WO2007102439A1 - Fractionation product of aqueous extract of eucommia ulmoides oliver leaf, and composition for oral ingestion comprising the fractionation product - Google Patents

Fractionation product of aqueous extract of eucommia ulmoides oliver leaf, and composition for oral ingestion comprising the fractionation product Download PDF

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Publication number
WO2007102439A1
WO2007102439A1 PCT/JP2007/054086 JP2007054086W WO2007102439A1 WO 2007102439 A1 WO2007102439 A1 WO 2007102439A1 JP 2007054086 W JP2007054086 W JP 2007054086W WO 2007102439 A1 WO2007102439 A1 WO 2007102439A1
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WIPO (PCT)
Prior art keywords
leaf
fraction
extract
food
nakanaka
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PCT/JP2007/054086
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French (fr)
Japanese (ja)
Inventor
Tetsuya Hirata
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Kobayashi Pharmaceutical Co., Ltd.
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Publication of WO2007102439A1 publication Critical patent/WO2007102439A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/46—Eucommiaceae (Eucommia family), e.g. hardy rubber tree
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/12—Antihypertensives

Definitions

  • the present invention relates to a water extract fraction of Tochu Nakaba, and an ingestible composition, a pharmaceutical composition, a food composition, a medicine and a food containing the fraction. Furthermore, this invention relates to the manufacturing method of the said fraction.
  • Eucommia ulmoides oliver is a deciduous woody plant that is classified as a genus of the genus Eucommia from Central China, and has a height of 20m. Tonaka is different from the camellia plant, generally called tea, in that it contains no caffeine. Tochu Nakaba has been widely used as a beverage since the 1980s.
  • tea leaves for Tochu tea have been dried by sun-drying the tea leaves harvested in the production area, and then roasting the dried products, usually at 100-140 ° C for 30-50 minutes. However, it was necessary to boil for 10 minutes or more in order to perform sufficient water extraction on the tea leaves produced in this way. Subsequently, after steaming the nakanaka leaves, a method of subjecting them to a twisting process and a roasting process was disclosed, and hot water extraction of the nakanaka leaf components was made possible in a short time (see Patent Document 1).
  • tea leaves for ordinary Tochu tea change color from green to brown in processes such as sun drying and roasting, and the components contained in the tea leaves change accordingly.
  • a method for producing green Tochu tea leaves has also been developed in order to produce green Tochu tea and to contain more raw Tochu leaves in the extract (Patent Documents 2 to (See 4).
  • Patent Document 1 Japanese Patent Application Laid-Open No. 8-173110
  • Patent Document 2 Specification of Patent No. 2775418
  • Patent Document 3 JP-A-11-155537
  • Patent Document 4 JP-A-2005-287469
  • Patent Document 5 JP-A-2005-289950
  • Patent Document 6 JP-A-2005-289951
  • Patent Document 7 Japanese Patent Laid-Open No. 2003-342185
  • Patent Document 8 Japanese Patent Application Laid-Open No. 2002-179586
  • Patent Document 9 Japanese Patent Laid-Open No. 2002-275077
  • Tochu Nakaba As a beverage, a method for producing green Tochu tea leaves intended to retain raw leaf ingredients has been studied! In order to provide beverages, an extraction device or the like is required, and a method for preparing a water extract that can be used to provide Tochu tea more simply has been demanded. Furthermore, the water extract is required to have sufficient medicinal ingredients contained in Tochu Naka, to have a flavor similar to that of Tochu tea prepared by a usual method, and to have excellent storage stability. .
  • the obtained fraction is desired to be easy to handle and have long-term storage stability even when processed as a medicine or food.
  • the present invention provides a method for producing a fraction of a water extract that contains a sufficient amount of components of Tochu Naka, in particular, components of Tochu Tea, and has a flavor similar to that of Tochu Tea when dissolved in water; It is an object of the present invention to provide a fraction of a Tochu leaf ingredient that contains a high concentration of ingredients and is easy to handle during processing; and a beverage containing the fraction.
  • a production method including a step of adsorbing an extract of chunaka leaf water obtained from dried chunaka leaf on a synthetic adsorbent in reverse phase to obtain a component eluted with water.
  • a foliage extract of Tochu Nakahasui is provided.
  • the fraction obtained as a component eluted with water is concentrated. Then, it is provided with a fraction of the Nakanaka leaf water extract already defined as the present invention, which is obtained by a production method further comprising the step of obtaining a powder by drying under reduced pressure.
  • the powder is not particularly limited, but can be obtained, for example, by separating from the tar content.
  • the method for concentrating the fraction is not particularly limited, and can be carried out by methods such as distillation of water by heating under normal pressure and distillation of water under reduced pressure, for example, rotary evaporator. One or a freeze dryer may be used. In the present invention, for example, a certain amount of water is distilled off by heating under normal pressure, and then concentrated by using a rotary evaporator or the like.
  • the content of the asperal mouth side is 1% by weight or less based on the total amount of the solid formation.
  • the Tochu Nakaha extract fraction is provided.
  • the light absorption at 560nm (OD560) in a 0.3 wt% aqueous solution is not more than a certain value, and has already been defined as the present invention.
  • a product fraction is provided.
  • the absorbance may be, for example, 0.06 or less, preferably 0.058 or less, and more preferably 0.055 or less.
  • the absorbance is not particularly limited, but is immediately measured by dissolving the extract of Tochu Nakaba water in distilled water using a spectrophotometer UV-1200 manufactured by Shimadzu Corporation.
  • a production method including a step of drying tsunaka leaf by irradiating infrared rays is provided a tsunaka leaf water extract fraction already defined as the present invention.
  • a medicament comprising the fraction of the Nakanaka leaf water extract already defined as the present invention.
  • the Nakanaka leaf water extract already defined as the present invention A food product comprising the fraction is provided.
  • the food is not particularly limited, and may be, for example, a functional food, a health food, a health supplement, a nutritional supplement, an insurance function food, a specific insurance food, or a function nutrition food.
  • an aqueous solution of the fraction of the Nakanaka leaf water extract already defined as the present invention is provided.
  • the aqueous solution can be used as a beverage, for example.
  • the method includes the step of adsorbing an extract of chunaka leaf water obtained from dried chunaka leaf on a synthetic adsorbent of reverse phase to obtain a component eluted with water.
  • a method for producing a product fraction is provided.
  • the method further includes a step of concentrating the fraction obtained as a component eluted with water and drying under reduced pressure to obtain a powder.
  • the content of the asperal mouth side is 1% by weight or less with respect to the total amount of the solid formation.
  • a solid tsunaka leaf water extract fraction in which the content of the asperal mouth side is 1% by weight or less based on the total amount of the solid component.
  • the fraction of Tochu Nakaha extract contains a sufficient amount of Tochu tea components such as geposide acid and chlorogenic acid, and the aqueous solution has the same flavor as Tochu tea. Furthermore, since the fraction has preferable storage stability, it is useful as a raw material for beverages. In addition, the aqueous solution capable of obtaining the fractional power of the present invention has the characteristics of little change in color tone even when stored for a long period of time! The
  • fraction according to the present invention sufficiently contains the main components of Tochu tea, it is useful in the manufacture of pharmaceuticals and foods for efficiently ingesting the components.
  • the fraction of the present invention includes, for example, an iridoid compound that is a glycoside component contained in Tochu Nakaba.
  • iridoid compound that is a glycoside component contained in Tochu Nakaba.
  • Specific examples thereof include, for example, Geposide acid, Asper mouth side, Asper mouth mouth Examples include side acid (asperulosidic acid), deacetylase spell mouth side acid, scandoside 10 O acetate, oakbin and the like.
  • the chunaka leaf water extract in the present invention is prepared using, for example, dried chunaka leaf.
  • the dry licorice leaf is not particularly limited, but can be prepared, for example, by subjecting licorice leaf to a specific process.
  • the bamboo shoot leaves mean the bamboo leaves before harvest and before drying, and may be produced by cultivation or collected from nature. For example, fresh leaves before fallen leaves of the current year can be used, and fresh leaves can be used from April to October, preferably from May to August, more preferably from July to August.
  • the cocoon leaves of the present invention may be used as they are, or may be cut. Therefore, the present invention may further include a step of cutting the green leaves. ⁇ ⁇ may be cut into a width of, for example, about 5 to 30 mm, preferably about 10 to 20 mm. In the present invention, unbroken cocoon leaves are preferably used. By using the uncut green leaves, it becomes possible to dry gently and gently in the subsequent drying process, and it is possible to suppress the yield drop and the discoloration of the green leaves during the drying. .
  • the steaming step of the cocoon leaves in the present invention can be carried out by a method usually performed in the technical field using a commercially available steamer or an autoclave.
  • a commercially available steamer or an autoclave For example, it is possible to steam heat-treat cocoon leaves by spreading them on a net conveyor and passing them through a treatment chamber filled with non-pressure steam supplied from a boiler.
  • Kashimura Tekko Co., Ltd., leaf feeder, ground type 1500, net conveyor, trolley type 100, etc. can be used.
  • the steaming temperature may be appropriately selected within the range of, for example, 90 to 120 ° C., preferably 95 to 110 ° C., more preferably 100 to 110 ° C., depending on the size of Tochu Nakaba.
  • the steaming time can also be appropriately selected within the range of 10 to 240 seconds, preferably 20 to 180 seconds, more preferably 20 to 120 seconds.
  • the amount of steam used can be appropriately selected within the range of, for example, 200 to 70 LZ, preferably 170 to L00LZ.
  • the treatment amount of the steamed leaves can be appropriately selected in the range of, for example, 3 to: LO kgZ, preferably 4 to 8 kgZ, more preferably 5 to 7 kgZ, depending on the moisture content of the fresh leaves.
  • This steaming process makes it easier to maintain the green color of the Nakanaka leaf by inactivating the enzyme that changes the color of the Nakanaka leaf to brown;
  • the softening has the effect of facilitating the process of drying the steamed Nakanaka leaves after the steaming process with stirring and Z or pressure.
  • the steamed and heat-treated Tochu Nakaba can be used in the next step as it is. It can also be used in the next step after cooling.
  • the cooling here can be performed by removing rough heat by blowing air or the like.
  • the step of drying the bamboo leaf while stirring and Z or under pressure includes, for example, a commercially available continuous leaf cutter, rotary leaf cutter, and batch type leaf cutter. Alternatively, it can be carried out by a method commonly used in the technical field using a roughing machine or the like.
  • a commercially available leaf cutter leaf cutters (batch type) 60K, 90 ⁇ , 120K, and 180K manufactured by Kawasaki Kiko Co., Ltd. can be used.
  • the crushing pressure in this process is performed by a “more hand” supported by a panel panel having an appropriate elasticity rotating in the leaf cutter and pressing the corrugated leaf against the wall of the processing cylinder.
  • the stirring in this step is performed by rotating the “leaf crust” attached to the leaf cutter.
  • the rolling pressure in this step is preferably performed gently, for example, by only rotating the leaves.
  • the drying method is not particularly limited, but is preferably performed by sending hot air to Tochu Nakaba.
  • the temperature of the hot air is not particularly limited, but may be appropriately selected from the range of, for example, 70 to 120 ° C, preferably 75 to 110 ° C.
  • the time required for this step can be appropriately selected within the range of 10 to 60 minutes, preferably 15 to 50 minutes, more preferably 20 to 40 minutes. For example, this step can be performed at 100 ° C. for 20-30 minutes.
  • the surface of the nakanaka leaf can be maintained, the surface temperature of the nakanaka leaf can be kept within a certain range, and drying can be performed while suppressing discoloration of the nakanaka leaf. It becomes possible.
  • the surface temperature of Tochu tea leaves during drying in this step is, for example, 30 to 60 ° C, preferably 40 to 50 ° C. is there.
  • the water content of Tochu Nakaba obtained through this step is, for example, 30 to 45%, preferably 30 to 40%, more preferably 30 to 35% on a dry basis.
  • the present invention includes a step of homogenizing the moisture of the chunaka leaf between "the step of drying the chunaka leaf with stirring and Z or pressure" and "the step of drying the chunaka leaf". It may be.
  • the step of homogenizing the water in the bamboo leaf can be carried out by a method usually performed in the technical field using, for example, a commercially available twister, coarser, or intermediater.
  • a commercially available twister Terada Seisakusho Co., Ltd.
  • a twister 60 kg type can be used as a commercially available twister, Terada Seisakusho Co., Ltd.
  • the homogenization of the water in the cocoon leaf in this process is performed, for example, by sandwiching the cocoon leaf between the turret and the rotating drum of the turret and pressurizing with the rotator while rotating the rotator. Is called. Since the Tochu leaves are dried in the order of mesophyll, vein, and leaf shaft, for example, even if the dryness of the leaf meat is sufficient, excess moisture still remains on the leaf shaft. Therefore, according to this step, the moisture in the bamboo leaf is uniformly prepared, and the powdery wrinkles due to partial drying are suppressed, and at the same time, the time required for drying can be shortened. This step can be performed with heating as required, preferably without heating.
  • the time required for this step can be appropriately selected within the range of 10 to 80 minutes, preferably 20 to 60 minutes, more preferably 30 to 45 minutes.
  • this process can be performed at room temperature for 40 minutes.
  • the force applied by the scissors during this process The no-pressing time at the start of the process can be appropriately selected in the range of 0 to 10 minutes, preferably 2 to 8 minutes, more preferably 4 to 5 minutes. .
  • the moisture content of the chunaka leaf obtained through this step is, for example, 25 to 40%, preferably 25 to 35% on a dry basis.
  • the present invention includes "a step of homogenizing moisture in the chunaka leaf”, an additional “step of drying the chunaka leaf with stirring and Z or under pressure before the step of drying the chunaka leaf. And “the step of homogenizing the water in the Tochu leaf”, or “the step of drying the Tochu leaf with stirring and Z or pressure” may be included. By repeating these steps, it is possible to obtain Tochu tea leaves from which unnecessary lipids have been further removed.
  • the step can be performed by the above-described method and conditions, and the temperature of the hot air is not particularly limited.
  • 50 to 110 A range force of ° C, preferably 55-105 ° C can also be selected as appropriate.
  • the time required for the step can be appropriately selected within the range of 5 to 45 minutes, preferably 10 to 40 minutes, more preferably 10 to 35 minutes. For example, it can be performed at 70 ° C for 20 to 30 minutes.
  • the step can be performed by the above-mentioned method and conditions, and the time required for this step is 10 to 80 minutes, preferably It can be appropriately selected within a range of 20 to 60 minutes, more preferably 30 to 45 minutes. For example, it can be performed at room temperature for 40 minutes.
  • the step of drying the Tochu leaf of the present invention can be carried out, for example, by a method commonly performed in the technical field using a commercially available dryer.
  • the drying method in this process is not specifically limited, For example, it can carry out by moving the Tochu Naka leaf on a conveyance conveyor in the high temperature drying chamber filled with the hot air by a hot air generator.
  • a hot air generator For example, it can be performed by a dryer ND120 manufactured by Terada Manufacturing Co., Ltd.
  • the temperature of the hot air is not particularly limited, but a range force of, for example, 70 to 100 ° C., preferably 85 to 95 ° C. may be appropriately selected.
  • the time required for this step can be appropriately selected within the range of 5 to 80 minutes, preferably 10 to 70 minutes, more preferably 20 to 80 minutes.
  • the water content of the bamboo leaf obtained through this step is, for example, a water content of 5% or less, preferably a water content of 3% or less, more preferably a water content of 2% or less.
  • the present invention may include a step of drying the chunaka leaf by irradiating the chunaka leaf with far infrared rays.
  • this process is not specifically limited, For example, it can be performed using the commercially available far-infrared heater.
  • it can be performed by a VR type manufactured by Yamamasu Seisakusho Co., Ltd.
  • the wavelength of the far infrared rays irradiated here is appropriately selected from the range of, for example, 1 to: LOOO / z m, preferably 2.5 to 50 ⁇ m, more preferably 3 to 30 ⁇ m.
  • the irradiation set temperature during drying can be appropriately selected from the range of, for example, 100 to 400 ° C, preferably 150 to 350 ° C, more preferably 200 to 300 ° C.
  • the time required for this step can be appropriately selected within the range of 30 to 60 seconds, preferably 40 to 55 seconds, and more preferably 45 to 50 seconds as the infrared lamp passing speed.
  • the moisture content of the dried bamboo leaf obtained through this step is, for example, a moisture content of 5% or less, preferably a moisture content of 3% or less, more preferably a moisture content of 2% or less.
  • the Tochu Nakaba water extract is, for example, 5 to 50 k with respect to 1 kg of dried Tochu Nakaba. g, preferably 10-30 kg, more preferably 15-20 kg force
  • An appropriately selected amount of water can be used.
  • the extraction temperature can be appropriately selected from the range of, for example, 85 to 98 ° C, preferably 90 to 95 ° C.
  • the extraction time is not particularly limited, but can be appropriately selected, for example, 10 to 120 minutes, preferably 20 to 90 minutes, more preferably 30 to 60 minutes.
  • Filtration of the extract may be performed using, for example, a filter or a 30-200 mesh filter.
  • the filtrate may be allowed to stand for a certain period before concentration. Unnecessary substances can be removed by removing precipitates generated by standing.
  • the standing time is not particularly limited, but may be appropriately selected from, for example, 1 to 24 hours, preferably 6 to 20 hours, and more preferably 8 to 18 hours.
  • the temperature at the time of standing is not particularly limited. For example, a force of 0 to 35 ° C, preferably 0 to 16 ° C, more preferably 2 to 8 ° C can be appropriately selected.
  • the soot extract obtained in this way can be used as it is, but the supernatant obtained after removing the precipitate generated by centrifugation can also be used in the next step. Further, the supernatant can be used after being concentrated.
  • the "reverse-phase synthetic adsorbent" used in the present invention is not particularly limited as long as it is usually used for separation of natural components, etc., but polystyrene gel (polystyrene 'dibulene benzene copolymer, etc.)
  • polystyrene gel polystyrene 'dibulene benzene copolymer, etc.
  • MCIGEL CHP10M / CHP2MGM SEPABEA
  • the adsorbent can be used as a column carrier, for example.
  • the amount of adsorbent used can be selected, for example, in the range of 10000 to 18000 g, preferably ⁇ 15000 to 18000 g, for 110 L of Tochu leaf extract.
  • a Tochu leaf extract is injected into a column packed with a reverse-phase adsorbent, and then a component that is eluted using a fixed amount of water as an eluent is separated into the components of the present invention. It can be obtained as a picture.
  • the amount of water to be used as the eluent is not particularly limited.
  • a range force of 70 to L 10 L can be used for 17200 g of the adsorbent.
  • the content of the asperal mouth side in the solid component after the solvent is distilled off is, for example, HPLC And the like (the measurement wavelength of absorbance: 215 nm).
  • the content of aucubin (absorbance measurement wavelength: 215 nm), chlorogenic acid (absorbance measurement wavelength: 215 nm) and geposide acid (absorbance measurement wavelength: 215 nm) should be determined in the same manner. Can do.
  • the fraction of the present invention can be obtained as a powder.
  • the content of asperuloside contained in the powder is 1% by weight or less based on the total amount of solid components after the solvent is distilled off. An example is 0.01% by weight or less.
  • the content of Aukubin contained in the powder is 15.0 to 18 against the solid component amount of After evaporation of the solvent. 0 weight 0/0, preferably 16.0 to 18.0 weight 0/0, more preferably 16.4 to 17.4 wt%. An example is 16.9% by weight.
  • the content of chlorogenic acids contained in the powder is from 5.61 to 6.85 wt 0/0 relative to the solid component amount of After evaporation of the solvent, preferably from 5.92 to 6. 54 weight 0/0, more preferably
  • the content of Poshido acid is from 6.50 to 7 94 weight 0/0 relative to the solid component amount of After evaporation of the solvent, preferably 6. 86 to 7.58 weight 0/0, and more preferably
  • the total content of alkvin, chlorogenic acid, and geposide acid contained in the powder is 27.11-32.79 wt% with respect to the total amount of solid components after the solvent is distilled off, Preferably 2
  • the fraction of the present invention can be obtained as a tar-like product.
  • the content of the asper mouth side contained in the tar-like product is 1% by weight or less with respect to the total amount of solid components after the solvent is distilled off, and an example is 0.01% by weight or less.
  • the content of alkvin contained in the tar-like product is 13.5-16. 5% by weight, preferably 14.3-15.8% by weight based on the total amount of solid components after the solvent is distilled off. 0/0, more rather preferably is 14. 6-15. 4 wt%. An example is 15.0% by weight.
  • the content of chlorogenic acids contained in the tar-like product is 14 to 6.28 wt 0/0 5. to solid formation amount total after evaporation of the solvent, preferably 5. 42 to 6.00 weight 0/0, more virtuous It is preferably 5.54-5.88% by weight. An example is 5.7% by weight.
  • gate contained in the tar-like product - The content of Poshido acid is 5. 90-7 22 weight 0/0 to solid formation amount total after evaporation of the solvent, preferably 6. 23-6. 89 weight 0/0, which is the more favorable Mashiku 6. 36-6. 76 wt%. An example is 6.6% by weight.
  • the total amount of alkvin, chlorogenic acid, and geoposide acid contained in the tar-like product is 24.54-300.0% by weight based on the total amount of solid components after the solvent is distilled off, preferably from 25.95 to 28.69 wt 0/0, more preferably from 26.50 to 28.04 wt 0/0.
  • An example is 27.3% by weight.
  • the fraction obtained as an aqueous solution is preferably used after being concentrated.
  • the concentration rate is not particularly limited, but is, for example, 60% or less in volume ratio, preferably 30% or less, and more preferably used as a solid product obtained by drying.
  • the concentration can be performed under normal pressure or reduced pressure, preferably under reduced pressure.
  • the concentration method is not particularly limited as long as it is a commonly used method, and for example, it can be concentrated using a rotary evaporator.
  • the fraction of the present invention can be used as an active ingredient of a pharmaceutical composition having a medicinal effect such as blood pressure lowering or lipase inhibition.
  • the pharmaceutical composition can be used in various dosage forms such as tablets, capsules, powders, granules, pills, solutions, emulsions, suspensions, solutions, spirits, syrups, for oral administration. Extracts, elixirs, and the like can be used. For example, for topical administration, creams, jellies, gels, pastes, ointments, and the like can be used.
  • the pharmaceutical composition may contain various commonly used ingredients, for example, one or more pharmaceutically acceptable excipients, disintegrants, diluents, lubricants, flavors.
  • compositions of the present invention may be in a sustained or sustained release dosage form.
  • the fraction of the present invention can be used, for example, as a component of a tablet.
  • the tablet is produced by a production method usually used in the field of pharmaceutical preparations, and for example, supplemental carotenants such as crystalline cellulose, silicon dioxide, sucrose fatty acid ester, yeast cell wall, glycerin and the like can be used.
  • excipients for example, maltose, dextrin, cyclodex (String, agar, corn protein, tricalcium phosphate, carboxymethylcellulose calcium, vegetable oil, glycerin fatty acid ester, gum arabic
  • the fraction of the present invention preferably contains an excipient.
  • the tablet can be used as a component of the tablet.
  • the tablet is produced by a production method usually used in the field of pharmaceutical preparations.
  • additives such as crystalline cellulose, diacid carbonate, sucrose fatty acid ester, yeast cell wall, and glycerin can be used. .
  • the fraction of the present invention can be used as a powder. Since the powder is granular, it has low viscosity and low hygroscopicity, so that it can be easily encapsulated. In addition, the powder is less likely to be welded or solidified after the packaging material is enclosed. Maintains granularity even at high temperatures (35 ° C) for a long time, for example, 2 weeks.
  • the dose of the anti-obesity agent of the present invention can be appropriately selected depending on the patient's body shape, age, physical condition, degree of disease, elapsed time after onset, etc.
  • the pharmaceutical composition of the present invention is a therapeutic agent.
  • An effective amount and Z or a prophylactically effective amount of an anti-obesity agent can be included.
  • it is generally used in the dose of 10 to 50000 mg / day / adult, preferably 100 to 5000 mg Z day Z adult, as the pulverized mash of the present invention or its water extract.
  • Administration of the pharmaceutical composition can be single dose or multiple doses and can be used in combination with other drugs such as other anti-obesity agents.
  • the food according to the present invention includes a liquid beverage and a solid food.
  • the food can be used as quasi-drugs, other food and drink ingredients, food additives, and the like.
  • the composition for ingestion in this specification can be used as a functional food as it is, and can be used as a component of a pharmaceutical, a quasi-drug, a food and drink, and a food additive.
  • the use enables daily and continuous ingestion of the food or composition for oral consumption having the obesity-improving effect and arteriosclerosis-preventing agent of the present invention, effectively improving obesity, and obesity-related diseases. (Eg hypertension, hyperlipidemia, diabetes) Effective prevention is possible.
  • Examples of foods or beverages containing the anti-obesity agent of the present invention include functional foods, health foods, health supplements, nutritional supplements (nutrient drinks) having a hypotensive effect, a lipase inhibitory effect or an obesity inhibitory effect, etc. Etc.), functional insurance food, food for specified insurance, functional nutrition food, general food (juice, confectionery, processed food, etc.).
  • the food or beverage in this specification includes, as optional additives, inorganic components such as iron and calcium, various vitamins, dietary fibers such as oligosaccharides and chitosan, proteins such as soy extract, and lecithin. Sugars such as lipids, sucrose and lactose can be included.
  • Ion-exchanged water 70 L was added to dry potato Nakaba (10 kg) and heated at 65 ° C for 7 hours. After heating, it was left overnight.
  • Use Zaru Tetron No. 3 to remove the residue from the Nakanaka leaf, and then perform continuous centrifugation! ⁇ (centrifugal acceleration: 9340xg; S-type ultra-high speed centrifuge U— 6— H: Kansai Enshin Separator ), And a supernatant (about 55 L) was obtained.
  • Another batch was performed in the same manner, and the Tochu leaf extract was combined to obtain a solution of about 110 L.
  • the obtained solution was poured into a column (diameter 135x length 1200mm) packed with Diaion HP20.
  • the aqueous solution that also eluted the column force was collected, and then further 70 L of water was used to elute with water.
  • the obtained aqueous solution was concentrated at 65 ° C under reduced pressure (0.5 kPa), and dried to remove the powder generated on the surface of the tar-like material each time.
  • powder fraction A (1930. Og) and tar-like fraction B (2590. Og) were obtained.
  • the obtained solution was concentrated under reduced pressure (0.5 kPa) to 65 ° C until dryness, to obtain a dried kashiwa leaf water extract (652g) as a solid.
  • test solution 1.5 g each of dried kashiwa leaf extract, fraction A and fraction B were weighed, adjusted to 500 g by adding distilled water, and stirred to obtain a test solution.
  • the test solution was sealed in a transparent sealed container and stored at 40 ° C for 2 weeks.
  • the test solution was evaluated by measuring the absorbance at a wavelength of OD560 and OD720 using a visual evaluation of the test solution and a spectrophotometer (UV-1200: manufactured by Shimadzu Corporation).
  • Example 3 The test solution of Example 3 was used as the test solution.
  • the viscosity of the test solution was measured using a digital viscometer (DV— ⁇ +: manufactured by Brook Field) with an ultra-low viscosity UL adapter.
  • Example 3 The test solution of Example 3 was used as the test solution.
  • the taste evaluation of the test solution was measured using a taste sensor 1 (taste recognition device SA402B: manufactured by Incent Corporation).
  • Example 3 The test solution of Example 3 was used as the test solution.
  • the components contained in the test solution were a ucubin, asperuloside, geniposidic acid and chlorogenic acid as standard substances.
  • FIG. 1 is a diagram showing an example of a taste test result of an aqueous solution of Tochu Nakaha water extract.

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Abstract

[PROBLEMS] To provide: a fractionation method for extracting an ingredient, particularly a pharmacologically active ingredient such as an iridoid compound, from an Eucommia ulmoides oliver leaf with good efficiency; a fractionation product of an Eucommia ulmoides oliver leaf, wherein the fractionation product contains a specific component in a large concentration; and a composition for oral ingestion, pharmaceutical composition, food composition, pharmaceutical or food which comprises a natural material as a raw material, produces little adverse side effects, and is safe even when ingested over a long period. [MEANS FOR SOLVING PROBLEMS] Disclosed is a fractionation product of an aqueous extract of an Eucommia ulmoides oliver leaf. The fractionation product can be produced by a process comprising the step of adsorbing an aqueous extract of an Eucommia ulmoides oliver leaf onto a reverse-phase synthetic adsorbent to yield a component which can be eluted with water, wherein the aqueous extract is prepared from a dried Eucommia ulmoides oliver leaf. Also disclosed is a pharmaceutical or food comprising the fractionation product.

Description

明 細 書  Specification
杜仲葉水抽出物の分画物、およびそれを含む経口摂取用組成物 技術分野  A fraction of Tochu Nakaha extract and composition for oral intake containing the same
[0001] 本発明は、杜仲葉の水抽出物分画物、および当該分画物を含む経口摂取用組成 物、医薬組成物、食品組成物、医薬および食品に関する。さらに本発明は、当該分 画物の製造方法に関する。  [0001] The present invention relates to a water extract fraction of Tochu Nakaba, and an ingestible composition, a pharmaceutical composition, a food composition, a medicine and a food containing the fraction. Furthermore, this invention relates to the manufacturing method of the said fraction.
背景技術  Background art
[0002] 杜仲（Eucommia ulmoides oliver)は、中国中央部起源のトチュウ科トチュウ属のー 科ー属一種に分類される落葉性木本類で、榭高が 20mに達する喬木である。杜仲 は、一般にお茶と称するツバキ科の植物と比較し、カフェインを全く含んでいないほ 力 含有物も異なる。杜仲葉は、 1980年代から飲料としての用途が普及している。  [0002] Eucommia ulmoides oliver is a deciduous woody plant that is classified as a genus of the genus Eucommia from Central China, and has a height of 20m. Tonaka is different from the camellia plant, generally called tea, in that it contains no caffeine. Tochu Nakaba has been widely used as a beverage since the 1980s.
[0003] 杜仲茶用の茶葉の製造は、従来は、産地で収穫した茶葉を天日乾燥し、この乾燥 品を通常 100〜140°Cで 30から 50分間焙煎し、さらに適当な大きさに切断すること により行われて 、たが、このようにして製造された茶葉にぉ 、て十分な水抽出を行う ためには、 10分以上の煮沸が必要であった。その後、杜仲生葉を蒸熱した後に、揉 捻工程、焙煎工程に付す方法などが開示され、短時間での杜仲葉成分の熱水抽出 が可能となった (特許文献 1を参照)。  [0003] Traditionally, tea leaves for Tochu tea have been dried by sun-drying the tea leaves harvested in the production area, and then roasting the dried products, usually at 100-140 ° C for 30-50 minutes. However, it was necessary to boil for 10 minutes or more in order to perform sufficient water extraction on the tea leaves produced in this way. Subsequently, after steaming the nakanaka leaves, a method of subjecting them to a twisting process and a roasting process was disclosed, and hot water extraction of the nakanaka leaf components was made possible in a short time (see Patent Document 1).
[0004] また、通常の杜仲茶用の茶葉は、天日乾燥および焙煎などの工程において緑色か ら茶褐色に変色し、それに伴って茶葉に含まれる成分も変化する。一方、緑色の杜 仲茶を製造するため、および生の杜仲葉の成分をより多く抽出液に含ませるために、 緑色の杜仲茶葉を製造するための方法も開発されている (特許文献 2〜4を参照)。  [0004] In addition, tea leaves for ordinary Tochu tea change color from green to brown in processes such as sun drying and roasting, and the components contained in the tea leaves change accordingly. On the other hand, a method for producing green Tochu tea leaves has also been developed in order to produce green Tochu tea and to contain more raw Tochu leaves in the extract (Patent Documents 2 to (See 4).
[0005] 天然物由来の食品や漢方薬は、一般に副作用が少ないなどの利点を有することか ら、近年において発生が増加している生活習慣病に対してのその有用性が注目され ている。上述の杜仲に関しても、杜仲葉の成分のリパーゼ阻害活性について検討し た例も 、くつかの報告がされて 、る（特許文献 5〜9を参照)。  [0005] Foods derived from natural products and herbal medicines have advantages such as generally having few side effects, and thus their usefulness against lifestyle-related diseases, which have been increasing in recent years, has attracted attention. As for the above-mentioned Tochu, there have been several reports on the investigation of the lipase inhibitory activity of Tonaka leaf components (see Patent Documents 5 to 9).
[0006] 特許文献 1 :特開平 8— 173110号公報  Patent Document 1: Japanese Patent Application Laid-Open No. 8-173110
特許文献 2：特許 2775418号明細書 特許文献 3 :：特開平 11 - - 155537号公報 Patent Document 2: Specification of Patent No. 2775418 Patent Document 3: JP-A-11-155537
特許文献 4: ：特開 2005- — 287469号公報  Patent Document 4:: JP-A-2005-287469
特許文献 5 : ：特開 2005- — 289950号公報  Patent Document 5:: JP-A-2005-289950
特許文献 6 : ：特開 2005- — 289951号公報  Patent Document 6:: JP-A-2005-289951
特許文献 7 : ：特開 2003- — 342185号公報  Patent Document 7:: Japanese Patent Laid-Open No. 2003-342185
特許文献 8 : ：特開 2002- — 179586号公報  Patent Document 8:: Japanese Patent Application Laid-Open No. 2002-179586
特許文献 9 : ：特開 2002- - 275077号公報  Patent Document 9:: Japanese Patent Laid-Open No. 2002-275077
発明の開示  Disclosure of the invention
発明が解決しょうとする課題  Problems to be solved by the invention
[0007] 杜仲葉の飲料としての使用につ 、ては、生葉の成分を保持させることを意図した緑 色の杜仲茶葉を製造方法が検討されて!ヽるが、杜仲葉カゝら抽出して飲料を供するた めには抽出用器具などが必要となり、より簡便に杜仲茶を供するために使用すること ができる水抽出物の調製方法が求められていた。さらに、当該水抽出物については 、杜仲葉に含まれる薬効成分を十分に含むと共に、通常の方法で調製した杜仲茶と 同程度の風味を有し、かつ優れた保存性を有することが求められる。  [0007] With regard to the use of Tochu Nakaba as a beverage, a method for producing green Tochu tea leaves intended to retain raw leaf ingredients has been studied! In order to provide beverages, an extraction device or the like is required, and a method for preparing a water extract that can be used to provide Tochu tea more simply has been demanded. Furthermore, the water extract is required to have sufficient medicinal ingredients contained in Tochu Naka, to have a flavor similar to that of Tochu tea prepared by a usual method, and to have excellent storage stability. .
[0008] 得られた分画物は、医薬または食品としての加工時においても、取り扱いが容易で 長期保存性を有することが望まれて 、る。  [0008] The obtained fraction is desired to be easy to handle and have long-term storage stability even when processed as a medicine or food.
したがって、本発明は、杜仲葉の成分、特に杜仲茶の成分を十分に含み、水に溶 解した際に杜仲茶と同様の風味を有する水抽出物の分画物の製造方法;特定の成 分を高濃度で含有し加工時の取り扱いが容易な杜仲葉成分の分画物;および当該 分画物を含む飲料を提供することを目的とする。  Therefore, the present invention provides a method for producing a fraction of a water extract that contains a sufficient amount of components of Tochu Naka, in particular, components of Tochu Tea, and has a flavor similar to that of Tochu Tea when dissolved in water; It is an object of the present invention to provide a fraction of a Tochu leaf ingredient that contains a high concentration of ingredients and is easy to handle during processing; and a beverage containing the fraction.
課題を解決するための手段  Means for solving the problem
[0009] 本発明者は、上記の課題解決のために鋭意研究を進めたところ、杜仲葉成分の特 定の分画物に、好ましい特性を見いだし、本発明を完成させた。 [0009] As a result of diligent research aimed at solving the above-mentioned problems, the present inventor has found preferable characteristics for a specific fraction of Tochu Nakaba ingredients and completed the present invention.
すなわち本発明の一つの側面によれば、乾燥杜仲葉から得られる杜仲葉水抽出物 を逆相の合成吸着剤に吸着させ、水で溶出する成分を得る工程を含む製造方法に より得られる、杜仲葉水抽出物分画物が提供される。  That is, according to one aspect of the present invention, it can be obtained by a production method including a step of adsorbing an extract of chunaka leaf water obtained from dried chunaka leaf on a synthetic adsorbent in reverse phase to obtain a component eluted with water. A foliage extract of Tochu Nakahasui is provided.
[0010] 本発明の別の側面によれば、前記水で溶出する成分として得られる分画物を濃縮 し、減圧乾燥し、粉体を得る工程をさらに含む製造方法により得られる、本発明として 既に定義した杜仲葉水抽出物分画物が提供される。ここで当該粉体は、特には限定 されないが、例えばタール分と分離することにより得ることができる。分画物の濃縮方 法は、特には限定されず、例えば、常圧下での加熱による水分の留去、および減圧 下での水分の留去などの方法により行うことができ、例えば、ロータリーエバポレータ 一や凍結乾燥機などを使用してもよい。本発明においては、例えば、常圧下で加熱 することによりある程度の水分の留去し、その後、ロータリーエバポレーターなどを使 用してさら〖こ濃縮することちでさる。 [0010] According to another aspect of the present invention, the fraction obtained as a component eluted with water is concentrated. Then, it is provided with a fraction of the Nakanaka leaf water extract already defined as the present invention, which is obtained by a production method further comprising the step of obtaining a powder by drying under reduced pressure. Here, the powder is not particularly limited, but can be obtained, for example, by separating from the tar content. The method for concentrating the fraction is not particularly limited, and can be carried out by methods such as distillation of water by heating under normal pressure and distillation of water under reduced pressure, for example, rotary evaporator. One or a freeze dryer may be used. In the present invention, for example, a certain amount of water is distilled off by heating under normal pressure, and then concentrated by using a rotary evaporator or the like.
[0011] さらに、本発明の別の側面によれば、溶媒を留去した後の固形成分として、当該固 形成分の総量に対しァスペル口サイドの含有量が 1重量％以下である、本発明として 既に定義した杜仲葉水抽出物分画物が提供される。  [0011] Further, according to another aspect of the present invention, as the solid component after distilling off the solvent, the content of the asperal mouth side is 1% by weight or less based on the total amount of the solid formation. As provided, the Tochu Nakaha extract fraction is provided.
[0012] さらに、本発明の別の側面によれば、 0. 3重量％水溶液としたときの 560nmにお ける吸光度 (OD560)が一定値以下である、本発明として既に定義した杜仲葉水抽 出物分画物が提供される。ここで、前記吸光度は、例えば 0. 06以下、好ましくは 0. 058以下、より好ましくは 0. 055以下であってもよい。なお、吸光度は、特に限定はさ れないが株式会社島津製作所製、分光光度計 UV— 1200により、杜仲葉水抽出物 分画物を蒸留水に溶解させ、直ちに測定される。  [0012] Further, according to another aspect of the present invention, the light absorption at 560nm (OD560) in a 0.3 wt% aqueous solution is not more than a certain value, and has already been defined as the present invention. A product fraction is provided. Here, the absorbance may be, for example, 0.06 or less, preferably 0.058 or less, and more preferably 0.055 or less. The absorbance is not particularly limited, but is immediately measured by dissolving the extract of Tochu Nakaba water in distilled water using a spectrophotometer UV-1200 manufactured by Shimadzu Corporation.
[0013] さらに、本発明の別の側面によれば、前記乾燥杜仲葉が、杜仲生葉を蒸熱するェ 程;杜仲葉を攪拌および Zまたは揉圧しながら乾燥する工程;および杜仲葉に対して 遠赤外線を照射することにより杜仲葉を乾燥する工程を含む製造方法により得られる 、本発明として既に定義した杜仲葉水抽出物分画物が提供される。  [0013] Further, according to another aspect of the present invention, the step of steaming the dried nakanaka leaves; the step of drying the nakanaka leaves with stirring and Z or pressure; Provided by a production method including a step of drying tsunaka leaf by irradiating infrared rays is provided a tsunaka leaf water extract fraction already defined as the present invention.
[0014] さらに、本発明の別の側面によれば、錠剤の成分として使用される、本発明として 既に定義した杜仲葉水抽出物分画物が提供される。  [0014] Further, according to another aspect of the present invention, there is provided a fraction of the extract of Tochu-nakaba water, which has already been defined as the present invention, to be used as a component of a tablet.
さらに、本発明の別の側面によれば、本発明として既に定義した杜仲葉水抽出物 分画物を含む医薬が提供される。  Furthermore, according to another aspect of the present invention, there is provided a medicament comprising the fraction of the Nakanaka leaf water extract already defined as the present invention.
[0015] さらに、本発明の別の側面によれば、水に溶解して飲料として使用される、本発明 として既に定義した杜仲葉水抽出物分画物が提供される。 [0015] Further, according to another aspect of the present invention, there is provided a fraction of the Nakanaka leaf water extract already defined as the present invention, which is used as a beverage after being dissolved in water.
さらに、本発明の別の側面によれば、本発明として既に定義した杜仲葉水抽出物 分画物を含む食品が提供される。ここで、当該食品は、特には限定されないが、例え ば、機能性食品、健康食品、健康補助食品、栄養補助食品、保険機能食品、特定 保険用食品または栄養機能食品であってもよい。 Furthermore, according to another aspect of the present invention, the Nakanaka leaf water extract already defined as the present invention A food product comprising the fraction is provided. Here, the food is not particularly limited, and may be, for example, a functional food, a health food, a health supplement, a nutritional supplement, an insurance function food, a specific insurance food, or a function nutrition food.
[0016] さらに、本発明の別の側面によれば、本発明として既に定義した杜仲葉水抽出物 分画物の水溶液が提供される。当該水溶液は、例えば、飲料として使用されうる。 さらに、本発明の別の側面によれば、乾燥杜仲葉から得られる杜仲葉水抽出物を 逆相の合成吸着剤に吸着させ、水で溶出する成分を得る工程を含む、杜仲葉水抽 出物の分画物の製造方法が提供される。  [0016] Furthermore, according to another aspect of the present invention, there is provided an aqueous solution of the fraction of the Nakanaka leaf water extract already defined as the present invention. The aqueous solution can be used as a beverage, for example. Furthermore, according to another aspect of the present invention, the method includes the step of adsorbing an extract of chunaka leaf water obtained from dried chunaka leaf on a synthetic adsorbent of reverse phase to obtain a component eluted with water. A method for producing a product fraction is provided.
[0017] さらに、本発明の別の側面によれば、前記水で溶出する成分として得られる分画物 を濃縮し、減圧乾燥し、粉体を得る工程をさらに含む、本発明として既に定義した杜 仲葉水抽出物分画物の製造方法が提供される。 [0017] Further, according to another aspect of the present invention, the method further includes a step of concentrating the fraction obtained as a component eluted with water and drying under reduced pressure to obtain a powder.仲 Provided is a method for producing a Nakaba water extract fraction.
[0018] さらに、本発明の別の側面によれば、溶媒を留去した後の固形成分として、当該固 形成分の総量に対しァスペル口サイドの含有量が 1重量％以下である、本発明として 既に定義した杜仲葉水抽出物分画物の製造方法が提供される。 [0018] Further, according to another aspect of the present invention, as the solid component after the solvent is distilled off, the content of the asperal mouth side is 1% by weight or less with respect to the total amount of the solid formation. A process for producing a fraction of the Tochu Nakaha extract already defined as is provided.
[0019] さらに、本発明の別の側面によれば、ァスペル口サイドの含有量が当該固形成分の 総量に対し 1重量％以下である、固体の杜仲葉水抽出物分画物が提供される。 発明の効果 [0019] Furthermore, according to another aspect of the present invention, there is provided a solid tsunaka leaf water extract fraction in which the content of the asperal mouth side is 1% by weight or less based on the total amount of the solid component. . The invention's effect
[0020] 本発明により、杜仲葉水抽出物の分画物は、ゲ-ポシド酸、クロロゲン酸などの杜 仲茶の成分を十分に含み、さらにその水溶液は杜仲茶と同様の風味を有する。さら に、当該分画物は好ましい貯蔵安定性を有するため、飲料の原料として有用である。 また、本発明の分画物力も得られる水溶液は、長時間保存した場合にも色調の変化 が少な!/、と!/、う特徴を有し、飲料として好ま U、性質を有して 、る。  [0020] According to the present invention, the fraction of Tochu Nakaha extract contains a sufficient amount of Tochu tea components such as geposide acid and chlorogenic acid, and the aqueous solution has the same flavor as Tochu tea. Furthermore, since the fraction has preferable storage stability, it is useful as a raw material for beverages. In addition, the aqueous solution capable of obtaining the fractional power of the present invention has the characteristics of little change in color tone even when stored for a long period of time! The
[0021] さらに本発明に係る分画物は、杜仲茶の主な成分を十分に含むため、当該成分を 効率よく摂取するための医薬、食品の製造において有用である。  [0021] Furthermore, since the fraction according to the present invention sufficiently contains the main components of Tochu tea, it is useful in the manufacture of pharmaceuticals and foods for efficiently ingesting the components.
本発明の具体的な態様  Specific embodiments of the present invention
[0022] 以下、本発明をさらに具体的に説明する。  [0022] Hereinafter, the present invention will be described more specifically.
本発明の分画物には、例えば杜仲葉に含まれる配糖体成分であるイリドイドィ匕合物 が含まれる。その具体例には、例えば、ゲ-ポシド酸、ァスペル口サイド、ァスペル口 サイド酸（asperulosidic acid)、デァセチルァスペル口サイド酸、スカンドサイド 10 O アセテート、オークビンなどが含まれる。 The fraction of the present invention includes, for example, an iridoid compound that is a glycoside component contained in Tochu Nakaba. Specific examples thereof include, for example, Geposide acid, Asper mouth side, Asper mouth mouth Examples include side acid (asperulosidic acid), deacetylase spell mouth side acid, scandoside 10 O acetate, oakbin and the like.
[0023] 本発明における杜仲葉水抽出物は、例えば乾燥杜仲葉を使用して調製される。当 該乾燥杜仲葉は、特には限定されないが、例えば杜仲生葉を特定の工程に付すこと により調製されうる。ここで、杜仲生葉は、収穫後乾燥前の杜仲葉を意味するもので あり、栽培により生産されたものであっても天然より採取されたものであってもよい。例 えば、当年葉で落葉前の生葉を用い、採取時期は 4月から 10月、好ましくは 5月から 8月、より好ましくは 7月力 8月までの生葉を用いることができる。  [0023] The chunaka leaf water extract in the present invention is prepared using, for example, dried chunaka leaf. The dry licorice leaf is not particularly limited, but can be prepared, for example, by subjecting licorice leaf to a specific process. Here, the bamboo shoot leaves mean the bamboo leaves before harvest and before drying, and may be produced by cultivation or collected from nature. For example, fresh leaves before fallen leaves of the current year can be used, and fresh leaves can be used from April to October, preferably from May to August, more preferably from July to August.
[0024] 本発明の杜仲生葉は、そのまま用いてもよいが、裁断したものを用いてもよい。従つ て、本願発明は、杜仲生葉を裁断する工程をさらに含んでいてもよい。杜仲生葉は、 例えば 5〜30mm程度、好ましくは 10〜20mm程度の幅に切断して用いてもよい。 好ましくは、本発明においては裁断されていない杜仲生葉が用いられる。裁断されて いない杜仲生葉を利用することにより、その後の乾燥工程において、緩や力な乾燥 が可能となり、乾燥中に葉が崩れることによる歩留まりの低下、および杜仲葉の変色 を抑制することができる。  [0024] The cocoon leaves of the present invention may be used as they are, or may be cut. Therefore, the present invention may further include a step of cutting the green leaves.杜中生 葉 may be cut into a width of, for example, about 5 to 30 mm, preferably about 10 to 20 mm. In the present invention, unbroken cocoon leaves are preferably used. By using the uncut green leaves, it becomes possible to dry gently and gently in the subsequent drying process, and it is possible to suppress the yield drop and the discoloration of the green leaves during the drying. .
[0025] 本発明における杜仲生葉の蒸熱工程は、市販されて!ヽる蒸し機またはオートクレー ブなどを用いて、当該技術分野で通常行われている方法により実施することができる 。例えば、ネットコンベア上に杜仲生葉を広げ、ボイラーから供給される無圧蒸気を 充満させた処理室を通過させることにより、杜仲生葉を蒸熱処理することができる。例 えば、宫村鉄工株式会社製、給葉機、地上型 1500およびネットコンベア、送帯式 10 00などを用いることができる。蒸熱温度は、杜仲葉の大きさに応じて、例えば 90〜1 20°C、好ましくは 95〜110°C、より好ましくは 100〜110°Cの範囲で適宜選択されう る。また蒸熱時間も、 10〜240秒間、好ましくは 20〜180秒間、より好ましくは 20〜1 20秒間の範囲で適宜選択されうる。また、使用する蒸気量は、例えば 200〜70LZ 分、好ましくは 170〜： L00LZ分の範囲で適宜選択されうる。蒸し葉の処理量は、生 葉の水分率に応じて、例えば 3〜： LOkgZ分、好ましくは 4〜8kgZ分、より好ましくは 5〜7kgZ分の範囲で適宜選択されうる。この蒸熱工程は、杜仲葉を褐色に変色さ せる酵素が失活することにより杜仲葉の緑色が保たれやすくなる；および、杜仲葉が 柔らかくなることで、蒸熱工程の後の杜仲葉を攪拌および Zまたは揉圧しながら乾燥 する工程の実施が容易になる、などの効果をもたらす。 [0025] The steaming step of the cocoon leaves in the present invention can be carried out by a method usually performed in the technical field using a commercially available steamer or an autoclave. For example, it is possible to steam heat-treat cocoon leaves by spreading them on a net conveyor and passing them through a treatment chamber filled with non-pressure steam supplied from a boiler. For example, Kashimura Tekko Co., Ltd., leaf feeder, ground type 1500, net conveyor, trolley type 100, etc. can be used. The steaming temperature may be appropriately selected within the range of, for example, 90 to 120 ° C., preferably 95 to 110 ° C., more preferably 100 to 110 ° C., depending on the size of Tochu Nakaba. The steaming time can also be appropriately selected within the range of 10 to 240 seconds, preferably 20 to 180 seconds, more preferably 20 to 120 seconds. The amount of steam used can be appropriately selected within the range of, for example, 200 to 70 LZ, preferably 170 to L00LZ. The treatment amount of the steamed leaves can be appropriately selected in the range of, for example, 3 to: LO kgZ, preferably 4 to 8 kgZ, more preferably 5 to 7 kgZ, depending on the moisture content of the fresh leaves. This steaming process makes it easier to maintain the green color of the Nakanaka leaf by inactivating the enzyme that changes the color of the Nakanaka leaf to brown; The softening has the effect of facilitating the process of drying the steamed Nakanaka leaves after the steaming process with stirring and Z or pressure.
[0026] 蒸熱処理された杜仲葉は、そのまま次の工程に使用することもできる力 冷却後に 次の工程で使用することもできる。ここでの冷却は、送風などにより荒熱を取り除くこと により行われうる。  [0026] The steamed and heat-treated Tochu Nakaba can be used in the next step as it is. It can also be used in the next step after cooling. The cooling here can be performed by removing rough heat by blowing air or the like.
[0027] 本発明にお 、て、杜仲葉を攪拌および Zまたは揉圧しながら乾燥する工程は、例 えば、市販されている連続式葉打機、回転式葉打機、回分型葉打機または粗揉機な どを用いて、当該技術分野で通常行われている方法により実施することができる。例 えば市販の葉打機としては、カワサキ機工株式会社製、葉打機（回分型) 60K、 90Κ 、 120Kおよび 180Kなどを用いることができる。例えば、本工程における揉圧は、適 当な弾性を有する板パネで支えられた「より手」が葉打機内で回転し、処理胴の壁面 に杜仲葉を押さえつけることにより行われる。また、本工程における攪拌は、葉打機 に取り付けられた「葉ざらい」が回転することにより行われる。本工程おける揉圧は、 緩やかに行うのが好ましぐ例えば葉ざらいの回転のみで行ってもよい。乾燥方法は 特に限定されないが、好ましくは杜仲葉に熱風を送ることにより行われる。ここで熱風 の温度は、特に限定されないが、例えば 70〜120°C、好ましくは 75〜110°Cの範囲 から適宜選択されうる。また本工程に要する時間は、 10〜60分間、好ましくは 15〜5 0分間、より好ましくは 20〜40分間の範囲で適宜選択されうる。例えば本工程は、 10 0°Cで 20〜30分間行うことができる。本工程により、杜仲葉各部分の水分を均一に 保ちつつ効率よく杜仲葉が乾燥される。また本工程における攪拌および Zまたは揉 圧により、杜仲葉に含まれる糖質、脂質などの粘性物質が杜仲葉から分離され、その 結果得られる杜仲茶の「えぐ味」および「青臭さ」が低減される。本工程は、好ましくは 、水分の杜仲葉表面力 の蒸散速度と杜仲葉内部での水分拡散速度が平衡となる ように行われる。また、得られる杜仲茶の「えぐ味」および「青臭さ」を低減するために 、葉打ち機内の密封性が高いほうが好ましい。このような条件を保ちながら行うことに より、杜仲葉表面がしめった状態を保ち、杜仲葉の表面温度を一定範囲内に保つこ とができ、杜仲生葉の変色を抑制しつつ乾燥させることが可能となる。ここで、本工程 における乾燥時の杜仲茶葉表面温度は、例えば 30〜60°C、好ましくは 40〜50°Cで ある。本工程を経て得られる杜仲葉の水分量は、例えば乾量基準で 30〜45%、好 ましくは 30〜40%、より好ましくは 30〜35%である。 [0027] In the present invention, the step of drying the bamboo leaf while stirring and Z or under pressure includes, for example, a commercially available continuous leaf cutter, rotary leaf cutter, and batch type leaf cutter. Alternatively, it can be carried out by a method commonly used in the technical field using a roughing machine or the like. For example, as a commercially available leaf cutter, leaf cutters (batch type) 60K, 90 機, 120K, and 180K manufactured by Kawasaki Kiko Co., Ltd. can be used. For example, the crushing pressure in this process is performed by a “more hand” supported by a panel panel having an appropriate elasticity rotating in the leaf cutter and pressing the corrugated leaf against the wall of the processing cylinder. In addition, the stirring in this step is performed by rotating the “leaf crust” attached to the leaf cutter. The rolling pressure in this step is preferably performed gently, for example, by only rotating the leaves. The drying method is not particularly limited, but is preferably performed by sending hot air to Tochu Nakaba. Here, the temperature of the hot air is not particularly limited, but may be appropriately selected from the range of, for example, 70 to 120 ° C, preferably 75 to 110 ° C. The time required for this step can be appropriately selected within the range of 10 to 60 minutes, preferably 15 to 50 minutes, more preferably 20 to 40 minutes. For example, this step can be performed at 100 ° C. for 20-30 minutes. By this step, the Nakanaka leaves are efficiently dried while keeping the moisture in each part of the Nakanaka leaves uniform. In addition, due to the stirring and Z or pressure in this process, sugars, lipids, and other viscous substances contained in Tochu Naka are separated from Tochu Naka, and the resulting “Egu taste” and “Blue odor” of Tochu tea are reduced. Is done. This step is preferably performed so that the transpiration rate of the surface strength of the water of the Nakanaka leaf and the moisture diffusion rate inside the Nakanaka leaf are in equilibrium. In addition, in order to reduce the “egu taste” and “blue odor” of the resulting Tochu tea, it is preferable that the sealing performance in the leaf cutter is high. By carrying out while maintaining these conditions, the surface of the nakanaka leaf can be maintained, the surface temperature of the nakanaka leaf can be kept within a certain range, and drying can be performed while suppressing discoloration of the nakanaka leaf. It becomes possible. Here, the surface temperature of Tochu tea leaves during drying in this step is, for example, 30 to 60 ° C, preferably 40 to 50 ° C. is there. The water content of Tochu Nakaba obtained through this step is, for example, 30 to 45%, preferably 30 to 40%, more preferably 30 to 35% on a dry basis.
[0028] 本発明にお 、ては、「杜仲葉を攪拌および Zまたは揉圧しながら乾燥する工程」と「 杜仲葉を乾燥する工程」の間に、杜仲葉の水分を均一化する工程が含まれていても よい。本発明において、杜仲葉中の水分を均一化する工程は、例えば市販されてい る揉捻機、粗揉機または中揉機を用いて、当該技術分野で通常行われている方法 により実施することができる。例えば市販の揉捻機としては、株式会社寺田製作所製 、揉捻機 60Kg型などを用いることができる。本工程における杜仲葉中の水分の均一 化は、例えば、揉捻機の揉捻盤と回転胴の間に杜仲葉が挟まれ、かつ揉捻盤により 加圧されると同時に回転胴が回転することにより行われる。杜仲葉は葉肉、葉脈、葉 軸の順に乾燥するので、例えば葉肉の乾燥度が十分な場合でも葉軸にはまだ余分 な水分が残っている。従って、本工程により、杜仲葉中の水分が均一に整えられ、部 分乾燥による粉体ィ匕が抑制されるのと同時に、乾燥に要する時間を短縮することが できる。本工程は、必要に応じて加熱下で行うこともできる力 好ましくは加熱せずに 行われる。また本工程に要する時間は、 10〜80分間、好ましくは 20〜60分間、より 好ましくは 30〜45分間の範囲で適宜選択されうる。例えば本工程は、常温で 40分 間行うことができる。本工程中に揉捻盤による加圧がなされる力 工程開始時の無加 圧時間は、 0〜10分間、好ましくは 2〜8分間、より好ましくは 4〜5分間の範囲で適 宜選択されうる。本工程を経て得られる杜仲葉の水分量は、例えば乾量基準で 25〜 40%、好ましくは 25〜35%である。  [0028] The present invention includes a step of homogenizing the moisture of the chunaka leaf between "the step of drying the chunaka leaf with stirring and Z or pressure" and "the step of drying the chunaka leaf". It may be. In the present invention, the step of homogenizing the water in the bamboo leaf can be carried out by a method usually performed in the technical field using, for example, a commercially available twister, coarser, or intermediater. For example, as a commercially available twister, Terada Seisakusho Co., Ltd., a twister 60 kg type can be used. The homogenization of the water in the cocoon leaf in this process is performed, for example, by sandwiching the cocoon leaf between the turret and the rotating drum of the turret and pressurizing with the rotator while rotating the rotator. Is called. Since the Tochu leaves are dried in the order of mesophyll, vein, and leaf shaft, for example, even if the dryness of the leaf meat is sufficient, excess moisture still remains on the leaf shaft. Therefore, according to this step, the moisture in the bamboo leaf is uniformly prepared, and the powdery wrinkles due to partial drying are suppressed, and at the same time, the time required for drying can be shortened. This step can be performed with heating as required, preferably without heating. The time required for this step can be appropriately selected within the range of 10 to 80 minutes, preferably 20 to 60 minutes, more preferably 30 to 45 minutes. For example, this process can be performed at room temperature for 40 minutes. The force applied by the scissors during this process The no-pressing time at the start of the process can be appropriately selected in the range of 0 to 10 minutes, preferably 2 to 8 minutes, more preferably 4 to 5 minutes. . The moisture content of the chunaka leaf obtained through this step is, for example, 25 to 40%, preferably 25 to 35% on a dry basis.
[0029] 本発明に「杜仲葉中の水分を均一化する工程」が含まれる場合、杜仲葉を乾燥す る工程の前に、追加の「杜仲葉を攪拌および Zまたは揉圧しながら乾燥する工程」お よび「杜仲葉中の水分を均一化する工程」、または「杜仲葉を攪拌および Zまたは揉 圧しながら乾燥する工程」が含まれて 、てもよ 、。これらの工程を繰り返すことにより、 不要な脂質などがさらに取り除かれた杜仲茶葉を得ることができる。  [0029] In the case where the present invention includes "a step of homogenizing moisture in the chunaka leaf", an additional "step of drying the chunaka leaf with stirring and Z or under pressure before the step of drying the chunaka leaf. And “the step of homogenizing the water in the Tochu leaf”, or “the step of drying the Tochu leaf with stirring and Z or pressure” may be included. By repeating these steps, it is possible to obtain Tochu tea leaves from which unnecessary lipids have been further removed.
[0030] 「杜仲葉を攪拌および Zまたは揉圧しながら乾燥する工程」を追加する場合、当該 工程は上述の方法および条件で行うことができ、熱風の温度は、特に限定されない 力 例えば 50〜110°C、好ましくは 55〜105°Cの範囲力も適宜選択されうる。また本 工程に要する時間は、 5〜45分間、好ましくは 10〜40分間、より好ましくは 10〜35 分間の範囲で適宜選択されうる。例えば 70°Cで 20〜30分間行うことができる。 [0030] In the case of adding "a step of drying konnakaba with stirring and Z or under pressure", the step can be performed by the above-described method and conditions, and the temperature of the hot air is not particularly limited. For example, 50 to 110 A range force of ° C, preferably 55-105 ° C can also be selected as appropriate. Also book The time required for the step can be appropriately selected within the range of 5 to 45 minutes, preferably 10 to 40 minutes, more preferably 10 to 35 minutes. For example, it can be performed at 70 ° C for 20 to 30 minutes.
[0031] 「杜仲葉中の水分を均一化する工程」を追加する場合、当該工程は上述の方法お よび条件で行うことができ、また本工程に要する時間は、 10〜80分間、好ましくは 20 〜60分間、より好ましくは 30〜45分間の範囲で適宜選択されうる。例えば常温で 40 分間行うことができる。 [0031] In the case of adding "a step of homogenizing moisture in Tochu-nakaba", the step can be performed by the above-mentioned method and conditions, and the time required for this step is 10 to 80 minutes, preferably It can be appropriately selected within a range of 20 to 60 minutes, more preferably 30 to 45 minutes. For example, it can be performed at room temperature for 40 minutes.
[0032] 本発明の杜仲葉を乾燥する工程は、例えば、市販されている乾燥機を用いて当該 技術分野で通常行われている方法により実施することができる。本工程における乾燥 方法は、特に限定はされないが、例えば、搬送コンベア上の杜仲葉を、熱風発生器 による熱風で満たされた高温の乾燥室内を移動させることにより行うことができる。例 えば、株式会社寺田製作所製、乾燥機 ND120型により行われうる。ここで熱風の温 度は、特に限定されないが、例えば 70〜100°C、好ましくは 85〜95°Cの範囲力も適 宜選択されうる。また本工程に要する時間は、 5〜80分間、好ましくは 10〜70分間、 より好ましくは 20〜80分間の範囲で適宜選択されうる。本工程を経て得られる杜仲 葉の水分量は、例えば水分率 5%以下、好ましくは水分率 3%以下、より好ましくは水 分率 2%以下である。  [0032] The step of drying the Tochu leaf of the present invention can be carried out, for example, by a method commonly performed in the technical field using a commercially available dryer. Although the drying method in this process is not specifically limited, For example, it can carry out by moving the Tochu Naka leaf on a conveyance conveyor in the high temperature drying chamber filled with the hot air by a hot air generator. For example, it can be performed by a dryer ND120 manufactured by Terada Manufacturing Co., Ltd. Here, the temperature of the hot air is not particularly limited, but a range force of, for example, 70 to 100 ° C., preferably 85 to 95 ° C. may be appropriately selected. The time required for this step can be appropriately selected within the range of 5 to 80 minutes, preferably 10 to 70 minutes, more preferably 20 to 80 minutes. The water content of the bamboo leaf obtained through this step is, for example, a water content of 5% or less, preferably a water content of 3% or less, more preferably a water content of 2% or less.
[0033] 本発明においては、杜仲葉に対して遠赤外線を照射することにより杜仲葉を乾燥 する工程を含んでいてもよい。当該工程は、特に限定はされないが、例えば市販され ている遠赤外線ヒーターを用いて行うことができる。例えば、山益製作所株式会社製 、 VR型により行われうる。ここで照射される遠赤外線の波長は、例えば 1〜： LOOO /z m、好ましくは 2. 5〜50 μ m、より好ましくは 3〜30 μ mの範囲から適宜選択される。 乾燥中の照射設定温度は、例えば 100〜400°C、好ましくは 150〜350°C、より好ま しくは 200〜300°Cの範囲の範囲より適宜選択されうる。また本工程に要する時間は 、赤外線ランプ通過速度として 30〜60秒間、好ましくは 40〜55秒間、より好ましくは 45〜50秒間の範囲で適宜選択されうる。本工程を経て得られる乾燥杜仲葉の水分 量は、例えば水分率 5%以下、好ましくは水分率 3%以下、より好ましくは水分率 2% 以下である。  [0033] The present invention may include a step of drying the chunaka leaf by irradiating the chunaka leaf with far infrared rays. Although this process is not specifically limited, For example, it can be performed using the commercially available far-infrared heater. For example, it can be performed by a VR type manufactured by Yamamasu Seisakusho Co., Ltd. The wavelength of the far infrared rays irradiated here is appropriately selected from the range of, for example, 1 to: LOOO / z m, preferably 2.5 to 50 μm, more preferably 3 to 30 μm. The irradiation set temperature during drying can be appropriately selected from the range of, for example, 100 to 400 ° C, preferably 150 to 350 ° C, more preferably 200 to 300 ° C. The time required for this step can be appropriately selected within the range of 30 to 60 seconds, preferably 40 to 55 seconds, and more preferably 45 to 50 seconds as the infrared lamp passing speed. The moisture content of the dried bamboo leaf obtained through this step is, for example, a moisture content of 5% or less, preferably a moisture content of 3% or less, more preferably a moisture content of 2% or less.
[0034] 本明細書において杜仲葉水抽出物は、乾燥杜仲葉 lkgに対して、例えば 5〜50k g、好ましくは 10〜30kg、より好ましくは 15〜20kg力 適宜選択される量の水が用 いることができる。抽出温度は、例えば 85〜98°C、好ましくは 90〜95°Cの範囲から 適宜選択されうる。抽出時間は、特に限定はされないが、例えば 10〜120分、好まし くは 20〜90分、より好ましくは 30〜60分力 適宜選択されうる。 [0034] In the present specification, the Tochu Nakaba water extract is, for example, 5 to 50 k with respect to 1 kg of dried Tochu Nakaba. g, preferably 10-30 kg, more preferably 15-20 kg force An appropriately selected amount of water can be used. The extraction temperature can be appropriately selected from the range of, for example, 85 to 98 ° C, preferably 90 to 95 ° C. The extraction time is not particularly limited, but can be appropriately selected, for example, 10 to 120 minutes, preferably 20 to 90 minutes, more preferably 30 to 60 minutes.
[0035] 抽出液の濾過は、例えば、ざる、または 30〜200メッシュのフィルターなどを用いて 行われうる。濾液は濃縮を行う前に一定時間静置してもよい。静置することにより発生 する沈殿物を除去することにより、不要物を取り除くことができる。静置する時間は、 特に限定はされないが、例えば 1〜24時間、好ましくは 6〜20時間、より好ましくは 8 〜18時間から適宜選択されうる。静置する際の温度は、特に限定はされないが、例 えば 0〜35°C、好ましくは 0〜16°C、より好ましくは 2〜8°C力も適宜選択されうる。  [0035] Filtration of the extract may be performed using, for example, a filter or a 30-200 mesh filter. The filtrate may be allowed to stand for a certain period before concentration. Unnecessary substances can be removed by removing precipitates generated by standing. The standing time is not particularly limited, but may be appropriately selected from, for example, 1 to 24 hours, preferably 6 to 20 hours, and more preferably 8 to 18 hours. The temperature at the time of standing is not particularly limited. For example, a force of 0 to 35 ° C, preferably 0 to 16 ° C, more preferably 2 to 8 ° C can be appropriately selected.
[0036] こうして得られる杜仲葉抽出液をそのまま使用することもできるが、遠心分離に付し て生じる沈殿を除去した後に得られる上澄みを次の工程に使用することもできる。さ らに、当該上澄み液を濃縮して使用することもできる。  [0036] The soot extract obtained in this way can be used as it is, but the supernatant obtained after removing the precipitate generated by centrifugation can also be used in the next step. Further, the supernatant can be used after being concentrated.
[0037] 本発明で使用される「逆相の合成吸着剤」は、天然成分の分離などに通常使用さ れるものであれば特に限定されないが、ポリスチレンゲル（ポリスチレン 'ジビュルベン ゼン共重合体など）、例えば、 MCIGEL CHP10M/CHP2MGM, SEPABEA  [0037] The "reverse-phase synthetic adsorbent" used in the present invention is not particularly limited as long as it is usually used for separation of natural components, etc., but polystyrene gel (polystyrene 'dibulene benzene copolymer, etc.) For example, MCIGEL CHP10M / CHP2MGM, SEPABEA
P2MGY/SP207/SP825/SP850/SP70/SP700, Diaion HP20/HP2 1ZHP2MGなどが挙げられ、工業的スケールにおける製造の観点から好ましくは D iaion HP20が使用される。当該吸着剤は、例えばカラムの担体として使用すること ができる。使用される吸着剤の量は、杜仲葉抽出液 110Lに対して、例えば 10000 〜 18000g、好まし <は 15000〜 18000gの範囲力も選択されうる。 P2MGY / SP207 / SP825 / SP850 / SP70 / SP700, Diaion HP20 / HP2 1ZHP2MG and the like, and Diaion HP20 is preferably used from the viewpoint of production on an industrial scale. The adsorbent can be used as a column carrier, for example. The amount of adsorbent used can be selected, for example, in the range of 10000 to 18000 g, preferably <15000 to 18000 g, for 110 L of Tochu leaf extract.
[0038] 本発明の 1つの態様において、逆相の吸着剤を充填させたカラムに杜仲葉抽出液 を注入し、その後 1定量の水を溶出液として使用して溶出する成分を本発明の分画 物として得ることができる。ここで、溶出液として使用する水の量は特には限定されな いが、例えば、吸着剤 17200g対して、 70〜： L 10Lの範囲力 選択される量で使用 することができる。 [0038] In one embodiment of the present invention, a Tochu leaf extract is injected into a column packed with a reverse-phase adsorbent, and then a component that is eluted using a fixed amount of water as an eluent is separated into the components of the present invention. It can be obtained as a picture. Here, the amount of water to be used as the eluent is not particularly limited. For example, a range force of 70 to L 10 L can be used for 17200 g of the adsorbent.
[0039] 溶媒を留去した後の固形成分中のァスペル口サイドの含有量は、例えば、 HPLC などを使用した分析方法（吸光度の測定波長： 215nm)により決定されうる。ァゥクビ ン（吸光度の測定波長： 215nm)、クロロゲン酸（吸光度の測定波長： 215nm)およ びゲ-ポシド酸（吸光度の測定波長： 215nm)についても同様の方法により含有量を 柳』定することができる。 [0039] The content of the asperal mouth side in the solid component after the solvent is distilled off is, for example, HPLC And the like (the measurement wavelength of absorbance: 215 nm). The content of aucubin (absorbance measurement wavelength: 215 nm), chlorogenic acid (absorbance measurement wavelength: 215 nm) and geposide acid (absorbance measurement wavelength: 215 nm) should be determined in the same manner. Can do.
[0040] 本発明の分画物は粉体として得ることができる。当該粉体に含まれるァスペルロサ イドの含有量は、溶媒を留去した後の固形成分総量に対し 1重量％以下である。一 例として 0. 01重量％以下が挙げられる。  [0040] The fraction of the present invention can be obtained as a powder. The content of asperuloside contained in the powder is 1% by weight or less based on the total amount of solid components after the solvent is distilled off. An example is 0.01% by weight or less.
[0041] 当該粉体に含まれるァゥクビンの含有量は、溶媒を留去した後の固形成分総量に 対し 15. 0〜18. 0重量0 /0であり、好ましくは 16. 0〜18. 0重量0 /0、より好ましくは 16 . 4〜17. 4重量％である。一例として 16. 9重量％が挙げられる。 [0041] The content of Aukubin contained in the powder is 15.0 to 18 against the solid component amount of After evaporation of the solvent. 0 weight 0/0, preferably 16.0 to 18.0 weight 0/0, more preferably 16.4 to 17.4 wt%. An example is 16.9% by weight.
[0042] 当該粉体に含まれるクロロゲン酸の含有量は、溶媒を留去した後の固形成分総量 に対し 5. 61〜6. 85重量0 /0であり、好ましくは 5. 92〜6. 54重量0 /0、より好ましくは[0042] The content of chlorogenic acids contained in the powder is from 5.61 to 6.85 wt 0/0 relative to the solid component amount of After evaporation of the solvent, preferably from 5.92 to 6. 54 weight 0/0, more preferably
6. 04-6. 42重量％である。一例として 6. 2重量％が挙げられる。 6. 04-6. 42% by weight. An example is 6.2% by weight.
[0043] 当該粉体に含まれるゲ-ポシド酸の含有量は、溶媒を留去した後の固形成分総量 に対し 6. 50〜7. 94重量0 /0であり、好ましくは 6. 86〜7. 58重量0 /0、より好ましくは[0043] gate included in the powder -. The content of Poshido acid is from 6.50 to 7 94 weight 0/0 relative to the solid component amount of After evaporation of the solvent, preferably 6. 86 to 7.58 weight 0/0, and more preferably
7. 00-7. 44重量％である。一例として 7. 2重量％が挙げられる。 7. 00-7. 44% by weight. An example is 7.2% by weight.
[0044] 当該粉体に含まれるァゥクビン、クロロゲン酸、ゲ-ポシド酸の含有量の総量は、溶 媒を留去した後の固形成分総量に対し 27. 11-32. 79重量％であり、好ましくは 2 [0044] The total content of alkvin, chlorogenic acid, and geposide acid contained in the powder is 27.11-32.79 wt% with respect to the total amount of solid components after the solvent is distilled off, Preferably 2
8. 78〜32. 12重量0 /0、より好ましくは 29. 44〜31. 26重量0 /0である。一例として 3 0. 3重量％が挙げられる。 8.78 to 32.12 wt 0/0, more preferably from 29.44 to 31.26 wt 0/0. An example is 30.3 wt%.
[0045] また、本発明の分画物はタール状物として得ることができる。当該タール状物に含 まれるァスペル口サイドの含有量は、溶媒を留去した後の固形成分総量に対し 1重量 %以下であり、一例として 0. 01重量％以下が挙げられる。  [0045] The fraction of the present invention can be obtained as a tar-like product. The content of the asper mouth side contained in the tar-like product is 1% by weight or less with respect to the total amount of solid components after the solvent is distilled off, and an example is 0.01% by weight or less.
[0046] 当該タール状物に含まれるァゥクビンの含有量は、溶媒を留去した後の固形成分 総量に対し 13. 5-16. 5重量％であり、好ましくは 14. 3-15. 8重量0 /0、より好まし くは 14. 6-15. 4重量％である。一例として 15. 0重量％が挙げられる。 [0046] The content of alkvin contained in the tar-like product is 13.5-16. 5% by weight, preferably 14.3-15.8% by weight based on the total amount of solid components after the solvent is distilled off. 0/0, more rather preferably is 14. 6-15. 4 wt%. An example is 15.0% by weight.
[0047] 当該タール状物に含まれるクロロゲン酸の含有量は、溶媒を留去した後の固形成 分総量に対し 5. 14〜6. 28重量0 /0であり、好ましくは 5. 42〜6. 00重量0 /0、より好 ましくは 5. 54-5. 88重量％である。一例として 5. 7重量％が挙げられる。 [0047] The content of chlorogenic acids contained in the tar-like product is 14 to 6.28 wt 0/0 5. to solid formation amount total after evaporation of the solvent, preferably 5. 42 to 6.00 weight 0/0, more virtuous It is preferably 5.54-5.88% by weight. An example is 5.7% by weight.
[0048] 当該タール状物に含まれるゲ-ポシド酸の含有量は、溶媒を留去した後の固形成 分総量に対し 5. 90-7. 22重量0 /0であり、好ましくは 6. 23-6. 89重量0 /0、より好 ましくは 6. 36-6. 76重量％である。一例として 6. 6重量％が挙げられる。 [0048] gate contained in the tar-like product -. The content of Poshido acid is 5. 90-7 22 weight 0/0 to solid formation amount total after evaporation of the solvent, preferably 6. 23-6. 89 weight 0/0, which is the more favorable Mashiku 6. 36-6. 76 wt%. An example is 6.6% by weight.
[0049] 当該タール状物に含まれるァゥクビン、クロロゲン酸、ゲ-ポシド酸の含有量の総量 は、溶媒を留去した後の固形成分総量に対し 24. 54-30. 0重量％であり、好ましく は 25. 95〜28. 69重量0 /0、より好ましくは 26. 50〜28. 04重量0 /0である。一例とし て 27. 3重量％が挙げられる。 [0049] The total amount of alkvin, chlorogenic acid, and geoposide acid contained in the tar-like product is 24.54-300.0% by weight based on the total amount of solid components after the solvent is distilled off, preferably from 25.95 to 28.69 wt 0/0, more preferably from 26.50 to 28.04 wt 0/0. An example is 27.3% by weight.
[0050] 水溶液として得られる分画物は、好ましくは、濃縮して使用される。濃縮率は特に限 定されないが、例えば容積比で 60%以下であってよぐ好ましくは 30%以下であり、 さらに好ましくは乾固して得られる固体物として使用される。また当該濃縮は常圧下 または減圧下のいずれにおいても行われうる力 好ましくは減圧下で行われる。濃縮 方法は、通常使用される方法であれば特に限定されず、例えばロータリーエバポレ 一ターなどを使用して濃縮することができる。  [0050] The fraction obtained as an aqueous solution is preferably used after being concentrated. The concentration rate is not particularly limited, but is, for example, 60% or less in volume ratio, preferably 30% or less, and more preferably used as a solid product obtained by drying. In addition, the concentration can be performed under normal pressure or reduced pressure, preferably under reduced pressure. The concentration method is not particularly limited as long as it is a commonly used method, and for example, it can be concentrated using a rotary evaporator.
[0051] 本発明の分画物は、血圧降下またはリパーゼ阻害などの薬効を有する医薬組成物 の有効成分として使用することができる。当該医薬組成物は、種々の剤形、例えば、 経口投与のためには、錠剤、カプセル剤、散剤、顆粒剤、丸剤、液剤、乳剤、懸濁液 、溶液剤、酒精剤、シロップ剤、エキス剤、エリキシル剤などとすることができ、例えば 、局所投与のためにはクリーム、ゼリー、ゲル、ペースト、軟膏などとすることができる 力 これらには限定されない。当該医薬組成物は、一般に用いられる各種成分を含 みうるものであり、例えば、 1種もしくはそれ以上の薬学的に許容され得る賦形剤、崩 壊剤、希釈剤、滑沢剤、着香剤、着色剤、甘味剤、矯味剤、懸濁化剤、湿潤剤、乳 ィ匕剤、分散剤、補助剤、防腐剤、緩衝剤、結合剤、安定剤、コーティング剤等を含み うる。また本発明の医薬組成物は、持続性または徐放性剤形であってもよい。  [0051] The fraction of the present invention can be used as an active ingredient of a pharmaceutical composition having a medicinal effect such as blood pressure lowering or lipase inhibition. The pharmaceutical composition can be used in various dosage forms such as tablets, capsules, powders, granules, pills, solutions, emulsions, suspensions, solutions, spirits, syrups, for oral administration. Extracts, elixirs, and the like can be used. For example, for topical administration, creams, jellies, gels, pastes, ointments, and the like can be used. The pharmaceutical composition may contain various commonly used ingredients, for example, one or more pharmaceutically acceptable excipients, disintegrants, diluents, lubricants, flavors. Agents, coloring agents, sweeteners, flavoring agents, suspending agents, wetting agents, emulsifying agents, dispersing agents, adjuvants, preservatives, buffering agents, binders, stabilizers, coating agents and the like can be included. The pharmaceutical composition of the present invention may be in a sustained or sustained release dosage form.
[0052] 本発明の分画物は、例えば錠剤の成分として使用することができる。当該錠剤は、 医薬製剤の分野において通常使用される製造方法により製造され、例えば、結晶セ ルロース、二酸化ケイ素、ショ糖脂肪酸エステル、酵母細胞壁、グリセリンなどの添カロ 剤を使用することができる。また、賦形剤 (例えば、麦芽糖、デキストリン、サイクロデキ ストリン、寒天、トウモロコシたん白、リン酸三カルシウム、カルボキシメチルセルロース カルシウム、植物油脂、グリセリン脂肪酸エステル、アラビアガム）を使用することもで きるが、本発明の分画物は、好ましくは賦形剤を使用することなく製造することができ 、杜仲葉抽出物の含有量の高い錠剤を製造することができる。得られた錠剤は、表 面に粘性が少なぐ包材封入後も錠剤間の溶着が生じにくい。また長期間の高温下（[0052] The fraction of the present invention can be used, for example, as a component of a tablet. The tablet is produced by a production method usually used in the field of pharmaceutical preparations, and for example, supplemental carotenants such as crystalline cellulose, silicon dioxide, sucrose fatty acid ester, yeast cell wall, glycerin and the like can be used. In addition, excipients (for example, maltose, dextrin, cyclodex (String, agar, corn protein, tricalcium phosphate, carboxymethylcellulose calcium, vegetable oil, glycerin fatty acid ester, gum arabic), but the fraction of the present invention preferably contains an excipient. It can be produced without using it, and a tablet with a high content of Tochu Nakaba extract can be produced. The obtained tablets are less likely to weld between the tablets even after enclosing the packaging material with low viscosity on the surface. Also for long periods of high temperatures (
35°C)、例えば 2週間経過しても粘着は認められない。 35 ° C) For example, no adhesion is observed after 2 weeks.
[0053] 例えば上記錠剤の成分として使用することができる。当該錠剤は、医薬製剤の分野 において通常使用される製造方法により製造され、例えば、結晶セルロース、二酸ィ匕 ケィ素、ショ糖脂肪酸エステル、酵母細胞壁、グリセリンなどの添加剤を使用すること ができる。 [0053] For example, it can be used as a component of the tablet. The tablet is produced by a production method usually used in the field of pharmaceutical preparations. For example, additives such as crystalline cellulose, diacid carbonate, sucrose fatty acid ester, yeast cell wall, and glycerin can be used. .
[0054] 例えば本発明の分画物は、粉体のまま使用することができる。当該粉体は、粒状で も粘性が少なく吸湿性も低いため、包材封入も容易である。また、包材封入後も粉体 の溶着 ·固化が生じにくい。長期間の高温下 (35°C)、例えば 2週間経過しても粒状を 保つ。  [0054] For example, the fraction of the present invention can be used as a powder. Since the powder is granular, it has low viscosity and low hygroscopicity, so that it can be easily encapsulated. In addition, the powder is less likely to be welded or solidified after the packaging material is enclosed. Maintains granularity even at high temperatures (35 ° C) for a long time, for example, 2 weeks.
[0055] 本発明の抗肥満剤の投与量は、患者の体型、年齢、体調、疾患の度合い、発症後 の経過時間等により、適宜選択することができ、本発明の医薬組成物は、治療有効 量および Zまたは予防有効量の抗肥満剤を含むことができる。例えば本発明の杜仲 葉粉砕物またはその水抽出物として、一般に 10〜50000mg/日/成人、好ましく は 100〜5000mgZ日 Z成人の用量で使用される。当該医薬組成物の投与は、単 回投与または複数回投与であってもよぐ例えば他の抗肥満剤などの他の薬剤と組 み合わせて使用することもできる。  [0055] The dose of the anti-obesity agent of the present invention can be appropriately selected depending on the patient's body shape, age, physical condition, degree of disease, elapsed time after onset, etc. The pharmaceutical composition of the present invention is a therapeutic agent. An effective amount and Z or a prophylactically effective amount of an anti-obesity agent can be included. For example, it is generally used in the dose of 10 to 50000 mg / day / adult, preferably 100 to 5000 mg Z day Z adult, as the pulverized mash of the present invention or its water extract. Administration of the pharmaceutical composition can be single dose or multiple doses and can be used in combination with other drugs such as other anti-obesity agents.
[0056] 本発明に係る食品は、液体飲料および固形の食品を含む。当該食品は、医薬部外 品、他の飲食物などの成分、食品添加物などとして使用することができる。また本明 細書における経口摂取用組成物は、そのまま機能性食品として使用できるほか、医 薬品、医薬部外品、飲食物等の成分、食品添加物などとして使用することができる。 当該使用により、本発明の肥満改善効果および動脈硬化予防剤を有する当該食品 または経口摂取用組成物の日常的および継続的な摂取が可能となり、肥満の効果 的な改善、および肥満に関連する疾患 (例えば、高血圧症、高脂血症、糖尿病、）の 効果的な予防が可能となる。 [0056] The food according to the present invention includes a liquid beverage and a solid food. The food can be used as quasi-drugs, other food and drink ingredients, food additives, and the like. In addition, the composition for ingestion in this specification can be used as a functional food as it is, and can be used as a component of a pharmaceutical, a quasi-drug, a food and drink, and a food additive. The use enables daily and continuous ingestion of the food or composition for oral consumption having the obesity-improving effect and arteriosclerosis-preventing agent of the present invention, effectively improving obesity, and obesity-related diseases. (Eg hypertension, hyperlipidemia, diabetes) Effective prevention is possible.
[0057] 本発明の抗肥満剤を含む食品または飲料の例としては、降圧効果、リパーゼ阻害 効果もしくは肥満抑制効果などを有する機能性食品、健康食品、健康補助食品、栄 養補助食品 (栄養ドリンク等)、保険機能食品、特定保険用食品、栄養機能食品、一 般食品（ジュース、菓子、加工食品等)などが含まれる。本明細書における食品また は飲料は、任意の添加物として、鉄およびカルシウムなどの無機成分、種々のビタミ ン類、オリゴ糖およびキトサンなどの食物繊維、大豆抽出物などのタンパク質、レシチ ンなどの脂質、ショ糖および乳糖などの糖類を含むことができる。 [0057] Examples of foods or beverages containing the anti-obesity agent of the present invention include functional foods, health foods, health supplements, nutritional supplements (nutrient drinks) having a hypotensive effect, a lipase inhibitory effect or an obesity inhibitory effect, etc. Etc.), functional insurance food, food for specified insurance, functional nutrition food, general food (juice, confectionery, processed food, etc.). The food or beverage in this specification includes, as optional additives, inorganic components such as iron and calcium, various vitamins, dietary fibers such as oligosaccharides and chitosan, proteins such as soy extract, and lecithin. Sugars such as lipids, sucrose and lactose can be included.
実施例  Example
[0058] 以下、本発明の好適な実施例についてさらに詳細に説明する力 本発明はこれら の実施例に限定されるものではない。  [0058] In the following, the power to explain preferred embodiments of the present invention in more detail The present invention is not limited to these embodiments.
[0059] [実施例 1] 合成吸着剤による乾燥杜仲葉水抽出物分画物の調製 [0059] [Example 1] Preparation of a dried persimmon Nakaba water extract fraction using a synthetic adsorbent
乾燥杜仲葉は、特開 2005— 1287469号公報の実施例 4の記載に基づいて製造 される乾燥杜仲葉を使用した。  As dried dried Nakanaka, dried dried Nakanaka manufactured according to the description in Example 4 of JP-A-2005-1287469 was used.
[0060] 乾燥杜仲葉（10kg)にイオン交換水 (70L)を加え、 65°Cで 7時間加熱した。加熱 後、一晩放置した。ざる (テトロン 3号)を使用して杜仲葉残渣を除去した後に、連続 遠心分離を行!ヽ (遠心加速度： 9340xg； S型超高速遠心分離機 U— 6— H：関西遠 心分離機製作所社製)、上澄み液 (約 55L)を得た。同様の手法でもう 1バッチ行い、 杜仲葉抽出物を合わせて約 110Lの溶液として得た。 [0060] Ion-exchanged water (70 L) was added to dry potato Nakaba (10 kg) and heated at 65 ° C for 7 hours. After heating, it was left overnight. Use Zaru (Tetron No. 3) to remove the residue from the Nakanaka leaf, and then perform continuous centrifugation! ヽ (centrifugal acceleration: 9340xg; S-type ultra-high speed centrifuge U— 6— H: Kansai Enshin Separator ), And a supernatant (about 55 L) was obtained. Another batch was performed in the same manner, and the Tochu leaf extract was combined to obtain a solution of about 110 L.
[0061] 得られた溶液を、 Diaion HP20を充填したカラム（直径 135x長さ 1200mm)に注 入した。カラム力も溶出する水溶液を回収し、その後さらに 70Lの水を使用して水に より溶出を行った。 [0061] The obtained solution was poured into a column (diameter 135x length 1200mm) packed with Diaion HP20. The aqueous solution that also eluted the column force was collected, and then further 70 L of water was used to elute with water.
[0062] 得られた水溶液を、減圧下 (0. 5kPa)、 65°Cで濃縮し、タール状物表面に生じる 粉体を都度取り除きながら乾固させ。溶液画分総量中 (4520. Og)、粉体の画分 A( 1930. Og)とタール状の画分 B (2590. Og)を得た。  [0062] The obtained aqueous solution was concentrated at 65 ° C under reduced pressure (0.5 kPa), and dried to remove the powder generated on the surface of the tar-like material each time. In the total amount of the solution fraction (4520. Og), powder fraction A (1930. Og) and tar-like fraction B (2590. Og) were obtained.
[0063] [実施例 2] 乾燥杜仲葉水抽出エキスの調製  [0063] [Example 2] Preparation of extract of dried bamboo leaf extract
乾燥杜仲葉は、特開 2005— 1287469号公報の実施例 4の記載に基づいて製造 される乾燥杜仲葉を使用した。乾燥杜仲葉 (2. 3kg)にイオン交換水（16L)を加え、 65°Cで 7時間加熱した。加熱後、一晩放置した。ざる (テトロン 3号)を使用して杜仲 葉残渣を除去した後に、連続遠心分離を行!ヽ (遠心加速度： 9340xg； S型超高速遠 心分離機 U -6-H :関西遠心分離機製作所社製)、上澄み液 (約 12L)を得た。 As dried dried Nakanaka, dried dried Nakanaka manufactured according to the description in Example 4 of JP-A-2005-1287469 was used. Add ion exchange water (16L) to dried potato Nakaba (2.3 kg) Heated at 65 ° C for 7 hours. After heating, it was left overnight. Use Zaru (Tetron No. 3) to remove Tonaka leaf residue, then perform continuous centrifugation! ヽ (centrifugal acceleration: 9340xg; S-type ultra-high speed centrifuge U-6-H: Kansai centrifuge ), And a supernatant (about 12 L) was obtained.
[0064] 得られた溶液を、減圧下 (0. 5kPa)、 65°Cで乾固するまで濃縮し、乾燥杜仲葉水 抽出エキス (652g)を固体として得た。  [0064] The obtained solution was concentrated under reduced pressure (0.5 kPa) to 65 ° C until dryness, to obtain a dried kashiwa leaf water extract (652g) as a solid.
[0065] [実施例 3] 杜仲葉水抽出物分画物の色調変化試験  [0065] [Example 3] Color tone change test of Tochu Nakaha extract extract
(1)試験方法  (1) Test method
試験溶液は、乾燥杜仲葉水抽出エキス、画分 Aおよび画分 Bをそれぞれ 1. 5g秤 量し、蒸留水を加え 500gとなるように調整し、攪拌し試験液とした。試験溶液は、透 明の密封容器に封入し 40°C下の環境下で 2週間保管した。試験液の評価は、試験 溶液での目視評価と分光高度計 (UV— 1200 :島津製作所社製）により吸光度を O D560および OD720の波長で測定を行った。  For the test solution, 1.5 g each of dried kashiwa leaf extract, fraction A and fraction B were weighed, adjusted to 500 g by adding distilled water, and stirred to obtain a test solution. The test solution was sealed in a transparent sealed container and stored at 40 ° C for 2 weeks. The test solution was evaluated by measuring the absorbance at a wavelength of OD560 and OD720 using a visual evaluation of the test solution and a spectrophotometer (UV-1200: manufactured by Shimadzu Corporation).
[0066] (2)結果 [0066] (2) Results
結果を表 1と図 2に示す。図 2に示すとおり試験液の色調は、乾燥杜仲葉水抽出ェ キスでは濁りのない薄茶褐色を呈しているのに対して、画分 Aおよび画分 Bは無色で あった。 40°Cの環境下で 2週間の試験液の色調は、乾燥杜仲葉水抽出エキスでは 濁りのない濃茶褐色を呈しているのに対して画分 Aおよび画分 Bでは僅かな着色が みられたもののほぼ無色であった。次に試験液の OD560 (色調）の吸光度は、乾燥 杜仲葉水抽出エキス、画分 Aおよび画分 Bそれぞれ試験開始時に比較して 2週間後 の数値の上昇が認められた力 乾燥杜仲葉水抽出エキスに比較して画分 Aおよび画 分 Bの数値変化は僅かであった。次に試験液の OD720 (透視度）の吸光度は、乾燥 杜仲葉水抽出エキス、画分 Aおよび画分 Bそれぞれ試験開始時に比較して 2週間後 の数値の上昇が認められた力 乾燥杜仲葉水抽出エキスに比較して画分 Aおよび画 分 Bの数値変化は僅かであった。  The results are shown in Table 1 and Figure 2. As shown in Fig. 2, the color tone of the test solution showed a light brown color with no turbidity in the dried kashiwa leaf extract extract, whereas fraction A and fraction B were colorless. The color of the test solution for 2 weeks in an environment of 40 ° C shows a dark brown color with no turbidity in the extract of dried kashiwa leaf extract, while slight coloration is seen in fraction A and fraction B It was almost colorless. Next, the absorbance of OD560 (color tone) of the test solution was increased after 2 weeks compared to the time when the test was started. Compared to the extract, the numerical changes in fraction A and fraction B were slight. Next, the absorbance of OD720 (permeability) in the test solution was increased after 2 weeks compared to the time when the test was started. Compared to the water extract, the numerical changes in fraction A and fraction B were slight.
[0067] [表 1] 表 1 試験液の経時変化 [0067] [Table 1] Table 1 Change in test solution over time
Figure imgf000016_0001
Figure imgf000016_0001
[0068] [実施例 4] 杜仲葉水抽出物分画物の粘度試験  [Example 4] Viscosity test of fraction extracted from Tochu Nakaha extract
(1)試験方法  (1) Test method
試験溶液は、実施例 3の試験溶液を供試した。試験液の粘度の評価は、デジタル 粘度計 (DV— Π+： Brook Field社製）を用いて、超低粘度用 ULアダプタ一により 計測を行った。  The test solution of Example 3 was used as the test solution. The viscosity of the test solution was measured using a digital viscometer (DV—Π +: manufactured by Brook Field) with an ultra-low viscosity UL adapter.
[0069] (2)結果 [0069] (2) Results
結果を表 2に示す。試験液の粘度は、乾燥杜仲葉水抽出エキスおよび画分 Bと比 較し、画分 Aの粘度が低力つた。  The results are shown in Table 2. The viscosity of the test solution was lower than that of the dried kashinaka leaf water extract and fraction B.
[0070] [表 2] [0070] [Table 2]
表 2 試験液の粘度
Figure imgf000016_0002
Table 2 Test solution viscosity
Figure imgf000016_0002
[0071] [実施例 5] 杜仲葉水抽出物分画物の味覚試験  [Example 5] Taste test of fraction extracted from Tochu Nakaha extract
(1)試験方法  (1) Test method
試験溶液は、実施例 3の試験溶液を供試した。試験液の味覚評価は、味覚センサ 一（味認識装置 SA402B：インセント社製)を用いて計測を行った。  The test solution of Example 3 was used as the test solution. The taste evaluation of the test solution was measured using a taste sensor 1 (taste recognition device SA402B: manufactured by Incent Corporation).
[0072] (2)結果 [0072] (2) Results
結果を表 3及び図に示す。各試験液の味覚は、それぞれの嗜好項目についてほぼ 同じ値を示しており、嗜好性が一致することが明ら力となった。  The results are shown in Table 3 and the figure. The taste of each test solution showed almost the same value for each preference item, and it became clear that the tastes matched.
[0073] [表 3] 表 3 試験液の嗜好性 [0073] [Table 3] Table 3 Test solution preference
苦味 渋味 旨味 酸味 旨味 埴味 苦味 渋味 甘味 雑味 刺激 コク 無味 0 0 0 0 0 0 0 0 0 乾燥杜仲葉水抽出エキス -14.99 0.96 6.11 4.96 -13.38 -0.23 0.47 2.65 31.14 画分 A -15.21 -0.97 5.38 4.79 -13.64 -0.29 -0.08 2.65 32.24 画分 B -15.39 -0.99 5.45 4.85 -14.17 一 0.33 -0.09 2.41 31.84  Bitterness Astringency Umami Acidity Umami Taste Bitter Taste Astringency Sweet Minority Stimulation Rich Taste No taste 0 0 0 0 0 0 0 0 0 0.97 5.38 4.79 -13.64 -0.29 -0.08 2.65 32.24 Fraction B -15.39 -0.99 5.45 4.85 -14.17 One 0.33 -0.09 2.41 31.84
[0074] [試験例 1] 杜仲葉水抽出物分画物の含有成分 [0074] [Test Example 1] Ingredients in the fraction of Tochu Nakaha extract
(1)試験方法  (1) Test method
試験溶液は、実施例 3の試験溶液を供試した。試験液の含有成分は、ァゥクビン (a ucubin)、ァスぺノレ口サイド (asperuloside)、ゲニポシド酸 (geniposidic acid)およ びクロロゲン酸 (chlorogenic acid)を標準物質として用いた。分析方法は、 HPLC (LC— 6 :島津製作所社製）を用いて、カラム： YMC— Pack ODS— A A— 312, 6. Omm X 150mm,移動層：水 Zメタノール Zリン酸 =870,130,1、カラム温 度: 40°C、検出器: UV検出器 (測定波長： 215nm)、流速： lmlZmin.として、試料 溶液を注入し、クロマトグラムを得て、各指標成分のピーク面積から含量を求めた。  The test solution of Example 3 was used as the test solution. The components contained in the test solution were a ucubin, asperuloside, geniposidic acid and chlorogenic acid as standard substances. Analytical method is HPLC (LC-6: manufactured by Shimadzu Corporation), column: YMC— Pack ODS— AA— 312, 6. Omm X 150 mm, moving bed: water Z methanol Z phosphoric acid = 870,130,1, Column temperature: 40 ° C, detector: UV detector (measurement wavelength: 215 nm), flow rate: lmlZmin., Sample solution was injected, chromatogram was obtained, and the content was determined from the peak area of each indicator component .
[0075] (2)結果  [0075] (2) Results
結果を表 4に示す。  The results are shown in Table 4.
[0076] [表 4]  [0076] [Table 4]
表 4 試験液の含有成分の定量
Figure imgf000017_0001
図面の簡単な説明 Table 4 Determination of components in test solution
Figure imgf000017_0001
Brief Description of Drawings
[図 1]杜仲葉水抽出物水溶液の味覚試験の結果の一例を示す図である。  FIG. 1 is a diagram showing an example of a taste test result of an aqueous solution of Tochu Nakaha water extract.
圆 2]実施例 3の試験液の色調を示す写真である。  圆 2] A photograph showing the color tone of the test solution of Example 3.

Claims

請求の範囲  The scope of the claims
[I] 乾燥杜仲葉から得られる杜仲葉水抽出物を逆相の合成吸着剤に吸着させ、水で 溶出する成分を得る工程を含む製造方法により得られる、杜仲葉水抽出物分画物。  [I] A foliage extract of Tochu Nakaba extract obtained by a production method comprising a step of adsorbing a Tochu Nakaba water extract obtained from dried Tochu Nakaba to a synthetic adsorbent having a reverse phase to obtain a component eluted with water.
[2] 前記水で溶出する成分として得られる分画物を濃縮し、粉体を得る工程をさらに含 む製造方法により得られる、請求項 1の杜仲葉水抽出物分画物。  [2] The Tochu Nakaba water extract fraction according to claim 1, which is obtained by a production method further comprising a step of concentrating the fraction obtained as a component eluted with water to obtain a powder.
[3] 溶媒を留去した後の固形成分として、当該固形成分の総量に対しァスペル口サイド の含有量が 1重量％以下である、請求項 1または 2に記載の杜仲葉水抽出物分画物  [3] The fraction of Tochu leaf extract according to claim 1 or 2, wherein the content of the asperal mouthside is 1% by weight or less based on the total amount of the solid component after the solvent is distilled off object
[4] 0. 3重量％水溶液としたときの 560nmにおける吸光度（OD560)が 0. 06以下で ある、請求項 1〜3のいずれか 1項に記載の杜仲葉水抽出物分画物。 [4] The foliage leaf extract fraction according to any one of claims 1 to 3, which has an absorbance (OD560) at 560 nm of 0.03 or less when a 0.3 wt% aqueous solution is prepared.
[5] 前記乾燥杜仲葉が、杜仲生葉を蒸熱する工程;杜仲葉を攪拌および Zまたは揉圧 しながら乾燥する工程;および杜仲葉に対して遠赤外線を照射することにより杜仲葉 を乾燥する工程を含む製造方法により得られる、請求項 1〜4のいずれか 1項に記載 の杜仲葉水抽出物分画物。  [5] The step of drying the dried nakanaka leaves the dried nakanaka leaves; the drying of the nakanaka leaves with stirring and Z or pressure; and the step of drying the nakanaka leaves by irradiating far-infrared rays with the infrared rays. The Nakanaka leaf water extract fraction of any one of Claims 1-4 obtained by the manufacturing method containing this.
[6] 錠剤の成分として使用される、請求項 1〜5のいずれか 1項に記載の杜仲葉水抽出 物分画物。  [6] The fraction of the Nakanaka leaf water extract according to any one of claims 1 to 5, which is used as a component of a tablet.
[7] 請求項 1〜6のいずれか 1項に記載の杜仲葉水抽出物分画物を含む医薬。  [7] A medicament comprising the fraction of the Nakanaka leaf water extract according to any one of claims 1 to 6.
[8] 水に溶解して飲料として使用される、請求項 1〜6のいずれか 1項に記載の杜仲葉 水抽出物分画物。 [8] The Tochu Naka water extract fraction according to any one of claims 1 to 6, which is used as a beverage after being dissolved in water.
[9] 請求項 1〜6および 8のいずれか 1項に記載の杜仲葉水抽出物分画物を含む食品  [9] A food comprising the fraction of the Nakanaka leaf water extract according to any one of claims 1 to 6 and 8.
[10] 機能性食品、健康食品、健康補助食品、栄養補助食品、保険機能食品、特定保 険用食品または栄養機能食品である、請求項 9に記載の食品。 [10] The food according to claim 9, which is a functional food, a health food, a health supplement, a nutritional supplement, an insurance functional food, a specified insurance food or a functional nutrition food.
[II] 請求項 1〜6のいずれか 1項に記載の杜仲葉水抽出物分画物の水溶液。  [II] An aqueous solution of the fraction of the Nakanaka leaf water extract according to any one of claims 1 to 6.
[12] 請求項 11に記載の水溶液を含む飲料。 [12] A beverage comprising the aqueous solution according to claim 11.
[13] 乾燥杜仲葉力 得られる杜仲葉水抽出物を逆相の合成吸着剤に吸着させ、水で 溶出する成分を得る工程を含む、杜仲葉水抽出物の分画物の製造方法。  [13] A method for producing a fraction of a dried nakanaka leaf extract, comprising the step of adsorbing the obtained nakanaka leaf water extract to a reverse-phase synthetic adsorbent to obtain a component eluted with water.
[14] 前記水で溶出する成分として得られる分画物を濃縮し、粉体を得る工程をさらに含 む、請求項 13に記載の製造方法。 [14] The method further includes a step of concentrating the fraction obtained as a component eluted with water to obtain a powder. The manufacturing method according to claim 13.
[15] 溶媒を留去した後の固形成分として、当該固形成分の総量に対しァスペル口サイド の含有量が 1重量％以下である、請求項 13または 14に記載の製造方法。 [15] The production method according to claim 13 or 14, wherein the content of the asper mouth side is 1% by weight or less based on the total amount of the solid component after the solvent is distilled off.
[16] ァスペル口サイドの含有量が固形成分の総量に対し 1重量％以下である、固体の 杜仲葉水抽出物分画物。 [16] A solid tsunaka leaf water extract fraction in which the content of the asperal mouth side is 1% by weight or less based on the total amount of solid components.
PCT/JP2007/054086 2006-03-03 2007-03-02 Fractionation product of aqueous extract of eucommia ulmoides oliver leaf, and composition for oral ingestion comprising the fractionation product WO2007102439A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2006058734A JP2007238456A (en) 2006-03-03 2006-03-03 Fractionated material of water extract of leaf of eucommia ulmoides oliver and antiobestic agent containing the same
JP2006-058734 2006-03-03

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JP2010273569A (en) * 2009-05-26 2010-12-09 Wasaburo Sato Drink and supplement each having metabolic syndrome-improving action
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JP5561984B2 (en) * 2009-09-29 2014-07-30 小林製薬株式会社 New beverage
JP5323003B2 (en) * 2010-06-01 2013-10-23 有限会社 碧山園 Manufacturing method for dried green leaves
JP5766173B2 (en) * 2012-12-14 2015-08-19 小林製薬株式会社 Foods and drinks containing chlorogenic acids
CN109045098A (en) * 2018-07-11 2018-12-21 贵州神奇药业有限公司 A kind of lower hyperlipidemia, hypertension, hyperglycemia lozenge and preparation method thereof

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JP2010273569A (en) * 2009-05-26 2010-12-09 Wasaburo Sato Drink and supplement each having metabolic syndrome-improving action
CN102670791A (en) * 2012-06-04 2012-09-19 黄芸 Eucommia bark Chinese medicinal oral solution for relieving high blood pressure and preparation method of eucommia bark Chinese medicinal oral solution

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