WO2006062273A1

WO2006062273A1 - Branched-amino acid supplement food

Info

Publication number: WO2006062273A1
Application number: PCT/KR2005/000517
Authority: WO
Inventors: Se-Gyu Kim; Ji-Hyun Kim
Original assignee: Se-Gyu Kim; Ji-Hyun Kim
Priority date: 2004-12-10
Filing date: 2005-02-25
Publication date: 2006-06-15
Also published as: KR20050034568A; KR100549697B1

Abstract

The present invention relates to a health supplement food utilizing, in particular, branched amino acids from among essential amino acids. More specifically, the present invention relates to: a health supplement food comprising branched chain amino acids (hereinafter, referred to as LIV) composed of leucine (L), isoleucine (I) and valine (V) which is made to have a composition similar to essential amino acids contained in naturally-occurring milk, eggs, soybeans, beef and the like, and enhancing instant impact power under anaerobic conditions, via addition of L- glutamine and taurine which are essentially required when a person is exposed to excessive exercise, stress or overwork; a health supplement food for improving a weak constitution, comprising herbal materials such as ginseng, red ginseng and Acanthopanax; and a diet food or beverage further comprising dietary fibers such as inulin, polydextrose and crystalline cellulose, and carnitine and hydroxy citric acid.

Description

BRANCHED-AMINO ACID SUPPLEMENT FOOD

Technical Field

[1] The present invention relates to a health supplement food utilizing, in particular, branched amino acids from among essential amino acids. More specifically, the present invention relates to: a health supplement food comprising branched chain amino acids (hereinafter, referred to as LIV) composed of leucine (L), isoleucine (I) and valine (V) which is made to have a composition similar to essential amino acids contained in naturally-occurring milk, eggs, soybeans, beef and the like, and enhancing instant impact power under anaerobic conditions, via addition of L-glutamine and taurine which are essentially required when a person is exposed to excessive exercise, stress or overwork; a health supplement food for improving a weak constitution, comprising herbal materials such as ginseng, red ginseng and Acanthopanax; and a diet food or beverage further comprising dietary fibers such as inulin, polydextrose and crystalline cellulose, and carnitine and hydroxy citric acid. Background Art

[2] Important factors that should be considered in exercises include, firstly mind control to do exercise, secondly proper nutrition and thirdly sufficient rest. Among the above-mentioned crucial factors, mind control to do exercise and taking sufficient rest can be solved by whether or not individual people make effort and make time for relaxation, but proper nutrition is another problem.

[3] Japanese Patent Laid-Open Publication No. Sho 60-049764 discloses a food composition for strengthening muscular power of an athlete, comprising amino acids composed of isoleucine:leucine:valine:arginine in a ratio of 1.3 to 0.6 : 1 : 0.4 to 0.8 : 1.4 to 0.6, and further proteins at a content of not less than 30%. Japanese Patent Laid- Open Publication No. Hei 9-052828 discloses an amino acid composition comprised essentially of leucine, wherein 5-20% by weight of valine, 5-20% by weight of leucine, 5-20% by weight of isoleucine, 3-20% by weight of lysine, 5-20% by weight of threonine, 3-20% by weight of methionine, 2-10% by weight of histidine, 1-10% by weight of phenylalanine, 1-10% by weight of tryptophan, 5-30% by weight of arginine, 5-30% by weight of glutamine and 2-10% by weight of proline are contained. WO 97/25883 discloses an amino acid preparation for stimulating hair growth, comprising the amino acids valine : leucine : isoleucine : proline in a ratio of 22:44:30:4. Korean Patent Laid-Open Publication No. 10-2003-0013836 (published on Feb. 15, 2003, Application No. 10-2001-0048037, assigned to the inventors of the present invention) discloses a nutritional supplement food for sportsmen, comprising a mixture of powdered maltodextrin, fructose, glucose, protein, collagen (gelatin), taurine, L- glutamine, citric acid, glucosamine, vitamins, porcine chondroitin, Vegetable Wasps and Plant Worms (Cordyceps), L-carnitine, amino silk, Karamix, guar gum, Genevia, octacosanol, gardenia green and fruit flavor.

[4] However, a variety of conventional food compositions or nutritional supplement foods as mentioned above contain various nutrients serving to supply a body energy by uptake thereof before and/or after taking part in strenuous exercise such as a marathon race requiring continuous running for a prolonged period of time or violent sports, but there has been a need for the development of a novel nutritional supplement food including branched chain amino acids in a proper composition ratio capable of preventing degradation of muscle glycogen of active people such as Taekwondo (Korean art of emptyhanded self-defense) practitioners, baseball players, judo players, wrestlers, basketball players and soccer players who require heavy muscular activities, or golf players, volleyballers and martial artists who require instant muscle power and thereby exhibiting no reduction of physical strength. Disclosure of Invention Technical Problem

[5] Calorie balance of athletes is determined by exercise and accompanied calorific consumption, and required amounts of nutrients vary depending upon intensity and time period of exercises and kinds thereof. In Korea, Recommended Dietary Allowances (RDA) for athletes is 1.5 to 2 times as that of ordinary people, and male athletes are recommended to take 3,000 to 5,000 kcal/day while female athletes are recommended to take 2,500 to 3,500 kcal/day. For required amounts of protein within the above range of RDA, endurance sport athletes, requiring protein amounts greater than 1.0 g/day/kg which is a daily requirement needed for ordinary people, are recommended to ingest 1.2 to 1.4 g/day/kg of BW, whereas high intensity sport athletes are recommended to ingest 1.4 to 1.8 g/day/kg of BW.

[6] Protein is one of particularly important nutrients for athletes and therefore athletes should ingest an adequate amount of protein. The reason why athletes should consume larger amounts of protein than ordinary people is to compensate for a break down of muscle into its original state and to induce active synthesis of protein at a recovery stage after performing exercise because protein is utilized as an energy source during exercise and particularly degradation of muscle protein is active and rapid for weight training.

[7] In compliance with a need for the development of a novel nutritional supplement food, the inventors of the present invention have paid attention to the fact that 35% of muscle cells consist of leucine, isoleucine and valine and branched chain amino acids (BCAAs) essential for the growth and development of muscles are essential amino acids which cannot be synthesized in the body, and therefore such branched chain amino acids should be supplied by foods or supplements.

[8] In particular, the present inventors have focused on the facts that the above- mentioned three branched chain amino acids assist in synthesis of other amino acids which are necessary for muscle composition and formation, promote production of insulin, and unlike other amino acids, undergoes fast absorption and metabolism in muscles rather than in the liver. Technical Solution

[9] Therefore, the present inventors have undertaken a great deal of research regarding composition of essential amino acids contained in a variety of foodstuffs and a minimum requirement of essential amino acids for infants, children and adults, respectively. Based on the results thus obtained, the present inventors have recognized the fact that absorption/digestion patterns of Koreans are dependent upon levels and composition of branched chain amino acids contained in dietary foods such as milk, eggs, soybean, beef, wheat and the like, rather than daily requirement of branched chain amino acids presented by Recommended Dietary Allowances (RDA) for Koreans and therefore have developed a supplement food in which branched chain amino acids are contained in compliance with an average ratio of branched chain amino acids contained in the above-mentioned foods.

[10] Further, the present inventors have developed a health supplement food for improving a weak constitution by adding herbal materials such as ginseng, red ginseng and Acanthopanax to branched chain amino acids, and have developed a health supplement food for dieting by addition of dietary fibers and appetite-related substances.

Advantageous Effects

[11] As described above, ingestion of a branched chain amino acid- supplemented food in accordance with the present invention by active people such as Taekwondo practitioners, golf players, martial artists, soccer players, baseball players, judo players, wrestlers, volleyballers and basketball players results in an increased level of essential amino acids in the serum of athletes and decreased decomposition of muscle glycogen, thereby providing inhibiting effects of decrease in physical strength.

[12] Further, since it is possible to achieve maximum enhancement of muscle power under anaerobic conditions, the supplement food in accordance with the present invention can be utilized as a health supplement food that improves athletic ability of leisure and professional sportsmen enjoying or taking part in individual competition events such as golf, Taekwondo and martial arts. In addition, the supplement food in accordance with the present invention can be utilized as a health supplement food that enhances adaptability of ordinary people to intensity of physical labor and results athletes being able to train more, thereby enhancing quality of labor and improving quality of exercise. Further, the supplement food in accordance with the present invention has effects regarding a decrease of blood glucose and increases amounts of amino acids in muscles and thereby can be utilized as a health supplement food such as a scientific nutritional agent for a diabetic. In addition, it is also expected that the supplement food in accordance with the present invention can be utilized as a health supplement food capable of overcoming a variety of disorders resulting from physical fatigue.

[13] Further, the health supplement food for improving a weak constitution comprising herbal materials such as ginseng, red ginseng and Acanthopanax is expected to have effects on people in need of improvement of weak constitution, maintenance of a healthy body and improvement of body functions.

[14] The diet food or beverage further comprising dietary fibers such as inulin, polydextrose and crystalline cellulose, and carnitine and hydroxy citric acid is expected to have significant effects on people who desire to follow a dieting regimen, particularly via loss of body fat around the abdomen region. Brief Description of the Drawings

[15] The above and other objects, features and other advantages of the present invention will be more clearly understood from the following detailed description taken in conjunction with the accompanying drawings, in which:

[16] Fig. 1 is a graph showing serum levels of amino acids in the respective experimental groups obtained from studies in accordance with the present invention; and

[17] Fig. 2 is a graph showing serum levels of amino acids in the respective experimental groups obtained from studies in accordance with the present invention. Best Mode for Carrying Out the Invention

[18] Hereinafter, a diet beverage containing LIV in accordance with the present invention will be described in more detail.

[19] The diet beverage is comprised of 5.5% inulin dietary fiber (dietary fiber content: more than 90%), 5% xylitol, 3.4% polydextrose (dietary fiber content: more than 90%), 1.5% Garcinia cambogia bark extract powder (HCA: more than 50%), 0.5% citric acid, 0.40% L-leucine, 0.26% L-valine, 0.25% L-glutamine, 0.22% L-isoleucine, 0.13% taurine, 0.1% pineapple flavor, 0.05% vitamin C, 0.03% L-carnitine, 0.02% purified salt, 0.004% aspartame, 0.004% sucralose, 0.001% grapefruit seed extract (DF- 100), 0.001% vitamin Bl hydrochloride, 0.001% vitamin B2, 0.001% vitamin B6 hydrochloride, and 82.628% purified water, thereby making the total of 100%. [20] The diet beverage containing dietary fibers as described above increases the quantity of amino acids in the muscle, inhibits absorption of fat, and activates intestinal motility by dietary fibers, which results in burning of abdominal fat and thus is helpful for dieting. Mode for the Invention

[21] In order to achieve the above-mentioned objects of the present invention, the inventors of the present invention have conducted a great deal of intensive study/ analysis and experiments on LIV such as leucine (L) which decreases blood glucose and is utilized as a nutrient in wound healing, isoleucine (I) which is necessary for formation of hemoglobin, and valine which converts glucose of the liver and muscle into energy.

[22] Although uptake of protein is absolutely necessary for improvement of physical strength, all the amounts of protein thus ingested are not always utilized in synthesis of muscle. When more protein is ingested than is necessary, the excess of protein cannot be stored in the body and consequently is burned as an energy source. Here, amino groups of amino acids are not completely combusted in the body and are then discharged from the body. When incompletely combusted amino groups are excreted, the amounts of discharged calcium are also increased, which affects metabolism of the liver and kidney. Therefore, as a measure to alleviate and/or offset negative effects on protein uptake of athletes, supplementation of LIV is necessary.

[23] LIV comprises about 70% of muscle proteins and is consumed during prolonged intensive exercise as like glycogen. As carbohydrates are known as an energy source that is primarily used when exercising, a great deal of attention has been directed to a LIV supplement known to offset against destruction of muscle proteins due to heavy exercise. However, there is not yet established an experimental protocol capable of eliciting exact results on studies of LIV, thus raising many different views. Many experts recognize the fact that an uptake of LIV after exercise has beneficial effects on recovery of physical strength.

[24] Characteristics and main functions of LIV are as follows:

[25] 1. Retardation of muscle degradation: LIV prevents muscle degradation

[26] 2. Improvement of nitrogen balance

[27] 3. Facilitation of energy production: LIV promotes production of energy in endurance-requiring sports

[28] 4. Increase in muscle synthesis

[29] 5. Energy source in muscle

[30] 6. Appropriate means to assist in muscle and energy metabolism

[31] 7. Assistance in protein synthesis and minimization of protein degradation [32] 8. Participation in recovery, growth and development of the muscular system [33] 9. Major caloric source of skeletal muscle tissues that are used during exercises [34] 10. Acceleration of protein synthesis and inhibition of protein degradation [35] 11. Three branched chain amino acids satisfy and offer necessary functions when they are concurrently ingested together

[36] 12. Increasing percent utilization of carbohydrates [37] 13. Assistance in formation of the muscular system by muscle cells [38] 14. Acceleration of protein synthesis in muscle cells [39] 15. Metabolized in muscle cells [40] 16. Leucine, isoleucine and valine should maintain balance between one another [41] [42] Leucine, isoleucine and valine are called branched chain amino acids as they are entangled like branched chains. In order to achieve key functions of these three essential amino acids, the most important is a balance and mixing ratio therebetween.

[43] In order to make the balance and harmony between three branched chain amino acids, the present inventors have analyzed LIV present in naturally-occurring products such as milk, eggs, soybeans, beef, wheat and the like others (see Table 1 below). In addition, reference has been made to minimal requirements of LIV among essential amino acids necessary for infants, children and adults (see Table 2 below).

[44] [45] [Table 1] [46] Content of LIV present in naturally-occurring food products [47]

[48] [Table 2] [49] Minimal requirement of LIV for humans (mg/kg of BD/day) [50]

[51] Since the minimal requirement of LIV for adults given in Table 2 is based on a final amount of LIV absorbed in the body, from the point of view that when the supplement food is orally administered and is finally absorbed into the body, the body can utilize desired nutrients, the present inventors have paid attention to the average ratio of LIV contained in dietary foods as set forth in Table 1. As a result, the present inventors have recognized the fact that absorption/digestion patterns of Koreans are governed by levels and composition of LIV contained in dietary foods such as milk, eggs, soybean, beef, wheat and the like which are commonly eaten and have developed a branched chain amino acid-supplemented food according to the average ratio of LIV of the above-mentioned foods.

[52] The health supplement food utilizing branched chain amino acids in accordance with the present invention comprises LIV composed of 45% by weight of leucine, 25% by weight of isoleucine and 30% by weight of valine.

[53] The above-mentioned LIV may be composed of 45 to 47% by weight of leucine

(L), 23 to 27% by weight of isoleucine (I) and 29 to 32% by weight of valine (V).

[54] It is also possible to prepare the health supplement food in accordance with the present invention by adding 70% LIV, and 20% L-glutamine, which promotes protein synthesis of muscles, thus assisting in fast growth of muscles, is capable of quickening the recovery from physical fatigue and prevents loss of muscles, 9% taurine that serves as an detoxifier having superior detoxifying effects on the liver and also exhibits superior efficacy on recovery from physical fatigue, and 1% a variety of vitamins and minerals.

[55] As previously discussed, leucine decreases blood glucose and is utilized as a nutrient in wound healing, isoleucine is necessary for formation of hemoglobin, and valine converts glucose of the liver and muscle into energy.

[56] The total branched chain amino acid- supplemented food comprises 65-75% LIV,

15 to 25% (20 to 35% of the weight of LIV) L-glutamine and 5 to 15% (7 to 20% of the weight of LIV) taurine that are necessary when a person is exposed to excessive exercise, stress or overwork, and less than 2% (0.01 to 2.9% of the weight of LIV) various other vitamins that are anti-oxidant nutrients and are necessary to assist in absorption and metabolism of LIV, and minerals.

[57] Further, it is possible to prepare a diet beverage by addition of dietary fibers such as inulin, polydextrose and crystalline cellulose to the LIV, or by addition of L- carnitine and hydroxy citric acid to the LIV. In addition, it is possible to prepare a health supplement food for improving a weak constitution, by addition of herbal materials such as ginseng, red ginseng and Acanthopanax to the LIV

[58] L-glutamine promotes protein synthesis of muscles, prevents loss of muscles, accelerates a recovery rate of muscles, and assists in fast growth of muscles. L-glutamine is a free amino acid abundantly found in the body and comprises 60% of the total amino acids therein. In addition, L-glutamine is synthesized in the body by other amino acids, but is a conditionally-essential amino acid that must be ingested to maintain muscles and prevent a decline of physical strength in athletes since glutamine in muscles is quickly utilized when the body is exposed to stress due to physical training and exercise.

[59] Taurine is aminoethylsulfonic acid having properties of ampholyte like amino acids, and exhibits production of bile acid, regulation of cholesterol concentration, regulation of cell membrane ion permeability, anti-oxidative action and inhibition of excessive nerve excitation, as main physiological functions. Taurine is mainly found in cells of cardiac and skeletal muscles, brain and genital organs rather than in body fluid. Taurine has been known as a cardiotonic agent because it weakens contractive force of the heart when a concentration of calcium ions in the heart is below a normal level. In addition, taurine reacts with bile acid to form a polymer, thereby alleviating toxicity of bile acid, and assists in absorption of fats in the intestines. Taurine protects cells by removing peroxides and nitrogen oxides, and free groups before they damage the cell membranes. Taurine is most abundant among amino acids present in the brain, and also exhibits nervous sedative effects via inhibition of excessive nerve excitation when nerves are continuously excited, thereby diminishing stress due to exercise.

[60] Therefore, the present invention has improved functions of the branched chain amino acid- supplemented food by adding L-glutamine and taurine, which are essentially required when exposed to excessive exercise, stress or overwork, to LIV.

[61] The branched chain amino acid- supplemented food has contained L-glutamine in an amount of 20 to 35% of the weight of LIV and taurine in an amount of 7 to 20% of the weight of LIV.

[62] Dietary fibers such as inulin, polydextrose and crystalline cellulose affect bacteria in the large intestine and therefore inhibit action of carcinogenic substances, thus preventing incidence of colon cancer. Those dietary fibers block absorption of cholesterol, thus preventing occurrence of adult diseases, alleviates the feeling of emptiness and retards absorption of foods, thereby filtering cholesterol. Dietary fibers combine with salts which are then discharged outside the body, thereby hindering elevation of blood pressure which is helpful for treatment and prevention of diabetes. Even for obese people, sufficient uptake of fibrous materials provides them a feeling of fullness while inhibiting absorption and digestion of nutrients, thus helping them refrain from excessive eating.

[63] L-carnitine is a coenzyme that plays an important role in fatty acid metabolism and is an amino acid derivative. Hydroxy citric acid suppresses appetite, assists in degradation of body fats and blocks synthesis of fats.

[64] The present invention has prepared a health supplement food for dieting by adding dietary fibers such as inulin, polydextrose and crystalline cellulose, and L-carnitine and hydroxy citric acid to LIV.

[65] Ginseng, red ginseng and Acanthopanax heighten vitality of the body and mind, thereby improving a weak constitution, are helpful for recovery from fatigue, activate production of blood in the body, thereby making blood circulation constant and favorable, improve functions or development of the body, calm and stabilize the mind and sprit, thereby strengthening functions of the heart, are useful in getting rid of neurasthenia, neurosis and stress, replenish the body fluid to appease thirst, are useful for strengthening functions of the spleen and diabetes, protect functions of the lung, thereby relieving coughing, assist in prevention and treatment of respiratory diseases, strengthen the stomach and intestines, thus promoting appetite, bind the bowel (stopping diarrhea), reinforce functions of the stomach and intestines, eliminate toxins in the body, thereby rendering rough skin fine and smooth, consequently being efficacious for skin diseases, and relieve furuncles, thereby resulting in detoxification and enhanced resistance against diseases.

[66] Therefore, the present invention provides a health supplement food for improving a weak constitution, prepared by adding ginseng, red ginseng and Acanthopanax to LIV.

[67] Athletes need to ingest anti-oxidant nutrients in a proper amount and examples of such anti-oxidant nutrients include vitamin C, E and -carotene. Representative vitamin C is important for synthesis of collagen (myofiber protein) of connective tissues and assists in absorption and digestion of minerals and iron constituting a cytochrome system which is involved in energy metabolism. Vitamin C removes histamine, a substance which is secreted in response to stress, and therefore plays a crucial role to control conditions of athletes who are tired from consecutive matches.

[68] Active oxygen species, which are by-products produced during biological activity of the body, normally occur in energy production in the body, metabolic processes of lipids or proteins, catecholamine responses and a variety of inflammatory reactions, but most of them are neutralized by anti-oxidants. In addition, radicals and peroxides are known to play a very important role in pathogenesis of various diseases, i.e., cardiovascular diseases such as hypertension, arteriosclerosis and coronary artery diseases, malignant tumors, chronic inflammatory diseases, Parkinson's syndrome, epilepsy, various cerebral trauma, cataracts, skin cancers and auto-immune diseases.

[69] During exercise, oxygen demand is sharply increased in the body. Since muscles in action consume large quantities of oxygen while producing significant amounts of active oxygen species which in turn damage cell membrane structures, many researchers have elucidated that such damage is correlated with increased products of oxidation processes immediately after exercising.

[70] The active oxygen species, generated by continuous prolonged exercises under the conditions at which athletes are subjected to heavy stress, lead to changes in fluidity, permeability and excitability of cell membranes or changes in functions of enzymes bound to the cell membranes. Generally, it can be said that all of the materials constituting the body are susceptible to oxidative stress by active oxygen species, and representative examples of such materials include lipids, proteins, DNA, nucleic acids and enzymes. When those materials have suffered oxidative damage, this results in deterioration of cell functions. In addition, since high intensity exercise require as much as 20 times more oxygen than an average oxygen demand of the muscle, sharply-elevated oxygen demand leads to hypoxia and accumulation of lactic acid, thereby causing fatigue of muscles.

[71] Even though high intensity exercise lead to increased active oxygen species which then leads to destruction of cells, thus resulting in acceleration of aging, such active oxygen species are neutralized by antioxidant nutrients and it is possible to achieve much effects by kinds and intensity of exercises and control of duration thereof.

[72] Therefore, there were added nine species of vitamins and 5 species of minerals which are necessary for exerting the above-mentioned anti-oxidative action and assisting in absorption and metabolism of LIV. The above vitamins and minerals include vitamin A, vitamin C, vitamin B 1 hydrochloride, vitamin B2, vitamin B6 hydrochloride, vitamin B 12, vitamin D3, folic acid, nicotinic amide, calcium phosphate, iron, magnesium oxide, zinc oxide and maltodextrin.

[73] In order to confirm efficacy of the branched chain amino acid-supplemented food,

48 male athletes, who are playing an active part in Taekwondo in K and Y universities of Korea, were divided into three groups depending on uptake of LIV, i.e. a placebo group (LIV 0 g/day administered, designated PSG ), a half-supplemented group (LIV 2.5g/day administered, designated HSG ), and a full- supplemented group (LIV 5.0g/day administered, designated FSG ), each consisting of 16 individuals, and were orally administered the branched chain amino acid-supplemented food for 12 weeks. [74] The thus-supplied LIV was a supplement food containing 70% LIV/capsule and having a composition ratio of isoleucine, leucine and valine in a range of 1:1.8:1.2.

[75] Since the Recommended Dietary Allowances (RDA) of LIV for athletes have not been suggested yet, two-times a daily RDA for ordinary people was taken as the daily requirement of LIV and was ingested by athletes immediately before and after exercise.

[76] Study period has ranged from January 7, 2002 (winter camp training began) to

March 30, 2002. On average, training was performed including an early-morning exercise (6:00 to 7:00, walk and double-quick), a forenoon exercise (10:00 to 12:00), an afternoon exercise (15:00 to 17:30) and a night exercise (19:30 to 21:00), for a week, with only a forenoon exercise on Saturday, and individual exercise on Sunday.

[77]

[78] Serum level of amino acids

[79]

[80] Graphs on serum levels of amino acids are shown in Figs. 1 and 2.

[81] Fig. 1 is for essential amino acids, whereas Fig. 2 is for non-essential amino acids.

[82] Analysis results of blood showed that isoleucine was significantly increased in all the groups after supplementation of the LIV, whereas leucine and valine were increased in HSG and FSG only.

[83] The essential amino acid, histidine was significantly decreased in PSG and HSG after supplementation of the LIV, but was significantly increased in FSG. Lysine, methionine and phenylalanine were significantly increased in all the groups after supplementation of the LIV, whereas threonine was significantly increased in HSG and FSG.

[84] For non-essential amino acids, alanine, glycine and tyrosine were significantly increased in HSG and FSG, whereas arginine and serine were significantly increased in FSG only. Aspartic acid and glutamine were significantly increased in all the groups after supplementation of the LIV.

[85]

[86] Maximum anaerobic power

[87]

[88] Examination results on the maximum anaerobic power are shown in Table 3.

[89] Before supplementation of the LIV, PSG exhibited 1,318.1+159.9 watt, HSG and

FSG exhibited 1,402.1+177.9 watt and 1,346.9+150.0 watt, respectively, and thus there was no difference between three groups. However, in week 12 after supplementation, PSG exhibited 1,300.3+130.3 watt, and HSG and FSG exhibited 1,401.1+239.2 watt and 1,445.4+161.3 watt, respectively, and the maximum anaerobic power was significantly higher in FSG (P < 0.05). In addition, the maximum anaerobic power was significantly increased in FSG after supplementation of the LIV than before supplementation of the LIV (P<0.01).

[90] When the maximum anaerobic power was divided by body weight, prior to supplementation of the LIV, PSG exhibited 17.9+2.1 watt/kg, and HSG and FSG exhibited 18.8+2.3 watt/kg and 18.7+1.6 watt/kg, respectively, and thus there was no difference between three groups. However, in week 12 after supplementation, PSG exhibited 17.5+12.7 watt/kg, and HSG and FSG exhibited 18.7+1.8 watt/kg and 20.4+2.3 watt/ kg, respectively, and the maximum anaerobic power was significantly higher in FSG (P < 0.05). In addition, the maximum anaerobic power was significantly increased in FSG after supplementation of the LIV than before supplementation of the LIV (P< 0.01).

[91] [92] [Table 3] [93] Comparison of maximum anaerobic power between respective groups [94]

Group

Variable Time PSG^1} HSG²⁾ FSG³⁾

Power pre 1318.1±159.9 1402.1±177.9 1346.9±150.0

(watt) post 1300.3±130.3^b 1401.1±239.2^ab 1445.4±161.3^a**

Power pre 17.9±2.1 18.7±1.6 18.8±2.3

(watt/kg) post 17.5±2.7^b 18.7±1.8^ϊb 20.4±2.3"^**

All values are MeantSD

Letters with different superscripts in the same raw are significantly different (p<0.05) among the groups by Duncan's multiple range test. ** P<0.01 statistically difference between Owk and 12wk maximum anaerobic power

1) PSG : Placebo Supplement Group

2) HSG : BCAA 2.5g/day Supplement Group

3) FSG : BCAA 50g/day Supplement Group

[95] [96] Therefore, it was determined that the group (FSG) to which 5.0g/day of LIV was administered exhibits significant increases in leucine, isoleucine and valine, as well as other amino acids, thereby improving maximum anaerobic power. Industrial Applicability

[97] As apparent from the above description, a health supplement food utilizing branched chain amino acids in accordance with the present invention is needed by athletes and ordinary people. The health supplement food can be utilized to produce a health supplement food for improving a weak constitution, by inclusion of herbal materials such as ginseng, red ginseng and Acanthopanax, and to produce a diet food or beverage and particularly a food or beverage for removing body fat around the abdomen region, by further inclusion of dietary fibers such as inulin, polydextrose and crystalline cellulose, and carnitine and hydroxy citric acid.

[98] Although the preferred embodiments of the present invention have been disclosed for illustrative purposes, those skilled in the art will appreciate that various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims.

[99]

Claims

[I] A branched chain amino acid-supplemented food using branched chain amino acids, comprising LIV composed of:

45 to 47% by weight of leucine (L),

23 to 27% by weight of isoleucine (I), and

29 to 32% by weight of valine (V). [2] The food according to claim 1, wherein L-glutamine and taurine are further included in the LIV. [3] The food according to claim 2, wherein L-glutamine is 20 to 35% of the weight of the LIV, and taurine is 7 to 20% of the weight of the LIV. [4] The food according to any one of claims 1 to 3, wherein herbal materials such as ginseng, red ginseng and Acanthopanax are further included in the LIV. [5] The food according to claim 4, wherein dietary fibers such as inulin, polydextrose and crystalline cellulose are further included. [6] The food according to any one of claims 1 to 3, wherein dietary fibers such as inulin, polydextrose and crystalline cellulose are further included in the LIV. [7] The food according to claim 6, wherein carnitine and hydroxy citric acid are further included. [8] The food according to claim 7, wherein herbal materials such as ginseng, red ginseng and Acanthopanax are further included. [9] The food according to any one of claims 1 to 3, wherein a vitamin and mineral are further included in an amount of 0.01 to 2.9% of the weight of the LIV. [10] The food according to claim 9, wherein the vitamin and mineral are selected from vitamin A, vitamin C, vitamin Bl hydrochloride, vitamin B2, vitamin B6 hydrochloride, vitamin B 12, vitamin D3, folic acid, nicotinic amide, calcium phosphate, iron, magnesium oxide, zinc oxide and maltodextrin.

[I I] The food according to any one of claims 1 to 10, wherein the food has characteristics of powder, granular powder, tablet, soft capsule, hard capsule, beverage, gel and any combination thereof.