting peptide.
- the use ratio of PyBOP may be 1 to 10 equivalents, preferably 2 to 8 equivalents, more preferably 3 to 6 equivalents per equivalent of the starting peptide.
- the reaction is preferably performed by mixing the raw peptide and the thiol compound and then adding a condensing agent.
- the reaction temperature may be ⁇ 100 to 0 ° C., preferably ⁇ 80 to 10 ° C., and may be performed for about 30 minutes to 2 hours.
- the ratio of the racemate of the obtained peptide is suppressed, and the ratio of the racemate is usually 6% or less, preferably 4% or less, more preferably 2% or less, and particularly preferably 0 to 1%.
- the Fmo-cylated amino acid to be used is a known amino acid and can be easily prepared by introducing a Fmoc group into a commercially available product or amino acid.
- Fmo c- A la, Fmo c- As n, Fmo c- G ly, Fmo c- Leu, Fmo c-Me t, Fmo c- Phe, Fmo c- Pro, Fmo c- Va 1 are amino acids. Means that the amino group is protected with an Fmo c group. Boc—Cys (A cm) means that the cysteine amino group is protected with a Boc group and the thiol is protected with a acetamidomethyl group. Fmo c -Ar g
- (Pb f) means NG — (2, 2, 4, 6, 7-pentamethyldihydrobenzofuran-5-sulfonyl) arginine in which the position amino group of arginine is protected with an Fmo c group.
- Fmo c—As p (O t Bu) and Fmo c—G lu (O t B u) are protected by the Fmo c group in the amino acid group of each amino acid, aspartic acid; Is protected with a tert-butyl group.
- Fmo c—Cy s (trt) means that the cysteine amino group is protected with an Fmo c group and the thiol is protected with a trityl group.
- Fmo c—Lys (Bo c) means that the ⁇ ; -position amino group of lysine is protected with an Fmo c group, and the ⁇ -position amino group is protected with a B oc group.
- Fmoc—Ser (t Bu), Fmoc-Tyr (tBu), and Fmoc—Thr (tBu) are the amino acid amino groups are Fmoc groups, and the hydroxyl groups are trityl groups. It means that it is protected by.
- Bo c_Leu and (Bo c) Leu mean that the amino group of leucine is protected by the B oc group.
- Ly s (Bo c) Ly s (Bo c) means that the lysine and ⁇ -position amino groups are protected by the Boc group, and Ly s (Bo c) means that the ⁇ -position amino group of lysine is B oc Means protected by a group.
- Bo c—Cys (Th z) means N—t—Boc— 1,3-thiazolidine_4—forced ruponic acid.
- Th r (t Bu), Ty r (t Bu) and Ser (t Bu) mean that the hydroxyl group of each amino acid is protected with a tert-butyl group.
- H is
- (trt) means that the imidazole nitrogen is protected
- Cys (trt) means that the cysteine thiol group is protected
- G ln (trt) means that the amide nitrogen of glutamine is protected with a trityl group.
- p (0 t Bu) and G 1 u ( ⁇ t Bu) mean aspartic acid; the strong lpoxyl group at the S and glutamic acid positions is protected with a tert-butyl group.
- Cys (Thz) means 1,3-thiazolidine-4-sulphonyl.
- Ar g (Pb f) means NG — (2, 24 4, 6, 7-pentamethyldihydrobenzofuran-1-5-sulfonyl) arginine.
- These protecting groups can be introduced by a conventionally known method or a commercially available product into which a protecting group has been introduced. Ph represents a phenyl group, and Bn represents a benzyl group. 11) NMR was measured with a Bruker AVANCE 400 (noted as 400 MHz).
- the ESI ma ss measuring instrument is an E squire 3000 1 13 manufactured by Bruker Dalton on ics, and the eight-mass meter is an Au tof 1 ex manufactured by Bruker D a 1 tonics. di hy drox yb e nz oicacid was used.
- Fmo c—Phe (96.8 mg, 0.25 mm o 1), MSNT (74 mg, 0.25 mm o 1) and N—methylimidazole (15 i 1, 0.19 mm o 1) were dissolved in DCM (lml). And placed in a solid phase synthesis column and stirred at room temperature for 2 hours. After stirring, the resin was washed with DCM and DMF. The Fmo c group was deprotected using 20% 20% piperidine ZDMF solution (lml) for 20 minutes. After washing with DMF, the subsequent peptide chain elongation was performed by sequentially condensing amino acids using the method shown below.
- Fmo c— Tyr (t Bu) (1 14. 9 mg, 0.25 mm o 1), Fmo c— As n (88. 6 mg, 0.25 5 mm ol), Fmo c— A 1 (77. 8 mg, 0.25 mm o 1), Fmo c— H is (trt) (154.9 mg, 0.25 mm o l), Fmo c— S er (t B u ) (95.
- the dibenzyl form (10 mg, 3.6 mo 1) and DEPBT (2 mg, 6 o 1) of sugar chain asparagine represented by the following formula (5) were dissolved in DMF (0.12 ml) and placed in an Eppendorf tube. .
- Example 1 The resin formed in Example 1 and having a 20-residue peptide was partially mixed with a power ram for solid-phase synthesis.
- the resin was removed by filtration, and the reaction solution was concentrated under reduced pressure.
- the obtained peptide had a force loxyl group at the C-terminus and maintained the side chain protecting group.
- HMPB-PEGAresin 25 ⁇ mol was obtained and used as a solid phase for solid phase synthesis to condense amino acids to form a peptide.
- the amino acid condensation was carried out in the same manner as in Example 1.
- Fmo c—A 1 a 38.9 mg, 0.13 mmo 1
- MSNT 37 mg, 0.13 mmol
- N—methy l imi dazole 7.5 jU.1 , 94 ULO 1
- DCM 0.5 ml).
- the peptide represented by formula (9) whose C-terminus is a benzylthioester (NH 2 — Cys (A cm) -As n (disia 1 oo 1 igo) -Th r -Ser -Val- I 1 e -Th r -G 1 nA 1 a -COSB n) was produced.
- Peptide Ac NH—Hi s -A 1 -A 1 a 1 P he—COOH having a strong lpoxyl group at the C-terminus was produced by a conventionally known method and used as a raw material.
- the peptide 0.5 mg, 1 ⁇ , ⁇ 1), MS 4 A (1 Omg) and benzyl mercaptan (3.7 mg, 30 mo 1) in DMF solvent (0.14 ml) under argon flow. After stirring at 20 ° C for 1 hour, PyBOP (2.6 mg, 5 ⁇ mo 1) and DIP EA (0.885 1, 5 nmo 1) were added and stirred for 17 hours.
- racemate was less than 2%.
- the benzylthioester AcNH—His-A1a-A1a—Phe_COSBn was obtained in the same manner as in Example 6 except that the reaction temperature was 0 ° C.
- the mass spectrum of the obtained compound was the same as that of Example 6.
- the production of racemate was 6%.
- Peptide AcNH— H is -A 1 a -A 1 a -P he -COOH (0.5 mg, 1 o 1) and MS 4A (1 Omg), HOB t (0.7 mg, 5 mol or 1.4 mg , 10 ⁇ mo 1) in DMF solvent (0.14 ml) under argon stream After stirring at 0 ° C for 1 hour, DI PC I (0.81, 5 / mo 1 or 1. 61, 10 umo 1) and benzyl mercaptan (3.7 mg, 30 umo 1) were added. Thereafter, the same treatment as in Example 6 was carried out to obtain a benzylthioester Ac NH—His—A 1 a—A 1 a—Phe—COSBn. The yield when using 5 equivalents of HOBt was 75%, and the yield with 10 equivalents was 98%. The mass spectrum of the obtained compound was the same as that of Example 6. Racemic formation was 5% each.
- Peptide AcNH-Cys-Cys-G1u-His-COOH having a force lpoxyl group at the C-terminus was produced by a conventionally known method and used as a raw material.
- the peptide (0.5 mg, 1 mo 1), MS 4A (1 Omg) and HOB t (1.4 mg, 10 ⁇ 1) were added in DMF solvent (0.14 ml) under an argon stream—1 at 20 ° C.
- DI PC I 1.6 il l, 10 x mol
- benzyl mercabine 3.7 mg, 30 mol 1
- racemate was less than 2%.
- a peptide was formed by sequentially condensing amino acids using Trity Chlori de ri e sin (150 mo 1) as a solid phase for solid phase synthesis. Amino acid condensation was carried out in the same manner as in Example 1.
- Fmo c—L eu (159. Omg, 0.45 mmo 1) was used as the first amino acid, and condensed with DCM (0.9 ml) and D I EA (204. 1 ⁇ 1, 1.2 mmo 1).
- Fmo c -Ty r (t B u) (334.7 mg, 0.75 mm o 1)> Fmo c -G 1 u (O t B u) (319.2 mg, 0.75 mm o 1), Fmo c — S er (t B u) (287.6mg, 0.75mmo l), Fmo c -C ys (trt) (439.3mg, 0.775mm o 1), Fmo c— As p (O t B u) (30 8. 6 mg, 0.75 mm o 1), Fmo c -V a 1 (254.
- Racemic formation rate 2% or less
- Racemic production rate 1% or less
- Racemic production rate 1% or less
- -H is -V a 1 -A 1
- Racemic formation rate 1% or less
- HMPB-PEGAresin 50 / xmol was obtained and used as a solid phase for solid phase synthesis to condense amino acids to form a peptide.
- the amino acid condensation was carried out in the same manner as in Example 1.
- Fmo c _P he (96. 9 mg, 0.25 mm o 1) was used as the first amino acid, MSNT (74.0 mg, 0.25 mm o 1), N-me thy 1 imidazole (1.9 ⁇ 1 , 187.5 mo I), and DCM (1 ml).
- Fmo c— As n (88. 6 mg, 0.25 mmol), Fmo c— Cy s (trt) (146.4 mg, 0.25 mmol), Fmo c— Lys (Bo c) (1 17. lmg, 0.25 mm o 1), Fmo c— A sn (88. 6 mg, 0.25 mm o 1), Fmo c— G ly (74. 3 mg, 0.25 mm ol), Fmo c— Ty r (t B u) (1 14.
- DI PEA (1.02 / X 1, 6 ⁇ mo 1) was added and stirred at room temperature for 20 hours. After washing with DMF, the Fmo c group is deprotected using 20% piperidine, and Phe— As n— Cy s (trt) -Ly s (Bo c) -As nG 1 A 10-residue peptide of y-Tyr (tBu) -Thr (tBu) -Lys (Boc) -Asn (O1igosaccharidechain) was formed. This 10-residue peptide was further condensed with amino acid using HOBt, DI PC I, and DMF as described above.
- Fmo c— As p (1.7 mg, 0.015 mm o 1)
- Fm o c— S e r (t B u) (1.9 mg, 0.015 mm o l)
- Fmo c -G 1 y As amino acids, Fmo c— As p (1.7 mg, 0.015 mm o 1), Fm o c— S e r (t B u) (1.9 mg, 0.015 mm o l), Fmo c -G 1 y
- racemate was less than 2%.
- a peptide having no sugar chain is affected by the sugar chain structure. It is possible to produce a peptide having a carboxyl group at the C-terminus, while maintaining the protecting group of the peptide side chain without giving any reverberation, and there is almost no racemization of the peptide C-terminus. Thioester compounds can be produced.
- the resulting peptide thioester compound can be polymerized by reacting with a peptide fragment whose N-terminal amino acid is Cy s, which enables the production of large glycoproteins with a single-structure sugar chain. It becomes.
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Abstract
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
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AU2007232742A AU2007232742B2 (en) | 2006-03-29 | 2007-03-28 | Method for production of peptide thioester compound |
CN2007800158333A CN101437836B (zh) | 2006-03-29 | 2007-03-28 | 肽的硫酯化合物的制造方法 |
US12/295,113 US8058394B2 (en) | 2006-03-29 | 2007-03-28 | Method for production of peptide thioester compound |
EP07740944.9A EP2000474B1 (fr) | 2006-03-29 | 2007-03-28 | Procédé de production d'un composé thioester peptidique |
KR1020087025264A KR101462454B1 (ko) | 2006-03-29 | 2007-03-28 | 펩티드의 티오에스테르 화합물의 제조 방법 |
JP2008508707A JP5328345B2 (ja) | 2006-03-29 | 2007-03-28 | ペプチドのチオエステル化合物の製造方法 |
CA2647867A CA2647867C (fr) | 2006-03-29 | 2007-03-28 | Procede de production d'un compose thioester peptidique |
DK07740944.9T DK2000474T3 (en) | 2006-03-29 | 2007-03-28 | PROCEDURE FOR PRODUCING A PEPTID THIOESTER COMPOUND |
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JP2006092569 | 2006-03-29 | ||
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US (1) | US8058394B2 (fr) |
EP (1) | EP2000474B1 (fr) |
JP (1) | JP5328345B2 (fr) |
KR (1) | KR101462454B1 (fr) |
CN (1) | CN101437836B (fr) |
AU (1) | AU2007232742B2 (fr) |
CA (1) | CA2647867C (fr) |
DK (1) | DK2000474T3 (fr) |
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TW (2) | TW200806686A (fr) |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010150730A1 (fr) | 2009-06-26 | 2010-12-29 | 大塚化学株式会社 | Procédé de production de thioester peptidique |
WO2013047372A1 (fr) | 2011-09-26 | 2013-04-04 | 株式会社糖鎖工学研究所 | Procédé d'obtention de fragment polypeptidique de rendement élevé, qui est approprié pour un procédé ncl |
WO2014084110A1 (fr) * | 2012-11-30 | 2014-06-05 | 株式会社糖鎖工学研究所 | Lieur à chaîne glucidique liée, composé contenant un lieur à chaîne glucidique liée et substance physiologiquement active ou son sel, et son procédé de production |
Families Citing this family (5)
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ES2624961T3 (es) | 2013-03-21 | 2017-07-18 | Sanofi-Aventis Deutschland Gmbh | Síntesis de productos de péptido que contienen imida cíclica |
AU2014234400B2 (en) | 2013-03-21 | 2017-11-16 | Sanofi-Aventis Deutschland Gmbh | Synthesis of hydantoin containing peptide products |
CN106928313B (zh) * | 2015-12-31 | 2020-12-11 | 深圳翰宇药业股份有限公司 | 一种c-端修饰肽的合成方法 |
WO2017136690A1 (fr) | 2016-02-04 | 2017-08-10 | University Of Washington | Supports solides destinés à être utilisés dans la synthèse de peptides en phase solide, trousses et procédés associés |
SG10202108699UA (en) * | 2017-03-02 | 2021-09-29 | Glytech Inc | Production method for amino-acid polymer |
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WO2003008431A1 (fr) | 2001-06-19 | 2003-01-30 | Otsuka Chemical Co.,Ltd. | Methode de production d'un derive d'asparagine de chaines des sucres |
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CA2265900C (fr) * | 1996-09-09 | 2007-07-31 | Zealand Pharmaceuticals A/S | Amelioration apportee a une synthese de peptides en phase solide et agent utilise dans ladite synthese |
DE10216513A1 (de) * | 2002-04-10 | 2003-10-23 | Joerg Rademann | Polymerverbindung aufgebaut aus linearen Polymerketten |
WO2005095331A1 (fr) | 2004-03-31 | 2005-10-13 | Shionogi & Co., Ltd. | Agent de liaison d’une chaîne de sucre à un peptide |
CN1254484C (zh) * | 2004-04-30 | 2006-05-03 | 吉林省一心制药有限公司 | 醋酸奥曲肽的固相合成方法 |
EP1961764B1 (fr) | 2005-11-30 | 2011-05-11 | Shionogi Co., Ltd. | Produit d'addition de chaine de sucres et de peptide et produit pharmaceutique comprenant ce produit en tant que principe actif |
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- 2007-03-28 WO PCT/JP2007/057508 patent/WO2007114454A1/fr active Application Filing
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WO2004005330A1 (fr) | 2002-07-05 | 2004-01-15 | Otsuka Chemical Co., Ltd. | Procede de production d'un peptide de sucre comprenant une chaine de sucre d'asparagine et peptide de sucre resultant |
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Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2010150730A1 (fr) | 2009-06-26 | 2010-12-29 | 大塚化学株式会社 | Procédé de production de thioester peptidique |
KR20120039637A (ko) | 2009-06-26 | 2012-04-25 | 오츠카 가가쿠 가부시키가이샤 | 펩티드 티오에스테르체의 제조방법 |
CN102803286A (zh) * | 2009-06-26 | 2012-11-28 | 大塚化学株式会社 | 肽硫酯的制造方法 |
US8642725B2 (en) | 2009-06-26 | 2014-02-04 | Glytech Inc. | Process for production of peptide thioester |
RU2529998C2 (ru) * | 2009-06-26 | 2014-10-10 | Глитек,Инк. | Способ получения тиоэфира пептида |
JP5730197B2 (ja) * | 2009-06-26 | 2015-06-03 | 株式会社糖鎖工学研究所 | ペプチドチオエステル体の製造方法 |
US9127041B2 (en) | 2009-06-26 | 2015-09-08 | Glytech, Inc. | Process for production of peptide thioester |
WO2013047372A1 (fr) | 2011-09-26 | 2013-04-04 | 株式会社糖鎖工学研究所 | Procédé d'obtention de fragment polypeptidique de rendement élevé, qui est approprié pour un procédé ncl |
WO2014084110A1 (fr) * | 2012-11-30 | 2014-06-05 | 株式会社糖鎖工学研究所 | Lieur à chaîne glucidique liée, composé contenant un lieur à chaîne glucidique liée et substance physiologiquement active ou son sel, et son procédé de production |
US10202469B2 (en) | 2012-11-30 | 2019-02-12 | Glytech, Inc. | Sugar chain-attached linker, compound containing sugar chain-attached linker and physiologically active substance or salt thereof, and method for producing same |
Also Published As
Publication number | Publication date |
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SG170022A1 (en) | 2011-04-29 |
JPWO2007114454A1 (ja) | 2009-08-20 |
AU2007232742A1 (en) | 2007-10-11 |
CA2647867C (fr) | 2015-11-03 |
TWI353989B (fr) | 2011-12-11 |
US8058394B2 (en) | 2011-11-15 |
KR20090007709A (ko) | 2009-01-20 |
EP2000474A4 (fr) | 2012-05-09 |
TWI450905B (zh) | 2014-09-01 |
DK2000474T3 (en) | 2018-01-22 |
CN101437836A (zh) | 2009-05-20 |
CA2647867A1 (fr) | 2007-10-11 |
EP2000474B1 (fr) | 2017-11-01 |
TW201109347A (en) | 2011-03-16 |
CN101437836B (zh) | 2013-07-24 |
KR101462454B1 (ko) | 2014-11-17 |
US20090137780A1 (en) | 2009-05-28 |
AU2007232742B2 (en) | 2012-02-02 |
EP2000474A1 (fr) | 2008-12-10 |
JP5328345B2 (ja) | 2013-10-30 |
TW200806686A (en) | 2008-02-01 |
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