WO2007011622A3 - Microfluidic methods and apparatuses for sample preparation and analysis - Google Patents

Microfluidic methods and apparatuses for sample preparation and analysis Download PDF

Info

Publication number
WO2007011622A3
WO2007011622A3 PCT/US2006/027111 US2006027111W WO2007011622A3 WO 2007011622 A3 WO2007011622 A3 WO 2007011622A3 US 2006027111 W US2006027111 W US 2006027111W WO 2007011622 A3 WO2007011622 A3 WO 2007011622A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluid
targets
apparatuses
analysis
carrier fluid
Prior art date
Application number
PCT/US2006/027111
Other languages
French (fr)
Other versions
WO2007011622A2 (en
Inventor
Gregory R Yantz
Jonathan W Larson
Rudolf Gilmanshin
Original Assignee
Us Genomics Inc
Gregory R Yantz
Jonathan W Larson
Rudolf Gilmanshin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Genomics Inc, Gregory R Yantz, Jonathan W Larson, Rudolf Gilmanshin filed Critical Us Genomics Inc
Priority to EP06787065A priority Critical patent/EP1904653A4/en
Priority to JP2008522826A priority patent/JP2009501938A/en
Publication of WO2007011622A2 publication Critical patent/WO2007011622A2/en
Publication of WO2007011622A3 publication Critical patent/WO2007011622A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Nanotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Fluid Mechanics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Mathematical Physics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Theoretical Computer Science (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

Microfludic channels are constructed for use in preparing and/or analyzing samples. In one embodiment, a microfluidic channel receives a carrier fluid having both non-targets and targets. The non-targets are moved from the carrier fluid by diffusion and into sheathing fluids also present in the channel before contents of the carrier fluid are analyzed. In another embodiment, a microchannel delivers multiple fluid samples from an upstream portion to a downstream portion of the channel where each of the fluid samples are separated from one another by a sheathing fluid that also travels toward the downstream portion. In another embodiment, a microchannel is constructed to create multiple elongational flows in series that can align polymers from a coiled state.
PCT/US2006/027111 2005-07-18 2006-07-13 Microfluidic methods and apparatuses for sample preparation and analysis WO2007011622A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP06787065A EP1904653A4 (en) 2005-07-18 2006-07-13 Microfluidic methods and apparatuses for sample preparation and analysis
JP2008522826A JP2009501938A (en) 2005-07-18 2006-07-13 Microfluidic method and microfluidic device for sample preparation and analysis

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US70049005P 2005-07-18 2005-07-18
US70048005P 2005-07-18 2005-07-18
US70068905P 2005-07-18 2005-07-18
US60/700,480 2005-07-18
US60/700,689 2005-07-18
US60/700,490 2005-07-18

Publications (2)

Publication Number Publication Date
WO2007011622A2 WO2007011622A2 (en) 2007-01-25
WO2007011622A3 true WO2007011622A3 (en) 2007-05-24

Family

ID=37669351

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/027111 WO2007011622A2 (en) 2005-07-18 2006-07-13 Microfluidic methods and apparatuses for sample preparation and analysis

Country Status (3)

Country Link
EP (1) EP1904653A4 (en)
JP (1) JP2009501938A (en)
WO (1) WO2007011622A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9360476B2 (en) 2006-12-19 2016-06-07 Fio Corporation Microfluidic system and method to test for target molecules in a biological sample
US9459200B2 (en) 2008-08-29 2016-10-04 Fio Corporation Single-use handheld diagnostic test device, and an associated system and method for testing biological and environmental test samples

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3628729A3 (en) * 2007-07-16 2020-05-06 California Institute of Technology Arrays, devices, methods and systems for detecting target molecules
US8551763B2 (en) 2007-10-12 2013-10-08 Fio Corporation Flow focusing method and system for forming concentrated volumes of microbeads, and microbeads formed further thereto
US9792809B2 (en) 2008-06-25 2017-10-17 Fio Corporation Bio-threat alert system
EP2387721A4 (en) 2009-01-13 2014-05-14 Fio Corp A handheld diagnostic test device and method for use with an electronic device and a test cartridge in a rapid diagnostic test
US8202486B2 (en) * 2009-08-12 2012-06-19 Caliper Life Sciences, Inc. Pinching channels for fractionation of fragmented samples
WO2012040098A2 (en) 2010-09-21 2012-03-29 The University Of North Carolina At Chapel Hill Methods, systems and devices for forming nanochannels
US11053535B2 (en) 2011-09-12 2021-07-06 The University Of North Carolina At Chapel Hill Devices with a fluid transport nanochannel intersected by a fluid sensing nanochannel and related methods
EP3591408A1 (en) * 2012-02-10 2020-01-08 The University of North Carolina at Chapel Hill Devices with fluidic nanofunnels, associated methods, fabrication and analysis systems
JP6510984B2 (en) 2013-02-28 2019-05-08 ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒルＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｎｏｒｔｈ Ｃａｒｏｌｉｎａ Ａｔ Ｃｈａｐｅｌ Ｈｉｌｌ Nanofluidic device with integrated components for controlled capture, capture and transport of macromolecules, and related analysis methods
CN105452482B (en) 2013-03-13 2020-03-06 北卡罗来纳-查佩尔山大学 Nanofluidic devices for rapid mapping of whole genomes and related analysis systems and methods
EP3295148A4 (en) 2015-05-11 2018-11-07 The University of North Carolina at Chapel Hill Fluidic devices with nanoscale manifolds for molecular transport, related systems and methods of analysis
US11499183B2 (en) 2017-06-28 2022-11-15 Bio-Rad Laboratories, Inc. System and method for droplet detection
US11045805B2 (en) 2017-11-01 2021-06-29 Bio-Rad Laboratories, Inc. Microfluidic system and method for arranging objects
US10898895B2 (en) 2018-09-13 2021-01-26 Talis Biomedical Corporation Vented converging capillary biological sample port and reservoir
WO2020175381A1 (en) * 2019-02-27 2020-09-03 京セラ株式会社 Particle separation and measurement device, and particle separation and measurement apparatus

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4793705A (en) * 1987-10-07 1988-12-27 The United States Of America As Represented By The United States Department Of Energy Single molecule tracking
US5274240A (en) * 1990-01-12 1993-12-28 The Regents Of The University Of California Capillary array confocal fluorescence scanner and method
US5356776A (en) * 1991-09-10 1994-10-18 Hitachi, Ltd. DNA measuring method
US5674743A (en) * 1993-02-01 1997-10-07 Seq, Ltd. Methods and apparatus for DNA sequencing
US6355420B1 (en) * 1997-02-12 2002-03-12 Us Genomics Methods and products for analyzing polymers
US6790671B1 (en) * 1998-08-13 2004-09-14 Princeton University Optically characterizing polymers
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7118910B2 (en) * 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU770678B2 (en) * 1999-05-17 2004-02-26 Caliper Life Sciences, Inc. Focusing of microparticles in microfluidic systems
US6770182B1 (en) * 2000-11-14 2004-08-03 Sandia National Laboratories Method for producing a thin sample band in a microchannel device
JP2005514187A (en) * 2001-06-20 2005-05-19 サイトノーム インコーポレーテッド Microfluidic system including virtual wall fluidic interconnect ports for interconnecting fluids with microfluidic systems
US7318902B2 (en) * 2002-02-04 2008-01-15 Colorado School Of Mines Laminar flow-based separations of colloidal and cellular particles
EP1380337B1 (en) * 2002-07-12 2012-11-14 Tosoh Corporation Fine channel device and a chemically operating method for fluid using the device
EP1620203A2 (en) * 2003-04-10 2006-02-01 U.S. Genomics, Inc. Manipulation of polymers in a microchannel

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4793705A (en) * 1987-10-07 1988-12-27 The United States Of America As Represented By The United States Department Of Energy Single molecule tracking
US5274240A (en) * 1990-01-12 1993-12-28 The Regents Of The University Of California Capillary array confocal fluorescence scanner and method
US5356776A (en) * 1991-09-10 1994-10-18 Hitachi, Ltd. DNA measuring method
US5674743A (en) * 1993-02-01 1997-10-07 Seq, Ltd. Methods and apparatus for DNA sequencing
US6355420B1 (en) * 1997-02-12 2002-03-12 Us Genomics Methods and products for analyzing polymers
US6790671B1 (en) * 1998-08-13 2004-09-14 Princeton University Optically characterizing polymers
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6911345B2 (en) * 1999-06-28 2005-06-28 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7118910B2 (en) * 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1904653A4 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9360476B2 (en) 2006-12-19 2016-06-07 Fio Corporation Microfluidic system and method to test for target molecules in a biological sample
US9459200B2 (en) 2008-08-29 2016-10-04 Fio Corporation Single-use handheld diagnostic test device, and an associated system and method for testing biological and environmental test samples

Also Published As

Publication number Publication date
EP1904653A2 (en) 2008-04-02
WO2007011622A2 (en) 2007-01-25
JP2009501938A (en) 2009-01-22
EP1904653A4 (en) 2010-04-14

Similar Documents

Publication Publication Date Title
WO2007011622A3 (en) Microfluidic methods and apparatuses for sample preparation and analysis
AU2022200683A8 (en) Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US7314060B2 (en) Fluid flow conducting module
WO2002028507A3 (en) Electronic detection of interaction based on the interruption of flow
WO2009051821A3 (en) Sample preparation devices and analyzers
WO2007093902A8 (en) Assays
EP2227330B1 (en) Mobile rapid test system for nucleic acid analysis
WO2014152643A3 (en) Microfluidic microbe detection and isolation
GB201017905D0 (en) Preparation and analysis of samples
MY170587A (en) Centrifugal micro-fluidic device and method for immunoassay
EP2474360A3 (en) Microfluidic device and analyte detection method using the same
WO2009012340A3 (en) Microfluidic devices, methods and systems for detecting target molecules
WO2004061414A3 (en) Method and apparatus for splitting of specimens into multiple channels of a microfluidic device
WO2009014379A3 (en) Chip for analyzing fluids
WO2005029042A3 (en) Fabrication and packaging of suspended microchannel detectors
WO2008063758A3 (en) Multifunctional encoded particles for high-throughput analysis
AU2017390256A1 (en) Reagent channel mixing system and method
WO2019237742A1 (en) Microfluidic detection chip for multi-channel quick detecting
WO2008143646A3 (en) Device and method for digital multiplex pcr assays
WO2005003723A3 (en) Method for uniform application of fluid into a reactive reagent area
WO2006009724A3 (en) Microfluidic devices for fluid manipulation and analysis
CA2931582C (en) Microfluidic cartridge for molecular diagnosis, docking station using such a microfluidic cartridge, and process for analyzing a biological sample
WO2008052138A3 (en) Inline-injection microdevice and microfabricated integrated dna analysis system using same
WO2014058757A3 (en) Centripetal microfluidic platform for lal-reactive substances testing
WO2013126714A3 (en) Microfluidic cartridge

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2008522826

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2006787065

Country of ref document: EP