WO2004064852B1 - Standardised steroid saponin mixture, a method of its obtaining and application - Google Patents
Standardised steroid saponin mixture, a method of its obtaining and applicationInfo
- Publication number
- WO2004064852B1 WO2004064852B1 PCT/BG2003/000043 BG0300043W WO2004064852B1 WO 2004064852 B1 WO2004064852 B1 WO 2004064852B1 BG 0300043 W BG0300043 W BG 0300043W WO 2004064852 B1 WO2004064852 B1 WO 2004064852B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- standardised
- water
- extraction
- butanol
- steroid saponin
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims abstract 5
- 150000005856 steroid saponins Chemical class 0.000 title claims abstract 4
- 238000000034 method Methods 0.000 title claims abstract 3
- 229930182490 saponin Natural products 0.000 claims abstract 2
- 150000007949 saponins Chemical class 0.000 claims abstract 2
- 235000017709 saponins Nutrition 0.000 claims abstract 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims 9
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical class CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims 4
- 238000000605 extraction Methods 0.000 claims 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 4
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims 3
- 238000001035 drying Methods 0.000 claims 3
- 238000005406 washing Methods 0.000 claims 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims 2
- 241001521901 Tribulus lanuginosus Species 0.000 claims 2
- 238000001914 filtration Methods 0.000 claims 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 239000002021 butanolic extract Substances 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 239000012141 concentrate Substances 0.000 claims 1
- 239000003814 drug Substances 0.000 claims 1
- 229940079593 drug Drugs 0.000 claims 1
- 239000000284 extract Substances 0.000 claims 1
- 230000002519 immonomodulatory effect Effects 0.000 claims 1
- 230000003308 immunostimulating effect Effects 0.000 claims 1
- 239000003960 organic solvent Substances 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 239000008213 purified water Substances 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
- 241001533104 Tribulus terrestris Species 0.000 abstract 1
- 239000002955 immunomodulating agent Substances 0.000 abstract 1
- 229940121354 immunomodulator Drugs 0.000 abstract 1
- 230000002584 immunomodulator Effects 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Immunology (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Steroid Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a standardised steroid saponin mixture obtained from the epigeous part of Tribulus terrestris L., containing more than 80 per cent of furostanol saponins, a method of obtainment and its application as an immunostimulator and immunomodulator.
Claims
1. Standardised steroid saponin mixture, obtained from the epigeous part of Tribulus terrestris L, containing more than 80 per cent of furostanol saponins.
2. A method to obtain a standardised steroid saponin mixture as the one described in Claim 1 , from Tribulus terrestris by the means of extraction with water-saturated n-butanol, concentration and drying, characterised in that the first extraction being performed using aqueous methanol in a 1 :1 water / methanol ratio at a temperature of 52 to 55°C, followed by purification with active carbon and filtering; after concentration and full removal of the methanol and subsequent extraction with methylene chloride, the purified water extract is acidified up to pH 3 - 5 using hydrochloric acid, then, after extraction with n-butanol and concentration it is proceeded either to: dropping into acetone, leaving for about 24 hours at a temperature of 10 to 15°C, filtering, acetone washing, and drying or to: washing the concentrate with 0.5 - 1.5-per cent sodium hydroxide solution and, after separating the two layers, washing the butanol layer with water up to pH 7, vacuum-concentrating the butanol extracts until the organic solvent is fully removed, and drying the water solution.
3. Application of the standardised mixture described in Claim 1 in the synthesis of drugs with immunomodulating and/or immunostimulating effect.
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAA200508283A UA80182C2 (en) | 2003-01-24 | 2003-11-12 | Mixture of steroid saponins obtained from tribulus terrestris l. with immunomodulating properties, a method of its obtaining and application |
| EEP200500024A EE05443B1 (en) | 2003-01-24 | 2003-12-11 | Mixture of standardized steroidal saponins, method of preparation and use |
| GB0513893A GB2412867B (en) | 2003-01-24 | 2003-12-11 | Standardised steroid saponin mixture, a method of its obtaining and application |
| EA200501170A EA008763B1 (en) | 2003-01-24 | 2003-12-11 | Standardised steroid saponin mixture, a method of its obtaining and application |
| AU2003286011A AU2003286011A1 (en) | 2003-01-24 | 2003-12-11 | Standardised steroid saponin mixture, a method of its obtaining and application |
| HR20050614A HRPK20050614B3 (en) | 2003-01-24 | 2005-07-01 | Standardised steroid saponin mixture, a method of its obtaining and application |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BG107499 | 2003-01-24 | ||
| BG107499A BG65737B1 (en) | 2003-01-24 | 2003-01-24 | Standardized mixture of steroid saponins, method for the preparation and apllication thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2004064852A1 WO2004064852A1 (en) | 2004-08-05 |
| WO2004064852B1 true WO2004064852B1 (en) | 2004-10-07 |
Family
ID=32739195
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/BG2003/000043 WO2004064852A1 (en) | 2003-01-24 | 2003-12-11 | Standardised steroid saponin mixture, a method of its obtaining and application |
Country Status (10)
| Country | Link |
|---|---|
| AU (1) | AU2003286011A1 (en) |
| BG (1) | BG65737B1 (en) |
| EA (1) | EA008763B1 (en) |
| EE (1) | EE05443B1 (en) |
| GB (1) | GB2412867B (en) |
| HR (1) | HRPK20050614B3 (en) |
| LV (1) | LV13366B (en) |
| PL (1) | PL377142A1 (en) |
| UA (1) | UA80182C2 (en) |
| WO (1) | WO2004064852A1 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080182801A1 (en) | 2003-12-22 | 2008-07-31 | Btg International Limited | Core 2 glcnac-t inhibitors |
| GB0329667D0 (en) | 2003-12-22 | 2004-01-28 | King S College London | Core 2 GlcNAc-T inhibitor |
| GB0513881D0 (en) | 2005-07-06 | 2005-08-10 | Btg Int Ltd | Core 2 GLCNAC-T Inhibitors III |
| GB0513888D0 (en) | 2005-07-06 | 2005-08-10 | Btg Int Ltd | Core 2 GLCNAC-T Inhibitors II |
| CN103040880B (en) * | 2012-12-13 | 2017-10-10 | 大兴安岭林格贝寒带生物科技股份有限公司 | A kind of isolation and purification method of tribuloside |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BG52085B2 (en) * | 1985-01-18 | 1996-06-28 | Tomova | Method for the isolation of standardized mixture of steroid saponins |
| US6343258B1 (en) * | 1999-08-13 | 2002-01-29 | ALEXIS Brian | Method for testing for readiness for harvesting of tribulus terrestris l. having high steroidal saponin content |
-
2003
- 2003-01-24 BG BG107499A patent/BG65737B1/en unknown
- 2003-11-12 UA UAA200508283A patent/UA80182C2/en unknown
- 2003-12-11 GB GB0513893A patent/GB2412867B/en not_active Expired - Fee Related
- 2003-12-11 PL PL377142A patent/PL377142A1/en unknown
- 2003-12-11 EA EA200501170A patent/EA008763B1/en unknown
- 2003-12-11 AU AU2003286011A patent/AU2003286011A1/en not_active Abandoned
- 2003-12-11 EE EEP200500024A patent/EE05443B1/en unknown
- 2003-12-11 WO PCT/BG2003/000043 patent/WO2004064852A1/en not_active Application Discontinuation
-
2005
- 2005-07-01 HR HR20050614A patent/HRPK20050614B3/en not_active IP Right Cessation
- 2005-08-16 LV LVP-05-96A patent/LV13366B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| LV13366B (en) | 2006-03-20 |
| EE200500024A (en) | 2005-10-17 |
| EE05443B1 (en) | 2011-08-15 |
| UA80182C2 (en) | 2007-08-27 |
| WO2004064852A1 (en) | 2004-08-05 |
| BG65737B1 (en) | 2009-09-30 |
| EA200501170A1 (en) | 2005-12-29 |
| EA008763B1 (en) | 2007-08-31 |
| AU2003286011A1 (en) | 2004-08-13 |
| GB2412867B (en) | 2007-07-04 |
| BG107499A (en) | 2004-08-31 |
| GB0513893D0 (en) | 2005-08-10 |
| PL377142A1 (en) | 2006-01-23 |
| HRPK20050614B3 (en) | 2007-10-31 |
| GB2412867A (en) | 2005-10-12 |
| HRP20050614A2 (en) | 2006-06-30 |
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