WO2005040398A3 - Methods for preserving nucleated mammalian cells - Google Patents

Methods for preserving nucleated mammalian cells Download PDF

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Publication number
WO2005040398A3
WO2005040398A3 PCT/US2004/034605 US2004034605W WO2005040398A3 WO 2005040398 A3 WO2005040398 A3 WO 2005040398A3 US 2004034605 W US2004034605 W US 2004034605W WO 2005040398 A3 WO2005040398 A3 WO 2005040398A3
Authority
WO
WIPO (PCT)
Prior art keywords
mammalian cells
methods
cells
nucleated mammalian
preserving
Prior art date
Application number
PCT/US2004/034605
Other languages
French (fr)
Other versions
WO2005040398A2 (en
WO2005040398A9 (en
Inventor
John H Crowe
Fern Tablin
Ann E Oliver
Kamran Jamil
Xiaocui Ma
James S Clegg
Willem Wolkers
Thurein Htoo
Original Assignee
Univ California
John H Crowe
Fern Tablin
Ann E Oliver
Kamran Jamil
Xiaocui Ma
James S Clegg
Willem Wolkers
Thurein Htoo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/721,678 external-priority patent/US20040185524A1/en
Priority claimed from US10/722,154 external-priority patent/US20050112686A1/en
Application filed by Univ California, John H Crowe, Fern Tablin, Ann E Oliver, Kamran Jamil, Xiaocui Ma, James S Clegg, Willem Wolkers, Thurein Htoo filed Critical Univ California
Priority to US10/575,832 priority Critical patent/US20070026377A1/en
Publication of WO2005040398A2 publication Critical patent/WO2005040398A2/en
Publication of WO2005040398A9 publication Critical patent/WO2005040398A9/en
Publication of WO2005040398A3 publication Critical patent/WO2005040398A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients

Abstract

Methods and compositions are provided for increasing the survival of nucleated mammalian cells following drying and rehydration. The methods include introducing a disaccharide such as trehalose into said cells, optionally including heat shock proteins, apoptosis inhibitors, and arbutin, drying said cells, and rehydrating them. The invention further provides nucleated mammalian cells that have increased capacity to survive, divide and, in some cases, differentiate, following drying and rehydration. The cells comprise a disaccharide and one or more of the following: a heat shock protein, an apoptosis inhibitor, and arbutin.
PCT/US2004/034605 2000-02-10 2004-10-18 Methods for preserving nucleated mammalian cells WO2005040398A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US10/575,832 US20070026377A1 (en) 2000-02-10 2004-10-18 Methods for preserving nucleated mammalian cells

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US68690403A 2003-10-16 2003-10-16
US10/686,904 2003-10-16
US72155703A 2003-11-25 2003-11-25
US10/721,557 2003-11-25
US10/721,678 2003-11-25
US10/721,678 US20040185524A1 (en) 2000-02-10 2003-11-25 Biological samples and method for increasing survival of biological samples
US10/722,154 US20050112686A1 (en) 2003-11-25 2003-11-25 Method for introducing molecules into biological samples
US10/722,154 2003-11-25

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US10/721,678 Continuation US20040185524A1 (en) 2000-02-10 2003-11-25 Biological samples and method for increasing survival of biological samples

Publications (3)

Publication Number Publication Date
WO2005040398A2 WO2005040398A2 (en) 2005-05-06
WO2005040398A9 WO2005040398A9 (en) 2005-08-25
WO2005040398A3 true WO2005040398A3 (en) 2006-01-26

Family

ID=34528256

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/034605 WO2005040398A2 (en) 2000-02-10 2004-10-18 Methods for preserving nucleated mammalian cells

Country Status (1)

Country Link
WO (1) WO2005040398A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1956898A2 (en) 2005-11-30 2008-08-20 Massachusetts Institute of Technology Pathogen-detecting cell preservation systems
JP5394932B2 (en) * 2007-11-02 2014-01-22 日本ケミカルリサーチ株式会社 Pharmaceutical composition containing human mesenchymal stem cells
CN102487938B (en) * 2011-11-15 2014-04-02 青岛大学医学院附属医院 Cryopreservation method for protecting cell junction
BR102014002362A8 (en) * 2014-01-30 2018-04-17 Uniao Brasileira De Educacao E Assistencia Mantenedora Da Puc Rs method of immunomodulation and / or preservation of ex vivo organs, compositions, process and uses
US11297828B2 (en) 2016-03-14 2022-04-12 The Regents Of The University Of Michigan Surface tension mediated lyo-processing technique for preservation of biologics
CN113481207B (en) * 2021-07-15 2023-04-07 西南大学 Essential gene Bmtret1 for silkworm egg diapause and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5827741A (en) * 1996-11-19 1998-10-27 The Regents Of The University Of California Cryopreservation of human adult and fetal pancreatic cells and human platelets
US6127177A (en) * 1998-09-11 2000-10-03 Massachusetts Institute Of Technology Controlled reversible poration for preservation of biological materials
US20020076445A1 (en) * 2000-02-10 2002-06-20 Crowe John H. Eukaryotic cells and method for preserving cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5827741A (en) * 1996-11-19 1998-10-27 The Regents Of The University Of California Cryopreservation of human adult and fetal pancreatic cells and human platelets
US6127177A (en) * 1998-09-11 2000-10-03 Massachusetts Institute Of Technology Controlled reversible poration for preservation of biological materials
US20020076445A1 (en) * 2000-02-10 2002-06-20 Crowe John H. Eukaryotic cells and method for preserving cells

Also Published As

Publication number Publication date
WO2005040398A2 (en) 2005-05-06
WO2005040398A9 (en) 2005-08-25

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